Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0016695

Physical Location & Seq

Genomic Coordinates Forward (+)

15039985 .. 15043508

Gene Structure 3524 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g17460.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2835 bp

ATGAGAATCGCCGAGATGTCAACGCCGGAGCTCCGGCAAAGCCACAACGACTCCGCCTCCCAACCGCAACCTCATCAGCACCTGATCTCCCAGATCGAGTCCTCAGTCAACCAAACCGAGAGCCTCTCCCCTGAGAATCTCATACCGGACACAATCTCCGCCGATCTCCGGCGAGCCTTGACTCAACTGAGCCACCACGCTCCGTTCCCCAACTCTCTCAAGCTCGTCATCTGGAAACTCAGTTACCGCCTCTGGAACGCCTGCGTCGATCTCTCCAACACCACCTCCCTCCGCTCCCTCCCTTCCTCCAAAGCCGAAGAGCACGCCAAGCTCCGCCACATCGCCGCCGACCTACTCTTCGTCGCCGGTGACGTTTCCGGCGTTCCTTCCCCGGCGATCAAGTCCGCCTCGTTCTACCACAAGACCGGCGTCAAATGGCACGAGCTTCGGAAATTCGACCTCGCTTCGTCGTGCTTCGAGAAAGCCACCGATCTGCTCTCGAAGATCGGAATCGACACAGTCTCCGACGCCGGCGAGAAGAAGCTGTTTCTGGATTTGAGCATCGCTAGGTCAAAAACCGCCTGGGAGGTATCGGACCGGAATCTCGCCGTGGCGCTGCTGAACCGGGCCAAGAGCTTGCTGTTCGGGTCGCCGGAGCACTACAAAACCCTAGCCAGTCAGTACTCGTCGTTCGGAAAGAGCGCTCTGTCGAACAGCGAGAGTAGCTCTCTCAACGAAGCGTTGAAGCTCATGAACGAGGCTCTGGAGCTCTACGAGAAGGGGCTGCGCGTGGCGAGGACGCGCGAAGAGACGGCGGATCTCAAGGCGTTAAGGTCGAAGACGCTGCGGTTCATCTCGGCCGTTCATTTGCAGATGAACGAGTTCGAGAGCGTGATCAAGTGCGTGAGGGTGTTGCGAGACGGCGACGCAGGTGATCAGCACCCTAGCCTTCCGGTGATGGCGATGAAGGCGTGGTTGGGGTTAGGGAAGTACGGCGAGGCCGAGAAGGAGCTCAGGGGCATGGTGGTAAATAACGGCATTCCCGAGGGGGCTTGGGTTTCGGCGGTGGAGGCTTACTTTGAGTCGGCCGGGACGGCTGGAGCGGAGACGGCGAAGGGAGTGTTTTTAGGGCTGCTTGGGAGGTGCCACGTCAGCGCCAGCGCGGCGGTTAGGGTTTCTCACAGGGTTCTTGGGGAGAGTTGCAGTGAGGGATCAAAAGTGAGGGCTAAGGTGGTTTCGGAGCTTGTATCTGATGACAGAGTGGTGGCTCTCTTCTCCGGTGAGGCTGCTGCGAAGCAGAGGACTGCAATGCATGCTGTGCTTTGGAATTGTGCTGCTGATCATTTCCGGTTGAAAGACTATGTAACAAGTGCAGACCTGTTTGAGAAAGCAATGCTGTATATCCCATTCGACATAGAGAATCGAATTCTTCGAGCCAAGGGCTTCAGAGTATTGTGTCTCTGCCACTTGGGTCTTTCCCACCTTGATCAAGCTCAAGAATACATCAATGAGGCTGAAAAGCTTGAACCTAACATTGCTTCAGCTTTCCTAAAGTACAAGATTTATCTGCAGAAGAAAGACCAGGATGGGGCTATTAATCAGATTCAGGCAATGACAACCTGCCTTGATTTTACGCCAGACTTTCTCTCTCTTGCGGCCCATGAAGCTGTTGCCTGCCGTGCTCTTGCTATTGCTGTTGCTGCACTATCGAATTTACTGAACTTTTATGCCCCAGGGAAATCCATGCCAACAAGTGAAGTTGTAGTTCTGCGCACCTTGGTCACAATCCTTACTCAAGAACCTGGGAATGAGCTTGAAGCCCTTAAGTTTGTTAAGCGCGTTCATAACCGGGCATCTGAGCTTGGGCCTAATTGCTTTTTTGGGACTGGGGAGGTCGGAAGAAGGGAAAGGAACTGGTTTGCTGTGACTTCATGGAACCTTGGGACGAAAACTGGCACTGAGAAAAATTATGAATTATGTGCCGAATTCTATAGACTGGCATCAGAGTTTTATTGTCTTCAGGTTGATGGGCAGGTAGGAGAAAACATGGTCTGCAAAGCACTAATATTGACTGTATCTGCAATTATAGCTTCAGAGAATCAGAAGAAGATCACATTGCCCGAAAGTGAGGTGAAACAAGCTGTACAGCTTCTAGATAGAGCAGGGAAGATACTGAAGTCAACCTTACCAGGGAATCGGCTTAGTGGTGATCCAGTTGCCACCATGGAACCTGACCTATACTTCATCTACACCTTTTGTGCCTATGACATACACGGAAGGCTCAATGATTCAGGATTACAGCTACAACTTGTAAAGGCTTTTGCTAGTTCAAAGGCCTGCAATCCCAAGTTCCTCCTTCAGATTGGCCTGACTGCTTCCCAAGGTACACAGTGCAATCACGAAGTAGCAACCTTTGCTCTAAATGAATGCCTCTCAGCTTTTCTCTCTTCCTGCTCTCCGGACTACCAAAATGTGGCTCTGATTGTCCGGAAGCTCATTGCGGTGACCAGCATTCACAAAGGCGATACAGATGACGATGCTGTGTATAACATGTACAAGCAAGCTTACAGAATAATGGTGGGACTGAAGGACAGTGTGTACCCCACTGAAGAGGGAAAATGGCTAGCCATGACGGCATGGAATAGGGCGTCAGTGCCTGTGAGACTTGGCCAGATTGATGCGGCAAGGAAATGGATGGATCTCGGTATGCAGCTAGCTAAACATGTTAGTGGAATGGAGACTTATAGGGCATGCATGGAGGACTTCATTAACGGCTTTGAGAAGAAATTTGGTGCCCCAAATGACGGCGGAAACAGACCTCAGCTGGTACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007140 GO:0007143 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0032501 GO:0032504 GO:0034641 GO:0035822 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048232 GO:0048285 GO:0048609 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051704 GO:0061982 GO:0070192 GO:0071139 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPO22

Protein Analysis

945

Amino Acids

104.09

Weight (kDa)

7.53

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPO22	PF08631	193 - 465	8e-36	Meiosis protein SPO22/ZIP4 like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016695)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48390
fragaria_vesca	FvH4_2g17460
malus_domestica	MD05G1153500.v1.1
prunus_persica	Prupe.8G186900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0282191
rosa_laevigata	RLG00000012903
rosa_multiflora	Rmu_sc0014549.1_g000008
rosa_roxburghii	Rroxscaffold_7G00186410
rosa_rugosa	Rorug06G0146100
rosa_samantha	Rh6AG259200 Rh6BG262500 Rh6CG261400 Rh6DG253500
rosa_wichuraiana	Rw6G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	920
Acc16I	TGCGCA	1 cut(s)	1772
Acc36I	ACCTGC	3 cut(s)	920, 1628, 2023
AccB1I	GGYRCC	2 cut(s)	1143, 2792
AccB7I	CCANNNNNTGG	1 cut(s)	2473
AccBSI	CCGCTC	2 cut(s)	294, 1103
AccII	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
AccIII	TCCGGA	2 cut(s)	2458, 2487
AclWI	GGATC	4 cut(s)	825, 1219, 2204, 2706
AcoI	YGGCCR	3 cut(s)	858, 1086, 2668
AcsI	RAATTY	5 cut(s)	452, 1425, 1711, 1985, 2786
AcuI	CTGAAG	8 cut(s)	1429, 1524, 2003, 2076, 2195, 2342, 2606, 2628
AcyI	GRCGYC	3 cut(s)	429, 528, 2648
AfaI	GTAC	8 cut(s)	683, 992, 1556, 2145, 2386, 2555, 2600, 2829
AfeI	AGCGCT	1 cut(s)	703
AfiI	CCNNNNNNNGG	5 cut(s)	168, 1588, 2190, 2473, 2804
AflII	CTTAAG	1 cut(s)	1823
AflIII	ACRYGT	2 cut(s)	2550, 2722
AgsI	TTSAA	5 cut(s)	745, 1354, 1526, 1817, 2331
AjiI	CACGTC	1 cut(s)	1150
AjnI	CCWGG	5 cut(s)	581, 1581, 1732, 1801, 2188
AjuI	GAANNNNNNNTTGG	2 cut(s)	959, 991
Alw21I	GWGCWC	6 cut(s)	33, 324, 660, 771, 1014, 1684
Alw26I	GTCTC	7 cut(s)	526, 803, 912, 1100, 1463, 2656, 2732
AlwI	GGATC	4 cut(s)	825, 1219, 2204, 2706
AlwNI	CAGNNNCTG	2 cut(s)	82, 2657
Ama87I	CYCGRG	1 cut(s)	1043
Aor13HI	TCCGGA	2 cut(s)	2458, 2487
Aor51HI	AGCGCT	1 cut(s)	703
AoxI	GGCC	9 cut(s)	627, 858, 999, 1086, 1656, 1865, 2334, 2365, 2668
ApeKI	GCWGC	9 cut(s)	616, 784, 844, 1132, 1286, 1289, 1334, 1700, 2710
ApoI	RAATTY	5 cut(s)	452, 1425, 1711, 1985, 2786
ArsI	GACNNNNNNTTYG	2 cut(s)	341, 373
AseI	ATTAAT	1 cut(s)	1596
Asp700I	GAANNNNTTC	1 cut(s)	881
AspLEI	GCGC	8 cut(s)	616, 704, 789, 804, 1157, 1163, 1773, 1839
AspS9I	GGNCC	4 cut(s)	595, 627, 1657, 1865
AsuC2I	CCSGG	4 cut(s)	392, 626, 1090, 1850
AsuHPI	GGTGA	7 cut(s)	380, 944, 967, 1292, 2143, 2219, 2515
AsuNHI	GCTAGC	2 cut(s)	2623, 2713
AvaI	CYCGRG	1 cut(s)	1043
AvaII	GGWCC	1 cut(s)	595
BaeGI	GKGCMC	1 cut(s)	2797
BaeI	ACNNNNGTAYC	2 cut(s)	2162, 2195
BalI	TGGCCA	1 cut(s)	2670
BanI	GGYRCC	2 cut(s)	1143, 2792
BanII	GRGCYC	3 cut(s)	33, 771, 1014
BauI	CACGAG	1 cut(s)	440
BbsI	GAAGAC	2 cut(s)	845, 2009
Bbv12I	GWGCWC	6 cut(s)	33, 324, 660, 771, 1014, 1684
BbvCI	CCTCAGC	1 cut(s)	2820
BbvI	GCAGC	9 cut(s)	603, 771, 831, 1119, 1273, 1276, 1321, 1687, 2722
BccI	CCATC	4 cut(s)	952, 1580, 2021, 2689
BcgI	CGANNNNNNTGC	4 cut(s)	428, 462, 826, 860
BciT130I	CCWGG	5 cut(s)	583, 1583, 1734, 1803, 2190
BclI	TGATCA	4 cut(s)	894, 934, 1339, 1486
BcnI	CCSGG	4 cut(s)	392, 626, 1090, 1850
BcoDI	GTCTC	7 cut(s)	526, 803, 912, 1100, 1463, 2656, 2732
BfaI	CTAG	7 cut(s)	567, 671, 945, 2153, 2325, 2624, 2714
BfmI	CTRYAG	2 cut(s)	1568, 1990
BfoI	RGCGCY	3 cut(s)	617, 705, 1158
BfrI	CTTAAG	1 cut(s)	1823
BfuAI	ACCTGC	3 cut(s)	920, 1628, 2023
BglI	GCCNNNNNGGC	3 cut(s)	1094, 1163, 2633
BmcAI	AGTACT	1 cut(s)	683
Bme1390I	CCNGG	9 cut(s)	392, 583, 626, 1090, 1583, 1734, 1803, 1850, 2190
Bme18I	GGWCC	1 cut(s)	595
BmeT110I	CYCGRG	1 cut(s)	1043
BmgBI	CACGTC	1 cut(s)	1150
BmgT120I	GGNCC	4 cut(s)	595, 627, 1657, 1865
BmiI	GGNNCC	4 cut(s)	1145, 1937, 2229, 2794
BmrFI	CCNGG	9 cut(s)	392, 583, 626, 1090, 1583, 1734, 1803, 1850, 2190
BmrI	ACTGGG	1 cut(s)	1896
BmsI	GCATC	5 cut(s)	570, 1862, 2009, 2527, 2668
BmtI	GCTAGC	2 cut(s)	2627, 2717
BmuI	ACTGGG	1 cut(s)	1896
BoxI	GACNNNNGTC	1 cut(s)	428
BpiI	GAAGAC	2 cut(s)	845, 2009
BplI	GAGNNNNNCTC	6 cut(s)	110, 142, 710, 742, 712, 744
BpmI	CTGGAG	2 cut(s)	785, 1119
Bpu10I	CCTNAGC	3 cut(s)	1013, 1227, 2820
BpuEI	CTTGAG	4 cut(s)	203, 806, 1479, 1779
BpuMI	CCSGG	4 cut(s)	392, 626, 1090, 1850
BsaBI	GATNNNNATC	1 cut(s)	509
BsaHI	GRCGYC	3 cut(s)	429, 528, 2648
BsaWI	WCCGGW	7 cut(s)	145, 597, 952, 1277, 1347, 2458, 2487
Bsc4I	CCNNNNNNNGG	5 cut(s)	168, 1588, 2190, 2473, 2804
Bse118I	RCCGGY	3 cut(s)	365, 425, 530
Bse1I	ACTGG	6 cut(s)	675, 1891, 1919, 1957, 2001, 2213
Bse3DI	GCAATG	6 cut(s)	1314, 1398, 1533, 1617, 2114, 2496
Bse8I	GATNNNNATC	1 cut(s)	509
BseAI	TCCGGA	2 cut(s)	2458, 2487
BseBI	CCWGG	5 cut(s)	583, 1583, 1734, 1803, 2190
BseGI	GGATG	2 cut(s)	1591, 2700
BseJI	GATNNNNATC	1 cut(s)	509
BseLI	CCNNNNNNNGG	5 cut(s)	168, 1588, 2190, 2473, 2804
BseMI	GCAATG	6 cut(s)	1314, 1398, 1533, 1617, 2114, 2496
BseMII	CTCAG	9 cut(s)	117, 123, 179, 253, 1027, 1848, 1950, 2448, 2834
BseNI	ACTGG	6 cut(s)	675, 1891, 1919, 1957, 2001, 2213
BseSI	GKGCMC	1 cut(s)	2797
BseX3I	CGGCCG	2 cut(s)	858, 1086
BseXI	GCAGC	9 cut(s)	603, 771, 831, 1119, 1273, 1276, 1321, 1687, 2722
BsgI	GTGCAG	2 cut(s)	1392, 1686
Bsh1236I	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
Bsh1285I	CGRYCG	2 cut(s)	861, 1089
BshFI	GGCC	9 cut(s)	629, 860, 1001, 1088, 1658, 1867, 2336, 2367, 2670
BshNI	GGYRCC	2 cut(s)	1143, 2792
BsiEI	CGRYCG	2 cut(s)	861, 1089
BsiHKAI	GWGCWC	6 cut(s)	33, 324, 660, 771, 1014, 1684
BsiHKCI	CYCGRG	1 cut(s)	1043
BslFI	GGGAC	4 cut(s)	1105, 1897, 1957, 2595
BslI	CCNNNNNNNGG	5 cut(s)	168, 1588, 2190, 2473, 2804
BsmAI	GTCTC	7 cut(s)	526, 803, 912, 1100, 1463, 2656, 2732
BsmBI	CGTCTC	3 cut(s)	803, 912, 1100
BsmFI	GGGAC	4 cut(s)	1105, 1897, 1957, 2595
BsmI	GAATGC	3 cut(s)	1038, 2432, 2511
BsnI	GGCC	9 cut(s)	629, 860, 1001, 1088, 1658, 1867, 2336, 2367, 2670
BsoBI	CYCGRG	1 cut(s)	1043
Bsp1286I	GDGCHC	7 cut(s)	33, 324, 660, 771, 1014, 1684, 2797
Bsp13I	TCCGGA	2 cut(s)	2458, 2487
Bsp1407I	TGTACA	2 cut(s)	2143, 2553
Bsp19I	CCATGG	1 cut(s)	2223
BspANI	GGCC	9 cut(s)	629, 860, 1001, 1088, 1658, 1867, 2336, 2367, 2670
BspCNI	CTCAG	9 cut(s)	116, 124, 180, 252, 1026, 1849, 1951, 2447, 2833
BspEI	TCCGGA	2 cut(s)	2458, 2487
BspFNI	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
BspHI	TCATGA	1 cut(s)	750
BspLI	GGNNCC	4 cut(s)	1145, 1937, 2229, 2794
BspMAI	CTGCAG	1 cut(s)	1572
BspMI	ACCTGC	3 cut(s)	920, 1628, 2023
BspOI	GCTAGC	2 cut(s)	2627, 2717
BspPI	GGATC	4 cut(s)	825, 1219, 2204, 2706
BspQI	GCTCTTC	1 cut(s)	312
BspT107I	GGYRCC	2 cut(s)	1143, 2792
BspTI	CTTAAG	1 cut(s)	1823
BsrBI	CCGCTC	2 cut(s)	294, 1103
BsrDI	GCAATG	6 cut(s)	1314, 1398, 1533, 1617, 2114, 2496
BsrFI	RCCGGY	3 cut(s)	365, 425, 530
BsrGI	TGTACA	2 cut(s)	2143, 2553
BsrI	ACTGG	6 cut(s)	675, 1891, 1919, 1957, 2001, 2213
BssAI	RCCGGY	3 cut(s)	365, 425, 530
BssNI	GRCGYC	3 cut(s)	429, 528, 2648
BssSI	CACGAG	1 cut(s)	440
BssT1I	CCWWGG	5 cut(s)	1437, 1776, 1939, 2223, 2380
Bst2BI	CACGAG	1 cut(s)	440
Bst2UI	CCWGG	5 cut(s)	583, 1583, 1734, 1803, 2190
Bst4CI	ACNGT	4 cut(s)	520, 2074, 2391, 2594
Bst6I	CTCTTC	6 cut(s)	312, 362, 801, 1277, 2452, 2604
BstACI	GRCGYC	3 cut(s)	429, 528, 2648
BstAFI	CTTAAG	1 cut(s)	1823
BstAPI	GCANNNNNTGC	3 cut(s)	1313, 1318, 2414
BstAUI	TGTACA	2 cut(s)	2143, 2553
BstDSI	CCRYGG	2 cut(s)	609, 2223
BstEII	GGTNACC	1 cut(s)	2503
BstF5I	GGATG	2 cut(s)	1591, 2700
BstFNI	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
BstH2I	RGCGCY	3 cut(s)	617, 705, 1158
BstHHI	GCGC	8 cut(s)	616, 704, 789, 804, 1157, 1163, 1773, 1839
BstMAI	GTCTC	7 cut(s)	526, 803, 912, 1100, 1463, 2656, 2732
BstMCI	CGRYCG	2 cut(s)	861, 1089
BstNI	CCWGG	5 cut(s)	583, 1583, 1734, 1803, 2190
BstNSI	RCATGY	4 cut(s)	1316, 2554, 2726, 2754
BstPAI	GACNNNNGTC	1 cut(s)	428
BstPI	GGTNACC	1 cut(s)	2503
BstSCI	CCNGG	9 cut(s)	390, 581, 624, 1088, 1581, 1732, 1801, 1848, 2188
BstSFI	CTRYAG	2 cut(s)	1568, 1990
BstSLI	GKGCMC	1 cut(s)	2797
BstUI	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
BstV1I	GCAGC	9 cut(s)	603, 771, 831, 1119, 1273, 1276, 1321, 1687, 2722
BstV2I	GAAGAC	2 cut(s)	845, 2009
BstX2I	RGATCY	2 cut(s)	817, 2698
BstYI	RGATCY	2 cut(s)	817, 2698
BstZI	CGGCCG	2 cut(s)	858, 1086
BsuRI	GGCC	9 cut(s)	629, 860, 1001, 1088, 1658, 1867, 2336, 2367, 2670
BtgI	CCRYGG	2 cut(s)	609, 2223
BtgZI	GCGATG	3 cut(s)	325, 547, 977
BtrI	CACGTC	1 cut(s)	1150
BtsCI	GGATG	2 cut(s)	1591, 2700
BtsI	GCAGTG	1 cut(s)	1210
BtsIMutI	CAGTG	6 cut(s)	1210, 1956, 2396, 2599, 2604, 2658
BveI	ACCTGC	3 cut(s)	920, 1628, 2023
CaiI	CAGNNNCTG	2 cut(s)	82, 2657
CciI	TCATGA	1 cut(s)	750
CfoI	GCGC	8 cut(s)	616, 704, 789, 804, 1157, 1163, 1773, 1839
Cfr10I	RCCGGY	3 cut(s)	365, 425, 530
Cfr13I	GGNCC	4 cut(s)	595, 627, 1657, 1865
CpoI	CGGWCCG	1 cut(s)	595
CseI	GACGC	7 cut(s)	253, 418, 536, 808, 850, 935, 2637
Csp6I	GTAC	8 cut(s)	682, 991, 1555, 2144, 2385, 2554, 2599, 2828
CspI	CGGWCCG	1 cut(s)	595
CviQI	GTAC	8 cut(s)	682, 991, 1555, 2144, 2385, 2554, 2599, 2828
EaeI	YGGCCR	3 cut(s)	858, 1086, 2668
EagI	CGGCCG	2 cut(s)	858, 1086
Eam1104I	CTCTTC	6 cut(s)	312, 362, 801, 1277, 2452, 2604
EarI	CTCTTC	6 cut(s)	312, 362, 801, 1277, 2452, 2604
EciI	GGCGGA	6 cut(s)	43, 148, 323, 394, 830, 2823
Ecl136II	GAGCTC	3 cut(s)	31, 769, 1012
EclXI	CGGCCG	2 cut(s)	858, 1086
Eco130I	CCWWGG	5 cut(s)	1437, 1776, 1939, 2223, 2380
Eco147I	AGGCCT	1 cut(s)	2336
Eco24I	GRGCYC	3 cut(s)	33, 771, 1014
Eco47I	GGWCC	1 cut(s)	595
Eco47III	AGCGCT	1 cut(s)	703
Eco52I	CGGCCG	2 cut(s)	858, 1086
Eco53kI	GAGCTC	3 cut(s)	31, 769, 1012
Eco57I	CTGAAG	8 cut(s)	1429, 1524, 2003, 2076, 2195, 2342, 2606, 2628
Eco88I	CYCGRG	1 cut(s)	1043
Eco91I	GGTNACC	1 cut(s)	2503
EcoICRI	GAGCTC	3 cut(s)	31, 769, 1012
EcoO65I	GGTNACC	1 cut(s)	2503
EcoRI	GAATTC	2 cut(s)	1425, 1985
EcoRII	CCWGG	5 cut(s)	581, 1581, 1732, 1801, 2188
EcoT14I	CCWWGG	5 cut(s)	1437, 1776, 1939, 2223, 2380
EcoT22I	ATGCAT	2 cut(s)	1314, 2756
EcoT38I	GRGCYC	3 cut(s)	33, 771, 1014
ErhI	CCWWGG	5 cut(s)	1437, 1776, 1939, 2223, 2380
Esp3I	CGTCTC	3 cut(s)	803, 912, 1100
FalI	AAGNNNNNCTT	2 cut(s)	2168, 2200
FaqI	GGGAC	4 cut(s)	1105, 1897, 1957, 2595
FbaI	TGATCA	4 cut(s)	894, 934, 1339, 1486
FokI	GGATG	2 cut(s)	1598, 2707
FriOI	GRGCYC	3 cut(s)	33, 771, 1014
FspBI	CTAG	7 cut(s)	567, 671, 945, 2153, 2325, 2624, 2714
FspI	TGCGCA	1 cut(s)	1772
GlaI	GCGC	8 cut(s)	615, 703, 788, 803, 1156, 1162, 1772, 1838
GsuI	CTGGAG	2 cut(s)	785, 1119
HaeII	RGCGCY	3 cut(s)	617, 705, 1158
HaeIII	GGCC	9 cut(s)	629, 860, 1001, 1088, 1658, 1867, 2336, 2367, 2670
HgaI	GACGC	7 cut(s)	253, 418, 536, 808, 850, 935, 2637
HhaI	GCGC	8 cut(s)	616, 704, 789, 804, 1157, 1163, 1773, 1839
Hin1I	GRCGYC	3 cut(s)	429, 528, 2648
Hin6I	GCGC	8 cut(s)	614, 702, 787, 802, 1155, 1161, 1771, 1837
HinP1I	GCGC	8 cut(s)	614, 702, 787, 802, 1155, 1161, 1771, 1837
HincII	GTYRAC	3 cut(s)	21, 109, 2181
HindII	GTYRAC	3 cut(s)	21, 109, 2181
HindIII	AAGCTT	2 cut(s)	1520, 2562
HphI	GGTGA	7 cut(s)	380, 944, 967, 1292, 2143, 2219, 2515
Hpy166II	GTNNAC	5 cut(s)	21, 109, 2181, 2387, 2599
Hpy8I	GTNNAC	5 cut(s)	21, 109, 2181, 2387, 2599
Hpy99I	CGWCG	6 cut(s)	269, 365, 472, 530, 691, 929
HpyCH4III	ACNGT	4 cut(s)	520, 2074, 2391, 2594
HpyCH4IV	ACGT	2 cut(s)	372, 1149
HpySE526I	ACGT	2 cut(s)	372, 1149
Hsp92I	GRCGYC	3 cut(s)	429, 528, 2648
HspAI	GCGC	8 cut(s)	614, 702, 787, 802, 1155, 1161, 1771, 1837
Kpn2I	TCCGGA	2 cut(s)	2458, 2487
KroI	GCCGGC	1 cut(s)	530
KroNI	GCCGGC	1 cut(s)	532
Ksp22I	TGATCA	4 cut(s)	894, 934, 1339, 1486
LguI	GCTCTTC	1 cut(s)	312
Lsp1109I	GCAGC	9 cut(s)	603, 771, 831, 1119, 1273, 1276, 1321, 1687, 2722
LweI	GCATC	5 cut(s)	570, 1862, 2009, 2527, 2668
MaeI	CTAG	7 cut(s)	567, 671, 945, 2153, 2325, 2624, 2714
MaeII	ACGT	2 cut(s)	372, 1149
MaeIII	GTNAC	6 cut(s)	242, 368, 1363, 1780, 1924, 2503
MbiI	CCGCTC	2 cut(s)	294, 1103
MflI	RGATCY	2 cut(s)	817, 2698
MhlI	GDGCHC	7 cut(s)	33, 324, 660, 771, 1014, 1684, 2797
MlsI	TGGCCA	1 cut(s)	2670
MluNI	TGGCCA	1 cut(s)	2670
MlyI	GAGTC	4 cut(s)	44, 107, 175, 1091
MmeI	TCCRAC	3 cut(s)	300, 549, 1876
Mox20I	TGGCCA	1 cut(s)	2670
Mph1103I	ATGCAT	2 cut(s)	1314, 2756
MreI	CGCCGGCG	1 cut(s)	530
MroI	TCCGGA	2 cut(s)	2458, 2487
MroNI	GCCGGC	1 cut(s)	530
MroXI	GAANNNNTTC	1 cut(s)	881
MscI	TGGCCA	1 cut(s)	2670
MseI	TTAA	5 cut(s)	830, 1596, 1824, 1833, 2769
MslI	CAYNNNNRTG	2 cut(s)	2501, 2727
Msp20I	TGGCCA	1 cut(s)	2670
MspA1I	CMGCKG	1 cut(s)	2824
MspCI	CTTAAG	1 cut(s)	1823
MspR9I	CCNGG	9 cut(s)	392, 583, 626, 1090, 1583, 1734, 1803, 1850, 2190
Mva1269I	GAATGC	3 cut(s)	1038, 2432, 2511
MvaI	CCWGG	5 cut(s)	583, 1583, 1734, 1803, 2190
MvnI	CGCG	5 cut(s)	789, 802, 804, 1163, 1839
NaeI	GCCGGC	1 cut(s)	532
NciI	CCSGG	4 cut(s)	392, 626, 1090, 1850
NcoI	CCATGG	1 cut(s)	2223
NgoMIV	GCCGGC	1 cut(s)	530
NheI	GCTAGC	2 cut(s)	2623, 2713
NlaIV	GGNNCC	4 cut(s)	1145, 1937, 2229, 2794
NmeAIII	GCCGAG	3 cut(s)	37, 836, 1027
NmuCI	GTSAC	4 cut(s)	368, 1780, 1924, 2503
NsbI	TGCGCA	1 cut(s)	1772
NsiI	ATGCAT	2 cut(s)	1314, 2756
NspI	RCATGY	4 cut(s)	1316, 2554, 2726, 2754
PaeI	GCATGC	2 cut(s)	1316, 2754
PagI	TCATGA	1 cut(s)	750
PaqCI	CACCTGC	1 cut(s)	920
PasI	CCCWGGG	1 cut(s)	1733
PceI	AGGCCT	1 cut(s)	2336
PciI	ACATGT	2 cut(s)	2550, 2722
PciSI	GCTCTTC	1 cut(s)	312
PcsI	WCGNNNNNNNCGW	6 cut(s)	264, 369, 378, 999, 1041, 1429
PctI	GAATGC	3 cut(s)	1038, 2432, 2511
PdiI	GCCGGC	1 cut(s)	532
PdmI	GAANNNNTTC	1 cut(s)	881
PfeI	GAWTC	9 cut(s)	6, 136, 510, 601, 1420, 1603, 2098, 2194, 2288
PflFI	GACNNNGTC	1 cut(s)	518
PflMI	CCANNNNNTGG	1 cut(s)	2473
PleI	GAGTC	4 cut(s)	44, 106, 175, 1090
PpsI	GAGTC	4 cut(s)	44, 106, 175, 1090
PscI	ACATGT	2 cut(s)	2550, 2722
PshAI	GACNNNNGTC	1 cut(s)	428
PshBI	ATTAAT	1 cut(s)	1596
Psp124BI	GAGCTC	3 cut(s)	33, 771, 1014
Psp6I	CCWGG	5 cut(s)	581, 1581, 1732, 1801, 2188
PspEI	GGTNACC	1 cut(s)	2503
PspGI	CCWGG	5 cut(s)	581, 1581, 1732, 1801, 2188
PspN4I	GGNNCC	4 cut(s)	1145, 1937, 2229, 2794
PspPI	GGNCC	4 cut(s)	595, 627, 1657, 1865
PsrI	GAACNNNNNNTAC	2 cut(s)	674, 706
PstI	CTGCAG	1 cut(s)	1572
PstNI	CAGNNNCTG	2 cut(s)	82, 2657
PsuI	RGATCY	2 cut(s)	817, 2698
PsyI	GACNNNGTC	1 cut(s)	518
PvuII	CAGCTG	1 cut(s)	2824
RsaI	GTAC	8 cut(s)	683, 992, 1556, 2145, 2386, 2555, 2600, 2829
RsaNI	GTAC	8 cut(s)	682, 991, 1555, 2144, 2385, 2554, 2599, 2828
RseI	CAYNNNNRTG	2 cut(s)	2501, 2727
Rsr2I	CGGWCCG	1 cut(s)	595
RsrII	CGGWCCG	1 cut(s)	595
SacI	GAGCTC	3 cut(s)	33, 771, 1014
SapI	GCTCTTC	1 cut(s)	312
SaqAI	TTAA	5 cut(s)	830, 1596, 1824, 1833, 2769
Sau96I	GGNCC	4 cut(s)	595, 627, 1657, 1865
ScaI	AGTACT	1 cut(s)	683
SchI	GAGTC	4 cut(s)	44, 107, 175, 1091
ScrFI	CCNGG	9 cut(s)	392, 583, 626, 1090, 1583, 1734, 1803, 1850, 2190
SduI	GDGCHC	7 cut(s)	33, 324, 660, 771, 1014, 1684, 2797
SfaNI	GCATC	5 cut(s)	570, 1862, 2009, 2527, 2668
SfcI	CTRYAG	2 cut(s)	1568, 1990
SgrAI	CRCCGGYG	2 cut(s)	365, 530
SinI	GGWCC	1 cut(s)	595
SmiMI	CAYNNNNRTG	2 cut(s)	2501, 2727
SmlI	CTYRAG	5 cut(s)	218, 821, 1494, 1794, 1823
SmoI	CTYRAG	5 cut(s)	218, 821, 1494, 1794, 1823
SphI	GCATGC	2 cut(s)	1316, 2754
SseBI	AGGCCT	1 cut(s)	2336
SspI	AATATT	1 cut(s)	2067
SspMI	CTAG	7 cut(s)	567, 671, 945, 2153, 2325, 2624, 2714
SstI	GAGCTC	3 cut(s)	33, 771, 1014
StuI	AGGCCT	1 cut(s)	2336
StyD4I	CCNGG	9 cut(s)	390, 581, 624, 1088, 1581, 1732, 1801, 1848, 2188
StyI	CCWWGG	5 cut(s)	1437, 1776, 1939, 2223, 2380
TaaI	ACNGT	4 cut(s)	520, 2074, 2391, 2594
TaiI	ACGT	2 cut(s)	375, 1152
TatI	WGTACW	4 cut(s)	681, 1554, 2143, 2553
TauI	GCSGC	4 cut(s)	347, 1166, 1658, 2684
TfiI	GAWTC	9 cut(s)	6, 136, 510, 601, 1420, 1603, 2098, 2194, 2288
Tru1I	TTAA	5 cut(s)	830, 1596, 1824, 1833, 2769
Tru9I	TTAA	5 cut(s)	830, 1596, 1824, 1833, 2769
TscAI	CASTG	6 cut(s)	1210, 1963, 2396, 2599, 2611, 2658
TseFI	GTSAC	4 cut(s)	368, 1780, 1924, 2503
TseI	GCWGC	9 cut(s)	616, 784, 844, 1132, 1286, 1289, 1334, 1700, 2710
Tsp45I	GTSAC	4 cut(s)	368, 1780, 1924, 2503
TspGWI	ACGGA	2 cut(s)	192, 2289
TspRI	CASTG	6 cut(s)	1210, 1963, 2396, 2599, 2611, 2658
Tth111I	GACNNNGTC	1 cut(s)	518
Van91I	CCANNNNNTGG	1 cut(s)	2473
Vha464I	CTTAAG	1 cut(s)	1823
VpaK11BI	GGWCC	1 cut(s)	595
VspI	ATTAAT	1 cut(s)	1596
XapI	RAATTY	5 cut(s)	452, 1425, 1711, 1985, 2786
XbaI	TCTAGA	1 cut(s)	2152
XceI	RCATGY	4 cut(s)	1316, 2554, 2726, 2754
XmnI	GAANNNNTTC	1 cut(s)	881
XspI	CTAG	7 cut(s)	567, 671, 945, 2153, 2325, 2624, 2714
ZrmI	AGTACT	1 cut(s)	683
Zsp2I	ATGCAT	2 cut(s)	1314, 2756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.