Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0016695

Physical Location & Seq

Genomic Coordinates Forward (+)

45522171 .. 45526710

Gene Structure 4540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ25312

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGAGGATTGCCGAGATATCAACGCCGGAGCTCCGGCAAAGCCACAACGACTCCCCCTCCCAATCGCAACCGCATCAGCATCAGCATCTGATCTCCCAGATCGAATCCTCAATCAAGCAAACCGAGAACCTCTCCCCTGAAAACCTCTTACCTGACACAGTTTCCGGCGATCTCCGGCGAGCCTTGACTCAATTGAGCCACCACGCTCCATTCCCCAACTCTCTGAAGCTCGTGATCTGGAAACTCAGTTACCGCCTCTGGAACGCCTGCGTCGATCTCTCCAACACCACCTCCCTCCGCTCCCTCCCTTCCTCCAAAGCCGAAGAGCACGCCAAGCTCCGCCACATCGCCGCCGACCTACTCTGCGTCGCTGGTGAAGTGTCCGGAGTTCCTTCCCCGGCAATCAAGTCTGCCTCCTTCTACCACAAGACCGGAGTCAAATGGCACGAGCTCCGCAAATTCGACCTCGCTTCGTCGTGCTTCGAGAAAGCGACCGATCTCCTCTCGAAGATCGAAATCAGCACGGTCTCCGACTCCGAAGAGAAGAAGCTATTTCTGGATTTGAGTATCGCCAGGTCAAGAACCGCCTGGGAAGTATCGGACCGGAAGCTCGCGGTGGCGCTGCTGAACCGGGCCAAGAACTTGCTATTCGGGTCGCCGGATCACTACAAAACCCTAGCCAATCAGTACTCGATGTTCGGAAAGAGCGCGCTGTCCAATAGCGAGAGTATTTCTCTGAACGAAGCATTGAAGCTCATGAACGAGGCTCTGGAGCTATACGAGAACGGATTGCGCGCGGCGAGGACACGCGACGAGACGGTGGATCTCAAGACGTTAAGGTCGAAGACGCTCCGGTTCATCTCGGCCGTTCATTTGCAGATGAACGAGTTCGAGAGCGTGATCAAATGCGTGAGGGTGTTGAGAGAGGGAGACGCAGGTGATCAGCACCCTAGCCTTCCGGTGATGGCGATGAAGGCCTGGTTGGGGCTGGGGAAGTACGGCGAGGCCGAGAAGGAGCTCAGGGGCATGGTTGTAAATGAAGGCATTCCTGAGGGCGCTTGGGTGTCGGCGGTGGAGGCCTACTTTGAGTCGGCCGGGACGGCTGGAGCAGAGACGGCGAAGGGAGTATTTTTGGGGCTGCTTGGGAGGTGCCACGTCAGCGCCAGCGCAGCGGTTAGGGTTTCTCACAGGGTTCTTGGGGAGAGTTGCAGCGAGGGATCAAAAGTGAGGGCTAGGGTGGTGTCGGAGCTTGTATCTGATGACAGAGTGGTGGCTATCTTCTCCGGTGAGGCAGCTGCGAAGCAGAGGACTGCAATGCATTCTGTGCTTTGGAATTGTGCTGCTGATCATTTCCGGCTAAAGGATTATGTAACAAGTGCAGACCTGTTTGAGAAAGCAATGCTTTATATTCCATTCGACATAGAGAATCGAATTCTCCGAGCCAAGGGCTTCAGAGTTTTGTGTCTCTGCCACCTAGGTCTTTCCCACCTTGATCAAGCTCAAGAATACATTAACGAGGCTGAAAAGCTTGAACCTAACATAGCTTCAGCTTTCCTAAAGTACAAGATTTATCTTCAGAAGAAAGACCAGGATGGGGCTATTACTCAGATTCAGGCAATGACAACCTGCCTTGATTTTATGCCAGACTTTCTCTCCCTTGCAGCCCATGAAGCTGTTGCCTGCCGTGCTCTTTCCATTGCTGTTGCTGCTCTATCAAGTCTGCTGAACTTTTATGCCCCAGGAAAATCCATGCCAACAAGTGAAGTCGTAGTTCTGCGCACCTTGGTCACAATCCTTACTCAGGAACCTGGGAATGAGCTTGAAGCCCTCAAGTTTGTTAAGCGTGTTCATAATCGGGCATCTGAGCTTGGACCTAATTGCTTTTTTGGGACAGGGGAGGTTGGAAAACGGGAAAGGAATTGGTTTGCTGTGACTTCATGGAACCTTGGGACGAAAACTGGCACTGAGAAAAAGTATGAATTATGTGCCGAATTCTATAGACTGGCATCAGAATTTTATTGTCTTCTGGTTGATGGGCAGGTAGGAGAAAACATGGTCTGCAAAGCACTGATACTGACTATATCTGCAATTATAGCTTCAGAAAATCAGAAGAAGACAACATTGCCTGAAAGTGAAGTGAAACAAGCTGTACAGCTTCTAGATAGAGCAGGGAAGATACTGAAGTCAACCTTACCAGGGAATCAACTTAGCGGTGATCCAGTTACCACCATTGAACCTGACCTATACTTCATCTACACCTTTTGTGCCTATGACATATATGGAAGGCTCAATGATTCAGGATTACAGCTACAACTTGTGAAGACTTTTGCTAGTTCCAAGGCCTGCAATCCCAAGTTCCTCCTTCAGATTGGCCTCACTGCTTCACAAGGTCCACAGTCCAATCATGAAGTAGCGACCTTTGCTCTAAATGAATGCCTCTCAGTTTTCCTCTCTTCCCCCTCTCCGGACTACCAAAATGTGGCTCTGATCGTCCGGAAGCTCATTGCTGTGACCAGCATTCACAAAGGTGATACAGATGATGATGCTGTGTATAACATGTACAAGCAAGCTTACAGAATTATGGTAGGACTGAAGGACAGTGAGTATCCGACTGAAGAAGGAAAATGGCTAGCGATGACTGCATGGAATAGGGCATCAGTGCCTGTGAGGCTTGGCCAGATTGATGCGGCAAGGAAATGGATGGATGTCGGTATGCAGCTAGCTAAGCATGTTAATGGAATGGATACTTATAGGGCGTGCATGGAGGATTTCATTACCAGCTTTGAGAAGAAATTTGGTGCCCCAAATGATGGCGTAAACAGACCTCAGCTAGTAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007140 GO:0007143 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0032501 GO:0032504 GO:0034641 GO:0035822 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048232 GO:0048285 GO:0048609 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051704 GO:0061982 GO:0070192 GO:0071139 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPO22

Protein Analysis

946

Amino Acids

104.79

Weight (kDa)

7.1

Isoelectric Point (pI)

43.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPO22	PF08631	195 - 467	4.8e-36	Meiosis protein SPO22/ZIP4 like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016695)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48390
fragaria_vesca	FvH4_2g17460
malus_domestica	MD05G1153500.v1.1
prunus_persica	Prupe.8G186900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0282191
rosa_laevigata	RLG00000012903
rosa_multiflora	Rmu_sc0014549.1_g000008
rosa_roxburghii	Rroxscaffold_7G00186410
rosa_rugosa	Rorug06G0146100
rosa_samantha	Rh6AG259200 Rh6BG262500 Rh6CG261400 Rh6DG253500
rosa_wichuraiana	Rw6G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	926
AasI	GACNNNNNNGTC	1 cut(s)	838
Acc16I	TGCGCA	1 cut(s)	1778
Acc36I	ACCTGC	3 cut(s)	926, 1634, 2029
AccB1I	GGYRCC	2 cut(s)	1149, 2798
AccB7I	CCANNNNNTGG	2 cut(s)	2479, 2810
AccBSI	CCGCTC	1 cut(s)	300
AccII	CGCG	5 cut(s)	614, 710, 795, 797, 810
AccIII	TCCGGA	3 cut(s)	383, 2464, 2493
AclWI	GGATC	4 cut(s)	669, 831, 1225, 2210
AcoI	YGGCCR	3 cut(s)	864, 1092, 2674
AcsI	RAATTY	5 cut(s)	458, 1431, 1991, 2012, 2792
AcuI	CTGAAG	9 cut(s)	245, 1435, 1530, 1559, 2082, 2201, 2348, 2612, 2634
AfaI	GTAC	5 cut(s)	689, 998, 1562, 2151, 2561
AfiI	CCNNNNNNNGG	9 cut(s)	984, 1360, 1444, 1594, 1801, 2196, 2464, 2479, 2810
AflIII	ACRYGT	1 cut(s)	2556
AgsI	TTSAA	4 cut(s)	751, 1532, 1823, 2234
AjiI	CACGTC	1 cut(s)	1156
AjnI	CCWGG	7 cut(s)	572, 587, 977, 1587, 1738, 1807, 2194
AjuI	GAANNNNNNNTTGG	2 cut(s)	965, 997
AleI	CACNNNNGTG	1 cut(s)	2526
Alw21I	GWGCWC	5 cut(s)	33, 330, 453, 1020, 1690
Alw26I	GTCTC	5 cut(s)	532, 809, 924, 1106, 1469
AlwI	GGATC	4 cut(s)	669, 831, 1225, 2210
AlwNI	CAGNNNCTG	2 cut(s)	88, 2663
Aor13HI	TCCGGA	3 cut(s)	383, 2464, 2493
AoxI	GGCC	9 cut(s)	633, 864, 975, 1005, 1077, 1092, 2340, 2371, 2674
ApoI	RAATTY	5 cut(s)	458, 1431, 1991, 2012, 2792
Asp700I	GAANNNNTTC	2 cut(s)	887, 1044
AspA2I	CCTAGG	1 cut(s)	1474
AspLEI	GCGC	9 cut(s)	622, 710, 712, 795, 797, 1058, 1163, 1169, 1779
AspS9I	GGNCC	4 cut(s)	601, 633, 1871, 2390
AsuC2I	CCSGG	3 cut(s)	398, 632, 1096
AsuHPI	GGTGA	6 cut(s)	386, 950, 973, 1298, 2225, 2540
AsuNHI	GCTAGC	2 cut(s)	2629, 2719
AvaII	GGWCC	3 cut(s)	601, 1871, 2390
AvrII	CCTAGG	1 cut(s)	1474
AxyI	CCTNAGG	1 cut(s)	1050
BaeGI	GKGCMC	1 cut(s)	2803
BaeI	ACNNNNGTAYC	2 cut(s)	2168, 2201
BalI	TGGCCA	1 cut(s)	2676
BanI	GGYRCC	2 cut(s)	1149, 2798
BanII	GRGCYC	3 cut(s)	33, 453, 1020
BauI	CACGAG	2 cut(s)	230, 446
BbsI	GAAGAC	4 cut(s)	851, 2015, 2120, 2327
Bbv12I	GWGCWC	5 cut(s)	33, 330, 453, 1020, 1690
BbvCI	CCTCAGC	1 cut(s)	2826
BccI	CCATC	5 cut(s)	958, 1586, 2027, 2695, 2804
BceAI	ACGGC	5 cut(s)	851, 1015, 1116, 1131, 1668
BciT130I	CCWGG	7 cut(s)	574, 589, 979, 1589, 1740, 1809, 2196
BciVI	GTATCC	2 cut(s)	2616, 2737
BclI	TGATCA	4 cut(s)	900, 940, 1345, 1492
BcnI	CCSGG	3 cut(s)	398, 632, 1096
BcoDI	GTCTC	5 cut(s)	532, 809, 924, 1106, 1469
BfmI	CTRYAG	1 cut(s)	1996
BfoI	RGCGCY	3 cut(s)	623, 1059, 1164
BfuAI	ACCTGC	3 cut(s)	926, 1634, 2029
BfuI	GTATCC	2 cut(s)	2616, 2737
BglI	GCCNNNNNGGC	2 cut(s)	1100, 2668
BlnI	CCTAGG	1 cut(s)	1474
BlpI	GCTNAGC	1 cut(s)	2724
BmcAI	AGTACT	1 cut(s)	689
Bme18I	GGWCC	3 cut(s)	601, 1871, 2390
BmgBI	CACGTC	1 cut(s)	1156
BmgT120I	GGNCC	4 cut(s)	601, 633, 1871, 2390
BmiI	GGNNCC	4 cut(s)	1151, 1806, 1943, 2800
BmsI	GCATC	8 cut(s)	82, 88, 94, 1868, 2015, 2533, 2663, 2674
BmtI	GCTAGC	2 cut(s)	2633, 2723
BoxI	GACNNNNGTC	1 cut(s)	434
BpiI	GAAGAC	4 cut(s)	851, 2015, 2120, 2327
BplI	GAGNNNNNCTC	4 cut(s)	116, 148, 718, 750
BpmI	CTGGAG	2 cut(s)	791, 1125
Bpu10I	CCTNAGC	2 cut(s)	1019, 2826
Bpu1102I	GCTNAGC	1 cut(s)	2724
BpuEI	CTTGAG	3 cut(s)	812, 1485, 1814
BpuMI	CCSGG	3 cut(s)	398, 632, 1096
BsaBI	GATNNNNATC	1 cut(s)	515
BsaI	GGTCTC	1 cut(s)	532
BsaWI	WCCGGW	8 cut(s)	383, 431, 603, 852, 958, 1283, 2464, 2493
BsaXI	ACNNNNNCTCC	8 cut(s)	35, 41, 65, 71, 275, 305, 1637, 1667
Bsc4I	CCNNNNNNNGG	9 cut(s)	984, 1360, 1444, 1594, 1801, 2196, 2464, 2479, 2810
Bse1I	ACTGG	3 cut(s)	1963, 2007, 2219
Bse21I	CCTNAGG	1 cut(s)	1050
Bse3DI	GCAATG	6 cut(s)	1320, 1404, 1623, 1695, 2120, 2502
Bse8I	GATNNNNATC	1 cut(s)	515
BseAI	TCCGGA	3 cut(s)	383, 2464, 2493
BseBI	CCWGG	7 cut(s)	574, 589, 979, 1589, 1740, 1809, 2196
BseGI	GGATG	3 cut(s)	1597, 2706, 2710
BseJI	GATNNNNATC	1 cut(s)	515
BseLI	CCNNNNNNNGG	9 cut(s)	984, 1360, 1444, 1594, 1801, 2196, 2464, 2479, 2810
BseMI	GCAATG	6 cut(s)	1320, 1404, 1623, 1695, 2120, 2502
BseMII	CTCAG	9 cut(s)	259, 1033, 1041, 1619, 1814, 1854, 1956, 2454, 2840
BseNI	ACTGG	3 cut(s)	1963, 2007, 2219
BsePI	GCGCGC	2 cut(s)	708, 793
BseRI	GAGGAG	1 cut(s)	491
BseSI	GKGCMC	1 cut(s)	2803
BseX3I	CGGCCG	2 cut(s)	864, 1092
BseYI	CCCAGC	1 cut(s)	988
BsgI	GTGCAG	1 cut(s)	1398
Bsh1236I	CGCG	5 cut(s)	614, 710, 795, 797, 810
Bsh1285I	CGRYCG	3 cut(s)	495, 867, 1095
BshFI	GGCC	9 cut(s)	635, 866, 977, 1007, 1079, 1094, 2342, 2373, 2676
BshNI	GGYRCC	2 cut(s)	1149, 2798
BsiEI	CGRYCG	3 cut(s)	495, 867, 1095
BsiHKAI	GWGCWC	5 cut(s)	33, 330, 453, 1020, 1690
BslFI	GGGAC	3 cut(s)	1111, 1903, 1963
BslI	CCNNNNNNNGG	9 cut(s)	984, 1360, 1444, 1594, 1801, 2196, 2464, 2479, 2810
BsmAI	GTCTC	5 cut(s)	532, 809, 924, 1106, 1469
BsmBI	CGTCTC	3 cut(s)	809, 924, 1106
BsmFI	GGGAC	3 cut(s)	1111, 1903, 1963
BsmI	GAATGC	4 cut(s)	1044, 1318, 2438, 2517
BsnI	GGCC	9 cut(s)	635, 866, 977, 1007, 1079, 1094, 2342, 2373, 2676
Bso31I	GGTCTC	1 cut(s)	532
Bsp1286I	GDGCHC	6 cut(s)	33, 330, 453, 1020, 1690, 2803
Bsp13I	TCCGGA	3 cut(s)	383, 2464, 2493
Bsp1407I	TGTACA	2 cut(s)	2149, 2559
Bsp1720I	GCTNAGC	1 cut(s)	2724
BspANI	GGCC	9 cut(s)	635, 866, 977, 1007, 1079, 1094, 2342, 2373, 2676
BspCNI	CTCAG	9 cut(s)	258, 1032, 1042, 1618, 1813, 1855, 1957, 2453, 2839
BspEI	TCCGGA	3 cut(s)	383, 2464, 2493
BspFNI	CGCG	5 cut(s)	614, 710, 795, 797, 810
BspHI	TCATGA	2 cut(s)	756, 2404
BspLI	GGNNCC	4 cut(s)	1151, 1806, 1943, 2800
BspMI	ACCTGC	3 cut(s)	926, 1634, 2029
BspOI	GCTAGC	2 cut(s)	2633, 2723
BspPI	GGATC	4 cut(s)	669, 831, 1225, 2210
BspQI	GCTCTTC	1 cut(s)	318
BspT107I	GGYRCC	2 cut(s)	1149, 2798
BspTNI	GGTCTC	1 cut(s)	532
BsrBI	CCGCTC	1 cut(s)	300
BsrDI	GCAATG	6 cut(s)	1320, 1404, 1623, 1695, 2120, 2502
BsrGI	TGTACA	2 cut(s)	2149, 2559
BsrI	ACTGG	3 cut(s)	1963, 2007, 2219
BssHII	GCGCGC	2 cut(s)	708, 793
BssSI	CACGAG	2 cut(s)	230, 446
BssT1I	CCWWGG	5 cut(s)	1443, 1474, 1782, 1945, 2337
Bst2BI	CACGAG	2 cut(s)	230, 446
Bst2UI	CCWGG	7 cut(s)	574, 589, 979, 1589, 1740, 1809, 2196
Bst4CI	ACNGT	5 cut(s)	160, 526, 820, 2397, 2600
Bst6I	CTCTTC	3 cut(s)	318, 534, 2458
BstAPI	GCANNNNNTGC	1 cut(s)	1324
BstAUI	TGTACA	2 cut(s)	2149, 2559
BstENI	CCTNNNNNAGG	1 cut(s)	1799
BstF5I	GGATG	3 cut(s)	1597, 2706, 2710
BstFNI	CGCG	5 cut(s)	614, 710, 795, 797, 810
BstH2I	RGCGCY	3 cut(s)	623, 1059, 1164
BstHHI	GCGC	9 cut(s)	622, 710, 712, 795, 797, 1058, 1163, 1169, 1779
BstMAI	GTCTC	5 cut(s)	532, 809, 924, 1106, 1469
BstMCI	CGRYCG	3 cut(s)	495, 867, 1095
BstNI	CCWGG	7 cut(s)	574, 589, 979, 1589, 1740, 1809, 2196
BstNSI	RCATGY	2 cut(s)	2560, 2732
BstPAI	GACNNNNGTC	1 cut(s)	434
BstSFI	CTRYAG	1 cut(s)	1996
BstSLI	GKGCMC	1 cut(s)	2803
BstUI	CGCG	5 cut(s)	614, 710, 795, 797, 810
BstV2I	GAAGAC	4 cut(s)	851, 2015, 2120, 2327
BstX2I	RGATCY	1 cut(s)	823
BstYI	RGATCY	1 cut(s)	823
BstZI	CGGCCG	2 cut(s)	864, 1092
Bsu36I	CCTNAGG	1 cut(s)	1050
BsuI	GTATCC	2 cut(s)	2616, 2737
BsuRI	GGCC	9 cut(s)	635, 866, 977, 1007, 1079, 1094, 2342, 2373, 2676
BtgZI	GCGATG	3 cut(s)	331, 983, 2648
BtrI	CACGTC	1 cut(s)	1156
BtsCI	GGATG	3 cut(s)	1597, 2706, 2710
BtsI	GCAGTG	1 cut(s)	2376
BtsIMutI	CAGTG	5 cut(s)	1962, 2066, 2376, 2605, 2664
BveI	ACCTGC	3 cut(s)	926, 1634, 2029
CaiI	CAGNNNCTG	2 cut(s)	88, 2663
CciI	TCATGA	2 cut(s)	756, 2404
CfoI	GCGC	9 cut(s)	622, 710, 712, 795, 797, 1058, 1163, 1169, 1779
Cfr13I	GGNCC	4 cut(s)	601, 633, 1871, 2390
CpoI	CGGWCCG	1 cut(s)	601
CseI	GACGC	4 cut(s)	259, 355, 856, 941
Csp6I	GTAC	5 cut(s)	688, 997, 1561, 2150, 2560
CspI	CGGWCCG	1 cut(s)	601
CviQI	GTAC	5 cut(s)	688, 997, 1561, 2150, 2560
DrdI	GACNNNNNNGTC	1 cut(s)	838
DseDI	GACNNNNNNGTC	1 cut(s)	838
EaeI	YGGCCR	3 cut(s)	864, 1092, 2674
EagI	CGGCCG	2 cut(s)	864, 1092
Eam1104I	CTCTTC	3 cut(s)	318, 534, 2458
EarI	CTCTTC	3 cut(s)	318, 534, 2458
EciI	GGCGGA	1 cut(s)	329
Ecl136II	GAGCTC	3 cut(s)	31, 451, 1018
EclXI	CGGCCG	2 cut(s)	864, 1092
Eco130I	CCWWGG	5 cut(s)	1443, 1474, 1782, 1945, 2337
Eco147I	AGGCCT	3 cut(s)	977, 1079, 2342
Eco24I	GRGCYC	3 cut(s)	33, 453, 1020
Eco31I	GGTCTC	1 cut(s)	532
Eco32I	GATATC	1 cut(s)	18
Eco47I	GGWCC	3 cut(s)	601, 1871, 2390
Eco52I	CGGCCG	2 cut(s)	864, 1092
Eco53kI	GAGCTC	3 cut(s)	31, 451, 1018
Eco57I	CTGAAG	9 cut(s)	245, 1435, 1530, 1559, 2082, 2201, 2348, 2612, 2634
Eco81I	CCTNAGG	1 cut(s)	1050
EcoICRI	GAGCTC	3 cut(s)	31, 451, 1018
EcoNI	CCTNNNNNAGG	1 cut(s)	1799
EcoRI	GAATTC	2 cut(s)	1431, 1991
EcoRII	CCWGG	7 cut(s)	572, 587, 977, 1587, 1738, 1807, 2194
EcoRV	GATATC	1 cut(s)	18
EcoT14I	CCWWGG	5 cut(s)	1443, 1474, 1782, 1945, 2337
EcoT22I	ATGCAT	1 cut(s)	1320
EcoT38I	GRGCYC	3 cut(s)	33, 453, 1020
ErhI	CCWWGG	5 cut(s)	1443, 1474, 1782, 1945, 2337
Esp3I	CGTCTC	3 cut(s)	809, 924, 1106
FalI	AAGNNNNNCTT	4 cut(s)	1386, 1418, 2174, 2206
FaqI	GGGAC	3 cut(s)	1111, 1903, 1963
FbaI	TGATCA	4 cut(s)	900, 940, 1345, 1492
FokI	GGATG	3 cut(s)	1604, 2713, 2717
FriOI	GRGCYC	3 cut(s)	33, 453, 1020
FspI	TGCGCA	1 cut(s)	1778
GlaI	GCGC	9 cut(s)	621, 709, 711, 794, 796, 1057, 1162, 1168, 1778
GsaI	CCCAGC	1 cut(s)	992
GsuI	CTGGAG	2 cut(s)	791, 1125
HaeII	RGCGCY	3 cut(s)	623, 1059, 1164
HaeIII	GGCC	9 cut(s)	635, 866, 977, 1007, 1079, 1094, 2342, 2373, 2676
HgaI	GACGC	4 cut(s)	259, 355, 856, 941
HhaI	GCGC	9 cut(s)	622, 710, 712, 795, 797, 1058, 1163, 1169, 1779
Hin6I	GCGC	9 cut(s)	620, 708, 710, 793, 795, 1056, 1161, 1167, 1777
HinP1I	GCGC	9 cut(s)	620, 708, 710, 793, 795, 1056, 1161, 1167, 1777
HincII	GTYRAC	1 cut(s)	2187
HindII	GTYRAC	1 cut(s)	2187
HindIII	AAGCTT	2 cut(s)	1526, 2568
HphI	GGTGA	6 cut(s)	386, 950, 973, 1298, 2225, 2540
Hpy166II	GTNNAC	3 cut(s)	2187, 2393, 2818
Hpy8I	GTNNAC	3 cut(s)	2187, 2393, 2818
Hpy99I	CGWCG	4 cut(s)	275, 371, 478, 815
HpyCH4III	ACNGT	5 cut(s)	160, 526, 820, 2397, 2600
HpyCH4IV	ACGT	2 cut(s)	833, 1155
HpySE526I	ACGT	2 cut(s)	833, 1155
HspAI	GCGC	9 cut(s)	620, 708, 710, 793, 795, 1056, 1161, 1167, 1777
Kpn2I	TCCGGA	3 cut(s)	383, 2464, 2493
Ksp22I	TGATCA	4 cut(s)	900, 940, 1345, 1492
LguI	GCTCTTC	1 cut(s)	318
LweI	GCATC	8 cut(s)	82, 88, 94, 1868, 2015, 2533, 2663, 2674
MaeII	ACGT	2 cut(s)	833, 1155
MaeIII	GTNAC	6 cut(s)	248, 1369, 1786, 1930, 2221, 2509
MbiI	CCGCTC	1 cut(s)	300
MfeI	CAATTG	1 cut(s)	191
MflI	RGATCY	1 cut(s)	823
MhlI	GDGCHC	6 cut(s)	33, 330, 453, 1020, 1690, 2803
MlsI	TGGCCA	1 cut(s)	2676
MluNI	TGGCCA	1 cut(s)	2676
MlyI	GAGTC	5 cut(s)	44, 181, 444, 527, 1097
MmeI	TCCRAC	5 cut(s)	306, 555, 1224, 1882, 2633
Mox20I	TGGCCA	1 cut(s)	2676
Mph1103I	ATGCAT	1 cut(s)	1320
MroI	TCCGGA	3 cut(s)	383, 2464, 2493
MroXI	GAANNNNTTC	2 cut(s)	887, 1044
MscI	TGGCCA	1 cut(s)	2676
MseI	TTAA	4 cut(s)	836, 1512, 1839, 2733
MslI	CAYNNNNRTG	3 cut(s)	2507, 2526, 2733
Msp20I	TGGCCA	1 cut(s)	2676
MspA1I	CMGCKG	2 cut(s)	1172, 1295
MunI	CAATTG	1 cut(s)	191
Mva1269I	GAATGC	4 cut(s)	1044, 1318, 2438, 2517
MvaI	CCWGG	7 cut(s)	574, 589, 979, 1589, 1740, 1809, 2196
MvnI	CGCG	5 cut(s)	614, 710, 795, 797, 810
NciI	CCSGG	3 cut(s)	398, 632, 1096
NheI	GCTAGC	2 cut(s)	2629, 2719
NlaIV	GGNNCC	4 cut(s)	1151, 1806, 1943, 2800
NmeAIII	GCCGAG	3 cut(s)	37, 842, 1033
NmuCI	GTSAC	3 cut(s)	1786, 1930, 2509
NsbI	TGCGCA	1 cut(s)	1778
NsiI	ATGCAT	1 cut(s)	1320
NspI	RCATGY	2 cut(s)	2560, 2732
OliI	CACNNNNGTG	1 cut(s)	2526
PagI	TCATGA	2 cut(s)	756, 2404
PaqCI	CACCTGC	1 cut(s)	926
PauI	GCGCGC	2 cut(s)	708, 793
PceI	AGGCCT	3 cut(s)	977, 1079, 2342
PciI	ACATGT	1 cut(s)	2556
PciSI	GCTCTTC	1 cut(s)	318
PcsI	WCGNNNNNNNCGW	4 cut(s)	270, 839, 1005, 1435
PctI	GAATGC	4 cut(s)	1044, 1318, 2438, 2517
PdmI	GAANNNNTTC	2 cut(s)	887, 1044
PfeI	GAWTC	5 cut(s)	104, 1426, 1609, 2200, 2294
PflMI	CCANNNNNTGG	2 cut(s)	2479, 2810
PleI	GAGTC	5 cut(s)	44, 181, 443, 527, 1096
PpsI	GAGTC	5 cut(s)	44, 181, 443, 527, 1096
PscI	ACATGT	1 cut(s)	2556
PshAI	GACNNNNGTC	1 cut(s)	434
Psp124BI	GAGCTC	3 cut(s)	33, 453, 1020
Psp6I	CCWGG	7 cut(s)	572, 587, 977, 1587, 1738, 1807, 2194
PspFI	CCCAGC	1 cut(s)	988
PspGI	CCWGG	7 cut(s)	572, 587, 977, 1587, 1738, 1807, 2194
PspN4I	GGNNCC	4 cut(s)	1151, 1806, 1943, 2800
PspPI	GGNCC	4 cut(s)	601, 633, 1871, 2390
PsrI	GAACNNNNNNTAC	2 cut(s)	680, 712
PstNI	CAGNNNCTG	2 cut(s)	88, 2663
PsuI	RGATCY	1 cut(s)	823
PteI	GCGCGC	2 cut(s)	708, 793
PvuII	CAGCTG	1 cut(s)	1295
RsaI	GTAC	5 cut(s)	689, 998, 1562, 2151, 2561
RsaNI	GTAC	5 cut(s)	688, 997, 1561, 2150, 2560
RseI	CAYNNNNRTG	3 cut(s)	2507, 2526, 2733
Rsr2I	CGGWCCG	1 cut(s)	601
RsrII	CGGWCCG	1 cut(s)	601
SacI	GAGCTC	3 cut(s)	33, 453, 1020
SapI	GCTCTTC	1 cut(s)	318
SaqAI	TTAA	4 cut(s)	836, 1512, 1839, 2733
Sau96I	GGNCC	4 cut(s)	601, 633, 1871, 2390
ScaI	AGTACT	1 cut(s)	689
SchI	GAGTC	5 cut(s)	44, 181, 444, 527, 1097
SduI	GDGCHC	6 cut(s)	33, 330, 453, 1020, 1690, 2803
SfaNI	GCATC	8 cut(s)	82, 88, 94, 1868, 2015, 2533, 2663, 2674
SfcI	CTRYAG	1 cut(s)	1996
SinI	GGWCC	3 cut(s)	601, 1871, 2390
SmiMI	CAYNNNNRTG	3 cut(s)	2507, 2526, 2733
SmlI	CTYRAG	3 cut(s)	827, 1500, 1829
SmoI	CTYRAG	3 cut(s)	827, 1500, 1829
SseBI	AGGCCT	3 cut(s)	977, 1079, 2342
SstI	GAGCTC	3 cut(s)	33, 453, 1020
StuI	AGGCCT	3 cut(s)	977, 1079, 2342
StyI	CCWWGG	5 cut(s)	1443, 1474, 1782, 1945, 2337
TaaI	ACNGT	5 cut(s)	160, 526, 820, 2397, 2600
TaiI	ACGT	2 cut(s)	836, 1158
TaqII	GACCGA	1 cut(s)	509
TatI	WGTACW	4 cut(s)	687, 1560, 2149, 2559
TauI	GCSGC	3 cut(s)	353, 800, 2690
TfiI	GAWTC	5 cut(s)	104, 1426, 1609, 2200, 2294
Tru1I	TTAA	4 cut(s)	836, 1512, 1839, 2733
Tru9I	TTAA	4 cut(s)	836, 1512, 1839, 2733
TscAI	CASTG	5 cut(s)	1969, 2073, 2383, 2605, 2664
TseFI	GTSAC	3 cut(s)	1786, 1930, 2509
Tsp45I	GTSAC	3 cut(s)	1786, 1930, 2509
TspGWI	ACGGA	1 cut(s)	801
TspRI	CASTG	5 cut(s)	1969, 2073, 2383, 2605, 2664
Van91I	CCANNNNNTGG	2 cut(s)	2479, 2810
VpaK11BI	GGWCC	3 cut(s)	601, 1871, 2390
XagI	CCTNNNNNAGG	1 cut(s)	1799
XapI	RAATTY	5 cut(s)	458, 1431, 1991, 2012, 2792
XbaI	TCTAGA	1 cut(s)	2158
XceI	RCATGY	2 cut(s)	2560, 2732
XmaJI	CCTAGG	1 cut(s)	1474
XmnI	GAANNNNTTC	2 cut(s)	887, 1044
ZrmI	AGTACT	1 cut(s)	689
Zsp2I	ATGCAT	1 cut(s)	1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.