Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016695

Physical Location & Seq

Genomic Coordinates Forward (+)

28434434 .. 28437802

Gene Structure 3369 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1153500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGAGGATCGCCGAGCTCTCAACGCCGGAGCTCCGGCAAGGCCACGCCGATTCCCAACCTCAACACCAACCTCCATCGCAATCTCATCAACTTCTCATCTCCCAAATCGAGTCCTCAATCAAACAAATCGAGAATCTGTCTCCGGAAAAGCTCTCCCCGGACACAGTCTCCGCCGATCTCCGCCGATTCTCGACTAAACTGAGCCAACTCGCTCCCTTCCCCAACTCACTGAAGCTCCTCATCTGGAAGCTCAGTTACCGTCTCTGGAACGCCTGCGTCGACCTTTCAAACGCGGCCTCCCTCCGCTCCCTCAGCGCATCCAGAGCTGAAGATCACGCCAAGCTCCGCCACGTCGCCGCTGACCTCCTCTTCATATCCGGCGACGTCTCTGGTGTCCCTTCCCCGGTGATCAAGTCCGCCTCCTTCTACCTCAAGACCGGCCTCATATGGCACGACCTCCGGAGCTTCGATCTCGCTTCCTCATGCTTCGAGCGAGCTACAGATATAGTCTCGAAGATCGACATCGACAAAGTCTCCGATTGCGGAGAGAGAAAGCTTCTCCTGGACCTGAGCATCGCGAGGTCGAAAACCGCATGGGACGTCTCCGACCGGAATGTCGCGATCGCGCTGCTGAACAGGGCCAAGAGCTTGCTATTCGGGTCGCCGGACCACCACAAGGCGCTCGCGAACCAGTTCTTGGCGTTCAGCAAGACGGCGCTTGCCAAGAGCGAGATTCAGGACCTAAACGACGCGTTGAAGCTGATGAACGAGGCTCTGGATCTGTACGAGAAGGGATTGCGTGTGGCGAGAACACGCGAAGAGATCATGGAACTCAAGGATCTGAGGTCAAAGACGCTGCGATTCATTTCGGCCGTCCATCTGCAGATGAACGAGTTTGAGAGCGTGATCAAGTGCGTGAGGGTGCTGAGAGAGGGGTGCGAGAGTGGGGACCACCATCCGAGCCTATCGGTTCTGGCGATGAAGGGCTGGTTGGGGTTGGGGAAGTACGCCGAGGCCGAGAAGGAGCTCAGGAGCATGGTGGTAAATAAGGGGATTCCGGAGGGCGTTTTTGTGTCGGCTGTGGAGGCTTATTTTCAGGCGGCTGGGACTGCCGGAGCAGAAACGGCGAAGGGAGTGTTTCTGGGACTGTTGGGAAGGTGCCACGTCAGCGCCAGCTCGGCTGTTAGGGTGGCCCACCGGGTGATTGGTGACGCCGGTGAGGGATCAAGAATTCGGGCCAAGGTGGTGGCGGAGCTCGTATCAGACGAGAGAGTGGTGGCCCTCTTCAATGGTGACGCCGCTGCCCAACAGAGAACTGCAATGCATTCAGTGCTTTGGAATTGTGGAGCTGAGCATTTTCTATCGAAAGATTACGAAACTAGTGCAGAAATGTTTGAGAAAGCAATGCTTTATATCCCATTTGACATTGAGAGTAGAATTCTCAGGGCCAAGGGATTTAGAGTTTTGTGTCTCTGCCATTTGGGTCTCTCCCAGCTTGATCAAGCACATGAATACATCAACGAGGCCGAAAAGCTCGAACCGAACATCGCTTCAACTTTCCTGAAGTTCAAGATTTATCTGCAGAAGAAAGACCAGAATGGAGCTACTAATCAGATTCAGGCAATGACAACCTGCTTTGATTTTACACCAGATTTTCTCTCCCTTGCAGCCCATGAAGCTGTTGCTTGCCGTGTTCTTGCTGTTGCGGTTGCTTCTCTTTCAAGTCTGTTGAACTTCTATACCCCGGGAAAATCCATGCCGGCAACTGAAGTTGTGGTTCTACGGACCTTGGTCACAATTCTAACCCAAGAACCAGGGAATGAGGATGAAGCCCTCAAGTTTCTTAAGCGCGTCCATAATCGTGCGTCTGAGCTTGGTCCTGATTCCTTTTTCGGGACAGGGGAGGTCGGAAGACGGGAGAGGAAGTGGTTTGCTGTGACTTCATGGAACTTGGGGACCAAAACCGGGAAGGAGAAGAACTATGAATTGTGCGGCGAATTCTTGAGACTGGCATCGGAGTTTTATGGTCTTCTGGTTGATGGGCAAGTAGAAGAAAACATGGTCTGCAAGTCGTTAATTCTGAGTGTATCAGCAATTATAGCTTCAGAGAATCAGAGGAAGACTACATTGAATGAAAGTGAAGTCAAACAAGCTCAAGAACTTCTAGATAGAGCTGGGAAGATATTGAAAACAACCTCAACCGGGAATCAACTTATCGGTGATCAATTTTCAACAACTGAGCCTGATTTGTTCTTCATCTACACCTTTTGTGCCTATGAGATACATGGAAGGCTCAATGACTCATCATCACAGCTAAAACTAGTGAAGAATTTTGCCACTTCAAAGGCTTGCAATCACAAAAACCTCCTTCAGATCGGCATCAACGCTTCACAATCTCCCCGAACCAATCCTGAAGTCGCAGTCTATGCTCTAAACGAGTGCCTATCGTCCTTCCTCTCTTCCTCCACGGCAGACTACCAAAGCGTGGCTCTCATTGTTCGGAAGCTCATTGGAGTGACCAGCATTCACAAGGGGGATGCAGACGACGATGCTGTTTATGGCATGTACAAGCAGGCTTACAGAGTAATGGTGGGACTGAAGGACGGAGAGTATCCGACGGAAGAAGGGAAATGGCTTGCCATGACGGCATGGAACAGGGCATCGCTGCCCGTGCGGCTGGGGCAGATTGATATGGCGAGGAAATGGATGGATGTGGGTTTGCAGCTTGCTAAGCATGTTCATGGAATGGAGACTTACAGAGCGTGCATGGAGGATTTTATCAAAGGTTTTGAGAAGAGATTTTGTGCACCAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007140 GO:0007143 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0032501 GO:0032504 GO:0034641 GO:0035822 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048232 GO:0048285 GO:0048609 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051704 GO:0061982 GO:0070192 GO:0071139 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPO22

Protein Analysis

939

Amino Acids

103.97

Weight (kDa)

7.31

Isoelectric Point (pI)

40.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPO22	PF08631	197 - 469	6.7e-34	Meiosis protein SPO22/ZIP4 like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016695)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48390
fragaria_vesca	FvH4_2g17460
malus_domestica	MD05G1153500.v1.1
prunus_persica	Prupe.8G186900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0282191
rosa_laevigata	RLG00000012903
rosa_multiflora	Rmu_sc0014549.1_g000008
rosa_roxburghii	Rroxscaffold_7G00186410
rosa_rugosa	Rorug06G0146100
rosa_samantha	Rh6AG259200 Rh6BG262500 Rh6CG261400 Rh6DG253500
rosa_wichuraiana	Rw6G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	614
AatII	GACGTC	2 cut(s)	387, 603
Acc36I	ACCTGC	1 cut(s)	1640
AccB1I	GGYRCC	1 cut(s)	1158
AccB7I	CCANNNNNTGG	2 cut(s)	697, 2485
AccBSI	CCGCTC	1 cut(s)	306
AccI	GTMKAC	1 cut(s)	279
AccII	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
AccIII	TCCGGA	3 cut(s)	142, 459, 1057
AclWI	GGATC	4 cut(s)	14, 786, 846, 1231
AcoI	YGGCCR	1 cut(s)	870
AcsI	RAATTY	4 cut(s)	1230, 1437, 1997, 2329
AcuI	CTGAAG	8 cut(s)	251, 348, 1583, 1788, 2088, 2354, 2433, 2618
AcyI	GRCGYC	4 cut(s)	384, 600, 1212, 1296
AdeI	CACNNNGTG	1 cut(s)	1201
AfaI	GTAC	3 cut(s)	785, 1007, 2567
AfiI	CCNNNNNNNGG	7 cut(s)	403, 676, 697, 1893, 1965, 2202, 2485
AflII	CTTAAG	1 cut(s)	1844
AflIII	ACRYGT	1 cut(s)	750
AhlI	ACTAGT	2 cut(s)	1379, 2320
AjiI	CACGTC	2 cut(s)	352, 1165
AjnI	CCWGG	2 cut(s)	561, 1813
AjuI	GAANNNNNNNTTGG	4 cut(s)	680, 712, 974, 1006
Alw21I	GWGCWC	5 cut(s)	18, 33, 1029, 1257, 2809
Alw44I	GTGCAC	1 cut(s)	2805
AlwI	GGATC	4 cut(s)	14, 786, 846, 1231
Ama87I	CYCGRG	1 cut(s)	1744
Aor13HI	TCCGGA	3 cut(s)	142, 459, 1057
ApaLI	GTGCAC	1 cut(s)	2805
ApeKI	GCWGC	6 cut(s)	628, 856, 1301, 1667, 2665, 2722
ApoI	RAATTY	4 cut(s)	1230, 1437, 1997, 2329
AsiSI	GCGATCGC	1 cut(s)	624
Asp700I	GAANNNNTTC	1 cut(s)	692
AspLEI	GCGC	6 cut(s)	317, 628, 682, 718, 1172, 1851
AsuC2I	CCSGG	7 cut(s)	158, 404, 1199, 1745, 1746, 1966, 2203
AsuHPI	GGTGA	6 cut(s)	418, 1213, 1220, 1229, 1304, 2231
AvaI	CYCGRG	1 cut(s)	1744
AvaII	GGWCC	7 cut(s)	565, 667, 739, 949, 1785, 1877, 1956
BaeGI	GKGCMC	1 cut(s)	2809
BanI	GGYRCC	1 cut(s)	1158
BanII	GRGCYC	4 cut(s)	18, 33, 1029, 1257
BarI	GAAGNNNNNNTAC	2 cut(s)	239, 271
BbsI	GAAGAC	3 cut(s)	1918, 2021, 2126
Bbv12I	GWGCWC	5 cut(s)	18, 33, 1029, 1257, 2809
BbvCI	CCTCAGC	1 cut(s)	311
BbvI	GCAGC	6 cut(s)	615, 843, 1288, 1679, 2652, 2734
BccI	CCATC	5 cut(s)	82, 885, 963, 2033, 2701
BceAI	ACGGC	6 cut(s)	729, 857, 1140, 1674, 2484, 2661
BcgI	CGANNNNNNTGC	4 cut(s)	610, 644, 1364, 1398
BciT130I	CCWGG	2 cut(s)	563, 1815
BciVI	GTATCC	1 cut(s)	2622
BclI	TGATCA	4 cut(s)	408, 906, 1498, 2221
BcnI	CCSGG	7 cut(s)	158, 404, 1199, 1745, 1746, 1966, 2203
BcuI	ACTAGT	2 cut(s)	1379, 2320
BfaI	CTAG	3 cut(s)	1380, 2165, 2321
BfmI	CTRYAG	3 cut(s)	498, 881, 1580
BfoI	RGCGCY	3 cut(s)	683, 719, 1173
BfrI	CTTAAG	1 cut(s)	1844
BfuAI	ACCTGC	1 cut(s)	1640
BfuI	GTATCC	1 cut(s)	2622
BglI	GCCNNNNNGGC	3 cut(s)	1178, 2645, 2674
BlpI	GCTNAGC	2 cut(s)	1350, 2730
Bme1390I	CCNGG	9 cut(s)	158, 404, 563, 1199, 1745, 1746, 1815, 1966, 2203
Bme18I	GGWCC	7 cut(s)	565, 667, 739, 949, 1785, 1877, 1956
BmeT110I	CYCGRG	1 cut(s)	1744
BmgBI	CACGTC	2 cut(s)	352, 1165
BmiI	GGNNCC	3 cut(s)	950, 1160, 1957
BmrFI	CCNGG	9 cut(s)	158, 404, 563, 1199, 1745, 1746, 1815, 1966, 2203
BmsI	GCATC	7 cut(s)	326, 582, 2021, 2388, 2527, 2539, 2669
BoxI	GACNNNNGTC	1 cut(s)	1790
BpiI	GAAGAC	3 cut(s)	1918, 2021, 2126
Bpu10I	CCTNAGC	3 cut(s)	311, 569, 1028
Bpu1102I	GCTNAGC	2 cut(s)	1350, 2730
BpuEI	CTTGAG	5 cut(s)	416, 818, 1820, 2023, 2139
BpuMI	CCSGG	7 cut(s)	158, 404, 1199, 1745, 1746, 1966, 2203
BsaBI	GATNNNNATC	2 cut(s)	521, 2378
BsaHI	GRCGYC	4 cut(s)	384, 600, 1212, 1296
BsaI	GGTCTC	1 cut(s)	1490
BsaWI	WCCGGW	4 cut(s)	142, 459, 609, 1057
Bsc4I	CCNNNNNNNGG	7 cut(s)	403, 676, 697, 1893, 1965, 2202, 2485
Bse118I	RCCGGY	3 cut(s)	437, 1214, 1759
Bse1I	ACTGG	2 cut(s)	691, 2013
Bse3DI	GCAATG	3 cut(s)	1326, 1410, 1629
Bse8I	GATNNNNATC	2 cut(s)	521, 2378
BseAI	TCCGGA	3 cut(s)	142, 459, 1057
BseBI	CCWGG	2 cut(s)	563, 1815
BseGI	GGATG	6 cut(s)	317, 955, 1831, 2542, 2712, 2716
BseJI	GATNNNNATC	2 cut(s)	521, 2378
BseLI	CCNNNNNNNGG	7 cut(s)	403, 676, 697, 1893, 1965, 2202, 2485
BseMI	GCAATG	3 cut(s)	1326, 1410, 1629
BseNI	ACTGG	2 cut(s)	691, 2013
BseRI	GAGGAG	2 cut(s)	227, 356
BseSI	GKGCMC	1 cut(s)	2809
BseX3I	CGGCCG	1 cut(s)	870
BseXI	GCAGC	6 cut(s)	615, 843, 1288, 1679, 2652, 2734
BseYI	CCCAGC	4 cut(s)	1103, 1491, 2174, 2677
BsgI	GTGCAG	1 cut(s)	1404
Bsh1236I	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
Bsh1285I	CGRYCG	3 cut(s)	610, 624, 873
BshNI	GGYRCC	1 cut(s)	1158
BsiEI	CGRYCG	3 cut(s)	610, 624, 873
BsiHKAI	GWGCWC	5 cut(s)	18, 33, 1029, 1257, 2809
BsiHKCI	CYCGRG	1 cut(s)	1744
BslFI	GGGAC	8 cut(s)	380, 611, 962, 1120, 1158, 1909, 1969, 2607
BslI	CCNNNNNNNGG	7 cut(s)	403, 676, 697, 1893, 1965, 2202, 2485
BsmBI	CGTCTC	3 cut(s)	266, 391, 607
BsmFI	GGGAC	8 cut(s)	380, 611, 962, 1120, 1158, 1909, 1969, 2607
BsmI	GAATGC	2 cut(s)	1324, 2523
Bso31I	GGTCTC	1 cut(s)	1490
BsoBI	CYCGRG	1 cut(s)	1744
Bsp1286I	GDGCHC	5 cut(s)	18, 33, 1029, 1257, 2809
Bsp13I	TCCGGA	3 cut(s)	142, 459, 1057
Bsp1407I	TGTACA	1 cut(s)	2565
Bsp1720I	GCTNAGC	2 cut(s)	1350, 2730
Bsp68I	TCGCGA	3 cut(s)	578, 620, 686
BspEI	TCCGGA	3 cut(s)	142, 459, 1057
BspFNI	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
BspLI	GGNNCC	3 cut(s)	950, 1160, 1957
BspMAI	CTGCAG	2 cut(s)	885, 1584
BspMI	ACCTGC	1 cut(s)	1640
BspPI	GGATC	4 cut(s)	14, 786, 846, 1231
BspT107I	GGYRCC	1 cut(s)	1158
BspTI	CTTAAG	1 cut(s)	1844
BspTNI	GGTCTC	1 cut(s)	1490
BsrBI	CCGCTC	1 cut(s)	306
BsrDI	GCAATG	3 cut(s)	1326, 1410, 1629
BsrFI	RCCGGY	3 cut(s)	437, 1214, 1759
BsrGI	TGTACA	1 cut(s)	2565
BsrI	ACTGG	2 cut(s)	691, 2013
BssAI	RCCGGY	3 cut(s)	437, 1214, 1759
BssNI	GRCGYC	4 cut(s)	384, 600, 1212, 1296
BssT1I	CCWWGG	3 cut(s)	1239, 1449, 1788
Bst2UI	CCWGG	2 cut(s)	563, 1815
Bst4CI	ACNGT	3 cut(s)	166, 260, 1149
Bst6I	CTCTTC	5 cut(s)	374, 813, 1289, 2464, 2789
BstACI	GRCGYC	4 cut(s)	384, 600, 1212, 1296
BstAFI	CTTAAG	1 cut(s)	1844
BstAPI	GCANNNNNTGC	2 cut(s)	1330, 2426
BstAUI	TGTACA	1 cut(s)	2565
BstDSI	CCRYGG	1 cut(s)	2466
BstF5I	GGATG	6 cut(s)	317, 955, 1831, 2542, 2712, 2716
BstFNI	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
BstH2I	RGCGCY	3 cut(s)	683, 719, 1173
BstHHI	GCGC	6 cut(s)	317, 628, 682, 718, 1172, 1851
BstMCI	CGRYCG	3 cut(s)	610, 624, 873
BstNI	CCWGG	2 cut(s)	563, 1815
BstNSI	RCATGY	2 cut(s)	2566, 2738
BstPAI	GACNNNNGTC	1 cut(s)	1790
BstSCI	CCNGG	9 cut(s)	156, 402, 561, 1197, 1743, 1744, 1813, 1964, 2201
BstSFI	CTRYAG	3 cut(s)	498, 881, 1580
BstSLI	GKGCMC	1 cut(s)	2809
BstUI	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
BstV1I	GCAGC	6 cut(s)	615, 843, 1288, 1679, 2652, 2734
BstV2I	GAAGAC	3 cut(s)	1918, 2021, 2126
BstX2I	RGATCY	2 cut(s)	778, 838
BstXI	CCANNNNNNTGG	1 cut(s)	1246
BstYI	RGATCY	2 cut(s)	778, 838
BstZI	CGGCCG	1 cut(s)	870
BsuI	GTATCC	1 cut(s)	2622
BtgI	CCRYGG	1 cut(s)	2466
BtgZI	GCGATG	5 cut(s)	60, 559, 992, 1531, 2646
BtrI	CACGTC	2 cut(s)	352, 1165
BtsCI	GGATG	6 cut(s)	317, 955, 1831, 2542, 2712, 2716
BtsIMutI	CAGTG	2 cut(s)	227, 1335
BtuMI	TCGCGA	3 cut(s)	578, 620, 686
BveI	ACCTGC	1 cut(s)	1640
CfoI	GCGC	6 cut(s)	317, 628, 682, 718, 1172, 1851
Cfr10I	RCCGGY	3 cut(s)	437, 1214, 1759
Cfr9I	CCCGGG	1 cut(s)	1744
CseI	GACGC	7 cut(s)	265, 758, 862, 1220, 1304, 1840, 1854
Csp6I	GTAC	3 cut(s)	784, 1006, 2566
CviQI	GTAC	3 cut(s)	784, 1006, 2566
DraIII	CACNNNGTG	1 cut(s)	1201
DrdI	GACNNNNNNGTC	1 cut(s)	614
DseDI	GACNNNNNNGTC	1 cut(s)	614
EaeI	YGGCCR	1 cut(s)	870
EagI	CGGCCG	1 cut(s)	870
Eam1104I	CTCTTC	5 cut(s)	374, 813, 1289, 2464, 2789
EarI	CTCTTC	5 cut(s)	374, 813, 1289, 2464, 2789
EciI	GGCGGA	5 cut(s)	160, 170, 335, 406, 1265
Ecl136II	GAGCTC	4 cut(s)	16, 31, 1027, 1255
EclXI	CGGCCG	1 cut(s)	870
Eco130I	CCWWGG	3 cut(s)	1239, 1449, 1788
Eco24I	GRGCYC	4 cut(s)	18, 33, 1029, 1257
Eco31I	GGTCTC	1 cut(s)	1490
Eco47I	GGWCC	7 cut(s)	565, 667, 739, 949, 1785, 1877, 1956
Eco52I	CGGCCG	1 cut(s)	870
Eco53kI	GAGCTC	4 cut(s)	16, 31, 1027, 1255
Eco57I	CTGAAG	8 cut(s)	251, 348, 1583, 1788, 2088, 2354, 2433, 2618
Eco88I	CYCGRG	1 cut(s)	1744
EcoICRI	GAGCTC	4 cut(s)	16, 31, 1027, 1255
EcoO109I	RGGNCCY	1 cut(s)	739
EcoRI	GAATTC	3 cut(s)	1230, 1437, 1997
EcoRII	CCWGG	2 cut(s)	561, 1813
EcoT14I	CCWWGG	3 cut(s)	1239, 1449, 1788
EcoT22I	ATGCAT	1 cut(s)	1326
EcoT38I	GRGCYC	4 cut(s)	18, 33, 1029, 1257
ErhI	CCWWGG	3 cut(s)	1239, 1449, 1788
Esp3I	CGTCTC	3 cut(s)	266, 391, 607
FalI	AAGNNNNNCTT	2 cut(s)	1392, 1424
FaqI	GGGAC	8 cut(s)	380, 611, 962, 1120, 1158, 1909, 1969, 2607
FauNDI	CATATG	1 cut(s)	446
FbaI	TGATCA	4 cut(s)	408, 906, 1498, 2221
FblI	GTMKAC	1 cut(s)	279
FokI	GGATG	6 cut(s)	304, 942, 1838, 2549, 2719, 2723
FriOI	GRGCYC	4 cut(s)	18, 33, 1029, 1257
FspBI	CTAG	3 cut(s)	1380, 2165, 2321
GlaI	GCGC	6 cut(s)	316, 627, 681, 717, 1171, 1850
GsaI	CCCAGC	4 cut(s)	1107, 1495, 2178, 2681
HaeII	RGCGCY	3 cut(s)	683, 719, 1173
HgaI	GACGC	7 cut(s)	265, 758, 862, 1220, 1304, 1840, 1854
HhaI	GCGC	6 cut(s)	317, 628, 682, 718, 1172, 1851
Hin1I	GRCGYC	4 cut(s)	384, 600, 1212, 1296
Hin6I	GCGC	6 cut(s)	315, 626, 680, 716, 1170, 1849
HinP1I	GCGC	6 cut(s)	315, 626, 680, 716, 1170, 1849
HincII	GTYRAC	1 cut(s)	280
HindII	GTYRAC	1 cut(s)	280
HindIII	AAGCTT	1 cut(s)	554
HphI	GGTGA	6 cut(s)	418, 1213, 1220, 1229, 1304, 2231
Hpy166II	GTNNAC	2 cut(s)	280, 2807
Hpy8I	GTNNAC	2 cut(s)	280, 2807
Hpy99I	CGWCG	6 cut(s)	281, 356, 386, 752, 2549, 2620
HpyCH4III	ACNGT	3 cut(s)	166, 260, 1149
HpyCH4IV	ACGT	4 cut(s)	351, 384, 600, 1164
HpySE526I	ACGT	4 cut(s)	351, 384, 600, 1164
Hsp92I	GRCGYC	4 cut(s)	384, 600, 1212, 1296
HspAI	GCGC	6 cut(s)	315, 626, 680, 716, 1170, 1849
Kpn2I	TCCGGA	3 cut(s)	142, 459, 1057
KroI	GCCGGC	1 cut(s)	1759
KroNI	GCCGGC	1 cut(s)	1761
Ksp22I	TGATCA	4 cut(s)	408, 906, 1498, 2221
Lsp1109I	GCAGC	6 cut(s)	615, 843, 1288, 1679, 2652, 2734
LweI	GCATC	7 cut(s)	326, 582, 2021, 2388, 2527, 2539, 2669
MaeI	CTAG	3 cut(s)	1380, 2165, 2321
MaeII	ACGT	4 cut(s)	351, 384, 600, 1164
MaeIII	GTNAC	6 cut(s)	254, 1208, 1292, 1792, 1936, 2515
MbiI	CCGCTC	1 cut(s)	306
MflI	RGATCY	2 cut(s)	778, 838
MhlI	GDGCHC	5 cut(s)	18, 33, 1029, 1257, 2809
MluI	ACGCGT	1 cut(s)	750
MlyI	GAGTC	2 cut(s)	119, 2294
MmeI	TCCRAC	3 cut(s)	630, 1888, 2639
Mph1103I	ATGCAT	1 cut(s)	1326
MroI	TCCGGA	3 cut(s)	142, 459, 1057
MroNI	GCCGGC	1 cut(s)	1759
MroXI	GAANNNNTTC	1 cut(s)	692
MseI	TTAA	3 cut(s)	1845, 2075, 2815
MslI	CAYNNNNRTG	3 cut(s)	1860, 2513, 2739
MspA1I	CMGCKG	2 cut(s)	359, 1301
MspCI	CTTAAG	1 cut(s)	1844
MspR9I	CCNGG	9 cut(s)	158, 404, 563, 1199, 1745, 1746, 1815, 1966, 2203
Mva1269I	GAATGC	2 cut(s)	1324, 2523
MvaI	CCWGG	2 cut(s)	563, 1815
MvnI	CGCG	8 cut(s)	293, 578, 620, 626, 686, 752, 816, 1851
NaeI	GCCGGC	1 cut(s)	1761
NciI	CCSGG	7 cut(s)	158, 404, 1199, 1745, 1746, 1966, 2203
NdeI	CATATG	1 cut(s)	446
NgoMIV	GCCGGC	1 cut(s)	1759
NlaIV	GGNNCC	3 cut(s)	950, 1160, 1957
NmeAIII	GCCGAG	4 cut(s)	37, 1036, 1042, 1157
NmuCI	GTSAC	5 cut(s)	1208, 1292, 1792, 1936, 2515
NruI	TCGCGA	3 cut(s)	578, 620, 686
NsiI	ATGCAT	1 cut(s)	1326
NspI	RCATGY	2 cut(s)	2566, 2738
PcsI	WCGNNNNNNNCGW	4 cut(s)	276, 974, 1014, 1263
PctI	GAATGC	2 cut(s)	1324, 2523
PdiI	GCCGGC	1 cut(s)	1761
PdmI	GAANNNNTTC	1 cut(s)	692
PflFI	GACNNNGTC	2 cut(s)	164, 530
PflMI	CCANNNNNTGG	2 cut(s)	697, 2485
PfoI	TCCNGGA	1 cut(s)	561
Ple19I	CGATCG	1 cut(s)	624
PleI	GAGTC	2 cut(s)	118, 2294
PpsI	GAGTC	2 cut(s)	118, 2294
PpuMI	RGGWCCY	1 cut(s)	739
PshAI	GACNNNNGTC	1 cut(s)	1790
Psp124BI	GAGCTC	4 cut(s)	18, 33, 1029, 1257
Psp5II	RGGWCCY	1 cut(s)	739
Psp6I	CCWGG	2 cut(s)	561, 1813
PspFI	CCCAGC	4 cut(s)	1103, 1491, 2174, 2677
PspGI	CCWGG	2 cut(s)	561, 1813
PspN4I	GGNNCC	3 cut(s)	950, 1160, 1957
PspPPI	RGGWCCY	1 cut(s)	739
PstI	CTGCAG	2 cut(s)	885, 1584
PsuI	RGATCY	2 cut(s)	778, 838
PsyI	GACNNNGTC	2 cut(s)	164, 530
PvuI	CGATCG	1 cut(s)	624
RgaI	GCGATCGC	1 cut(s)	624
RruI	TCGCGA	3 cut(s)	578, 620, 686
RsaI	GTAC	3 cut(s)	785, 1007, 2567
RsaNI	GTAC	3 cut(s)	784, 1006, 2566
RseI	CAYNNNNRTG	3 cut(s)	1860, 2513, 2739
SacI	GAGCTC	4 cut(s)	18, 33, 1029, 1257
SalI	GTCGAC	1 cut(s)	278
SaqAI	TTAA	3 cut(s)	1845, 2075, 2815
SchI	GAGTC	2 cut(s)	119, 2294
ScrFI	CCNGG	9 cut(s)	158, 404, 563, 1199, 1745, 1746, 1815, 1966, 2203
SduI	GDGCHC	5 cut(s)	18, 33, 1029, 1257, 2809
SfaAI	GCGATCGC	1 cut(s)	624
SfaNI	GCATC	7 cut(s)	326, 582, 2021, 2388, 2527, 2539, 2669
SfcI	CTRYAG	3 cut(s)	498, 881, 1580
SgfI	GCGATCGC	1 cut(s)	624
SgrAI	CRCCGGYG	1 cut(s)	1214
SinI	GGWCC	7 cut(s)	565, 667, 739, 949, 1785, 1877, 1956
SmaI	CCCGGG	1 cut(s)	1746
SmiMI	CAYNNNNRTG	3 cut(s)	1860, 2513, 2739
SmlI	CTYRAG	6 cut(s)	431, 833, 1835, 1844, 2002, 2154
SmoI	CTYRAG	6 cut(s)	431, 833, 1835, 1844, 2002, 2154
SpeI	ACTAGT	2 cut(s)	1379, 2320
SspMI	CTAG	3 cut(s)	1380, 2165, 2321
SstI	GAGCTC	4 cut(s)	18, 33, 1029, 1257
StyD4I	CCNGG	9 cut(s)	156, 402, 561, 1197, 1743, 1744, 1813, 1964, 2201
StyI	CCWWGG	3 cut(s)	1239, 1449, 1788
TaaI	ACNGT	3 cut(s)	166, 260, 1149
TaiI	ACGT	4 cut(s)	354, 387, 603, 1167
TatI	WGTACW	1 cut(s)	2565
TauI	GCSGC	6 cut(s)	296, 359, 1103, 1301, 1995, 2677
Tru1I	TTAA	3 cut(s)	1845, 2075, 2815
Tru9I	TTAA	3 cut(s)	1845, 2075, 2815
TscAI	CASTG	2 cut(s)	234, 1335
TseFI	GTSAC	5 cut(s)	1208, 1292, 1792, 1936, 2515
TseI	GCWGC	6 cut(s)	628, 856, 1301, 1667, 2665, 2722
Tsp45I	GTSAC	5 cut(s)	1208, 1292, 1792, 1936, 2515
TspGWI	ACGGA	3 cut(s)	1798, 2619, 2633
TspMI	CCCGGG	1 cut(s)	1744
TspRI	CASTG	2 cut(s)	234, 1335
Tth111I	GACNNNGTC	2 cut(s)	164, 530
Van91I	CCANNNNNTGG	2 cut(s)	697, 2485
Vha464I	CTTAAG	1 cut(s)	1844
VneI	GTGCAC	1 cut(s)	2805
VpaK11BI	GGWCC	7 cut(s)	565, 667, 739, 949, 1785, 1877, 1956
XapI	RAATTY	4 cut(s)	1230, 1437, 1997, 2329
XbaI	TCTAGA	1 cut(s)	2164
XceI	RCATGY	2 cut(s)	2566, 2738
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1202
XmaI	CCCGGG	1 cut(s)	1744
XmiI	GTMKAC	1 cut(s)	279
XmnI	GAANNNNTTC	1 cut(s)	692
XspI	CTAG	3 cut(s)	1380, 2165, 2321
ZraI	GACGTC	2 cut(s)	385, 601
Zsp2I	ATGCAT	1 cut(s)	1326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.