FvH4_2g23150

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012898
Physical Location & Seq
Forward (+)
18979932 .. 18981714
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23150.t1

Sequence Viewer

Length: 591 bp
ATGACAAAAGCAAGACGGCTTGGGGTGTATACTCCGGTGCTCTACGCTGTGGACACTGTGCTGCATACCCTCACATTTGAATATGTGGAGGGTCCTTCTGTTAAAGATGTGTTTCTTGAGTTTGGGTTAGGCGGTGGTGGTGTGGCTGCCGAACGTATGGATGATATTGCAATGCAGATTGGTCAGGCTATTGGAAAGCTGCATGATGGTGGCCTAATTCATGGGGACTTGACCACATCTAACCTGCTAATTAGAGCTGCTACCGATCAAGTGGTTGTTATTGATTTTGGTCTGAGCTTTACATCAACCCTTCCAGAAGATAAAGCTGTGGATTTGTATGTACTGGAACGAGCTTTGCTCTCGATGCATTCTTCGTGTGGGAATGTGATGGATCGCATACTTGCTGCATATCGCAAATCCTCAAAGCAGTGGTCATCCACTTTTAACAAATTAGCCCAAGGTGGGTCCTTAATCGTCTGCTCACTCTTTCATTTTGTGTTATCCTTTACTTTTGGCTTTCTTTCTTGTTCTCACTGCTTCACAACTTTTACAGTGAGACAACGAGGCCGAAAGCGCACCATGATCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

197

Amino Acids

21.47

Weight (kDa)

8.37

Isoelectric Point (pI)

30.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 9 - 99 3.4e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 23 - 101 4e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 25 - 101 4.6e-06 Protein tyrosine and serine/threonine kinase
Kdo PF06293 53 - 122 6.9e-06 Lipopolysaccharide kinase (Kdo/WaaP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26110 AT5G26110 AT5G26110
fragaria_vesca FvH4_2g23150
malus_domestica MD11G1280900.v1.1
prunus_persica Prupe.8G236800_v2.0.a1
pyrus_communis pycom11g24870
rosa_chinensis RchiOBHm_Chr6g0290401
rosa_laevigata RLG00000012222
rosa_multiflora Rmu_sc0029478.1_g000001
rosa_rugosa Rorug06G0212500 Rorug06G0212600
rosa_samantha Rh6AG322500 Rh6BG329900 Rh6CG335900 Rh6DG322800
rosa_wichuraiana Rw6G027860 Rw6G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 29
AciI CCGC 1 cut(s) 132
AclWI GGATC 1 cut(s) 399
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 1 cut(s) 462
AgsI TTSAA 1 cut(s) 80
AluBI AGCT 5 cut(s) 199, 257, 297, 326, 353
AluI AGCT 5 cut(s) 199, 257, 297, 326, 353
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 550
AlwI GGATC 1 cut(s) 399
AoxI GGCC 2 cut(s) 211, 565
ApeKI GCWGC 5 cut(s) 61, 146, 199, 257, 404
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 2 cut(s) 92, 465
AvaII GGWCC 2 cut(s) 92, 465
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 5 cut(s) 48, 133, 186, 244, 391
BccI CCATC 2 cut(s) 200, 382
BceAI ACGGC 1 cut(s) 32
BcgI CGANNNNNNTGC 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 5 cut(s) 62, 147, 200, 258, 405
BlsI GCNGC 5 cut(s) 63, 148, 201, 259, 406
Bme18I GGWCC 2 cut(s) 92, 465
BmgT120I GGNCC 2 cut(s) 92, 465
BmiI GGNNCC 2 cut(s) 93, 466
BmsI GCATC 1 cut(s) 354
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpuEI CTTGAG 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 2 cut(s) 166, 434
BseLI CCNNNNNNNGG 1 cut(s) 462
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 5 cut(s) 48, 133, 186, 244, 391
BshFI GGCC 2 cut(s) 213, 567
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 462
BsmAI GTCTC 1 cut(s) 550
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 213, 567
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 3 cut(s) 265, 391, 582
BspACI CCGC 1 cut(s) 132
BspANI GGCC 2 cut(s) 213, 567
BspCNI CTCAG 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 93, 466
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 3 cut(s) 265, 391, 582
BssNAI GTATAC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 457
Bst1107I GTATAC 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 58, 553
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 166, 434
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 3 cut(s) 268, 394, 585
BstMAI GTCTC 1 cut(s) 550
BstMBI GATC 3 cut(s) 265, 391, 582
BstMWI GCNNNNNNNGC 2 cut(s) 364, 573
BstV1I GCAGC 5 cut(s) 48, 133, 186, 244, 391
BstZ17I GTATAC 1 cut(s) 30
BsuRI GGCC 2 cut(s) 213, 567
BtsCI GGATG 2 cut(s) 166, 434
BtsI GCAGTG 2 cut(s) 434, 532
BtsIMutI CAGTG 4 cut(s) 54, 434, 532, 558
BveI ACCTGC 1 cut(s) 252
CfoI GCGC 1 cut(s) 576
Cfr13I GGNCC 2 cut(s) 92, 465
Csp6I GTAC 1 cut(s) 341
CviAII CATG 3 cut(s) 203, 221, 580
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 1 cut(s) 293
DpnI GATC 3 cut(s) 267, 393, 584
DpnII GATC 3 cut(s) 265, 391, 582
Eco130I CCWWGG 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 92, 465
EcoO109I RGGNCCY 2 cut(s) 92, 465
EcoT14I CCWWGG 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 3 cut(s) 206, 224, 583
FaqI GGGAC 1 cut(s) 239
FatI CATG 3 cut(s) 202, 220, 579
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 5 cut(s) 62, 147, 200, 258, 405
FokI GGATG 2 cut(s) 173, 421
Fsp4HI GCNGC 5 cut(s) 62, 147, 200, 258, 405
GlaI GCGC 1 cut(s) 575
GluI GCNGC 5 cut(s) 62, 147, 200, 258, 405
HaeIII GGCC 2 cut(s) 213, 567
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 576
Hin1II CATG 3 cut(s) 206, 224, 583
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 2 cut(s) 30, 52
Hpy188I TCNGA 2 cut(s) 294, 587
Hpy188III TCNNGA 3 cut(s) 116, 314, 361
Hpy8I GTNNAC 2 cut(s) 30, 52
HpyAV CCTTC 2 cut(s) 105, 320
HpyCH4III ACNGT 2 cut(s) 58, 553
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 6 cut(s) 64, 170, 175, 202, 367, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 573
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 3 cut(s) 206, 224, 583
HspAI GCGC 1 cut(s) 574
Kzo9I GATC 3 cut(s) 265, 391, 582
LpnPI CCDG 5 cut(s) 48, 170, 257, 327, 329
Lsp1109I GCAGC 5 cut(s) 48, 133, 186, 244, 391
LweI GCATC 1 cut(s) 354
MaeII ACGT 1 cut(s) 154
MalI GATC 3 cut(s) 267, 393, 584
MboI GATC 3 cut(s) 265, 391, 582
MboII GAAGA 2 cut(s) 329, 363
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 3 cut(s) 216, 249, 449
MnlI CCTC 4 cut(s) 80, 82, 430, 557
Mph1103I ATGCAT 1 cut(s) 369
MseI TTAA 3 cut(s) 102, 444, 470
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 367
MwoI GCNNNNNNNGC 2 cut(s) 364, 573
NdeII GATC 3 cut(s) 265, 391, 582
NlaIII CATG 3 cut(s) 206, 224, 583
NlaIV GGNNCC 2 cut(s) 93, 466
NsiI ATGCAT 1 cut(s) 369
PctI GAATGC 1 cut(s) 367
PkrI GCNGC 5 cut(s) 63, 148, 201, 259, 406
PpuMI RGGWCCY 2 cut(s) 92, 465
Psp5II RGGWCCY 2 cut(s) 92, 465
PspN4I GGNNCC 2 cut(s) 93, 466
PspPI GGNCC 2 cut(s) 92, 465
PspPPI RGGWCCY 2 cut(s) 92, 465
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 3 cut(s) 102, 444, 470
SatI GCNGC 5 cut(s) 62, 147, 200, 258, 405
Sau3AI GATC 3 cut(s) 265, 391, 582
Sau96I GGNCC 2 cut(s) 92, 465
SduI GDGCHC 1 cut(s) 42
SetI ASST 8 cut(s) 157, 201, 246, 259, 299, 328, 355, 463
SfaNI GCATC 1 cut(s) 354
SinI GGWCC 2 cut(s) 92, 465
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 3 cut(s) 216, 249, 449
SsiI CCGC 1 cut(s) 132
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 2 cut(s) 58, 553
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 362
TasI AATT 3 cut(s) 216, 249, 449
TatI WGTACW 1 cut(s) 340
Tru1I TTAA 3 cut(s) 102, 444, 470
Tru9I TTAA 3 cut(s) 102, 444, 470
TscAI CASTG 4 cut(s) 61, 434, 539, 558
TseI GCWGC 5 cut(s) 61, 146, 199, 257, 404
TspDTI ATGAA 2 cut(s) 209, 479
TspRI CASTG 4 cut(s) 61, 434, 539, 558
VpaK11BI GGWCC 2 cut(s) 92, 465
XmiI GTMKAC 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.