Rw6G027960

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012898
Physical Location & Seq
Forward (+)
51914979 .. 51916308
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027960.1

Sequence Viewer

Length: 687 bp
ATGGAAGTTGGGGCAGATTCTGTAGATAGCTCCCTTGTTCTGGTGAAGCAAGGAGCTGAAGCTAGGGTTTTTGAGTCAACCTTTGTGGGAAGGAGGTCTATTGTGAAGGAACGGTTTTCGAAGAAGTACAGGCATCCTACTTTGGATGCAAAACTTACACTCAGGCGCCTCAATGCGGAGGCGAGGTGCATGACGAAAGCAAGACGGCTTGGGGTGCATACTCCGGTGCTTTACGCTGTGGACACTGTGCTGCATACCCTGACATTTGAATATGTGGAGGGGCCTTCTGTAAAAGATGTGTTTCTTGAGTTTGGGTTAGGAGGTGGTCGTGTGGCTGCGGAACGTATGGATGATATTGCAATGCAGATTGGTCAGGCTATTGGAAAGCTGCATGATGGTGGCCTGATTCATGGGGACTTGACCACATCTAACCTGCTAATTAGAGCTGCTACTAATCAAGTGGTTGTTATTGATTTCGGTTTGAGCTCTACATCAACCCTTCCAGAAGATAAAGCTGTGGATTTGTATGTACTGGAACGAGCTTTGCTCTCAATGCATTCTTCATGTGGGAATGTGATGGATCGCATACTTGCTGCATATCGCAAATCCTCAAAGCAGTGGTCATCCACTTTTAACAAATTAGCTCAAGTGAGACAACGAGGCCGAAAGCGCACCATGATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

228

Amino Acids

25.2

Weight (kDa)

9.72

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIO1 PF01163 26 - 162 5.3e-06 RIO domain
PK_Tyr_Ser-Thr PF07714 54 - 165 2.7e-08 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 55 - 166 2.5e-10 Protein kinase domain
ABC1 PF03109 71 - 164 1.8e-07 ABC1 atypical kinase-like domain
Kdo PF06293 115 - 186 2e-06 Lipopolysaccharide kinase (Kdo/WaaP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26110 AT5G26110 AT5G26110
fragaria_vesca FvH4_2g23150
malus_domestica MD11G1280900.v1.1
prunus_persica Prupe.8G236800_v2.0.a1
pyrus_communis pycom11g24870
rosa_chinensis RchiOBHm_Chr6g0290401
rosa_laevigata RLG00000012222
rosa_multiflora Rmu_sc0029478.1_g000001
rosa_rugosa Rorug06G0212500 Rorug06G0212600
rosa_samantha Rh6AG322500 Rh6BG329900 Rh6CG335900 Rh6DG322800
rosa_wichuraiana Rw6G027860 Rw6G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 165
AciI CCGC 2 cut(s) 176, 338
AclWI GGATC 1 cut(s) 588
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 2 cut(s) 128, 531
AfiI CCNNNNNNNGG 2 cut(s) 40, 175
AgsI TTSAA 1 cut(s) 269
AluBI AGCT 9 cut(s) 30, 56, 62, 388, 446, 486, 515, 542, 644
AluI AGCT 9 cut(s) 30, 56, 62, 388, 446, 486, 515, 542, 644
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 1 cut(s) 646
AlwI GGATC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 3 cut(s) 281, 400, 661
ApeKI GCWGC 5 cut(s) 250, 335, 388, 446, 593
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
AspLEI GCGC 2 cut(s) 168, 672
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 119
BanI GGYRCC 1 cut(s) 165
BanII GRGCYC 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 5 cut(s) 237, 322, 375, 433, 580
BccI CCATC 2 cut(s) 389, 571
BceAI ACGGC 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 646
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 441
BisI GCNGC 5 cut(s) 251, 336, 389, 447, 594
BlsI GCNGC 5 cut(s) 252, 337, 390, 448, 595
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 167, 282
BmsI GCATC 2 cut(s) 136, 142
BplI GAGNNNNNCTC 2 cut(s) 531, 563
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 326, 630
BsaHI GRCGYC 1 cut(s) 166
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 175
Bse1I ACTGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 366
BseGI GGATG 4 cut(s) 133, 151, 355, 623
BseLI CCNNNNNNNGG 2 cut(s) 40, 175
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 537
BseXI GCAGC 5 cut(s) 237, 322, 375, 433, 580
BshFI GGCC 3 cut(s) 283, 402, 663
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 488
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 40, 175
BsmAI GTCTC 1 cut(s) 646
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 283, 402, 663
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 580
BspACI CCGC 2 cut(s) 176, 338
BspANI GGCC 3 cut(s) 283, 402, 663
BspCNI CTCAG 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 167, 282
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 1 cut(s) 588
BspT104I TTCGAA 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 537
BssMI GATC 1 cut(s) 580
BssNI GRCGYC 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 114, 247
BstACI GRCGYC 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 161
BstF5I GGATG 4 cut(s) 133, 151, 355, 623
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 2 cut(s) 168, 672
BstKTI GATC 1 cut(s) 583
BstMAI GTCTC 1 cut(s) 646
BstMBI GATC 1 cut(s) 580
BstMWI GCNNNNNNNGC 3 cut(s) 214, 553, 669
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 5 cut(s) 237, 322, 375, 433, 580
BsuRI GGCC 3 cut(s) 283, 402, 663
BtsCI GGATG 4 cut(s) 133, 151, 355, 623
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 2 cut(s) 243, 623
BveI ACCTGC 1 cut(s) 441
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 2 cut(s) 168, 672
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 127, 530
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 5 cut(s) 190, 392, 410, 564, 676
CviQI GTAC 2 cut(s) 127, 530
DdeI CTNAG 1 cut(s) 161
DinI GGCGCC 1 cut(s) 167
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
Ecl136II GAGCTC 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 488
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 558
EcoT38I GRGCYC 1 cut(s) 488
EgeI GGCGCC 1 cut(s) 167
EheI GGCGCC 1 cut(s) 167
FaeI CATG 5 cut(s) 193, 395, 413, 567, 679
FaqI GGGAC 1 cut(s) 428
FatI CATG 5 cut(s) 189, 391, 409, 563, 675
Fnu4HI GCNGC 5 cut(s) 251, 336, 389, 447, 594
FokI GGATG 4 cut(s) 120, 158, 362, 610
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 5 cut(s) 251, 336, 389, 447, 594
FspBI CTAG 1 cut(s) 63
GlaI GCGC 2 cut(s) 167, 671
GluI GCNGC 5 cut(s) 251, 336, 389, 447, 594
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 3 cut(s) 283, 402, 663
HapII CCGG 1 cut(s) 224
HhaI GCGC 2 cut(s) 168, 672
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 5 cut(s) 193, 395, 413, 567, 679
Hin6I GCGC 2 cut(s) 166, 670
HinP1I GCGC 2 cut(s) 166, 670
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 3 cut(s) 17, 74, 406
HpaII CCGG 1 cut(s) 224
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 78, 241
Hpy188III TCNNGA 2 cut(s) 305, 503
Hpy8I GTNNAC 2 cut(s) 78, 241
HpyAV CCTTC 4 cut(s) 84, 100, 294, 509
HpyCH4III ACNGT 2 cut(s) 114, 247
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 9 cut(s) 149, 189, 217, 253, 359, 364, 391, 556, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 553, 669
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 5 cut(s) 193, 395, 413, 567, 679
HspAI GCGC 2 cut(s) 166, 670
KasI GGCGCC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 580
LmnI GCTCC 2 cut(s) 35, 53
Lsp1109I GCAGC 5 cut(s) 237, 322, 375, 433, 580
LweI GCATC 2 cut(s) 136, 142
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 343
MalI GATC 1 cut(s) 582
MboI GATC 1 cut(s) 580
MboII GAAGA 3 cut(s) 133, 518, 552
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 2 cut(s) 438, 638
Mly113I GGCGCC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 8 cut(s) 87, 172, 177, 179, 271, 314, 619, 653
Mph1103I ATGCAT 1 cut(s) 558
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 556
MwoI GCNNNNNNNGC 3 cut(s) 214, 553, 669
NarI GGCGCC 1 cut(s) 166
NdeII GATC 1 cut(s) 580
NlaIII CATG 5 cut(s) 193, 395, 413, 567, 679
NlaIV GGNNCC 2 cut(s) 167, 282
NsiI ATGCAT 1 cut(s) 558
NspV TTCGAA 1 cut(s) 119
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 2 cut(s) 17, 406
PkrI GCNGC 5 cut(s) 252, 337, 390, 448, 595
PleI GAGTC 1 cut(s) 82
PluTI GGCGCC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 488
PspN4I GGNNCC 2 cut(s) 167, 282
PspPI GGNCC 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 128, 531
RsaNI GTAC 2 cut(s) 127, 530
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 488
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 5 cut(s) 251, 336, 389, 447, 594
Sau3AI GATC 1 cut(s) 580
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 2 cut(s) 136, 142
SfcI CTRYAG 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 2 cut(s) 305, 645
SmoI CTYRAG 2 cut(s) 305, 645
Sse9I AATT 2 cut(s) 438, 638
SsiI CCGC 2 cut(s) 176, 338
SspDI GGCGCC 1 cut(s) 165
SspMI CTAG 1 cut(s) 63
SstI GAGCTC 1 cut(s) 488
TaaI ACNGT 2 cut(s) 114, 247
TaiI ACGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 119
TasI AATT 2 cut(s) 438, 638
TatI WGTACW 2 cut(s) 126, 529
TfiI GAWTC 2 cut(s) 17, 406
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 2 cut(s) 250, 623
TseI GCWGC 5 cut(s) 250, 335, 388, 446, 593
TspDTI ATGAA 2 cut(s) 398, 552
TspRI CASTG 2 cut(s) 250, 623
XspI CTAG 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.