Rorug06G0212500

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012898
Physical Location & Seq
Forward (+)
33826068 .. 33832623
6556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0212500.1

Sequence Viewer

Length: 1155 bp
ATGGAACAATTAAAGGATAGAAATCTAGAGTGTTGCAAAAACCTTAAAACTCTTCCGATCATCATCTATCAGTTGAAACATCTGCGGCAACTTGAGGTCAGAGGCTGTCCAAAACTTCTTACATTTCCAATGTCAACGACAACATCCACCATCATGCAGTATTTATCCGATGACAATAATGATGAAGACTGCTCAGTACCGGTGTTTCCTGAGCTGATGTTTTTGCGAGTCGGAGACTGCAACATATCAGAATGTGATTTCTTGATTCCATTTAGTTGCTTGTCCACCCTCACCTTTCTTGATCTATCAGGAAGCAGTTTTGGTAGCCTTCCAACATGGATTATCAAGTTCGGTAACTTGCAGTGGCTCATATTACGCGATTGCAAGAGGCTTCAAGAAATTCCACAGCTTTCTCCAAGCATAAAAGGAATAAATGCAAGTGGCTGCAAATCATTGGAAAGATTTTCAAAATTTTCCGATATTTTGGACCACCAAAACTCATTGGGTTGGCTTCAGTGGAGCGACTTATCTGAATGCCACAAACTACTCCATACTATGGACTTGGATGTGGAAAAGATGGCATCTATCTTACTTAACCAACACCATGATCAGGGGAAGGATTCATACTTCGAGTTCGGTGTTGTACTTCCAGGAAATAATATTCCAAAGTGGTTCAACTTTTGTAAGCATCCAACTGATCATGAGTATTGCGAGTTTGTTATTAAACTTCCTCCAAATTTCAGTGGAAAGAATTCGAGATTGGCTAAAGAAAACGATGTGGTGAAGAAAAACTATTCTTTATTGATTTTTTCTTCAATACAGTTGCCTACAACTTATACACACTTTGCTGATACTCGATCCACAATTCTAGGGAATCAGTATGGTAATACAACTCAGACTATCAATCTAGGATACCAGAATGACTACAATCCTAATCTAGGATATTGCAATTATTTCATTCCTAATTACACGGGATTCTTTCCCTCAATACTCCCCCTCAAGTTGGAGTGTATGTTGATCACTCTCAACTTGCTCAGCAGCATAGTAAACTGGTTTGAGCTCAACGGTTTGGTAAACAAGTCAGCTGGTTGCTCCTTGGTTCGGATGTGCATTGTTTTGATTAGTCCTCTTTGCACCTTCTCACGCACAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

384

Amino Acids

43.83

Weight (kDa)

6.31

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26110 AT5G26110 AT5G26110
fragaria_vesca FvH4_2g23150
malus_domestica MD11G1280900.v1.1
prunus_persica Prupe.8G236800_v2.0.a1
pyrus_communis pycom11g24870
rosa_chinensis RchiOBHm_Chr6g0290401
rosa_laevigata RLG00000012222
rosa_multiflora Rmu_sc0029478.1_g000001
rosa_rugosa Rorug06G0212500 Rorug06G0212600
rosa_samantha Rh6AG322500 Rh6BG329900 Rh6CG335900 Rh6DG322800
rosa_wichuraiana Rw6G027860 Rw6G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 556
AccII CGCG 1 cut(s) 378
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 852
AcsI RAATTY 4 cut(s) 399, 470, 736, 751
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 2 cut(s) 198, 645
AfiI CCNNNNNNNGG 5 cut(s) 556, 610, 938, 1003, 1101
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 5 cut(s) 76, 395, 468, 676, 816
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 743, 775
AluBI AGCT 4 cut(s) 214, 409, 1060, 1085
AluI AGCT 4 cut(s) 214, 409, 1060, 1085
Alw21I GWGCWC 1 cut(s) 1062
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 852
AlwNI CAGNNNCTG 1 cut(s) 105
ApeKI GCWGC 2 cut(s) 444, 1038
ApoI RAATTY 4 cut(s) 399, 470, 736, 751
AsiGI ACCGGT 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 283, 793
AvaII GGWCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 1062
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 1062
BbvI GCAGC 2 cut(s) 431, 1050
BccI CCATC 2 cut(s) 158, 571
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 905
BclI TGATCA 3 cut(s) 607, 697, 1017
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 4 cut(s) 26, 869, 908, 938
BfuI GTATCC 1 cut(s) 905
BisI GCNGC 3 cut(s) 86, 445, 1039
BlpI GCTNAGC 1 cut(s) 1034
BlsI GCNGC 3 cut(s) 87, 446, 1040
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 651
BmsI GCATC 2 cut(s) 590, 697
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 210
Bpu1102I GCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 2 cut(s) 113, 983
BsaBI GATNNNNATC 2 cut(s) 21, 62
BsaJI CCNNGG 1 cut(s) 1095
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 5 cut(s) 556, 610, 938, 1003, 1101
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 1055
Bse8I GATNNNNATC 2 cut(s) 21, 62
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 1095
BseGI GGATG 4 cut(s) 143, 571, 688, 1110
BseJI GATNNNNATC 2 cut(s) 21, 62
BseLI CCNNNNNNNGG 5 cut(s) 556, 610, 938, 1003, 1101
BseMII CTCAG 4 cut(s) 201, 207, 908, 1048
BseNI ACTGG 1 cut(s) 1055
BseXI GCAGC 2 cut(s) 431, 1050
Bsh1236I CGCG 1 cut(s) 378
BshTI ACCGGT 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 1062
BsiSI CCGG 1 cut(s) 200
BslI CCNNNNNNNGG 5 cut(s) 556, 610, 938, 1003, 1101
BsmAI GTCTC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 1062
Bsp143I GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
Bsp1720I GCTNAGC 1 cut(s) 1034
BspACI CCGC 1 cut(s) 85
BspCNI CTCAG 4 cut(s) 202, 206, 907, 1047
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 700
BspPI GGATC 1 cut(s) 852
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 1 cut(s) 1055
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 1095
BssMI GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
BssT1I CCWWGG 1 cut(s) 1095
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 2 cut(s) 822, 1067
Bst6I CTCTTC 1 cut(s) 57
BstDEI CTNAG 4 cut(s) 193, 210, 894, 1034
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 4 cut(s) 143, 571, 688, 1110
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 6 cut(s) 60, 304, 610, 700, 860, 1020
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 649
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 431, 1050
BstV2I GAAGAC 1 cut(s) 192
BsuI GTATCC 1 cut(s) 905
BtsCI GGATG 4 cut(s) 143, 571, 688, 1110
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 3 cut(s) 368, 521, 748
CaiI CAGNNNCTG 1 cut(s) 105
CciI TCATGA 1 cut(s) 700
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 197, 644
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 5 cut(s) 154, 336, 605, 701, 1152
CviQI GTAC 2 cut(s) 197, 644
DdeI CTNAG 4 cut(s) 193, 210, 894, 1034
DpnI GATC 6 cut(s) 59, 303, 609, 699, 859, 1019
DpnII GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 1060
Eco130I CCWWGG 1 cut(s) 1095
Eco24I GRGCYC 1 cut(s) 1062
Eco47I GGWCC 1 cut(s) 487
Eco53kI GAGCTC 1 cut(s) 1060
Eco57I CTGAAG 1 cut(s) 497
EcoICRI GAGCTC 1 cut(s) 1060
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoRI GAATTC 1 cut(s) 751
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 1095
EcoT38I GRGCYC 1 cut(s) 1062
ErhI CCWWGG 1 cut(s) 1095
FaeI CATG 5 cut(s) 157, 339, 608, 704, 1155
FatI CATG 5 cut(s) 153, 335, 604, 700, 1151
FbaI TGATCA 3 cut(s) 607, 697, 1017
Fnu4HI GCNGC 3 cut(s) 86, 445, 1039
FokI GGATG 4 cut(s) 130, 578, 675, 1117
FriOI GRGCYC 1 cut(s) 1062
Fsp4HI GCNGC 3 cut(s) 86, 445, 1039
FspBI CTAG 4 cut(s) 26, 869, 908, 938
GluI GCNGC 3 cut(s) 86, 445, 1039
HapII CCGG 1 cut(s) 200
Hin1II CATG 5 cut(s) 157, 339, 608, 704, 1155
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 5 cut(s) 228, 265, 620, 874, 975
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 283, 793
Hpy166II GTNNAC 4 cut(s) 135, 285, 1048, 1075
Hpy188I TCNGA 9 cut(s) 57, 101, 169, 233, 250, 478, 532, 897, 1104
Hpy188III TCNNGA 8 cut(s) 26, 209, 262, 299, 309, 395, 701, 756
Hpy8I GTNNAC 4 cut(s) 135, 285, 1048, 1075
HpyAV CCTTC 3 cut(s) 338, 610, 1147
HpyCH4III ACNGT 2 cut(s) 822, 1067
HpyF3I CTNAG 4 cut(s) 193, 210, 894, 1034
Hsp92II CATG 5 cut(s) 157, 339, 608, 704, 1155
Ksp22I TGATCA 3 cut(s) 607, 697, 1017
Kzo9I GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
LmnI GCTCC 2 cut(s) 519, 1097
LpnPI CCDG 9 cut(s) 213, 222, 294, 596, 636, 663, 929, 1036, 1071
Lsp1109I GCAGC 2 cut(s) 431, 1050
LweI GCATC 2 cut(s) 590, 697
MaeI CTAG 4 cut(s) 26, 869, 908, 938
MaeIII GTNAC 1 cut(s) 353
MalI GATC 6 cut(s) 59, 303, 609, 699, 859, 1019
MboI GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
MboII GAAGA 4 cut(s) 44, 197, 796, 804
MhlI GDGCHC 1 cut(s) 1062
MluCI AATT 8 cut(s) 8, 399, 470, 736, 751, 864, 949, 964
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 4 cut(s) 211, 356, 716, 984
MnlI CCTC 8 cut(s) 88, 95, 299, 381, 741, 994, 1007, 1137
MroXI GAANNNNTTC 1 cut(s) 751
MseI TTAA 4 cut(s) 11, 45, 594, 723
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 539
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 378
NdeII GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
NlaIII CATG 5 cut(s) 157, 339, 608, 704, 1155
PagI TCATGA 1 cut(s) 700
PctI GAATGC 1 cut(s) 539
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 4 cut(s) 265, 620, 874, 975
PflMI CCANNNNNTGG 1 cut(s) 556
PfoI TCCNGGA 1 cut(s) 649
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 3 cut(s) 87, 446, 1040
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 1062
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspPI GGNCC 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 105
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 2 cut(s) 198, 645
RsaNI GTAC 2 cut(s) 197, 644
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 1062
SaqAI TTAA 4 cut(s) 11, 45, 594, 723
SatI GCNGC 3 cut(s) 86, 445, 1039
Sau3AI GATC 6 cut(s) 57, 301, 607, 697, 857, 1017
Sau96I GGNCC 1 cut(s) 487
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 651
SduI GDGCHC 1 cut(s) 1062
SetI ASST 8 cut(s) 45, 99, 216, 296, 411, 1062, 1087, 1139
SfaNI GCATC 2 cut(s) 590, 697
SinI GGWCC 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 92, 998
SmoI CTYRAG 2 cut(s) 92, 998
Sse9I AATT 8 cut(s) 8, 399, 470, 736, 751, 864, 949, 964
SsiI CCGC 1 cut(s) 85
SspI AATATT 1 cut(s) 661
SspMI CTAG 4 cut(s) 26, 869, 908, 938
SstI GAGCTC 1 cut(s) 1062
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 822, 1067
TaqI TCGA 3 cut(s) 630, 755, 856
TasI AATT 8 cut(s) 8, 399, 470, 736, 751, 864, 949, 964
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 4 cut(s) 265, 620, 874, 975
Tru1I TTAA 4 cut(s) 11, 45, 594, 723
Tru9I TTAA 4 cut(s) 11, 45, 594, 723
TscAI CASTG 3 cut(s) 368, 521, 748
TseI GCWGC 2 cut(s) 444, 1038
TspDTI ATGAA 3 cut(s) 198, 612, 946
TspRI CASTG 3 cut(s) 368, 521, 748
Van91I CCANNNNNTGG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 487
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 4 cut(s) 399, 470, 736, 751
XbaI TCTAGA 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 751
XspI CTAG 4 cut(s) 26, 869, 908, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.