Prupe.8G236800_v2.0.a1

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0012898
Physical Location & Seq
Forward (+)
20917941 .. 20921715
3775 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G236800.1

Sequence Viewer

Length: 1494 bp
ATGGGCCTCCGGTTCGCAGGCCCGTTAATCGTTCCCAAGCCCGCCCAATATAACTTAAAGAGACACGGCGTCGTTTTAGGTTGGTTTCTTCTTCGTTCAAAGAGTGGGAAAACGAGGGAGGGTTCCCAAATTCGCGGAAGTGCGGAGAGGTGTTTCGAATCGGTGGCGGCGAACGGCTCTCCCAACTCCACCCTTCCCCTGCTTACCGCCAGCCAACCCAAACAGAGAACCACCCCACCTTCACCCTCAGCCCCCAACCACTGCTCTGGTCCAAGAGATAGAGGACCAGTGGTTGGGTGGGGGGGAAGGTGGGTGGTCTGTCTGGGTTGGCTGGCGGTAAGCAGTGAGAGGGTGGAGTTGATCGGAGAGTGCTTAAGTCGGAGGATTGGGGTTGGCTGGAGAGTTTTCGAGTCTAGCTTTGTAGGAAGGAGGTCTATTGTCAAGGAACGCTTCTCAAAGAAATACAGGCACCCATCTTTGGATGCTAAGCTTACACTCAAGCGCCTCAATGCAGAGGCCAGGTGCATGACAAAAGCAAGACGACTTGGTGTCTATACTCCAGTGCTTTACTCTGTAGACATTGTGCTGCATACCCTAACATTTGAATATGTGGAGGGTCCTTCTGTCAAAGATATATTTCTTGAATTTGGGTTACGCAATGGCGCTGTTGTTGAAGAACGCATGGATGATATTGCAATGCAGATTGGTCATGCAATTGGAAAACTGCATGATGGTGGTCTCATTCATGGCGACCTGACCACATCTAACATGTTGATTAGAGCTGCTACCAACCAGTTGGTTGTTATTGATTTCGGTCTGAGCTTTACATCAACCCTTCCAGAAGATAAAGCTGTGGATTTGTATGTTTTGGAACGAGCTTTGCTCTCAATGCATTCTTCATGTGGGAATGTGATGGATCGGATACTTGCTGCTTATCGGAAGTCCTCAAAGCAGTGGTCGTCCACATTAAACAAGTTAGCTCAAGTGTTACAAATGGATCATTCACTATACAACACCCATGTAAAACTCTTAGCCTTCGACCTCCTCACTCTCACCCAAACCTCATCTTCCTCCTCTTCCTCAGACCCAGTTTCCTTCTCTCGCAATAGCGCTCTCCTTTCCCGCGCCGAGACCGTCGGCACCGTCACGTCCCGTGACCTCAAACCCAACAAGTTCCTCCGCTTTACTATCGACGACGGCACCGGCTGCGTCAACTGCATCCTCTGGCTCAACCAGCTCTCTTCCCCCTACTTTTCGCGCCGTAACCCGGCAGATGTTCGACTTATTGCCCAAACGGCGCAGCACTTCGCGGCGGAGATTAAGCTCGGAGTGGTTGCCAGGGTGCGCGGGAAGATCACGAGCTATAGAGGCGTTACGCAGATCACTGCGTTGGATGTTGTCGTTGAGAGGGACCCCAATGTGGAGATTTTGCATTGGTTGGATTGCATGAGGTTGGCGCGAAAAGTTTACAATGTCGTTCCTCCATGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

498

Amino Acids

55.08

Weight (kDa)

9.78

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26110 AT5G26110 AT5G26110
fragaria_vesca FvH4_2g23150
malus_domestica MD11G1280900.v1.1
prunus_persica Prupe.8G236800_v2.0.a1
pyrus_communis pycom11g24870
rosa_chinensis RchiOBHm_Chr6g0290401
rosa_laevigata RLG00000012222
rosa_multiflora Rmu_sc0029478.1_g000001
rosa_rugosa Rorug06G0212500 Rorug06G0212600
rosa_samantha Rh6AG322500 Rh6BG329900 Rh6CG335900 Rh6DG322800
rosa_wichuraiana Rw6G027860 Rw6G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 468, 1139, 1199
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 576
AccII CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
AclWI GGATC 2 cut(s) 924, 1005
AcsI RAATTY 2 cut(s) 129, 644
AcyI GRCGYC 1 cut(s) 69
AfeI AGCGCT 1 cut(s) 1111
AfiI CCNNNNNNNGG 4 cut(s) 293, 478, 1267, 1420
AflII CTTAAG 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 4 cut(s) 99, 605, 644, 674
AhdI GACNNNNNGTC 2 cut(s) 68, 548
AjiI CACGTC 1 cut(s) 1149
AjnI CCWGG 2 cut(s) 518, 1337
Alw26I GTCTC 3 cut(s) 55, 743, 1124
AlwI GGATC 2 cut(s) 924, 1005
Aor51HI AGCGCT 1 cut(s) 1111
AoxI GGCC 3 cut(s) 4, 19, 516
ApeKI GCWGC 5 cut(s) 586, 782, 929, 1206, 1300
ApoI RAATTY 2 cut(s) 129, 644
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AspLEI GCGC 8 cut(s) 504, 665, 1112, 1127, 1260, 1300, 1347, 1459
AspS9I GGNCC 6 cut(s) 4, 20, 269, 284, 617, 1411
AsuC2I CCSGG 1 cut(s) 1268
AsuHPI GGTGA 2 cut(s) 234, 1045
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 4 cut(s) 269, 284, 617, 1411
BanI GGYRCC 3 cut(s) 468, 1139, 1199
BauI CACGAG 1 cut(s) 1357
BbvCI CCTCAGC 1 cut(s) 247
BbvI GCAGC 5 cut(s) 573, 769, 916, 1193, 1312
BccI CCATC 3 cut(s) 481, 725, 907
BceAI ACGGC 5 cut(s) 82, 190, 1213, 1245, 1311
BciT130I CCWGG 2 cut(s) 520, 1339
BciVI GTATCC 1 cut(s) 915
BcnI CCSGG 1 cut(s) 1268
BcoDI GTCTC 3 cut(s) 55, 743, 1124
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 2 cut(s) 573, 1363
BfoI RGCGCY 3 cut(s) 505, 666, 1113
BfrI CTTAAG 1 cut(s) 373
BfuI GTATCC 1 cut(s) 915
BglI GCCNNNNNGGC 1 cut(s) 1295
BisI GCNGC 7 cut(s) 168, 587, 783, 930, 1207, 1301, 1311
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 7 cut(s) 169, 588, 784, 931, 1208, 1302, 1312
Bme1390I CCNGG 3 cut(s) 520, 1268, 1339
Bme18I GGWCC 4 cut(s) 269, 284, 617, 1411
BmeRI GACNNNNNGTC 2 cut(s) 68, 548
BmgBI CACGTC 1 cut(s) 1149
BmgT120I GGNCC 6 cut(s) 4, 20, 269, 284, 617, 1411
BmiI GGNNCC 7 cut(s) 124, 470, 618, 1141, 1201, 1412, 1413
BmrFI CCNGG 3 cut(s) 520, 1268, 1339
BmrI ACTGGG 1 cut(s) 1082
BmsI GCATC 2 cut(s) 472, 1227
BmuI ACTGGG 1 cut(s) 1082
BplI GAGNNNNNCTC 2 cut(s) 867, 899
BpmI CTGGAG 2 cut(s) 418, 543
Bpu10I CCTNAGC 1 cut(s) 247
Bpu1102I GCTNAGC 1 cut(s) 486
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 482, 966
BpuMI CCSGG 1 cut(s) 1268
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 2 cut(s) 743, 1124
BsaJI CCNNGG 1 cut(s) 1338
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 478, 1267, 1420
Bse118I RCCGGY 1 cut(s) 1202
Bse1I ACTGG 4 cut(s) 287, 560, 793, 1088
Bse3DI GCAATG 2 cut(s) 664, 702
BseBI CCWGG 2 cut(s) 520, 1339
BseDI CCNNGG 1 cut(s) 1338
BseGI GGATG 4 cut(s) 487, 691, 1218, 1399
BseLI CCNNNNNNNGG 4 cut(s) 293, 478, 1267, 1420
BseMI GCAATG 2 cut(s) 664, 702
BseMII CTCAG 3 cut(s) 261, 809, 1095
BseNI ACTGG 4 cut(s) 287, 560, 793, 1088
BseRI GAGGAG 2 cut(s) 1034, 1063
BseXI GCAGC 5 cut(s) 573, 769, 916, 1193, 1312
Bsh1236I CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
BshFI GGCC 3 cut(s) 6, 21, 518
BshNI GGYRCC 3 cut(s) 468, 1139, 1199
BsiSI CCGG 3 cut(s) 10, 1203, 1268
BslFI GGGAC 2 cut(s) 1135, 1424
BslI CCNNNNNNNGG 4 cut(s) 293, 478, 1267, 1420
BsmAI GTCTC 3 cut(s) 55, 743, 1124
BsmFI GGGAC 2 cut(s) 1135, 1424
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 3 cut(s) 6, 21, 518
Bso31I GGTCTC 2 cut(s) 743, 1124
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 5 cut(s) 360, 916, 997, 1353, 1380
Bsp1720I GCTNAGC 1 cut(s) 486
BspANI GGCC 3 cut(s) 6, 21, 518
BspCNI CTCAG 3 cut(s) 260, 810, 1094
BspFNI CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
BspLI GGNNCC 7 cut(s) 124, 470, 618, 1141, 1201, 1412, 1413
BspPI GGATC 2 cut(s) 924, 1005
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 3 cut(s) 468, 1139, 1199
BspTI CTTAAG 1 cut(s) 373
BspTNI GGTCTC 2 cut(s) 743, 1124
BsrDI GCAATG 2 cut(s) 664, 702
BsrFI RCCGGY 1 cut(s) 1202
BsrI ACTGG 4 cut(s) 287, 560, 793, 1088
BssAI RCCGGY 1 cut(s) 1202
BssECI CCNNGG 1 cut(s) 1338
BssMI GATC 5 cut(s) 360, 916, 997, 1353, 1380
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 1357
Bst2BI CACGAG 1 cut(s) 1357
Bst2UI CCWGG 2 cut(s) 520, 1339
Bst4CI ACNGT 2 cut(s) 1135, 1144
Bst6I CTCTTC 2 cut(s) 1081, 1246
BstACI GRCGYC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 373
BstAPI GCANNNNNTGC 1 cut(s) 1206
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 19, 42, 211, 333
BstDEI CTNAG 5 cut(s) 247, 486, 818, 1030, 1081
BstF5I GGATG 4 cut(s) 487, 691, 1218, 1399
BstFNI CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
BstH2I RGCGCY 3 cut(s) 505, 666, 1113
BstHHI GCGC 8 cut(s) 504, 665, 1112, 1127, 1260, 1300, 1347, 1459
BstKTI GATC 5 cut(s) 363, 919, 1000, 1356, 1383
BstMAI GTCTC 3 cut(s) 55, 743, 1124
BstMBI GATC 5 cut(s) 360, 916, 997, 1353, 1380
BstMWI GCNNNNNNNGC 6 cut(s) 889, 1206, 1215, 1234, 1295, 1368
BstNI CCWGG 2 cut(s) 520, 1339
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 3 cut(s) 518, 1266, 1337
BstSFI CTRYAG 2 cut(s) 573, 1363
BstUI CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
BstV1I GCAGC 5 cut(s) 573, 769, 916, 1193, 1312
BstXI CCANNNNNNTGG 2 cut(s) 266, 796
BsuI GTATCC 1 cut(s) 915
BsuRI GGCC 3 cut(s) 6, 21, 518
BtrI CACGTC 1 cut(s) 1149
BtsCI GGATG 4 cut(s) 487, 691, 1218, 1399
BtsI GCAGTG 4 cut(s) 259, 349, 959, 1383
BtsIMutI CAGTG 6 cut(s) 259, 294, 349, 567, 959, 1383
Cac8I GCNNGC 4 cut(s) 19, 42, 211, 333
CfoI GCGC 8 cut(s) 504, 665, 1112, 1127, 1260, 1300, 1347, 1459
Cfr10I RCCGGY 1 cut(s) 1202
Cfr13I GGNCC 6 cut(s) 4, 20, 269, 284, 617, 1411
CseI GACGC 2 cut(s) 58, 1198
DdeI CTNAG 5 cut(s) 247, 486, 818, 1030, 1081
DpnI GATC 5 cut(s) 362, 918, 999, 1355, 1382
DpnII GATC 5 cut(s) 360, 916, 997, 1353, 1380
DriI GACNNNNNGTC 2 cut(s) 68, 548
Eam1104I CTCTTC 2 cut(s) 1081, 1246
Eam1105I GACNNNNNGTC 2 cut(s) 68, 548
EarI CTCTTC 2 cut(s) 1081, 1246
EciI GGCGGA 1 cut(s) 1328
Eco31I GGTCTC 2 cut(s) 743, 1124
Eco47I GGWCC 4 cut(s) 269, 284, 617, 1411
Eco47III AGCGCT 1 cut(s) 1111
EcoO109I RGGNCCY 2 cut(s) 617, 1411
EcoRII CCWGG 2 cut(s) 518, 1337
EcoT22I ATGCAT 1 cut(s) 894
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 2 cut(s) 1135, 1424
FauI CCCGC 3 cut(s) 49, 1130, 1340
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 7 cut(s) 168, 587, 783, 930, 1207, 1301, 1311
FokI GGATG 4 cut(s) 494, 698, 1205, 1406
Fsp4HI GCNGC 7 cut(s) 168, 587, 783, 930, 1207, 1301, 1311
FspBI CTAG 1 cut(s) 414
GlaI GCGC 8 cut(s) 503, 664, 1111, 1126, 1259, 1299, 1346, 1458
GluI GCNGC 7 cut(s) 168, 587, 783, 930, 1207, 1301, 1311
GsuI CTGGAG 2 cut(s) 418, 543
HaeII RGCGCY 3 cut(s) 505, 666, 1113
HaeIII GGCC 3 cut(s) 6, 21, 518
HapII CCGG 3 cut(s) 10, 1203, 1268
HgaI GACGC 2 cut(s) 58, 1198
HhaI GCGC 8 cut(s) 504, 665, 1112, 1127, 1260, 1300, 1347, 1459
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 8 cut(s) 502, 663, 1110, 1125, 1258, 1298, 1345, 1457
HinP1I GCGC 8 cut(s) 502, 663, 1110, 1125, 1258, 1298, 1345, 1457
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 158, 410
HpaII CCGG 3 cut(s) 10, 1203, 1268
HphI GGTGA 2 cut(s) 234, 1045
Hpy166II GTNNAC 4 cut(s) 577, 963, 1213, 1468
Hpy188I TCNGA 7 cut(s) 365, 381, 819, 921, 939, 1084, 1328
Hpy188III TCNNGA 3 cut(s) 641, 839, 1357
Hpy8I GTNNAC 4 cut(s) 577, 963, 1213, 1468
Hpy99I CGWCG 4 cut(s) 74, 1139, 1196, 1199
HpyAV CCTTC 8 cut(s) 203, 249, 300, 420, 630, 845, 1045, 1105
HpyCH4III ACNGT 2 cut(s) 1135, 1144
HpyCH4IV ACGT 1 cut(s) 1148
HpyF10VI GCNNNNNNNGC 6 cut(s) 889, 1206, 1215, 1234, 1295, 1368
HpyF3I CTNAG 5 cut(s) 247, 486, 818, 1030, 1081
HpySE526I ACGT 1 cut(s) 1148
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 8 cut(s) 502, 663, 1110, 1125, 1258, 1298, 1345, 1457
KflI GGGWCCC 1 cut(s) 1411
Kzo9I GATC 5 cut(s) 360, 916, 997, 1353, 1380
Lsp1109I GCAGC 5 cut(s) 573, 769, 916, 1193, 1312
LweI GCATC 2 cut(s) 472, 1227
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 1148
MaeIII GTNAC 6 cut(s) 651, 987, 1144, 1154, 1262, 1372
MalI GATC 5 cut(s) 362, 918, 999, 1355, 1382
MboI GATC 5 cut(s) 360, 916, 997, 1353, 1380
MboII GAAGA 9 cut(s) 80, 83, 686, 854, 888, 1059, 1068, 1233, 1363
MfeI CAATTG 1 cut(s) 714
MluCI AATT 3 cut(s) 129, 644, 714
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 3 cut(s) 359, 1371, 1419
Mph1103I ATGCAT 1 cut(s) 894
MseI TTAA 5 cut(s) 26, 56, 374, 968, 1320
MslI CAYNNNNRTG 1 cut(s) 732
MspCI CTTAAG 1 cut(s) 373
MspI CCGG 3 cut(s) 10, 1203, 1268
MspR9I CCNGG 3 cut(s) 520, 1268, 1339
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 2 cut(s) 520, 1339
MvnI CGCG 6 cut(s) 135, 1125, 1258, 1310, 1347, 1459
MwoI GCNNNNNNNGC 6 cut(s) 889, 1206, 1215, 1234, 1295, 1368
NciI CCSGG 1 cut(s) 1268
NdeII GATC 5 cut(s) 360, 916, 997, 1353, 1380
NlaIV GGNNCC 7 cut(s) 124, 470, 618, 1141, 1201, 1412, 1413
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 2 cut(s) 1144, 1154
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 156
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 1 cut(s) 158
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 7 cut(s) 169, 588, 784, 931, 1208, 1302, 1312
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PpuMI RGGWCCY 2 cut(s) 617, 1411
PscI ACATGT 1 cut(s) 768
Psp5II RGGWCCY 2 cut(s) 617, 1411
Psp6I CCWGG 2 cut(s) 518, 1337
PspGI CCWGG 2 cut(s) 518, 1337
PspN4I GGNNCC 7 cut(s) 124, 470, 618, 1141, 1201, 1412, 1413
PspPI GGNCC 6 cut(s) 4, 20, 269, 284, 617, 1411
PspPPI RGGWCCY 2 cut(s) 617, 1411
RseI CAYNNNNRTG 1 cut(s) 732
SaqAI TTAA 5 cut(s) 26, 56, 374, 968, 1320
SatI GCNGC 7 cut(s) 168, 587, 783, 930, 1207, 1301, 1311
Sau3AI GATC 5 cut(s) 360, 916, 997, 1353, 1380
Sau96I GGNCC 6 cut(s) 4, 20, 269, 284, 617, 1411
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 3 cut(s) 520, 1268, 1339
SfaNI GCATC 2 cut(s) 472, 1227
SfcI CTRYAG 2 cut(s) 573, 1363
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 4 cut(s) 269, 284, 617, 1411
SmiMI CAYNNNNRTG 1 cut(s) 732
SmlI CTYRAG 3 cut(s) 373, 497, 981
SmoI CTYRAG 3 cut(s) 373, 497, 981
Sse9I AATT 3 cut(s) 129, 644, 714
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 518, 1266, 1337
TaaI ACNGT 2 cut(s) 1135, 1144
TaiI ACGT 1 cut(s) 1151
TaqI TCGA 5 cut(s) 156, 408, 1038, 1191, 1279
TaqII GACCGA 1 cut(s) 803
TasI AATT 3 cut(s) 129, 644, 714
TauI GCSGC 2 cut(s) 170, 1313
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 5 cut(s) 26, 56, 374, 968, 1320
Tru9I TTAA 5 cut(s) 26, 56, 374, 968, 1320
TscAI CASTG 6 cut(s) 266, 294, 349, 567, 959, 1390
TseFI GTSAC 2 cut(s) 1144, 1154
TseI GCWGC 5 cut(s) 586, 782, 929, 1206, 1300
Tsp45I GTSAC 2 cut(s) 1144, 1154
TspDTI ATGAA 2 cut(s) 734, 888
TspRI CASTG 6 cut(s) 266, 294, 349, 567, 959, 1390
Van91I CCANNNNNTGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 373
VpaK11BI GGWCC 4 cut(s) 269, 284, 617, 1411
XapI RAATTY 2 cut(s) 129, 644
XceI RCATGY 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.