FvH4_2g26081

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017131
Physical Location & Seq
Reverse (-)
20990188 .. 20990733
546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26081.t1

Sequence Viewer

Length: 546 bp
ATGATGCTGCCTGGCTTCATATTTTGTGCTGCAGTCCTTTTGGCAACACTCACTGTGATTCTCCTGGCTTTAGTTTCTCCAGTACCCGAAAGAAAGCCCTCGCCGCCTTGGCTTGACCTTTCCTTGTACATCCAGCAGCCACACATTGCAAGCTCCAATGCACATCAGCCGGTGGCACAAGCAGACACCGGAGCATTCATCTTCCACCGCAAGCTCACGGAGGGACCCGAGAACACTTCTCGGGTAGTTGGAAAAGCTCAAGGTTTCATTATCCCTATGGAAGACTTCGCACAGTCGGCATTCAATATCATCTACCTCACATTTCACACGCCTGAGTATTCCGGTAGCCTTAGCGTTCAGGCCAAGCATGTTTCCCACAAAGACAGAGAAGAACTCACCGTGATGGGTGGAACAGGATCGTTTGCTTTTGCTCGCGGACTAGCTGATTTTGCTCAGAGTGATCAGCAGTCGCCCGGTGCCGAATCCACTTACCATGTCAAGCTGCAGCTTAGATTTCCTAATCAGTCTCGCACTACTCTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

182

Amino Acids

19.77

Weight (kDa)

6.64

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 65 - 166 4e-19 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42655 AT5G42655
fragaria_vesca FvH4_2g26081
malus_domestica MD03G1292000.v1.1 MD11G1306400.v1.1
prunus_persica Prupe.8G265000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294451
rosa_laevigata RLG00000011868
rosa_roxburghii Rroxscaffold_7G00173320
rosa_samantha Rh6AG357300 Rh6BG364200 Rh6CG371300 Rh6DG358100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccII CGCG 1 cut(s) 435
AciI CCGC 3 cut(s) 104, 208, 435
AclWI GGATC 1 cut(s) 424
AfaI GTAC 2 cut(s) 84, 128
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 2 cut(s) 10, 63
AluBI AGCT 6 cut(s) 153, 214, 257, 443, 502, 508
AluI AGCT 6 cut(s) 153, 214, 257, 443, 502, 508
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 1 cut(s) 424
Ama87I CYCGRG 2 cut(s) 227, 240
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 5 cut(s) 7, 29, 136, 502, 505
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 2 cut(s) 227, 240
AvaII GGWCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 16, 148, 489, 517
BccI CCATC 1 cut(s) 397
BciT130I CCWGG 2 cut(s) 12, 65
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 2 cut(s) 440, 539
BfmI CTRYAG 2 cut(s) 30, 503
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 6 cut(s) 8, 30, 104, 137, 503, 506
BlsI GCNGC 6 cut(s) 9, 31, 105, 138, 504, 507
Bme1390I CCNGG 3 cut(s) 12, 65, 474
Bme18I GGWCC 1 cut(s) 224
BmeT110I CYCGRG 2 cut(s) 227, 240
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 3 cut(s) 225, 226, 478
BmrFI CCNGG 3 cut(s) 12, 65, 474
BpiI GAAGAC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 378, 410
BpmI CTGGAG 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 107
BsaWI WCCGGW 2 cut(s) 188, 341
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 2 cut(s) 12, 65
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 324, 467
BseNI ACTGG 1 cut(s) 80
BseXI GCAGC 4 cut(s) 16, 148, 489, 517
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 1 cut(s) 476
BsiHKCI CYCGRG 2 cut(s) 227, 240
BsiSI CCGG 4 cut(s) 170, 189, 342, 474
BslFI GGGAC 1 cut(s) 237
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 237
BsmI GAATGC 2 cut(s) 194, 299
BsnI GGCC 1 cut(s) 362
BsoBI CYCGRG 2 cut(s) 227, 240
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 416, 460
BspACI CCGC 3 cut(s) 104, 208, 435
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 2 cut(s) 325, 466
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 225, 226, 478
BspMAI CTGCAG 2 cut(s) 34, 507
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 2 cut(s) 416, 460
BssT1I CCWWGG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 12, 65
Bst4CI ACNGT 3 cut(s) 55, 294, 400
BstAUI TGTACA 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 151, 212, 433
BstDEI CTNAG 4 cut(s) 333, 350, 453, 509
BstF5I GGATG 1 cut(s) 129
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 2 cut(s) 419, 463
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 2 cut(s) 416, 460
BstMWI GCNNNNNNNGC 4 cut(s) 103, 109, 296, 449
BstNI CCWGG 2 cut(s) 12, 65
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 3 cut(s) 10, 63, 472
BstSFI CTRYAG 2 cut(s) 30, 503
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 4 cut(s) 16, 148, 489, 517
BstV2I GAAGAC 1 cut(s) 288
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 3 cut(s) 151, 212, 433
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 224
Csp6I GTAC 2 cut(s) 83, 127
CviAII CATG 2 cut(s) 368, 494
CviQI GTAC 2 cut(s) 83, 127
DdeI CTNAG 4 cut(s) 333, 350, 453, 509
DpnI GATC 2 cut(s) 418, 462
DpnII GATC 2 cut(s) 416, 460
Eco130I CCWWGG 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 224
Eco88I CYCGRG 2 cut(s) 227, 240
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 10, 63
EcoT14I CCWWGG 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 371, 497
FaiI YATR 4 cut(s) 20, 278, 369, 495
FaqI GGGAC 1 cut(s) 237
FatI CATG 2 cut(s) 367, 493
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 6 cut(s) 8, 30, 104, 137, 503, 506
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 6 cut(s) 8, 30, 104, 137, 503, 506
FspBI CTAG 2 cut(s) 440, 539
GluI GCNGC 6 cut(s) 8, 30, 104, 137, 503, 506
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 362
HapII CCGG 4 cut(s) 170, 189, 342, 474
Hin1II CATG 2 cut(s) 371, 497
HinfI GANTC 2 cut(s) 58, 482
HpaII CCGG 4 cut(s) 170, 189, 342, 474
HphI GGTGA 1 cut(s) 388
Hpy188I TCNGA 1 cut(s) 456
HpyCH4III ACNGT 3 cut(s) 55, 294, 400
HpyCH4V TGCA 4 cut(s) 32, 149, 161, 505
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 109, 296, 449
HpyF3I CTNAG 4 cut(s) 333, 350, 453, 509
Hsp92II CATG 2 cut(s) 371, 497
KflI GGGWCCC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 416, 460
LmnI GCTCC 2 cut(s) 158, 191
Lsp1109I GCAGC 4 cut(s) 16, 148, 489, 517
MaeI CTAG 2 cut(s) 440, 539
MalI GATC 2 cut(s) 418, 462
MboI GATC 2 cut(s) 416, 460
MboII GAAGA 3 cut(s) 193, 293, 401
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 3 cut(s) 109, 214, 326
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 4 cut(s) 170, 189, 342, 474
MspR9I CCNGG 3 cut(s) 12, 65, 474
Mva1269I GAATGC 2 cut(s) 194, 299
MvaI CCWGG 2 cut(s) 12, 65
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 103, 109, 296, 449
NciI CCSGG 1 cut(s) 474
NdeII GATC 2 cut(s) 416, 460
NlaIII CATG 2 cut(s) 371, 497
NlaIV GGNNCC 3 cut(s) 225, 226, 478
NspI RCATGY 1 cut(s) 371
PctI GAATGC 2 cut(s) 194, 299
PfeI GAWTC 2 cut(s) 58, 482
PkrI GCNGC 6 cut(s) 9, 31, 105, 138, 504, 507
PpuMI RGGWCCY 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 10, 63
PspGI CCWGG 2 cut(s) 10, 63
PspN4I GGNNCC 3 cut(s) 225, 226, 478
PspPI GGNCC 1 cut(s) 224
PspPPI RGGWCCY 1 cut(s) 224
PstI CTGCAG 2 cut(s) 34, 507
RsaI GTAC 2 cut(s) 84, 128
RsaNI GTAC 2 cut(s) 83, 127
RseI CAYNNNNRTG 1 cut(s) 401
SatI GCNGC 6 cut(s) 8, 30, 104, 137, 503, 506
Sau3AI GATC 2 cut(s) 416, 460
Sau96I GGNCC 1 cut(s) 224
ScrFI CCNGG 3 cut(s) 12, 65, 474
SetI ASST 9 cut(s) 120, 155, 216, 259, 265, 318, 445, 504, 510
SfcI CTRYAG 2 cut(s) 30, 503
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
SsiI CCGC 3 cut(s) 104, 208, 435
SspMI CTAG 2 cut(s) 440, 539
StyD4I CCNGG 3 cut(s) 10, 63, 472
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 3 cut(s) 55, 294, 400
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 58, 482
TscAI CASTG 1 cut(s) 58
TseI GCWGC 5 cut(s) 7, 29, 136, 502, 505
TspDTI ATGAA 3 cut(s) 7, 187, 256
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 224
XceI RCATGY 1 cut(s) 371
XspI CTAG 2 cut(s) 440, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.