MD03G1292000.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017131
Physical Location & Seq
Reverse (-)
36922633 .. 36923223
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1292000.v1.1.491

Sequence Viewer

Length: 591 bp
ATGTTGTCCAGGACCATTTTCTGCACGGCAGTCCTGTTGGCGACGCTGGCAGTAATCCTCTTGGCCTTGTTCTCGCAACTACCCAAAAGAAAGCCGTCGCCGCCGGGGCTCGACCTTTCATTGTACATCCAGCAACCAAACAACAACAACATTGCAGGCTCCAATAACGCGGTAGCACAACCACAACCCGATGGCGGCGGAGGAGCTTTCATCTTCCACCGGATGCTCACCGAGGGACCCGAGAACACTTCACGGGTGGTGGGAAAAGCGCAGCGCTTCATAATCCCTGCGGAGCACTTTGCAAAATCGGGATTCAATATAATCTATCTGACCTTCTACACGCCTCAGTTTTCGGGCAGCCTTAGCGTGGAGGCCAAGCATGTTTCCCGCCAAGACACGGAGGAGCTTACGGTGATGGGGGGAACGGGGTCGTTTGCTTTTGCGAGAGGAGTTTCTGTTTTTGCTAAGACGACTGATGAGGACGGTCACGTCGATCAGCAATCGTCGTCGGGGAATGTAGTGGATTATGCCACTTATCATGTGAAGTTGCAGCTTAGATTTCCCAATCAGTCTCAGACTGTTCTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.21

Weight (kDa)

7.91

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 46 - 159 5.7e-17 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42655 AT5G42655
fragaria_vesca FvH4_2g26081
malus_domestica MD03G1292000.v1.1 MD11G1306400.v1.1
prunus_persica Prupe.8G265000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294451
rosa_laevigata RLG00000011868
rosa_roxburghii Rroxscaffold_7G00173320
rosa_samantha Rh6AG357300 Rh6BG364200 Rh6CG371300 Rh6DG358100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 488
AccII CGCG 1 cut(s) 170
AciI CCGC 6 cut(s) 101, 170, 195, 198, 290, 388
AfaI GTAC 1 cut(s) 125
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 194, 367, 397
AgsI TTSAA 1 cut(s) 316
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 3 cut(s) 206, 406, 553
AluI AGCT 3 cut(s) 206, 406, 553
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 1 cut(s) 576
Ama87I CYCGRG 1 cut(s) 239
Aor51HI AGCGCT 1 cut(s) 275
AoxI GGCC 2 cut(s) 63, 372
ApeKI GCWGC 3 cut(s) 271, 357, 550
AspLEI GCGC 2 cut(s) 271, 276
AspS9I GGNCC 2 cut(s) 12, 236
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 220, 424
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 2 cut(s) 12, 236
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 283, 369, 562
BccI CCATC 2 cut(s) 185, 409
BceAI ACGGC 2 cut(s) 42, 79
BciT130I CCWGG 1 cut(s) 10
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 277
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 5 cut(s) 101, 196, 272, 358, 551
BlsI GCNGC 5 cut(s) 102, 197, 273, 359, 552
Bme1390I CCNGG 2 cut(s) 10, 105
Bme18I GGWCC 2 cut(s) 12, 236
BmeT110I CYCGRG 1 cut(s) 239
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 12, 236
BmiI GGNNCC 3 cut(s) 160, 237, 238
BmrFI CCNGG 2 cut(s) 10, 105
BmsI GCATC 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 104, 231
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 367, 397
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 2 cut(s) 104, 231
BseGI GGATG 2 cut(s) 126, 228
BseLI CCNNNNNNNGG 3 cut(s) 194, 367, 397
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 359, 587
BseRI GAGGAG 3 cut(s) 216, 416, 462
BseXI GCAGC 3 cut(s) 283, 369, 562
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 65, 374
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 2 cut(s) 104, 220
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 3 cut(s) 194, 367, 397
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 2 cut(s) 65, 374
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 111, 297
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 6 cut(s) 101, 170, 195, 198, 290, 388
BspANI GGCC 2 cut(s) 65, 374
BspCNI CTCAG 2 cut(s) 358, 586
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 3 cut(s) 160, 237, 238
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 104, 231
BssMI GATC 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 412, 485, 580
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 48, 157
BstDEI CTNAG 5 cut(s) 345, 362, 465, 554, 573
BstF5I GGATG 2 cut(s) 126, 228
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 2 cut(s) 271, 276
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 4 cut(s) 47, 100, 106, 363
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 383
BstSCI CCNGG 2 cut(s) 8, 103
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 3 cut(s) 283, 369, 562
BsuRI GGCC 2 cut(s) 65, 374
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 2 cut(s) 126, 228
Cac8I GCNNGC 2 cut(s) 48, 157
CfoI GCGC 2 cut(s) 271, 276
Cfr13I GGNCC 2 cut(s) 12, 236
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 124
CviAII CATG 2 cut(s) 380, 539
CviJI RGCY 9 cut(s) 65, 94, 109, 159, 206, 360, 374, 406, 553
CviKI_1 RGCY 9 cut(s) 65, 94, 109, 159, 206, 360, 374, 406, 553
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 5 cut(s) 345, 362, 465, 554, 573
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
DrdI GACNNNNNNGTC 1 cut(s) 488
DseDI GACNNNNNNGTC 1 cut(s) 488
EciI GGCGGA 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 12, 236
Eco47III AGCGCT 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 8
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 2 cut(s) 383, 542
FaiI YATR 5 cut(s) 281, 320, 381, 528, 540
FaqI GGGAC 1 cut(s) 249
FatI CATG 2 cut(s) 379, 538
FauI CCCGC 1 cut(s) 395
Fnu4HI GCNGC 5 cut(s) 101, 196, 272, 358, 551
FokI GGATG 2 cut(s) 113, 235
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 5 cut(s) 101, 196, 272, 358, 551
GlaI GCGC 2 cut(s) 270, 275
GluI GCNGC 5 cut(s) 101, 196, 272, 358, 551
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 2 cut(s) 65, 374
HapII CCGG 2 cut(s) 104, 220
HgaI GACGC 1 cut(s) 52
HhaI GCGC 2 cut(s) 271, 276
Hin1II CATG 2 cut(s) 383, 542
Hin6I GCGC 2 cut(s) 269, 274
HinP1I GCGC 2 cut(s) 269, 274
HinfI GANTC 1 cut(s) 312
HpaII CCGG 2 cut(s) 104, 220
HphI GGTGA 2 cut(s) 220, 424
Hpy188I TCNGA 2 cut(s) 330, 576
Hpy188III TCNNGA 1 cut(s) 309
Hpy99I CGWCG 5 cut(s) 46, 100, 494, 508, 511
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 3 cut(s) 412, 485, 580
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 4 cut(s) 24, 155, 302, 550
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 100, 106, 363
HpyF3I CTNAG 5 cut(s) 345, 362, 465, 554, 573
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 2 cut(s) 383, 542
HspAI GCGC 2 cut(s) 269, 274
KflI GGGWCCC 1 cut(s) 236
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 4 cut(s) 164, 203, 292, 403
LpnPI CCDG 9 cut(s) 22, 32, 47, 117, 141, 143, 233, 300, 569
Lsp1109I GCAGC 3 cut(s) 283, 369, 562
LweI GCATC 1 cut(s) 213
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 1 cut(s) 485
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 111, 297
MnlI CCTC 8 cut(s) 68, 194, 226, 354, 364, 394, 440, 472
MspI CCGG 2 cut(s) 104, 220
MspR9I CCNGG 2 cut(s) 10, 105
MteI GCGCNGCGC 1 cut(s) 272
MvaI CCWGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 4 cut(s) 47, 100, 106, 363
NciI CCSGG 1 cut(s) 105
NdeII GATC 1 cut(s) 493
NlaIII CATG 2 cut(s) 383, 542
NlaIV GGNNCC 3 cut(s) 160, 237, 238
NmuCI GTSAC 1 cut(s) 485
NspI RCATGY 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PfeI GAWTC 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 5 cut(s) 102, 197, 273, 359, 552
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 160, 237, 238
PspPI GGNCC 2 cut(s) 12, 236
PspPPI RGGWCCY 1 cut(s) 236
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 5 cut(s) 101, 196, 272, 358, 551
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 2 cut(s) 12, 236
ScrFI CCNGG 2 cut(s) 10, 105
SduI GDGCHC 2 cut(s) 111, 297
SetI ASST 6 cut(s) 117, 208, 335, 408, 492, 555
SfaNI GCATC 1 cut(s) 213
SinI GGWCC 2 cut(s) 12, 236
SsiI CCGC 6 cut(s) 101, 170, 195, 198, 290, 388
StyD4I CCNGG 2 cut(s) 8, 103
TaaI ACNGT 3 cut(s) 412, 485, 580
TaiI ACGT 1 cut(s) 492
TaqI TCGA 2 cut(s) 111, 492
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 103, 198
TfiI GAWTC 1 cut(s) 312
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 3 cut(s) 271, 357, 550
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 3 cut(s) 108, 199, 268
TspGWI ACGGA 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 12, 236
XceI RCATGY 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.