Rroxscaffold_7G00173320

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017131
Physical Location & Seq
Forward (+)
13375128 .. 13375673
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173320.1

Sequence Viewer

Length: 546 bp
ATGATGCTGGCTAGGATCATATTTTGCGCTGCAGTCCTTTTGGCAACACTCGCTGTGATTCTTCTGGCTTTGGTTTCTCCAGTACCCGAAAGAAAGCCATTACAGCCTTGGCTGGACCTTTCCTTGTACATCCAGCAGCCACACATTGCAAGCTCCAATGCGCATCAATCGGTGGCACAATCAGACCCCGTGGCATTCATCTTCCACCGCAAGCTCACGGAGGGACCCGAGAACACCTCTCGGGTAGTCGGAAAAGCCCAAGGTTTCATTATCCCTATGGAAGACTTTGCACAATCAGCATTCAATATTATCTACCTAACCTTTCACACGCCTGAGTATTCCGGCAGCCTTAGCGTTCAGGCCAAGCATGTATCCCGCAAAGACACAGAAGAGCTCACTGTGATGGGTGGAACAGGATCATTTGCTTTTGCTCGGGGACTGGCTGTCTTTGCTCAGACAGATCAGCAATCGTCGGGTGTCGAATCCACTTATCATGTAAAGCTGCAGCTTAGATTTCCTAATCAGTCTCGGACTACTGTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

181

Amino Acids

19.85

Weight (kDa)

7.95

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 65 - 167 1.7e-19 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42655 AT5G42655
fragaria_vesca FvH4_2g26081
malus_domestica MD03G1292000.v1.1 MD11G1306400.v1.1
prunus_persica Prupe.8G265000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294451
rosa_laevigata RLG00000011868
rosa_roxburghii Rroxscaffold_7G00173320
rosa_samantha Rh6AG357300 Rh6BG364200 Rh6CG371300 Rh6DG358100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 162
AciI CCGC 2 cut(s) 208, 376
AclWI GGATC 2 cut(s) 23, 424
AfaI GTAC 2 cut(s) 84, 128
AgsI TTSAA 1 cut(s) 304
AhdI GACNNNNNGTC 1 cut(s) 443
AluBI AGCT 5 cut(s) 153, 214, 394, 502, 508
AluI AGCT 5 cut(s) 153, 214, 394, 502, 508
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 2 cut(s) 23, 424
Ama87I CYCGRG 3 cut(s) 227, 240, 432
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 5 cut(s) 29, 136, 345, 502, 505
AspLEI GCGC 2 cut(s) 29, 163
AspS9I GGNCC 2 cut(s) 115, 224
AvaI CYCGRG 3 cut(s) 227, 240, 432
AvaII GGWCC 2 cut(s) 115, 224
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 5 cut(s) 16, 148, 357, 489, 517
BccI CCATC 1 cut(s) 397
BciVI GTATCC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 3 cut(s) 30, 503, 537
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 5 cut(s) 30, 137, 346, 503, 506
BlsI GCNGC 5 cut(s) 31, 138, 347, 504, 507
Bme18I GGWCC 2 cut(s) 115, 224
BmeRI GACNNNNNGTC 1 cut(s) 443
BmeT110I CYCGRG 3 cut(s) 227, 240, 432
BmgT120I GGNCC 2 cut(s) 115, 224
BmiI GGNNCC 2 cut(s) 225, 226
BmsI GCATC 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 107, 189, 259
Bse1I ACTGG 2 cut(s) 80, 444
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 107, 189, 259
BseGI GGATG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 324, 467
BseNI ACTGG 2 cut(s) 80, 444
BseXI GCAGC 5 cut(s) 16, 148, 357, 489, 517
BshFI GGCC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 3 cut(s) 227, 240, 432
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 2 cut(s) 237, 450
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 2 cut(s) 237, 450
BsmI GAATGC 2 cut(s) 194, 299
BsnI GGCC 1 cut(s) 362
BsoBI CYCGRG 3 cut(s) 227, 240, 432
Bsp1286I GDGCHC 1 cut(s) 396
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 3 cut(s) 15, 416, 460
BspACI CCGC 2 cut(s) 208, 376
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 2 cut(s) 325, 466
BspLI GGNNCC 2 cut(s) 225, 226
BspMAI CTGCAG 2 cut(s) 34, 507
BspPI GGATC 2 cut(s) 23, 424
BspQI GCTCTTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 2 cut(s) 80, 444
BssECI CCNNGG 3 cut(s) 107, 189, 259
BssMI GATC 3 cut(s) 15, 416, 460
BssT1I CCWWGG 2 cut(s) 107, 259
Bst4CI ACNGT 2 cut(s) 400, 538
Bst6I CTCTTC 1 cut(s) 384
BstAUI TGTACA 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 9, 151, 212
BstDEI CTNAG 4 cut(s) 333, 350, 453, 509
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 29, 163
BstKTI GATC 3 cut(s) 18, 419, 463
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 3 cut(s) 15, 416, 460
BstMWI GCNNNNNNNGC 5 cut(s) 50, 103, 296, 351, 449
BstNSI RCATGY 1 cut(s) 371
BstSFI CTRYAG 3 cut(s) 30, 503, 537
BstV1I GCAGC 5 cut(s) 16, 148, 357, 489, 517
BstV2I GAAGAC 1 cut(s) 288
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 3 cut(s) 9, 151, 212
CfoI GCGC 2 cut(s) 29, 163
Cfr13I GGNCC 2 cut(s) 115, 224
Csp6I GTAC 2 cut(s) 83, 127
CviAII CATG 2 cut(s) 368, 494
CviQI GTAC 2 cut(s) 83, 127
DdeI CTNAG 4 cut(s) 333, 350, 453, 509
DpnI GATC 3 cut(s) 17, 418, 462
DpnII GATC 3 cut(s) 15, 416, 460
DriI GACNNNNNGTC 1 cut(s) 443
Eam1104I CTCTTC 1 cut(s) 384
Eam1105I GACNNNNNGTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 384
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 107, 259
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 2 cut(s) 115, 224
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 3 cut(s) 227, 240, 432
EcoICRI GAGCTC 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 224
EcoT14I CCWWGG 2 cut(s) 107, 259
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 2 cut(s) 107, 259
FaeI CATG 2 cut(s) 371, 497
FaiI YATR 4 cut(s) 20, 278, 369, 495
FaqI GGGAC 2 cut(s) 237, 450
FatI CATG 2 cut(s) 367, 493
FauI CCCGC 1 cut(s) 383
Fnu4HI GCNGC 5 cut(s) 30, 137, 346, 503, 506
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 5 cut(s) 30, 137, 346, 503, 506
FspAI RTGCGCAY 1 cut(s) 162
FspBI CTAG 1 cut(s) 12
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 2 cut(s) 28, 162
GluI GCNGC 5 cut(s) 30, 137, 346, 503, 506
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 362
HapII CCGG 1 cut(s) 342
HhaI GCGC 2 cut(s) 29, 163
Hin1II CATG 2 cut(s) 371, 497
Hin6I GCGC 2 cut(s) 27, 161
HinP1I GCGC 2 cut(s) 27, 161
HinfI GANTC 2 cut(s) 58, 482
HpaII CCGG 1 cut(s) 342
Hpy188I TCNGA 4 cut(s) 184, 251, 456, 531
Hpy99I CGWCG 1 cut(s) 475
HpyCH4III ACNGT 2 cut(s) 400, 538
HpyCH4V TGCA 4 cut(s) 32, 149, 290, 505
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 103, 296, 351, 449
HpyF3I CTNAG 4 cut(s) 333, 350, 453, 509
Hsp92II CATG 2 cut(s) 371, 497
HspAI GCGC 2 cut(s) 27, 161
KflI GGGWCCC 1 cut(s) 224
Kzo9I GATC 3 cut(s) 15, 416, 460
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 9 cut(s) 50, 93, 98, 146, 344, 345, 355, 399, 425
Lsp1109I GCAGC 5 cut(s) 16, 148, 357, 489, 517
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 12
MalI GATC 3 cut(s) 17, 418, 462
MboI GATC 3 cut(s) 15, 416, 460
MboII GAAGA 4 cut(s) 53, 193, 293, 401
MhlI GDGCHC 1 cut(s) 396
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 2 cut(s) 214, 247
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 1 cut(s) 342
Mva1269I GAATGC 2 cut(s) 194, 299
MwoI GCNNNNNNNGC 5 cut(s) 50, 103, 296, 351, 449
NdeII GATC 3 cut(s) 15, 416, 460
NlaIII CATG 2 cut(s) 371, 497
NlaIV GGNNCC 2 cut(s) 225, 226
NsbI TGCGCA 1 cut(s) 162
NspI RCATGY 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 384
PctI GAATGC 2 cut(s) 194, 299
PfeI GAWTC 2 cut(s) 58, 482
PkrI GCNGC 5 cut(s) 31, 138, 347, 504, 507
PpuMI RGGWCCY 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 224
PspN4I GGNNCC 2 cut(s) 225, 226
PspPI GGNCC 2 cut(s) 115, 224
PspPPI RGGWCCY 1 cut(s) 224
PstI CTGCAG 2 cut(s) 34, 507
RsaI GTAC 2 cut(s) 84, 128
RsaNI GTAC 2 cut(s) 83, 127
RseI CAYNNNNRTG 1 cut(s) 401
SacI GAGCTC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 384
SatI GCNGC 5 cut(s) 30, 137, 346, 503, 506
Sau3AI GATC 3 cut(s) 15, 416, 460
Sau96I GGNCC 2 cut(s) 115, 224
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 3 cut(s) 30, 503, 537
SinI GGWCC 2 cut(s) 115, 224
SmiMI CAYNNNNRTG 1 cut(s) 401
SsiI CCGC 2 cut(s) 208, 376
SspI AATATT 1 cut(s) 307
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 396
StyI CCWWGG 2 cut(s) 107, 259
TaaI ACNGT 2 cut(s) 400, 538
TaqI TCGA 1 cut(s) 480
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 2 cut(s) 58, 482
TscAI CASTG 1 cut(s) 403
TseI GCWGC 5 cut(s) 29, 136, 345, 502, 505
TspDTI ATGAA 2 cut(s) 187, 256
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 115, 224
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 105
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.