MD11G1306400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017131
Physical Location & Seq
Forward (+)
42073015 .. 42073605
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1306400.v1.1.491

Sequence Viewer

Length: 591 bp
ATGCTGTCCAGGATCATTTTCTGCACGGCAGTCCTGTTGGCGACGTTGGCAGTAATCCTCTTGGCTTTGTTCTCGCCGCTACCTAAAAGAAAGTCGTCGCCGCCGTGGCTCGACCTTTCCTTATACATTCAACAACCACCCAACAACAACAACAACAACATTGCAGGCTCAAATAACGCCGGAAAACAACCGCAACCGGACAGCAGCGGAGGAGCGTTCATCTTCCACCGGATGCTCACCGAGGGACCCGAGAACACTTCCCGTGTGGTGGGGAAAGCACAGGGATTCATAATCCCCGTGGAGCACTTTGCCAACTCGGGATTCAACATAATCTACCTGACCTTCCACACGCCTCAGTATTCCGGCAGCCTCAGCGTGGAGGCCAAGCATGTTTCCCGCCAAGACACGGAGGAGCTTACGGTGATGGGCGGAACGGGGTCGTTTGCTTTTGCGAGAGGAGTTGCCGTTTTTGCTCAGACAACTGATCAGGATGACCAGCAACCGTCATCGGGGAATGGGGTGGATTATGCCACTTATCATGTAAAGTTGCAGCTTAGATTTCCCAACCAGTCTCGGACAGTTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.33

Weight (kDa)

7.92

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 164 4.7e-21 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42655 AT5G42655
fragaria_vesca FvH4_2g26081
malus_domestica MD03G1292000.v1.1 MD11G1306400.v1.1
prunus_persica Prupe.8G265000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294451
rosa_laevigata RLG00000011868
rosa_roxburghii Rroxscaffold_7G00173320
rosa_samantha Rh6AG357300 Rh6BG364200 Rh6CG371300 Rh6DG358100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 77, 101, 191, 207, 397, 429
AclWI GGATC 1 cut(s) 20
AfiI CCNNNNNNNGG 4 cut(s) 268, 376, 406, 509
AgsI TTSAA 2 cut(s) 131, 325
AjnI CCWGG 1 cut(s) 8
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 2 cut(s) 415, 553
AluI AGCT 2 cut(s) 415, 553
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 1 cut(s) 20
Ama87I CYCGRG 2 cut(s) 248, 316
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 3 cut(s) 204, 366, 550
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 229, 433
AvaI CYCGRG 2 cut(s) 248, 316
AvaII GGWCC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 371
BbvI GCAGC 3 cut(s) 216, 378, 562
BccI CCATC 1 cut(s) 418
BceAI ACGGC 3 cut(s) 42, 88, 449
BciT130I CCWGG 1 cut(s) 10
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 5 cut(s) 77, 101, 205, 367, 551
BlsI GCNGC 5 cut(s) 78, 102, 206, 368, 552
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 2 cut(s) 248, 316
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 246, 247
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 104, 240, 297
BsaWI WCCGGW 2 cut(s) 196, 228
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 376, 406, 509
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 3 cut(s) 104, 240, 297
BseGI GGATG 2 cut(s) 237, 496
BseLI CCNNNNNNNGG 4 cut(s) 268, 376, 406, 509
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 3 cut(s) 368, 385, 488
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 3 cut(s) 225, 425, 471
BseXI GCAGC 3 cut(s) 216, 378, 562
BsgI GTGCAG 1 cut(s) 7
BshFI GGCC 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 248, 316
BsiSI CCGG 4 cut(s) 180, 197, 229, 363
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 4 cut(s) 268, 376, 406, 509
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 1 cut(s) 383
BsoBI CYCGRG 2 cut(s) 248, 316
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 2 cut(s) 12, 484
BspACI CCGC 6 cut(s) 77, 101, 191, 207, 397, 429
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 3 cut(s) 367, 384, 487
BspLI GGNNCC 2 cut(s) 246, 247
BspPI GGATC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 104, 240, 297
BssMI GATC 2 cut(s) 12, 484
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 421, 504, 580
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 4 cut(s) 354, 371, 474, 554
BstDSI CCRYGG 2 cut(s) 104, 297
BstF5I GGATG 2 cut(s) 237, 496
BstKTI GATC 2 cut(s) 15, 487
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 12, 484
BstMWI GCNNNNNNNGC 4 cut(s) 47, 106, 372, 470
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 216, 378, 562
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 2 cut(s) 104, 297
BtsCI GGATG 2 cut(s) 237, 496
Cac8I GCNNGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 245
CviAII CATG 2 cut(s) 389, 539
CviJI RGCY 7 cut(s) 65, 109, 168, 369, 383, 415, 553
CviKI_1 RGCY 7 cut(s) 65, 109, 168, 369, 383, 415, 553
DdeI CTNAG 4 cut(s) 354, 371, 474, 554
DpnI GATC 2 cut(s) 14, 486
DpnII GATC 2 cut(s) 12, 484
EciI GGCGGA 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 245
Eco88I CYCGRG 2 cut(s) 248, 316
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 8
FaeI CATG 2 cut(s) 392, 542
FaiI YATR 6 cut(s) 124, 290, 329, 390, 528, 540
FaqI GGGAC 1 cut(s) 258
FatI CATG 2 cut(s) 388, 538
FauI CCCGC 1 cut(s) 404
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 5 cut(s) 77, 101, 205, 367, 551
FokI GGATG 2 cut(s) 244, 503
Fsp4HI GCNGC 5 cut(s) 77, 101, 205, 367, 551
GluI GCNGC 5 cut(s) 77, 101, 205, 367, 551
HaeIII GGCC 1 cut(s) 383
HapII CCGG 4 cut(s) 180, 197, 229, 363
Hin1II CATG 2 cut(s) 392, 542
HinfI GANTC 2 cut(s) 285, 321
HpaII CCGG 4 cut(s) 180, 197, 229, 363
HphI GGTGA 2 cut(s) 229, 433
Hpy188I TCNGA 2 cut(s) 477, 576
Hpy188III TCNNGA 2 cut(s) 318, 488
Hpy99I CGWCG 2 cut(s) 46, 100
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 3 cut(s) 421, 504, 580
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 3 cut(s) 24, 164, 550
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 106, 372, 470
HpyF3I CTNAG 4 cut(s) 354, 371, 474, 554
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 2 cut(s) 392, 542
KflI GGGWCCC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 2 cut(s) 12, 484
LmnI GCTCC 3 cut(s) 212, 301, 412
Lsp1109I GCAGC 3 cut(s) 216, 378, 562
LweI GCATC 1 cut(s) 222
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 14, 486
MboI GATC 2 cut(s) 12, 484
MboII GAAGA 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 306
MnlI CCTC 8 cut(s) 68, 203, 235, 363, 373, 380, 403, 449
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 4 cut(s) 180, 197, 229, 363
MspR9I CCNGG 1 cut(s) 10
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 47, 106, 372, 470
NdeII GATC 2 cut(s) 12, 484
NlaIII CATG 2 cut(s) 392, 542
NlaIV GGNNCC 2 cut(s) 246, 247
NspI RCATGY 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PfeI GAWTC 2 cut(s) 285, 321
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 5 cut(s) 78, 102, 206, 368, 552
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 246, 247
PspPI GGNCC 1 cut(s) 245
PspPPI RGGWCCY 1 cut(s) 245
SatI GCNGC 5 cut(s) 77, 101, 205, 367, 551
Sau3AI GATC 2 cut(s) 12, 484
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 1 cut(s) 10
SduI GDGCHC 1 cut(s) 306
SetI ASST 7 cut(s) 47, 85, 117, 339, 344, 417, 555
SfaNI GCATC 1 cut(s) 222
SinI GGWCC 1 cut(s) 245
SsiI CCGC 6 cut(s) 77, 101, 191, 207, 397, 429
StyD4I CCNGG 1 cut(s) 8
TaaI ACNGT 3 cut(s) 421, 504, 580
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 111
TauI GCSGC 2 cut(s) 79, 103
TfiI GAWTC 2 cut(s) 285, 321
TseI GCWGC 3 cut(s) 204, 366, 550
TspDTI ATGAA 2 cut(s) 208, 277
TspGWI ACGGA 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 245
XceI RCATGY 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.