Prupe.8G265000_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0017131
Physical Location & Seq
Reverse (-)
22211634 .. 22212206
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G265000.1

Sequence Viewer

Length: 573 bp
ATGCTGGCCAGGATCATATTTTGCAGTGCAGTCCTGTTGGCAACCCTGGCAGTGATTCTGCTGGCTTTGTTTTCACCACTACCTAAAAGAAAGCCATCACCGCCATGGCTGGACCTCTCCTTGTACATCCAGCAACCACAAAGTCCCAATATTATTGATGCAACAAGCTCCAATAATAATAATGATCATGTGGCACAATCTTCTCAATCAGACGGCGGAGGAGCATTCATCTTCCACCGCATGCTCACAGAGGGACCCGAGAACACTTCTCTGGTGGTGGGGAAAGCGCAAGGGTTCATTATCCCAGTAGAGCAGTTTGCAAACTCGGGTTTCAACATAATCTATCTCACCTTTCACACGCCTCAGTATTCGGGTAGCCTAAGCGTTGAGGCCAAGCATGTCTCCCCGAAAGACACCGAAGAGCTTACTGTGATGGGTGGAACGGGGTCGTTTGCTTTTGCAAGAGGACTTGCGGTTTTTGCTCAGAGTATCCATGGTGATGCCACTTATCATGTAAAGTTGCAGCTTAGATTTCCTAAGAATCAGTCTCGGACGACCACAATTCTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

20.57

Weight (kDa)

7.93

Isoelectric Point (pI)

57.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42655 AT5G42655
fragaria_vesca FvH4_2g26081
malus_domestica MD03G1292000.v1.1 MD11G1306400.v1.1
prunus_persica Prupe.8G265000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294451
rosa_laevigata RLG00000011868
rosa_roxburghii Rroxscaffold_7G00173320
rosa_samantha Rh6AG357300 Rh6BG364200 Rh6CG371300 Rh6DG358100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 101, 216, 238, 473
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 1 cut(s) 334
AjnI CCWGG 2 cut(s) 8, 45
AluBI AGCT 3 cut(s) 168, 424, 526
AluI AGCT 3 cut(s) 168, 424, 526
Alw26I GTCTC 2 cut(s) 406, 552
AlwI GGATC 1 cut(s) 20
Ama87I CYCGRG 2 cut(s) 257, 325
AoxI GGCC 2 cut(s) 6, 390
ApeKI GCWGC 1 cut(s) 523
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 112, 254
AsuHPI GGTGA 4 cut(s) 66, 90, 340, 509
AvaI CYCGRG 2 cut(s) 257, 325
AvaII GGWCC 2 cut(s) 112, 254
BalI TGGCCA 1 cut(s) 8
BbvI GCAGC 1 cut(s) 535
BccI CCATC 2 cut(s) 103, 427
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 2 cut(s) 10, 47
BciVI GTATCC 1 cut(s) 500
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 406, 552
BfuI GTATCC 1 cut(s) 500
BisI GCNGC 1 cut(s) 524
BlsI GCNGC 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 10, 47
Bme18I GGWCC 2 cut(s) 112, 254
BmeT110I CYCGRG 2 cut(s) 257, 325
BmgT120I GGNCC 2 cut(s) 112, 254
BmiI GGNNCC 2 cut(s) 255, 256
BmrFI CCNGG 2 cut(s) 10, 47
BmrI ACTGGG 1 cut(s) 299
BmsI GCATC 2 cut(s) 148, 490
BmuI ACTGGG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 45, 104, 493
Bse1I ACTGG 1 cut(s) 305
BseBI CCWGG 2 cut(s) 10, 47
BseDI CCNNGG 3 cut(s) 45, 104, 493
BseGI GGATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 377, 497
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 2 cut(s) 8, 392
BsiHKCI CYCGRG 2 cut(s) 257, 325
BslFI GGGAC 2 cut(s) 129, 267
BsmAI GTCTC 2 cut(s) 406, 552
BsmFI GGGAC 2 cut(s) 129, 267
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 2 cut(s) 8, 392
BsoBI CYCGRG 2 cut(s) 257, 325
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 12, 184
Bsp19I CCATGG 2 cut(s) 104, 493
BspACI CCGC 4 cut(s) 101, 216, 238, 473
BspANI GGCC 2 cut(s) 8, 392
BspCNI CTCAG 2 cut(s) 376, 496
BspLI GGNNCC 2 cut(s) 255, 256
BspPI GGATC 1 cut(s) 20
BspQI GCTCTTC 1 cut(s) 414
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 45, 104, 493
BssMI GATC 2 cut(s) 12, 184
BssT1I CCWWGG 2 cut(s) 104, 493
Bst2UI CCWGG 2 cut(s) 10, 47
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 414
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 6, 63, 242
BstDEI CTNAG 5 cut(s) 363, 380, 483, 527, 537
BstDSI CCRYGG 2 cut(s) 104, 493
BstF5I GGATG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 15, 187
BstMAI GTCTC 2 cut(s) 406, 552
BstMBI GATC 2 cut(s) 12, 184
BstMWI GCNNNNNNNGC 3 cut(s) 47, 100, 479
BstNI CCWGG 2 cut(s) 10, 47
BstNSI RCATGY 2 cut(s) 244, 401
BstSCI CCNGG 2 cut(s) 8, 45
BstV1I GCAGC 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 565
BsuI GTATCC 1 cut(s) 500
BsuRI GGCC 2 cut(s) 8, 392
BtgI CCRYGG 2 cut(s) 104, 493
BtsCI GGATG 1 cut(s) 126
BtsI GCAGTG 2 cut(s) 31, 57
BtsIMutI CAGTG 2 cut(s) 31, 57
Cac8I GCNNGC 3 cut(s) 6, 63, 242
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 112, 254
Csp6I GTAC 1 cut(s) 124
CviAII CATG 6 cut(s) 105, 188, 241, 398, 494, 512
CviJI RGCY 9 cut(s) 8, 65, 94, 109, 168, 378, 392, 424, 526
CviKI_1 RGCY 9 cut(s) 8, 65, 94, 109, 168, 378, 392, 424, 526
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 5 cut(s) 363, 380, 483, 527, 537
DpnI GATC 2 cut(s) 14, 186
DpnII GATC 2 cut(s) 12, 184
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 104, 493
Eco47I GGWCC 2 cut(s) 112, 254
Eco88I CYCGRG 2 cut(s) 257, 325
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 8, 45
EcoT14I CCWWGG 2 cut(s) 104, 493
ErhI CCWWGG 2 cut(s) 104, 493
FaeI CATG 6 cut(s) 108, 191, 244, 401, 497, 515
FaiI YATR 8 cut(s) 17, 106, 189, 242, 338, 399, 495, 513
FaqI GGGAC 2 cut(s) 129, 267
FatI CATG 6 cut(s) 104, 187, 240, 397, 493, 511
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 524
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 524
GlaI GCGC 1 cut(s) 288
GluI GCNGC 1 cut(s) 524
HaeIII GGCC 2 cut(s) 8, 392
HhaI GCGC 1 cut(s) 289
Hin1II CATG 6 cut(s) 108, 191, 244, 401, 497, 515
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HinfI GANTC 2 cut(s) 55, 541
HphI GGTGA 4 cut(s) 66, 90, 340, 509
Hpy188I TCNGA 3 cut(s) 211, 486, 552
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4V TGCA 6 cut(s) 24, 29, 161, 320, 461, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 100, 479
HpyF3I CTNAG 5 cut(s) 363, 380, 483, 527, 537
Hsp92II CATG 6 cut(s) 108, 191, 244, 401, 497, 515
HspAI GCGC 1 cut(s) 287
KflI GGGWCCC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 12, 184
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 173, 221
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 2 cut(s) 148, 490
MalI GATC 2 cut(s) 14, 186
MboI GATC 2 cut(s) 12, 184
MboII GAAGA 3 cut(s) 192, 223, 431
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 8
MnlI CCTC 6 cut(s) 125, 212, 244, 372, 382, 458
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MslI CAYNNNNRTG 2 cut(s) 103, 498
Msp20I TGGCCA 1 cut(s) 8
MspR9I CCNGG 2 cut(s) 10, 47
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 2 cut(s) 10, 47
MwoI GCNNNNNNNGC 3 cut(s) 47, 100, 479
NcoI CCATGG 2 cut(s) 104, 493
NdeII GATC 2 cut(s) 12, 184
NlaIII CATG 6 cut(s) 108, 191, 244, 401, 497, 515
NlaIV GGNNCC 2 cut(s) 255, 256
NspI RCATGY 2 cut(s) 244, 401
PaeI GCATGC 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 55, 541
PkrI GCNGC 1 cut(s) 525
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 8, 45
PspGI CCWGG 2 cut(s) 8, 45
PspN4I GGNNCC 2 cut(s) 255, 256
PspPI GGNCC 2 cut(s) 112, 254
PspPPI RGGWCCY 1 cut(s) 254
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 2 cut(s) 103, 498
SapI GCTCTTC 1 cut(s) 414
SatI GCNGC 1 cut(s) 524
Sau3AI GATC 2 cut(s) 12, 184
Sau96I GGNCC 2 cut(s) 112, 254
ScrFI CCNGG 2 cut(s) 10, 47
SetI ASST 6 cut(s) 85, 117, 170, 353, 426, 528
SfaNI GCATC 2 cut(s) 148, 490
SinI GGWCC 2 cut(s) 112, 254
SmiMI CAYNNNNRTG 2 cut(s) 103, 498
SphI GCATGC 1 cut(s) 244
Sse9I AATT 1 cut(s) 561
SsiI CCGC 4 cut(s) 101, 216, 238, 473
SspI AATATT 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 8, 45
StyI CCWWGG 2 cut(s) 104, 493
TaaI ACNGT 1 cut(s) 430
TasI AATT 1 cut(s) 561
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 2 cut(s) 55, 541
TscAI CASTG 2 cut(s) 31, 57
TseI GCWGC 1 cut(s) 523
TspDTI ATGAA 2 cut(s) 217, 286
TspRI CASTG 2 cut(s) 31, 57
VpaK11BI GGWCC 2 cut(s) 112, 254
XceI RCATGY 2 cut(s) 244, 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.