FvH4_2g26511

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010529
Physical Location & Seq
Forward (+)
21276383 .. 21278480
2098 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26511.t2

Sequence Viewer

Length: 1290 bp
ATGACGTCAGCGATCGGATGGTACGGGCCGCTAATCGACCTGTCGGAGGCGGCGCTCCACATCGGCGACTTCGTACAGCTGGTCGTCTTTGTCCACCGAATCACTCCTCTTCAGTATAAATCATCGAACGGCGGAGAGGTGATCCGGACGGACATCCAGGTCGGCGACGAAACGCGGCCGTTTTTCTCCGTTTCCCTGTGGCAAAAGCAAATGGGAACCACGGCAGTCTACGGCGACGTCGTTTTGTTACAGAATATGCAGATAGTAAAATATCGTGATGTTGTTGAAGCTAAAACCGTCACACAGTCTTCCTTACAATGTCTACTTCATCCTTATGAATCCATTTTAACCAAAGGCGTTGATGAGTTGATAGAGGGGTGTCGAGTTGGGGTAGCTGTGAAGGAGAAGCTAAGAAAGGTAGTGGAGTGGGTGAAGAAGAGCAACACTCGTCCGTTACGCGGCTCTCAGCTGCGGCAGAGGCAATTCCCGAGGAACTGGAAGCTGCTGGAAGAGAGTCAAAAGCCTGAGGATTGTTGTTCAATTTTGGAGTTATCACAGCTAACTAATTCTTGCAAGGCAGTGTTTGAAGCATCTGTTGCCGAAATTTTTATGCCACTAGCAGAATATGAGTCTTTGTGTGACAAGGAAGACATGTTTGCAAGTAGGAGAGTGTGTAAAACAGGAGATCCTGGTTTGGTGAAGCATCTTATTTGTACAGGCTGCCGGATATGTGGTTTGCCCTTGGAGGCGAAAAGTGGAAACACGTCTAAGCAAAATGCAGCTGCTCTTTACTGTTCGGAGAGCTCGAACCGCCATCATGTTATAAGCCTCATATACAGGCCTTTCATGTTATATGTATGGGATGATTCAGATTATGCACCGCTCCTAGTCAAAAACAAAGCCGCAGAACTTCTGTTTGGAAACATAAAGGCTGAAAGAGTCTACTCCTGCTATAAGGAAAAACCAAATGACAGAGAAGTTAATTTCAGAGACCCTGACACAGAAACTGGTACAAGTGCAAAACCAACCTCACAACCTAAAGCTGCTGAAGGAGTTCTGGTTTCTTGCTCATCAGCTGTACACAAAAAGAGCTTAGAATGGAAGGGAAGACTTGATTTCCAGACGAACATGGACTTCTATAGGATTTGGTTAATTCTTCTGAAAACTATGCTGCAGCAAAGAACGAACAGCCCTTTGAAATTCGTAGTCAATATAAATGCCGGTTTGGATAGGGAAAATGGGAGGTTTGAAATGATTTCTGTGCAGATGCCATGCTTAAGCACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

48.51

Weight (kDa)

8.54

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AasI GACNNNNNNGTC 1 cut(s) 158
AatII GACGTC 2 cut(s) 8, 240
AccBSI CCGCTC 1 cut(s) 883
AccI GTMKAC 3 cut(s) 228, 322, 942
AccII CGCG 2 cut(s) 175, 459
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 9 cut(s) 29, 50, 132, 175, 459, 472, 811, 881, 903
AclWI GGATC 2 cut(s) 136, 680
AcoI YGGCCR 1 cut(s) 176
AcsI RAATTY 2 cut(s) 603, 1199
AcuI CTGAAG 2 cut(s) 95, 1068
AcyI GRCGYC 2 cut(s) 5, 237
AdeI CACNNNGTG 1 cut(s) 1287
AfaI GTAC 5 cut(s) 23, 75, 715, 1012, 1080
AfiI CCNNNNNNNGG 2 cut(s) 46, 458
AflII CTTAAG 1 cut(s) 1276
AflIII ACRYGT 2 cut(s) 651, 762
AgsI TTSAA 5 cut(s) 287, 540, 587, 1198, 1250
AjiI CACGTC 1 cut(s) 765
AjnI CCWGG 3 cut(s) 156, 688, 1283
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 1 cut(s) 984
AlwI GGATC 2 cut(s) 136, 680
AlwNI CAGNNNCTG 1 cut(s) 1007
Ama87I CYCGRG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 3 cut(s) 26, 176, 839
ApeKI GCWGC 8 cut(s) 469, 502, 720, 779, 782, 1043, 1171, 1174
ApoI RAATTY 2 cut(s) 603, 1199
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 151, 442, 709
AvaI CYCGRG 1 cut(s) 487
AxyI CCTNAGG 1 cut(s) 525
BanII GRGCYC 1 cut(s) 806
BbsI GAAGAC 3 cut(s) 300, 654, 1114
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 8 cut(s) 456, 489, 707, 769, 791, 1030, 1158, 1186
BccI CCATC 2 cut(s) 12, 822
BceAI ACGGC 4 cut(s) 145, 163, 237, 247
BciT130I CCWGG 3 cut(s) 158, 690, 1285
BcoDI GTCTC 1 cut(s) 984
BfaI CTAG 2 cut(s) 617, 887
BfmI CTRYAG 2 cut(s) 1138, 1172
BfoI RGCGCY 1 cut(s) 56
BfrI CTTAAG 1 cut(s) 1276
Bme1390I CCNGG 3 cut(s) 158, 690, 1285
BmeT110I CYCGRG 1 cut(s) 487
BmgBI CACGTC 1 cut(s) 765
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 158, 690, 1285
BmsI GCATC 3 cut(s) 599, 712, 1257
BpiI GAAGAC 3 cut(s) 300, 654, 1114
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BsaHI GRCGYC 2 cut(s) 5, 237
BsaI GGTCTC 1 cut(s) 984
BsaJI CCNNGG 3 cut(s) 219, 488, 741
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 4 cut(s) 658, 688, 1044, 1074
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 458
Bse118I RCCGGY 1 cut(s) 1220
Bse1I ACTGG 2 cut(s) 500, 1012
Bse21I CCTNAGG 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 3 cut(s) 158, 690, 1285
BseDI CCNNGG 3 cut(s) 219, 488, 741
BseGI GGATG 4 cut(s) 23, 153, 328, 868
BseLI CCNNNNNNNGG 2 cut(s) 46, 458
BseMII CTCAG 2 cut(s) 479, 516
BseNI ACTGG 2 cut(s) 500, 1012
BseRI GAGGAG 1 cut(s) 96
BseX3I CGGCCG 1 cut(s) 176
BseXI GCAGC 8 cut(s) 456, 489, 707, 769, 791, 1030, 1158, 1186
BsgI GTGCAG 1 cut(s) 1283
Bsh1236I CGCG 2 cut(s) 175, 459
Bsh1285I CGRYCG 2 cut(s) 15, 179
BshFI GGCC 3 cut(s) 28, 178, 841
BsiEI CGRYCG 2 cut(s) 15, 179
BsiHKAI GWGCWC 1 cut(s) 806
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 3 cut(s) 145, 724, 1221
BslI CCNNNNNNNGG 2 cut(s) 46, 458
BsmAI GTCTC 1 cut(s) 984
BsnI GGCC 3 cut(s) 28, 178, 841
Bso31I GGTCTC 1 cut(s) 984
BsoBI CYCGRG 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 806
Bsp13I TCCGGA 1 cut(s) 144
Bsp1407I TGTACA 2 cut(s) 713, 1078
Bsp143I GATC 3 cut(s) 12, 141, 685
BspACI CCGC 9 cut(s) 29, 50, 132, 175, 459, 472, 811, 881, 903
BspANI GGCC 3 cut(s) 28, 178, 841
BspCNI CTCAG 2 cut(s) 478, 517
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 2 cut(s) 175, 459
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 1176
BspPI GGATC 2 cut(s) 136, 680
BspQI GCTCTTC 1 cut(s) 431
BspTI CTTAAG 1 cut(s) 1276
BspTNI GGTCTC 1 cut(s) 984
BsrBI CCGCTC 1 cut(s) 883
BsrFI RCCGGY 1 cut(s) 1220
BsrGI TGTACA 2 cut(s) 713, 1078
BsrI ACTGG 2 cut(s) 500, 1012
BssAI RCCGGY 1 cut(s) 1220
BssECI CCNNGG 3 cut(s) 219, 488, 741
BssMI GATC 3 cut(s) 12, 141, 685
BssNI GRCGYC 2 cut(s) 5, 237
BssT1I CCWWGG 1 cut(s) 741
Bst2UI CCWGG 3 cut(s) 158, 690, 1285
Bst4CI ACNGT 3 cut(s) 298, 306, 794
Bst6I CTCTTC 3 cut(s) 114, 431, 504
BstACI GRCGYC 2 cut(s) 5, 237
BstAFI CTTAAG 1 cut(s) 1276
BstAPI GCANNNNNTGC 1 cut(s) 596
BstAUI TGTACA 2 cut(s) 713, 1078
BstDEI CTNAG 5 cut(s) 410, 465, 525, 768, 1093
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 4 cut(s) 23, 153, 328, 868
BstFNI CGCG 2 cut(s) 175, 459
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 3 cut(s) 15, 144, 688
BstMAI GTCTC 1 cut(s) 984
BstMBI GATC 3 cut(s) 12, 141, 685
BstMCI CGRYCG 2 cut(s) 15, 179
BstMWI GCNNNNNNNGC 3 cut(s) 478, 596, 810
BstNI CCWGG 3 cut(s) 158, 690, 1285
BstNSI RCATGY 1 cut(s) 655
BstSCI CCNGG 3 cut(s) 156, 688, 1283
BstSFI CTRYAG 2 cut(s) 1138, 1172
BstUI CGCG 2 cut(s) 175, 459
BstV1I GCAGC 8 cut(s) 456, 489, 707, 769, 791, 1030, 1158, 1186
BstV2I GAAGAC 3 cut(s) 300, 654, 1114
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BstZI CGGCCG 1 cut(s) 176
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 3 cut(s) 28, 178, 841
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 765
BtsCI GGATG 4 cut(s) 23, 153, 328, 868
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 1 cut(s) 585
CaiI CAGNNNCTG 1 cut(s) 1007
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 1220
Cfr13I GGNCC 1 cut(s) 26
CsiI ACCWGGT 1 cut(s) 1283
Csp6I GTAC 5 cut(s) 22, 74, 714, 1011, 1079
CviAII CATG 5 cut(s) 652, 818, 847, 1129, 1272
CviQI GTAC 5 cut(s) 22, 74, 714, 1011, 1079
DdeI CTNAG 5 cut(s) 410, 465, 525, 768, 1093
DpnI GATC 3 cut(s) 14, 143, 687
DpnII GATC 3 cut(s) 12, 141, 685
DraIII CACNNNGTG 1 cut(s) 1287
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 176
EagI CGGCCG 1 cut(s) 176
Eam1104I CTCTTC 3 cut(s) 114, 431, 504
EarI CTCTTC 3 cut(s) 114, 431, 504
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 804
EclXI CGGCCG 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 741
Eco147I AGGCCT 1 cut(s) 841
Eco24I GRGCYC 1 cut(s) 806
Eco31I GGTCTC 1 cut(s) 984
Eco52I CGGCCG 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 804
Eco57I CTGAAG 2 cut(s) 95, 1068
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 804
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 156, 688, 1283
EcoT14I CCWWGG 1 cut(s) 741
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 741
FaeI CATG 5 cut(s) 655, 821, 850, 1132, 1275
FatI CATG 5 cut(s) 651, 817, 846, 1128, 1271
FblI GTMKAC 3 cut(s) 228, 322, 942
FokI GGATG 4 cut(s) 30, 140, 315, 875
FriOI GRGCYC 1 cut(s) 806
FspBI CTAG 2 cut(s) 617, 887
GlaI GCGC 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 3 cut(s) 28, 178, 841
HapII CCGG 3 cut(s) 145, 724, 1221
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 2 cut(s) 5, 237
Hin1II CATG 5 cut(s) 655, 821, 850, 1132, 1275
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HinfI GANTC 6 cut(s) 99, 338, 514, 629, 866, 939
HpaII CCGG 3 cut(s) 145, 724, 1221
HphI GGTGA 3 cut(s) 151, 442, 709
Hpy166II GTNNAC 5 cut(s) 94, 229, 323, 943, 1081
Hpy188I TCNGA 6 cut(s) 17, 46, 799, 871, 989, 1161
Hpy188III TCNNGA 4 cut(s) 145, 275, 487, 1120
Hpy8I GTNNAC 5 cut(s) 94, 229, 323, 943, 1081
Hpy99I CGWCG 3 cut(s) 170, 239, 242
HpyAV CCTTC 3 cut(s) 394, 1043, 1096
HpyCH4III ACNGT 3 cut(s) 298, 306, 794
HpyCH4IV ACGT 3 cut(s) 5, 237, 764
HpyCH4V TGCA 8 cut(s) 259, 573, 659, 779, 878, 1019, 1174, 1264
HpyF10VI GCNNNNNNNGC 3 cut(s) 478, 596, 810
HpyF3I CTNAG 5 cut(s) 410, 465, 525, 768, 1093
HpySE526I ACGT 3 cut(s) 5, 237, 764
Hsp92I GRCGYC 2 cut(s) 5, 237
Hsp92II CATG 5 cut(s) 655, 821, 850, 1132, 1275
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 3 cut(s) 12, 141, 685
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 2 cut(s) 60, 888
Lsp1109I GCAGC 8 cut(s) 456, 489, 707, 769, 791, 1030, 1158, 1186
LweI GCATC 3 cut(s) 599, 712, 1257
MabI ACCWGGT 1 cut(s) 1283
MaeI CTAG 2 cut(s) 617, 887
MaeII ACGT 3 cut(s) 5, 237, 764
MaeIII GTNAC 4 cut(s) 246, 298, 453, 638
MalI GATC 3 cut(s) 14, 143, 687
MbiI CCGCTC 1 cut(s) 883
MboI GATC 3 cut(s) 12, 141, 685
MboII GAAGA 8 cut(s) 101, 300, 445, 448, 521, 659, 1119, 1148
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 1 cut(s) 806
MluCI AATT 7 cut(s) 482, 540, 565, 603, 982, 1152, 1199
MlyI GAGTC 3 cut(s) 523, 638, 948
MmeI TCCRAC 1 cut(s) 24
MroI TCCGGA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 1053
MseI TTAA 4 cut(s) 347, 981, 1151, 1277
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 4 cut(s) 79, 469, 782, 1076
MspCI CTTAAG 1 cut(s) 1276
MspI CCGG 3 cut(s) 145, 724, 1221
MspR9I CCNGG 3 cut(s) 158, 690, 1285
MvaI CCWGG 3 cut(s) 158, 690, 1285
MvnI CGCG 2 cut(s) 175, 459
MwoI GCNNNNNNNGC 3 cut(s) 478, 596, 810
NdeII GATC 3 cut(s) 12, 141, 685
NlaIII CATG 5 cut(s) 655, 821, 850, 1132, 1275
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 298, 638
NspI RCATGY 1 cut(s) 655
PceI AGGCCT 1 cut(s) 841
PciI ACATGT 1 cut(s) 651
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 237, 803
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 3 cut(s) 99, 338, 866
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 3 cut(s) 522, 637, 947
PpsI GAGTC 3 cut(s) 522, 637, 947
PscI ACATGT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 824
Psp124BI GAGCTC 1 cut(s) 806
Psp6I CCWGG 3 cut(s) 156, 688, 1283
PspGI CCWGG 3 cut(s) 156, 688, 1283
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 26
PstI CTGCAG 1 cut(s) 1176
PstNI CAGNNNCTG 1 cut(s) 1007
PsuI RGATCY 1 cut(s) 685
PvuI CGATCG 1 cut(s) 15
PvuII CAGCTG 4 cut(s) 79, 469, 782, 1076
RsaI GTAC 5 cut(s) 23, 75, 715, 1012, 1080
RsaNI GTAC 5 cut(s) 22, 74, 714, 1011, 1079
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 806
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 4 cut(s) 347, 981, 1151, 1277
Sau3AI GATC 3 cut(s) 12, 141, 685
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 3 cut(s) 523, 638, 948
ScrFI CCNGG 3 cut(s) 158, 690, 1285
SduI GDGCHC 1 cut(s) 806
SexAI ACCWGGT 1 cut(s) 1283
SfaNI GCATC 3 cut(s) 599, 712, 1257
SfcI CTRYAG 2 cut(s) 1138, 1172
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 1276
SmoI CTYRAG 1 cut(s) 1276
Sse9I AATT 7 cut(s) 482, 540, 565, 603, 982, 1152, 1199
SseBI AGGCCT 1 cut(s) 841
SsiI CCGC 9 cut(s) 29, 50, 132, 175, 459, 472, 811, 881, 903
SspMI CTAG 2 cut(s) 617, 887
SstI GAGCTC 1 cut(s) 806
StuI AGGCCT 1 cut(s) 841
StyD4I CCNGG 3 cut(s) 156, 688, 1283
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 3 cut(s) 298, 306, 794
TaiI ACGT 3 cut(s) 8, 240, 767
TaqI TCGA 4 cut(s) 36, 125, 382, 806
TasI AATT 7 cut(s) 482, 540, 565, 603, 982, 1152, 1199
TatI WGTACW 2 cut(s) 713, 1078
TauI GCSGC 6 cut(s) 31, 53, 178, 462, 475, 905
TfiI GAWTC 3 cut(s) 99, 338, 866
Tru1I TTAA 4 cut(s) 347, 981, 1151, 1277
Tru9I TTAA 4 cut(s) 347, 981, 1151, 1277
TscAI CASTG 1 cut(s) 585
TseFI GTSAC 2 cut(s) 298, 638
TseI GCWGC 8 cut(s) 469, 502, 720, 779, 782, 1043, 1171, 1174
Tsp45I GTSAC 2 cut(s) 298, 638
TspDTI ATGAA 3 cut(s) 317, 351, 835
TspGWI ACGGA 3 cut(s) 164, 178, 441
TspRI CASTG 1 cut(s) 585
Vha464I CTTAAG 1 cut(s) 1276
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 2 cut(s) 603, 1199
XceI RCATGY 1 cut(s) 655
XmiI GTMKAC 3 cut(s) 228, 322, 942
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 2 cut(s) 617, 887
ZraI GACGTC 2 cut(s) 6, 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.