FvH4_2g26550

Kynurenine--oxoglutarate transaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012279
Physical Location & Seq
Forward (+)
21302285 .. 21305663
3379 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26550.t1

Sequence Viewer

Length: 1365 bp
ATGCGGATTCAATGTACCTGGCCATCCTCTGAAATGCGTTTGGGTTCTTCAACCTTCTTAAAACATTTGAGCTTTATAAGGAATACAAGTTATAGAACAACCACAGTCCGTCCTCGTTGCCCTTTTGTCTCAGCCACCATGTCCACCGTTTCCACAGAAAAGGATGCCCTTTCGGGTCAAACAGAGTCGACCCAGATAACCCAGAAGCCATTGCAGGTGGCGAAGCGATTGGAAAAATTCAAGACGACAATTTTCACGCAAATGAGTAATCTTGCCGTTAAACATGGCGCAATAAACCTTGGGCAAGGGTTTCCCAACTTTGATGGTCCCGAGTTTGTAAAGGAAGCTGCAATTCAGGCCGTGATAGATGGGAAGAATCAATATGCTCGTGGATATGGGGTTCCTGACTTAAACAATGCCATTGCTGACCGATTCAAGAAAGAAACTGGACTTGTTGTGGACCCTGAGAAAGAGATAACCGTTACCTCTGGGTGCACAGAGGCAATTGCAGCAACCATGTTGGGTTTGATAAATCCTGGTGATGAGGTTATCCTCTTTGCTCCTTTCTATGATTCTTATGAGGCCACTCTTTCCATGGCTGGTGCTAAAGTAAAAGGTATCACACTACGCCCTCCGGATTTTGCTGTCCCCATTGAGGAACTTAAGTCTGCCATCTCAAAGAATACTCGTGCAATTCTTATCAATACTCCGCATAATCCTACTGGAAAGATGTTCACACGAGAGGAACTTGACATCATTGCATCTCTCTGCATTGAAAATGATGTACTGGTGTTCACTGATGAAGTTTATGATAAGTTGGCTTTTGAAATGGATCACATTTCTATGGCTTCTCTTCCTGGAATGTACGAGCGTACAGTAACCCTGAATTCCTTGGGGAAAACATTCTCATTGACTGGCTGGAAGATTGGTTGGGCTATAGCTCCCCCCCACCTGTCATGGGGAGTGCGGCAGGCACACTCTTATCTCACTTTTGCTACCTCCACTCCTATGCAGTGGGCGGCTGCAACAGCTCTCAGAGCCCCAGACTCCTACTTTGTGGAGCTGAAAAGAGATTACTTGGCAAAGAAGGAAATTTTGGTGGAGGGATTGAAGGCTGTCGGTTTCAAAGTGTATCCGTCCAGCGGGACCTACTTTGTAGTTGTAGATCACACTCCTTTTGGGTTACCAAATGATGTTGCATTTTGTGAGTATCTGATCAAGGAAGTCGGGGTGGTGGCAATCCCTACCAGTGTGTTTTATTTGAACCCGGGCGATGGAAAGAATCTAGTCAGATTTACTTTCTGCAAGGATGAGGGAACTCTTAGGGCTGCAGTTGAGAGGATGAAGGAGAAGCTCAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0008144 GO:0008483 GO:0010326 GO:0016740 GO:0016769 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050662 GO:0070279 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

455

Amino Acids

50.21

Weight (kDa)

8.07

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 98 - 447 3.6e-68 Aminotransferase class I and II
Cys_Met_Meta_PP PF01053 140 - 268 4.8e-08 Cys/Met metabolism PLP-dependent enzyme
DegT_DnrJ_EryC1 PF01041 144 - 267 2.4e-06 DegT/DnrJ/EryC1/StrS aminotransferase family
Aminotran_5 PF00266 153 - 269 7.9e-07 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77670
fragaria_vesca FvH4_2g26550 FvH4_2g26550 FvH4_2g26550
malus_domestica MD08G1166900.v1.1 MD15G1353000.v1.1
prunus_persica Prupe.1G501400_v2.0.a1
pyrus_communis pycom08g14300 pycom15g31620
rosa_chinensis RchiOBHm_Chr6g0295421
rosa_laevigata RLG00000011805
rosa_multiflora Rmu_sc0003058.1_g000002
rosa_roxburghii Rroxscaffold_7G00172510
rosa_rugosa Rorug06G0250500
rosa_samantha Rh6AG363900 Rh6BG372100 Rh6CG378500 Rh6DG364900
rosa_wichuraiana Rw6G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
AarI CACCTGC 1 cut(s) 205
Acc36I ACCTGC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 188
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 5 cut(s) 4, 710, 967, 1019, 1143
AclWI GGATC 1 cut(s) 840
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 4 cut(s) 236, 886, 1092, 1359
AfaI GTAC 4 cut(s) 16, 786, 866, 874
AfiI CCNNNNNNNGG 4 cut(s) 655, 958, 1142, 1274
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 9 cut(s) 11, 51, 241, 436, 776, 827, 1111, 1126, 1264
AjnI CCWGG 3 cut(s) 17, 535, 856
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
AloI GAACNNNNNNTCC 2 cut(s) 384, 416
AluBI AGCT 6 cut(s) 72, 347, 941, 1031, 1063, 1354
AluI AGCT 6 cut(s) 72, 347, 941, 1031, 1063, 1354
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 133
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 1 cut(s) 840
Ama87I CYCGRG 2 cut(s) 329, 1267
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 3 cut(s) 20, 357, 582
ApaLI GTGCAC 1 cut(s) 493
ApeKI GCWGC 4 cut(s) 347, 509, 1022, 1328
ApoI RAATTY 4 cut(s) 236, 886, 1092, 1359
ArsI GACNNNNNNTTYG 2 cut(s) 1037, 1069
Asp700I GAANNNNTTC 1 cut(s) 902
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 3 cut(s) 326, 460, 1146
AsuC2I CCSGG 2 cut(s) 1268, 1269
AsuHPI GGTGA 1 cut(s) 551
AvaI CYCGRG 2 cut(s) 329, 1267
AvaII GGWCC 3 cut(s) 326, 460, 1146
BaeGI GKGCMC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 22
BanII GRGCYC 1 cut(s) 1042
BauI CACGAG 3 cut(s) 387, 687, 738
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 334, 521, 1009, 1315
BccI CCATC 5 cut(s) 31, 317, 362, 680, 1268
BceAI ACGGC 2 cut(s) 260, 344
BciT130I CCWGG 3 cut(s) 19, 537, 858
BciVI GTATCC 1 cut(s) 1143
BclI TGATCA 1 cut(s) 1215
BcnI CCSGG 2 cut(s) 1268, 1269
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 1286
BfmI CTRYAG 2 cut(s) 936, 1329
BfrI CTTAAG 1 cut(s) 662
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 1 cut(s) 1143
BisI GCNGC 6 cut(s) 348, 510, 968, 1020, 1023, 1329
BlsI GCNGC 6 cut(s) 349, 511, 969, 1021, 1024, 1330
Bme1390I CCNGG 5 cut(s) 19, 537, 858, 1268, 1269
Bme18I GGWCC 3 cut(s) 326, 460, 1146
BmeT110I CYCGRG 2 cut(s) 329, 1267
BmgT120I GGNCC 3 cut(s) 326, 460, 1146
BmiI GGNNCC 4 cut(s) 328, 402, 462, 1147
BmrFI CCNGG 5 cut(s) 19, 537, 858, 1268, 1269
BmsI GCATC 2 cut(s) 154, 770
BpuMI CCSGG 2 cut(s) 1268, 1269
BsaJI CCNNGG 4 cut(s) 298, 594, 891, 1267
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 4 cut(s) 655, 958, 1142, 1274
Bse1I ACTGG 5 cut(s) 451, 727, 792, 919, 1248
Bse3DI GCAATG 3 cut(s) 209, 420, 756
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 3 cut(s) 19, 537, 858
BseDI CCNNGG 4 cut(s) 298, 594, 891, 1267
BseGI GGATG 4 cut(s) 23, 169, 1315, 1347
BseLI CCNNNNNNNGG 4 cut(s) 655, 958, 1142, 1274
BseMI GCAATG 3 cut(s) 209, 420, 756
BseMII CTCAG 3 cut(s) 144, 456, 1048
BseNI ACTGG 5 cut(s) 451, 727, 792, 919, 1248
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 4 cut(s) 334, 521, 1009, 1315
BshFI GGCC 3 cut(s) 22, 359, 584
BsiHKAI GWGCWC 1 cut(s) 497
BsiHKCI CYCGRG 2 cut(s) 329, 1267
BsiSI CCGG 2 cut(s) 635, 1268
BslFI GGGAC 3 cut(s) 312, 632, 1159
BslI CCNNNNNNNGG 4 cut(s) 655, 958, 1142, 1274
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 3 cut(s) 312, 632, 1159
BsnI GGCC 3 cut(s) 22, 359, 584
BsoBI CYCGRG 2 cut(s) 329, 1267
Bsp1286I GDGCHC 2 cut(s) 497, 1042
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 3 cut(s) 832, 1165, 1215
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 5 cut(s) 4, 710, 967, 1019, 1143
BspANI GGCC 3 cut(s) 22, 359, 584
BspCNI CTCAG 3 cut(s) 143, 457, 1047
BspEI TCCGGA 1 cut(s) 634
BspLI GGNNCC 4 cut(s) 328, 402, 462, 1147
BspMAI CTGCAG 1 cut(s) 1333
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 1 cut(s) 840
BspTI CTTAAG 1 cut(s) 662
BsrDI GCAATG 3 cut(s) 209, 420, 756
BsrI ACTGG 5 cut(s) 451, 727, 792, 919, 1248
BssECI CCNNGG 4 cut(s) 298, 594, 891, 1267
BssMI GATC 3 cut(s) 832, 1165, 1215
BssSI CACGAG 3 cut(s) 387, 687, 738
BssT1I CCWWGG 3 cut(s) 298, 594, 891
Bst2BI CACGAG 3 cut(s) 387, 687, 738
Bst2UI CCWGG 3 cut(s) 19, 537, 858
Bst4CI ACNGT 4 cut(s) 106, 148, 481, 877
Bst6I CTCTTC 1 cut(s) 858
BstAFI CTTAAG 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 972
BstDEI CTNAG 4 cut(s) 130, 465, 1034, 1322
BstDSI CCRYGG 1 cut(s) 594
BstEII GGTNACC 1 cut(s) 1182
BstF5I GGATG 4 cut(s) 23, 169, 1315, 1347
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 3 cut(s) 835, 1168, 1218
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 3 cut(s) 832, 1165, 1215
BstMWI GCNNNNNNNGC 4 cut(s) 356, 509, 1028, 1037
BstNI CCWGG 3 cut(s) 19, 537, 858
BstPI GGTNACC 1 cut(s) 1182
BstSCI CCNGG 5 cut(s) 17, 535, 856, 1266, 1267
BstSFI CTRYAG 2 cut(s) 936, 1329
BstSLI GKGCMC 1 cut(s) 497
BstV1I GCAGC 4 cut(s) 334, 521, 1009, 1315
BsuI GTATCC 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 22, 359, 584
BtgI CCRYGG 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 1287
BtsCI GGATG 4 cut(s) 23, 169, 1315, 1347
BtsI GCAGTG 1 cut(s) 1019
BtsIMutI CAGTG 3 cut(s) 795, 1019, 1255
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 972
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 3 cut(s) 326, 460, 1146
Cfr9I CCCGGG 1 cut(s) 1267
Csp6I GTAC 4 cut(s) 15, 785, 865, 873
CviAII CATG 5 cut(s) 139, 284, 517, 595, 957
CviQI GTAC 4 cut(s) 15, 785, 865, 873
DdeI CTNAG 4 cut(s) 130, 465, 1034, 1322
DpnI GATC 3 cut(s) 834, 1167, 1217
DpnII GATC 3 cut(s) 832, 1165, 1215
EaeI YGGCCR 1 cut(s) 20
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Eco130I CCWWGG 3 cut(s) 298, 594, 891
Eco24I GRGCYC 1 cut(s) 1042
Eco47I GGWCC 3 cut(s) 326, 460, 1146
Eco88I CYCGRG 2 cut(s) 329, 1267
Eco91I GGTNACC 1 cut(s) 1182
EcoO109I RGGNCCY 1 cut(s) 1146
EcoO65I GGTNACC 1 cut(s) 1182
EcoRI GAATTC 1 cut(s) 886
EcoRII CCWGG 3 cut(s) 17, 535, 856
EcoT14I CCWWGG 3 cut(s) 298, 594, 891
EcoT38I GRGCYC 1 cut(s) 1042
ErhI CCWWGG 3 cut(s) 298, 594, 891
FaeI CATG 5 cut(s) 142, 287, 520, 598, 960
FaqI GGGAC 3 cut(s) 312, 632, 1159
FatI CATG 5 cut(s) 138, 283, 516, 594, 956
FauI CCCGC 1 cut(s) 1136
FbaI TGATCA 1 cut(s) 1215
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 6 cut(s) 348, 510, 968, 1020, 1023, 1329
FokI GGATG 4 cut(s) 10, 176, 1322, 1354
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 6 cut(s) 348, 510, 968, 1020, 1023, 1329
FspBI CTAG 1 cut(s) 1286
GlaI GCGC 1 cut(s) 289
GluI GCNGC 6 cut(s) 348, 510, 968, 1020, 1023, 1329
HaeIII GGCC 3 cut(s) 22, 359, 584
HapII CCGG 2 cut(s) 635, 1268
HhaI GCGC 1 cut(s) 290
Hin1II CATG 5 cut(s) 142, 287, 520, 598, 960
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 7 cut(s) 7, 185, 376, 432, 572, 1046, 1282
HpaII CCGG 2 cut(s) 635, 1268
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 6 cut(s) 144, 189, 460, 495, 735, 795
Hpy188I TCNGA 4 cut(s) 31, 1037, 1215, 1292
Hpy188III TCNNGA 5 cut(s) 241, 329, 404, 436, 635
Hpy8I GTNNAC 6 cut(s) 144, 189, 460, 495, 735, 795
HpyAV CCTTC 4 cut(s) 64, 1081, 1105, 1339
HpyCH4III ACNGT 4 cut(s) 106, 148, 481, 877
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 509, 1028, 1037
HpyF3I CTNAG 4 cut(s) 130, 465, 1034, 1322
Hsp92II CATG 5 cut(s) 142, 287, 520, 598, 960
HspAI GCGC 1 cut(s) 288
Kpn2I TCCGGA 1 cut(s) 634
Ksp22I TGATCA 1 cut(s) 1215
Kzo9I GATC 3 cut(s) 832, 1165, 1215
LmnI GCTCC 3 cut(s) 565, 946, 1060
Lsp1109I GCAGC 4 cut(s) 334, 521, 1009, 1315
LweI GCATC 2 cut(s) 154, 770
MaeI CTAG 1 cut(s) 1286
MaeIII GTNAC 3 cut(s) 481, 877, 1182
MalI GATC 3 cut(s) 834, 1167, 1217
MboI GATC 3 cut(s) 832, 1165, 1215
MboII GAAGA 4 cut(s) 39, 385, 845, 934
MfeI CAATTG 1 cut(s) 504
MhlI GDGCHC 2 cut(s) 497, 1042
MlsI TGGCCA 1 cut(s) 22
MluCI AATT 8 cut(s) 236, 249, 351, 504, 693, 886, 1092, 1359
MluNI TGGCCA 1 cut(s) 22
MlyI GAGTC 2 cut(s) 194, 1040
Mox20I TGGCCA 1 cut(s) 22
MroI TCCGGA 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 902
MscI TGGCCA 1 cut(s) 22
MseI TTAA 5 cut(s) 59, 279, 410, 663, 1363
MslI CAYNNNNRTG 3 cut(s) 260, 842, 1007
Msp20I TGGCCA 1 cut(s) 22
MspA1I CMGCKG 1 cut(s) 1143
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 2 cut(s) 635, 1268
MspR9I CCNGG 5 cut(s) 19, 537, 858, 1268, 1269
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 3 cut(s) 19, 537, 858
MwoI GCNNNNNNNGC 4 cut(s) 356, 509, 1028, 1037
NciI CCSGG 2 cut(s) 1268, 1269
NcoI CCATGG 1 cut(s) 594
NdeII GATC 3 cut(s) 832, 1165, 1215
NlaIII CATG 5 cut(s) 142, 287, 520, 598, 960
NlaIV GGNNCC 4 cut(s) 328, 402, 462, 1147
PaqCI CACCTGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 902
PfeI GAWTC 5 cut(s) 7, 376, 432, 572, 1282
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 6 cut(s) 349, 511, 969, 1021, 1024, 1330
PleI GAGTC 2 cut(s) 193, 1040
PpsI GAGTC 2 cut(s) 193, 1040
PpuMI RGGWCCY 1 cut(s) 1146
PsiI TTATAA 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 1146
Psp6I CCWGG 3 cut(s) 17, 535, 856
PspEI GGTNACC 1 cut(s) 1182
PspGI CCWGG 3 cut(s) 17, 535, 856
PspN4I GGNNCC 4 cut(s) 328, 402, 462, 1147
PspPI GGNCC 3 cut(s) 326, 460, 1146
PspPPI RGGWCCY 1 cut(s) 1146
PstI CTGCAG 1 cut(s) 1333
RsaI GTAC 4 cut(s) 16, 786, 866, 874
RsaNI GTAC 4 cut(s) 15, 785, 865, 873
RseI CAYNNNNRTG 3 cut(s) 260, 842, 1007
SalI GTCGAC 1 cut(s) 187
SaqAI TTAA 5 cut(s) 59, 279, 410, 663, 1363
SatI GCNGC 6 cut(s) 348, 510, 968, 1020, 1023, 1329
Sau3AI GATC 3 cut(s) 832, 1165, 1215
Sau96I GGNCC 3 cut(s) 326, 460, 1146
SchI GAGTC 2 cut(s) 194, 1040
ScrFI CCNGG 5 cut(s) 19, 537, 858, 1268, 1269
SduI GDGCHC 2 cut(s) 497, 1042
SfaNI GCATC 2 cut(s) 154, 770
SfcI CTRYAG 2 cut(s) 936, 1329
SinI GGWCC 3 cut(s) 326, 460, 1146
SmaI CCCGGG 1 cut(s) 1269
SmiMI CAYNNNNRTG 3 cut(s) 260, 842, 1007
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 8 cut(s) 236, 249, 351, 504, 693, 886, 1092, 1359
SsiI CCGC 5 cut(s) 4, 710, 967, 1019, 1143
SspMI CTAG 1 cut(s) 1286
StyD4I CCNGG 5 cut(s) 17, 535, 856, 1266, 1267
StyI CCWWGG 3 cut(s) 298, 594, 891
TaaI ACNGT 4 cut(s) 106, 148, 481, 877
TaqI TCGA 1 cut(s) 188
TaqII GACCGA 1 cut(s) 444
TasI AATT 8 cut(s) 236, 249, 351, 504, 693, 886, 1092, 1359
TatI WGTACW 1 cut(s) 784
TauI GCSGC 2 cut(s) 970, 1022
TfiI GAWTC 5 cut(s) 7, 376, 432, 572, 1282
Tru1I TTAA 5 cut(s) 59, 279, 410, 663, 1363
Tru9I TTAA 5 cut(s) 59, 279, 410, 663, 1363
TscAI CASTG 3 cut(s) 802, 1019, 1255
TseI GCWGC 4 cut(s) 347, 509, 1022, 1328
TspDTI ATGAA 2 cut(s) 816, 1358
TspGWI ACGGA 2 cut(s) 98, 1125
TspMI CCCGGG 1 cut(s) 1267
TspRI CASTG 3 cut(s) 802, 1019, 1255
Vha464I CTTAAG 1 cut(s) 662
VneI GTGCAC 1 cut(s) 493
VpaK11BI GGWCC 3 cut(s) 326, 460, 1146
XapI RAATTY 4 cut(s) 236, 886, 1092, 1359
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmaI CCCGGG 1 cut(s) 1267
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 902
XspI CTAG 1 cut(s) 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.