Rroxscaffold_7G00172510

Kynurenine--oxoglutarate transaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012279
Physical Location & Seq
Reverse (-)
12742623 .. 12745280
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00172510.1

Sequence Viewer

Length: 1134 bp
ATGTCCACCGTTTCGACAGAAAAGGATGCCCTTTCGGGTCAAACAGAATCGACCCAGAAGCCATTACAGCAAACTCTTGATCTTGGGTCTTCAATAGTGTTCTTGGTTGGCCTTTTTCCAAGGCAAACACCATTCTCCATCATAGCTTCTTCTCTCAATAAACCTTGGGCAAGGGTTTCCCAACTTGATGGTCCAGAGTTTGTAAAGGAAGCTGCAATTCAGGCCGTGGTAGATGGGAAAAATCAATATGCTCGTGGATATGGGGTTCCAGACCTAAACAATGCCATTGCTGAGAGATTCAAGAAAGACACTGGACTGGTTGTGGACCCTGAGAAAGAAATAACTGTTACCTCTGGGTGCACAGAGGCAATTGCAGCAACCATGTTGGGCTTGATAAATCCTGGTGATGAGGTTATCCTCTTTGCTCCTTTTTATGATTCTTACGAAGCGACTCTTTCCATGGCTGGTGCTAAAGTAAAAGGTATCACACTACGCCCTCCAGATTTTGCTGTCCCCATTGATGAACTGAAGTCTGCAATCTCAAAGAATACTCGTGCAATTCTTATAAATACTCCGCATAATCCTACTGGAAAGATGTTCACTCGAGAGGAACTTGATGCTATTGCATCTCTCTGCATTGAAAATGATGTACTGGTGTTCACTGATGAAGTTTATGATAAGTTGGCTTTTGAAATGGATCACATTTCTATGGCTTCTCTTCCTGGAATGTATGAGCGTACAGCACACTCTTATCTCACTTTTGCTACCTCCACTCCTATGCAATGGGCTGCTGCAACAGCTCTCAGAGCCCCAGACTCCTACTTTCTGGAGCTGAAAAGAGATTACTTGGCAAAGAAGGAAATTTTGGTAGAGGGATTGAAGGCTGTCGGTTTTAAAGTGTATCCATCCAGCGGGACCTACTTTGTGGTTGTAGATCACACTTCTTTTGGGTTGCCAAATGATGTTGCATTTTGTGAGTATCTGATCAAGGAAGTCGGAGTGGTGGCAATCCCTACCAGTGTGTTTTATTTAAACCCAGAAGATGGAAAGAATCTAGTCAGATTTACTTTCTGCAAGGATGAGGGAACTCTTAGGGCTGCAGTTGAGAGGATGAAGAAGAAGTTGAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0008144 GO:0008483 GO:0010326 GO:0016740 GO:0016769 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050662 GO:0070279 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

377

Amino Acids

41.42

Weight (kDa)

5.26

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 65 - 236 2.7e-35 Aminotransferase class I and II
Cys_Met_Meta_PP PF01053 103 - 224 1.7e-08 Cys/Met metabolism PLP-dependent enzyme
Aminotran_5 PF00266 108 - 224 1.1e-07 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 108 - 222 5.4e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
Aminotran_1_2 PF00155 253 - 370 5.9e-14 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77670
fragaria_vesca FvH4_2g26550 FvH4_2g26550 FvH4_2g26550
malus_domestica MD08G1166900.v1.1 MD15G1353000.v1.1
prunus_persica Prupe.1G501400_v2.0.a1
pyrus_communis pycom08g14300 pycom15g31620
rosa_chinensis RchiOBHm_Chr6g0295421
rosa_laevigata RLG00000011805
rosa_multiflora Rmu_sc0003058.1_g000002
rosa_roxburghii Rroxscaffold_7G00172510
rosa_rugosa Rorug06G0250500
rosa_samantha Rh6AG363900 Rh6BG372100 Rh6CG378500 Rh6DG364900
rosa_wichuraiana Rw6G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 1043
AciI CCGC 2 cut(s) 575, 912
AclWI GGATC 1 cut(s) 705
AcsI RAATTY 1 cut(s) 861
AcuI CTGAAG 1 cut(s) 548
AfaI GTAC 2 cut(s) 651, 739
AfiI CCNNNNNNNGG 2 cut(s) 911, 1043
AgsI TTSAA 6 cut(s) 93, 301, 641, 692, 880, 1126
AjnI CCWGG 2 cut(s) 400, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
AluBI AGCT 4 cut(s) 146, 212, 800, 832
AluI AGCT 4 cut(s) 146, 212, 800, 832
Alw21I GWGCWC 1 cut(s) 362
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 1 cut(s) 705
Ama87I CYCGRG 1 cut(s) 603
AoxI GGCC 2 cut(s) 109, 222
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 5 cut(s) 212, 374, 788, 791, 1097
ApoI RAATTY 1 cut(s) 861
AspS9I GGNCC 3 cut(s) 191, 325, 915
AsuHPI GGTGA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 603
AvaII GGWCC 3 cut(s) 191, 325, 915
BaeGI GKGCMC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 811
BauI CACGAG 2 cut(s) 252, 552
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 362
BbvI GCAGC 5 cut(s) 199, 386, 775, 778, 1084
BccI CCATC 5 cut(s) 146, 182, 227, 913, 1037
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 2 cut(s) 402, 723
BciVI GTATCC 1 cut(s) 912
BclI TGATCA 1 cut(s) 984
BfaI CTAG 1 cut(s) 1055
BfmI CTRYAG 1 cut(s) 1098
BfuI GTATCC 1 cut(s) 912
BisI GCNGC 5 cut(s) 213, 375, 789, 792, 1098
BlsI GCNGC 5 cut(s) 214, 376, 790, 793, 1099
Bme1390I CCNGG 2 cut(s) 402, 723
Bme18I GGWCC 3 cut(s) 191, 325, 915
BmeT110I CYCGRG 1 cut(s) 603
BmgT120I GGNCC 3 cut(s) 191, 325, 915
BmiI GGNNCC 3 cut(s) 267, 327, 916
BmrFI CCNGG 2 cut(s) 402, 723
BmsI GCATC 3 cut(s) 16, 607, 635
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 483, 848
BsaJI CCNNGG 4 cut(s) 119, 164, 225, 459
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1043
Bse1I ACTGG 5 cut(s) 316, 321, 592, 657, 1017
Bse3DI GCAATG 2 cut(s) 285, 788
BseBI CCWGG 2 cut(s) 402, 723
BseDI CCNNGG 4 cut(s) 119, 164, 225, 459
BseGI GGATG 4 cut(s) 31, 905, 1084, 1116
BseLI CCNNNNNNNGG 2 cut(s) 911, 1043
BseMI GCAATG 2 cut(s) 285, 788
BseMII CTCAG 3 cut(s) 282, 321, 817
BseNI ACTGG 5 cut(s) 316, 321, 592, 657, 1017
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 5 cut(s) 199, 386, 775, 778, 1084
BshFI GGCC 2 cut(s) 111, 224
BsiHKAI GWGCWC 1 cut(s) 362
BsiHKCI CYCGRG 1 cut(s) 603
BslFI GGGAC 2 cut(s) 497, 928
BslI CCNNNNNNNGG 2 cut(s) 911, 1043
BsmFI GGGAC 2 cut(s) 497, 928
BsnI GGCC 2 cut(s) 111, 224
BsoBI CYCGRG 1 cut(s) 603
Bsp1286I GDGCHC 2 cut(s) 362, 811
Bsp143I GATC 4 cut(s) 79, 697, 934, 984
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 2 cut(s) 575, 912
BspANI GGCC 2 cut(s) 111, 224
BspCNI CTCAG 3 cut(s) 283, 322, 816
BspLI GGNNCC 3 cut(s) 267, 327, 916
BspMAI CTGCAG 1 cut(s) 1102
BspPI GGATC 1 cut(s) 705
BsrDI GCAATG 2 cut(s) 285, 788
BsrI ACTGG 5 cut(s) 316, 321, 592, 657, 1017
BssECI CCNNGG 4 cut(s) 119, 164, 225, 459
BssMI GATC 4 cut(s) 79, 697, 934, 984
BssSI CACGAG 2 cut(s) 252, 552
BssT1I CCWWGG 3 cut(s) 119, 164, 459
Bst2BI CACGAG 2 cut(s) 252, 552
Bst2UI CCWGG 2 cut(s) 402, 723
Bst4CI ACNGT 2 cut(s) 10, 346
Bst6I CTCTTC 1 cut(s) 723
BstDEI CTNAG 4 cut(s) 291, 330, 803, 1091
BstDSI CCRYGG 2 cut(s) 225, 459
BstF5I GGATG 4 cut(s) 31, 905, 1084, 1116
BstKTI GATC 4 cut(s) 82, 700, 937, 987
BstMBI GATC 4 cut(s) 79, 697, 934, 984
BstMWI GCNNNNNNNGC 5 cut(s) 67, 221, 374, 797, 806
BstNI CCWGG 2 cut(s) 402, 723
BstSCI CCNGG 2 cut(s) 400, 721
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 362
BstV1I GCAGC 5 cut(s) 199, 386, 775, 778, 1084
BstV2I GAAGAC 1 cut(s) 81
BstXI CCANNNNNNTGG 1 cut(s) 188
BsuI GTATCC 1 cut(s) 912
BsuRI GGCC 2 cut(s) 111, 224
BtgI CCRYGG 2 cut(s) 225, 459
BtsCI GGATG 4 cut(s) 31, 905, 1084, 1116
BtsIMutI CAGTG 3 cut(s) 309, 660, 1024
Cfr13I GGNCC 3 cut(s) 191, 325, 915
Csp6I GTAC 2 cut(s) 650, 738
CviAII CATG 2 cut(s) 382, 460
CviQI GTAC 2 cut(s) 650, 738
DdeI CTNAG 4 cut(s) 291, 330, 803, 1091
DpnI GATC 4 cut(s) 81, 699, 936, 986
DpnII GATC 4 cut(s) 79, 697, 934, 984
DraI TTTAAA 2 cut(s) 895, 1032
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco130I CCWWGG 3 cut(s) 119, 164, 459
Eco24I GRGCYC 1 cut(s) 811
Eco47I GGWCC 3 cut(s) 191, 325, 915
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 915
EcoRII CCWGG 2 cut(s) 400, 721
EcoT14I CCWWGG 3 cut(s) 119, 164, 459
EcoT38I GRGCYC 1 cut(s) 811
ErhI CCWWGG 3 cut(s) 119, 164, 459
FaeI CATG 2 cut(s) 385, 463
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 2 cut(s) 497, 928
FatI CATG 2 cut(s) 381, 459
FauI CCCGC 1 cut(s) 905
FbaI TGATCA 1 cut(s) 984
Fnu4HI GCNGC 5 cut(s) 213, 375, 789, 792, 1098
FokI GGATG 4 cut(s) 38, 892, 1091, 1123
FriOI GRGCYC 1 cut(s) 811
Fsp4HI GCNGC 5 cut(s) 213, 375, 789, 792, 1098
FspBI CTAG 1 cut(s) 1055
GluI GCNGC 5 cut(s) 213, 375, 789, 792, 1098
GsuI CTGGAG 2 cut(s) 483, 848
HaeIII GGCC 2 cut(s) 111, 224
Hin1II CATG 2 cut(s) 385, 463
HinfI GANTC 6 cut(s) 47, 297, 437, 451, 815, 1051
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 5 cut(s) 6, 325, 360, 600, 660
Hpy188I TCNGA 4 cut(s) 806, 984, 998, 1061
Hpy188III TCNNGA 7 cut(s) 77, 194, 269, 301, 500, 605, 827
Hpy8I GTNNAC 5 cut(s) 6, 325, 360, 600, 660
HpyAV CCTTC 2 cut(s) 850, 874
HpyCH4III ACNGT 2 cut(s) 10, 346
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 221, 374, 797, 806
HpyF3I CTNAG 4 cut(s) 291, 330, 803, 1091
Hsp92II CATG 2 cut(s) 385, 463
Ksp22I TGATCA 1 cut(s) 984
Kzo9I GATC 4 cut(s) 79, 697, 934, 984
LmnI GCTCC 2 cut(s) 430, 829
Lsp1109I GCAGC 5 cut(s) 199, 386, 775, 778, 1084
LweI GCATC 3 cut(s) 16, 607, 635
MaeI CTAG 1 cut(s) 1055
MaeIII GTNAC 1 cut(s) 346
MalI GATC 4 cut(s) 81, 699, 936, 986
MboI GATC 4 cut(s) 79, 697, 934, 984
MboII GAAGA 6 cut(s) 81, 141, 710, 1052, 1126, 1129
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 362, 811
MluCI AATT 4 cut(s) 216, 369, 558, 861
MlyI GAGTC 2 cut(s) 445, 809
MmeI TCCRAC 1 cut(s) 976
MseI TTAA 2 cut(s) 894, 1031
MslI CAYNNNNRTG 2 cut(s) 707, 776
MspA1I CMGCKG 1 cut(s) 912
MspR9I CCNGG 2 cut(s) 402, 723
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 402, 723
MwoI GCNNNNNNNGC 5 cut(s) 67, 221, 374, 797, 806
NcoI CCATGG 1 cut(s) 459
NdeII GATC 4 cut(s) 79, 697, 934, 984
NlaIII CATG 2 cut(s) 385, 463
NlaIV GGNNCC 3 cut(s) 267, 327, 916
PaeR7I CTCGAG 1 cut(s) 603
PfeI GAWTC 4 cut(s) 47, 297, 437, 1051
PflMI CCANNNNNTGG 1 cut(s) 1043
PfoI TCCNGGA 1 cut(s) 721
PkrI GCNGC 5 cut(s) 214, 376, 790, 793, 1099
PleI GAGTC 2 cut(s) 445, 809
PpsI GAGTC 2 cut(s) 445, 809
PpuMI RGGWCCY 1 cut(s) 915
PsiI TTATAA 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 915
Psp6I CCWGG 2 cut(s) 400, 721
PspGI CCWGG 2 cut(s) 400, 721
PspN4I GGNNCC 3 cut(s) 267, 327, 916
PspPI GGNCC 3 cut(s) 191, 325, 915
PspPPI RGGWCCY 1 cut(s) 915
PstI CTGCAG 1 cut(s) 1102
RsaI GTAC 2 cut(s) 651, 739
RsaNI GTAC 2 cut(s) 650, 738
RseI CAYNNNNRTG 2 cut(s) 707, 776
SaqAI TTAA 2 cut(s) 894, 1031
SatI GCNGC 5 cut(s) 213, 375, 789, 792, 1098
Sau3AI GATC 4 cut(s) 79, 697, 934, 984
Sau96I GGNCC 3 cut(s) 191, 325, 915
SchI GAGTC 2 cut(s) 445, 809
ScrFI CCNGG 2 cut(s) 402, 723
SduI GDGCHC 2 cut(s) 362, 811
SfaNI GCATC 3 cut(s) 16, 607, 635
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 603
SinI GGWCC 3 cut(s) 191, 325, 915
SlaI CTCGAG 1 cut(s) 603
SmiMI CAYNNNNRTG 2 cut(s) 707, 776
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
Sse9I AATT 4 cut(s) 216, 369, 558, 861
SsiI CCGC 2 cut(s) 575, 912
SspMI CTAG 1 cut(s) 1055
StyD4I CCNGG 2 cut(s) 400, 721
StyI CCWWGG 3 cut(s) 119, 164, 459
TaaI ACNGT 2 cut(s) 10, 346
TaqI TCGA 3 cut(s) 14, 50, 604
TasI AATT 4 cut(s) 216, 369, 558, 861
TatI WGTACW 1 cut(s) 649
TfiI GAWTC 4 cut(s) 47, 297, 437, 1051
Tru1I TTAA 2 cut(s) 894, 1031
Tru9I TTAA 2 cut(s) 894, 1031
TscAI CASTG 3 cut(s) 316, 667, 1024
TseI GCWGC 5 cut(s) 212, 374, 788, 791, 1097
TspDTI ATGAA 3 cut(s) 537, 681, 1127
TspRI CASTG 3 cut(s) 316, 667, 1024
Van91I CCANNNNNTGG 1 cut(s) 1043
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 3 cut(s) 191, 325, 915
XapI RAATTY 1 cut(s) 861
XhoI CTCGAG 1 cut(s) 603
XspI CTAG 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.