Rh6DG364900

Kynurenine--oxoglutarate transaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012279
Physical Location & Seq
Forward (+)
56194415 .. 56197712
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG364900.1

Sequence Viewer

Length: 1359 bp
ATGCGGATTCAATGTACCTGGCCGTCCCATGAAATGCGCTTGTGTTCTTCAACCCTCTTCACCCTTTCCAAAAATTCGCACAAAGACTTGAGCTTTATTAGGACGAGGACAACCACGGTCTGCCCTTCTGTCTCAGCCACAATGTCCACCGTTTCGACAGAAAAGGATGCCCTTTCGGGTCAAACAGAATCGACCCAGAAGCCATTACAGGTGGCAAAGCGGTTGGAAAAATTCAAGACGACAATCTTCACGCAAATGAGTAATCTTGCTGTTAAACATGGAGCAATAAACCTTGGGCAAGGGTTTCCCAACTTTGATGGTCCGGAGTTTGTAAAGGAAGCTGCAATTCAGGCCGTGATAGATGGGAAAAATCAATATGCTCGTGGATATGGGGTTCCAGACCTAAACAATGCCATTGCTGAGAGATTCAAGAAAGACACTGGACTGGTTGTGGACCCTGAGAAAGAAATAACTGTTACCTCTGGGTGCACAGAGGCAATTGCAGCAACCATGTTGGGCTTGATAAATCCTGGTGATGAGGTTATCCTCTTTGCTCCTTTTTATGATTCTTACGAAGCGACTCTTTCCATGGCTGGTGCTAAAGTAAAAGGTATCACACTACGCCCTCCAGATTTTGCTGTCCCCGTTGATGAACTGAAGTCTGCAATCTCAAAGAATACTCGTGCAATTCTTATAAATACTCCGCATAATCCTACTGGAAAGATGTTCACTCGAGAGGAACTTGACGCTATTGCATCTCTCTGCATTGAAAATGATGTACTGGTGTTCACTGATGAAGTTTATGATAAGTTGGCTTTTGAAATGGATCACATTTCTATGGCTTCTCTTCCTGGAATGTACGAGCGTACAGTAACCCTGAATTCCTTGGGGAAAACATTCTCGTTAACTGGGTGGAAGATCGGTTGGGCTATAGCTCCCCCCCACCTGTCATGGGGAGTGCGGCAGGCACACTCTTATCTCACTTTTGCTACCTCCACTCCTATGCAATGGGCTGCTGCAACAGCTCTCAGAGCCCCAGACTCCTACTTTCTGGAGCTGAAAAGAGATTACTTGGCAAAGAAGGAAATTTTGGTAGAGGGATTGAAGGCTGTCGGTTTTAAAGTGTATCCATCCAGCGGGACCTACTTTGTGGTTGTAGATCACACTTCTTTTGGGTTGCCAAATGATGTTGCATTTTGTGAGTATCTGATCAAGGAAGTCGGAGTGGTGGCAATCCCTACCAGTGTGTTTTATTTAAACCCAGAAGATGGAAAGAATCTAGTCAGATTTACTTTCTGCAAGGATGAGGGAACTCTTAGGGCTGCAGTTGAGAGGATGAAGAAGAAGTTGAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0008144 GO:0008483 GO:0010326 GO:0016740 GO:0016769 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050662 GO:0070279 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

452

Amino Acids

49.88

Weight (kDa)

7.58

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 96 - 445 3.1e-68 Aminotransferase class I and II
Cys_Met_Meta_PP PF01053 150 - 267 2.8e-08 Cys/Met metabolism PLP-dependent enzyme
Aminotran_5 PF00266 151 - 267 1.8e-07 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 151 - 265 3e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77670
fragaria_vesca FvH4_2g26550 FvH4_2g26550 FvH4_2g26550
malus_domestica MD08G1166900.v1.1 MD15G1353000.v1.1
prunus_persica Prupe.1G501400_v2.0.a1
pyrus_communis pycom08g14300 pycom15g31620
rosa_chinensis RchiOBHm_Chr6g0295421
rosa_laevigata RLG00000011805
rosa_multiflora Rmu_sc0003058.1_g000002
rosa_roxburghii Rroxscaffold_7G00172510
rosa_rugosa Rorug06G0250500
rosa_samantha Rh6AG363900 Rh6BG372100 Rh6CG378500 Rh6DG364900
rosa_wichuraiana Rw6G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 5 cut(s) 4, 220, 704, 961, 1137
AclWI GGATC 1 cut(s) 834
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 4 cut(s) 73, 230, 880, 1086
AcuI CTGAAG 1 cut(s) 677
AfaI GTAC 4 cut(s) 16, 780, 860, 868
AfiI CCNNNNNNNGG 3 cut(s) 952, 1136, 1268
AgsI TTSAA 8 cut(s) 11, 51, 235, 430, 770, 821, 1105, 1351
AjnI CCWGG 3 cut(s) 17, 529, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
AluBI AGCT 5 cut(s) 93, 341, 935, 1025, 1057
AluI AGCT 5 cut(s) 93, 341, 935, 1025, 1057
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 136
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 1 cut(s) 834
Ama87I CYCGRG 1 cut(s) 732
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 2 cut(s) 20, 351
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 5 cut(s) 341, 503, 1013, 1016, 1322
ApoI RAATTY 4 cut(s) 73, 230, 880, 1086
Asp700I GAANNNNTTC 1 cut(s) 896
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 3 cut(s) 320, 454, 1140
AsuHPI GGTGA 2 cut(s) 52, 545
AvaI CYCGRG 1 cut(s) 732
AvaII GGWCC 3 cut(s) 320, 454, 1140
BaeGI GKGCMC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 1036
BauI CACGAG 2 cut(s) 381, 681
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 5 cut(s) 328, 515, 1000, 1003, 1309
BccI CCATC 4 cut(s) 311, 356, 1138, 1262
BceAI ACGGC 2 cut(s) 7, 338
BciT130I CCWGG 3 cut(s) 19, 531, 852
BciVI GTATCC 1 cut(s) 1137
BclI TGATCA 1 cut(s) 1209
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 1280
BfmI CTRYAG 2 cut(s) 930, 1323
BfuI GTATCC 1 cut(s) 1137
BisI GCNGC 6 cut(s) 342, 504, 962, 1014, 1017, 1323
BlsI GCNGC 6 cut(s) 343, 505, 963, 1015, 1018, 1324
Bme1390I CCNGG 3 cut(s) 19, 531, 852
Bme18I GGWCC 3 cut(s) 320, 454, 1140
BmeT110I CYCGRG 1 cut(s) 732
BmgT120I GGNCC 3 cut(s) 320, 454, 1140
BmiI GGNNCC 3 cut(s) 396, 456, 1141
BmrFI CCNGG 3 cut(s) 19, 531, 852
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 2 cut(s) 157, 764
BmuI ACTGGG 1 cut(s) 918
BpmI CTGGAG 2 cut(s) 612, 1073
BpuEI CTTGAG 1 cut(s) 109
BsaJI CCNNGG 4 cut(s) 114, 292, 588, 885
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bsc4I CCNNNNNNNGG 3 cut(s) 952, 1136, 1268
Bse1I ACTGG 6 cut(s) 445, 450, 721, 786, 913, 1242
Bse3DI GCAATG 2 cut(s) 414, 1013
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 3 cut(s) 19, 531, 852
BseDI CCNNGG 4 cut(s) 114, 292, 588, 885
BseGI GGATG 4 cut(s) 172, 1130, 1309, 1341
BseLI CCNNNNNNNGG 3 cut(s) 952, 1136, 1268
BseMI GCAATG 2 cut(s) 414, 1013
BseMII CTCAG 4 cut(s) 147, 411, 450, 1042
BseNI ACTGG 6 cut(s) 445, 450, 721, 786, 913, 1242
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 5 cut(s) 328, 515, 1000, 1003, 1309
BshFI GGCC 2 cut(s) 22, 353
BsiHKAI GWGCWC 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 732
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 3 cut(s) 10, 626, 1153
BslI CCNNNNNNNGG 3 cut(s) 952, 1136, 1268
BsmAI GTCTC 1 cut(s) 136
BsmFI GGGAC 3 cut(s) 10, 626, 1153
BsnI GGCC 2 cut(s) 22, 353
BsoBI CYCGRG 1 cut(s) 732
Bsp1286I GDGCHC 2 cut(s) 491, 1036
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 4 cut(s) 826, 918, 1159, 1209
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 5 cut(s) 4, 220, 704, 961, 1137
BspANI GGCC 2 cut(s) 22, 353
BspCNI CTCAG 4 cut(s) 146, 412, 451, 1041
BspEI TCCGGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 396, 456, 1141
BspMAI CTGCAG 1 cut(s) 1327
BspPI GGATC 1 cut(s) 834
BsrDI GCAATG 2 cut(s) 414, 1013
BsrI ACTGG 6 cut(s) 445, 450, 721, 786, 913, 1242
BssECI CCNNGG 4 cut(s) 114, 292, 588, 885
BssMI GATC 4 cut(s) 826, 918, 1159, 1209
BssSI CACGAG 2 cut(s) 381, 681
BssT1I CCWWGG 3 cut(s) 292, 588, 885
Bst2BI CACGAG 2 cut(s) 381, 681
Bst2UI CCWGG 3 cut(s) 19, 531, 852
Bst4CI ACNGT 4 cut(s) 118, 151, 475, 871
Bst6I CTCTTC 2 cut(s) 62, 852
BstC8I GCNNGC 1 cut(s) 966
BstDEI CTNAG 5 cut(s) 133, 420, 459, 1028, 1316
BstDSI CCRYGG 2 cut(s) 114, 588
BstF5I GGATG 4 cut(s) 172, 1130, 1309, 1341
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 4 cut(s) 829, 921, 1162, 1212
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 4 cut(s) 826, 918, 1159, 1209
BstMWI GCNNNNNNNGC 4 cut(s) 350, 503, 1022, 1031
BstNI CCWGG 3 cut(s) 19, 531, 852
BstSCI CCNGG 3 cut(s) 17, 529, 850
BstSFI CTRYAG 2 cut(s) 930, 1323
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 5 cut(s) 328, 515, 1000, 1003, 1309
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 2 cut(s) 22, 353
BtgI CCRYGG 2 cut(s) 114, 588
BtsCI GGATG 4 cut(s) 172, 1130, 1309, 1341
BtsIMutI CAGTG 3 cut(s) 438, 789, 1249
Cac8I GCNNGC 1 cut(s) 966
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 3 cut(s) 320, 454, 1140
CseI GACGC 1 cut(s) 755
Csp6I GTAC 4 cut(s) 15, 779, 859, 867
CviAII CATG 5 cut(s) 29, 278, 511, 589, 951
CviQI GTAC 4 cut(s) 15, 779, 859, 867
DdeI CTNAG 5 cut(s) 133, 420, 459, 1028, 1316
DpnI GATC 4 cut(s) 828, 920, 1161, 1211
DpnII GATC 4 cut(s) 826, 918, 1159, 1209
DraI TTTAAA 2 cut(s) 1120, 1257
EaeI YGGCCR 1 cut(s) 20
Eam1104I CTCTTC 2 cut(s) 62, 852
EarI CTCTTC 2 cut(s) 62, 852
Eco130I CCWWGG 3 cut(s) 292, 588, 885
Eco24I GRGCYC 1 cut(s) 1036
Eco47I GGWCC 3 cut(s) 320, 454, 1140
Eco57I CTGAAG 1 cut(s) 677
Eco88I CYCGRG 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 1140
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 3 cut(s) 17, 529, 850
EcoT14I CCWWGG 3 cut(s) 292, 588, 885
EcoT38I GRGCYC 1 cut(s) 1036
ErhI CCWWGG 3 cut(s) 292, 588, 885
FaeI CATG 5 cut(s) 32, 281, 514, 592, 954
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FaqI GGGAC 3 cut(s) 10, 626, 1153
FatI CATG 5 cut(s) 28, 277, 510, 588, 950
FauI CCCGC 1 cut(s) 1130
FbaI TGATCA 1 cut(s) 1209
Fnu4HI GCNGC 6 cut(s) 342, 504, 962, 1014, 1017, 1323
FokI GGATG 4 cut(s) 179, 1117, 1316, 1348
FriOI GRGCYC 1 cut(s) 1036
Fsp4HI GCNGC 6 cut(s) 342, 504, 962, 1014, 1017, 1323
FspBI CTAG 1 cut(s) 1280
GlaI GCGC 1 cut(s) 38
GluI GCNGC 6 cut(s) 342, 504, 962, 1014, 1017, 1323
GsuI CTGGAG 2 cut(s) 612, 1073
HaeIII GGCC 2 cut(s) 22, 353
HapII CCGG 1 cut(s) 323
HgaI GACGC 1 cut(s) 755
HhaI GCGC 1 cut(s) 39
Hin1II CATG 5 cut(s) 32, 281, 514, 592, 954
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 1 cut(s) 906
HindII GTYRAC 1 cut(s) 906
HinfI GANTC 7 cut(s) 7, 188, 426, 566, 580, 1040, 1276
HpaI GTTAAC 1 cut(s) 906
HpaII CCGG 1 cut(s) 323
HphI GGTGA 2 cut(s) 52, 545
Hpy166II GTNNAC 6 cut(s) 147, 454, 489, 729, 789, 906
Hpy188I TCNGA 4 cut(s) 1031, 1209, 1223, 1286
Hpy188III TCNNGA 7 cut(s) 235, 323, 398, 430, 629, 734, 1052
Hpy8I GTNNAC 6 cut(s) 147, 454, 489, 729, 789, 906
HpyAV CCTTC 3 cut(s) 135, 1075, 1099
HpyCH4III ACNGT 4 cut(s) 118, 151, 475, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 503, 1022, 1031
HpyF3I CTNAG 5 cut(s) 133, 420, 459, 1028, 1316
Hsp92II CATG 5 cut(s) 32, 281, 514, 592, 954
HspAI GCGC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 1209
KspAI GTTAAC 1 cut(s) 906
Kzo9I GATC 4 cut(s) 826, 918, 1159, 1209
LmnI GCTCC 4 cut(s) 281, 559, 940, 1054
Lsp1109I GCAGC 5 cut(s) 328, 515, 1000, 1003, 1309
LweI GCATC 2 cut(s) 157, 764
MaeI CTAG 1 cut(s) 1280
MaeIII GTNAC 2 cut(s) 475, 871
MalI GATC 4 cut(s) 828, 920, 1161, 1211
MboI GATC 4 cut(s) 826, 918, 1159, 1209
MboII GAAGA 8 cut(s) 39, 49, 238, 839, 928, 1277, 1351, 1354
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 2 cut(s) 491, 1036
MluCI AATT 7 cut(s) 73, 230, 345, 498, 687, 880, 1086
MlyI GAGTC 2 cut(s) 574, 1034
MmeI TCCRAC 2 cut(s) 204, 1201
MroI TCCGGA 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 4 cut(s) 273, 905, 1119, 1256
MslI CAYNNNNRTG 3 cut(s) 254, 836, 1001
MspA1I CMGCKG 1 cut(s) 1137
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 3 cut(s) 19, 531, 852
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 3 cut(s) 19, 531, 852
MwoI GCNNNNNNNGC 4 cut(s) 350, 503, 1022, 1031
NcoI CCATGG 1 cut(s) 588
NdeII GATC 4 cut(s) 826, 918, 1159, 1209
NlaIII CATG 5 cut(s) 32, 281, 514, 592, 954
NlaIV GGNNCC 3 cut(s) 396, 456, 1141
PaeR7I CTCGAG 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 5 cut(s) 7, 188, 426, 566, 1276
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 1 cut(s) 850
PkrI GCNGC 6 cut(s) 343, 505, 963, 1015, 1018, 1324
PleI GAGTC 2 cut(s) 574, 1034
PpsI GAGTC 2 cut(s) 574, 1034
PpuMI RGGWCCY 1 cut(s) 1140
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 1140
Psp6I CCWGG 3 cut(s) 17, 529, 850
PspGI CCWGG 3 cut(s) 17, 529, 850
PspN4I GGNNCC 3 cut(s) 396, 456, 1141
PspPI GGNCC 3 cut(s) 320, 454, 1140
PspPPI RGGWCCY 1 cut(s) 1140
PstI CTGCAG 1 cut(s) 1327
RsaI GTAC 4 cut(s) 16, 780, 860, 868
RsaNI GTAC 4 cut(s) 15, 779, 859, 867
RseI CAYNNNNRTG 3 cut(s) 254, 836, 1001
SaqAI TTAA 4 cut(s) 273, 905, 1119, 1256
SatI GCNGC 6 cut(s) 342, 504, 962, 1014, 1017, 1323
Sau3AI GATC 4 cut(s) 826, 918, 1159, 1209
Sau96I GGNCC 3 cut(s) 320, 454, 1140
SchI GAGTC 2 cut(s) 574, 1034
ScrFI CCNGG 3 cut(s) 19, 531, 852
SduI GDGCHC 2 cut(s) 491, 1036
SfaNI GCATC 2 cut(s) 157, 764
SfcI CTRYAG 2 cut(s) 930, 1323
Sfr274I CTCGAG 1 cut(s) 732
SinI GGWCC 3 cut(s) 320, 454, 1140
SlaI CTCGAG 1 cut(s) 732
SmiMI CAYNNNNRTG 3 cut(s) 254, 836, 1001
SmlI CTYRAG 2 cut(s) 88, 732
SmoI CTYRAG 2 cut(s) 88, 732
Sse9I AATT 7 cut(s) 73, 230, 345, 498, 687, 880, 1086
SsiI CCGC 5 cut(s) 4, 220, 704, 961, 1137
SspMI CTAG 1 cut(s) 1280
StyD4I CCNGG 3 cut(s) 17, 529, 850
StyI CCWWGG 3 cut(s) 292, 588, 885
TaaI ACNGT 4 cut(s) 118, 151, 475, 871
TaqI TCGA 3 cut(s) 155, 191, 733
TasI AATT 7 cut(s) 73, 230, 345, 498, 687, 880, 1086
TatI WGTACW 1 cut(s) 778
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 5 cut(s) 7, 188, 426, 566, 1276
Tru1I TTAA 4 cut(s) 273, 905, 1119, 1256
Tru9I TTAA 4 cut(s) 273, 905, 1119, 1256
TscAI CASTG 3 cut(s) 445, 796, 1249
TseI GCWGC 5 cut(s) 341, 503, 1013, 1016, 1322
TspDTI ATGAA 4 cut(s) 45, 666, 810, 1352
TspRI CASTG 3 cut(s) 445, 796, 1249
Van91I CCANNNNNTGG 1 cut(s) 1268
VneI GTGCAC 1 cut(s) 487
VpaK11BI GGWCC 3 cut(s) 320, 454, 1140
XapI RAATTY 4 cut(s) 73, 230, 880, 1086
XhoI CTCGAG 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 1 cut(s) 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.