FvH4_2g30910
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016560
Physical Location & Seq
Reverse (-)
23827480 .. 23835543
8064 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30910.t1

Sequence Viewer

Length: 2952 bp
ATGGCATTTCGGTTTGACTTCTTGCTGCTTCTGGGTTTGCTTGTTTGTGTGAGCTTTGGTAATGTGGATTCAGACGAAGGAGCAACACTGCTGGAGATAAAGAAATCGTTTAGGGATGAGGACAATGTTCTATACGACTGGAGAGACTCACCGTCTTTAGACTATTGTGCTTGGAGAGGCGTCACATGTGACAATGTCACCTTCAATGTCATAGCACTGAATCTATCAAGTCTAAATCTTGATGGGGAAATAGCACCTGCAATAGGAAACCTCAAGGGCCTCCAGTCCATAGACTTGAGAGGAAACCGACTTTCAGGCCAGATCCCAGATGAGATTGGTGACTGTTCGTCTCTGAGATACCTGGACCTGTCCTTTAACGAGATACATGGAGACATCCCATTTTCGATATCAAAGTTGAAGCAATTGGAATCCATAGTGTTGAAAAATAATCAGTTAATTGGACCACTCCCTTCAACACTCTCCCAGATTCCGAACCTGAAGACGTTAGACCTAGCCCAGAATAATCTCAGTGGGGAAATACCAAGGCTTATTTACTGGAATGAAGTTCTACAATATCTTGGTTTGCGAGGGAACAATTTAGTTGGGAAACTTTCTCCAGATATGTGCCAGTTAACTGGGTTATGGTATTTCGATGTGAGAAACAACAGTTTGACTGGTAGCATTCCTCAAAATATAGGGAACTGCACTGCCTTCCAAGTCTTGGATCTGTCCTATAACCAGCTAACTGGAGAGATTCCTTTTAACATTGGGTTCCTGCAAGTAGCGACCTTATCATTGCAGGGTAATCAACTCTCTGGGCCTATCCCTTCTGTGATTGGCCTGATGCAGGCACTTGCTGTGTTAGATTTGAGCGGCAATATGCTAAGTGGGCCGATTCCTCCTATTCTGGGGAATTTGACTTATACAGAGAAATTGTATTTGCATGCCAACAAGCTTACAGGATCAATTCCCCCAGAGCTTGGACAAATGGAACAGCTTCATTATTTGGTGTTGAATGACAACCATCTTACTGGACAAATTCCACCTGAACTTGGGAAGCTTACGAACTTGTTTGACCTAAATGTTGCTGACAACAATCTTCAAGGGCCCATTCCTGATAATCTTAGCTCATGTACCAATCTCAACACCCTCAATGTGCATGGGAATAAATTGAATGGAACCATCCCTCCTGCTCTCCAGAGGCTGGAGAGTTTGACTTATCTTAATCTGTCCTCCAACAGTCTTCATGGCTCCATACCAATTGAGCTATCTCGGATTGGCAATTTGGATACTTTGGATCTTTCTGACAACAAGTTTAGTGGTGCCATACCTTCTTCGCTTGGTGATTTGGAACATCTTCTTAAGCTGAATTTGAGTAGAAATCATATGACAGGATTTATTCCGGCAGAGTTTGGTAATCTAAGGAGTGTTGTGGACATAGATCTTTCAAATAATCAGCTCACAGGAATGATTCCTCAGGAGCTCAGTCAGCTGCAGAATTTGTTCGCACTGAGGCTAGAGCACAACAATATATCAGGGGAAATGGTGTCTTTGATCAATTGCCTCAGTCTCGCAGTGCTAAATGTTTCTTATAACAACCTGGCGGGAGATATTCCCACATCCAATAACTTCTCAAGGTTTTCACCCGACAGTTTTGTTGGAAATCCTGATCTTTGTGGCTATTGGCTCAATTCTCCGTGTCACGAGTCCCGTCCAACAGAGAGAGTTGCATTATCTAAAGCAGCTATCCTGGGAATTGCCCTTGGTGCCCTTGTGATTCTTCTCATGATCCTTATTGCCGCCTGCCGTCCTTACCATCCAACTCCTTTCCCTGATGGTTCACTTGACAAACCAGTTAATTACTCCACACCAAAGCTAGTGATTTTAAACATGAATATGGCACTTCATATCTATGAGGATATCATGAGGATGACTGAAAACTTGAGTGAAAAGTATATAATTGGTTATGGCGCATCAAGCACGGTATACAAATGTGTTCTTAAGAATTGTAAGCCAGTAGCGATCAAGAGACTCTACTCTCATCATCCACCGTGCTTGAAGGAGTTTGAGACAGAACTTGAAACAGTTGGCAGTATCAAGCACCGAAATCTGGTCAGCCTCCAAGGATACTCCTTGTCTTCTTCTGGAAACCTTCTCTTTTATGATTACATGGAAAATGGCAGTCTCTGGGATCTCCTTCATGGCCCAACTACCAAGAAGAAAAAGCTCGACTGGGATACTCGTGTCCAGATAGCACTTGGAGCAGCCCAAGGGCTTGCCTATCTCCATCATGATTGTAGTCCACGCATCATACACAGGGATGTGAAGTCGTCTAACATTCTGTTGGACAAGGATTTTGAGGCTCATCTAACTGATTTTGGTATTGCCAAGAGCTTATGCATTTCAAAGACCCATACATCTACCTGTGTGATGGGTACTATTGGCTACATAGACCCCGAGTATGCTCGAACTTCCCGCCTTACTGAGAAATCAGACGTTTATAGTTATGGGATTGTCCTGCTGGAGTTGCTAACAGGAAGGAAAGCTGTAGATAATGAATGCAATCTTCATCATTTGATCTTATCCAAGACAGCGAACAATGCTGTGATGGAAACTGTTGATCCGGAGATCACGTCCACATGCATGGACCTTGGAGCAGTGAAGAAGGTTTTTCAGCTTGCACTCTTATGCACAAAGAGGCAGCCAACAGACCGGCCGACTATGCATGAAGTAACAAGAGTTTTGGGTAGCCTTGTGCCATCACCTACACAGCCGAAACAAGCAACCCTAAATCCACTATCCATTACACTCTCATCTGTCAAAGCGCCGTGCTACATGGATGAGTATGCAAACCTCAAAACCCCACACATGCTCAATTGCCCGTCTATGAGTACCTCGGACGCACAGCTCTTTCTCAAGTTTGGAGAGGTAATTTCTCAAAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

984

Amino Acids

108.21

Weight (kDa)

5.6

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 64 4.6e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 85 - 172 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 87 - 128 3.5e-06 Leucine rich repeat
LRR_4 PF12799 117 - 156 4.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 264 - 388 1.8e-06 Leucine-rich repeat region
LRR_8 PF13855 356 - 415 9.4e-06 Leucine rich repeat
LRR_14 PF23598 375 - 522 2.7e-10 Leucine-rich repeat region
LRR_8 PF13855 428 - 487 8.4e-10 Leucine rich repeat
LRR_8 PF13855 452 - 511 2e-08 Leucine rich repeat
Pkinase PF00069 651 - 912 1.7e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 653 - 914 2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AarI CACCTGC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 2 cut(s) 1322, 1766
AccB7I CCANNNNNTGG 3 cut(s) 721, 980, 1204
AccBSI CCGCTC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 1986
AccIII TCCGGA 1 cut(s) 2623
AciI CCGC 4 cut(s) 873, 1604, 1800, 2476
AclWI GGATC 7 cut(s) 316, 732, 970, 1305, 1783, 2199, 2615
AcoI YGGCCR 1 cut(s) 2714
AcsI RAATTY 4 cut(s) 913, 1038, 1369, 1498
AcuI CTGAAG 1 cut(s) 518
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 1135, 2437, 2893
AfiI CCNNNNNNNGG 8 cut(s) 263, 721, 847, 908, 980, 1052, 1204, 2110
AflII CTTAAG 2 cut(s) 1361, 2000
AflIII ACRYGT 1 cut(s) 185
AhdI GACNNNNNGTC 2 cut(s) 151, 346
AjiI CACGTC 1 cut(s) 2634
AjnI CCWGG 3 cut(s) 360, 1599, 1749
AloI GAACNNNNNNTCC 2 cut(s) 1171, 1203
Alw21I GWGCWC 2 cut(s) 1485, 1524
Alw26I GTCTC 7 cut(s) 138, 354, 384, 1574, 2023, 2063, 2189
AlwI GGATC 7 cut(s) 316, 732, 970, 1305, 1783, 2199, 2615
AlwNI CAGNNNCTG 2 cut(s) 1204, 2187
Ama87I CYCGRG 1 cut(s) 2456
Aor13HI TCCGGA 1 cut(s) 2623
AoxI GGCC 8 cut(s) 277, 316, 818, 838, 890, 1106, 2203, 2714
ApaI GGGCCC 1 cut(s) 1110
ApeKI GCWGC 5 cut(s) 25, 1492, 1742, 2264, 2701
ApoI RAATTY 4 cut(s) 913, 1038, 1369, 1498
ArsI GACNNNNNNTTYG 2 cut(s) 2339, 2371
Asp700I GAANNNNTTC 3 cut(s) 996, 1356, 1502
AspLEI GCGC 2 cut(s) 1973, 2827
AspS9I GGNCC 9 cut(s) 277, 364, 461, 818, 890, 1106, 1107, 2204, 2647
AsuHPI GGTGA 6 cut(s) 141, 190, 350, 1355, 1635, 2754
AvaI CYCGRG 1 cut(s) 2456
AvaII GGWCC 3 cut(s) 364, 461, 2647
AxyI CCTNAGG 1 cut(s) 1476
BaeGI GKGCMC 2 cut(s) 1110, 1771
BaeI ACNNNNGTAYC 2 cut(s) 2229, 2262
BanI GGYRCC 2 cut(s) 1322, 1766
BanII GRGCYC 2 cut(s) 1110, 1485
BauI CACGAG 2 cut(s) 1703, 2241
BbsI GAAGAC 3 cut(s) 506, 1235, 2130
Bbv12I GWGCWC 2 cut(s) 1485, 1524
BbvI GCAGC 5 cut(s) 12, 1479, 1754, 2276, 2713
BccI CCATC 9 cut(s) 236, 1032, 1190, 1824, 1829, 2295, 2425, 2602, 2767
BceAI ACGGC 2 cut(s) 1791, 2812
BciT130I CCWGG 3 cut(s) 362, 1601, 1751
BciVI GTATCC 3 cut(s) 1282, 2120, 2230
BclI TGATCA 1 cut(s) 1554
BcoDI GTCTC 7 cut(s) 138, 354, 384, 1574, 2023, 2063, 2189
BfaI CTAG 3 cut(s) 512, 1517, 1877
BfmI CTRYAG 2 cut(s) 1493, 2547
BfoI RGCGCY 1 cut(s) 2828
BfrI CTTAAG 2 cut(s) 1361, 2000
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 3 cut(s) 1282, 2120, 2230
BglII AGATCT 1 cut(s) 1441
BisI GCNGC 7 cut(s) 26, 874, 1493, 1743, 1800, 2265, 2702
BlsI GCNGC 7 cut(s) 27, 875, 1494, 1744, 1801, 2266, 2703
Bme1390I CCNGG 3 cut(s) 362, 1601, 1751
Bme18I GGWCC 3 cut(s) 364, 461, 2647
BmeRI GACNNNNNGTC 2 cut(s) 151, 346
BmeT110I CYCGRG 1 cut(s) 2456
BmgBI CACGTC 1 cut(s) 2634
BmgT120I GGNCC 9 cut(s) 277, 364, 461, 818, 890, 1106, 1107, 2204, 2647
BmiI GGNNCC 6 cut(s) 773, 1108, 1180, 1252, 1324, 1768
BmrFI CCNGG 3 cut(s) 362, 1601, 1751
BmrI ACTGGG 2 cut(s) 645, 2242
BmsI GCATC 3 cut(s) 834, 1982, 2316
BmuI ACTGGG 2 cut(s) 645, 2242
BpiI GAAGAC 3 cut(s) 506, 1235, 2130
BpmI CTGGAG 8 cut(s) 113, 160, 266, 600, 768, 1181, 1226, 2543
BpuEI CTTGAG 5 cut(s) 257, 316, 1618, 1963, 2900
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 7 cut(s) 542, 1750, 1762, 2122, 2269, 2650, 2895
BsaWI WCCGGW 1 cut(s) 2623
BsaXI ACNNNNNCTCC 4 cut(s) 1171, 1201, 2618, 2648
Bsc4I CCNNNNNNNGG 8 cut(s) 263, 721, 847, 908, 980, 1052, 1204, 2110
Bse118I RCCGGY 1 cut(s) 2712
Bse21I CCTNAGG 1 cut(s) 1476
Bse3DI GCAATG 1 cut(s) 794
BseAI TCCGGA 1 cut(s) 2623
BseBI CCWGG 3 cut(s) 362, 1601, 1751
BseDI CCNNGG 7 cut(s) 542, 1750, 1762, 2122, 2269, 2650, 2895
BseGI GGATG 9 cut(s) 121, 393, 1182, 1619, 1816, 1935, 2044, 2326, 2845
BseLI CCNNNNNNNGG 8 cut(s) 263, 721, 847, 908, 980, 1052, 1204, 2110
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 7 cut(s) 344, 541, 1490, 1498, 1502, 1579, 2475
BseSI GKGCMC 2 cut(s) 1110, 1771
BseX3I CGGCCG 1 cut(s) 2714
BseXI GCAGC 5 cut(s) 12, 1479, 1754, 2276, 2713
BsgI GTGCAG 1 cut(s) 688
Bsh1285I CGRYCG 1 cut(s) 2717
BshFI GGCC 8 cut(s) 279, 318, 820, 840, 892, 1108, 2205, 2716
BshNI GGYRCC 2 cut(s) 1322, 1766
BsiEI CGRYCG 1 cut(s) 2717
BsiHKAI GWGCWC 2 cut(s) 1485, 1524
BsiHKCI CYCGRG 1 cut(s) 2456
BsiSI CCGG 3 cut(s) 1403, 2624, 2713
BslFI GGGAC 1 cut(s) 1693
BslI CCNNNNNNNGG 8 cut(s) 263, 721, 847, 908, 980, 1052, 1204, 2110
BsmAI GTCTC 7 cut(s) 138, 354, 384, 1574, 2023, 2063, 2189
BsmBI CGTCTC 1 cut(s) 354
BsmFI GGGAC 1 cut(s) 1693
BsmI GAATGC 2 cut(s) 681, 2564
BsnI GGCC 8 cut(s) 279, 318, 820, 840, 892, 1108, 2205, 2716
BsoBI CYCGRG 1 cut(s) 2456
Bsp120I GGGCCC 1 cut(s) 1106
Bsp1286I GDGCHC 4 cut(s) 1110, 1485, 1524, 1771
Bsp13I TCCGGA 1 cut(s) 2623
BspACI CCGC 4 cut(s) 873, 1604, 1800, 2476
BspANI GGCC 8 cut(s) 279, 318, 820, 840, 892, 1108, 2205, 2716
BspCNI CTCAG 7 cut(s) 345, 540, 1489, 1497, 1503, 1578, 2476
BspEI TCCGGA 1 cut(s) 2623
BspHI TCATGA 3 cut(s) 1785, 1923, 2290
BspLI GGNNCC 6 cut(s) 773, 1108, 1180, 1252, 1324, 1768
BspMAI CTGCAG 1 cut(s) 1497
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 7 cut(s) 316, 732, 970, 1305, 1783, 2199, 2615
BspT107I GGYRCC 2 cut(s) 1322, 1766
BspTI CTTAAG 2 cut(s) 1361, 2000
BsrBI CCGCTC 1 cut(s) 873
BsrDI GCAATG 1 cut(s) 794
BsrFI RCCGGY 1 cut(s) 2712
BssAI RCCGGY 1 cut(s) 2712
BssECI CCNNGG 7 cut(s) 542, 1750, 1762, 2122, 2269, 2650, 2895
BssNAI GTATAC 1 cut(s) 1987
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 2 cut(s) 1703, 2241
BssT1I CCWWGG 5 cut(s) 542, 1762, 2122, 2269, 2650
Bst1107I GTATAC 1 cut(s) 1987
Bst2BI CACGAG 2 cut(s) 1703, 2241
Bst2UI CCWGG 3 cut(s) 362, 1601, 1751
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 2 cut(s) 1361, 2000
BstC8I GCNNGC 5 cut(s) 849, 945, 1804, 2277, 2679
BstENI CCTNNNNNAGG 2 cut(s) 261, 845
BstF5I GGATG 9 cut(s) 121, 393, 1182, 1619, 1816, 1935, 2044, 2326, 2845
BstH2I RGCGCY 1 cut(s) 2828
BstHHI GCGC 2 cut(s) 1973, 2827
BstMAI GTCTC 7 cut(s) 138, 354, 384, 1574, 2023, 2063, 2189
BstMCI CGRYCG 1 cut(s) 2717
BstMWI GCNNNNNNNGC 8 cut(s) 889, 1489, 1766, 1977, 2261, 2527, 2600, 2722
BstNI CCWGG 3 cut(s) 362, 1601, 1751
BstNSI RCATGY 4 cut(s) 189, 947, 2643, 2872
BstSCI CCNGG 3 cut(s) 360, 1599, 1749
BstSFI CTRYAG 2 cut(s) 1493, 2547
BstSLI GKGCMC 2 cut(s) 1110, 1771
BstV1I GCAGC 5 cut(s) 12, 1479, 1754, 2276, 2713
BstV2I GAAGAC 3 cut(s) 506, 1235, 2130
BstX2I RGATCY 5 cut(s) 321, 724, 1297, 1441, 2191
BstXI CCANNNNNNTGG 4 cut(s) 635, 746, 1031, 2644
BstYI RGATCY 5 cut(s) 321, 724, 1297, 1441, 2191
BstZ17I GTATAC 1 cut(s) 1987
BstZI CGGCCG 1 cut(s) 2714
Bsu36I CCTNAGG 1 cut(s) 1476
BsuI GTATCC 3 cut(s) 1282, 2120, 2230
BsuRI GGCC 8 cut(s) 279, 318, 820, 840, 892, 1108, 2205, 2716
BtrI CACGTC 1 cut(s) 2634
BtsCI GGATG 9 cut(s) 121, 393, 1182, 1619, 1816, 1935, 2044, 2326, 2845
BtsI GCAGTG 4 cut(s) 86, 705, 1581, 2664
BtsIMutI CAGTG 8 cut(s) 86, 215, 535, 705, 1508, 1581, 2664, 2950
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 5 cut(s) 849, 945, 1804, 2277, 2679
CaiI CAGNNNCTG 2 cut(s) 1204, 2187
CciI TCATGA 3 cut(s) 1785, 1923, 2290
CfoI GCGC 2 cut(s) 1973, 2827
Cfr10I RCCGGY 1 cut(s) 2712
Cfr13I GGNCC 9 cut(s) 277, 364, 461, 818, 890, 1106, 1107, 2204, 2647
CseI GACGC 2 cut(s) 169, 2909
Csp6I GTAC 3 cut(s) 1134, 2436, 2892
CspCI CAANNNNNGTGG 4 cut(s) 1300, 1335, 2037, 2072
CviQI GTAC 3 cut(s) 1134, 2436, 2892
DraI TTTAAA 1 cut(s) 1887
DriI GACNNNNNGTC 2 cut(s) 151, 346
EaeI YGGCCR 1 cut(s) 2714
EagI CGGCCG 1 cut(s) 2714
Eam1105I GACNNNNNGTC 2 cut(s) 151, 346
Ecl136II GAGCTC 1 cut(s) 1483
EclXI CGGCCG 1 cut(s) 2714
Eco130I CCWWGG 5 cut(s) 542, 1762, 2122, 2269, 2650
Eco24I GRGCYC 2 cut(s) 1110, 1485
Eco32I GATATC 2 cut(s) 408, 1921
Eco47I GGWCC 3 cut(s) 364, 461, 2647
Eco52I CGGCCG 1 cut(s) 2714
Eco53kI GAGCTC 1 cut(s) 1483
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 1476
Eco88I CYCGRG 1 cut(s) 2456
EcoICRI GAGCTC 1 cut(s) 1483
EcoNI CCTNNNNNAGG 2 cut(s) 261, 845
EcoO109I RGGNCCY 2 cut(s) 277, 1106
EcoRII CCWGG 3 cut(s) 360, 1599, 1749
EcoRV GATATC 2 cut(s) 408, 1921
EcoT14I CCWWGG 5 cut(s) 542, 1762, 2122, 2269, 2650
EcoT22I ATGCAT 3 cut(s) 2402, 2645, 2727
EcoT38I GRGCYC 2 cut(s) 1110, 1485
ErhI CCWWGG 5 cut(s) 542, 1762, 2122, 2269, 2650
Esp3I CGTCTC 1 cut(s) 354
FaqI GGGAC 1 cut(s) 1693
FauI CCCGC 2 cut(s) 1597, 2483
FauNDI CATATG 1 cut(s) 1386
FbaI TGATCA 1 cut(s) 1554
FblI GTMKAC 1 cut(s) 1986
Fnu4HI GCNGC 7 cut(s) 26, 874, 1493, 1743, 1800, 2265, 2702
FokI GGATG 9 cut(s) 128, 380, 1169, 1606, 1803, 1942, 2031, 2333, 2852
FriOI GRGCYC 2 cut(s) 1110, 1485
Fsp4HI GCNGC 7 cut(s) 26, 874, 1493, 1743, 1800, 2265, 2702
FspBI CTAG 3 cut(s) 512, 1517, 1877
GlaI GCGC 2 cut(s) 1972, 2826
GluI GCNGC 7 cut(s) 26, 874, 1493, 1743, 1800, 2265, 2702
GsuI CTGGAG 8 cut(s) 113, 160, 266, 600, 768, 1181, 1226, 2543
HaeII RGCGCY 1 cut(s) 2828
HaeIII GGCC 8 cut(s) 279, 318, 820, 840, 892, 1108, 2205, 2716
HapII CCGG 3 cut(s) 1403, 2624, 2713
HgaI GACGC 2 cut(s) 169, 2909
HhaI GCGC 2 cut(s) 1973, 2827
Hin1I GRCGYC 1 cut(s) 180
Hin6I GCGC 2 cut(s) 1971, 2825
HinP1I GCGC 2 cut(s) 1971, 2825
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HindIII AAGCTT 2 cut(s) 953, 1058
HpaI GTTAAC 1 cut(s) 633
HpaII CCGG 3 cut(s) 1403, 2624, 2713
HphI GGTGA 6 cut(s) 141, 190, 350, 1355, 1635, 2754
Hpy166II GTNNAC 6 cut(s) 633, 1435, 1841, 1987, 2303, 2637
Hpy188I TCNGA 7 cut(s) 73, 354, 492, 1275, 1306, 2494, 2899
Hpy8I GTNNAC 6 cut(s) 633, 1435, 1841, 1987, 2303, 2637
HpyCH4IV ACGT 3 cut(s) 503, 2496, 2633
HpyF10VI GCNNNNNNNGC 8 cut(s) 889, 1489, 1766, 1977, 2261, 2527, 2600, 2722
HpySE526I ACGT 3 cut(s) 503, 2496, 2633
Hsp92I GRCGYC 1 cut(s) 180
HspAI GCGC 2 cut(s) 1971, 2825
Kpn2I TCCGGA 1 cut(s) 2623
Ksp22I TGATCA 1 cut(s) 1554
KspAI GTTAAC 1 cut(s) 633
LmnI GCTCC 5 cut(s) 80, 1256, 1480, 2261, 2654
Lsp1109I GCAGC 5 cut(s) 12, 1479, 1754, 2276, 2713
LweI GCATC 3 cut(s) 834, 1982, 2316
MaeI CTAG 3 cut(s) 512, 1517, 1877
MaeII ACGT 3 cut(s) 503, 2496, 2633
MaeIII GTNAC 6 cut(s) 181, 188, 196, 338, 1700, 2731
MbiI CCGCTC 1 cut(s) 873
MfeI CAATTG 4 cut(s) 422, 1260, 1558, 2875
MflI RGATCY 5 cut(s) 321, 724, 1297, 1441, 2191
MhlI GDGCHC 4 cut(s) 1110, 1485, 1524, 1771
MlyI GAGTC 3 cut(s) 140, 1715, 2025
MmeI TCCRAC 5 cut(s) 1260, 1639, 1739, 1844, 2325
Mph1103I ATGCAT 3 cut(s) 2402, 2645, 2727
MroI TCCGGA 1 cut(s) 2623
MroXI GAANNNNTTC 3 cut(s) 996, 1356, 1502
MseI TTAA 9 cut(s) 375, 455, 632, 762, 1224, 1362, 1857, 1886, 2001
MslI CAYNNNNRTG 7 cut(s) 1466, 1895, 1911, 1928, 2319, 2642, 2686
MspA1I CMGCKG 1 cut(s) 1492
MspCI CTTAAG 2 cut(s) 1361, 2000
MspI CCGG 3 cut(s) 1403, 2624, 2713
MspR9I CCNGG 3 cut(s) 362, 1601, 1751
MunI CAATTG 4 cut(s) 422, 1260, 1558, 2875
Mva1269I GAATGC 2 cut(s) 681, 2564
MvaI CCWGG 3 cut(s) 362, 1601, 1751
MwoI GCNNNNNNNGC 8 cut(s) 889, 1489, 1766, 1977, 2261, 2527, 2600, 2722
NdeI CATATG 1 cut(s) 1386
NlaIV GGNNCC 6 cut(s) 773, 1108, 1180, 1252, 1324, 1768
NmuCI GTSAC 5 cut(s) 181, 188, 196, 338, 1700
NsiI ATGCAT 3 cut(s) 2402, 2645, 2727
NspI RCATGY 4 cut(s) 189, 947, 2643, 2872
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 3 cut(s) 1785, 1923, 2290
PaqCI CACCTGC 1 cut(s) 265
PciI ACATGT 1 cut(s) 185
PctI GAATGC 2 cut(s) 681, 2564
PdmI GAANNNNTTC 3 cut(s) 996, 1356, 1502
PfeI GAWTC 8 cut(s) 68, 220, 428, 487, 754, 895, 1471, 1777
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 3 cut(s) 721, 980, 1204
PkrI GCNGC 7 cut(s) 27, 875, 1494, 1744, 1801, 2266, 2703
PleI GAGTC 3 cut(s) 140, 1714, 2025
PpsI GAGTC 3 cut(s) 140, 1714, 2025
PscI ACATGT 1 cut(s) 185
PsiI TTATAA 1 cut(s) 1593
Psp124BI GAGCTC 1 cut(s) 1485
Psp6I CCWGG 3 cut(s) 360, 1599, 1749
PspGI CCWGG 3 cut(s) 360, 1599, 1749
PspN4I GGNNCC 6 cut(s) 773, 1108, 1180, 1252, 1324, 1768
PspOMI GGGCCC 1 cut(s) 1106
PspPI GGNCC 9 cut(s) 277, 364, 461, 818, 890, 1106, 1107, 2204, 2647
PstI CTGCAG 1 cut(s) 1497
PstNI CAGNNNCTG 2 cut(s) 1204, 2187
PsuI RGATCY 5 cut(s) 321, 724, 1297, 1441, 2191
PsyI GACNNNGTC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 1492
RsaI GTAC 3 cut(s) 1135, 2437, 2893
RsaNI GTAC 3 cut(s) 1134, 2436, 2892
RseI CAYNNNNRTG 7 cut(s) 1466, 1895, 1911, 1928, 2319, 2642, 2686
SacI GAGCTC 1 cut(s) 1485
SaqAI TTAA 9 cut(s) 375, 455, 632, 762, 1224, 1362, 1857, 1886, 2001
SatI GCNGC 7 cut(s) 26, 874, 1493, 1743, 1800, 2265, 2702
Sau96I GGNCC 9 cut(s) 277, 364, 461, 818, 890, 1106, 1107, 2204, 2647
SchI GAGTC 3 cut(s) 140, 1715, 2025
ScrFI CCNGG 3 cut(s) 362, 1601, 1751
SduI GDGCHC 4 cut(s) 1110, 1485, 1524, 1771
SfaNI GCATC 3 cut(s) 834, 1982, 2316
SfcI CTRYAG 2 cut(s) 1493, 2547
SinI GGWCC 3 cut(s) 364, 461, 2647
SmiMI CAYNNNNRTG 7 cut(s) 1466, 1895, 1911, 1928, 2319, 2642, 2686
SmlI CTYRAG 7 cut(s) 272, 295, 1361, 1633, 1942, 2000, 2915
SmoI CTYRAG 7 cut(s) 272, 295, 1361, 1633, 1942, 2000, 2915
SphI GCATGC 1 cut(s) 947
SsiI CCGC 4 cut(s) 873, 1604, 1800, 2476
SspMI CTAG 3 cut(s) 512, 1517, 1877
SstI GAGCTC 1 cut(s) 1485
StyD4I CCNGG 3 cut(s) 360, 1599, 1749
StyI CCWWGG 5 cut(s) 542, 1762, 2122, 2269, 2650
TaiI ACGT 3 cut(s) 506, 2499, 2636
TaqI TCGA 4 cut(s) 404, 651, 2229, 2467
TauI GCSGC 2 cut(s) 876, 1802
TfiI GAWTC 8 cut(s) 68, 220, 428, 487, 754, 895, 1471, 1777
Tru1I TTAA 9 cut(s) 375, 455, 632, 762, 1224, 1362, 1857, 1886, 2001
Tru9I TTAA 9 cut(s) 375, 455, 632, 762, 1224, 1362, 1857, 1886, 2001
TscAI CASTG 8 cut(s) 93, 222, 535, 712, 1515, 1581, 2664, 2950
TseFI GTSAC 5 cut(s) 181, 188, 196, 338, 1700
TseI GCWGC 5 cut(s) 25, 1492, 1742, 2264, 2701
Tsp45I GTSAC 5 cut(s) 181, 188, 196, 338, 1700
TspDTI ATGAA 9 cut(s) 576, 989, 1235, 1895, 1907, 2189, 2558, 2571, 2742
TspGWI ACGGA 1 cut(s) 1686
TspRI CASTG 8 cut(s) 93, 222, 535, 712, 1515, 1581, 2664, 2950
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 3 cut(s) 721, 980, 1204
Vha464I CTTAAG 2 cut(s) 1361, 2000
VpaK11BI GGWCC 3 cut(s) 364, 461, 2647
XagI CCTNNNNNAGG 2 cut(s) 261, 845
XapI RAATTY 4 cut(s) 913, 1038, 1369, 1498
XceI RCATGY 4 cut(s) 189, 947, 2643, 2872
XcmI CCANNNNNNNNNTGG 1 cut(s) 2255
XmiI GTMKAC 1 cut(s) 1986
XmnI GAANNNNTTC 3 cut(s) 996, 1356, 1502
XspI CTAG 3 cut(s) 512, 1517, 1877
Zsp2I ATGCAT 3 cut(s) 2402, 2645, 2727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.