MD08G1071900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016560
Physical Location & Seq
Forward (+)
5712576 .. 5719779
7204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1071900.v1.1.491

Sequence Viewer

Length: 2979 bp
ATGAGGTTTGATGGGAGGGCAGTAATGGCATTTCGGGTGGAGTTTGTGCTTCTGGTTCTTCTTCTTGGGTGTGTGAGCTTCGGTTATGGGGACTCACAGAACGATCAAGGAAGAACACTGCTGGGGATAAAGAAGGCATTTAGGGATGTGGACAATGTTCTCTATGAATGGACAGATTCACCCTCTTTAGATTACTGTGTTTGGAGAGGCGTCACATGTGATAACTCTCTCAATGTCATTGCACTTAATCTATCAGGTTTGAATCTTGGAGGGGAAATCTCATCTGTGATCGGAGATCTCGAAAGCCTGCAGTCTATTGATTTGAGGGGAAACCACCTTTCCGGCCAGATCCCGGATGAGATTGGTGACTGTGTAGCACTGCGAAACCTGGACTTGTCCTTCAATGAGATATTTGGAGACATACCATTTTCGATATCCAAGTTGAAACAGCTTGAAAATCTGATTTTAAAAAATAATCAATTGATCGGACCACTTCCTTCAACGCTGTCCCAGATTCCAAACCTGAAGATTTTAGACCTAGCACAGAATAATCTCAGTGGGGAGATACCAAGGCTTATTTACTGGAATGAAGTTCTACAGTATCTTGGCTTGCGAGGGAACAATTTAGTTGGAACACTTTCTCCAGATATGTGCCAGTTAACTGGGTTATGGTATTTTGATGTGAGAAATAACAGCTTGACCGGTAGCATTCCTCAAAACATAGGGAACTGCACTGCCTTCCAGGTTTTGGATCTATCCTACAACCAGCTAACTGGAGGGATTCCGTTTAATATCGGGTTTCTGCAAGTTGCGACCTTGTCATTACAAGGTAATCAACTTTCTGGGCCTATCCCATCCGTGATTGGCCTGATGCAGGCTCTCGCTGTCTTGGATTTGAGCAGCAACGCTTTAAGTGGACCAATCCCTCCTATCCTAGGGAATTTGACTTATACAGAGAAACTGTATTTGCATGCTAACAAGCTTAATGGATCAATCCCCCCAGAGCTTGGACAGATGTCAAAGCTTCATTATTTGGAGTTGAATGATAACCATCTTACAGGACATATTCCACCGGAACTTGGGAAGCTGACCGATTTGTATGACCTTAACGTTGCGAACAACTATCTTCAAGGACCTATCCCTGATAATCTCAGCTCGTGTACAAATCTCAACAGCCTCAATGTGCATGGGAACAAGTTGAGTGGAACCATCCCGCCTGCTCTTCAGAGGCTGGAGAGTATGACTTACTTAAATCTGTCCTCCAACTATCTTCGTGGTCCAATTCCTATCGAGCTGTCTTTTATTGGTAACTTGGATACCTTGGATATTTCAAATAACAAAATAAGTGGAACCATTCCTTCATCACTTGGGGATTTGGAACATCTTCTAAAGCTGAATTTGAGTCGAAACCATTTAACGGGATTTATTCCAGGGGAGTTTGGTAATTTAAGGAGTGTTATGGAAATAGACCTTTCAAATAATCAGCTCACAGGATTAATTCCTCGAGAGCTCAGTCAGCTACAGAACATGAATTTATTGAGATTAGAGCACAACAATATATCCGGGGATGTGGTATCACTGATAAACTGCTTCAGCCTTTCTGTACTAAATGTATCTTACAACAACCTGGCAGGCGACGTTCCCACGAGCAAGAACTTCTCAAGGTTTTCACCAGACAGTTTTATTGGAAATCCTGATCTTTGTGGCCCTTGGCTCGATTCTCGATGTCATGAGTCTCGTCCAACAGAGCGAGCCACACTATCTAAACCTGCTATCCTGGGAATTGCTCTTGGGGCCCTTGTGATTCTTCTCATGATTCTTATTGCTGTATGCCGGCCATATAATCCAACTCCTTTTTCTGATGGTTCATTTGACAAACCGGTTAATTACTCAAATCCGAAGCTGGTGATCCTTCACATGAATTTGGCACTTCATGTGTACGAGGACATCATGAGGATGACTGAAAACTTGAGCGAGAAGTATATAATTGGTTACGGTGCATCAAGTACAGTGTACAAATGTGTTCTGAAAAATTGTAAGCCAGTGGCCATCAAGAAACTCTACACCCAGTATCATCGGTGCATGAAGGAATTTGAGACAGAACTTGCGACAGTTGGAAGCATCAAGCATCGGAATCTGGTGAGCCTCCAGGGGTACTCCTTGTCCTCCTCCGGAAACCTTCTCTTTTACGACTACATGGAAAATGGCAGTCTCTGGGATCACCTTCATGGCCCTTTCAAGAAGAAGAAGCTCGACTGGACAACTCGTCTCCAGATTGCCCTTGGAGCAGCCCAAGGGCTTGCCTATCTGCATCATGATTGCAGCCCCCGAATCATACACCGGGATGTCAAGTCGTCCAATATTCTATTGGACAAGGATTTCGAAGCTCATCTAAATGATTTCGGCATTGCCAAGACCTTATGCCACTCAAAGACCCATACGTCTACTTACTTAATGGGCACGATTGGCTACATAGACCCTGAGTATGCGCGAACTTCCCGCCTCACTGAGAAGTCCGATGTGTATAGTTATGGAATTGTTCTGCTGGAGTTGCTGACAGGAAGAAAAGCCGTAGACAATGAATCCAATCTCCATCATTTGATATTATCCAAGACGGAGAGCAATGCTGTCATGGAAACCGTAGACCCCGAGATCTTGCCAACATGCACAGACCTCGGACCGGTGAAGAAGGTCTTCCAGCTTGCCCTTCTGTGCACAAAGCGGCAGCCAACAGACCGGCCAACAATGCACGAAGTAACTCGCGTGTTGGCCAGTCTCATGCCTTGCCCCGCACCACCAAAACAACCAACCCCTGCCAACCCGCCATCGTCACAGCTCCCGATCACCAAAGTGCCGTGCTACGTGGACGAGTACGTGAATCTGAAAACGCCACACATGCTGAATTGTCCATCAATGAGCACCTCAGATGCCCAGCTGTTTCTCAAGTTTGGAGAGGTAATTTCTCAGAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

993

Amino Acids

109.57

Weight (kDa)

5.96

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 74 3.8e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 89 - 181 8.3e-06 Leucine-rich repeat region
LRR_14 PF23598 273 - 398 3.4e-06 Leucine-rich repeat region
LRR_8 PF13855 366 - 424 5.9e-07 Leucine rich repeat
LRR_14 PF23598 384 - 519 4.3e-08 Leucine-rich repeat region
LRR_8 PF13855 437 - 496 4.1e-11 Leucine rich repeat
LRR_8 PF13855 480 - 520 3.3e-06 Leucine rich repeat
Pkinase PF00069 660 - 920 5.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 662 - 922 1.4e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2440
Acc36I ACCTGC 1 cut(s) 1777
AccB7I CCANNNNNTGG 2 cut(s) 748, 1007
AccI GTMKAC 3 cut(s) 2444, 2574, 2643
AccII CGCG 2 cut(s) 2491, 2763
AccIII TCCGGA 1 cut(s) 2171
AciI CCGC 5 cut(s) 1214, 2500, 2722, 2790, 2822
AclI AACGTT 1 cut(s) 1110
AclWI GGATC 5 cut(s) 343, 759, 997, 1903, 2226
AcoI YGGCCR 5 cut(s) 343, 1835, 2046, 2738, 2769
AcsI RAATTY 5 cut(s) 940, 1396, 1531, 1921, 2090
AcuI CTGAAG 3 cut(s) 545, 1208, 1576
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 7 cut(s) 1162, 1605, 1940, 2008, 2015, 2156, 2873
AfiI CCNNNNNNNGG 8 cut(s) 352, 748, 874, 935, 1007, 1079, 1417, 2680
AflIII ACRYGT 1 cut(s) 215
AgeI ACCGGT 3 cut(s) 701, 1879, 2680
AhdI GACNNNNNGTC 1 cut(s) 2265
AjnI CCWGG 6 cut(s) 387, 741, 1429, 1626, 1776, 2148
AjuI GAANNNNNNNTTGG 2 cut(s) 1840, 1872
AleI CACNNNNGTG 1 cut(s) 2849
AloI GAACNNNNNNTCC 2 cut(s) 625, 657
Alw21I GWGCWC 4 cut(s) 1512, 1551, 2717, 2921
Alw26I GTCTC 6 cut(s) 411, 1740, 2090, 2216, 2273, 2780
Alw44I GTGCAC 1 cut(s) 2713
AlwI GGATC 5 cut(s) 343, 759, 997, 1903, 2226
AlwNI CAGNNNCTG 2 cut(s) 1231, 2214
Ama87I CYCGRG 2 cut(s) 1503, 2648
Aor13HI TCCGGA 1 cut(s) 2171
ApaI GGGCCC 1 cut(s) 1798
ApaLI GTGCAC 1 cut(s) 2713
ApeKI GCWGC 4 cut(s) 900, 2288, 2322, 2725
ApoI RAATTY 5 cut(s) 940, 1396, 1531, 1921, 2090
ArsI GACNNNNNNTTYG 2 cut(s) 2363, 2395
AseI ATTAAT 1 cut(s) 1496
AsiGI ACCGGT 3 cut(s) 701, 1879, 2680
Asp700I GAANNNNTTC 3 cut(s) 637, 1383, 2693
AspA2I CCTAGG 1 cut(s) 934
AspLEI GCGC 1 cut(s) 2491
AsuC2I CCSGG 3 cut(s) 353, 1564, 2342
AsuHPI GGTGA 8 cut(s) 171, 377, 1662, 1918, 2152, 2213, 2695, 2836
AsuII TTCGAA 1 cut(s) 2382
AvaI CYCGRG 2 cut(s) 1503, 2648
AvaII GGWCC 5 cut(s) 488, 917, 1133, 1277, 2678
AvrII CCTAGG 1 cut(s) 934
BaeGI GKGCMC 3 cut(s) 1798, 2462, 2717
BalI TGGCCA 2 cut(s) 2048, 2771
BanII GRGCYC 2 cut(s) 1512, 1798
BauI CACGAG 2 cut(s) 1156, 1645
BbsI GAAGAC 1 cut(s) 2686
Bbv12I GWGCWC 4 cut(s) 1512, 1551, 2717, 2921
BbvI GCAGC 4 cut(s) 912, 2300, 2334, 2737
BccI CCATC 9 cut(s) 5, 862, 1059, 1217, 1856, 2057, 2601, 2833, 2917
BceAI ACGGC 2 cut(s) 2555, 2839
BcgI CGANNNNNNTGC 2 cut(s) 2656, 2690
BciT130I CCWGG 6 cut(s) 389, 743, 1431, 1628, 1778, 2150
BciVI GTATCC 1 cut(s) 1309
BcnI CCSGG 3 cut(s) 353, 1564, 2342
BcoDI GTCTC 6 cut(s) 411, 1740, 2090, 2216, 2273, 2780
BfaI CTAG 2 cut(s) 539, 935
BfmI CTRYAG 3 cut(s) 308, 596, 1520
BfuAI ACCTGC 1 cut(s) 1777
BfuI GTATCC 1 cut(s) 1309
BglII AGATCT 2 cut(s) 295, 2652
BisI GCNGC 5 cut(s) 901, 2289, 2323, 2723, 2726
BlnI CCTAGG 1 cut(s) 934
BlsI GCNGC 5 cut(s) 902, 2290, 2324, 2724, 2727
Bme1390I CCNGG 9 cut(s) 353, 389, 743, 1431, 1564, 1628, 1778, 2150, 2342
Bme18I GGWCC 5 cut(s) 488, 917, 1133, 1277, 2678
BmeRI GACNNNNNGTC 1 cut(s) 2265
BmeT110I CYCGRG 2 cut(s) 1503, 2648
BmiI GGNNCC 4 cut(s) 1207, 1351, 1795, 1796
BmrFI CCNGG 9 cut(s) 353, 389, 743, 1431, 1564, 1628, 1778, 2150, 2342
BmrI ACTGGG 2 cut(s) 672, 2062
BmsI GCATC 6 cut(s) 861, 2009, 2130, 2137, 2320, 2917
BmuI ACTGGG 2 cut(s) 672, 2062
BoxI GACNNNNGTC 1 cut(s) 1015
BpiI GAAGAC 1 cut(s) 2686
BpmI CTGGAG 6 cut(s) 627, 795, 1253, 2132, 2255, 2567
Bpu14I TTCGAA 1 cut(s) 2382
BpuEI CTTGAG 3 cut(s) 1645, 1990, 2927
BpuMI CCSGG 3 cut(s) 353, 1564, 2342
BsaAI YACGTR 2 cut(s) 2863, 2875
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 210
BsaWI WCCGGW 5 cut(s) 701, 1072, 1879, 2171, 2680
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 8 cut(s) 352, 748, 874, 935, 1007, 1079, 1417, 2680
Bse118I RCCGGY 5 cut(s) 701, 1833, 1879, 2680, 2736
Bse1I ACTGG 8 cut(s) 587, 655, 667, 778, 2042, 2068, 2261, 2772
Bse3DI GCAATG 3 cut(s) 237, 2406, 2629
Bse8I GATNNNNATC 1 cut(s) 1050
BseAI TCCGGA 1 cut(s) 2171
BseBI CCWGG 6 cut(s) 389, 743, 1431, 1628, 1778, 2150
BseGI GGATG 7 cut(s) 151, 361, 854, 1209, 1573, 1962, 2350
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 8 cut(s) 352, 748, 874, 935, 1007, 1079, 1417, 2680
BseMI GCAATG 3 cut(s) 237, 2406, 2629
BseMII CTCAG 7 cut(s) 568, 1165, 1525, 2472, 2499, 2937, 2978
BseNI ACTGG 8 cut(s) 587, 655, 667, 778, 2042, 2068, 2261, 2772
BseRI GAGGAG 1 cut(s) 2158
BseSI GKGCMC 3 cut(s) 1798, 2462, 2717
BseXI GCAGC 4 cut(s) 912, 2300, 2334, 2737
BseYI CCCAGC 2 cut(s) 121, 2931
BsgI GTGCAG 1 cut(s) 715
Bsh1236I CGCG 2 cut(s) 2491, 2763
BshTI ACCGGT 3 cut(s) 701, 1879, 2680
BsiHKAI GWGCWC 4 cut(s) 1512, 1551, 2717, 2921
BsiHKCI CYCGRG 2 cut(s) 1503, 2648
BslFI GGGAC 2 cut(s) 104, 493
BslI CCNNNNNNNGG 8 cut(s) 352, 748, 874, 935, 1007, 1079, 1417, 2680
BsmAI GTCTC 6 cut(s) 411, 1740, 2090, 2216, 2273, 2780
BsmBI CGTCTC 1 cut(s) 2273
BsmFI GGGAC 2 cut(s) 104, 493
BsmI GAATGC 1 cut(s) 708
BsoBI CYCGRG 2 cut(s) 1503, 2648
Bsp119I TTCGAA 1 cut(s) 2382
Bsp120I GGGCCC 1 cut(s) 1794
Bsp1286I GDGCHC 6 cut(s) 1512, 1551, 1798, 2462, 2717, 2921
Bsp13I TCCGGA 1 cut(s) 2171
Bsp1407I TGTACA 2 cut(s) 1160, 2013
BspACI CCGC 5 cut(s) 1214, 2500, 2722, 2790, 2822
BspCNI CTCAG 7 cut(s) 567, 1164, 1524, 2473, 2500, 2936, 2977
BspEI TCCGGA 1 cut(s) 2171
BspFNI CGCG 2 cut(s) 2491, 2763
BspHI TCATGA 4 cut(s) 1729, 1812, 1950, 2314
BspLI GGNNCC 4 cut(s) 1207, 1351, 1795, 1796
BspMAI CTGCAG 1 cut(s) 312
BspMI ACCTGC 1 cut(s) 1777
BspPI GGATC 5 cut(s) 343, 759, 997, 1903, 2226
BspQI GCTCTTC 1 cut(s) 1227
BspT104I TTCGAA 1 cut(s) 2382
BsrDI GCAATG 3 cut(s) 237, 2406, 2629
BsrFI RCCGGY 5 cut(s) 701, 1833, 1879, 2680, 2736
BsrGI TGTACA 2 cut(s) 1160, 2013
BsrI ACTGG 8 cut(s) 587, 655, 667, 778, 2042, 2068, 2261, 2772
BssAI RCCGGY 5 cut(s) 701, 1833, 1879, 2680, 2736
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 2 cut(s) 1156, 1645
BssT1I CCWWGG 6 cut(s) 569, 934, 1320, 1709, 2281, 2293
Bst2BI CACGAG 2 cut(s) 1156, 1645
Bst2UI CCWGG 6 cut(s) 389, 743, 1431, 1628, 1778, 2150
Bst6I CTCTTC 1 cut(s) 1227
BstACI GRCGYC 1 cut(s) 210
BstAUI TGTACA 2 cut(s) 1160, 2013
BstBAI YACGTR 2 cut(s) 2863, 2875
BstBI TTCGAA 1 cut(s) 2382
BstDEI CTNAG 7 cut(s) 554, 1151, 1511, 2481, 2508, 2923, 2964
BstENI CCTNNNNNAGG 2 cut(s) 872, 933
BstF5I GGATG 7 cut(s) 151, 361, 854, 1209, 1573, 1962, 2350
BstFNI CGCG 2 cut(s) 2491, 2763
BstHHI GCGC 1 cut(s) 2491
BstMAI GTCTC 6 cut(s) 411, 1740, 2090, 2216, 2273, 2780
BstMWI GCNNNNNNNGC 8 cut(s) 26, 1516, 1793, 2285, 2466, 2551, 2746, 2896
BstNI CCWGG 6 cut(s) 389, 743, 1431, 1628, 1778, 2150
BstNSI RCATGY 4 cut(s) 219, 974, 2667, 2899
BstPAI GACNNNNGTC 1 cut(s) 1015
BstSCI CCNGG 9 cut(s) 351, 387, 741, 1429, 1562, 1626, 1776, 2148, 2340
BstSFI CTRYAG 3 cut(s) 308, 596, 1520
BstSLI GKGCMC 3 cut(s) 1798, 2462, 2717
BstUI CGCG 2 cut(s) 2491, 2763
BstV1I GCAGC 4 cut(s) 912, 2300, 2334, 2737
BstV2I GAAGAC 1 cut(s) 2686
BstX2I RGATCY 4 cut(s) 295, 348, 751, 2652
BstXI CCANNNNNNTGG 2 cut(s) 662, 773
BstYI RGATCY 4 cut(s) 295, 348, 751, 2652
BsuI GTATCC 1 cut(s) 1309
BtsCI GGATG 7 cut(s) 151, 361, 854, 1209, 1573, 1962, 2350
BtsI GCAGTG 3 cut(s) 116, 377, 732
BtsIMutI CAGTG 9 cut(s) 116, 377, 562, 732, 1577, 2016, 2049, 2505, 2977
BveI ACCTGC 1 cut(s) 1777
CaiI CAGNNNCTG 2 cut(s) 1231, 2214
CciI TCATGA 4 cut(s) 1729, 1812, 1950, 2314
CfoI GCGC 1 cut(s) 2491
Cfr10I RCCGGY 5 cut(s) 701, 1833, 1879, 2680, 2736
CpoI CGGWCCG 1 cut(s) 2678
CseI GACGC 1 cut(s) 199
Csp6I GTAC 7 cut(s) 1161, 1604, 1939, 2007, 2014, 2155, 2872
CspAI ACCGGT 3 cut(s) 701, 1879, 2680
CspCI CAANNNNNGTGG 4 cut(s) 1183, 1218, 1327, 1362
CspI CGGWCCG 1 cut(s) 2678
CviQI GTAC 7 cut(s) 1161, 1604, 1939, 2007, 2014, 2155, 2872
DdeI CTNAG 7 cut(s) 554, 1151, 1511, 2481, 2508, 2923, 2964
DraI TTTAAA 1 cut(s) 468
DrdI GACNNNNNNGTC 1 cut(s) 2440
DriI GACNNNNNGTC 1 cut(s) 2265
DseDI GACNNNNNNGTC 1 cut(s) 2440
EaeI YGGCCR 5 cut(s) 343, 1835, 2046, 2738, 2769
Eam1104I CTCTTC 1 cut(s) 1227
Eam1105I GACNNNNNGTC 1 cut(s) 2265
EarI CTCTTC 1 cut(s) 1227
Ecl136II GAGCTC 1 cut(s) 1510
Eco130I CCWWGG 6 cut(s) 569, 934, 1320, 1709, 2281, 2293
Eco24I GRGCYC 2 cut(s) 1512, 1798
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 5 cut(s) 488, 917, 1133, 1277, 2678
Eco53kI GAGCTC 1 cut(s) 1510
Eco57I CTGAAG 3 cut(s) 545, 1208, 1576
Eco88I CYCGRG 2 cut(s) 1503, 2648
EcoICRI GAGCTC 1 cut(s) 1510
EcoNI CCTNNNNNAGG 2 cut(s) 872, 933
EcoO109I RGGNCCY 3 cut(s) 1133, 1794, 1795
EcoRII CCWGG 6 cut(s) 387, 741, 1429, 1626, 1776, 2148
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 6 cut(s) 569, 934, 1320, 1709, 2281, 2293
EcoT38I GRGCYC 2 cut(s) 1512, 1798
ErhI CCWWGG 6 cut(s) 569, 934, 1320, 1709, 2281, 2293
Esp3I CGTCTC 1 cut(s) 2273
FalI AAGNNNNNCTT 2 cut(s) 2678, 2710
FaqI GGGAC 2 cut(s) 104, 493
FauI CCCGC 4 cut(s) 1221, 2507, 2797, 2829
FblI GTMKAC 3 cut(s) 2444, 2574, 2643
Fnu4HI GCNGC 5 cut(s) 901, 2289, 2323, 2723, 2726
FokI GGATG 7 cut(s) 158, 368, 841, 1196, 1580, 1969, 2357
FriOI GRGCYC 2 cut(s) 1512, 1798
Fsp4HI GCNGC 5 cut(s) 901, 2289, 2323, 2723, 2726
FspBI CTAG 2 cut(s) 539, 935
GlaI GCGC 1 cut(s) 2490
GluI GCNGC 5 cut(s) 901, 2289, 2323, 2723, 2726
GsaI CCCAGC 2 cut(s) 125, 2935
GsuI CTGGAG 6 cut(s) 627, 795, 1253, 2132, 2255, 2567
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 2491
Hin1I GRCGYC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 2489
HinP1I GCGC 1 cut(s) 2489
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 2 cut(s) 980, 1022
HpaI GTTAAC 1 cut(s) 660
HphI GGTGA 8 cut(s) 171, 377, 1662, 1918, 2152, 2213, 2695, 2836
Hpy99I CGWCG 1 cut(s) 1640
HpyCH4IV ACGT 5 cut(s) 1110, 1638, 2441, 2862, 2874
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 1516, 1793, 2285, 2466, 2551, 2746, 2896
HpyF3I CTNAG 7 cut(s) 554, 1151, 1511, 2481, 2508, 2923, 2964
HpySE526I ACGT 5 cut(s) 1110, 1638, 2441, 2862, 2874
Hsp92I GRCGYC 1 cut(s) 210
HspAI GCGC 1 cut(s) 2489
Kpn2I TCCGGA 1 cut(s) 2171
KroI GCCGGC 1 cut(s) 1833
KroNI GCCGGC 1 cut(s) 1835
KspAI GTTAAC 1 cut(s) 660
LguI GCTCTTC 1 cut(s) 1227
LmnI GCTCC 2 cut(s) 2285, 2841
Lsp1109I GCAGC 4 cut(s) 912, 2300, 2334, 2737
LweI GCATC 6 cut(s) 861, 2009, 2130, 2137, 2320, 2917
MaeI CTAG 2 cut(s) 539, 935
MaeII ACGT 5 cut(s) 1110, 1638, 2441, 2862, 2874
MaeIII GTNAC 6 cut(s) 211, 365, 1307, 1991, 2755, 2829
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 4 cut(s) 295, 348, 751, 2652
MhlI GDGCHC 6 cut(s) 1512, 1551, 1798, 2462, 2717, 2921
MlsI TGGCCA 2 cut(s) 2048, 2771
MluNI TGGCCA 2 cut(s) 2048, 2771
MlyI GAGTC 3 cut(s) 86, 1411, 1742
MmeI TCCRAC 5 cut(s) 610, 1287, 1766, 1871, 2095
Mox20I TGGCCA 2 cut(s) 2048, 2771
MroI TCCGGA 1 cut(s) 2171
MroNI GCCGGC 1 cut(s) 1833
MroXI GAANNNNTTC 3 cut(s) 637, 1383, 2693
MscI TGGCCA 2 cut(s) 2048, 2771
MslI CAYNNNNRTG 5 cut(s) 1955, 2226, 2343, 2394, 2849
Msp20I TGGCCA 2 cut(s) 2048, 2771
MspA1I CMGCKG 1 cut(s) 2935
MspR9I CCNGG 9 cut(s) 353, 389, 743, 1431, 1564, 1628, 1778, 2150, 2342
MunI CAATTG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 6 cut(s) 389, 743, 1431, 1628, 1778, 2150
MvnI CGCG 2 cut(s) 2491, 2763
MwoI GCNNNNNNNGC 8 cut(s) 26, 1516, 1793, 2285, 2466, 2551, 2746, 2896
NaeI GCCGGC 1 cut(s) 1835
NciI CCSGG 3 cut(s) 353, 1564, 2342
NgoMIV GCCGGC 1 cut(s) 1833
NlaIV GGNNCC 4 cut(s) 1207, 1351, 1795, 1796
NmuCI GTSAC 3 cut(s) 211, 365, 2829
NspI RCATGY 4 cut(s) 219, 974, 2667, 2899
NspV TTCGAA 1 cut(s) 2382
OliI CACNNNNGTG 1 cut(s) 2849
PaeI GCATGC 1 cut(s) 974
PaeR7I CTCGAG 1 cut(s) 1503
PagI TCATGA 4 cut(s) 1729, 1812, 1950, 2314
PciI ACATGT 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 1227
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 708
PdiI GCCGGC 1 cut(s) 1835
PdmI GAANNNNTTC 3 cut(s) 637, 1383, 2693
PflFI GACNNNGTC 1 cut(s) 817
PflMI CCANNNNNTGG 2 cut(s) 748, 1007
PfoI TCCNGGA 1 cut(s) 351
PinAI ACCGGT 3 cut(s) 701, 1879, 2680
PkrI GCNGC 5 cut(s) 902, 2290, 2324, 2724, 2727
PleI GAGTC 3 cut(s) 86, 1410, 1741
PpsI GAGTC 3 cut(s) 86, 1410, 1741
Ppu21I YACGTR 2 cut(s) 2863, 2875
PpuMI RGGWCCY 1 cut(s) 1133
PscI ACATGT 1 cut(s) 215
PshAI GACNNNNGTC 1 cut(s) 1015
PshBI ATTAAT 1 cut(s) 1496
Psp124BI GAGCTC 1 cut(s) 1512
Psp1406I AACGTT 1 cut(s) 1110
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 6 cut(s) 387, 741, 1429, 1626, 1776, 2148
PspFI CCCAGC 2 cut(s) 121, 2931
PspGI CCWGG 6 cut(s) 387, 741, 1429, 1626, 1776, 2148
PspN4I GGNNCC 4 cut(s) 1207, 1351, 1795, 1796
PspOMI GGGCCC 1 cut(s) 1794
PspPPI RGGWCCY 1 cut(s) 1133
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 2 cut(s) 1231, 2214
PsuI RGATCY 4 cut(s) 295, 348, 751, 2652
PsyI GACNNNGTC 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 2935
RsaI GTAC 7 cut(s) 1162, 1605, 1940, 2008, 2015, 2156, 2873
RsaNI GTAC 7 cut(s) 1161, 1604, 1939, 2007, 2014, 2155, 2872
RseI CAYNNNNRTG 5 cut(s) 1955, 2226, 2343, 2394, 2849
Rsr2I CGGWCCG 1 cut(s) 2678
RsrII CGGWCCG 1 cut(s) 2678
SacI GAGCTC 1 cut(s) 1512
SapI GCTCTTC 1 cut(s) 1227
SatI GCNGC 5 cut(s) 901, 2289, 2323, 2723, 2726
SchI GAGTC 3 cut(s) 86, 1411, 1742
ScrFI CCNGG 9 cut(s) 353, 389, 743, 1431, 1564, 1628, 1778, 2150, 2342
SduI GDGCHC 6 cut(s) 1512, 1551, 1798, 2462, 2717, 2921
SfaNI GCATC 6 cut(s) 861, 2009, 2130, 2137, 2320, 2917
SfcI CTRYAG 3 cut(s) 308, 596, 1520
Sfr274I CTCGAG 1 cut(s) 1503
SfuI TTCGAA 1 cut(s) 2382
SinI GGWCC 5 cut(s) 488, 917, 1133, 1277, 2678
SlaI CTCGAG 1 cut(s) 1503
SmiMI CAYNNNNRTG 5 cut(s) 1955, 2226, 2343, 2394, 2849
SmlI CTYRAG 4 cut(s) 1503, 1660, 1969, 2942
SmoI CTYRAG 4 cut(s) 1503, 1660, 1969, 2942
SphI GCATGC 1 cut(s) 974
SsiI CCGC 5 cut(s) 1214, 2500, 2722, 2790, 2822
SspI AATATT 1 cut(s) 2362
SspMI CTAG 2 cut(s) 539, 935
SstI GAGCTC 1 cut(s) 1512
StyD4I CCNGG 9 cut(s) 351, 387, 741, 1429, 1562, 1626, 1776, 2148, 2340
StyI CCWWGG 6 cut(s) 569, 934, 1320, 1709, 2281, 2293
TaiI ACGT 5 cut(s) 1113, 1641, 2444, 2865, 2877
TaqI TCGA 9 cut(s) 300, 431, 1290, 1405, 1504, 1716, 1723, 2253, 2382
TaqII GACCGA 1 cut(s) 1106
TatI WGTACW 4 cut(s) 1160, 1603, 2006, 2013
TauI GCSGC 1 cut(s) 2725
TscAI CASTG 9 cut(s) 123, 384, 562, 739, 1584, 2016, 2049, 2512, 2977
TseFI GTSAC 3 cut(s) 211, 365, 2829
TseI GCWGC 4 cut(s) 900, 2288, 2322, 2725
Tsp45I GTSAC 3 cut(s) 211, 365, 2829
TspGWI ACGGA 3 cut(s) 774, 847, 2630
TspRI CASTG 9 cut(s) 123, 384, 562, 739, 1584, 2016, 2049, 2512, 2977
Tth111I GACNNNGTC 1 cut(s) 817
Van91I CCANNNNNTGG 2 cut(s) 748, 1007
VneI GTGCAC 1 cut(s) 2713
VpaK11BI GGWCC 5 cut(s) 488, 917, 1133, 1277, 2678
VspI ATTAAT 1 cut(s) 1496
XagI CCTNNNNNAGG 2 cut(s) 872, 933
XapI RAATTY 5 cut(s) 940, 1396, 1531, 1921, 2090
XceI RCATGY 4 cut(s) 219, 974, 2667, 2899
XcmI CCANNNNNNNNNTGG 2 cut(s) 2279, 2365
XhoI CTCGAG 1 cut(s) 1503
XmaJI CCTAGG 1 cut(s) 934
XmiI GTMKAC 3 cut(s) 2444, 2574, 2643
XmnI GAANNNNTTC 3 cut(s) 637, 1383, 2693
XspI CTAG 2 cut(s) 539, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.