RLG00000010368
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016560
Physical Location & Seq
Reverse (-)
1032223 .. 1038927
6705 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010368

Sequence Viewer

Length: 2856 bp
ATGAAGAAAAAAGAGTTTTTAATATGGGGTTTTAATAGGAGGGAAGTTATGGCATTTCGGTTTGACTTGTTGCTGCTTCTGGGTTTGCTCGTTTGTGTGAGCTTTGGTAATGTGGATTCAGATGAAGGAGCAACACTGCTGGAGATAAAGAAGTCGTTTAGGGATGTGGACAATGTTCTATACGACTGGAGAGACTCACCGTCTTTAGACTACTGTGTTTGGAGAGGCGTCACATGTGACAATGTCACCTTCAATGTCATAGCACTCAATCTATCAGGTCTAAATCTTGATGGAGAAATCTCACCTGCAATAGGAAATCTCAAGGGCCTCCAGTCCATTGATTTGAGAGGGAACCGACTTTCAGGCCAGATCCCAGATGAGATTGGTGACTGTTCGTCTCTGAGATACCTGGACCTGTCCTTCAATGAGATATATGGAGACATCCCATTTTCAATATCCAAGTTGAAGCAACTGGAAAGCATAATGTTGAAAAATAATCAGTTGATTGGACCACTCCCTTCAACACTGTCCCAGATTCCGAACCTGAAGATGTTTGATGTGCGAAACAACAGTTTGACTGGTAGTATTCCTCAAAATATAGGGAACTGCACTGCCTTCCAAGTCTTGGACCTGTCCTATAACCAGCTAACTGGAGAGATTCCTTTCAACATTGGGTTCCTGCAAGTAGCCACCTTATCATTGCAGGGTAATCAACTTTCTGGGCCTATCCCTTCCGTTATTGGCCTGATGCAGGCACTTGCTGTATTAGATTTGAGTGGCAACATGCTAAGTGGACCGATTCCTCCTATTCTGGGGAATTTGACTTATACAGAGAAATTGTATTTGCATGCCAACAAGCTTACAGGATCAATTCCCCCAGAGCTCGGACAAATGGCACAGCTTCATTATTTGGTGTTGAATGATAACCATCTTACTGGACAAATTCCACCTGAACTTGGGAAGCTTACCAACTTGTTTGACCTAAATGTTGCTAACAACAATCTTCAAGGGCCCATCCCTGATAATATTAGCTCATGTACAAATCTCAACACCCTCAATGTGCATGGGAACAGATTGAATGGAACCATTCCGCCTGCTCTCCAGAGGCTGGAGAGTATGACTTATCTAAATCTGTCCTCCAACAATCTTCGTGGCTCAATACCAATTGAGCTATCTCGGATTGGCAATTTGGATACTTTGGATCTTTCTAATAACAAGTTCAGTGGATCCATACCTTCTTCGCTTGGTGATTTGGAACATCTTCTTAAGCTGAATTTGAGTAGAAATCATATGACAGGATTTATTCCAGCAGAGTTTGGTAATCTAAGGAGTGTTGTGGACATAGATCTTTCAAGTAATCGGCTCACAGGAATGATTCCTCAGGAGCTCAGTCAGCTGCAGAATTTGTTCTCACTGAGGCTAGAACATAACAATATATCAGGGGAAGTGGTGTCTTTGATCAATTGCCTCAGTCTTGTAGTGCTAAATGTGTCTTATAACAACCTGGCGGGAGATATTCCCACATCCAATAACTTCTCAAGGTTTTCACCCGACAGCTTTGTTGGAAATCCTGATCTTTGTGGCTATTGGCTCAATTCTCCGTGTCATGAGTCCCGTCCAACCGAGAGAGTTTCATTATCTAAAGCGGCTATCCTGGGAATTGCTCTTGGTGCCCTTGTGATTCTTCTCATGATCCTTATTGCCGCCTGCCGCCCTTACCATCCAACTCCTTTTCCTGACGGTACACTTGACAAACCAGTTAATTACTCAACACCAAAGCTAGTGATTCTTAACATGAATATGGCACTTCATGTGTATGAGGATATCATGAGGATGACTGAAAACTTGAGTGAGAAGTATATAATTGGTTATGGCGCATCAAGCACGGTATACAAATGTGTTCTTAAGAATTGTAAGCCAGTGGCGATCAAGAGACTTTACTCTCATTATCCACAGTGCTTGAAGGAATTTGAGACAGAACTTGGAACAGTTGGCAGCATCAAGCATCGAAATCTAGTCAGCCTCCAAGGATACTCCTTGTCTTCTTCTGGAAACCTTCTCTTTTATGATTACATGGAAAATGGCAGTCTCTGGGATCTCCTTCATGGCCCAACTACCAAGAAGAAGAAGCTTGACTGGGAAACTCGTATCCAGATAGCACTCGGAGCAGCCCAAGGGCTTGCCTATCTCCATCATGATTGTAGTCCACGTATCATACACAGGGATGTGAAGTCGTCTAACATTCTGTTGGACAAGGATTTCGAGGCTCATTTAACTGATTTTGGTATTGCCAAGAGCTTATGCACGTCAAAGACCCATACATCTACTTGTGTAATGGGCACCATTGGCTACATAGACCCTGAGTATGCTCGAACTTCCCGCCTCACTGAGAAGTCGGATGTTTATAGTTATGGGATTGTCCTGCTGGAGTTGCTAACAGGAAGGAAGGCTGTAGACAATGAATGCAATCTCCATCAATTGATCTTATCAAAGACCGCAAACAATGCTGTGATGGAAACTGTTGATCCAGAGATCATGGCCACATGCATGGACCTTGGAGCAGTGAAGAAGGTTTTTCAGCTTGCACTCCTATGCACAAAGAGGCAGCCAACAGACCGGCCAACTATGCATGAAATAACACGAGTTTTGGGTAGCCTTGTGCCATCACCTACATTGCCGAAACAAGCAACCCTCAATCCTCCATCCACTACACTCTCATCTGTCAAAGCGCCGTGCTACATGGATGAGTATGCAAACCTCAAAACCCCACACATGCTCAATTGCCCATCTATGAGTACCTCAGACGCTCAGCTCTTTCTCAAGTTTGGAGAGGTAATTTCTCAGAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

952

Amino Acids

104.89

Weight (kDa)

6.05

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 80 6.7e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 104 - 144 4.5e-06 Leucine rich repeat
LRR_14 PF23598 127 - 211 4.3e-07 Leucine-rich repeat region
LRR_8 PF13855 347 - 407 1.5e-07 Leucine rich repeat
LRR_14 PF23598 367 - 479 5.8e-09 Leucine-rich repeat region
LRR_8 PF13855 396 - 455 3.9e-09 Leucine rich repeat
LRR_8 PF13855 420 - 479 2.2e-08 Leucine rich repeat
Pkinase PF00069 619 - 880 2.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 621 - 882 6.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1497
AarI CACCTGC 1 cut(s) 313
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 2 cut(s) 1670, 2339
AccB7I CCANNNNNTGG 2 cut(s) 625, 1108
AccI GTMKAC 2 cut(s) 1890, 2454
AciI CCGC 7 cut(s) 1091, 1508, 1646, 1704, 1711, 2380, 2496
AclWI GGATC 8 cut(s) 364, 874, 1209, 1221, 1234, 1687, 2103, 2519
AcoI YGGCCR 2 cut(s) 2538, 2618
AcsI RAATTY 5 cut(s) 817, 942, 1273, 1402, 1967
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 3 cut(s) 1039, 1744, 2797
AfiI CCNNNNNNNGG 7 cut(s) 311, 625, 751, 812, 884, 956, 1108
AflII CTTAAG 2 cut(s) 1265, 1904
AflIII ACRYGT 1 cut(s) 233
AhdI GACNNNNNGTC 2 cut(s) 199, 394
AjiI CACGTC 1 cut(s) 2307
AjnI CCWGG 3 cut(s) 408, 1503, 1653
Alw21I GWGCWC 2 cut(s) 885, 1389
Alw26I GTCTC 6 cut(s) 186, 402, 432, 1927, 1967, 2093
AlwI GGATC 8 cut(s) 364, 874, 1209, 1221, 1234, 1687, 2103, 2519
AlwNI CAGNNNCTG 2 cut(s) 1108, 2091
AoxI GGCC 8 cut(s) 325, 364, 722, 742, 1010, 2107, 2538, 2618
ApaI GGGCCC 1 cut(s) 1014
ApeKI GCWGC 5 cut(s) 73, 1396, 1995, 2168, 2605
ApoI RAATTY 5 cut(s) 817, 942, 1273, 1402, 1967
ArsI GACNNNNNNTTYG 2 cut(s) 2243, 2275
Asp700I GAANNNNTTC 2 cut(s) 1260, 1406
AspLEI GCGC 2 cut(s) 1877, 2731
AsuHPI GGTGA 7 cut(s) 189, 238, 294, 398, 1259, 1539, 2658
AvaII GGWCC 5 cut(s) 412, 509, 628, 794, 2551
AxyI CCTNAGG 1 cut(s) 1380
BaeGI GKGCMC 3 cut(s) 1014, 1675, 2342
BalI TGGCCA 1 cut(s) 2540
BamHI GGATCC 1 cut(s) 1226
BanI GGYRCC 2 cut(s) 1670, 2339
BanII GRGCYC 3 cut(s) 885, 1014, 1389
BauI CACGAG 1 cut(s) 2640
BbsI GAAGAC 1 cut(s) 2034
Bbv12I GWGCWC 2 cut(s) 885, 1389
BbvI GCAGC 5 cut(s) 60, 1383, 2007, 2180, 2617
BceAI ACGGC 1 cut(s) 2716
BciT130I CCWGG 3 cut(s) 410, 1505, 1655
BciVI GTATCC 3 cut(s) 1186, 2024, 2159
BclI TGATCA 1 cut(s) 1458
BcoDI GTCTC 6 cut(s) 186, 402, 432, 1927, 1967, 2093
BfaI CTAG 3 cut(s) 1421, 1781, 2015
BfmI CTRYAG 2 cut(s) 1397, 2451
BfoI RGCGCY 1 cut(s) 2732
BfrI CTTAAG 2 cut(s) 1265, 1904
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 3 cut(s) 1186, 2024, 2159
BglII AGATCT 1 cut(s) 1345
BisI GCNGC 8 cut(s) 74, 1397, 1647, 1704, 1711, 1996, 2169, 2606
BlpI GCTNAGC 1 cut(s) 2808
BlsI GCNGC 8 cut(s) 75, 1398, 1648, 1705, 1712, 1997, 2170, 2607
Bme1390I CCNGG 3 cut(s) 410, 1505, 1655
Bme18I GGWCC 5 cut(s) 412, 509, 628, 794, 2551
BmeRI GACNNNNNGTC 2 cut(s) 199, 394
BmgBI CACGTC 1 cut(s) 2307
BmiI GGNNCC 7 cut(s) 353, 677, 1012, 1084, 1228, 1672, 2341
BmrFI CCNGG 3 cut(s) 410, 1505, 1655
BmrI ACTGGG 1 cut(s) 2146
BmsI GCATC 4 cut(s) 738, 1886, 2007, 2014
BmuI ACTGGG 1 cut(s) 2146
BpiI GAAGAC 1 cut(s) 2034
BpmI CTGGAG 7 cut(s) 161, 208, 314, 672, 1085, 1130, 2447
Bpu1102I GCTNAGC 1 cut(s) 2808
BpuEI CTTGAG 4 cut(s) 305, 1522, 1867, 2804
BsaAI YACGTR 1 cut(s) 2210
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 4 cut(s) 1654, 2026, 2173, 2554
Bsc4I CCNNNNNNNGG 7 cut(s) 311, 625, 751, 812, 884, 956, 1108
Bse118I RCCGGY 1 cut(s) 2616
Bse1I ACTGG 9 cut(s) 191, 331, 477, 583, 655, 940, 1757, 1919, 2141
Bse21I CCTNAGG 1 cut(s) 1380
Bse3DI GCAATG 2 cut(s) 698, 2672
Bse8I GATNNNNATC 1 cut(s) 927
BseBI CCWGG 3 cut(s) 410, 1505, 1655
BseDI CCNNGG 4 cut(s) 1654, 2026, 2173, 2554
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 7 cut(s) 311, 625, 751, 812, 884, 956, 1108
BseMI GCAATG 2 cut(s) 698, 2672
BseNI ACTGG 9 cut(s) 191, 331, 477, 583, 655, 940, 1757, 1919, 2141
BseSI GKGCMC 3 cut(s) 1014, 1675, 2342
BseXI GCAGC 5 cut(s) 60, 1383, 2007, 2180, 2617
BsgI GTGCAG 1 cut(s) 592
BshFI GGCC 8 cut(s) 327, 366, 724, 744, 1012, 2109, 2540, 2620
BshNI GGYRCC 2 cut(s) 1670, 2339
BsiHKAI GWGCWC 2 cut(s) 885, 1389
BsiSI CCGG 1 cut(s) 2617
BslFI GGGAC 2 cut(s) 514, 1597
BslI CCNNNNNNNGG 7 cut(s) 311, 625, 751, 812, 884, 956, 1108
BsmAI GTCTC 6 cut(s) 186, 402, 432, 1927, 1967, 2093
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 514, 1597
BsmI GAATGC 1 cut(s) 2468
BsnI GGCC 8 cut(s) 327, 366, 724, 744, 1012, 2109, 2540, 2620
Bsp120I GGGCCC 1 cut(s) 1010
Bsp1286I GDGCHC 5 cut(s) 885, 1014, 1389, 1675, 2342
Bsp1407I TGTACA 1 cut(s) 1037
Bsp1720I GCTNAGC 1 cut(s) 2808
BspACI CCGC 7 cut(s) 1091, 1508, 1646, 1704, 1711, 2380, 2496
BspANI GGCC 8 cut(s) 327, 366, 724, 744, 1012, 2109, 2540, 2620
BspHI TCATGA 4 cut(s) 1606, 1689, 1827, 2194
BspLI GGNNCC 7 cut(s) 353, 677, 1012, 1084, 1228, 1672, 2341
BspMAI CTGCAG 1 cut(s) 1401
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 8 cut(s) 364, 874, 1209, 1221, 1234, 1687, 2103, 2519
BspT107I GGYRCC 2 cut(s) 1670, 2339
BspTI CTTAAG 2 cut(s) 1265, 1904
BsrDI GCAATG 2 cut(s) 698, 2672
BsrFI RCCGGY 1 cut(s) 2616
BsrGI TGTACA 1 cut(s) 1037
BsrI ACTGG 9 cut(s) 191, 331, 477, 583, 655, 940, 1757, 1919, 2141
BssAI RCCGGY 1 cut(s) 2616
BssECI CCNNGG 4 cut(s) 1654, 2026, 2173, 2554
BssNAI GTATAC 1 cut(s) 1891
BssNI GRCGYC 1 cut(s) 228
BssSI CACGAG 1 cut(s) 2640
BssT1I CCWWGG 3 cut(s) 2026, 2173, 2554
Bst1107I GTATAC 1 cut(s) 1891
Bst2BI CACGAG 1 cut(s) 2640
Bst2UI CCWGG 3 cut(s) 410, 1505, 1655
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 2 cut(s) 1265, 1904
BstAPI GCANNNNNTGC 1 cut(s) 2504
BstAUI TGTACA 1 cut(s) 1037
BstBAI YACGTR 1 cut(s) 2210
BstC8I GCNNGC 6 cut(s) 753, 849, 1095, 1708, 2181, 2583
BstENI CCTNNNNNAGG 2 cut(s) 309, 749
BstH2I RGCGCY 1 cut(s) 2732
BstHHI GCGC 2 cut(s) 1877, 2731
BstMAI GTCTC 6 cut(s) 186, 402, 432, 1927, 1967, 2093
BstMWI GCNNNNNNNGC 8 cut(s) 1393, 1670, 1881, 2165, 2346, 2431, 2504, 2626
BstNI CCWGG 3 cut(s) 410, 1505, 1655
BstNSI RCATGY 5 cut(s) 237, 787, 851, 2547, 2776
BstSCI CCNGG 3 cut(s) 408, 1503, 1653
BstSFI CTRYAG 2 cut(s) 1397, 2451
BstSLI GKGCMC 3 cut(s) 1014, 1675, 2342
BstV1I GCAGC 5 cut(s) 60, 1383, 2007, 2180, 2617
BstV2I GAAGAC 1 cut(s) 2034
BstX2I RGATCY 5 cut(s) 369, 1201, 1226, 1345, 2095
BstXI CCANNNNNNTGG 3 cut(s) 650, 935, 2548
BstYI RGATCY 5 cut(s) 369, 1201, 1226, 1345, 2095
BstZ17I GTATAC 1 cut(s) 1891
Bsu36I CCTNAGG 1 cut(s) 1380
BsuI GTATCC 3 cut(s) 1186, 2024, 2159
BsuRI GGCC 8 cut(s) 327, 366, 724, 744, 1012, 2109, 2540, 2620
BtrI CACGTC 1 cut(s) 2307
BtsI GCAGTG 3 cut(s) 134, 609, 2568
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 6 cut(s) 753, 849, 1095, 1708, 2181, 2583
CaiI CAGNNNCTG 2 cut(s) 1108, 2091
CciI TCATGA 4 cut(s) 1606, 1689, 1827, 2194
CfoI GCGC 2 cut(s) 1877, 2731
Cfr10I RCCGGY 1 cut(s) 2616
CseI GACGC 2 cut(s) 217, 2813
Csp6I GTAC 3 cut(s) 1038, 1743, 2796
CspCI CAANNNNNGTGG 6 cut(s) 1132, 1167, 1204, 1239, 1941, 1976
CviQI GTAC 3 cut(s) 1038, 1743, 2796
DriI GACNNNNNGTC 2 cut(s) 199, 394
EaeI YGGCCR 2 cut(s) 2538, 2618
Eam1105I GACNNNNNGTC 2 cut(s) 199, 394
EciI GGCGGA 1 cut(s) 1080
Ecl136II GAGCTC 2 cut(s) 883, 1387
Eco130I CCWWGG 3 cut(s) 2026, 2173, 2554
Eco24I GRGCYC 3 cut(s) 885, 1014, 1389
Eco32I GATATC 1 cut(s) 1825
Eco47I GGWCC 5 cut(s) 412, 509, 628, 794, 2551
Eco53kI GAGCTC 2 cut(s) 883, 1387
Eco57I CTGAAG 1 cut(s) 566
Eco81I CCTNAGG 1 cut(s) 1380
EcoICRI GAGCTC 2 cut(s) 883, 1387
EcoNI CCTNNNNNAGG 2 cut(s) 309, 749
EcoO109I RGGNCCY 2 cut(s) 325, 1010
EcoRII CCWGG 3 cut(s) 408, 1503, 1653
EcoRV GATATC 1 cut(s) 1825
EcoT14I CCWWGG 3 cut(s) 2026, 2173, 2554
EcoT22I ATGCAT 2 cut(s) 2549, 2631
EcoT38I GRGCYC 3 cut(s) 885, 1014, 1389
ErhI CCWWGG 3 cut(s) 2026, 2173, 2554
Esp3I CGTCTC 1 cut(s) 402
FaqI GGGAC 2 cut(s) 514, 1597
FauI CCCGC 2 cut(s) 1501, 2387
FauNDI CATATG 1 cut(s) 1290
FbaI TGATCA 1 cut(s) 1458
FblI GTMKAC 2 cut(s) 1890, 2454
Fnu4HI GCNGC 8 cut(s) 74, 1397, 1647, 1704, 1711, 1996, 2169, 2606
FriOI GRGCYC 3 cut(s) 885, 1014, 1389
Fsp4HI GCNGC 8 cut(s) 74, 1397, 1647, 1704, 1711, 1996, 2169, 2606
FspBI CTAG 3 cut(s) 1421, 1781, 2015
GlaI GCGC 2 cut(s) 1876, 2730
GluI GCNGC 8 cut(s) 74, 1397, 1647, 1704, 1711, 1996, 2169, 2606
GsuI CTGGAG 7 cut(s) 161, 208, 314, 672, 1085, 1130, 2447
HaeII RGCGCY 1 cut(s) 2732
HaeIII GGCC 8 cut(s) 327, 366, 724, 744, 1012, 2109, 2540, 2620
HapII CCGG 1 cut(s) 2617
HgaI GACGC 2 cut(s) 217, 2813
HhaI GCGC 2 cut(s) 1877, 2731
Hin1I GRCGYC 1 cut(s) 228
Hin6I GCGC 2 cut(s) 1875, 2729
HinP1I GCGC 2 cut(s) 1875, 2729
HindIII AAGCTT 3 cut(s) 857, 962, 2129
HinfI GANTC 9 cut(s) 116, 194, 535, 658, 799, 1375, 1610, 1681, 1786
HpaII CCGG 1 cut(s) 2617
HphI GGTGA 7 cut(s) 189, 238, 294, 398, 1259, 1539, 2658
Hpy166II GTNNAC 7 cut(s) 169, 794, 1339, 1745, 1891, 2207, 2455
Hpy188I TCNGA 9 cut(s) 121, 402, 540, 887, 1179, 2165, 2398, 2803, 2844
Hpy8I GTNNAC 7 cut(s) 169, 794, 1339, 1745, 1891, 2207, 2455
HpyCH4IV ACGT 2 cut(s) 2209, 2306
HpyF10VI GCNNNNNNNGC 8 cut(s) 1393, 1670, 1881, 2165, 2346, 2431, 2504, 2626
HpySE526I ACGT 2 cut(s) 2209, 2306
Hsp92I GRCGYC 1 cut(s) 228
HspAI GCGC 2 cut(s) 1875, 2729
Ksp22I TGATCA 1 cut(s) 1458
LmnI GCTCC 4 cut(s) 128, 1384, 2165, 2558
Lsp1109I GCAGC 5 cut(s) 60, 1383, 2007, 2180, 2617
LweI GCATC 4 cut(s) 738, 1886, 2007, 2014
MaeI CTAG 3 cut(s) 1421, 1781, 2015
MaeII ACGT 2 cut(s) 2209, 2306
MaeIII GTNAC 4 cut(s) 229, 236, 244, 386
MfeI CAATTG 4 cut(s) 1164, 1462, 2477, 2779
MflI RGATCY 5 cut(s) 369, 1201, 1226, 1345, 2095
MhlI GDGCHC 5 cut(s) 885, 1014, 1389, 1675, 2342
MlsI TGGCCA 1 cut(s) 2540
MluNI TGGCCA 1 cut(s) 2540
MlyI GAGTC 2 cut(s) 188, 1619
MmeI TCCRAC 6 cut(s) 1164, 1543, 1643, 1748, 2229, 2376
Mox20I TGGCCA 1 cut(s) 2540
Mph1103I ATGCAT 2 cut(s) 2549, 2631
MroXI GAANNNNTTC 2 cut(s) 1260, 1406
MscI TGGCCA 1 cut(s) 2540
MseI TTAA 7 cut(s) 20, 33, 1266, 1761, 1791, 1905, 2273
MslI CAYNNNNRTG 7 cut(s) 1370, 1799, 1815, 1832, 2223, 2546, 2590
Msp20I TGGCCA 1 cut(s) 2540
MspA1I CMGCKG 1 cut(s) 1396
MspCI CTTAAG 2 cut(s) 1265, 1904
MspI CCGG 1 cut(s) 2617
MspR9I CCNGG 3 cut(s) 410, 1505, 1655
MunI CAATTG 4 cut(s) 1164, 1462, 2477, 2779
Mva1269I GAATGC 1 cut(s) 2468
MvaI CCWGG 3 cut(s) 410, 1505, 1655
MwoI GCNNNNNNNGC 8 cut(s) 1393, 1670, 1881, 2165, 2346, 2431, 2504, 2626
NdeI CATATG 1 cut(s) 1290
NlaIV GGNNCC 7 cut(s) 353, 677, 1012, 1084, 1228, 1672, 2341
NmuCI GTSAC 4 cut(s) 229, 236, 244, 386
NsiI ATGCAT 2 cut(s) 2549, 2631
NspI RCATGY 5 cut(s) 237, 787, 851, 2547, 2776
PaeI GCATGC 1 cut(s) 851
PagI TCATGA 4 cut(s) 1606, 1689, 1827, 2194
PaqCI CACCTGC 1 cut(s) 313
PciI ACATGT 1 cut(s) 233
PctI GAATGC 1 cut(s) 2468
PdmI GAANNNNTTC 2 cut(s) 1260, 1406
PfeI GAWTC 7 cut(s) 116, 535, 658, 799, 1375, 1681, 1786
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 2 cut(s) 625, 1108
PkrI GCNGC 8 cut(s) 75, 1398, 1648, 1705, 1712, 1997, 2170, 2607
PleI GAGTC 2 cut(s) 188, 1618
PpsI GAGTC 2 cut(s) 188, 1618
Ppu21I YACGTR 1 cut(s) 2210
PscI ACATGT 1 cut(s) 233
PsiI TTATAA 1 cut(s) 1497
Psp124BI GAGCTC 2 cut(s) 885, 1389
Psp6I CCWGG 3 cut(s) 408, 1503, 1653
PspGI CCWGG 3 cut(s) 408, 1503, 1653
PspN4I GGNNCC 7 cut(s) 353, 677, 1012, 1084, 1228, 1672, 2341
PspOMI GGGCCC 1 cut(s) 1010
PstI CTGCAG 1 cut(s) 1401
PstNI CAGNNNCTG 2 cut(s) 1108, 2091
PsuI RGATCY 5 cut(s) 369, 1201, 1226, 1345, 2095
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 1396
RsaI GTAC 3 cut(s) 1039, 1744, 2797
RsaNI GTAC 3 cut(s) 1038, 1743, 2796
RseI CAYNNNNRTG 7 cut(s) 1370, 1799, 1815, 1832, 2223, 2546, 2590
SacI GAGCTC 2 cut(s) 885, 1389
SaqAI TTAA 7 cut(s) 20, 33, 1266, 1761, 1791, 1905, 2273
SatI GCNGC 8 cut(s) 74, 1397, 1647, 1704, 1711, 1996, 2169, 2606
SchI GAGTC 2 cut(s) 188, 1619
ScrFI CCNGG 3 cut(s) 410, 1505, 1655
SduI GDGCHC 5 cut(s) 885, 1014, 1389, 1675, 2342
SfaNI GCATC 4 cut(s) 738, 1886, 2007, 2014
SfcI CTRYAG 2 cut(s) 1397, 2451
SinI GGWCC 5 cut(s) 412, 509, 628, 794, 2551
SmiMI CAYNNNNRTG 7 cut(s) 1370, 1799, 1815, 1832, 2223, 2546, 2590
SmlI CTYRAG 6 cut(s) 320, 1265, 1537, 1846, 1904, 2819
SmoI CTYRAG 6 cut(s) 320, 1265, 1537, 1846, 1904, 2819
SphI GCATGC 1 cut(s) 851
SsiI CCGC 7 cut(s) 1091, 1508, 1646, 1704, 1711, 2380, 2496
SspI AATATT 1 cut(s) 1027
SspMI CTAG 3 cut(s) 1421, 1781, 2015
SstI GAGCTC 2 cut(s) 885, 1389
StyD4I CCNGG 3 cut(s) 408, 1503, 1653
StyI CCWWGG 3 cut(s) 2026, 2173, 2554
TaiI ACGT 2 cut(s) 2212, 2309
TaqI TCGA 3 cut(s) 2008, 2262, 2371
TaqII GACCGA 1 cut(s) 811
TatI WGTACW 1 cut(s) 1037
TauI GCSGC 3 cut(s) 1649, 1706, 1713
TfiI GAWTC 7 cut(s) 116, 535, 658, 799, 1375, 1681, 1786
Tru1I TTAA 7 cut(s) 20, 33, 1266, 1761, 1791, 1905, 2273
Tru9I TTAA 7 cut(s) 20, 33, 1266, 1761, 1791, 1905, 2273
TseFI GTSAC 4 cut(s) 229, 236, 244, 386
TseI GCWGC 5 cut(s) 73, 1396, 1995, 2168, 2605
Tsp45I GTSAC 4 cut(s) 229, 236, 244, 386
TspDTI ATGAA 9 cut(s) 17, 138, 893, 1623, 1799, 1811, 2093, 2475, 2646
TspGWI ACGGA 2 cut(s) 724, 1590
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 2 cut(s) 625, 1108
Vha464I CTTAAG 2 cut(s) 1265, 1904
VpaK11BI GGWCC 5 cut(s) 412, 509, 628, 794, 2551
XagI CCTNNNNNAGG 2 cut(s) 309, 749
XapI RAATTY 5 cut(s) 817, 942, 1273, 1402, 1967
XceI RCATGY 5 cut(s) 237, 787, 851, 2547, 2776
XmiI GTMKAC 2 cut(s) 1890, 2454
XmnI GAANNNNTTC 2 cut(s) 1260, 1406
XspI CTAG 3 cut(s) 1421, 1781, 2015
Zsp2I ATGCAT 2 cut(s) 2549, 2631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.