MD15G1049300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016560
Physical Location & Seq
Reverse (-)
3354019 .. 3361307
7289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1049300.v1.1.491

Sequence Viewer

Length: 2976 bp
ATGGGGTTTGATGAGACTGCAGTTGTGGCAGTTCGGGTGGAGTTTGTGCTTCTGGTTCTTCTGCTTGGGTGTATGAGCTTCGGCTATGGGGATTCACAGAACGATCAAGGAACACTGCTGGAGATAAAGAAGGCATTTAGGGATGTGGACAATGTTCTCTATGAATGGACAGACCCACCCTCTTTAGATTATTGTGTTTGGAGAGGCGTCACATGTGATAACTCTCTCAATGTCATTGCACTTAATCTATCGGGTTTGAATCTTGGAGGGGAAATCTCATCTGTAATAGGGGATCTCGAAAACCTCCAGTCTATCGATTTGAGGGGGAACCACCTATCCGGCCAGATCCCAGATGAGATTGGTGACTGTTCGGCTCTGCAAAACATTGACTTGTCCTTCAATGAGATATTTGGAGACATACCATTTTCGATATCCAAGTTAAAACAGCTGGAAAATCTGATTTTGAAAAATAATCAATTGATTGGTCCACTTCCTTCGACGTTGTCCCAGATTCCGAATCTGAAGATTTTAGACCTAGCACAGAATAATCTCACTGGGGAAATACCAAGGCTTATATACTGGAATGAAGTTCTACAGTATCTTGGTTTGCGAGGGAACAATTTAGTCGGAACCCTTTCTCCAGATATGTGCCAGTTGACTGGGTTATGGTATTTTGATGTGCGAAACAACAGTTTGACCGGTAGCATTCCTCAAAATATAGGGAACTGCACTGCTTTCCAGGTTTTGGATCTGTCCTACAACCAGCTAACTGGAGAGATTCCATTTAATATCGGGTTCCTGCAAGTAGCCACCTTATCATTACAAGGTAATCAACTTTCTGGGCCAATCCCATCTGTGATCGGCCTAATGCAGGCTCTCGCTGTATTGGATTTGAGCAGCAACGCGTTAAGTGGATCGATCCCTCCTATCCTGGGGAATTTGACTTATACAGAGAAATTGTATTTGCATGGTAACAAGCTTAATGGATCCATTCCCCCAGAGCTTGGACAGATGACAAAGCTACATTATTTGGAATTGAACGATAACCATCTTACGGGATATATTCCACCTGAACTTGGGAAGCTTACGGATTTGTATGACCTAAACGTTGCGAACAACTATCTTCAAGGGCCCATTCCTGATAATCTTAGTTCGTGTACAAATCTGAACAGCCTCAATGTGCATGGGAACAAGTTGAATGGAACCATTCCGCCTGCTCTTCAGAGGCTCGAGAGTATGACTTATCTAAATCTATCCTCCAACCATCTTCGTGGCCCAATTCCTATCGAGCTGTCTCGGATTGGTAACTTGGATACTTTGGATATTTCAAATAACAAACTAAGTGGAACCATTCCTTCATCACTTGGGGATTTGGAACATCTTTTAAAGCTGAATTTGAGTCGAAACCATTTGACGGGATTTATTCCAGGGGAGTTTGGTAATTTAAGGAGTGTTATGGAAATAGACCTTTCAAGTAATCAGCTCACGGGATTAATTCCTCAAGAGCTCAGTCAGCTGCAGAACATGAATTTATTGAGATTAGACCACAACAATATATCCGGGGATGTGGTATCGCTGATAAACTGCTTCAGCCTTTCTGTATTAAATGTATCTTACAACAACTTGGCAGGCGATATTCCCACGAGCAAGAACTTCTCAAGGTTTTCACCAGACAGTTTTATTGGAAACCCTGATCTTTGTGGCTATTGGCTCAATTCTCGATGTCATGAGTCTCGTCCAACAGAGCGAGTGACACTATCTAAAGCTGCTATCCTGGGAATCGCTCTTGGTGCCCTTGTGATTCTTCTCATGATTCTTGTTGCTGCATGCAGGCCATATAATCCAACTCCCTTTCCTGACGGTACATTTGACAAACCAGTTAATTACTCAACTCCGAAGCTGGTGATCCTTAACATGAATATGGCACTTCATGTATACGAGGACATCATGAGGATGACCGAAAACTTGAGCGAGAAGTATATAATTGGTTATGGTGCATCGAGTACAGTGTACAAATGTGTTCTGAAAAATTGTAAGCCAGTGGCTATCAAGAAACTTTACTCGCACTATCCTCAGTGCATGAAGGAGTTTGAGACTGAACTTGCGACAGTTGGAAGCATCAAGCATCGGAATCTGGTGACCCTCCAGGGGTACTCCTTGTCTTCCTCCGGAAACCTTCTCTTTTACGATTACATGGAAAATGGCAGTCTCTGGGATCTCCTTCACGGCCCTTCCAAGAAGAAAAAGCTCGACTGGACAACTCGTCTCCAGATTGCCCTTGGAACAGCCCAAGGGCTTGCCTACCTGCATCATGATTGCAGCCCCAGAATCATACACCGGGATATCAAGTCGTCCAACATTCTACTGGACAAGGATTTTGAAGCTCATTTAACTGATTTCGGCATTGCCAAGAACTTATGCCCCTCAAAGACCCATACGTCTACTTACATAATGGGCACGATTGGCTATATAGATCCTGAGTATGCGAGAACGTCACGCCTCACTGAGAAGTCCGATGTGTATAGTTACGGCATTGTTCTGCTGGAGCTGCTGACGGGAAGGAAAGCCGTAGACAATGAATCCAATCTCCATCATTTGATATTATCTAAGACGGCAAACAATGCTGTCATGGAAACCGTAGATTCTGACGTCACGGCCACATGCACGGACCTCGGAGCAGTAAAGAAGGTTTTCCAGCTTGCCCTTCTGTGCACAAAGCGGCAGCCAACAGACCGGCCAACAATGCATGAAGTAACTCGCGTGCTGGGGAGTCTCGTGCCTTCCCCTGCACCACCAAAACAACCAGCCTCCGCCAACCCACCATCGACACAACACCCGTCTGCCAAAGCGCCGTGCTACGTGGACGAGTACGCGAATCTCAAAACGCCGCACATGCTAAATTGTCCATCCATGAGCACCTCAGATGCCCAGCTGTTTCTCAAGTTTGGAGAGGTAATATCTCAGAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

992

Amino Acids

109.0

Weight (kDa)

5.61

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 73 7.9e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 87 - 179 4e-06 Leucine-rich repeat region
LRR_14 PF23598 271 - 395 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 365 - 423 6.1e-07 Leucine rich repeat
LRR_14 PF23598 383 - 518 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 436 - 495 7.1e-11 Leucine rich repeat
Pkinase PF00069 659 - 919 3.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 661 - 921 1.3e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2437
AatII GACGTC 1 cut(s) 2652
Acc36I ACCTGC 1 cut(s) 2313
AccB1I GGYRCC 1 cut(s) 1790
AccB7I CCANNNNNTGG 2 cut(s) 745, 1004
AccI GTMKAC 3 cut(s) 1935, 2441, 2571
AccII CGCG 3 cut(s) 905, 2760, 2873
AccIII TCCGGA 1 cut(s) 2168
AciI CCGC 4 cut(s) 1211, 2719, 2811, 2888
AclI AACGTT 1 cut(s) 1107
AcoI YGGCCR 3 cut(s) 340, 2655, 2735
AcsI RAATTY 3 cut(s) 937, 1393, 1528
AcuI CTGAAG 3 cut(s) 542, 1205, 1573
AcyI GRCGYC 2 cut(s) 207, 2649
AfaI GTAC 6 cut(s) 1159, 1864, 2005, 2012, 2153, 2870
AfiI CCNNNNNNNGG 8 cut(s) 745, 871, 932, 1004, 1054, 1076, 1414, 2148
AflIII ACRYGT 2 cut(s) 212, 903
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 9 cut(s) 259, 400, 466, 1039, 1127, 1198, 1329, 1473, 2381
AhdI GACNNNNNGTC 1 cut(s) 2262
AjnI CCWGG 5 cut(s) 738, 930, 1426, 1773, 2145
AloI GAACNNNNNNTCC 4 cut(s) 320, 352, 622, 654
Alw21I GWGCWC 3 cut(s) 1509, 2714, 2918
Alw26I GTCTC 8 cut(s) 8, 408, 1299, 1737, 2087, 2213, 2270, 2777
Alw44I GTGCAC 1 cut(s) 2710
AlwNI CAGNNNCTG 1 cut(s) 2211
Ama87I CYCGRG 1 cut(s) 1229
Aor13HI TCCGGA 1 cut(s) 2168
AoxI GGCC 9 cut(s) 340, 842, 862, 1130, 1273, 1832, 2227, 2655, 2735
ApaI GGGCCC 1 cut(s) 1134
ApaLI GTGCAC 1 cut(s) 2710
ApeKI GCWGC 7 cut(s) 897, 1516, 1766, 1823, 2319, 2548, 2722
ApoI RAATTY 3 cut(s) 937, 1393, 1528
ArsI GACNNNNNNTTYG 4 cut(s) 1850, 1882, 2360, 2392
AseI ATTAAT 1 cut(s) 1493
AsiGI ACCGGT 1 cut(s) 698
Asp700I GAANNNNTTC 2 cut(s) 634, 2690
AspLEI GCGC 1 cut(s) 2851
AspS9I GGNCC 7 cut(s) 485, 842, 1130, 1131, 1274, 2228, 2668
AsuC2I CCSGG 2 cut(s) 1561, 2339
AsuHPI GGTGA 4 cut(s) 374, 1659, 1915, 2149
AvaI CYCGRG 1 cut(s) 1229
AvaII GGWCC 2 cut(s) 485, 2668
BaeGI GKGCMC 4 cut(s) 1134, 1795, 2459, 2714
BamHI GGATCC 1 cut(s) 986
BanI GGYRCC 1 cut(s) 1790
BanII GRGCYC 2 cut(s) 1134, 1509
BauI CACGAG 2 cut(s) 1642, 2774
BbsI GAAGAC 1 cut(s) 2154
Bbv12I GWGCWC 3 cut(s) 1509, 2714, 2918
BbvI GCAGC 7 cut(s) 909, 1503, 1753, 1810, 2331, 2535, 2734
BccI CCATC 6 cut(s) 859, 1056, 1272, 2598, 2830, 2914
BceAI ACGGC 6 cut(s) 2242, 2545, 2552, 2628, 2670, 2836
BcgI CGANNNNNNTGC 2 cut(s) 2653, 2687
BciT130I CCWGG 5 cut(s) 740, 932, 1428, 1775, 2147
BciVI GTATCC 1 cut(s) 1306
BcnI CCSGG 2 cut(s) 1561, 2339
BcoDI GTCTC 8 cut(s) 8, 408, 1299, 1737, 2087, 2213, 2270, 2777
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 3 cut(s) 18, 593, 1517
BfoI RGCGCY 1 cut(s) 2852
BfuAI ACCTGC 1 cut(s) 2313
BfuI GTATCC 1 cut(s) 1306
BisI GCNGC 9 cut(s) 898, 1517, 1767, 1824, 2320, 2549, 2720, 2723, 2888
BlsI GCNGC 9 cut(s) 899, 1518, 1768, 1825, 2321, 2550, 2721, 2724, 2889
Bme1390I CCNGG 7 cut(s) 740, 932, 1428, 1561, 1775, 2147, 2339
Bme18I GGWCC 2 cut(s) 485, 2668
BmeRI GACNNNNNGTC 1 cut(s) 2262
BmeT110I CYCGRG 1 cut(s) 1229
BmgT120I GGNCC 7 cut(s) 485, 842, 1130, 1131, 1274, 2228, 2668
BmiI GGNNCC 8 cut(s) 329, 631, 797, 988, 1132, 1204, 1348, 1792
BmrFI CCNGG 7 cut(s) 740, 932, 1428, 1561, 1775, 2147, 2339
BmrI ACTGGG 2 cut(s) 564, 669
BmsI GCATC 5 cut(s) 2006, 2127, 2134, 2317, 2914
BmuI ACTGGG 2 cut(s) 564, 669
BpiI GAAGAC 1 cut(s) 2154
BpmI CTGGAG 7 cut(s) 140, 290, 624, 792, 2129, 2252, 2564
BpuEI CTTGAG 4 cut(s) 1485, 1642, 1987, 2924
BpuMI CCSGG 2 cut(s) 1561, 2339
Bsa29I ATCGAT 2 cut(s) 315, 917
BsaAI YACGTR 1 cut(s) 2860
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 2 cut(s) 207, 2649
BsaJI CCNNGG 9 cut(s) 566, 931, 1427, 1560, 1774, 2146, 2278, 2290, 2671
BsaWI WCCGGW 2 cut(s) 698, 2168
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 8 cut(s) 745, 871, 932, 1004, 1054, 1076, 1414, 2148
Bse118I RCCGGY 2 cut(s) 698, 2733
Bse3DI GCAATG 2 cut(s) 234, 2403
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 2168
BseBI CCWGG 5 cut(s) 740, 932, 1428, 1775, 2147
BseCI ATCGAT 2 cut(s) 315, 917
BseDI CCNNGG 9 cut(s) 566, 931, 1427, 1560, 1774, 2146, 2278, 2290, 2671
BseGI GGATG 4 cut(s) 148, 1570, 1959, 2906
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 8 cut(s) 745, 871, 932, 1004, 1054, 1076, 1414, 2148
BseMI GCAATG 2 cut(s) 234, 2403
BseMII CTCAG 6 cut(s) 1522, 2087, 2469, 2496, 2934, 2975
BseSI GKGCMC 4 cut(s) 1134, 1795, 2459, 2714
BseXI GCAGC 7 cut(s) 909, 1503, 1753, 1810, 2331, 2535, 2734
BseYI CCCAGC 2 cut(s) 2764, 2928
BsgI GTGCAG 2 cut(s) 712, 2772
Bsh1236I CGCG 3 cut(s) 905, 2760, 2873
BshFI GGCC 9 cut(s) 342, 844, 864, 1132, 1275, 1834, 2229, 2657, 2737
BshNI GGYRCC 1 cut(s) 1790
BshTI ACCGGT 1 cut(s) 698
BshVI ATCGAT 2 cut(s) 315, 917
BsiHKAI GWGCWC 3 cut(s) 1509, 2714, 2918
BsiHKCI CYCGRG 1 cut(s) 1229
BsiSI CCGG 6 cut(s) 339, 699, 1560, 2169, 2338, 2734
BslFI GGGAC 1 cut(s) 490
BslI CCNNNNNNNGG 8 cut(s) 745, 871, 932, 1004, 1054, 1076, 1414, 2148
BsmAI GTCTC 8 cut(s) 8, 408, 1299, 1737, 2087, 2213, 2270, 2777
BsmBI CGTCTC 1 cut(s) 2270
BsmFI GGGAC 1 cut(s) 490
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 9 cut(s) 342, 844, 864, 1132, 1275, 1834, 2229, 2657, 2737
BsoBI CYCGRG 1 cut(s) 1229
Bsp120I GGGCCC 1 cut(s) 1130
Bsp1286I GDGCHC 6 cut(s) 1134, 1509, 1795, 2459, 2714, 2918
Bsp13I TCCGGA 1 cut(s) 2168
Bsp1407I TGTACA 2 cut(s) 1157, 2010
BspACI CCGC 4 cut(s) 1211, 2719, 2811, 2888
BspANI GGCC 9 cut(s) 342, 844, 864, 1132, 1275, 1834, 2229, 2657, 2737
BspCNI CTCAG 6 cut(s) 1521, 2086, 2470, 2497, 2933, 2974
BspDI ATCGAT 2 cut(s) 315, 917
BspEI TCCGGA 1 cut(s) 2168
BspFNI CGCG 3 cut(s) 905, 2760, 2873
BspHI TCATGA 4 cut(s) 1726, 1809, 1947, 2311
BspLI GGNNCC 8 cut(s) 329, 631, 797, 988, 1132, 1204, 1348, 1792
BspMAI CTGCAG 2 cut(s) 22, 1521
BspMI ACCTGC 1 cut(s) 2313
BspQI GCTCTTC 1 cut(s) 1224
BspT107I GGYRCC 1 cut(s) 1790
BsrDI GCAATG 2 cut(s) 234, 2403
BsrFI RCCGGY 2 cut(s) 698, 2733
BsrGI TGTACA 2 cut(s) 1157, 2010
BssAI RCCGGY 2 cut(s) 698, 2733
BssECI CCNNGG 9 cut(s) 566, 931, 1427, 1560, 1774, 2146, 2278, 2290, 2671
BssNAI GTATAC 1 cut(s) 1936
BssNI GRCGYC 2 cut(s) 207, 2649
BssSI CACGAG 2 cut(s) 1642, 2774
BssT1I CCWWGG 3 cut(s) 566, 2278, 2290
Bst1107I GTATAC 1 cut(s) 1936
Bst2BI CACGAG 2 cut(s) 1642, 2774
Bst2UI CCWGG 5 cut(s) 740, 932, 1428, 1775, 2147
Bst4CI ACNGT 9 cut(s) 368, 597, 692, 1676, 1862, 2008, 2110, 2638, 2969
Bst6I CTCTTC 1 cut(s) 1224
BstACI GRCGYC 2 cut(s) 207, 2649
BstAPI GCANNNNNTGC 1 cut(s) 2621
BstAUI TGTACA 2 cut(s) 1157, 2010
BstBAI YACGTR 1 cut(s) 2860
BstC8I GCNNGC 8 cut(s) 873, 1215, 1630, 1828, 1832, 2298, 2700, 2762
BstDEI CTNAG 9 cut(s) 1148, 1340, 1508, 2073, 2478, 2505, 2607, 2920, 2961
BstEII GGTNACC 1 cut(s) 2137
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 4 cut(s) 148, 1570, 1959, 2906
BstFNI CGCG 3 cut(s) 905, 2760, 2873
BstH2I RGCGCY 1 cut(s) 2852
BstHHI GCGC 1 cut(s) 2851
BstMAI GTCTC 8 cut(s) 8, 408, 1299, 1737, 2087, 2213, 2270, 2777
BstMWI GCNNNNNNNGC 8 cut(s) 26, 1513, 1790, 2463, 2548, 2621, 2743, 2893
BstNI CCWGG 5 cut(s) 740, 932, 1428, 1775, 2147
BstNSI RCATGY 4 cut(s) 216, 1830, 2664, 2896
BstPI GGTNACC 1 cut(s) 2137
BstSCI CCNGG 7 cut(s) 738, 930, 1426, 1559, 1773, 2145, 2337
BstSFI CTRYAG 3 cut(s) 18, 593, 1517
BstSLI GKGCMC 4 cut(s) 1134, 1795, 2459, 2714
BstUI CGCG 3 cut(s) 905, 2760, 2873
BstV1I GCAGC 7 cut(s) 909, 1503, 1753, 1810, 2331, 2535, 2734
BstV2I GAAGAC 1 cut(s) 2154
BstX2I RGATCY 6 cut(s) 292, 345, 748, 986, 2215, 2473
BstXI CCANNNNNNTGG 3 cut(s) 659, 770, 1271
BstYI RGATCY 6 cut(s) 292, 345, 748, 986, 2215, 2473
BstZ17I GTATAC 1 cut(s) 1936
Bsu15I ATCGAT 2 cut(s) 315, 917
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 9 cut(s) 342, 844, 864, 1132, 1275, 1834, 2229, 2657, 2737
BsuTUI ATCGAT 2 cut(s) 315, 917
BtsCI GGATG 4 cut(s) 148, 1570, 1959, 2906
BtsI GCAGTG 2 cut(s) 113, 729
BtsIMutI CAGTG 8 cut(s) 113, 552, 729, 2013, 2046, 2081, 2502, 2974
BveI ACCTGC 1 cut(s) 2313
Cac8I GCNNGC 8 cut(s) 873, 1215, 1630, 1828, 1832, 2298, 2700, 2762
CaiI CAGNNNCTG 1 cut(s) 2211
CciI TCATGA 4 cut(s) 1726, 1809, 1947, 2311
CfoI GCGC 1 cut(s) 2851
Cfr10I RCCGGY 2 cut(s) 698, 2733
Cfr13I GGNCC 7 cut(s) 485, 842, 1130, 1131, 1274, 2228, 2668
ClaI ATCGAT 2 cut(s) 315, 917
CseI GACGC 1 cut(s) 196
Csp6I GTAC 6 cut(s) 1158, 1863, 2004, 2011, 2152, 2869
CspAI ACCGGT 1 cut(s) 698
CspCI CAANNNNNGTGG 2 cut(s) 1324, 1359
CviQI GTAC 6 cut(s) 1158, 1863, 2004, 2011, 2152, 2869
DdeI CTNAG 9 cut(s) 1148, 1340, 1508, 2073, 2478, 2505, 2607, 2920, 2961
DraI TTTAAA 1 cut(s) 1386
DrdI GACNNNNNNGTC 1 cut(s) 2437
DriI GACNNNNNGTC 1 cut(s) 2262
DseDI GACNNNNNNGTC 1 cut(s) 2437
EaeI YGGCCR 3 cut(s) 340, 2655, 2735
Eam1104I CTCTTC 1 cut(s) 1224
Eam1105I GACNNNNNGTC 1 cut(s) 2262
EarI CTCTTC 1 cut(s) 1224
EciI GGCGGA 2 cut(s) 1200, 2800
Ecl136II GAGCTC 1 cut(s) 1507
Eco130I CCWWGG 3 cut(s) 566, 2278, 2290
Eco24I GRGCYC 2 cut(s) 1134, 1509
Eco32I GATATC 2 cut(s) 432, 2344
Eco47I GGWCC 2 cut(s) 485, 2668
Eco53kI GAGCTC 1 cut(s) 1507
Eco57I CTGAAG 3 cut(s) 542, 1205, 1573
Eco88I CYCGRG 1 cut(s) 1229
Eco91I GGTNACC 1 cut(s) 2137
EcoICRI GAGCTC 1 cut(s) 1507
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 1130
EcoO65I GGTNACC 1 cut(s) 2137
EcoRII CCWGG 5 cut(s) 738, 930, 1426, 1773, 2145
EcoRV GATATC 2 cut(s) 432, 2344
EcoT14I CCWWGG 3 cut(s) 566, 2278, 2290
EcoT22I ATGCAT 1 cut(s) 2748
EcoT38I GRGCYC 2 cut(s) 1134, 1509
ErhI CCWWGG 3 cut(s) 566, 2278, 2290
Esp3I CGTCTC 1 cut(s) 2270
FaqI GGGAC 1 cut(s) 490
FblI GTMKAC 3 cut(s) 1935, 2441, 2571
Fnu4HI GCNGC 9 cut(s) 898, 1517, 1767, 1824, 2320, 2549, 2720, 2723, 2888
FokI GGATG 4 cut(s) 155, 1577, 1966, 2893
FriOI GRGCYC 2 cut(s) 1134, 1509
Fsp4HI GCNGC 9 cut(s) 898, 1517, 1767, 1824, 2320, 2549, 2720, 2723, 2888
FspBI CTAG 1 cut(s) 536
GlaI GCGC 1 cut(s) 2850
GluI GCNGC 9 cut(s) 898, 1517, 1767, 1824, 2320, 2549, 2720, 2723, 2888
GsaI CCCAGC 2 cut(s) 2768, 2932
GsuI CTGGAG 7 cut(s) 140, 290, 624, 792, 2129, 2252, 2564
HaeII RGCGCY 1 cut(s) 2852
HaeIII GGCC 9 cut(s) 342, 844, 864, 1132, 1275, 1834, 2229, 2657, 2737
HapII CCGG 6 cut(s) 339, 699, 1560, 2169, 2338, 2734
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 2851
Hin1I GRCGYC 2 cut(s) 207, 2649
Hin6I GCGC 1 cut(s) 2849
HinP1I GCGC 1 cut(s) 2849
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HindIII AAGCTT 2 cut(s) 977, 1082
HpaII CCGG 6 cut(s) 339, 699, 1560, 2169, 2338, 2734
HphI GGTGA 4 cut(s) 374, 1659, 1915, 2149
Hpy99I CGWCG 1 cut(s) 502
HpyCH4III ACNGT 9 cut(s) 368, 597, 692, 1676, 1862, 2008, 2110, 2638, 2969
HpyCH4IV ACGT 6 cut(s) 500, 1107, 2438, 2492, 2649, 2859
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 1513, 1790, 2463, 2548, 2621, 2743, 2893
HpyF3I CTNAG 9 cut(s) 1148, 1340, 1508, 2073, 2478, 2505, 2607, 2920, 2961
HpySE526I ACGT 6 cut(s) 500, 1107, 2438, 2492, 2649, 2859
Hsp92I GRCGYC 2 cut(s) 207, 2649
HspAI GCGC 1 cut(s) 2849
Kpn2I TCCGGA 1 cut(s) 2168
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 2 cut(s) 2545, 2675
Lsp1109I GCAGC 7 cut(s) 909, 1503, 1753, 1810, 2331, 2535, 2734
LweI GCATC 5 cut(s) 2006, 2127, 2134, 2317, 2914
MaeI CTAG 1 cut(s) 536
MaeII ACGT 6 cut(s) 500, 1107, 2438, 2492, 2649, 2859
MboII GAAGA 8 cut(s) 50, 535, 1115, 1211, 1259, 1796, 2154, 2251
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 6 cut(s) 292, 345, 748, 986, 2215, 2473
MhlI GDGCHC 6 cut(s) 1134, 1509, 1795, 2459, 2714, 2918
MluI ACGCGT 1 cut(s) 903
MlyI GAGTC 3 cut(s) 1408, 1739, 2779
MmeI TCCRAC 6 cut(s) 607, 1284, 1763, 1868, 2092, 2379
Mph1103I ATGCAT 1 cut(s) 2748
MroI TCCGGA 1 cut(s) 2168
MroXI GAANNNNTTC 2 cut(s) 634, 2690
MslI CAYNNNNRTG 3 cut(s) 1269, 1919, 1952
MspA1I CMGCKG 3 cut(s) 448, 1516, 2932
MspI CCGG 6 cut(s) 339, 699, 1560, 2169, 2338, 2734
MspR9I CCNGG 7 cut(s) 740, 932, 1428, 1561, 1775, 2147, 2339
MunI CAATTG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 5 cut(s) 740, 932, 1428, 1775, 2147
MvnI CGCG 3 cut(s) 905, 2760, 2873
MwoI GCNNNNNNNGC 8 cut(s) 26, 1513, 1790, 2463, 2548, 2621, 2743, 2893
NciI CCSGG 2 cut(s) 1561, 2339
NlaIV GGNNCC 8 cut(s) 329, 631, 797, 988, 1132, 1204, 1348, 1792
NmuCI GTSAC 6 cut(s) 208, 362, 1750, 2137, 2493, 2650
NsiI ATGCAT 1 cut(s) 2748
NspI RCATGY 4 cut(s) 216, 1830, 2664, 2896
PaeI GCATGC 1 cut(s) 1830
PaeR7I CTCGAG 1 cut(s) 1229
PagI TCATGA 4 cut(s) 1726, 1809, 1947, 2311
PciI ACATGT 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 1224
PctI GAATGC 1 cut(s) 705
PdmI GAANNNNTTC 2 cut(s) 634, 2690
PflFI GACNNNGTC 1 cut(s) 502
PflMI CCANNNNNTGG 2 cut(s) 745, 1004
PinAI ACCGGT 1 cut(s) 698
PkrI GCNGC 9 cut(s) 899, 1518, 1768, 1825, 2321, 2550, 2721, 2724, 2889
PleI GAGTC 3 cut(s) 1407, 1738, 2778
PpsI GAGTC 3 cut(s) 1407, 1738, 2778
Ppu21I YACGTR 1 cut(s) 2860
PscI ACATGT 1 cut(s) 212
PshBI ATTAAT 1 cut(s) 1493
Psp124BI GAGCTC 1 cut(s) 1509
Psp1406I AACGTT 1 cut(s) 1107
Psp6I CCWGG 5 cut(s) 738, 930, 1426, 1773, 2145
PspEI GGTNACC 1 cut(s) 2137
PspFI CCCAGC 2 cut(s) 2764, 2928
PspGI CCWGG 5 cut(s) 738, 930, 1426, 1773, 2145
PspN4I GGNNCC 8 cut(s) 329, 631, 797, 988, 1132, 1204, 1348, 1792
PspOMI GGGCCC 1 cut(s) 1130
PspPI GGNCC 7 cut(s) 485, 842, 1130, 1131, 1274, 2228, 2668
PstI CTGCAG 2 cut(s) 22, 1521
PstNI CAGNNNCTG 1 cut(s) 2211
PsuI RGATCY 6 cut(s) 292, 345, 748, 986, 2215, 2473
PsyI GACNNNGTC 1 cut(s) 502
PvuII CAGCTG 3 cut(s) 448, 1516, 2932
RsaI GTAC 6 cut(s) 1159, 1864, 2005, 2012, 2153, 2870
RsaNI GTAC 6 cut(s) 1158, 1863, 2004, 2011, 2152, 2869
RseI CAYNNNNRTG 3 cut(s) 1269, 1919, 1952
SacI GAGCTC 1 cut(s) 1509
SapI GCTCTTC 1 cut(s) 1224
SatI GCNGC 9 cut(s) 898, 1517, 1767, 1824, 2320, 2549, 2720, 2723, 2888
Sau96I GGNCC 7 cut(s) 485, 842, 1130, 1131, 1274, 2228, 2668
SchI GAGTC 3 cut(s) 1408, 1739, 2779
ScrFI CCNGG 7 cut(s) 740, 932, 1428, 1561, 1775, 2147, 2339
SduI GDGCHC 6 cut(s) 1134, 1509, 1795, 2459, 2714, 2918
SfaNI GCATC 5 cut(s) 2006, 2127, 2134, 2317, 2914
SfcI CTRYAG 3 cut(s) 18, 593, 1517
Sfr274I CTCGAG 1 cut(s) 1229
SinI GGWCC 2 cut(s) 485, 2668
SlaI CTCGAG 1 cut(s) 1229
SmiMI CAYNNNNRTG 3 cut(s) 1269, 1919, 1952
SmlI CTYRAG 5 cut(s) 1229, 1500, 1657, 1966, 2939
SmoI CTYRAG 5 cut(s) 1229, 1500, 1657, 1966, 2939
SphI GCATGC 1 cut(s) 1830
SsiI CCGC 4 cut(s) 1211, 2719, 2811, 2888
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 1509
StyD4I CCNGG 7 cut(s) 738, 930, 1426, 1559, 1773, 2145, 2337
StyI CCWWGG 3 cut(s) 566, 2278, 2290
TaaI ACNGT 9 cut(s) 368, 597, 692, 1676, 1862, 2008, 2110, 2638, 2969
TaiI ACGT 6 cut(s) 503, 1110, 2441, 2495, 2652, 2862
TaqII GACCGA 1 cut(s) 1973
TatI WGTACW 3 cut(s) 1157, 2003, 2010
TauI GCSGC 2 cut(s) 2722, 2890
TscAI CASTG 8 cut(s) 120, 559, 736, 2013, 2046, 2081, 2509, 2974
TseFI GTSAC 6 cut(s) 208, 362, 1750, 2137, 2493, 2650
TseI GCWGC 7 cut(s) 897, 1516, 1766, 1823, 2319, 2548, 2722
Tsp45I GTSAC 6 cut(s) 208, 362, 1750, 2137, 2493, 2650
TspDTI ATGAA 9 cut(s) 177, 600, 1347, 1541, 1919, 1931, 2096, 2592, 2763
TspGWI ACGGA 2 cut(s) 1103, 2681
TspRI CASTG 8 cut(s) 120, 559, 736, 2013, 2046, 2081, 2509, 2974
Tth111I GACNNNGTC 1 cut(s) 502
Van91I CCANNNNNTGG 2 cut(s) 745, 1004
VneI GTGCAC 1 cut(s) 2710
VpaK11BI GGWCC 2 cut(s) 485, 2668
VspI ATTAAT 1 cut(s) 1493
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 3 cut(s) 937, 1393, 1528
XceI RCATGY 4 cut(s) 216, 1830, 2664, 2896
XcmI CCANNNNNNNNNTGG 2 cut(s) 2276, 2362
XhoI CTCGAG 1 cut(s) 1229
XmiI GTMKAC 3 cut(s) 1935, 2441, 2571
XmnI GAANNNNTTC 2 cut(s) 634, 2690
XspI CTAG 1 cut(s) 536
ZraI GACGTC 1 cut(s) 2650
Zsp2I ATGCAT 1 cut(s) 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.