Rw6G044210
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016560
Physical Location & Seq
Forward (+)
67371060 .. 67378060
7001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044210.1

Sequence Viewer

Length: 2955 bp
ATGGCATTTCGGTTTGACTTGTTGCTGCTTCTGGGTTTGCTCGTTTGTGTGAGCTTTGGTAATGTGGACTCAGATGAAGGAGCAACACTGCTGGAGATAAAGAAGTCGTTTAGGGATGTGGACAATGTTCTATACGACTGGAGAGACTCACCGTCTTTAGACTATTGTGTTTGGAGAGGCGTCACATGTGACAATGTCACCTTCAATGTCATAGCACTCAATCTATCAGGTCTAAATCTTGATGGAGAAATCTCACCTGCAATAGGAAATCTCAAGGGCCTCCAGTCCATTGATTTGAGAGGGAACCGACTTTCAGGCCAGATCCCAGATGAGATTGGTGACTGTTCGTCTCTGAGATACCTGGACCTGTCCTTCAATGAGATATATGGAGACATCCCATTTTCAATATCCAAGTTGAAGCAACTGGAAAGCATAATGTTGAAAAATAATCAGTTGATTGGACCACTCCCTTCAACACTGTCCCAGATTCCGAACCTGAAGATGTTAGACCTAGCCCAGAATAATCTCAGTGGGGAAATACCAAGGCTTATTTACTGGAATGAAGTTCTACAATATCTTGGTTTGCGAGGAAACAATTTAGTTGGGAAACTTTCTCCAGATATGTGCCAGTTAACTGGGTTATGGTATTTTGATGTGCGAAACAACAGTTTGACCGGTAGTATTCCTCAAAATATAGGGAACTGCACTGCCTTCCAAGTCTTGGACCTGTCCTATAACCAGTTAACTGGAGAGATTCCTTTCAACATTGGGTTCCTGCAAGTAGCCACCTTATCATTGCAGGGTAATCAACTTTCTGGGCCTATCCCTTCCGTTATTGGCCTGATGCAGGCACTCGCTGTATTAGATTTGAGTGGCAACATGCTAAGTGGACCGATTCCTCCTATTCTGGGGAATTTGACTTATACAGAGAAATTGTACTTGCATGCCAACAAGCTTACAGGATCAATTCCCCCAGAGCTCGGACAAATGGCACAGCTTCATTATTTGGTGTTGAATGATAACCATCTTACTGGAAAAATTCCACCTGAACTTGGGAAGCTTACCAACTTGTTTGACCTAAATGTTGCTAACAACAATCTTCAAGGGCCCATCCCTGATAATATTAGCTCATGTACAAATCTCAACACCCTCAATGTGCATGGGAACAAATTGAATGGAACCATCCCGCCTGCTCTCCAGAGGCTGGAGAGTATGACTTATCTAAATCTGTCCTCCAACAATCTTCGTGGCTCAATACCAATTGAGCTATCTCGGATTGGCAATTTGGATACTTTGGATCTTTCTAATAACAAGTTTACTGGATCCATACCTTCTTCGCTTGGTGATTTGGAACATCTTCTTAAGCTGAATTTGAGTAGAAATCATATGACAGGTTTTATTCCAGCAGAGTTTGGTAATCTAAGGAGTGTTGTGGACATAGATCTTTCAAATAATCGGCTCACAGGAATGATTCCTCAGGAGCTCAGTCAGCTGCAGAATTTGTTCTCACTGAGGCTAGAGCACAACAATATATCAGGGGAAGTGGTGTCTTTGATCAATTGCCTCAGTCTTGCAGTGCTAAATGTGTCTTATAACAACCTGGCGGGAGATATTCCCACATCCAATAACTTCTCTAGGTTTTCATCTGACAGCTTTGTTGGAAATCCTGATCTTTGTGGCTATTGGCTCAATTCTCCGTGTCATGAGTCCCGTCCAACAGAGAGAGTTGCATTATCTAAAGCGGCTATCCTGGGCATTGCTCTTGGTGCCCTTGTGATTCTTCTCATGATCCTTATTGCTGCCTGCCGCCCTTACCATCCAACTCCTTTTCCTGACGGTACACTTGACAAACCAGCAGTTAATTACTCAACACCAAAGCTAGTGATTCTTAACATGAATATGGCACTTCATGTGTATGAGGATATCATGAGGATGACTGAAAACTTGAGTGAGAAATATATAATTGGTTATGGCGCATCAAGCACGGTATACAAATGTGTTCTTAAGAATTGTAAGCCAGTGGCGATCAAGAGACTCTACTCTCATTATCCACAGTGCTTGAAGGAATTTGAGACAGAACTTGGAACAGTTGGCAGCATCAAGCATCGAAATCTAGTCAGCCTCCAAGGATACTCCTTGTCTTCTTCTGGAAACCTTCTCTTTTATGATTACATGGAAAATGGCAGTCTCTGGGATCTCCTTCATGGCCCAACTACCAAGAAGAAGAAGCTTGACTGGGAAACTCGTATCCAGATAGCACTCGGAGCAGCCCAAGGGCTTGCCTATCTCCATCATGATTGTAGTCCACGTATCATACACAGGGATGTGAAGTCGTCTAACATTCTGTTGGACAAGGATTTCGAGGCTCATTTAACTGATTTTGGTATTGCCAAGAGCTTATGCACGTCAAAGACCCATACATCTACTTGTGTAATGGGCACCATTGGCTACATAGACCCCGAGTATGCTCGAACTTCCCGCCTCACTGAGAAGTCGGACGTTTATAGTTATGGGATTGTCCTGCTGGAGTTGCTAACAGGAAGGAAGGCTGTAGACAATGAATGCAATCTCCATCAATTGATCTTATCAAAGACAGCGAACAATGCTGTGATGGAAACTGTTGATCCAGAGATCATGGCCACATGCATGGACCTTGGAGCAGTGAAGAAGGTTTTTCAGCTTGCACTCCTATGCACAAAGAGGCAGCCGACAGACCGGCCCACTATGCATGAAATAACACGAGTTTTGGGTAGCCTTGTGCCATCACCTACACTGCCGAAACAAGCAACCCTCAATCCTCCATCCACTACACTCTCATCTGTGAAAGCGCCGTGCTACGTGGATGAGTATGCAAACCTCAAAACCCCACACATGCTCAATTGCCCATCTATGAGTACCTCAGACGCCCAGCTCTTTCTCAAGTTTGGAGAGGTAATTTCTCAGAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

984

Amino Acids

108.3

Weight (kDa)

5.87

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 64 7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 84 - 172 1.8e-06 Leucine-rich repeat region
LRR_8 PF13855 88 - 128 4.7e-06 Leucine rich repeat
LRR_8 PF13855 379 - 439 4.5e-07 Leucine rich repeat
LRR_14 PF23598 399 - 511 4.4e-09 Leucine-rich repeat region
LRR_8 PF13855 428 - 487 1.8e-09 Leucine rich repeat
LRR_8 PF13855 452 - 511 2.4e-08 Leucine rich repeat
Pkinase PF00069 652 - 913 3e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 654 - 915 6.8e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AarI CACCTGC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 2 cut(s) 1766, 2438
AccB7I CCANNNNNTGG 2 cut(s) 721, 1204
AccI GTMKAC 2 cut(s) 1989, 2553
AciI CCGC 5 cut(s) 1187, 1604, 1742, 1807, 2479
AclWI GGATC 8 cut(s) 316, 970, 1305, 1317, 1330, 1783, 2202, 2618
AcoI YGGCCR 1 cut(s) 2637
AcsI RAATTY 5 cut(s) 913, 1038, 1369, 1498, 2066
AcuI CTGAAG 1 cut(s) 518
AcyI GRCGYC 2 cut(s) 180, 2904
AfaI GTAC 4 cut(s) 938, 1135, 1840, 2896
AfiI CCNNNNNNNGG 7 cut(s) 263, 721, 847, 908, 980, 1052, 1204
AflII CTTAAG 2 cut(s) 1361, 2003
AflIII ACRYGT 1 cut(s) 185
AgeI ACCGGT 1 cut(s) 674
AhdI GACNNNNNGTC 2 cut(s) 151, 346
AjiI CACGTC 1 cut(s) 2406
AjnI CCWGG 3 cut(s) 360, 1599, 1749
Alw21I GWGCWC 3 cut(s) 981, 1485, 1524
Alw26I GTCTC 6 cut(s) 138, 354, 384, 2026, 2066, 2192
AlwI GGATC 8 cut(s) 316, 970, 1305, 1317, 1330, 1783, 2202, 2618
AlwNI CAGNNNCTG 2 cut(s) 1204, 2190
Ama87I CYCGRG 1 cut(s) 2459
AoxI GGCC 8 cut(s) 277, 316, 818, 838, 1106, 2206, 2637, 2717
ApaI GGGCCC 1 cut(s) 1110
ApeKI GCWGC 6 cut(s) 25, 1492, 1799, 2094, 2267, 2704
ApoI RAATTY 5 cut(s) 913, 1038, 1369, 1498, 2066
ArsI GACNNNNNNTTYG 2 cut(s) 2342, 2374
AsiGI ACCGGT 1 cut(s) 674
Asp700I GAANNNNTTC 2 cut(s) 1356, 1502
AspLEI GCGC 2 cut(s) 1976, 2830
AsuHPI GGTGA 6 cut(s) 141, 190, 246, 350, 1355, 2757
AvaI CYCGRG 1 cut(s) 2459
AvaII GGWCC 5 cut(s) 364, 461, 724, 890, 2650
AxyI CCTNAGG 1 cut(s) 1476
BaeGI GKGCMC 3 cut(s) 1110, 1771, 2441
BalI TGGCCA 1 cut(s) 2639
BamHI GGATCC 1 cut(s) 1322
BanI GGYRCC 2 cut(s) 1766, 2438
BanII GRGCYC 3 cut(s) 981, 1110, 1485
BauI CACGAG 1 cut(s) 2739
BbsI GAAGAC 1 cut(s) 2133
Bbv12I GWGCWC 3 cut(s) 981, 1485, 1524
BbvI GCAGC 6 cut(s) 12, 1479, 1786, 2106, 2279, 2716
BceAI ACGGC 1 cut(s) 2815
BciT130I CCWGG 3 cut(s) 362, 1601, 1751
BciVI GTATCC 3 cut(s) 1282, 2123, 2258
BclI TGATCA 1 cut(s) 1554
BcoDI GTCTC 6 cut(s) 138, 354, 384, 2026, 2066, 2192
BfaI CTAG 5 cut(s) 512, 1517, 1635, 1880, 2114
BfmI CTRYAG 2 cut(s) 1493, 2550
BfoI RGCGCY 1 cut(s) 2831
BfrI CTTAAG 2 cut(s) 1361, 2003
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 3 cut(s) 1282, 2123, 2258
BglII AGATCT 1 cut(s) 1441
BisI GCNGC 8 cut(s) 26, 1493, 1743, 1800, 1807, 2095, 2268, 2705
BlsI GCNGC 8 cut(s) 27, 1494, 1744, 1801, 1808, 2096, 2269, 2706
Bme1390I CCNGG 3 cut(s) 362, 1601, 1751
Bme18I GGWCC 5 cut(s) 364, 461, 724, 890, 2650
BmeRI GACNNNNNGTC 2 cut(s) 151, 346
BmeT110I CYCGRG 1 cut(s) 2459
BmgBI CACGTC 1 cut(s) 2406
BmiI GGNNCC 7 cut(s) 305, 773, 1108, 1180, 1324, 1768, 2440
BmrFI CCNGG 3 cut(s) 362, 1601, 1751
BmrI ACTGGG 2 cut(s) 645, 2245
BmsI GCATC 4 cut(s) 834, 1985, 2106, 2113
BmuI ACTGGG 2 cut(s) 645, 2245
BpiI GAAGAC 1 cut(s) 2133
BpmI CTGGAG 8 cut(s) 113, 160, 266, 600, 768, 1181, 1226, 2546
BpuEI CTTGAG 3 cut(s) 257, 1966, 2903
BsaAI YACGTR 2 cut(s) 2309, 2839
BsaBI GATNNNNATC 1 cut(s) 1023
BsaHI GRCGYC 2 cut(s) 180, 2904
BsaJI CCNNGG 5 cut(s) 542, 1750, 2125, 2272, 2653
BsaWI WCCGGW 1 cut(s) 674
Bsc4I CCNNNNNNNGG 7 cut(s) 263, 721, 847, 908, 980, 1052, 1204
Bse118I RCCGGY 2 cut(s) 674, 2715
Bse21I CCTNAGG 1 cut(s) 1476
Bse3DI GCAATG 2 cut(s) 794, 1755
Bse8I GATNNNNATC 1 cut(s) 1023
BseBI CCWGG 3 cut(s) 362, 1601, 1751
BseDI CCNNGG 5 cut(s) 542, 1750, 2125, 2272, 2653
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 7 cut(s) 263, 721, 847, 908, 980, 1052, 1204
BseMI GCAATG 2 cut(s) 794, 1755
BseSI GKGCMC 3 cut(s) 1110, 1771, 2441
BseXI GCAGC 6 cut(s) 12, 1479, 1786, 2106, 2279, 2716
BseYI CCCAGC 1 cut(s) 2907
BsgI GTGCAG 1 cut(s) 688
BshFI GGCC 8 cut(s) 279, 318, 820, 840, 1108, 2208, 2639, 2719
BshNI GGYRCC 2 cut(s) 1766, 2438
BshTI ACCGGT 1 cut(s) 674
BsiHKAI GWGCWC 3 cut(s) 981, 1485, 1524
BsiHKCI CYCGRG 1 cut(s) 2459
BsiSI CCGG 2 cut(s) 675, 2716
BslFI GGGAC 2 cut(s) 466, 1693
BslI CCNNNNNNNGG 7 cut(s) 263, 721, 847, 908, 980, 1052, 1204
BsmAI GTCTC 6 cut(s) 138, 354, 384, 2026, 2066, 2192
BsmBI CGTCTC 1 cut(s) 354
BsmFI GGGAC 2 cut(s) 466, 1693
BsmI GAATGC 1 cut(s) 2567
BsnI GGCC 8 cut(s) 279, 318, 820, 840, 1108, 2208, 2639, 2719
BsoBI CYCGRG 1 cut(s) 2459
Bsp120I GGGCCC 1 cut(s) 1106
Bsp1286I GDGCHC 6 cut(s) 981, 1110, 1485, 1524, 1771, 2441
Bsp1407I TGTACA 1 cut(s) 1133
BspACI CCGC 5 cut(s) 1187, 1604, 1742, 1807, 2479
BspANI GGCC 8 cut(s) 279, 318, 820, 840, 1108, 2208, 2639, 2719
BspHI TCATGA 4 cut(s) 1702, 1785, 1926, 2293
BspLI GGNNCC 7 cut(s) 305, 773, 1108, 1180, 1324, 1768, 2440
BspMAI CTGCAG 1 cut(s) 1497
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 8 cut(s) 316, 970, 1305, 1317, 1330, 1783, 2202, 2618
BspT107I GGYRCC 2 cut(s) 1766, 2438
BspTI CTTAAG 2 cut(s) 1361, 2003
BsrDI GCAATG 2 cut(s) 794, 1755
BsrFI RCCGGY 2 cut(s) 674, 2715
BsrGI TGTACA 1 cut(s) 1133
BssAI RCCGGY 2 cut(s) 674, 2715
BssECI CCNNGG 5 cut(s) 542, 1750, 2125, 2272, 2653
BssNAI GTATAC 1 cut(s) 1990
BssNI GRCGYC 2 cut(s) 180, 2904
BssSI CACGAG 1 cut(s) 2739
BssT1I CCWWGG 4 cut(s) 542, 2125, 2272, 2653
Bst1107I GTATAC 1 cut(s) 1990
Bst2BI CACGAG 1 cut(s) 2739
Bst2UI CCWGG 3 cut(s) 362, 1601, 1751
BstACI GRCGYC 2 cut(s) 180, 2904
BstAFI CTTAAG 2 cut(s) 1361, 2003
BstAUI TGTACA 1 cut(s) 1133
BstBAI YACGTR 2 cut(s) 2309, 2839
BstC8I GCNNGC 6 cut(s) 849, 945, 1191, 1804, 2280, 2682
BstENI CCTNNNNNAGG 2 cut(s) 261, 845
BstH2I RGCGCY 1 cut(s) 2831
BstHHI GCGC 2 cut(s) 1976, 2830
BstMAI GTCTC 6 cut(s) 138, 354, 384, 2026, 2066, 2192
BstMWI GCNNNNNNNGC 8 cut(s) 1489, 1766, 1980, 2264, 2445, 2530, 2603, 2725
BstNI CCWGG 3 cut(s) 362, 1601, 1751
BstNSI RCATGY 5 cut(s) 189, 883, 947, 2646, 2875
BstSCI CCNGG 3 cut(s) 360, 1599, 1749
BstSFI CTRYAG 2 cut(s) 1493, 2550
BstSLI GKGCMC 3 cut(s) 1110, 1771, 2441
BstV1I GCAGC 6 cut(s) 12, 1479, 1786, 2106, 2279, 2716
BstV2I GAAGAC 1 cut(s) 2133
BstX2I RGATCY 5 cut(s) 321, 1297, 1322, 1441, 2194
BstXI CCANNNNNNTGG 4 cut(s) 635, 746, 1031, 2647
BstYI RGATCY 5 cut(s) 321, 1297, 1322, 1441, 2194
BstZ17I GTATAC 1 cut(s) 1990
Bsu36I CCTNAGG 1 cut(s) 1476
BsuI GTATCC 3 cut(s) 1282, 2123, 2258
BsuRI GGCC 8 cut(s) 279, 318, 820, 840, 1108, 2208, 2639, 2719
BtrI CACGTC 1 cut(s) 2406
BtsI GCAGTG 5 cut(s) 86, 705, 1581, 2667, 2771
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 6 cut(s) 849, 945, 1191, 1804, 2280, 2682
CaiI CAGNNNCTG 2 cut(s) 1204, 2190
CciI TCATGA 4 cut(s) 1702, 1785, 1926, 2293
CfoI GCGC 2 cut(s) 1976, 2830
Cfr10I RCCGGY 2 cut(s) 674, 2715
CseI GACGC 2 cut(s) 169, 2912
Csp6I GTAC 4 cut(s) 937, 1134, 1839, 2895
CspAI ACCGGT 1 cut(s) 674
CspCI CAANNNNNGTGG 4 cut(s) 1228, 1263, 2040, 2075
CviQI GTAC 4 cut(s) 937, 1134, 1839, 2895
DriI GACNNNNNGTC 2 cut(s) 151, 346
EaeI YGGCCR 1 cut(s) 2637
Eam1105I GACNNNNNGTC 2 cut(s) 151, 346
Ecl136II GAGCTC 2 cut(s) 979, 1483
Eco130I CCWWGG 4 cut(s) 542, 2125, 2272, 2653
Eco24I GRGCYC 3 cut(s) 981, 1110, 1485
Eco32I GATATC 1 cut(s) 1924
Eco47I GGWCC 5 cut(s) 364, 461, 724, 890, 2650
Eco53kI GAGCTC 2 cut(s) 979, 1483
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 1476
Eco88I CYCGRG 1 cut(s) 2459
EcoICRI GAGCTC 2 cut(s) 979, 1483
EcoNI CCTNNNNNAGG 2 cut(s) 261, 845
EcoO109I RGGNCCY 2 cut(s) 277, 1106
EcoRII CCWGG 3 cut(s) 360, 1599, 1749
EcoRV GATATC 1 cut(s) 1924
EcoT14I CCWWGG 4 cut(s) 542, 2125, 2272, 2653
EcoT22I ATGCAT 2 cut(s) 2648, 2730
EcoT38I GRGCYC 3 cut(s) 981, 1110, 1485
ErhI CCWWGG 4 cut(s) 542, 2125, 2272, 2653
Esp3I CGTCTC 1 cut(s) 354
FaqI GGGAC 2 cut(s) 466, 1693
FauI CCCGC 3 cut(s) 1194, 1597, 2486
FauNDI CATATG 1 cut(s) 1386
FbaI TGATCA 1 cut(s) 1554
FblI GTMKAC 2 cut(s) 1989, 2553
Fnu4HI GCNGC 8 cut(s) 26, 1493, 1743, 1800, 1807, 2095, 2268, 2705
FriOI GRGCYC 3 cut(s) 981, 1110, 1485
Fsp4HI GCNGC 8 cut(s) 26, 1493, 1743, 1800, 1807, 2095, 2268, 2705
FspBI CTAG 5 cut(s) 512, 1517, 1635, 1880, 2114
GlaI GCGC 2 cut(s) 1975, 2829
GluI GCNGC 8 cut(s) 26, 1493, 1743, 1800, 1807, 2095, 2268, 2705
GsaI CCCAGC 1 cut(s) 2911
GsuI CTGGAG 8 cut(s) 113, 160, 266, 600, 768, 1181, 1226, 2546
HaeII RGCGCY 1 cut(s) 2831
HaeIII GGCC 8 cut(s) 279, 318, 820, 840, 1108, 2208, 2639, 2719
HapII CCGG 2 cut(s) 675, 2716
HgaI GACGC 2 cut(s) 169, 2912
HhaI GCGC 2 cut(s) 1976, 2830
Hin1I GRCGYC 2 cut(s) 180, 2904
Hin6I GCGC 2 cut(s) 1974, 2828
HinP1I GCGC 2 cut(s) 1974, 2828
HincII GTYRAC 2 cut(s) 633, 744
HindII GTYRAC 2 cut(s) 633, 744
HindIII AAGCTT 3 cut(s) 953, 1058, 2228
HpaI GTTAAC 2 cut(s) 633, 744
HpaII CCGG 2 cut(s) 675, 2716
HphI GGTGA 6 cut(s) 141, 190, 246, 350, 1355, 2757
HpyCH4IV ACGT 4 cut(s) 2308, 2405, 2499, 2838
HpyF10VI GCNNNNNNNGC 8 cut(s) 1489, 1766, 1980, 2264, 2445, 2530, 2603, 2725
HpySE526I ACGT 4 cut(s) 2308, 2405, 2499, 2838
Hsp92I GRCGYC 2 cut(s) 180, 2904
HspAI GCGC 2 cut(s) 1974, 2828
Ksp22I TGATCA 1 cut(s) 1554
KspAI GTTAAC 2 cut(s) 633, 744
LmnI GCTCC 4 cut(s) 80, 1480, 2264, 2657
Lsp1109I GCAGC 6 cut(s) 12, 1479, 1786, 2106, 2279, 2716
LweI GCATC 4 cut(s) 834, 1985, 2106, 2113
MaeI CTAG 5 cut(s) 512, 1517, 1635, 1880, 2114
MaeII ACGT 4 cut(s) 2308, 2405, 2499, 2838
MaeIII GTNAC 4 cut(s) 181, 188, 196, 338
MfeI CAATTG 4 cut(s) 1260, 1558, 2576, 2878
MflI RGATCY 5 cut(s) 321, 1297, 1322, 1441, 2194
MhlI GDGCHC 6 cut(s) 981, 1110, 1485, 1524, 1771, 2441
MlsI TGGCCA 1 cut(s) 2639
MluNI TGGCCA 1 cut(s) 2639
MlyI GAGTC 4 cut(s) 62, 140, 1715, 2028
MmeI TCCRAC 6 cut(s) 1260, 1639, 1739, 1844, 2328, 2475
Mox20I TGGCCA 1 cut(s) 2639
Mph1103I ATGCAT 2 cut(s) 2648, 2730
MroXI GAANNNNTTC 2 cut(s) 1356, 1502
MscI TGGCCA 1 cut(s) 2639
MseI TTAA 7 cut(s) 632, 743, 1362, 1860, 1890, 2004, 2372
MslI CAYNNNNRTG 7 cut(s) 1466, 1898, 1914, 1931, 2322, 2645, 2689
Msp20I TGGCCA 1 cut(s) 2639
MspA1I CMGCKG 1 cut(s) 1492
MspCI CTTAAG 2 cut(s) 1361, 2003
MspI CCGG 2 cut(s) 675, 2716
MspR9I CCNGG 3 cut(s) 362, 1601, 1751
MunI CAATTG 4 cut(s) 1260, 1558, 2576, 2878
Mva1269I GAATGC 1 cut(s) 2567
MvaI CCWGG 3 cut(s) 362, 1601, 1751
MwoI GCNNNNNNNGC 8 cut(s) 1489, 1766, 1980, 2264, 2445, 2530, 2603, 2725
NdeI CATATG 1 cut(s) 1386
NlaIV GGNNCC 7 cut(s) 305, 773, 1108, 1180, 1324, 1768, 2440
NmuCI GTSAC 4 cut(s) 181, 188, 196, 338
NsiI ATGCAT 2 cut(s) 2648, 2730
NspI RCATGY 5 cut(s) 189, 883, 947, 2646, 2875
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 4 cut(s) 1702, 1785, 1926, 2293
PaqCI CACCTGC 1 cut(s) 265
PciI ACATGT 1 cut(s) 185
PctI GAATGC 1 cut(s) 2567
PdmI GAANNNNTTC 2 cut(s) 1356, 1502
PfeI GAWTC 6 cut(s) 487, 754, 895, 1471, 1777, 1885
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 2 cut(s) 721, 1204
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 8 cut(s) 27, 1494, 1744, 1801, 1808, 2096, 2269, 2706
PleI GAGTC 4 cut(s) 62, 140, 1714, 2028
PpsI GAGTC 4 cut(s) 62, 140, 1714, 2028
Ppu21I YACGTR 2 cut(s) 2309, 2839
PscI ACATGT 1 cut(s) 185
PsiI TTATAA 1 cut(s) 1593
Psp124BI GAGCTC 2 cut(s) 981, 1485
Psp6I CCWGG 3 cut(s) 360, 1599, 1749
PspFI CCCAGC 1 cut(s) 2907
PspGI CCWGG 3 cut(s) 360, 1599, 1749
PspN4I GGNNCC 7 cut(s) 305, 773, 1108, 1180, 1324, 1768, 2440
PspOMI GGGCCC 1 cut(s) 1106
PstI CTGCAG 1 cut(s) 1497
PstNI CAGNNNCTG 2 cut(s) 1204, 2190
PsuI RGATCY 5 cut(s) 321, 1297, 1322, 1441, 2194
PsyI GACNNNGTC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 1492
RsaI GTAC 4 cut(s) 938, 1135, 1840, 2896
RsaNI GTAC 4 cut(s) 937, 1134, 1839, 2895
RseI CAYNNNNRTG 7 cut(s) 1466, 1898, 1914, 1931, 2322, 2645, 2689
SacI GAGCTC 2 cut(s) 981, 1485
SaqAI TTAA 7 cut(s) 632, 743, 1362, 1860, 1890, 2004, 2372
SatI GCNGC 8 cut(s) 26, 1493, 1743, 1800, 1807, 2095, 2268, 2705
SchI GAGTC 4 cut(s) 62, 140, 1715, 2028
ScrFI CCNGG 3 cut(s) 362, 1601, 1751
SduI GDGCHC 6 cut(s) 981, 1110, 1485, 1524, 1771, 2441
SfaNI GCATC 4 cut(s) 834, 1985, 2106, 2113
SfcI CTRYAG 2 cut(s) 1493, 2550
SinI GGWCC 5 cut(s) 364, 461, 724, 890, 2650
SmiMI CAYNNNNRTG 7 cut(s) 1466, 1898, 1914, 1931, 2322, 2645, 2689
SmlI CTYRAG 5 cut(s) 272, 1361, 1945, 2003, 2918
SmoI CTYRAG 5 cut(s) 272, 1361, 1945, 2003, 2918
SphI GCATGC 1 cut(s) 947
SsiI CCGC 5 cut(s) 1187, 1604, 1742, 1807, 2479
SspI AATATT 1 cut(s) 1123
SspMI CTAG 5 cut(s) 512, 1517, 1635, 1880, 2114
SstI GAGCTC 2 cut(s) 981, 1485
StyD4I CCNGG 3 cut(s) 360, 1599, 1749
StyI CCWWGG 4 cut(s) 542, 2125, 2272, 2653
TaiI ACGT 4 cut(s) 2311, 2408, 2502, 2841
TaqI TCGA 3 cut(s) 2107, 2361, 2470
TaqII GACCGA 1 cut(s) 907
TatI WGTACW 2 cut(s) 936, 1133
TauI GCSGC 2 cut(s) 1745, 1809
TfiI GAWTC 6 cut(s) 487, 754, 895, 1471, 1777, 1885
Tru1I TTAA 7 cut(s) 632, 743, 1362, 1860, 1890, 2004, 2372
Tru9I TTAA 7 cut(s) 632, 743, 1362, 1860, 1890, 2004, 2372
TseFI GTSAC 4 cut(s) 181, 188, 196, 338
TseI GCWGC 6 cut(s) 25, 1492, 1799, 2094, 2267, 2704
Tsp45I GTSAC 4 cut(s) 181, 188, 196, 338
TspDTI ATGAA 9 cut(s) 90, 576, 989, 1632, 1898, 1910, 2192, 2574, 2745
TspGWI ACGGA 2 cut(s) 820, 1686
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 2 cut(s) 721, 1204
Vha464I CTTAAG 2 cut(s) 1361, 2003
VpaK11BI GGWCC 5 cut(s) 364, 461, 724, 890, 2650
XagI CCTNNNNNAGG 2 cut(s) 261, 845
XapI RAATTY 5 cut(s) 913, 1038, 1369, 1498, 2066
XceI RCATGY 5 cut(s) 189, 883, 947, 2646, 2875
XmiI GTMKAC 2 cut(s) 1989, 2553
XmnI GAANNNNTTC 2 cut(s) 1356, 1502
XspI CTAG 5 cut(s) 512, 1517, 1635, 1880, 2114
Zsp2I ATGCAT 2 cut(s) 2648, 2730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.