FvH4_2g31650

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013905
Physical Location & Seq
Forward (+)
24219624 .. 24222651
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31650.t1

Sequence Viewer

Length: 1347 bp
ATGGGTGCTTCAGGCAAATGGGTGAAGTCCCTTATAGGTATCAAAAAACCTGACAAAGATGACCAAGAAAAGGTAGGTGGGAAGAATAAGAAGTGGAAGCTATGGAGGAATTCGTCAGGGGATTTCGGGTCTTCTTGGAAAGGATTCAAAGGGAGTCACAGAGCTGCTTCTGAAGGGTCTGATTCGCCAAGAAGGGCTGAAGCGTACACTGCTGCTGTGGCTACAGTGGTTCGAGCTCACCCTAGAGATTTCAGAGTTGTCAGGCAAGAATGGGCTGCTATCAGAATCCAAACGGCTTTCCGAGGCTTCCTGGCAAGGAGGGCTTTGAAGGCGTTGAGAGGGATTGTGAGGCTTCAAGCTCTGGTTCGGGGAAGACAAGTGAGGAAGCAAGCGGCTGTAACACTGAGGTGCATGCAGGCTCTAGTTCGAGTTCAGGCACGTGTGAGGGCTCGCCGCGTGCGAATGTCCATTGAAGGCCAAGCTGTGCAGAACATGCTCAATGAGCGCCGCACTCAAGCTGATCTCTTAAAAGAAGCTGAGGAAGGGTGGTGTGATAGCAAGGGAACTATAGATGATGTTAAAGCAAAGATTCAAATGAGGCAAGAAGGAGCCTTCAAAAGGGAGAGAGCAATTGCCTACTCTCTTGCTCAAAAGCAATGGAGATCAACCCCAGATTCGAATTCACGCATCAATTCCTCAGTTTACTCTCTCAATAAGAACGAATTCGACAGGAACAGCTGGGGATGGAGTTGGTTAGAGCGCTGGATGTCAGCTAAGCCATGGGAGACTAGGTTGATGGAACAATCCAACAGTGATCCATCTGAGTTCACACCGCCTCCAAAGAAAATTGCAGACCCTTTTGTTGGCATCGGCAAACATTCGAAATCTTCAGAGCCACCATGCTCAGTCAAAGTGAGAAGGAACAATGTCACCACTAGGATATCGGCAAAGCCACCACACCTTGGGCAAGTGACTACTCGCTCATCGTCGAGCCCAAGTTCAGAGTTTAGGTATGATGACAGCCCTGGCTCATCTTCACTTACATCCACAACACCTGTTTCAGGAAACACCGGACTGGCTTCGGATAGAACAGATGAGAGCAGTAACTTTAGGCCAAGTTACATGAACCTGACTGAGTCAACTAAGGCAAAGCAGAAAGCAGCAAGTTATCAATCTCACAGAACTCAAAGGCAATCCATGGATGAGTTTCAGTTTCTCAAGAGGTCTGGGGCATTCTCTAATGGGGATTCCAAAAGTAGTGCTGGTTCTGAACCACCATCAGTCCATTTCTCAAAGCCGCTTGGCATGCCAACCCGCTTGGATAAGAACACTACGAAATTAAGGTGA

Protein Analysis

449

Amino Acids

50.28

Weight (kDa)

10.59

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26180
fragaria_vesca FvH4_2g31650
malus_domestica MD08G1064300.v1.1 MD15G1057400.v1.1
prunus_persica Prupe.1G409900_v2.0.a1
pyrus_communis pycom08g05280
rosa_chinensis RchiOBHm_Chr6g0311171
rosa_laevigata RLG00000010430
rosa_multiflora Rmu_sc0008967.1_g000015 Rmu_sc0016962.1_g000004
rosa_roxburghii Rroxscaffold_7G00157940
rosa_rugosa Rorug06G0386000
rosa_samantha Rh6AG500100 Rh6BG510800 Rh6CG516500 Rh6DG503000
rosa_wichuraiana Rw6G043560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 1 cut(s) 456
AciI CCGC 6 cut(s) 392, 454, 508, 833, 1298, 1315
AclWI GGATC 1 cut(s) 809
AcsI RAATTY 3 cut(s) 109, 679, 722
AcuI CTGAAG 3 cut(s) 192, 219, 873
AcvI CACGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 206
AfeI AGCGCT 1 cut(s) 761
AfiI CCNNNNNNNGG 5 cut(s) 70, 618, 863, 962, 1061
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 6 cut(s) 148, 328, 356, 473, 593, 616
AjnI CCWGG 2 cut(s) 309, 1024
AleI CACNNNNGTG 1 cut(s) 406
AluBI AGCT 9 cut(s) 100, 164, 236, 359, 482, 518, 536, 738, 773
AluI AGCT 9 cut(s) 100, 164, 236, 359, 482, 518, 536, 738, 773
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 1 cut(s) 779
AlwI GGATC 1 cut(s) 809
Aor51HI AGCGCT 1 cut(s) 761
AoxI GGCC 2 cut(s) 475, 1112
ApeKI GCWGC 4 cut(s) 164, 212, 275, 1160
ApoI RAATTY 3 cut(s) 109, 679, 722
Asp700I GAANNNNTTC 2 cut(s) 143, 722
AspLEI GCGC 2 cut(s) 507, 762
AsuHPI GGTGA 3 cut(s) 34, 230, 922
AsuII TTCGAA 2 cut(s) 677, 881
BanII GRGCYC 3 cut(s) 238, 451, 995
BbrPI CACGTG 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 123, 379
Bbv12I GWGCWC 1 cut(s) 238
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 151, 199, 262, 1172
BccI CCATC 4 cut(s) 738, 790, 826, 1285
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 311, 1026
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 4 cut(s) 243, 422, 789, 936
BfmI CTRYAG 2 cut(s) 222, 567
BfoI RGCGCY 2 cut(s) 508, 763
BisI GCNGC 8 cut(s) 165, 213, 276, 393, 454, 508, 1161, 1298
BlpI GCTNAGC 1 cut(s) 774
BlsI GCNGC 8 cut(s) 166, 214, 277, 394, 455, 509, 1162, 1299
Bme1390I CCNGG 2 cut(s) 311, 1026
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 2 cut(s) 311, 1026
BmsI GCATC 2 cut(s) 696, 876
BpiI GAAGAC 2 cut(s) 123, 379
Bpu10I CCTNAGC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 774
Bpu14I TTCGAA 2 cut(s) 677, 881
BpuEI CTTGAG 2 cut(s) 498, 1202
BsaAI YACGTR 1 cut(s) 440
BsaJI CCNNGG 5 cut(s) 301, 779, 961, 1024, 1197
BsaWI WCCGGW 1 cut(s) 1070
BsaXI ACNNNNNCTCC 4 cut(s) 776, 806, 820, 850
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 618, 863, 962, 1061
Bse1I ACTGG 1 cut(s) 1080
Bse3DI GCAATG 1 cut(s) 662
BseBI CCWGG 2 cut(s) 311, 1026
BseDI CCNNGG 5 cut(s) 301, 779, 961, 1024, 1197
BseGI GGATG 4 cut(s) 749, 771, 1043, 1207
BseLI CCNNNNNNNGG 5 cut(s) 70, 618, 863, 962, 1061
BseMI GCAATG 1 cut(s) 662
BseMII CTCAG 6 cut(s) 395, 528, 711, 813, 918, 1125
BseNI ACTGG 1 cut(s) 1080
BseXI GCAGC 4 cut(s) 151, 199, 262, 1172
BseYI CCCAGC 1 cut(s) 738
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 477, 1114
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 1071
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 5 cut(s) 70, 618, 863, 962, 1061
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 13
BsmI GAATGC 1 cut(s) 1232
BsnI GGCC 2 cut(s) 477, 1114
Bsp119I TTCGAA 2 cut(s) 677, 881
Bsp1286I GDGCHC 3 cut(s) 238, 451, 995
Bsp143I GATC 3 cut(s) 520, 662, 814
Bsp1720I GCTNAGC 1 cut(s) 774
Bsp19I CCATGG 2 cut(s) 779, 1197
BspACI CCGC 6 cut(s) 392, 454, 508, 833, 1298, 1315
BspANI GGCC 2 cut(s) 477, 1114
BspCNI CTCAG 6 cut(s) 396, 529, 710, 814, 917, 1126
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 610
BspPI GGATC 1 cut(s) 809
BspT104I TTCGAA 2 cut(s) 677, 881
BsrDI GCAATG 1 cut(s) 662
BsrI ACTGG 1 cut(s) 1080
BssECI CCNNGG 5 cut(s) 301, 779, 961, 1024, 1197
BssMI GATC 3 cut(s) 520, 662, 814
BssT1I CCWWGG 3 cut(s) 779, 961, 1197
Bst2UI CCWGG 2 cut(s) 311, 1026
Bst4CI ACNGT 2 cut(s) 226, 812
BstAPI GCANNNNNTGC 1 cut(s) 493
BstBAI YACGTR 1 cut(s) 440
BstBI TTCGAA 2 cut(s) 677, 881
BstC8I GCNNGC 6 cut(s) 390, 413, 417, 451, 458, 1307
BstDEI CTNAG 8 cut(s) 404, 537, 697, 774, 822, 904, 1134, 1143
BstDSI CCRYGG 2 cut(s) 779, 1197
BstENI CCTNNNNNAGG 2 cut(s) 616, 1059
BstF5I GGATG 4 cut(s) 749, 771, 1043, 1207
BstFNI CGCG 1 cut(s) 456
BstH2I RGCGCY 2 cut(s) 508, 763
BstHHI GCGC 2 cut(s) 507, 762
BstKTI GATC 3 cut(s) 523, 665, 817
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 3 cut(s) 520, 662, 814
BstMWI GCNNNNNNNGC 7 cut(s) 209, 218, 320, 329, 493, 502, 1306
BstNI CCWGG 2 cut(s) 311, 1026
BstNSI RCATGY 3 cut(s) 415, 496, 1309
BstSCI CCNGG 2 cut(s) 309, 1024
BstSFI CTRYAG 2 cut(s) 222, 567
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 4 cut(s) 151, 199, 262, 1172
BstV2I GAAGAC 2 cut(s) 123, 379
BsuRI GGCC 2 cut(s) 477, 1114
BtgI CCRYGG 2 cut(s) 779, 1197
BtsCI GGATG 4 cut(s) 749, 771, 1043, 1207
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 4 cut(s) 207, 231, 401, 817
Cac8I GCNNGC 6 cut(s) 390, 413, 417, 451, 458, 1307
CfoI GCGC 2 cut(s) 507, 762
Csp6I GTAC 1 cut(s) 205
CviAII CATG 7 cut(s) 412, 493, 780, 900, 1123, 1198, 1306
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 8 cut(s) 404, 537, 697, 774, 822, 904, 1134, 1143
DpnI GATC 3 cut(s) 522, 664, 816
DpnII GATC 3 cut(s) 520, 662, 814
Ecl136II GAGCTC 1 cut(s) 236
Eco130I CCWWGG 3 cut(s) 779, 961, 1197
Eco24I GRGCYC 3 cut(s) 238, 451, 995
Eco32I GATATC 1 cut(s) 942
Eco47III AGCGCT 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 236
Eco57I CTGAAG 3 cut(s) 192, 219, 873
Eco72I CACGTG 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 236
EcoNI CCTNNNNNAGG 2 cut(s) 616, 1059
EcoRI GAATTC 3 cut(s) 109, 679, 722
EcoRII CCWGG 2 cut(s) 309, 1024
EcoRV GATATC 1 cut(s) 942
EcoT14I CCWWGG 3 cut(s) 779, 961, 1197
EcoT38I GRGCYC 3 cut(s) 238, 451, 995
ErhI CCWWGG 3 cut(s) 779, 961, 1197
FaeI CATG 7 cut(s) 415, 496, 783, 903, 1126, 1201, 1309
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 1 cut(s) 13
FatI CATG 7 cut(s) 411, 492, 779, 899, 1122, 1197, 1305
FauI CCCGC 1 cut(s) 1322
Fnu4HI GCNGC 8 cut(s) 165, 213, 276, 393, 454, 508, 1161, 1298
FokI GGATG 4 cut(s) 756, 778, 1030, 1214
FriOI GRGCYC 3 cut(s) 238, 451, 995
Fsp4HI GCNGC 8 cut(s) 165, 213, 276, 393, 454, 508, 1161, 1298
FspBI CTAG 4 cut(s) 243, 422, 789, 936
GlaI GCGC 2 cut(s) 506, 761
GluI GCNGC 8 cut(s) 165, 213, 276, 393, 454, 508, 1161, 1298
GsaI CCCAGC 1 cut(s) 742
HaeII RGCGCY 2 cut(s) 508, 763
HaeIII GGCC 2 cut(s) 477, 1114
HapII CCGG 1 cut(s) 1071
HhaI GCGC 2 cut(s) 507, 762
Hin1II CATG 7 cut(s) 415, 496, 783, 903, 1126, 1201, 1309
Hin6I GCGC 2 cut(s) 505, 760
HinP1I GCGC 2 cut(s) 505, 760
HincII GTYRAC 1 cut(s) 1140
HindII GTYRAC 1 cut(s) 1140
HinfI GANTC 8 cut(s) 144, 154, 182, 285, 589, 674, 1136, 1247
HpaII CCGG 1 cut(s) 1071
HphI GGTGA 3 cut(s) 34, 230, 922
Hpy166II GTNNAC 4 cut(s) 207, 703, 828, 1140
Hpy188III TCNNGA 2 cut(s) 1062, 1219
Hpy8I GTNNAC 4 cut(s) 207, 703, 828, 1140
Hpy99I CGWCG 1 cut(s) 991
HpyAV CCTTC 8 cut(s) 167, 186, 322, 467, 536, 599, 622, 912
HpyCH4III ACNGT 2 cut(s) 226, 812
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 4 cut(s) 411, 415, 487, 851
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 218, 320, 329, 493, 502, 1306
HpyF3I CTNAG 8 cut(s) 404, 537, 697, 774, 822, 904, 1134, 1143
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 7 cut(s) 415, 496, 783, 903, 1126, 1201, 1309
HspAI GCGC 2 cut(s) 505, 760
Kzo9I GATC 3 cut(s) 520, 662, 814
LmnI GCTCC 1 cut(s) 608
Lsp1109I GCAGC 4 cut(s) 151, 199, 262, 1172
LweI GCATC 2 cut(s) 696, 876
MaeI CTAG 4 cut(s) 243, 422, 789, 936
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 6 cut(s) 155, 397, 928, 970, 1103, 1118
MalI GATC 3 cut(s) 522, 664, 816
MboI GATC 3 cut(s) 520, 662, 814
MboII GAAGA 5 cut(s) 94, 123, 384, 879, 1026
MfeI CAATTG 1 cut(s) 630
MhlI GDGCHC 3 cut(s) 238, 451, 995
MluCI AATT 7 cut(s) 109, 630, 679, 691, 722, 846, 1337
MlyI GAGTC 2 cut(s) 163, 1145
MmeI TCCRAC 1 cut(s) 831
MroXI GAANNNNTTC 2 cut(s) 143, 722
MseI TTAA 3 cut(s) 527, 579, 1340
MslI CAYNNNNRTG 1 cut(s) 406
MspA1I CMGCKG 1 cut(s) 738
MspI CCGG 1 cut(s) 1071
MspR9I CCNGG 2 cut(s) 311, 1026
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 1232
MvaI CCWGG 2 cut(s) 311, 1026
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 7 cut(s) 209, 218, 320, 329, 493, 502, 1306
NcoI CCATGG 2 cut(s) 779, 1197
NdeII GATC 3 cut(s) 520, 662, 814
NlaIII CATG 7 cut(s) 415, 496, 783, 903, 1126, 1201, 1309
NlaIV GGNNCC 1 cut(s) 610
NmuCI GTSAC 3 cut(s) 155, 928, 970
NspI RCATGY 3 cut(s) 415, 496, 1309
NspV TTCGAA 2 cut(s) 677, 881
OliI CACNNNNGTG 1 cut(s) 406
PaeI GCATGC 2 cut(s) 415, 1309
PcsI WCGNNNNNNNCGW 1 cut(s) 457
PctI GAATGC 1 cut(s) 1232
PdmI GAANNNNTTC 2 cut(s) 143, 722
PfeI GAWTC 6 cut(s) 144, 182, 285, 589, 674, 1247
PflFI GACNNNGTC 1 cut(s) 1135
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 8 cut(s) 166, 214, 277, 394, 455, 509, 1162, 1299
PleI GAGTC 2 cut(s) 162, 1144
PmaCI CACGTG 1 cut(s) 440
PmlI CACGTG 1 cut(s) 440
PpsI GAGTC 2 cut(s) 162, 1144
Ppu21I YACGTR 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 309, 1024
PspCI CACGTG 1 cut(s) 440
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 2 cut(s) 309, 1024
PspN4I GGNNCC 1 cut(s) 610
PsrI GAACNNNNNNTAC 2 cut(s) 1249, 1281
PsyI GACNNNGTC 1 cut(s) 1135
PvuII CAGCTG 1 cut(s) 738
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 406
SacI GAGCTC 1 cut(s) 238
SaqAI TTAA 3 cut(s) 527, 579, 1340
SatI GCNGC 8 cut(s) 165, 213, 276, 393, 454, 508, 1161, 1298
Sau3AI GATC 3 cut(s) 520, 662, 814
SchI GAGTC 2 cut(s) 163, 1145
ScrFI CCNGG 2 cut(s) 311, 1026
SduI GDGCHC 3 cut(s) 238, 451, 995
SfaNI GCATC 2 cut(s) 696, 876
SfcI CTRYAG 2 cut(s) 222, 567
SfuI TTCGAA 2 cut(s) 677, 881
SmiMI CAYNNNNRTG 1 cut(s) 406
SmlI CTYRAG 2 cut(s) 513, 1217
SmoI CTYRAG 2 cut(s) 513, 1217
SphI GCATGC 2 cut(s) 415, 1309
Sse9I AATT 7 cut(s) 109, 630, 679, 691, 722, 846, 1337
SsiI CCGC 6 cut(s) 392, 454, 508, 833, 1298, 1315
SspMI CTAG 4 cut(s) 243, 422, 789, 936
SstI GAGCTC 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 309, 1024
StyI CCWWGG 3 cut(s) 779, 961, 1197
TaaI ACNGT 2 cut(s) 226, 812
TaiI ACGT 1 cut(s) 442
TaqI TCGA 6 cut(s) 232, 427, 677, 726, 881, 989
TasI AATT 7 cut(s) 109, 630, 679, 691, 722, 846, 1337
TauI GCSGC 4 cut(s) 395, 456, 510, 1300
TfiI GAWTC 6 cut(s) 144, 182, 285, 589, 674, 1247
Tru1I TTAA 3 cut(s) 527, 579, 1340
Tru9I TTAA 3 cut(s) 527, 579, 1340
TscAI CASTG 4 cut(s) 214, 231, 408, 817
TseFI GTSAC 3 cut(s) 155, 928, 970
TseI GCWGC 4 cut(s) 164, 212, 275, 1160
Tsp45I GTSAC 3 cut(s) 155, 928, 970
TspDTI ATGAA 1 cut(s) 1139
TspRI CASTG 4 cut(s) 214, 231, 408, 817
Tth111I GACNNNGTC 1 cut(s) 1135
Van91I CCANNNNNTGG 1 cut(s) 962
XagI CCTNNNNNAGG 2 cut(s) 616, 1059
XapI RAATTY 3 cut(s) 109, 679, 722
XceI RCATGY 3 cut(s) 415, 496, 1309
XmnI GAANNNNTTC 2 cut(s) 143, 722
XspI CTAG 4 cut(s) 243, 422, 789, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.