FvH4_2g34260

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013469
Physical Location & Seq
Forward (+)
25496619 .. 25498536
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34260.t1

Sequence Viewer

Length: 1161 bp
ATGGCTTTGATGATAGACAACTGCGGCGAAGGCATACTCCTCTCTCTCGACTCCCACAAATCTGTCCCGGCTCCGTTTCTCACCAAAACGTACCAGCTCGTCGACGATCCCGTCACCGACCACATCGTCTCTTGGGGCGAAGACGACACCACCTTCGTCGTCTGGCGTCCTCCGGAGTTCGCCCGCGACCTCCTCCCCAACTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGTTTCAGAAAGATCGTGCCGGACAGGTGGGAGTTCGCCAACGAGTTTTTCAAGAAAGGTGAGAAGCATTTGCTCTGCGAGATCCACCGCCGCAAGACGGCTCAGCCGACACAGCTCCCCATCAACCACCATCAGTACCACCCGCATTCGCCGCTCGGCAGCCACCACGTCGTCACCGGTCCCGGATTCTTCCCCTTCCCACGTGGCAGCAACTCGCCTTCCGACTCCGACGACTTTCCCAACTGGTGTGACGACTCGCCGCCGCCACTCGTGTCATCTCCACCTTCCGGCTGCGGTGGTGGTCCCCTCAACACTGGCTACTGCAGTAACGTTGCGACCACTACCACCGTGACAGCTCTGTCGGAGGACAACGAGAGGCTGAGGCGGAGCAACACCATGCTGATGTCGGAGCTCAACCATATGAGGAAGCTTTACAACGATATCATCTACTTCGTTCAGAACCATGTCAAGCCTGTCGCTCCCAGCAATTCTTACCCAGGTTCTCTACTTCTCTGCAACCCAACACAAAACACACTCAACCCCAACAACTCATCCGCCGCCATCCACAAGCCTCTCAACCAGGTTTTTGGGTACCACAATAACCCTAAGCAAACCCAACCACATCATCAGCCAATGGCCCATGTCATGATGAACAACAACGTTAACGTCTCGTCTCCGACCACCGCCAAGAGTACTAGCTCGGTGACGATTATCGAAGACCGGCCCGGCAACAACAATGGGTGCAAGACCAAACTGTTTGGGGTTCCACTGCTGCATTCGAAGAAGAGACTGCACCCGGAGTACAACAACGGCTCGAACCACACAGCCATGGAAGCAACTAAAGCTCGCTTGATTTTGGAGAAGGATCATGATTTAGGTCTTCACCTTATGCCCCCATCCACATGTTAG

Protein Analysis

387

Amino Acids

43.21

Weight (kDa)

7.73

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 3.1e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46264
fragaria_vesca FvH4_2g34260
malus_domestica MD08G1098200.v1.1 MD15G1080700.v1.1
prunus_persica Prupe.1G433500_v2.0.a1
pyrus_communis pycom08g08000 pycom15g07520
rosa_chinensis RchiOBHm_Chr6g0308161
rosa_laevigata RLG00000010664
rosa_multiflora Rmu_sc0005706.1_g000009
rosa_roxburghii Rroxscaffold_7G00160400 Rroxscaffold_7G00160490
rosa_rugosa Rorug06G0364300
rosa_samantha Rh6AG473500 Rh6BG484300 Rh6CG489300 Rh6DG475500
rosa_wichuraiana Rw6G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 110, 125, 610
Acc65I GGTACC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 843
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 186
AccIII TCCGGA 1 cut(s) 172
AclI AACGTT 2 cut(s) 582, 912
AclWI GGATC 3 cut(s) 101, 328, 1125
AcvI CACGTG 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 5 cut(s) 92, 389, 845, 946, 1055
AfiI CCNNNNNNNGG 2 cut(s) 349, 539
AflIII ACRYGT 1 cut(s) 1154
AgeI ACCGGT 1 cut(s) 428
AgsI TTSAA 2 cut(s) 208, 304
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 748, 831
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 9 cut(s) 97, 228, 241, 367, 608, 664, 682, 951, 1097
AluI AGCT 9 cut(s) 97, 228, 241, 367, 608, 664, 682, 951, 1097
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 4 cut(s) 133, 925, 930, 1033
AlwI GGATC 3 cut(s) 101, 328, 1125
Aor13HI TCCGGA 1 cut(s) 172
AoxI GGCC 2 cut(s) 888, 974
ApeKI GCWGC 4 cut(s) 411, 459, 543, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
AsiGI ACCGGT 1 cut(s) 428
Asp718I GGTACC 1 cut(s) 843
AspS9I GGNCC 4 cut(s) 431, 554, 889, 975
AsuC2I CCSGG 4 cut(s) 68, 435, 978, 1049
AsuHPI GGTGA 6 cut(s) 73, 106, 323, 418, 967, 1127
AsuII TTCGAA 1 cut(s) 1031
AvaII GGWCC 2 cut(s) 431, 554
BanI GGYRCC 1 cut(s) 843
BanII GRGCYC 1 cut(s) 666
BauI CACGAG 1 cut(s) 521
BbrPI CACGTG 1 cut(s) 455
BbsI GAAGAC 3 cut(s) 147, 975, 1124
Bbv12I GWGCWC 1 cut(s) 666
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 4 cut(s) 423, 471, 530, 1011
BccI CCATC 4 cut(s) 380, 390, 821, 1156
BceAI ACGGC 2 cut(s) 366, 1078
BciT130I CCWGG 2 cut(s) 750, 833
BcnI CCSGG 4 cut(s) 68, 435, 978, 1049
BcoDI GTCTC 4 cut(s) 133, 925, 930, 1033
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 1 cut(s) 574
BlpI GCTNAGC 1 cut(s) 354
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 6 cut(s) 68, 435, 750, 833, 978, 1049
Bme18I GGWCC 2 cut(s) 431, 554
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 4 cut(s) 431, 554, 889, 975
BmiI GGNNCC 5 cut(s) 72, 433, 556, 845, 1017
BmrFI CCNGG 6 cut(s) 68, 435, 750, 833, 978, 1049
BpiI GAAGAC 3 cut(s) 147, 975, 1124
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 2 cut(s) 632, 858
Bpu1102I GCTNAGC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 1031
BpuMI CCSGG 4 cut(s) 68, 435, 978, 1049
BsaAI YACGTR 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 748, 1080
BsaWI WCCGGW 2 cut(s) 172, 428
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 539
Bse118I RCCGGY 2 cut(s) 428, 972
Bse1I ACTGG 2 cut(s) 500, 571
BseAI TCCGGA 1 cut(s) 172
BseBI CCWGG 2 cut(s) 750, 833
BseDI CCNNGG 2 cut(s) 748, 1080
BseGI GGATG 3 cut(s) 803, 813, 1148
BseLI CCNNNNNNNGG 2 cut(s) 349, 539
BseMII CTCAG 2 cut(s) 368, 623
BseNI ACTGG 2 cut(s) 500, 571
BseRI GAGGAG 2 cut(s) 29, 182
BseXI GCAGC 4 cut(s) 423, 471, 530, 1011
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 1 cut(s) 1028
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 890, 976
BshNI GGYRCC 1 cut(s) 843
BshTI ACCGGT 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 9 cut(s) 68, 173, 272, 429, 435, 540, 973, 978, 1049
BslFI GGGAC 3 cut(s) 50, 417, 540
BslI CCNNNNNNNGG 2 cut(s) 349, 539
BsmAI GTCTC 4 cut(s) 133, 925, 930, 1033
BsmBI CGTCTC 3 cut(s) 133, 925, 930
BsmFI GGGAC 3 cut(s) 50, 417, 540
BsmI GAATGC 2 cut(s) 397, 1027
BsnI GGCC 2 cut(s) 890, 976
Bsp119I TTCGAA 1 cut(s) 1031
Bsp1286I GDGCHC 1 cut(s) 666
Bsp13I TCCGGA 1 cut(s) 172
Bsp143I GATC 4 cut(s) 106, 264, 333, 1117
Bsp1720I GCTNAGC 1 cut(s) 354
Bsp19I CCATGG 1 cut(s) 1080
BspANI GGCC 2 cut(s) 890, 976
BspCNI CTCAG 2 cut(s) 367, 624
BspEI TCCGGA 1 cut(s) 172
BspFNI CGCG 1 cut(s) 186
BspHI TCATGA 2 cut(s) 897, 1120
BspLI GGNNCC 5 cut(s) 72, 433, 556, 845, 1017
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 3 cut(s) 101, 328, 1125
BspT104I TTCGAA 1 cut(s) 1031
BspT107I GGYRCC 1 cut(s) 843
BsrBI CCGCTC 1 cut(s) 406
BsrFI RCCGGY 2 cut(s) 428, 972
BsrI ACTGG 2 cut(s) 500, 571
BssAI RCCGGY 2 cut(s) 428, 972
BssECI CCNNGG 2 cut(s) 748, 1080
BssMI GATC 4 cut(s) 106, 264, 333, 1117
BssNI GRCGYC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 1080
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 750, 833
Bst4CI ACNGT 2 cut(s) 601, 1008
Bst6I CTCTTC 1 cut(s) 1031
BstACI GRCGYC 1 cut(s) 166
BstBAI YACGTR 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 1031
BstC8I GCNNGC 3 cut(s) 184, 239, 1099
BstDEI CTNAG 3 cut(s) 354, 632, 858
BstDSI CCRYGG 1 cut(s) 1080
BstF5I GGATG 3 cut(s) 803, 813, 1148
BstFNI CGCG 1 cut(s) 186
BstKTI GATC 4 cut(s) 109, 267, 336, 1120
BstMAI GTCTC 4 cut(s) 133, 925, 930, 1033
BstMBI GATC 4 cut(s) 106, 264, 333, 1117
BstMWI GCNNNNNNNGC 6 cut(s) 30, 234, 364, 403, 1085, 1094
BstNI CCWGG 2 cut(s) 750, 833
BstNSI RCATGY 1 cut(s) 1158
BstSCI CCNGG 6 cut(s) 66, 433, 748, 831, 976, 1047
BstSFI CTRYAG 1 cut(s) 574
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 4 cut(s) 423, 471, 530, 1011
BstV2I GAAGAC 3 cut(s) 147, 975, 1124
BstX2I RGATCY 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 2 cut(s) 890, 976
BtgI CCRYGG 1 cut(s) 1080
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 3 cut(s) 803, 813, 1148
BtsI GCAGTG 1 cut(s) 1019
BtsIMutI CAGTG 2 cut(s) 564, 1019
Cac8I GCNNGC 3 cut(s) 184, 239, 1099
CciI TCATGA 2 cut(s) 897, 1120
Cfr10I RCCGGY 2 cut(s) 428, 972
Cfr13I GGNCC 4 cut(s) 431, 554, 889, 975
CseI GACGC 1 cut(s) 155
CsiI ACCWGGT 1 cut(s) 831
Csp6I GTAC 5 cut(s) 91, 388, 844, 945, 1054
CspAI ACCGGT 1 cut(s) 428
CviAII CATG 7 cut(s) 649, 716, 893, 898, 1081, 1121, 1155
CviQI GTAC 5 cut(s) 91, 388, 844, 945, 1054
DdeI CTNAG 3 cut(s) 354, 632, 858
DpnI GATC 4 cut(s) 108, 266, 335, 1119
DpnII GATC 4 cut(s) 106, 264, 333, 1117
DrdI GACNNNNNNGTC 3 cut(s) 110, 125, 610
DseDI GACNNNNNNGTC 3 cut(s) 110, 125, 610
Eam1104I CTCTTC 1 cut(s) 1031
EarI CTCTTC 1 cut(s) 1031
EciI GGCGGA 3 cut(s) 224, 652, 796
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 1080
Eco24I GRGCYC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 2 cut(s) 431, 554
Eco53kI GAGCTC 1 cut(s) 664
Eco72I CACGTG 1 cut(s) 455
EcoICRI GAGCTC 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 748, 831
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 1080
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 1080
Esp3I CGTCTC 3 cut(s) 133, 925, 930
FaeI CATG 7 cut(s) 652, 719, 896, 901, 1084, 1124, 1158
FaqI GGGAC 3 cut(s) 50, 417, 540
FatI CATG 7 cut(s) 648, 715, 892, 897, 1080, 1120, 1154
FauI CCCGC 2 cut(s) 191, 402
FauNDI CATATG 1 cut(s) 672
FblI GTMKAC 1 cut(s) 102
FokI GGATG 3 cut(s) 790, 800, 1135
FriOI GRGCYC 1 cut(s) 666
FspBI CTAG 1 cut(s) 948
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 2 cut(s) 890, 976
HapII CCGG 9 cut(s) 68, 173, 272, 429, 435, 540, 973, 978, 1049
HgaI GACGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 7 cut(s) 652, 719, 896, 901, 1084, 1124, 1158
HincII GTYRAC 2 cut(s) 103, 916
HindII GTYRAC 2 cut(s) 103, 916
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 4 cut(s) 50, 438, 476, 506
HpaI GTTAAC 1 cut(s) 916
HpaII CCGG 9 cut(s) 68, 173, 272, 429, 435, 540, 973, 978, 1049
HphI GGTGA 6 cut(s) 73, 106, 323, 418, 967, 1127
Hpy166II GTNNAC 2 cut(s) 103, 916
Hpy188I TCNGA 7 cut(s) 260, 475, 481, 616, 661, 711, 930
Hpy188III TCNNGA 5 cut(s) 47, 173, 304, 898, 1121
Hpy8I GTNNAC 2 cut(s) 103, 916
Hpy99I CGWCG 5 cut(s) 104, 107, 161, 425, 485
HpyAV CCTTC 6 cut(s) 23, 163, 457, 480, 546, 1108
HpyCH4III ACNGT 2 cut(s) 601, 1008
HpyCH4IV ACGT 6 cut(s) 89, 420, 454, 582, 912, 918
HpyCH4V TGCA 5 cut(s) 576, 768, 996, 1027, 1045
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 234, 364, 403, 1085, 1094
HpyF3I CTNAG 3 cut(s) 354, 632, 858
HpySE526I ACGT 6 cut(s) 89, 420, 454, 582, 912, 918
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 7 cut(s) 652, 719, 896, 901, 1084, 1124, 1158
Kpn2I TCCGGA 1 cut(s) 172
KpnI GGTACC 1 cut(s) 847
KspAI GTTAAC 1 cut(s) 916
Kzo9I GATC 4 cut(s) 106, 264, 333, 1117
LmnI GCTCC 5 cut(s) 76, 372, 639, 661, 736
Lsp1109I GCAGC 4 cut(s) 423, 471, 530, 1011
MabI ACCWGGT 1 cut(s) 831
MaeI CTAG 1 cut(s) 948
MaeII ACGT 6 cut(s) 89, 420, 454, 582, 912, 918
MaeIII GTNAC 6 cut(s) 112, 424, 500, 578, 601, 955
MalI GATC 4 cut(s) 108, 266, 335, 1119
MbiI CCGCTC 1 cut(s) 406
MboI GATC 4 cut(s) 106, 264, 333, 1117
MboII GAAGA 6 cut(s) 152, 433, 980, 1045, 1048, 1124
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 1 cut(s) 739
MlyI GAGTC 3 cut(s) 44, 470, 500
MmeI TCCRAC 5 cut(s) 498, 504, 594, 639, 953
MroI TCCGGA 1 cut(s) 172
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 3 cut(s) 653, 1079, 1153
MspI CCGG 9 cut(s) 68, 173, 272, 429, 435, 540, 973, 978, 1049
MspR9I CCNGG 6 cut(s) 68, 435, 750, 833, 978, 1049
Mva1269I GAATGC 2 cut(s) 397, 1027
MvaI CCWGG 2 cut(s) 750, 833
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 6 cut(s) 30, 234, 364, 403, 1085, 1094
NciI CCSGG 4 cut(s) 68, 435, 978, 1049
NcoI CCATGG 1 cut(s) 1080
NdeI CATATG 1 cut(s) 672
NdeII GATC 4 cut(s) 106, 264, 333, 1117
NlaIII CATG 7 cut(s) 652, 719, 896, 901, 1084, 1124, 1158
NlaIV GGNNCC 5 cut(s) 72, 433, 556, 845, 1017
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 5 cut(s) 112, 424, 500, 601, 955
NspI RCATGY 1 cut(s) 1158
NspV TTCGAA 1 cut(s) 1031
PagI TCATGA 2 cut(s) 897, 1120
PciI ACATGT 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 356
PctI GAATGC 2 cut(s) 397, 1027
PfeI GAWTC 1 cut(s) 438
PfoI TCCNGGA 1 cut(s) 433
PinAI ACCGGT 1 cut(s) 428
PleI GAGTC 3 cut(s) 44, 470, 500
PmaCI CACGTG 1 cut(s) 455
PmlI CACGTG 1 cut(s) 455
PpsI GAGTC 3 cut(s) 44, 470, 500
Ppu21I YACGTR 1 cut(s) 455
PscI ACATGT 1 cut(s) 1154
Psp124BI GAGCTC 1 cut(s) 666
Psp1406I AACGTT 2 cut(s) 582, 912
Psp6I CCWGG 2 cut(s) 748, 831
PspCI CACGTG 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 2 cut(s) 748, 831
PspN4I GGNNCC 5 cut(s) 72, 433, 556, 845, 1017
PspPI GGNCC 4 cut(s) 431, 554, 889, 975
PstI CTGCAG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 5 cut(s) 92, 389, 845, 946, 1055
RsaNI GTAC 5 cut(s) 91, 388, 844, 945, 1054
RseI CAYNNNNRTG 3 cut(s) 653, 1079, 1153
SacI GAGCTC 1 cut(s) 666
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 1 cut(s) 915
Sau3AI GATC 4 cut(s) 106, 264, 333, 1117
Sau96I GGNCC 4 cut(s) 431, 554, 889, 975
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 3 cut(s) 44, 470, 500
ScrFI CCNGG 6 cut(s) 68, 435, 750, 833, 978, 1049
SduI GDGCHC 1 cut(s) 666
SexAI ACCWGGT 1 cut(s) 831
SfcI CTRYAG 1 cut(s) 574
SfuI TTCGAA 1 cut(s) 1031
SgrDI CGTCGACG 1 cut(s) 101
SinI GGWCC 2 cut(s) 431, 554
SmiMI CAYNNNNRTG 3 cut(s) 653, 1079, 1153
Sse9I AATT 1 cut(s) 739
SspMI CTAG 1 cut(s) 948
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 6 cut(s) 66, 433, 748, 831, 976, 1047
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 601, 1008
TaiI ACGT 6 cut(s) 92, 423, 457, 585, 915, 921
TaqI TCGA 5 cut(s) 48, 102, 966, 1031, 1067
TasI AATT 1 cut(s) 739
TatI WGTACW 2 cut(s) 944, 1053
TauI GCSGC 6 cut(s) 27, 345, 406, 514, 517, 812
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 2 cut(s) 571, 1026
TseFI GTSAC 5 cut(s) 112, 424, 500, 601, 955
TseI GCWGC 4 cut(s) 411, 459, 543, 1024
Tsp45I GTSAC 5 cut(s) 112, 424, 500, 601, 955
TspDTI ATGAA 1 cut(s) 917
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 2 cut(s) 571, 1026
VpaK11BI GGWCC 2 cut(s) 431, 554
XceI RCATGY 1 cut(s) 1158
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 1 cut(s) 948
ZrmI AGTACT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.