MD08G1098200.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013469
Physical Location & Seq
Reverse (-)
8272110 .. 8273784
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1098200.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGGCGTTGATGATAGACAACTGCGAGAGCATACTACTCTCCCTGGATTCCCACAAGTCCGTCCCGGCGCCGTTCCTGACCAAAACCTACCAACTCGTCGACGACCCCGCCACCGACCACATCGTCTCGTGGGGCGACGACGACACCACCTTCGTCGTCTGGCGCCCTCCCGAGTTTGCCCGCGACCTCCTCCCCAACTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACCTACGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCCAACGAGTTCTTCAAAAAAGGAGAGAAGCATTTGCTTTGCGAGATCCACCGGAGAAAGACAGCTCAGCCACATCAGGTGGGTCTCAACCACCACCACCACCACCACTCCCCCCTCGGCATGAACGGCCACCACCATCCTAGCTTCTTCTCCTTCCCTAGTCGCGGCAGCATCTCGCCCTCCGACTCGGACGAGCAGCCCAACTGGTGCGAGTCGGACTCCCCTCCTCTCTTATCCCCAACCGGAGGTATTAACACAAACATTAACACTAACAACAATACCTTCATGAATATTAATCATAATAATACCACGGTGGCGGGTTTGGCGGAGGACAACGAGAGGCTGCGGAGGAGCAACAACATGCTAATGTCTGAGTTGACACACATGAGAAAACTCTACAACGACATCATCTACTTTGTCCAGAACCACGTCAAGCCGGTGGCTCCGAGCAACTCCTACCCTTCTTCCATGCTTCTCTGCAACCCACAGCCTCCTAAACCTAATGGTTCTAATGGTATTTTGAACCAACTTCAAGGGTACTATCCGGCTGCTCCTCCACCTAATGCCAAACAAAACCCTCATCACATCATGAACTCTTCCAGTCCGATGAGCAACACCACGTCGAAGAGCTCCGTGACGATTCTCGAAGATCACCACCAACAGCCGCCGAGCAGTAACAATGGGTGCAAGAACACTAAGCTTTTTGGGGTGCCGCTGCTTCACTCGAAGAAACGATTGCACCCGGAGGAATACGGCTCAAATAATCACCATGGGAACAACATGATGGAGGCCAGCAAGGCTCGTTTGATTTTGGAGAAGGATGATTTGGGTCTCCAGCTTATGCCTCCATCCACATGTTAA

Protein Analysis

382

Amino Acids

43.05

Weight (kDa)

7.15

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 25 - 114 1.2e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46264
fragaria_vesca FvH4_2g34260
malus_domestica MD08G1098200.v1.1 MD15G1080700.v1.1
prunus_persica Prupe.1G433500_v2.0.a1
pyrus_communis pycom08g08000 pycom15g07520
rosa_chinensis RchiOBHm_Chr6g0308161
rosa_laevigata RLG00000010664
rosa_multiflora Rmu_sc0005706.1_g000009
rosa_roxburghii Rroxscaffold_7G00160400 Rroxscaffold_7G00160490
rosa_rugosa Rorug06G0364300
rosa_samantha Rh6AG473500 Rh6BG484300 Rh6CG489300 Rh6DG475500
rosa_wichuraiana Rw6G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 3 cut(s) 67, 162, 994
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 183, 450
AciI CCGC 9 cut(s) 108, 181, 232, 450, 602, 611, 631, 950, 998
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 412
AcyI GRCGYC 2 cut(s) 68, 163
AfaI GTAC 2 cut(s) 267, 824
AfiI CCNNNNNNNGG 2 cut(s) 449, 530
AflIII ACRYGT 1 cut(s) 1139
AgsI TTSAA 4 cut(s) 205, 301, 808, 818
AjiI CACGTC 2 cut(s) 715, 906
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 1094, 1126
AluBI AGCT 7 cut(s) 225, 238, 350, 429, 915, 985, 1123
AluI AGCT 7 cut(s) 225, 238, 350, 429, 915, 985, 1123
Alw21I GWGCWC 1 cut(s) 917
Alw26I GTCTC 3 cut(s) 130, 374, 1121
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 412, 1074
ApeKI GCWGC 5 cut(s) 453, 481, 628, 833, 1000
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 1 cut(s) 579
AspLEI GCGC 2 cut(s) 70, 165
AsuC2I CCSGG 2 cut(s) 65, 1028
AsuHPI GGTGA 2 cut(s) 929, 1043
AvaI CYCGRG 1 cut(s) 170
BanI GGYRCC 3 cut(s) 67, 162, 994
BanII GRGCYC 1 cut(s) 917
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 917
BbvI GCAGC 5 cut(s) 465, 493, 615, 820, 987
BccI CCATC 4 cut(s) 270, 429, 1063, 1141
BceAI ACGGC 3 cut(s) 55, 427, 1054
BciT130I CCWGG 1 cut(s) 44
BcnI CCSGG 2 cut(s) 65, 1028
BcoDI GTCTC 3 cut(s) 130, 374, 1121
BfaI CTAG 2 cut(s) 426, 444
BfoI RGCGCY 2 cut(s) 71, 166
BglI GCCNNNNNGGC 1 cut(s) 1082
BisI GCNGC 8 cut(s) 451, 454, 482, 629, 834, 950, 998, 1001
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 8 cut(s) 452, 455, 483, 630, 835, 951, 999, 1002
Bme1390I CCNGG 3 cut(s) 44, 65, 1028
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 2 cut(s) 715, 906
BmiI GGNNCC 4 cut(s) 69, 164, 729, 996
BmrFI CCNGG 3 cut(s) 44, 65, 1028
BmsI GCATC 1 cut(s) 465
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpmI CTGGAG 2 cut(s) 205, 1103
Bpu1102I GCTNAGC 1 cut(s) 351
BpuMI CCSGG 2 cut(s) 65, 1028
BsaHI GRCGYC 2 cut(s) 68, 163
BsaI GGTCTC 2 cut(s) 374, 1121
BsaJI CCNNGG 4 cut(s) 42, 400, 594, 1054
BsaWI WCCGGW 3 cut(s) 268, 336, 527
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 377, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 530
Bse118I RCCGGY 1 cut(s) 721
Bse1I ACTGG 2 cut(s) 494, 885
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 4 cut(s) 42, 400, 594, 1054
BseGI GGATG 3 cut(s) 421, 1111, 1133
BseLI CCNNNNNNNGG 2 cut(s) 449, 530
BseMII CTCAG 2 cut(s) 365, 648
BseNI ACTGG 2 cut(s) 494, 885
BseRI GAGGAG 4 cut(s) 179, 501, 649, 828
BseXI GCAGC 5 cut(s) 465, 493, 615, 820, 987
Bsh1236I CGCG 2 cut(s) 183, 450
BshFI GGCC 2 cut(s) 414, 1076
BshNI GGYRCC 3 cut(s) 67, 162, 994
BsiHKAI GWGCWC 1 cut(s) 917
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 7 cut(s) 65, 269, 337, 528, 722, 830, 1028
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 449, 530
BsmAI GTCTC 3 cut(s) 130, 374, 1121
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 2 cut(s) 414, 1076
Bso31I GGTCTC 2 cut(s) 374, 1121
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 917
Bsp143I GATC 2 cut(s) 330, 934
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 1054
BspACI CCGC 9 cut(s) 108, 181, 232, 450, 602, 611, 631, 950, 998
BspANI GGCC 2 cut(s) 414, 1076
BspCNI CTCAG 2 cut(s) 364, 649
BspFNI CGCG 2 cut(s) 183, 450
BspHI TCATGA 2 cut(s) 570, 873
BspLI GGNNCC 4 cut(s) 69, 164, 729, 996
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 905
BspT107I GGYRCC 3 cut(s) 67, 162, 994
BspTNI GGTCTC 2 cut(s) 374, 1121
BsrFI RCCGGY 1 cut(s) 721
BsrI ACTGG 2 cut(s) 494, 885
BssAI RCCGGY 1 cut(s) 721
BssECI CCNNGG 4 cut(s) 42, 400, 594, 1054
BssMI GATC 2 cut(s) 330, 934
BssNI GRCGYC 2 cut(s) 68, 163
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 251, 598
Bst6I CTCTTC 2 cut(s) 886, 905
BstACI GRCGYC 2 cut(s) 68, 163
BstC8I GCNNGC 3 cut(s) 181, 236, 1078
BstDEI CTNAG 3 cut(s) 351, 657, 981
BstDSI CCRYGG 2 cut(s) 594, 1054
BstF5I GGATG 3 cut(s) 421, 1111, 1133
BstFNI CGCG 2 cut(s) 183, 450
BstH2I RGCGCY 2 cut(s) 71, 166
BstHHI GCGC 2 cut(s) 70, 165
BstKTI GATC 2 cut(s) 333, 937
BstMAI GTCTC 3 cut(s) 130, 374, 1121
BstMBI GATC 2 cut(s) 330, 934
BstMWI GCNNNNNNNGC 4 cut(s) 231, 411, 608, 1082
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 2 cut(s) 649, 1143
BstSCI CCNGG 3 cut(s) 42, 63, 1026
BstUI CGCG 2 cut(s) 183, 450
BstV1I GCAGC 5 cut(s) 465, 493, 615, 820, 987
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 2 cut(s) 414, 1076
BtgI CCRYGG 2 cut(s) 594, 1054
BtrI CACGTC 2 cut(s) 715, 906
BtsCI GGATG 3 cut(s) 421, 1111, 1133
Cac8I GCNNGC 3 cut(s) 181, 236, 1078
CciI TCATGA 2 cut(s) 570, 873
CfoI GCGC 2 cut(s) 70, 165
Cfr10I RCCGGY 1 cut(s) 721
Csp6I GTAC 2 cut(s) 266, 823
CviAII CATG 9 cut(s) 406, 571, 646, 670, 754, 874, 1055, 1066, 1140
CviQI GTAC 2 cut(s) 266, 823
DdeI CTNAG 3 cut(s) 351, 657, 981
DinI GGCGCC 2 cut(s) 69, 164
DpnI GATC 2 cut(s) 332, 936
DpnII GATC 2 cut(s) 330, 934
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 886, 905
EarI CTCTTC 2 cut(s) 886, 905
EciI GGCGGA 2 cut(s) 221, 626
Ecl136II GAGCTC 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 1054
Eco24I GRGCYC 1 cut(s) 917
Eco31I GGTCTC 2 cut(s) 374, 1121
Eco53kI GAGCTC 1 cut(s) 915
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 915
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 1054
EcoT38I GRGCYC 1 cut(s) 917
EgeI GGCGCC 2 cut(s) 69, 164
EheI GGCGCC 2 cut(s) 69, 164
ErhI CCWWGG 1 cut(s) 1054
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 9 cut(s) 409, 574, 649, 673, 757, 877, 1058, 1069, 1143
FaqI GGGAC 1 cut(s) 47
FatI CATG 9 cut(s) 405, 570, 645, 669, 753, 873, 1054, 1065, 1139
FauI CCCGC 3 cut(s) 115, 188, 595
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 8 cut(s) 451, 454, 482, 629, 834, 950, 998, 1001
FokI GGATG 3 cut(s) 408, 1118, 1120
FriOI GRGCYC 1 cut(s) 917
Fsp4HI GCNGC 8 cut(s) 451, 454, 482, 629, 834, 950, 998, 1001
FspBI CTAG 2 cut(s) 426, 444
GlaI GCGC 2 cut(s) 69, 164
GluI GCNGC 8 cut(s) 451, 454, 482, 629, 834, 950, 998, 1001
GsuI CTGGAG 2 cut(s) 205, 1103
HaeII RGCGCY 2 cut(s) 71, 166
HaeIII GGCC 2 cut(s) 414, 1076
HapII CCGG 7 cut(s) 65, 269, 337, 528, 722, 830, 1028
HhaI GCGC 2 cut(s) 70, 165
Hin1I GRCGYC 2 cut(s) 68, 163
Hin1II CATG 9 cut(s) 409, 574, 649, 673, 757, 877, 1058, 1069, 1143
Hin6I GCGC 2 cut(s) 68, 163
HinP1I GCGC 2 cut(s) 68, 163
HincII GTYRAC 2 cut(s) 100, 663
HindII GTYRAC 2 cut(s) 100, 663
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 5 cut(s) 47, 470, 497, 503, 925
HpaII CCGG 7 cut(s) 65, 269, 337, 528, 722, 830, 1028
HphI GGTGA 2 cut(s) 929, 1043
Hpy166II GTNNAC 2 cut(s) 100, 663
Hpy188I TCNGA 6 cut(s) 469, 475, 502, 658, 732, 891
Hpy188III TCNNGA 6 cut(s) 76, 170, 571, 706, 874, 929
Hpy8I GTNNAC 2 cut(s) 100, 663
Hpy99I CGWCG 6 cut(s) 101, 104, 140, 143, 158, 910
HpyAV CCTTC 5 cut(s) 160, 448, 577, 756, 1096
HpyCH4III ACNGT 2 cut(s) 251, 598
HpyCH4IV ACGT 2 cut(s) 714, 905
HpyCH4V TGCA 3 cut(s) 765, 972, 1024
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 411, 608, 1082
HpyF3I CTNAG 3 cut(s) 351, 657, 981
HpySE526I ACGT 2 cut(s) 714, 905
Hsp92I GRCGYC 2 cut(s) 68, 163
Hsp92II CATG 9 cut(s) 409, 574, 649, 673, 757, 877, 1058, 1069, 1143
HspAI GCGC 2 cut(s) 68, 163
KasI GGCGCC 2 cut(s) 67, 162
Kzo9I GATC 2 cut(s) 330, 934
LguI GCTCTTC 1 cut(s) 905
LmnI GCTCC 4 cut(s) 636, 733, 841, 920
Lsp1109I GCAGC 5 cut(s) 465, 493, 615, 820, 987
LweI GCATC 1 cut(s) 465
MaeI CTAG 2 cut(s) 426, 444
MaeII ACGT 2 cut(s) 714, 905
MaeIII GTNAC 2 cut(s) 919, 959
MalI GATC 2 cut(s) 332, 936
MboI GATC 2 cut(s) 330, 934
MboII GAAGA 8 cut(s) 271, 289, 424, 741, 873, 922, 944, 1024
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 917
Mly113I GGCGCC 2 cut(s) 68, 163
MlyI GAGTC 3 cut(s) 464, 497, 506
MmeI TCCRAC 2 cut(s) 480, 492
MseI TTAA 4 cut(s) 537, 549, 579, 1144
MslI CAYNNNNRTG 2 cut(s) 650, 1138
MspA1I CMGCKG 1 cut(s) 1000
MspI CCGG 7 cut(s) 65, 269, 337, 528, 722, 830, 1028
MspR9I CCNGG 3 cut(s) 44, 65, 1028
MvaI CCWGG 1 cut(s) 44
MvnI CGCG 2 cut(s) 183, 450
MwoI GCNNNNNNNGC 4 cut(s) 231, 411, 608, 1082
NarI GGCGCC 2 cut(s) 68, 163
NciI CCSGG 2 cut(s) 65, 1028
NcoI CCATGG 1 cut(s) 1054
NdeII GATC 2 cut(s) 330, 934
NlaIII CATG 9 cut(s) 409, 574, 649, 673, 757, 877, 1058, 1069, 1143
NlaIV GGNNCC 4 cut(s) 69, 164, 729, 996
NmeAIII GCCGAG 2 cut(s) 381, 978
NmuCI GTSAC 1 cut(s) 919
NspI RCATGY 2 cut(s) 649, 1143
PagI TCATGA 2 cut(s) 570, 873
PciI ACATGT 1 cut(s) 1139
PciSI GCTCTTC 1 cut(s) 905
PfeI GAWTC 2 cut(s) 47, 925
PkrI GCNGC 8 cut(s) 452, 455, 483, 630, 835, 951, 999, 1002
PleI GAGTC 3 cut(s) 464, 497, 505
PluTI GGCGCC 2 cut(s) 71, 166
PpsI GAGTC 3 cut(s) 464, 497, 505
PscI ACATGT 1 cut(s) 1139
PshBI ATTAAT 1 cut(s) 579
Psp124BI GAGCTC 1 cut(s) 917
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 4 cut(s) 69, 164, 729, 996
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 2 cut(s) 267, 824
RsaNI GTAC 2 cut(s) 266, 823
RseI CAYNNNNRTG 2 cut(s) 650, 1138
SacI GAGCTC 1 cut(s) 917
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 905
SaqAI TTAA 4 cut(s) 537, 549, 579, 1144
SatI GCNGC 8 cut(s) 451, 454, 482, 629, 834, 950, 998, 1001
Sau3AI GATC 2 cut(s) 330, 934
SchI GAGTC 3 cut(s) 464, 497, 506
ScrFI CCNGG 3 cut(s) 44, 65, 1028
SduI GDGCHC 1 cut(s) 917
SfaNI GCATC 1 cut(s) 465
SfoI GGCGCC 2 cut(s) 69, 164
SgrDI CGTCGACG 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 650, 1138
SsiI CCGC 9 cut(s) 108, 181, 232, 450, 602, 611, 631, 950, 998
SspDI GGCGCC 2 cut(s) 67, 162
SspI AATATT 1 cut(s) 577
SspMI CTAG 2 cut(s) 426, 444
SstI GAGCTC 1 cut(s) 917
StyD4I CCNGG 3 cut(s) 42, 63, 1026
StyI CCWWGG 1 cut(s) 1054
TaaI ACNGT 2 cut(s) 251, 598
TaiI ACGT 2 cut(s) 717, 908
TaqI TCGA 4 cut(s) 99, 908, 930, 1010
TauI GCSGC 3 cut(s) 453, 952, 1000
TfiI GAWTC 2 cut(s) 47, 925
Tru1I TTAA 4 cut(s) 537, 549, 579, 1144
Tru9I TTAA 4 cut(s) 537, 549, 579, 1144
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 5 cut(s) 453, 481, 628, 833, 1000
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 4 cut(s) 422, 559, 587, 890
TspGWI ACGGA 2 cut(s) 49, 907
VspI ATTAAT 1 cut(s) 579
XceI RCATGY 2 cut(s) 649, 1143
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 426, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.