MD15G1080700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013469
Physical Location & Seq
Reverse (-)
5510333 .. 5512142
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1080700.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGCGTTGATGATAGACAACTGCGAGGGCATACTGCTCTCTCTGGACTCCCACAAGTCGGTCCCGGCGCCGTTCCTGACCAAAACCTACCAGCTCGTCGACGACCCCGCCACCGACCACATCGTCTCGTGGGGCGACGACGACACCACCTTCGTTGTCTGGCGCCCTCCCGAGTTCGCCCGCGACCTCCTCCCCAACTACTTCAAGCACAACAACTTCTCAAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCGAACGAGTTCTTCAAAAAGGGAGAGAAGCATTTGCTTTGCGAGATCCACCGGAGAAAAACAGCTCAGCCACATCAGGTGGGTCTCAGCCACCACCACCACCACCACTCCCAACTCGGCATGAACGGCCACCACCACCATCCTGGCTTTTTCCCCTTCCCAAGTCCCGGCAGCATCTCGCCCTCCGACTCGGACGAGCAGCCCAACTGGTGCGATTCGGACTCCCCTCCTCTCTTATCCCCAACGGGAGGTATTAACACAAACATTAACTCTAACAACAATAATTTCATGAATATTAACAATAATAATACCACGGTGGCGGGTTTGGCGGAGGACAACGAGAGGCTGCGGAGGAGCAACACCATGCTAATGTCTGAGCTGGCACATATGAGAAAACTCTACAACGACATCATCTACTTTGTTCAGAACCATGTCAAGCCGGTGGCTCCGAGCAACTCATACCCTTCTTCCATGCTTCTCTGCAACCCACAGCCTCCTAAACATAATGGTCCTAATAGTAATTTGAACCAACTTCTAGGGTACTATCCGGCTGCTCCTCCACCTAATGCCAAACAAAACCCTCATCATATCATGAACTCTTCCAGTCCGATGAGCAACACCACGTCGAAGAGCTCCTCCGTCACTATTCTCGAAGACCATCACCAGCAGCCTCCGAGCGGTAACAATGGGTGCAAGAACACTAAACTTTTTGGGGTGCCGCTGCTTCATTCGAAGAAACGATTGCACCCGGAGGAGTACGGCTCGAATAATCACCATGGGAACAACATGATGGAGGCCAGCAAGGCTCGTTTGATTTTGGAGAAGGATGATTTAGGACTCCACCTCATGCCTCCATCCAGATGTTAG

Protein Analysis

384

Amino Acids

43.19

Weight (kDa)

7.17

Isoelectric Point (pI)

53.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 25 - 114 1.2e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46264
fragaria_vesca FvH4_2g34260
malus_domestica MD08G1098200.v1.1 MD15G1080700.v1.1
prunus_persica Prupe.1G433500_v2.0.a1
pyrus_communis pycom08g08000 pycom15g07520
rosa_chinensis RchiOBHm_Chr6g0308161
rosa_laevigata RLG00000010664
rosa_multiflora Rmu_sc0005706.1_g000009
rosa_roxburghii Rroxscaffold_7G00160400 Rroxscaffold_7G00160490
rosa_rugosa Rorug06G0364300
rosa_samantha Rh6AG473500 Rh6BG484300 Rh6CG489300 Rh6DG475500
rosa_wichuraiana Rw6G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 3 cut(s) 67, 162, 1000
AccBSI CCGCTC 1 cut(s) 963
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 183
AciI CCGC 8 cut(s) 108, 181, 232, 605, 614, 634, 963, 1004
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 412
AcyI GRCGYC 2 cut(s) 68, 163
AfaI GTAC 3 cut(s) 267, 827, 1043
AfiI CCNNNNNNNGG 4 cut(s) 58, 452, 533, 962
AgsI TTSAA 3 cut(s) 205, 301, 811
AjiI CACGTC 1 cut(s) 909
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 6 cut(s) 94, 225, 238, 350, 664, 918
AluI AGCT 6 cut(s) 94, 225, 238, 350, 664, 918
Alw21I GWGCWC 1 cut(s) 920
Alw26I GTCTC 2 cut(s) 130, 374
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 412, 1080
ApeKI GCWGC 6 cut(s) 456, 484, 631, 836, 952, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 2 cut(s) 70, 165
AspS9I GGNCC 2 cut(s) 61, 794
AsuC2I CCSGG 3 cut(s) 65, 453, 1034
AsuHPI GGTGA 2 cut(s) 938, 1049
AsuII TTCGAA 1 cut(s) 1016
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 61, 794
BanI GGYRCC 3 cut(s) 67, 162, 1000
BanII GRGCYC 1 cut(s) 920
BauI CACGAG 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 945
Bbv12I GWGCWC 1 cut(s) 920
BbvI GCAGC 6 cut(s) 468, 496, 618, 823, 964, 993
BccI CCATC 5 cut(s) 270, 432, 951, 1069, 1147
BceAI ACGGC 3 cut(s) 55, 427, 1060
BciT130I CCWGG 1 cut(s) 429
BcnI CCSGG 3 cut(s) 65, 453, 1034
BcoDI GTCTC 2 cut(s) 130, 374
BfaI CTAG 1 cut(s) 821
BfoI RGCGCY 2 cut(s) 71, 166
BglI GCCNNNNNGGC 1 cut(s) 1088
BisI GCNGC 7 cut(s) 457, 485, 632, 837, 953, 1004, 1007
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 7 cut(s) 458, 486, 633, 838, 954, 1005, 1008
Bme1390I CCNGG 4 cut(s) 65, 429, 453, 1034
Bme18I GGWCC 2 cut(s) 61, 794
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 909
BmgT120I GGNCC 2 cut(s) 61, 794
BmiI GGNNCC 5 cut(s) 63, 69, 164, 732, 1002
BmrFI CCNGG 4 cut(s) 65, 429, 453, 1034
BmsI GCATC 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 945
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
Bpu1102I GCTNAGC 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 1016
BpuEI CTTGAG 1 cut(s) 205
BpuMI CCSGG 3 cut(s) 65, 453, 1034
BsaHI GRCGYC 2 cut(s) 68, 163
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 597, 1060
BsaWI WCCGGW 2 cut(s) 268, 336
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 452, 533, 962
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 2 cut(s) 497, 888
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 2 cut(s) 597, 1060
BseGI GGATG 3 cut(s) 424, 1117, 1139
BseLI CCNNNNNNNGG 4 cut(s) 58, 452, 533, 962
BseMII CTCAG 3 cut(s) 365, 385, 651
BseNI ACTGG 2 cut(s) 497, 888
BseRI GAGGAG 6 cut(s) 179, 504, 652, 831, 910, 1052
BseXI GCAGC 6 cut(s) 468, 496, 618, 823, 964, 993
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 414, 1082
BshNI GGYRCC 3 cut(s) 67, 162, 1000
BsiHKAI GWGCWC 1 cut(s) 920
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 7 cut(s) 65, 269, 337, 453, 725, 833, 1034
BslFI GGGAC 2 cut(s) 47, 435
BslI CCNNNNNNNGG 4 cut(s) 58, 452, 533, 962
BsmAI GTCTC 2 cut(s) 130, 374
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 2 cut(s) 47, 435
BsnI GGCC 2 cut(s) 414, 1082
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 1016
Bsp1286I GDGCHC 1 cut(s) 920
Bsp143I GATC 1 cut(s) 330
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 1060
BspACI CCGC 8 cut(s) 108, 181, 232, 605, 614, 634, 963, 1004
BspANI GGCC 2 cut(s) 414, 1082
BspCNI CTCAG 3 cut(s) 364, 384, 652
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 2 cut(s) 573, 876
BspLI GGNNCC 5 cut(s) 63, 69, 164, 732, 1002
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 908
BspT104I TTCGAA 1 cut(s) 1016
BspT107I GGYRCC 3 cut(s) 67, 162, 1000
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 963
BsrFI RCCGGY 1 cut(s) 724
BsrI ACTGG 2 cut(s) 497, 888
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 2 cut(s) 597, 1060
BssMI GATC 1 cut(s) 330
BssNI GRCGYC 2 cut(s) 68, 163
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 1060
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 2 cut(s) 889, 908
BstACI GRCGYC 2 cut(s) 68, 163
BstBI TTCGAA 1 cut(s) 1016
BstC8I GCNNGC 4 cut(s) 181, 236, 666, 1084
BstDEI CTNAG 3 cut(s) 351, 371, 660
BstDSI CCRYGG 2 cut(s) 597, 1060
BstF5I GGATG 3 cut(s) 424, 1117, 1139
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 2 cut(s) 71, 166
BstHHI GCGC 2 cut(s) 70, 165
BstKTI GATC 1 cut(s) 333
BstMAI GTCTC 2 cut(s) 130, 374
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 4 cut(s) 231, 411, 611, 1088
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 4 cut(s) 63, 427, 451, 1032
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 6 cut(s) 468, 496, 618, 823, 964, 993
BstV2I GAAGAC 1 cut(s) 945
BstX2I RGATCY 1 cut(s) 330
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 2 cut(s) 414, 1082
BtgI CCRYGG 2 cut(s) 597, 1060
BtrI CACGTC 1 cut(s) 909
BtsCI GGATG 3 cut(s) 424, 1117, 1139
Cac8I GCNNGC 4 cut(s) 181, 236, 666, 1084
CciI TCATGA 2 cut(s) 573, 876
CfoI GCGC 2 cut(s) 70, 165
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 2 cut(s) 61, 794
Csp6I GTAC 3 cut(s) 266, 826, 1042
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 9 cut(s) 406, 574, 649, 716, 757, 877, 1061, 1072, 1132
CviQI GTAC 3 cut(s) 266, 826, 1042
DdeI CTNAG 3 cut(s) 351, 371, 660
DinI GGCGCC 2 cut(s) 69, 164
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 889, 908
EarI CTCTTC 2 cut(s) 889, 908
EciI GGCGGA 2 cut(s) 221, 629
Ecl136II GAGCTC 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 920
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 2 cut(s) 61, 794
Eco53kI GAGCTC 1 cut(s) 918
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 920
EgeI GGCGCC 2 cut(s) 69, 164
EheI GGCGCC 2 cut(s) 69, 164
ErhI CCWWGG 1 cut(s) 1060
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 9 cut(s) 409, 577, 652, 719, 760, 880, 1064, 1075, 1135
FaqI GGGAC 2 cut(s) 47, 435
FatI CATG 9 cut(s) 405, 573, 648, 715, 756, 876, 1060, 1071, 1131
FauI CCCGC 3 cut(s) 115, 188, 598
FauNDI CATATG 1 cut(s) 672
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 7 cut(s) 457, 485, 632, 837, 953, 1004, 1007
FokI GGATG 3 cut(s) 411, 1124, 1126
FriOI GRGCYC 1 cut(s) 920
Fsp4HI GCNGC 7 cut(s) 457, 485, 632, 837, 953, 1004, 1007
FspBI CTAG 1 cut(s) 821
GlaI GCGC 2 cut(s) 69, 164
GluI GCNGC 7 cut(s) 457, 485, 632, 837, 953, 1004, 1007
HaeII RGCGCY 2 cut(s) 71, 166
HaeIII GGCC 2 cut(s) 414, 1082
HapII CCGG 7 cut(s) 65, 269, 337, 453, 725, 833, 1034
HhaI GCGC 2 cut(s) 70, 165
Hin1I GRCGYC 2 cut(s) 68, 163
Hin1II CATG 9 cut(s) 409, 577, 652, 719, 760, 880, 1064, 1075, 1135
Hin6I GCGC 2 cut(s) 68, 163
HinP1I GCGC 2 cut(s) 68, 163
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 5 cut(s) 47, 473, 500, 506, 1122
HpaII CCGG 7 cut(s) 65, 269, 337, 453, 725, 833, 1034
HphI GGTGA 2 cut(s) 938, 1049
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 8 cut(s) 472, 478, 505, 661, 711, 735, 894, 960
Hpy188III TCNNGA 7 cut(s) 44, 76, 170, 574, 877, 935, 1143
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 5 cut(s) 101, 104, 140, 143, 913
HpyAV CCTTC 4 cut(s) 160, 451, 759, 1102
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 1 cut(s) 908
HpyCH4V TGCA 3 cut(s) 768, 978, 1030
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 411, 611, 1088
HpyF3I CTNAG 3 cut(s) 351, 371, 660
HpySE526I ACGT 1 cut(s) 908
Hsp92I GRCGYC 2 cut(s) 68, 163
Hsp92II CATG 9 cut(s) 409, 577, 652, 719, 760, 880, 1064, 1075, 1135
HspAI GCGC 2 cut(s) 68, 163
KasI GGCGCC 2 cut(s) 67, 162
Kzo9I GATC 1 cut(s) 330
LguI GCTCTTC 1 cut(s) 908
LmnI GCTCC 4 cut(s) 639, 736, 844, 923
Lsp1109I GCAGC 6 cut(s) 468, 496, 618, 823, 964, 993
LweI GCATC 1 cut(s) 468
MaeI CTAG 1 cut(s) 821
MaeII ACGT 1 cut(s) 908
MaeIII GTNAC 2 cut(s) 925, 965
MalI GATC 1 cut(s) 332
MbiI CCGCTC 1 cut(s) 963
MboI GATC 1 cut(s) 330
MboII GAAGA 7 cut(s) 271, 289, 744, 876, 925, 950, 1030
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 920
MluCI AATT 2 cut(s) 568, 805
Mly113I GGCGCC 2 cut(s) 68, 163
MlyI GAGTC 4 cut(s) 41, 467, 500, 1116
MmeI TCCRAC 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 3 cut(s) 540, 552, 582
MslI CAYNNNNRTG 2 cut(s) 653, 1144
MspA1I CMGCKG 1 cut(s) 1006
MspI CCGG 7 cut(s) 65, 269, 337, 453, 725, 833, 1034
MspR9I CCNGG 4 cut(s) 65, 429, 453, 1034
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 231, 411, 611, 1088
NarI GGCGCC 2 cut(s) 68, 163
NciI CCSGG 3 cut(s) 65, 453, 1034
NcoI CCATGG 1 cut(s) 1060
NdeI CATATG 1 cut(s) 672
NdeII GATC 1 cut(s) 330
NlaIII CATG 9 cut(s) 409, 577, 652, 719, 760, 880, 1064, 1075, 1135
NlaIV GGNNCC 5 cut(s) 63, 69, 164, 732, 1002
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 1 cut(s) 925
NspV TTCGAA 1 cut(s) 1016
PagI TCATGA 2 cut(s) 573, 876
PciSI GCTCTTC 1 cut(s) 908
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 7 cut(s) 458, 486, 633, 838, 954, 1005, 1008
PleI GAGTC 4 cut(s) 41, 467, 500, 1116
PluTI GGCGCC 2 cut(s) 71, 166
PpsI GAGTC 4 cut(s) 41, 467, 500, 1116
Psp124BI GAGCTC 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 5 cut(s) 63, 69, 164, 732, 1002
PspPI GGNCC 2 cut(s) 61, 794
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 3 cut(s) 267, 827, 1043
RsaNI GTAC 3 cut(s) 266, 826, 1042
RseI CAYNNNNRTG 2 cut(s) 653, 1144
SacI GAGCTC 1 cut(s) 920
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 908
SaqAI TTAA 3 cut(s) 540, 552, 582
SatI GCNGC 7 cut(s) 457, 485, 632, 837, 953, 1004, 1007
Sau3AI GATC 1 cut(s) 330
Sau96I GGNCC 2 cut(s) 61, 794
SchI GAGTC 4 cut(s) 41, 467, 500, 1116
ScrFI CCNGG 4 cut(s) 65, 429, 453, 1034
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 1 cut(s) 468
SfoI GGCGCC 2 cut(s) 69, 164
SfuI TTCGAA 1 cut(s) 1016
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 2 cut(s) 61, 794
SmiMI CAYNNNNRTG 2 cut(s) 653, 1144
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 2 cut(s) 568, 805
SsiI CCGC 8 cut(s) 108, 181, 232, 605, 614, 634, 963, 1004
SspDI GGCGCC 2 cut(s) 67, 162
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 821
SstI GAGCTC 1 cut(s) 920
StyD4I CCNGG 4 cut(s) 63, 427, 451, 1032
StyI CCWWGG 1 cut(s) 1060
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 1 cut(s) 911
TaqI TCGA 5 cut(s) 99, 911, 936, 1016, 1049
TaqII GACCGA 1 cut(s) 49
TasI AATT 2 cut(s) 568, 805
TauI GCSGC 1 cut(s) 1006
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 3 cut(s) 540, 552, 582
Tru9I TTAA 3 cut(s) 540, 552, 582
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 6 cut(s) 456, 484, 631, 836, 952, 1006
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 5 cut(s) 422, 562, 590, 893, 1001
TspGWI ACGGA 1 cut(s) 913
VpaK11BI GGWCC 2 cut(s) 61, 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.