FvH4_2g34470

CYPRO4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014939
Physical Location & Seq
Forward (+)
25596482 .. 25599997
3516 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34470.t1

Sequence Viewer

Length: 1914 bp
ATGGGTGGGGCTCACAGCCGCGAAGACCTCGAGCTCATTGACTCAGACGACGAAGAGCAACAGAGCGAAGGGGAGGAGTGTTTCCAGGACGCGGAGGAGAAGATTCAGAGATCGGCGGAGCGCGGTGGGCCCAAGACGCCGGCGTCGGTGGATGACGTGGAGGCGAAGCTGAAGGCGTTGAAGCTGAAATACGCGTCGGCGTCGACCCCGAATCTGAAGAATGCGGTGAAGCTGTACGTTCATGTTGGTGGGAACACGCCCAAAGCGAAATGGGTAACCTCTGAGAAACTCACATCTTATTGTTTTCGAAAGACGTGTAGAATGGAGGTTGATGATGAAGAGGAAGATGTGGAGGATGAGGATGAGGAGAGGGAGGATTCGTGGTGGGTGTTGAGGGTGGGGGAGAAGATTAGGGTGAAAGTTTCGGCTGAAATGCAGTTGAAGACTTTTGGGAGGGAGCGGCGTGTCGATTTTGTGGCCCGGGGAGTGTGGGCTATGAAGTTCTTTAATGAGGAGGACTATAGGGTTTTTGTTGCTAAGTATACTGATTGTATGTTTGAGAATACTTATGGATATGAGGCGACCGAGCAGAACAGGGTTAAGGTGTATGGGAAGGATTTTATTGGGTGGGCAAAGCCGGAGGCGGCGGATGATTCTGTTTGGGAAGATGCTGAGGATGGTTTGCTGAAGAGCCCGGGGTCTGCCACCCCGGTCAGAGTGAATCAGGACTTGAGGGAGGAGTTTGAGGAGGCGGCACATGGAGGGATACAGAGCTTGGCGTTGGGTGCATTGGAGAACAGTTTCTTGGTTGGGGACTCTGGCGTTCAGGTTGTTAAGAATTACAGTTATGGAATACAGGGGAAAGGTGTTTATGTCAGTTTTGATGATGGGAGTTATAGAGGTGGGTCGAGTGTGGCCCGTTCCACGCCTAGGAAGGCTCTGCTGATGAAGGCGGAAACTAATATGCTCCTCATGAGTCCAATGAAAGAGGGGAAGCCTCACACAACAGGGCTTCAACAGTTTGACATCGAAGCTGGAAAGGTTGTGACTGAGTGGAAGTTTGAAAAGGATGGGACTGATATTACAATGAAGGATATCGCAAATGACAGCAAGGGAGCTCAGTTGGATCCTTCAGGGTCCACATTCTTGGGATTGGATGATAACAGGCTTTGCCGTTGGGATATGCGAGACAGGAAAGGGATGGTTCAGGATCTTGCAAGTGCTAATACTCCTATTCTCAATTGGAATCAAGGGCATCAGTTCTCCAGAGGAACTAACTTTCAGTGCTTTGCTAGTACTGGTGATGGCTCAATTGTTGTGGGGTCTCTTGATGGGAAGATCAGGTTGTATTCAATCAATTCTATGAGGCAAGCCAAAACAGCTTTTCCTGGCCTTGGTTCTCCTATTACGCATGTGGATGTTACATATGATGGAAAGTGGATTTTGGGAACAACTGACACCTATTTGATCCTCATCTGTACCATGTTTACTGATAAGGATGGGAAGACAAAGACTGGCTTTGCTGGGCGTATGGGGAATAGGATTTCTGCCCCAAGGTTGTTGAAGCTCACTCCTCTGGATTCTCACTTGGCAGGCACTAATAACAAGTTTCGTAATGCTCAGTTTTCATGGGTCACAGAGAATGGGAAGCAGGAGCGCCATTTAGTTGCAACTGTCGGAAAGTTTAGTGTGATTTGGAATTTCCAACAGGTGAAGGATGGGTCACATGAGTGCTACCTAAATCAGGAGGGCTTGAAGAGCTGTTATTGTTATAAGATAGTCCTGAAGGATGATTCCATTGTTGATAGTCGCTTCATGCACGACAAGTTTGCAGTTACCGACTCACCTGAAGCCCCACTTGTGATTGCAACCCCATTGAAGGTCAGCTCCTTCAGCATATCCAGTAGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

638

Amino Acids

71.2

Weight (kDa)

5.44

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 43 - 204 2.6e-76 Domain of unknown function (DUF7135)
VID27 PF08553 309 - 580 5.2e-42 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33400
fragaria_vesca FvH4_2g34470
malus_domestica MD08G1101800.v1.1 MD15G1083300.v1.1
prunus_persica Prupe.1G435800_v2.0.a1
pyrus_communis pycom08g08230 pycom08g08240 pycom15g07780
rosa_chinensis RchiOBHm_Chr6g0307901
rosa_laevigata RLG00000010688
rosa_rugosa Rorug06G0361500
rosa_samantha Rh6AG470600 Rh6BG481600 Rh6CG487000 Rh6DG473100
rosa_wichuraiana Rw6G041090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1773
AasI GACNNNNNNGTC 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 2 cut(s) 203, 542
AccII CGCG 4 cut(s) 21, 92, 123, 194
AclWI GGATC 4 cut(s) 1121, 1134, 1218, 1462
AcsI RAATTY 1 cut(s) 1699
AcuI CTGAAG 7 cut(s) 191, 236, 707, 1116, 1805, 1869, 1876
AcyI GRCGYC 3 cut(s) 137, 143, 200
AfaI GTAC 3 cut(s) 236, 1297, 1480
AfiI CCNNNNNNNGG 5 cut(s) 91, 930, 1394, 1744, 1879
AflIII ACRYGT 2 cut(s) 192, 314
AgsI TTSAA 8 cut(s) 181, 442, 1016, 1064, 1353, 1564, 1756, 1879
AjiI CACGTC 2 cut(s) 157, 315
AjnI CCWGG 2 cut(s) 84, 1387
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AleI CACNNNNGTG 1 cut(s) 1729
Alw21I GWGCWC 2 cut(s) 36, 1120
Alw26I GTCTC 2 cut(s) 1182, 1329
AlwI GGATC 4 cut(s) 1121, 1134, 1218, 1462
Ama87I CYCGRG 3 cut(s) 29, 480, 694
AoxI GGCC 4 cut(s) 128, 477, 915, 1390
ApaI GGGCCC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 1699
Asp700I GAANNNNTTC 1 cut(s) 800
AspA2I CCTAGG 1 cut(s) 929
AspLEI GCGC 2 cut(s) 123, 1659
AspS9I GGNCC 5 cut(s) 128, 129, 478, 916, 1137
AsuC2I CCSGG 5 cut(s) 481, 482, 695, 696, 710
AsuHPI GGTGA 5 cut(s) 238, 427, 1313, 1723, 1836
AsuII TTCGAA 1 cut(s) 307
AvaI CYCGRG 3 cut(s) 29, 480, 694
AvaII GGWCC 1 cut(s) 1137
AvrII CCTAGG 1 cut(s) 929
BaeGI GKGCMC 1 cut(s) 132
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 5 cut(s) 13, 36, 132, 695, 1120
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbsI GAAGAC 3 cut(s) 30, 449, 1511
Bbv12I GWGCWC 2 cut(s) 36, 1120
BbvCI CCTCAGC 1 cut(s) 672
BccI CCATC 9 cut(s) 671, 881, 1064, 1195, 1298, 1325, 1424, 1493, 1712
BceAI ACGGC 1 cut(s) 1158
BcgI CGANNNNNNTGC 2 cut(s) 1811, 1845
BciT130I CCWGG 2 cut(s) 86, 1389
BciVI GTATCC 1 cut(s) 759
BcnI CCSGG 5 cut(s) 481, 482, 695, 696, 710
BcoDI GTCTC 2 cut(s) 1182, 1329
BfaI CTAG 2 cut(s) 930, 1293
BfmI CTRYAG 1 cut(s) 520
BfoI RGCGCY 1 cut(s) 1660
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 4 cut(s) 19, 461, 645, 753
BlnI CCTAGG 1 cut(s) 929
BlsI GCNGC 4 cut(s) 20, 462, 646, 754
BmcAI AGTACT 1 cut(s) 1297
Bme1390I CCNGG 7 cut(s) 86, 481, 482, 695, 696, 710, 1389
Bme18I GGWCC 1 cut(s) 1137
BmeT110I CYCGRG 3 cut(s) 29, 480, 694
BmgBI CACGTC 2 cut(s) 157, 315
BmgT120I GGNCC 5 cut(s) 128, 129, 478, 916, 1137
BmiI GGNNCC 3 cut(s) 130, 1128, 1138
BmrFI CCNGG 7 cut(s) 86, 481, 482, 695, 696, 710, 1389
BmsI GCATC 2 cut(s) 658, 1264
BpiI GAAGAC 3 cut(s) 30, 449, 1511
BpmI CTGGAG 1 cut(s) 1249
Bpu10I CCTNAGC 1 cut(s) 672
Bpu14I TTCGAA 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 5 cut(s) 481, 482, 695, 696, 710
BsaBI GATNNNNATC 1 cut(s) 1472
BsaHI GRCGYC 3 cut(s) 137, 143, 200
BsaI GGTCTC 1 cut(s) 1329
BsaJI CCNNGG 8 cut(s) 480, 481, 694, 695, 708, 929, 1393, 1553
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 930, 1394, 1744, 1879
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 3 cut(s) 1303, 1519, 1902
Bse8I GATNNNNATC 1 cut(s) 1472
BseBI CCWGG 2 cut(s) 86, 1389
BseDI CCNNGG 8 cut(s) 480, 481, 694, 695, 708, 929, 1393, 1553
BseJI GATNNNNATC 1 cut(s) 1472
BseLI CCNNNNNNNGG 5 cut(s) 91, 930, 1394, 1744, 1879
BseMII CTCAG 6 cut(s) 57, 273, 663, 1041, 1133, 1634
BseNI ACTGG 3 cut(s) 1303, 1519, 1902
BseRI GAGGAG 8 cut(s) 89, 110, 380, 527, 752, 761, 959, 1563
BseSI GKGCMC 1 cut(s) 132
BseYI CCCAGC 1 cut(s) 1523
Bsh1236I CGCG 4 cut(s) 21, 92, 123, 194
Bsh1285I CGRYCG 1 cut(s) 585
BshFI GGCC 4 cut(s) 130, 479, 917, 1392
BsiEI CGRYCG 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 36, 1120
BsiHKCI CYCGRG 3 cut(s) 29, 480, 694
BsiSI CCGG 5 cut(s) 140, 481, 638, 695, 710
BslFI GGGAC 2 cut(s) 827, 1087
BslI CCNNNNNNNGG 5 cut(s) 91, 930, 1394, 1744, 1879
BsmAI GTCTC 2 cut(s) 1182, 1329
BsmFI GGGAC 2 cut(s) 827, 1087
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 4 cut(s) 130, 479, 917, 1392
Bso31I GGTCTC 1 cut(s) 1329
BsoBI CYCGRG 3 cut(s) 29, 480, 694
Bsp119I TTCGAA 1 cut(s) 307
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 5 cut(s) 13, 36, 132, 695, 1120
Bsp143I GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
BspANI GGCC 4 cut(s) 130, 479, 917, 1392
BspCNI CTCAG 6 cut(s) 56, 274, 664, 1042, 1132, 1633
BspFNI CGCG 4 cut(s) 21, 92, 123, 194
BspHI TCATGA 1 cut(s) 972
BspLI GGNNCC 3 cut(s) 130, 1128, 1138
BspPI GGATC 4 cut(s) 1121, 1134, 1218, 1462
BspQI GCTCTTC 3 cut(s) 48, 683, 1751
BspT104I TTCGAA 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 1329
BsrBI CCGCTC 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 3 cut(s) 1303, 1519, 1902
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 8 cut(s) 480, 481, 694, 695, 708, 929, 1393, 1553
BssMI GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
BssNAI GTATAC 1 cut(s) 543
BssNI GRCGYC 3 cut(s) 137, 143, 200
BssT1I CCWWGG 3 cut(s) 929, 1393, 1553
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 86, 1389
Bst4CI ACNGT 4 cut(s) 800, 845, 1020, 1675
Bst6I CTCTTC 4 cut(s) 48, 333, 683, 1751
BstACI GRCGYC 3 cut(s) 137, 143, 200
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 141, 1371, 1594
BstDEI CTNAG 7 cut(s) 43, 282, 537, 672, 1050, 1119, 1620
BstEII GGTNACC 1 cut(s) 274
BstENI CCTNNNNNAGG 1 cut(s) 1742
BstFNI CGCG 4 cut(s) 21, 92, 123, 194
BstH2I RGCGCY 1 cut(s) 1660
BstHHI GCGC 2 cut(s) 123, 1659
BstKTI GATC 5 cut(s) 113, 1129, 1213, 1341, 1470
BstMAI GTCTC 2 cut(s) 1182, 1329
BstMBI GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 6 cut(s) 127, 136, 785, 1379, 1758, 1893
BstNI CCWGG 2 cut(s) 86, 1389
BstNSI RCATGY 1 cut(s) 1415
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 7 cut(s) 84, 479, 480, 693, 694, 708, 1387
BstSFI CTRYAG 1 cut(s) 520
BstSLI GKGCMC 1 cut(s) 132
BstUI CGCG 4 cut(s) 21, 92, 123, 194
BstV2I GAAGAC 3 cut(s) 30, 449, 1511
BstX2I RGATCY 2 cut(s) 1126, 1210
BstXI CCANNNNNNTGG 1 cut(s) 1147
BstYI RGATCY 2 cut(s) 1126, 1210
BstZ17I GTATAC 1 cut(s) 543
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 4 cut(s) 130, 479, 917, 1392
BtrI CACGTC 2 cut(s) 157, 315
BtsIMutI CAGTG 1 cut(s) 1289
Cac8I GCNNGC 3 cut(s) 141, 1371, 1594
CciI TCATGA 1 cut(s) 972
CfoI GCGC 2 cut(s) 123, 1659
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 5 cut(s) 128, 129, 478, 916, 1137
Cfr9I CCCGGG 2 cut(s) 480, 694
CseI GACGC 5 cut(s) 98, 132, 145, 183, 189
Csp6I GTAC 3 cut(s) 235, 1296, 1479
CspCI CAANNNNNGTGG 2 cut(s) 1299, 1334
CviAII CATG 8 cut(s) 242, 758, 973, 1412, 1483, 1629, 1727, 1816
CviQI GTAC 3 cut(s) 235, 1296, 1479
DdeI CTNAG 7 cut(s) 43, 282, 537, 672, 1050, 1119, 1620
DpnI GATC 5 cut(s) 112, 1128, 1212, 1340, 1469
DpnII GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 4 cut(s) 48, 333, 683, 1751
EarI CTCTTC 4 cut(s) 48, 333, 683, 1751
EciI GGCGGA 3 cut(s) 131, 662, 968
Ecl136II GAGCTC 2 cut(s) 34, 1118
Eco130I CCWWGG 3 cut(s) 929, 1393, 1553
Eco24I GRGCYC 5 cut(s) 13, 36, 132, 695, 1120
Eco31I GGTCTC 1 cut(s) 1329
Eco32I GATATC 1 cut(s) 1096
Eco47I GGWCC 1 cut(s) 1137
Eco53kI GAGCTC 2 cut(s) 34, 1118
Eco57I CTGAAG 7 cut(s) 191, 236, 707, 1116, 1805, 1869, 1876
Eco88I CYCGRG 3 cut(s) 29, 480, 694
Eco91I GGTNACC 1 cut(s) 274
EcoICRI GAGCTC 2 cut(s) 34, 1118
EcoNI CCTNNNNNAGG 1 cut(s) 1742
EcoO65I GGTNACC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 84, 1387
EcoRV GATATC 1 cut(s) 1096
EcoT14I CCWWGG 3 cut(s) 929, 1393, 1553
EcoT38I GRGCYC 5 cut(s) 13, 36, 132, 695, 1120
ErhI CCWWGG 3 cut(s) 929, 1393, 1553
FaeI CATG 8 cut(s) 245, 761, 976, 1415, 1486, 1632, 1730, 1819
FalI AAGNNNNNCTT 4 cut(s) 1502, 1534, 1842, 1874
FaqI GGGAC 2 cut(s) 827, 1087
FatI CATG 8 cut(s) 241, 757, 972, 1411, 1482, 1628, 1726, 1815
FauNDI CATATG 1 cut(s) 1426
FblI GTMKAC 2 cut(s) 203, 542
Fnu4HI GCNGC 4 cut(s) 19, 461, 645, 753
FriOI GRGCYC 5 cut(s) 13, 36, 132, 695, 1120
Fsp4HI GCNGC 4 cut(s) 19, 461, 645, 753
FspBI CTAG 2 cut(s) 930, 1293
GlaI GCGC 2 cut(s) 122, 1658
GluI GCNGC 4 cut(s) 19, 461, 645, 753
GsaI CCCAGC 1 cut(s) 1527
GsuI CTGGAG 1 cut(s) 1249
HaeII RGCGCY 1 cut(s) 1660
HaeIII GGCC 4 cut(s) 130, 479, 917, 1392
HapII CCGG 5 cut(s) 140, 481, 638, 695, 710
HgaI GACGC 5 cut(s) 98, 132, 145, 183, 189
HhaI GCGC 2 cut(s) 123, 1659
Hin1I GRCGYC 3 cut(s) 137, 143, 200
Hin1II CATG 8 cut(s) 245, 761, 976, 1415, 1486, 1632, 1730, 1819
Hin6I GCGC 2 cut(s) 121, 1657
HinP1I GCGC 2 cut(s) 121, 1657
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HpaII CCGG 5 cut(s) 140, 481, 638, 695, 710
HphI GGTGA 5 cut(s) 238, 427, 1313, 1723, 1836
Hpy166II GTNNAC 4 cut(s) 204, 543, 1140, 1488
Hpy188I TCNGA 6 cut(s) 46, 108, 216, 283, 716, 1679
Hpy188III TCNNGA 8 cut(s) 725, 973, 1208, 1266, 1328, 1577, 1745, 1783
Hpy8I GTNNAC 4 cut(s) 204, 543, 1140, 1488
Hpy99I CGWCG 4 cut(s) 53, 148, 199, 205
HpyCH4III ACNGT 4 cut(s) 800, 845, 1020, 1675
HpyCH4IV ACGT 3 cut(s) 156, 237, 314
HpyCH4V TGCA 7 cut(s) 436, 788, 1217, 1670, 1819, 1832, 1868
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 136, 785, 1379, 1758, 1893
HpyF3I CTNAG 7 cut(s) 43, 282, 537, 672, 1050, 1119, 1620
HpySE526I ACGT 3 cut(s) 156, 237, 314
Hsp92I GRCGYC 3 cut(s) 137, 143, 200
Hsp92II CATG 8 cut(s) 245, 761, 976, 1415, 1486, 1632, 1730, 1819
HspAI GCGC 2 cut(s) 121, 1657
KroI GCCGGC 1 cut(s) 139
KroNI GCCGGC 1 cut(s) 141
Kzo9I GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
LguI GCTCTTC 3 cut(s) 48, 683, 1751
LmnI GCTCC 6 cut(s) 118, 457, 972, 1115, 1654, 1892
LweI GCATC 2 cut(s) 658, 1264
MaeI CTAG 2 cut(s) 930, 1293
MaeII ACGT 3 cut(s) 156, 237, 314
MaeIII GTNAC 6 cut(s) 274, 1045, 1420, 1633, 1722, 1834
MalI GATC 5 cut(s) 112, 1128, 1212, 1340, 1469
MbiI CCGCTC 1 cut(s) 460
MboI GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
MfeI CAATTG 2 cut(s) 1240, 1311
MflI RGATCY 2 cut(s) 1126, 1210
MhlI GDGCHC 5 cut(s) 13, 36, 132, 695, 1120
MluCI AATT 5 cut(s) 838, 1240, 1311, 1357, 1699
MluI ACGCGT 1 cut(s) 192
MlyI GAGTC 4 cut(s) 35, 809, 985, 1835
MmeI TCCRAC 3 cut(s) 1104, 1657, 1729
MreI CGCCGGCG 1 cut(s) 139
MroNI GCCGGC 1 cut(s) 139
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 3 cut(s) 507, 600, 834
MslI CAYNNNNRTG 3 cut(s) 246, 1416, 1729
MspI CCGG 5 cut(s) 140, 481, 638, 695, 710
MspR9I CCNGG 7 cut(s) 86, 481, 482, 695, 696, 710, 1389
MunI CAATTG 2 cut(s) 1240, 1311
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 2 cut(s) 86, 1389
MvnI CGCG 4 cut(s) 21, 92, 123, 194
MwoI GCNNNNNNNGC 6 cut(s) 127, 136, 785, 1379, 1758, 1893
NaeI GCCGGC 1 cut(s) 141
NciI CCSGG 5 cut(s) 481, 482, 695, 696, 710
NdeI CATATG 1 cut(s) 1426
NdeII GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
NgoMIV GCCGGC 1 cut(s) 139
NlaIII CATG 8 cut(s) 245, 761, 976, 1415, 1486, 1632, 1730, 1819
NlaIV GGNNCC 3 cut(s) 130, 1128, 1138
NmuCI GTSAC 3 cut(s) 1045, 1633, 1722
NspI RCATGY 1 cut(s) 1415
NspV TTCGAA 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 1729
PaeR7I CTCGAG 1 cut(s) 29
PagI TCATGA 1 cut(s) 972
PciSI GCTCTTC 3 cut(s) 48, 683, 1751
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PctI GAATGC 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 8 cut(s) 103, 211, 377, 653, 721, 1246, 1580, 1793
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 4 cut(s) 20, 462, 646, 754
PleI GAGTC 4 cut(s) 35, 809, 984, 1835
PpsI GAGTC 4 cut(s) 35, 809, 984, 1835
PsiI TTATAA 1 cut(s) 1773
Psp124BI GAGCTC 2 cut(s) 36, 1120
Psp6I CCWGG 2 cut(s) 84, 1387
PspEI GGTNACC 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 1523
PspGI CCWGG 2 cut(s) 84, 1387
PspN4I GGNNCC 3 cut(s) 130, 1128, 1138
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 5 cut(s) 128, 129, 478, 916, 1137
PspXI VCTCGAGB 1 cut(s) 29
PsuI RGATCY 2 cut(s) 1126, 1210
RsaI GTAC 3 cut(s) 236, 1297, 1480
RsaNI GTAC 3 cut(s) 235, 1296, 1479
RseI CAYNNNNRTG 3 cut(s) 246, 1416, 1729
SacI GAGCTC 2 cut(s) 36, 1120
SalI GTCGAC 1 cut(s) 202
SapI GCTCTTC 3 cut(s) 48, 683, 1751
SaqAI TTAA 3 cut(s) 507, 600, 834
SatI GCNGC 4 cut(s) 19, 461, 645, 753
Sau3AI GATC 5 cut(s) 110, 1126, 1210, 1338, 1467
Sau96I GGNCC 5 cut(s) 128, 129, 478, 916, 1137
ScaI AGTACT 1 cut(s) 1297
SchI GAGTC 4 cut(s) 35, 809, 985, 1835
ScrFI CCNGG 7 cut(s) 86, 481, 482, 695, 696, 710, 1389
SduI GDGCHC 5 cut(s) 13, 36, 132, 695, 1120
SfaNI GCATC 2 cut(s) 658, 1264
SfcI CTRYAG 1 cut(s) 520
Sfr274I CTCGAG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 307
SgrAI CRCCGGYG 1 cut(s) 139
SinI GGWCC 1 cut(s) 1137
SlaI CTCGAG 1 cut(s) 29
SmaI CCCGGG 2 cut(s) 482, 696
SmiMI CAYNNNNRTG 3 cut(s) 246, 1416, 1729
SmlI CTYRAG 2 cut(s) 29, 730
SmoI CTYRAG 2 cut(s) 29, 730
Sse9I AATT 5 cut(s) 838, 1240, 1311, 1357, 1699
SspMI CTAG 2 cut(s) 930, 1293
SstI GAGCTC 2 cut(s) 36, 1120
StyD4I CCNGG 7 cut(s) 84, 479, 480, 693, 694, 708, 1387
StyI CCWWGG 3 cut(s) 929, 1393, 1553
TaaI ACNGT 4 cut(s) 800, 845, 1020, 1675
TaiI ACGT 3 cut(s) 159, 240, 317
TaqI TCGA 6 cut(s) 30, 203, 307, 468, 908, 1029
TaqII GACCGA 1 cut(s) 599
TasI AATT 5 cut(s) 838, 1240, 1311, 1357, 1699
TatI WGTACW 1 cut(s) 1295
TauI GCSGC 4 cut(s) 21, 463, 647, 755
TfiI GAWTC 8 cut(s) 103, 211, 377, 653, 721, 1246, 1580, 1793
Tru1I TTAA 3 cut(s) 507, 600, 834
Tru9I TTAA 3 cut(s) 507, 600, 834
TscAI CASTG 1 cut(s) 1289
TseFI GTSAC 3 cut(s) 1045, 1633, 1722
Tsp45I GTSAC 3 cut(s) 1045, 1633, 1722
TspDTI ATGAA 8 cut(s) 230, 351, 512, 962, 998, 1103, 1617, 1804
TspMI CCCGGG 2 cut(s) 480, 694
TspRI CASTG 1 cut(s) 1289
VpaK11BI GGWCC 1 cut(s) 1137
XagI CCTNNNNNAGG 1 cut(s) 1742
XapI RAATTY 1 cut(s) 1699
XceI RCATGY 1 cut(s) 1415
XhoI CTCGAG 1 cut(s) 29
XmaI CCCGGG 2 cut(s) 480, 694
XmaJI CCTAGG 1 cut(s) 929
XmiI GTMKAC 2 cut(s) 203, 542
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 2 cut(s) 930, 1293
ZrmI AGTACT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.