MD15G1083300.v1.1

CYPRO4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014939
Physical Location & Seq
Forward (+)
5701983 .. 5704933
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1083300.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGGGCGGAGCTCACAGCCGCGAACACCTCGAGCTTTCCGACTCCGACGAGGAAGAGAGCGAGGAGCAGGAGAGCTACGAGGACGTGGAGGAAACTGAAGCCGACCAGCGCCAGAGATCCGCCGAGCGGGGAACCAAAACGCCGTCGTCGGCTGCATTAGACGACGTGGACGCCAAGCTCAAGGCTTTGAGTCTCAAGTACGGGTCTTCTTCCAGGAACCCGAATCTGAAGAATCCGGTGAAGCTGTACCTCCACATTGGAGGGAACACCCCAAAAGCGAAATGGGTAACTTCCGAAAAACTCACTTCTTTCAATTTTATTAAGACATCTCGAATGGATGTTGATAATGATGAGGAGGAAGAAGAAGAAGAGGAATTTGATGAGGAGGGTTTGTGGGTTTTGAGGGTGGGCGCCAAAGTTAGGGCTAAGGTATCAGCCGAGATGCAATTGCTCACTTTCGCCGATCAGCGGCGTGTCGATTTCTTTGCCAGAGGTGTGTGGGCAGTGAAGTTTTTCAGTGATGAGGAGTATAGGGCTTTTGTGATTAAGTATAATGACTGTTTGTTTGAGAACACTTATGGGTATGAGGCCACTGAGGAGAACAGGGTGAAGGTTTATGGGAAAGATTTTGTCGGGTGGGCAAAGCCCGAAATGGCGGATGATTCGGTCTGGGAAGATGCGGAGGATAATTTTTTGAAGAGCCCTGGCTCAGCAACACCGGTGAGGGGAAATCAAAATTTGAGAGAGGAGTTTGAGGAGGCACACGATGGCGGTATACAGAGCTTGGCATTAGGTGCATTGGACAATAGTTTTTTGGTGGGGAACTCTGGCATTCAGGTTGTTAAGAATTTTGCTCATGGAATACATGGTAAAGGTGTTTATGTGAATTTTGATGATGGGAGCTATGGAGGCGGTTCAAATTTGGCACGGTCTACACCTAGGAAGGCTCTGCTGATGAAGGCCGAGACTAACATGCTCCTTATGAGTCCAATGAATGAGGGGAAGCCTCACACAACAGGGCTCCATCAGCTTGATATCGAAACTGGGAAGGTTGTTACTGAGTGGAAGTTTGAGAAAGATGGGGCTGACATTACAATGAGGGATATCACAAATGATAGTAAGGGAGCACAGTTGGACCCCTCAGGATCGACATTCTTGGGTTTGGATGACAACAGGCTTTGCCGGTGGGATATGCGTGATAGGAAGGGAATGGTTCAGGACCTAGCAGCTTCTAGTGGTCCTGTTCTCAATTGGAACCAAGGGCATCAGTTCTCAAGAGGGACTAACTTTCAGTGCTTTGCTAGCACTGGTGATGGCTCAATTGCTGTTGGGTCTCTTGATGGGAAGATCAGGTTGTACTCTATCAATTCGATGAGGCAGGCAAAAACTGCTTTTCCAGGCCTTGGTTCGCCTATTACTCATGTGGATGTTACTTTTGATGGGAAGTGGATTTTGGGCACAACGGACACCTATTTGATCCTCATCTGCACAATCTTTACTGATAAGGATGGCAAGACAAAGACTGGTTTTAGTGGGCGTATGGGAAATAGAATTTCAGCTCCAAGGCTGCTGAAGTTGACTCCCCTGGATTCACATTTGGCGGGCACGAATAACAAGTTTCGTAATGCTCAGTTTTCGTGGGTCACGGAGAACGGGAAGCAGGAGCGACATCTGGTTGCAACTGTGGGCAAATTTAGTGTGGTTTGGAACTTTCAACAGGTGAAAGATGGCTCACACGAGTGCTACCGCAATCAGCAGGGCTTGAAGAGCTGCTACTGCTACAAGATAGTCCCCAAGGATGACTCCATTGTTGACAGTCGCTTCATGCATGACAAGTTTGCAGTTACTGACTCACCTGAGGCCCCCCTTGTGATTGCAACCCCTCTGAAAGTCAGCTCCTTCAGCATATCCAGTACCAATAGACAATTGTCTCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

647

Amino Acids

71.98

Weight (kDa)

5.35

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 42 - 207 3.8e-76 Domain of unknown function (DUF7135)
VID27 PF08553 311 - 583 1.1e-43 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33400
fragaria_vesca FvH4_2g34470
malus_domestica MD08G1101800.v1.1 MD15G1083300.v1.1
prunus_persica Prupe.1G435800_v2.0.a1
pyrus_communis pycom08g08230 pycom08g08240 pycom15g07780
rosa_chinensis RchiOBHm_Chr6g0307901
rosa_laevigata RLG00000010688
rosa_rugosa Rorug06G0361500
rosa_samantha Rh6AG470600 Rh6BG481600 Rh6CG487000 Rh6DG473100
rosa_wichuraiana Rw6G041090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 2 cut(s) 775, 932
AccII CGCG 1 cut(s) 21
AclWI GGATC 3 cut(s) 111, 1153, 1471
AcsI RAATTY 7 cut(s) 374, 736, 847, 886, 919, 1551, 1691
AcuI CTGAAG 4 cut(s) 117, 248, 1592, 1885
AcyI GRCGYC 2 cut(s) 171, 411
AfaI GTAC 4 cut(s) 200, 248, 1358, 1915
AfiI CCNNNNNNNGG 5 cut(s) 126, 420, 468, 725, 1403
AgeI ACCGGT 1 cut(s) 718
AgsI TTSAA 5 cut(s) 313, 697, 918, 1715, 1765
AjiI CACGTC 2 cut(s) 85, 166
AjnI CCWGG 4 cut(s) 212, 703, 1396, 1584
AjuI GAANNNNNNNTTGG 2 cut(s) 1436, 1468
AleI CACNNNNGTG 1 cut(s) 1738
Alw21I GWGCWC 2 cut(s) 13, 1129
Alw26I GTCTC 4 cut(s) 197, 959, 1338, 1935
AlwI GGATC 3 cut(s) 111, 1153, 1471
AlwNI CAGNNNCTG 1 cut(s) 1847
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 4 cut(s) 588, 960, 1399, 1860
ApeKI GCWGC 4 cut(s) 152, 1226, 1567, 1770
ApoI RAATTY 7 cut(s) 374, 736, 847, 886, 919, 1551, 1691
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AsiGI ACCGGT 1 cut(s) 718
Asp700I GAANNNNTTC 1 cut(s) 512
AspA2I CCTAGG 1 cut(s) 938
AspLEI GCGC 2 cut(s) 111, 413
AspS9I GGNCC 4 cut(s) 1135, 1219, 1238, 1861
AsuHPI GGTGA 6 cut(s) 250, 619, 733, 1322, 1732, 1845
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 3 cut(s) 1135, 1219, 1238
AvrII CCTAGG 1 cut(s) 938
AxyI CCTNAGG 2 cut(s) 1141, 1857
BaeGI GKGCMC 2 cut(s) 1460, 1607
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 3 cut(s) 13, 704, 1023
BarI GAAGNNNNNNTAC 2 cut(s) 1757, 1789
BauI CACGAG 1 cut(s) 1736
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 13, 1129
BbvI GCAGC 4 cut(s) 139, 1238, 1554, 1757
BccI CCATC 9 cut(s) 761, 890, 1032, 1073, 1307, 1334, 1433, 1502, 1721
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 4 cut(s) 214, 705, 1398, 1586
BcoDI GTCTC 4 cut(s) 197, 959, 1338, 1935
BfaI CTAG 4 cut(s) 939, 1223, 1233, 1302
BfmI CTRYAG 1 cut(s) 1934
BfoI RGCGCY 2 cut(s) 112, 414
BisI GCNGC 6 cut(s) 19, 153, 470, 1227, 1568, 1771
BlnI CCTAGG 1 cut(s) 938
BlpI GCTNAGC 1 cut(s) 709
BlsI GCNGC 6 cut(s) 20, 154, 471, 1228, 1569, 1772
Bme1390I CCNGG 4 cut(s) 214, 705, 1398, 1586
Bme18I GGWCC 3 cut(s) 1135, 1219, 1238
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 2 cut(s) 85, 166
BmgT120I GGNCC 4 cut(s) 1135, 1219, 1238, 1861
BmiI GGNNCC 7 cut(s) 133, 218, 412, 1022, 1137, 1256, 1863
BmrFI CCNGG 4 cut(s) 214, 705, 1398, 1586
BmrI ACTGGG 1 cut(s) 1053
BmsI GCATC 3 cut(s) 432, 667, 1273
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 1 cut(s) 1053
BoxI GACNNNNGTC 1 cut(s) 1927
BpiI GAAGAC 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 709
BpuEI CTTGAG 3 cut(s) 164, 179, 1258
BsaBI GATNNNNATC 1 cut(s) 1481
BsaHI GRCGYC 2 cut(s) 171, 411
BsaI GGTCTC 1 cut(s) 1338
BsaJI CCNNGG 7 cut(s) 703, 938, 1258, 1402, 1562, 1584, 1794
BsaWI WCCGGW 2 cut(s) 235, 718
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 420, 468, 725, 1403
Bse118I RCCGGY 2 cut(s) 718, 1182
Bse1I ACTGG 4 cut(s) 1048, 1312, 1528, 1911
Bse21I CCTNAGG 2 cut(s) 1141, 1857
Bse8I GATNNNNATC 1 cut(s) 1481
BseBI CCWGG 4 cut(s) 214, 705, 1398, 1586
BseDI CCNNGG 7 cut(s) 703, 938, 1258, 1402, 1562, 1584, 1794
BseGI GGATG 6 cut(s) 343, 664, 1171, 1432, 1513, 1804
BseJI GATNNNNATC 1 cut(s) 1481
BseLI CCNNNNNNNGG 5 cut(s) 126, 420, 468, 725, 1403
BseMII CTCAG 6 cut(s) 585, 723, 1050, 1155, 1643, 1848
BseNI ACTGG 4 cut(s) 1048, 1312, 1528, 1911
BseRI GAGGAG 7 cut(s) 77, 368, 398, 539, 611, 761, 770
BseSI GKGCMC 2 cut(s) 1460, 1607
BseXI GCAGC 4 cut(s) 139, 1238, 1554, 1757
BsgI GTGCAG 1 cut(s) 1471
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 590, 962, 1401, 1862
BshNI GGYRCC 1 cut(s) 410
BshTI ACCGGT 1 cut(s) 718
BsiHKAI GWGCWC 2 cut(s) 13, 1129
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 236, 719, 1183
BslFI GGGAC 2 cut(s) 1294, 1775
BslI CCNNNNNNNGG 5 cut(s) 126, 420, 468, 725, 1403
BsmAI GTCTC 4 cut(s) 197, 959, 1338, 1935
BsmFI GGGAC 2 cut(s) 1294, 1775
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 4 cut(s) 590, 962, 1401, 1862
Bso31I GGTCTC 1 cut(s) 1338
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 6 cut(s) 13, 704, 1023, 1129, 1460, 1607
Bsp143I GATC 5 cut(s) 116, 463, 1145, 1347, 1476
Bsp1720I GCTNAGC 1 cut(s) 709
BspANI GGCC 4 cut(s) 590, 962, 1401, 1862
BspCNI CTCAG 6 cut(s) 586, 722, 1051, 1154, 1642, 1849
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 7 cut(s) 133, 218, 412, 1022, 1137, 1256, 1863
BspMAI CTGCAG 1 cut(s) 1938
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 3 cut(s) 111, 1153, 1471
BspQI GCTCTTC 2 cut(s) 692, 1760
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 1338
BsrBI CCGCTC 1 cut(s) 127
BsrFI RCCGGY 2 cut(s) 718, 1182
BsrI ACTGG 4 cut(s) 1048, 1312, 1528, 1911
BssAI RCCGGY 2 cut(s) 718, 1182
BssECI CCNNGG 7 cut(s) 703, 938, 1258, 1402, 1562, 1584, 1794
BssMI GATC 5 cut(s) 116, 463, 1145, 1347, 1476
BssNAI GTATAC 1 cut(s) 776
BssNI GRCGYC 2 cut(s) 171, 411
BssSI CACGAG 1 cut(s) 1736
BssT1I CCWWGG 5 cut(s) 938, 1258, 1402, 1562, 1794
Bst1107I GTATAC 1 cut(s) 776
Bst2BI CACGAG 1 cut(s) 1736
Bst2UI CCWGG 4 cut(s) 214, 705, 1398, 1586
Bst4CI ACNGT 5 cut(s) 560, 930, 1131, 1684, 1817
Bst6I CTCTTC 4 cut(s) 48, 363, 692, 1760
BstACI GRCGYC 2 cut(s) 171, 411
BstAPI GCANNNNNTGC 2 cut(s) 794, 1388
BstC8I GCNNGC 3 cut(s) 1303, 1380, 1603
BstDEI CTNAG 7 cut(s) 426, 594, 709, 1059, 1141, 1629, 1857
BstF5I GGATG 6 cut(s) 343, 664, 1171, 1432, 1513, 1804
BstFNI CGCG 1 cut(s) 21
BstH2I RGCGCY 2 cut(s) 112, 414
BstHHI GCGC 2 cut(s) 111, 413
BstKTI GATC 5 cut(s) 119, 466, 1148, 1350, 1479
BstMAI GTCTC 4 cut(s) 197, 959, 1338, 1935
BstMBI GATC 5 cut(s) 116, 463, 1145, 1347, 1476
BstMWI GCNNNNNNNGC 8 cut(s) 794, 909, 1027, 1302, 1388, 1767, 1776, 1902
BstNI CCWGG 4 cut(s) 214, 705, 1398, 1586
BstNSI RCATGY 1 cut(s) 976
BstPAI GACNNNNGTC 1 cut(s) 1927
BstSCI CCNGG 4 cut(s) 212, 703, 1396, 1584
BstSFI CTRYAG 1 cut(s) 1934
BstSLI GKGCMC 2 cut(s) 1460, 1607
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 4 cut(s) 139, 1238, 1554, 1757
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BstZ17I GTATAC 1 cut(s) 776
Bsu36I CCTNAGG 2 cut(s) 1141, 1857
BsuRI GGCC 4 cut(s) 590, 962, 1401, 1862
BtrI CACGTC 2 cut(s) 85, 166
BtsCI GGATG 6 cut(s) 343, 664, 1171, 1432, 1513, 1804
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 5 cut(s) 510, 523, 591, 1298, 1305
Cac8I GCNNGC 3 cut(s) 1303, 1380, 1603
CaiI CAGNNNCTG 1 cut(s) 1847
CfoI GCGC 2 cut(s) 111, 413
Cfr10I RCCGGY 2 cut(s) 718, 1182
Cfr13I GGNCC 4 cut(s) 1135, 1219, 1238, 1861
CseI GACGC 1 cut(s) 179
Csp6I GTAC 4 cut(s) 199, 247, 1357, 1914
CspAI ACCGGT 1 cut(s) 718
CviAII CATG 6 cut(s) 857, 866, 973, 1421, 1825, 1829
CviQI GTAC 4 cut(s) 199, 247, 1357, 1914
DdeI CTNAG 7 cut(s) 426, 594, 709, 1059, 1141, 1629, 1857
DinI GGCGCC 1 cut(s) 412
DpnI GATC 5 cut(s) 118, 465, 1147, 1349, 1478
DpnII GATC 5 cut(s) 116, 463, 1145, 1347, 1476
Eam1104I CTCTTC 4 cut(s) 48, 363, 692, 1760
EarI CTCTTC 4 cut(s) 48, 363, 692, 1760
EciI GGCGGA 3 cut(s) 21, 109, 671
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 5 cut(s) 938, 1258, 1402, 1562, 1794
Eco147I AGGCCT 1 cut(s) 1401
Eco24I GRGCYC 3 cut(s) 13, 704, 1023
Eco31I GGTCTC 1 cut(s) 1338
Eco32I GATATC 2 cut(s) 1036, 1105
Eco47I GGWCC 3 cut(s) 1135, 1219, 1238
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 4 cut(s) 117, 248, 1592, 1885
Eco81I CCTNAGG 2 cut(s) 1141, 1857
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 2 cut(s) 1219, 1861
EcoRII CCWGG 4 cut(s) 212, 703, 1396, 1584
EcoRV GATATC 2 cut(s) 1036, 1105
EcoT14I CCWWGG 5 cut(s) 938, 1258, 1402, 1562, 1794
EcoT22I ATGCAT 1 cut(s) 1830
EcoT38I GRGCYC 3 cut(s) 13, 704, 1023
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 5 cut(s) 938, 1258, 1402, 1562, 1794
FaeI CATG 6 cut(s) 860, 869, 976, 1424, 1828, 1832
FaqI GGGAC 2 cut(s) 1294, 1775
FatI CATG 6 cut(s) 856, 865, 972, 1420, 1824, 1828
FauI CCCGC 2 cut(s) 120, 1594
FblI GTMKAC 2 cut(s) 775, 932
Fnu4HI GCNGC 6 cut(s) 19, 153, 470, 1227, 1568, 1771
FokI GGATG 6 cut(s) 350, 671, 1178, 1439, 1520, 1811
FriOI GRGCYC 3 cut(s) 13, 704, 1023
Fsp4HI GCNGC 6 cut(s) 19, 153, 470, 1227, 1568, 1771
FspBI CTAG 4 cut(s) 939, 1223, 1233, 1302
GlaI GCGC 2 cut(s) 110, 412
GluI GCNGC 6 cut(s) 19, 153, 470, 1227, 1568, 1771
HaeII RGCGCY 2 cut(s) 112, 414
HaeIII GGCC 4 cut(s) 590, 962, 1401, 1862
HapII CCGG 3 cut(s) 236, 719, 1183
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 111, 413
Hin1I GRCGYC 2 cut(s) 171, 411
Hin1II CATG 6 cut(s) 860, 869, 976, 1424, 1828, 1832
Hin6I GCGC 2 cut(s) 109, 411
HinP1I GCGC 2 cut(s) 109, 411
HincII GTYRAC 2 cut(s) 1578, 1813
HindII GTYRAC 2 cut(s) 1578, 1813
HpaII CCGG 3 cut(s) 236, 719, 1183
HphI GGTGA 6 cut(s) 250, 619, 733, 1322, 1732, 1845
Hpy166II GTNNAC 5 cut(s) 169, 776, 933, 1578, 1813
Hpy188I TCNGA 5 cut(s) 40, 46, 228, 295, 1887
Hpy188III TCNNGA 5 cut(s) 330, 1143, 1217, 1275, 1337
Hpy8I GTNNAC 5 cut(s) 169, 776, 933, 1578, 1813
Hpy99I CGWCG 4 cut(s) 50, 148, 151, 167
HpyAV CCTTC 6 cut(s) 604, 937, 952, 1042, 1198, 1909
HpyCH4III ACNGT 5 cut(s) 560, 930, 1131, 1684, 1817
HpyCH4IV ACGT 2 cut(s) 84, 165
HpyCH4V TGCA 9 cut(s) 155, 445, 797, 1488, 1679, 1828, 1841, 1877, 1936
HpyF10VI GCNNNNNNNGC 8 cut(s) 794, 909, 1027, 1302, 1388, 1767, 1776, 1902
HpyF3I CTNAG 7 cut(s) 426, 594, 709, 1059, 1141, 1629, 1857
HpySE526I ACGT 2 cut(s) 84, 165
Hsp92I GRCGYC 2 cut(s) 171, 411
Hsp92II CATG 6 cut(s) 860, 869, 976, 1424, 1828, 1832
HspAI GCGC 2 cut(s) 109, 411
KasI GGCGCC 1 cut(s) 410
Kzo9I GATC 5 cut(s) 116, 463, 1145, 1347, 1476
LguI GCTCTTC 2 cut(s) 692, 1760
LmnI GCTCC 9 cut(s) 8, 64, 900, 981, 1026, 1124, 1564, 1663, 1901
Lsp1109I GCAGC 4 cut(s) 139, 1238, 1554, 1757
LweI GCATC 3 cut(s) 432, 667, 1273
MaeI CTAG 4 cut(s) 939, 1223, 1233, 1302
MaeII ACGT 2 cut(s) 84, 165
MaeIII GTNAC 5 cut(s) 286, 1054, 1429, 1642, 1843
MalI GATC 5 cut(s) 118, 465, 1147, 1349, 1478
MbiI CCGCTC 1 cut(s) 127
MboI GATC 5 cut(s) 116, 463, 1145, 1347, 1476
MfeI CAATTG 4 cut(s) 446, 1249, 1320, 1925
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 6 cut(s) 13, 704, 1023, 1129, 1460, 1607
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 6 cut(s) 35, 199, 994, 1573, 1796, 1844
MmeI TCCRAC 3 cut(s) 63, 69, 1113
Mph1103I ATGCAT 1 cut(s) 1830
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 3 cut(s) 321, 546, 843
MslI CAYNNNNRTG 3 cut(s) 1094, 1425, 1738
MspA1I CMGCKG 1 cut(s) 469
MspI CCGG 3 cut(s) 236, 719, 1183
MspR9I CCNGG 4 cut(s) 214, 705, 1398, 1586
MunI CAATTG 4 cut(s) 446, 1249, 1320, 1925
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 4 cut(s) 214, 705, 1398, 1586
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 8 cut(s) 794, 909, 1027, 1302, 1388, 1767, 1776, 1902
NarI GGCGCC 1 cut(s) 411
NdeII GATC 5 cut(s) 116, 463, 1145, 1347, 1476
NheI GCTAGC 1 cut(s) 1301
NlaIII CATG 6 cut(s) 860, 869, 976, 1424, 1828, 1832
NlaIV GGNNCC 7 cut(s) 133, 218, 412, 1022, 1137, 1256, 1863
NmeAIII GCCGAG 3 cut(s) 148, 463, 988
NmuCI GTSAC 1 cut(s) 1642
NsiI ATGCAT 1 cut(s) 1830
NspI RCATGY 1 cut(s) 976
OliI CACNNNNGTG 1 cut(s) 1738
PaeR7I CTCGAG 1 cut(s) 29
PceI AGGCCT 1 cut(s) 1401
PciSI GCTCTTC 2 cut(s) 692, 1760
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 4 cut(s) 223, 232, 662, 1589
PflMI CCANNNNNTGG 1 cut(s) 1403
PfoI TCCNGGA 1 cut(s) 212
PinAI ACCGGT 1 cut(s) 718
PkrI GCNGC 6 cut(s) 20, 154, 471, 1228, 1569, 1772
PleI GAGTC 6 cut(s) 35, 198, 993, 1573, 1796, 1844
PluTI GGCGCC 1 cut(s) 414
PpsI GAGTC 6 cut(s) 35, 198, 993, 1573, 1796, 1844
PpuMI RGGWCCY 1 cut(s) 1219
PshAI GACNNNNGTC 1 cut(s) 1927
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 1219
Psp6I CCWGG 4 cut(s) 212, 703, 1396, 1584
PspGI CCWGG 4 cut(s) 212, 703, 1396, 1584
PspN4I GGNNCC 7 cut(s) 133, 218, 412, 1022, 1137, 1256, 1863
PspPI GGNCC 4 cut(s) 1135, 1219, 1238, 1861
PspPPI RGGWCCY 1 cut(s) 1219
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 1 cut(s) 1938
PstNI CAGNNNCTG 1 cut(s) 1847
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 4 cut(s) 200, 248, 1358, 1915
RsaNI GTAC 4 cut(s) 199, 247, 1357, 1914
RseI CAYNNNNRTG 3 cut(s) 1094, 1425, 1738
SacI GAGCTC 1 cut(s) 13
SapI GCTCTTC 2 cut(s) 692, 1760
SaqAI TTAA 3 cut(s) 321, 546, 843
SatI GCNGC 6 cut(s) 19, 153, 470, 1227, 1568, 1771
Sau3AI GATC 5 cut(s) 116, 463, 1145, 1347, 1476
Sau96I GGNCC 4 cut(s) 1135, 1219, 1238, 1861
SchI GAGTC 6 cut(s) 35, 199, 994, 1573, 1796, 1844
ScrFI CCNGG 4 cut(s) 214, 705, 1398, 1586
SduI GDGCHC 6 cut(s) 13, 704, 1023, 1129, 1460, 1607
SfaNI GCATC 3 cut(s) 432, 667, 1273
SfcI CTRYAG 1 cut(s) 1934
SfoI GGCGCC 1 cut(s) 412
Sfr274I CTCGAG 1 cut(s) 29
SgrAI CRCCGGYG 1 cut(s) 718
SinI GGWCC 3 cut(s) 1135, 1219, 1238
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 3 cut(s) 1094, 1425, 1738
SmlI CTYRAG 4 cut(s) 29, 179, 194, 1273
SmoI CTYRAG 4 cut(s) 29, 179, 194, 1273
SseBI AGGCCT 1 cut(s) 1401
SspDI GGCGCC 1 cut(s) 410
SspMI CTAG 4 cut(s) 939, 1223, 1233, 1302
SstI GAGCTC 1 cut(s) 13
StuI AGGCCT 1 cut(s) 1401
StyD4I CCNGG 4 cut(s) 212, 703, 1396, 1584
StyI CCWWGG 5 cut(s) 938, 1258, 1402, 1562, 1794
TaaI ACNGT 5 cut(s) 560, 930, 1131, 1684, 1817
TaiI ACGT 2 cut(s) 87, 168
TaqI TCGA 6 cut(s) 30, 331, 477, 1038, 1148, 1370
TaqII GACCGA 1 cut(s) 655
TatI WGTACW 1 cut(s) 1356
TauI GCSGC 2 cut(s) 21, 472
TfiI GAWTC 4 cut(s) 223, 232, 662, 1589
Tru1I TTAA 3 cut(s) 321, 546, 843
Tru9I TTAA 3 cut(s) 321, 546, 843
TscAI CASTG 5 cut(s) 510, 523, 598, 1298, 1312
TseFI GTSAC 1 cut(s) 1642
TseI GCWGC 4 cut(s) 152, 1226, 1567, 1770
Tsp45I GTSAC 1 cut(s) 1642
TspDTI ATGAA 3 cut(s) 971, 1007, 1813
TspGWI ACGGA 2 cut(s) 1478, 1661
TspRI CASTG 5 cut(s) 510, 523, 598, 1298, 1312
Van91I CCANNNNNTGG 1 cut(s) 1403
VpaK11BI GGWCC 3 cut(s) 1135, 1219, 1238
XapI RAATTY 7 cut(s) 374, 736, 847, 886, 919, 1551, 1691
XceI RCATGY 1 cut(s) 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XhoI CTCGAG 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 938
XmiI GTMKAC 2 cut(s) 775, 932
XmnI GAANNNNTTC 1 cut(s) 512
XspI CTAG 4 cut(s) 939, 1223, 1233, 1302
Zsp2I ATGCAT 1 cut(s) 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.