pycom08g08230

CYPRO4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014939
Physical Location & Seq
Forward (+)
6626046 .. 6627407
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08230.1

Sequence Viewer

Length: 1362 bp
ATGGGCGGAGCTCACAGCCGCGAACACCTCGAGCTCTCCGACTCCGACGAGGAAGAGAGCGATGAGCAGGAGAGCTATGAGGATGTGGAGGAAACTAAAGCCGACCACCGCCAGAGATCCACCGAGCGGAGACCCAAAACGCCGTCGTCGGCGGAGTTAGACAATGTGGACGCCAAGCTCAAGGCCTTGAGTCTCAAATACGGGTCTTCTTCCAAGAACCCGAATCTGAAGAATCCGGTAAAGCTCTACCTTCACATTGGTGGGAACACCCCCAAGGCGAAATGGGTAACTTCCGAAAAACTCACTTCTTACAATTTTATTAAGACATCTCGAATGGAGGTTGATAATGACGAGGAGGTAGAGGAAGAAGAGGAATTTGATGAGGAGGGTTGTTGGGTTTTGAGGGTTGGGGCTAAAGTTAGGGCTAAGGTATCGGCCGAGATGCAATTACTCACTTTCGCCGATCAGCGACGTGTCGATTTCTTTGCCAGAGGTGTGTGGGCAATGAAGTTTTTCGGTGATGAGGAGTATAGGGCTTTTGTGATTAAGTACAATGACTGTTTGTTTGAGAACACTTATGGGTATGAGGCCACTGAGGAGAACAGGGTGAAGGTTTATGGGAAAGATTTTGTTGGGTGGGCGAAGCCCGAAATGGCAGATGATTCGGTGTGGGAAGATGCGGAGGATAGTTTGTTGAAGAGCCCTGGCTCGGCAACACCAGTGAGGGCAAATCAGAATTTGAGAGAGGAGTTTGAGGAGGCCCACGATGGCGGTATACAGAGCTTGGCATTGGGTGCATTGGACAATAGTTTCTTGGTGGGGAACTCTGGCATTCAGGTAGTTAAGAATTTCTCTCATGGAATACATGGTAAAGGTGTTTATGTGAATTTTGATGATGGGAGCTATGGAGGGGGTTCAAATTTGGCACGGTCAACGCCTAGGAAGGCTCTGCTGATGAAGGCCGAGACTAACATGCTCCTTATGAGTCCGATGAATGAGGGGAAGCCTCACACCACAGGGCTCCATCAGCTTGATATTGAAACCGGGAAGGTTGTTACTGAGTGGAAGTTTGAGAAAGATGGGGCTGACATTACAATGAGGGATATCACAAATGACAGTAAGGGAGCGCAGTTGGACCCCTCAGGATCAACATTCTTGGGTCTGGATGACAACAGGCTTTGCCGGTGGGATATGCGTGATAGGAAAGGAATGGTTCAGGACCTTGCGGCTTCTAGTGGACCTGTTCTCAATTGGAACCAAGGGCATCAGTTCTCAAGAGGGACGAACTTTCAGTGCTTTGCTAGCACTGGAGATGGCTCAATTGCTGTTGGGTCGCTTGATGGGAAGATCAGGTTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

454

Amino Acids

50.6

Weight (kDa)

4.96

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 40 - 207 1.1e-76 Domain of unknown function (DUF7135)
VID27 PF08553 311 - 453 1.8e-15 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33400
fragaria_vesca FvH4_2g34470
malus_domestica MD08G1101800.v1.1 MD15G1083300.v1.1
prunus_persica Prupe.1G435800_v2.0.a1
pyrus_communis pycom08g08230 pycom08g08240 pycom15g07780
rosa_chinensis RchiOBHm_Chr6g0307901
rosa_laevigata RLG00000010688
rosa_rugosa Rorug06G0361500
rosa_samantha Rh6AG470600 Rh6BG481600 Rh6CG487000 Rh6DG473100
rosa_wichuraiana Rw6G041090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 775
AccII CGCG 1 cut(s) 21
AciI CCGC 8 cut(s) 6, 19, 109, 127, 152, 680, 771, 1226
AclWI GGATC 2 cut(s) 111, 1153
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 5 cut(s) 374, 736, 847, 886, 919
AcuI CTGAAG 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 2 cut(s) 126, 709
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 3 cut(s) 697, 918, 1040
AjiI CACGTC 1 cut(s) 473
AjnI CCWGG 1 cut(s) 703
AleI CACNNNNGTG 1 cut(s) 258
AluBI AGCT 8 cut(s) 11, 34, 75, 178, 244, 783, 903, 1030
AluI AGCT 8 cut(s) 11, 34, 75, 178, 244, 783, 903, 1030
Alw21I GWGCWC 2 cut(s) 13, 36
Alw26I GTCTC 3 cut(s) 124, 197, 959
AlwI GGATC 2 cut(s) 111, 1153
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 5 cut(s) 183, 435, 588, 759, 960
ApoI RAATTY 5 cut(s) 374, 736, 847, 886, 919
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 512
AspA2I CCTAGG 1 cut(s) 938
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 4 cut(s) 760, 1135, 1219, 1238
AsuC2I CCSGG 1 cut(s) 1045
AsuHPI GGTGA 2 cut(s) 530, 619
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 3 cut(s) 1135, 1219, 1238
AvrII CCTAGG 1 cut(s) 938
AxyI CCTNAGG 1 cut(s) 1141
BanII GRGCYC 4 cut(s) 13, 36, 704, 1023
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 13, 36
BccI CCATC 6 cut(s) 761, 890, 1032, 1073, 1307, 1334
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 4 cut(s) 467, 501, 645, 679
BciT130I CCWGG 1 cut(s) 705
BcnI CCSGG 1 cut(s) 1045
BcoDI GTCTC 3 cut(s) 124, 197, 959
BfaI CTAG 3 cut(s) 939, 1233, 1302
BisI GCNGC 2 cut(s) 19, 1227
BlnI CCTAGG 1 cut(s) 938
BlsI GCNGC 2 cut(s) 20, 1228
Bme1390I CCNGG 2 cut(s) 705, 1045
Bme18I GGWCC 3 cut(s) 1135, 1219, 1238
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 473
BmgT120I GGNCC 4 cut(s) 760, 1135, 1219, 1238
BmiI GGNNCC 3 cut(s) 1022, 1137, 1256
BmrFI CCNGG 2 cut(s) 705, 1045
BmsI GCATC 3 cut(s) 432, 667, 1273
BmtI GCTAGC 1 cut(s) 1305
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 1329
Bpu10I CCTNAGC 1 cut(s) 426
BpuEI CTTGAG 3 cut(s) 164, 208, 1258
BpuMI CCSGG 1 cut(s) 1045
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 124
BsaJI CCNNGG 4 cut(s) 273, 703, 938, 1258
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 709
Bse118I RCCGGY 1 cut(s) 1182
Bse1I ACTGG 2 cut(s) 719, 1312
Bse21I CCTNAGG 1 cut(s) 1141
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 273, 703, 938, 1258
BseGI GGATG 2 cut(s) 88, 1171
BseLI CCNNNNNNNGG 2 cut(s) 126, 709
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 3 cut(s) 585, 1050, 1155
BseNI ACTGG 2 cut(s) 719, 1312
BseRI GAGGAG 6 cut(s) 368, 398, 539, 611, 761, 770
BseX3I CGGCCG 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 5 cut(s) 185, 437, 590, 761, 962
BsiEI CGRYCG 1 cut(s) 438
BsiHKAI GWGCWC 2 cut(s) 13, 36
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 236, 1044, 1183
BslFI GGGAC 1 cut(s) 1294
BslI CCNNNNNNNGG 2 cut(s) 126, 709
BsmAI GTCTC 3 cut(s) 124, 197, 959
BsmFI GGGAC 1 cut(s) 1294
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 5 cut(s) 185, 437, 590, 761, 962
Bso31I GGTCTC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 4 cut(s) 13, 36, 704, 1023
Bsp143I GATC 4 cut(s) 116, 463, 1145, 1347
BspACI CCGC 8 cut(s) 6, 19, 109, 127, 152, 680, 771, 1226
BspANI GGCC 5 cut(s) 185, 437, 590, 761, 962
BspCNI CTCAG 3 cut(s) 586, 1051, 1154
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 1022, 1137, 1256
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 2 cut(s) 111, 1153
BspQI GCTCTTC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 1182
BsrI ACTGG 2 cut(s) 719, 1312
BssAI RCCGGY 1 cut(s) 1182
BssECI CCNNGG 4 cut(s) 273, 703, 938, 1258
BssMI GATC 4 cut(s) 116, 463, 1145, 1347
BssNAI GTATAC 1 cut(s) 776
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 3 cut(s) 273, 938, 1258
Bst1107I GTATAC 1 cut(s) 776
Bst2UI CCWGG 1 cut(s) 705
Bst4CI ACNGT 3 cut(s) 560, 930, 1118
Bst6I CTCTTC 3 cut(s) 48, 363, 692
BstACI GRCGYC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 1 cut(s) 1303
BstDEI CTNAG 4 cut(s) 426, 594, 1059, 1141
BstF5I GGATG 2 cut(s) 88, 1171
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 4 cut(s) 119, 466, 1148, 1350
BstMAI GTCTC 3 cut(s) 124, 197, 959
BstMBI GATC 4 cut(s) 116, 463, 1145, 1347
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 3 cut(s) 794, 1027, 1302
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 1 cut(s) 976
BstSCI CCNGG 2 cut(s) 703, 1043
BstUI CGCG 1 cut(s) 21
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BstZ17I GTATAC 1 cut(s) 776
BstZI CGGCCG 1 cut(s) 435
Bsu36I CCTNAGG 1 cut(s) 1141
BsuRI GGCC 5 cut(s) 185, 437, 590, 761, 962
BtgZI GCGATG 1 cut(s) 75
BtrI CACGTC 1 cut(s) 473
BtsCI GGATG 2 cut(s) 88, 1171
BtsIMutI CAGTG 4 cut(s) 591, 726, 1298, 1305
Cac8I GCNNGC 1 cut(s) 1303
CfoI GCGC 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 1182
Cfr13I GGNCC 4 cut(s) 760, 1135, 1219, 1238
CseI GACGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 550
CviAII CATG 3 cut(s) 857, 866, 973
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 4 cut(s) 426, 594, 1059, 1141
DpnI GATC 4 cut(s) 118, 465, 1147, 1349
DpnII GATC 4 cut(s) 116, 463, 1145, 1347
EaeI YGGCCR 1 cut(s) 435
EagI CGGCCG 1 cut(s) 435
Eam1104I CTCTTC 3 cut(s) 48, 363, 692
EarI CTCTTC 3 cut(s) 48, 363, 692
EciI GGCGGA 2 cut(s) 21, 167
Ecl136II GAGCTC 2 cut(s) 11, 34
EclXI CGGCCG 1 cut(s) 435
Eco130I CCWWGG 3 cut(s) 273, 938, 1258
Eco147I AGGCCT 1 cut(s) 185
Eco24I GRGCYC 4 cut(s) 13, 36, 704, 1023
Eco31I GGTCTC 1 cut(s) 124
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 3 cut(s) 1135, 1219, 1238
Eco52I CGGCCG 1 cut(s) 435
Eco53kI GAGCTC 2 cut(s) 11, 34
Eco57I CTGAAG 1 cut(s) 248
Eco81I CCTNAGG 1 cut(s) 1141
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 2 cut(s) 11, 34
EcoO109I RGGNCCY 1 cut(s) 1219
EcoRII CCWGG 1 cut(s) 703
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 3 cut(s) 273, 938, 1258
EcoT38I GRGCYC 4 cut(s) 13, 36, 704, 1023
ErhI CCWWGG 3 cut(s) 273, 938, 1258
FaeI CATG 3 cut(s) 860, 869, 976
FaqI GGGAC 1 cut(s) 1294
FatI CATG 3 cut(s) 856, 865, 972
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 19, 1227
FokI GGATG 2 cut(s) 95, 1178
FriOI GRGCYC 4 cut(s) 13, 36, 704, 1023
Fsp4HI GCNGC 2 cut(s) 19, 1227
FspBI CTAG 3 cut(s) 939, 1233, 1302
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 2 cut(s) 19, 1227
GsuI CTGGAG 1 cut(s) 1329
HaeIII GGCC 5 cut(s) 185, 437, 590, 761, 962
HapII CCGG 3 cut(s) 236, 1044, 1183
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 1129
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 860, 869, 976
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 1 cut(s) 933
HindII GTYRAC 1 cut(s) 933
HinfI GANTC 6 cut(s) 41, 190, 223, 232, 662, 985
HpaII CCGG 3 cut(s) 236, 1044, 1183
HphI GGTGA 2 cut(s) 530, 619
Hpy166II GTNNAC 4 cut(s) 169, 776, 933, 1238
Hpy188I TCNGA 6 cut(s) 40, 46, 228, 295, 735, 990
Hpy188III TCNNGA 5 cut(s) 330, 1143, 1163, 1217, 1275
Hpy8I GTNNAC 4 cut(s) 169, 776, 933, 1238
Hpy99I CGWCG 4 cut(s) 50, 148, 151, 474
HpyAV CCTTC 5 cut(s) 260, 604, 937, 952, 1042
HpyCH4III ACNGT 3 cut(s) 560, 930, 1118
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 2 cut(s) 445, 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 794, 1027, 1302
HpyF3I CTNAG 4 cut(s) 426, 594, 1059, 1141
HpySE526I ACGT 1 cut(s) 472
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 860, 869, 976
HspAI GCGC 1 cut(s) 1127
Kzo9I GATC 4 cut(s) 116, 463, 1145, 1347
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 5 cut(s) 8, 900, 981, 1026, 1124
LweI GCATC 3 cut(s) 432, 667, 1273
MaeI CTAG 3 cut(s) 939, 1233, 1302
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 2 cut(s) 286, 1054
MalI GATC 4 cut(s) 118, 465, 1147, 1349
MbiI CCGCTC 1 cut(s) 127
MboI GATC 4 cut(s) 116, 463, 1145, 1347
MboII GAAGA 9 cut(s) 65, 198, 201, 241, 377, 380, 686, 709, 1357
MfeI CAATTG 2 cut(s) 1249, 1320
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 4 cut(s) 13, 36, 704, 1023
MluCI AATT 9 cut(s) 313, 374, 446, 736, 847, 886, 919, 1249, 1320
MlyI GAGTC 3 cut(s) 35, 199, 994
MmeI TCCRAC 3 cut(s) 63, 69, 1113
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 3 cut(s) 321, 546, 843
MslI CAYNNNNRTG 2 cut(s) 258, 1094
MspI CCGG 3 cut(s) 236, 1044, 1183
MspR9I CCNGG 2 cut(s) 705, 1045
MunI CAATTG 2 cut(s) 1249, 1320
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 705
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 794, 1027, 1302
NciI CCSGG 1 cut(s) 1045
NdeII GATC 4 cut(s) 116, 463, 1145, 1347
NheI GCTAGC 1 cut(s) 1301
NlaIII CATG 3 cut(s) 860, 869, 976
NlaIV GGNNCC 3 cut(s) 1022, 1137, 1256
NmeAIII GCCGAG 3 cut(s) 463, 689, 988
NspI RCATGY 1 cut(s) 976
OliI CACNNNNGTG 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 29
PceI AGGCCT 1 cut(s) 185
PciSI GCTCTTC 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 3 cut(s) 223, 232, 662
PkrI GCNGC 2 cut(s) 20, 1228
PleI GAGTC 3 cut(s) 35, 198, 993
PpsI GAGTC 3 cut(s) 35, 198, 993
PpuMI RGGWCCY 1 cut(s) 1219
Psp124BI GAGCTC 2 cut(s) 13, 36
Psp5II RGGWCCY 1 cut(s) 1219
Psp6I CCWGG 1 cut(s) 703
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 3 cut(s) 1022, 1137, 1256
PspPI GGNCC 4 cut(s) 760, 1135, 1219, 1238
PspPPI RGGWCCY 1 cut(s) 1219
PspXI VCTCGAGB 1 cut(s) 29
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 2 cut(s) 258, 1094
SacI GAGCTC 2 cut(s) 13, 36
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 3 cut(s) 321, 546, 843
SatI GCNGC 2 cut(s) 19, 1227
Sau3AI GATC 4 cut(s) 116, 463, 1145, 1347
Sau96I GGNCC 4 cut(s) 760, 1135, 1219, 1238
SchI GAGTC 3 cut(s) 35, 199, 994
ScrFI CCNGG 2 cut(s) 705, 1045
SduI GDGCHC 4 cut(s) 13, 36, 704, 1023
SfaNI GCATC 3 cut(s) 432, 667, 1273
Sfr274I CTCGAG 1 cut(s) 29
SinI GGWCC 3 cut(s) 1135, 1219, 1238
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 2 cut(s) 258, 1094
SmlI CTYRAG 4 cut(s) 29, 179, 187, 1273
SmoI CTYRAG 4 cut(s) 29, 179, 187, 1273
Sse9I AATT 9 cut(s) 313, 374, 446, 736, 847, 886, 919, 1249, 1320
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 8 cut(s) 6, 19, 109, 127, 152, 680, 771, 1226
SspMI CTAG 3 cut(s) 939, 1233, 1302
SstI GAGCTC 2 cut(s) 13, 36
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 703, 1043
StyI CCWWGG 3 cut(s) 273, 938, 1258
TaaI ACNGT 3 cut(s) 560, 930, 1118
TaiI ACGT 1 cut(s) 475
TaqI TCGA 3 cut(s) 30, 331, 477
TasI AATT 9 cut(s) 313, 374, 446, 736, 847, 886, 919, 1249, 1320
TatI WGTACW 1 cut(s) 549
TauI GCSGC 2 cut(s) 21, 1229
TfiI GAWTC 3 cut(s) 223, 232, 662
Tru1I TTAA 3 cut(s) 321, 546, 843
Tru9I TTAA 3 cut(s) 321, 546, 843
TscAI CASTG 4 cut(s) 598, 726, 1298, 1312
TspDTI ATGAA 3 cut(s) 521, 971, 1007
TspRI CASTG 4 cut(s) 598, 726, 1298, 1312
VpaK11BI GGWCC 3 cut(s) 1135, 1219, 1238
XapI RAATTY 5 cut(s) 374, 736, 847, 886, 919
XceI RCATGY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 938
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 512
XspI CTAG 3 cut(s) 939, 1233, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.