MD08G1101800.v1.1

CYPRO4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014939
Physical Location & Seq
Forward (+)
8550059 .. 8552846
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1101800.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGGCGGAGCTCACAGCCGTGAACACCTCGAGCTCTCCGACTCCGACGAGGAAGAGAGCGAGGAGCAGGAGAGCTATGAGGATGTGGAGGAAACTGAAGCCGACCAACGTCAGAGATCCGCCGATCGGGGACCCAAAACGCCGTCGTCGGCGGAGTTAGACGATGTGGACGCAAAGCTCAAGGCTTTGAGTCTCAAATACGGGTCTTCTTCCAAGAACCCGAATCTGAAGAATCCGGTGAAGCTTTACGTTCACATTGGTGGGAACACCCCCAAGGCGAAATGGGTAACTTCCGAGAAACTCACTTCTTACAATTTTATTAAGACATCTCGAATGGAGGTTGATAATGACGAGGAGGTAGAGGAAGAAGAGGAATTTGATGAGGAGGGTTGTTGGGTTTTGAGGGTTGGGGCTAAAGTTAGGGCTAAGGTATCGGCCGAGATGCAATTGCTCACTTTCGCCGATCAGCGGCGTGTCGATTTCTTTGCCAGAGGTGTGTGGGCAATGAAGTTTTTCGGTGATGAGGAGTATAGGGCTTTTGTGCTTAAGTACAATGACTGTTTGTTTGAGAACACTTATGGGTATGAGGCCACCGAGGAGAACAGGGTGAAGGTTTATGGGAAGGATTTTGTCGGGTGGGCGAAGCCCGAAATGGCGGATGATTCGGTGTGGGAAGATGCGGAGGATAGTTTGTTGAAGAGCCCTGGCTCGGCAACACCAGTGAGGGCAAATCAGAATTTGAGAGAGGAGTTTGAGGAGGCCCACGATGGCGGGATACAGAGCTTGGCATTGGGTGCATTGGACAATAGTTTCTTGGTGGGGAACTCTGGCATTCAGGTAGTTAAGAATTTCTCTCATGGAATTCATGGTAAAGGTGTTTATGTGAATTTTGATGATGGGAGCTATGGAGGGGGTTCGAATTTGGCACGGTCAACGCCTAGGAAGGCTCTGCTGATGAAGGCCGAGACTAACATGCTCCTTATGAGTCCGATGAATGAGGGGAAGCCTCACACAACAGGGCTTCATCAGCTTGATATTGAAACCGGGAAGGTTGTTACTGAGTGGAAGTTTGAGAAAGATGGGGCTGACATTACAATGAGGGATATCACAAATGACAGTAAGGGAGCGCAGTTGGACCCCTCAGGATCAACATTCTTGGGTCTGGATGACAACAGGCTTTGCCGGTGGGATATGCGTGATAGGAAAGGAATGGTTCAGGACCTTGCGGCTTCTAGTGGACCTGTTCTCAATTGGAACCAAGGGCATCAGTTCTCAAGAGGGACTAACTTTCAGTGCTTTGCTAGCACTGGAGATGGCTCAATTGCTGTTGGGTCTCTTGATGGGAAGATCAGGTTGTATTCAATCAATTCGATGAGGCAGGCGAAAACTGCTTTTCCAGGCCTTGGTTCACCTATCACTCATGTGGATGTTACTTTTGATGGGAAGTGGATTTTGGGCACAACCGACACTTATTTAATCCTCATCTGCACAATCTTTACTGATAAGGATGGCAAGACAAAGACTGGTTTTAGTGGGCGTATGGGAAATAGAATTTCAGCTCCAAGACTGCTGAAGCTGACTCCCCTGGATTCACATCTGGCGGGCATGAATAACAAGTTTCGTAACGCTCAGTTTTCGTGGGTCACAGAGAATGGGAAGCAGGAGCGTCATCTGGTTGCTACTGTGGGCAAATTTAGTGTGGTTTGGAACTTTCAACAGGTGAAGGATGGCTCGCATGAGTGCTACCGCAATCAGCAGGGCTTGAAGAGCTGCTACTGCTACAAGATAGTCCCTAAGGACGACTCCATTGTCGACAGTCGCTTCATGCATGACAAATTTGCAGTTACCGACTCGCCTGAGGCTCCACTTGTGATTGCAACCCCTCTCAAAGTCAGCTCCTTCAGCATATCCAGTACTAATAGACAATTGTACTCGGAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

648

Amino Acids

72.15

Weight (kDa)

5.31

Isoelectric Point (pI)

29.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 42 - 207 6.6e-76 Domain of unknown function (DUF7135)
VID27 PF08553 311 - 583 4.9e-44 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33400
fragaria_vesca FvH4_2g34470
malus_domestica MD08G1101800.v1.1 MD15G1083300.v1.1
prunus_persica Prupe.1G435800_v2.0.a1
pyrus_communis pycom08g08230 pycom08g08240 pycom15g07780
rosa_chinensis RchiOBHm_Chr6g0307901
rosa_laevigata RLG00000010688
rosa_rugosa Rorug06G0361500
rosa_samantha Rh6AG470600 Rh6BG481600 Rh6CG487000 Rh6DG473100
rosa_wichuraiana Rw6G041090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1808
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccI GTMKAC 1 cut(s) 1812
AclWI GGATC 2 cut(s) 111, 1153
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 9 cut(s) 374, 736, 847, 861, 886, 919, 1551, 1691, 1835
AcuI CTGAAG 4 cut(s) 117, 248, 1592, 1885
AfaI GTAC 3 cut(s) 551, 1915, 1931
AfiI CCNNNNNNNGG 4 cut(s) 126, 468, 709, 1403
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 5 cut(s) 697, 1040, 1362, 1715, 1765
AjnI CCWGG 3 cut(s) 703, 1396, 1584
AjuI GAANNNNNNNTTGG 2 cut(s) 1436, 1468
AleI CACNNNNGTG 3 cut(s) 18, 258, 1421
Alw21I GWGCWC 2 cut(s) 13, 36
Alw26I GTCTC 3 cut(s) 197, 959, 1338
AlwI GGATC 2 cut(s) 111, 1153
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 5 cut(s) 435, 588, 759, 960, 1399
ApeKI GCWGC 1 cut(s) 1770
ApoI RAATTY 9 cut(s) 374, 736, 847, 861, 886, 919, 1551, 1691, 1835
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 512
AspA2I CCTAGG 1 cut(s) 938
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 5 cut(s) 131, 760, 1135, 1219, 1238
AsuC2I CCSGG 1 cut(s) 1045
AsuHPI GGTGA 5 cut(s) 250, 530, 619, 1401, 1732
AsuII TTCGAA 1 cut(s) 917
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 4 cut(s) 131, 1135, 1219, 1238
AvrII CCTAGG 1 cut(s) 938
AxyI CCTNAGG 3 cut(s) 1141, 1794, 1857
BaeGI GKGCMC 1 cut(s) 1460
BanII GRGCYC 3 cut(s) 13, 36, 704
BarI GAAGNNNNNNTAC 2 cut(s) 1757, 1789
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 13, 36
BbvI GCAGC 1 cut(s) 1757
BccI CCATC 8 cut(s) 761, 890, 1073, 1307, 1334, 1433, 1502, 1721
BceAI ACGGC 2 cut(s) 3, 127
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 3 cut(s) 705, 1398, 1586
BciVI GTATCC 1 cut(s) 768
BcnI CCSGG 1 cut(s) 1045
BcoDI GTCTC 3 cut(s) 197, 959, 1338
BfaI CTAG 3 cut(s) 939, 1233, 1302
BfrI CTTAAG 1 cut(s) 545
BfuI GTATCC 1 cut(s) 768
BisI GCNGC 3 cut(s) 470, 1227, 1771
BlnI CCTAGG 1 cut(s) 938
BlsI GCNGC 3 cut(s) 471, 1228, 1772
BmcAI AGTACT 1 cut(s) 1915
Bme1390I CCNGG 4 cut(s) 705, 1045, 1398, 1586
Bme18I GGWCC 4 cut(s) 131, 1135, 1219, 1238
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 5 cut(s) 131, 760, 1135, 1219, 1238
BmiI GGNNCC 5 cut(s) 132, 133, 1137, 1256, 1863
BmrFI CCNGG 4 cut(s) 705, 1045, 1398, 1586
BmsI GCATC 3 cut(s) 432, 667, 1273
BmtI GCTAGC 1 cut(s) 1305
BoxI GACNNNNGTC 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 1329
Bpu10I CCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 917
BpuEI CTTGAG 2 cut(s) 164, 1258
BpuMI CCSGG 1 cut(s) 1045
BsaBI GATNNNNATC 1 cut(s) 1593
BsaI GGTCTC 1 cut(s) 1338
BsaJI CCNNGG 7 cut(s) 273, 594, 703, 938, 1258, 1402, 1584
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 468, 709, 1403
Bse118I RCCGGY 1 cut(s) 1182
Bse1I ACTGG 4 cut(s) 719, 1312, 1528, 1911
Bse21I CCTNAGG 3 cut(s) 1141, 1794, 1857
Bse3DI GCAATG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 1593
BseBI CCWGG 3 cut(s) 705, 1398, 1586
BseDI CCNNGG 7 cut(s) 273, 594, 703, 938, 1258, 1402, 1584
BseGI GGATG 6 cut(s) 88, 664, 1171, 1432, 1513, 1732
BseJI GATNNNNATC 1 cut(s) 1593
BseLI CCNNNNNNNGG 4 cut(s) 126, 468, 709, 1403
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 4 cut(s) 1050, 1155, 1643, 1848
BseNI ACTGG 4 cut(s) 719, 1312, 1528, 1911
BseRI GAGGAG 7 cut(s) 77, 368, 398, 539, 611, 761, 770
BseSI GKGCMC 1 cut(s) 1460
BseX3I CGGCCG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 1757
BsgI GTGCAG 1 cut(s) 1471
Bsh1285I CGRYCG 2 cut(s) 127, 438
BshFI GGCC 5 cut(s) 437, 590, 761, 962, 1401
BsiEI CGRYCG 2 cut(s) 127, 438
BsiHKAI GWGCWC 2 cut(s) 13, 36
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 236, 1044, 1183
BslFI GGGAC 3 cut(s) 144, 1294, 1775
BslI CCNNNNNNNGG 4 cut(s) 126, 468, 709, 1403
BsmAI GTCTC 3 cut(s) 197, 959, 1338
BsmFI GGGAC 3 cut(s) 144, 1294, 1775
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 5 cut(s) 437, 590, 761, 962, 1401
Bso31I GGTCTC 1 cut(s) 1338
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 917
Bsp1286I GDGCHC 4 cut(s) 13, 36, 704, 1460
Bsp143I GATC 5 cut(s) 116, 124, 463, 1145, 1347
BspANI GGCC 5 cut(s) 437, 590, 761, 962, 1401
BspCNI CTCAG 4 cut(s) 1051, 1154, 1642, 1849
BspLI GGNNCC 5 cut(s) 132, 133, 1137, 1256, 1863
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 2 cut(s) 111, 1153
BspQI GCTCTTC 2 cut(s) 692, 1760
BspT104I TTCGAA 1 cut(s) 917
BspTI CTTAAG 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 1338
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 1182
BsrI ACTGG 4 cut(s) 719, 1312, 1528, 1911
BssAI RCCGGY 1 cut(s) 1182
BssECI CCNNGG 7 cut(s) 273, 594, 703, 938, 1258, 1402, 1584
BssMI GATC 5 cut(s) 116, 124, 463, 1145, 1347
BssT1I CCWWGG 4 cut(s) 273, 938, 1258, 1402
Bst2UI CCWGG 3 cut(s) 705, 1398, 1586
Bst4CI ACNGT 5 cut(s) 560, 930, 1118, 1684, 1817
Bst6I CTCTTC 4 cut(s) 48, 363, 692, 1760
BstAFI CTTAAG 1 cut(s) 545
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 917
BstC8I GCNNGC 4 cut(s) 1303, 1380, 1603, 1733
BstDEI CTNAG 6 cut(s) 426, 1059, 1141, 1629, 1794, 1857
BstF5I GGATG 6 cut(s) 88, 664, 1171, 1432, 1513, 1732
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 5 cut(s) 119, 127, 466, 1148, 1350
BstMAI GTCTC 3 cut(s) 197, 959, 1338
BstMBI GATC 5 cut(s) 116, 124, 463, 1145, 1347
BstMCI CGRYCG 2 cut(s) 127, 438
BstMWI GCNNNNNNNGC 7 cut(s) 794, 1027, 1302, 1388, 1767, 1776, 1902
BstNI CCWGG 3 cut(s) 705, 1398, 1586
BstNSI RCATGY 1 cut(s) 976
BstPAI GACNNNNGTC 1 cut(s) 108
BstSCI CCNGG 4 cut(s) 703, 1043, 1396, 1584
BstSLI GKGCMC 1 cut(s) 1460
BstV1I GCAGC 1 cut(s) 1757
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BstZI CGGCCG 1 cut(s) 435
Bsu36I CCTNAGG 3 cut(s) 1141, 1794, 1857
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 5 cut(s) 437, 590, 761, 962, 1401
BtsCI GGATG 6 cut(s) 88, 664, 1171, 1432, 1513, 1732
BtsIMutI CAGTG 3 cut(s) 726, 1298, 1305
Cac8I GCNNGC 4 cut(s) 1303, 1380, 1603, 1733
CfoI GCGC 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 1182
Cfr13I GGNCC 5 cut(s) 131, 760, 1135, 1219, 1238
CseI GACGC 2 cut(s) 179, 1655
Csp6I GTAC 3 cut(s) 550, 1914, 1930
CviAII CATG 8 cut(s) 857, 866, 973, 1421, 1606, 1736, 1825, 1829
CviQI GTAC 3 cut(s) 550, 1914, 1930
DdeI CTNAG 6 cut(s) 426, 1059, 1141, 1629, 1794, 1857
DpnI GATC 5 cut(s) 118, 126, 465, 1147, 1349
DpnII GATC 5 cut(s) 116, 124, 463, 1145, 1347
DrdI GACNNNNNNGTC 1 cut(s) 1808
DseDI GACNNNNNNGTC 1 cut(s) 1808
EaeI YGGCCR 1 cut(s) 435
EagI CGGCCG 1 cut(s) 435
Eam1104I CTCTTC 4 cut(s) 48, 363, 692, 1760
EarI CTCTTC 4 cut(s) 48, 363, 692, 1760
EciI GGCGGA 4 cut(s) 21, 109, 167, 671
Ecl136II GAGCTC 2 cut(s) 11, 34
EclXI CGGCCG 1 cut(s) 435
Eco130I CCWWGG 4 cut(s) 273, 938, 1258, 1402
Eco147I AGGCCT 1 cut(s) 1401
Eco24I GRGCYC 3 cut(s) 13, 36, 704
Eco31I GGTCTC 1 cut(s) 1338
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 4 cut(s) 131, 1135, 1219, 1238
Eco52I CGGCCG 1 cut(s) 435
Eco53kI GAGCTC 2 cut(s) 11, 34
Eco57I CTGAAG 4 cut(s) 117, 248, 1592, 1885
Eco81I CCTNAGG 3 cut(s) 1141, 1794, 1857
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 2 cut(s) 11, 34
EcoO109I RGGNCCY 2 cut(s) 131, 1219
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 3 cut(s) 703, 1396, 1584
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 4 cut(s) 273, 938, 1258, 1402
EcoT22I ATGCAT 1 cut(s) 1830
EcoT38I GRGCYC 3 cut(s) 13, 36, 704
ErhI CCWWGG 4 cut(s) 273, 938, 1258, 1402
FaeI CATG 8 cut(s) 860, 869, 976, 1424, 1609, 1739, 1828, 1832
FaqI GGGAC 3 cut(s) 144, 1294, 1775
FatI CATG 8 cut(s) 856, 865, 972, 1420, 1605, 1735, 1824, 1828
FauI CCCGC 2 cut(s) 764, 1594
FblI GTMKAC 1 cut(s) 1812
Fnu4HI GCNGC 3 cut(s) 470, 1227, 1771
FokI GGATG 6 cut(s) 95, 671, 1178, 1439, 1520, 1739
FriOI GRGCYC 3 cut(s) 13, 36, 704
Fsp4HI GCNGC 3 cut(s) 470, 1227, 1771
FspBI CTAG 3 cut(s) 939, 1233, 1302
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 3 cut(s) 470, 1227, 1771
GsuI CTGGAG 1 cut(s) 1329
HaeIII GGCC 5 cut(s) 437, 590, 761, 962, 1401
HapII CCGG 3 cut(s) 236, 1044, 1183
HgaI GACGC 2 cut(s) 179, 1655
HhaI GCGC 1 cut(s) 1129
Hin1II CATG 8 cut(s) 860, 869, 976, 1424, 1609, 1739, 1828, 1832
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 2 cut(s) 933, 1813
HindII GTYRAC 2 cut(s) 933, 1813
HindIII AAGCTT 1 cut(s) 242
HpaII CCGG 3 cut(s) 236, 1044, 1183
HphI GGTGA 5 cut(s) 250, 530, 619, 1401, 1732
Hpy166II GTNNAC 7 cut(s) 23, 169, 253, 933, 1238, 1409, 1813
Hpy188I TCNGA 8 cut(s) 40, 46, 114, 228, 295, 735, 990, 1936
Hpy188III TCNNGA 6 cut(s) 330, 1143, 1163, 1217, 1275, 1337
Hpy8I GTNNAC 7 cut(s) 23, 169, 253, 933, 1238, 1409, 1813
Hpy99I CGWCG 3 cut(s) 50, 148, 151
HpyAV CCTTC 7 cut(s) 604, 616, 937, 952, 1042, 1717, 1909
HpyCH4III ACNGT 5 cut(s) 560, 930, 1118, 1684, 1817
HpyCH4IV ACGT 2 cut(s) 109, 249
HpyCH4V TGCA 6 cut(s) 445, 797, 1488, 1828, 1841, 1877
HpyF10VI GCNNNNNNNGC 7 cut(s) 794, 1027, 1302, 1388, 1767, 1776, 1902
HpyF3I CTNAG 6 cut(s) 426, 1059, 1141, 1629, 1794, 1857
HpySE526I ACGT 2 cut(s) 109, 249
Hsp92II CATG 8 cut(s) 860, 869, 976, 1424, 1609, 1739, 1828, 1832
HspAI GCGC 1 cut(s) 1127
KflI GGGWCCC 1 cut(s) 131
Kzo9I GATC 5 cut(s) 116, 124, 463, 1145, 1347
LguI GCTCTTC 2 cut(s) 692, 1760
LmnI GCTCC 9 cut(s) 8, 64, 900, 981, 1124, 1564, 1663, 1867, 1901
Lsp1109I GCAGC 1 cut(s) 1757
LweI GCATC 3 cut(s) 432, 667, 1273
MaeI CTAG 3 cut(s) 939, 1233, 1302
MaeII ACGT 2 cut(s) 109, 249
MaeIII GTNAC 6 cut(s) 286, 1054, 1429, 1622, 1642, 1843
MalI GATC 5 cut(s) 118, 126, 465, 1147, 1349
MboI GATC 5 cut(s) 116, 124, 463, 1145, 1347
MfeI CAATTG 4 cut(s) 446, 1249, 1320, 1925
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 4 cut(s) 13, 36, 704, 1460
MlyI GAGTC 6 cut(s) 35, 199, 994, 1573, 1796, 1844
MmeI TCCRAC 3 cut(s) 63, 69, 1113
Mph1103I ATGCAT 1 cut(s) 1830
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 4 cut(s) 321, 546, 843, 1475
MslI CAYNNNNRTG 5 cut(s) 18, 258, 1094, 1421, 1425
MspA1I CMGCKG 1 cut(s) 469
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 3 cut(s) 236, 1044, 1183
MspR9I CCNGG 4 cut(s) 705, 1045, 1398, 1586
MunI CAATTG 4 cut(s) 446, 1249, 1320, 1925
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 3 cut(s) 705, 1398, 1586
MwoI GCNNNNNNNGC 7 cut(s) 794, 1027, 1302, 1388, 1767, 1776, 1902
NciI CCSGG 1 cut(s) 1045
NdeII GATC 5 cut(s) 116, 124, 463, 1145, 1347
NheI GCTAGC 1 cut(s) 1301
NlaIII CATG 8 cut(s) 860, 869, 976, 1424, 1609, 1739, 1828, 1832
NlaIV GGNNCC 5 cut(s) 132, 133, 1137, 1256, 1863
NmeAIII GCCGAG 3 cut(s) 463, 689, 988
NmuCI GTSAC 1 cut(s) 1642
NsiI ATGCAT 1 cut(s) 1830
NspI RCATGY 1 cut(s) 976
NspV TTCGAA 1 cut(s) 917
OliI CACNNNNGTG 3 cut(s) 18, 258, 1421
PaeR7I CTCGAG 1 cut(s) 29
PceI AGGCCT 1 cut(s) 1401
PciSI GCTCTTC 2 cut(s) 692, 1760
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 4 cut(s) 223, 232, 662, 1589
PflMI CCANNNNNTGG 1 cut(s) 1403
PkrI GCNGC 3 cut(s) 471, 1228, 1772
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 6 cut(s) 35, 198, 993, 1573, 1796, 1844
PpsI GAGTC 6 cut(s) 35, 198, 993, 1573, 1796, 1844
PpuMI RGGWCCY 2 cut(s) 131, 1219
PshAI GACNNNNGTC 1 cut(s) 108
Psp124BI GAGCTC 2 cut(s) 13, 36
Psp5II RGGWCCY 2 cut(s) 131, 1219
Psp6I CCWGG 3 cut(s) 703, 1396, 1584
PspGI CCWGG 3 cut(s) 703, 1396, 1584
PspN4I GGNNCC 5 cut(s) 132, 133, 1137, 1256, 1863
PspPI GGNCC 5 cut(s) 131, 760, 1135, 1219, 1238
PspPPI RGGWCCY 2 cut(s) 131, 1219
PspXI VCTCGAGB 1 cut(s) 29
PsuI RGATCY 1 cut(s) 116
PvuI CGATCG 1 cut(s) 127
RsaI GTAC 3 cut(s) 551, 1915, 1931
RsaNI GTAC 3 cut(s) 550, 1914, 1930
RseI CAYNNNNRTG 5 cut(s) 18, 258, 1094, 1421, 1425
SacI GAGCTC 2 cut(s) 13, 36
SalI GTCGAC 1 cut(s) 1811
SapI GCTCTTC 2 cut(s) 692, 1760
SaqAI TTAA 4 cut(s) 321, 546, 843, 1475
SatI GCNGC 3 cut(s) 470, 1227, 1771
Sau3AI GATC 5 cut(s) 116, 124, 463, 1145, 1347
Sau96I GGNCC 5 cut(s) 131, 760, 1135, 1219, 1238
ScaI AGTACT 1 cut(s) 1915
SchI GAGTC 6 cut(s) 35, 199, 994, 1573, 1796, 1844
ScrFI CCNGG 4 cut(s) 705, 1045, 1398, 1586
SduI GDGCHC 4 cut(s) 13, 36, 704, 1460
SfaNI GCATC 3 cut(s) 432, 667, 1273
Sfr274I CTCGAG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 917
SinI GGWCC 4 cut(s) 131, 1135, 1219, 1238
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 5 cut(s) 18, 258, 1094, 1421, 1425
SmlI CTYRAG 4 cut(s) 29, 179, 545, 1273
SmoI CTYRAG 4 cut(s) 29, 179, 545, 1273
SseBI AGGCCT 1 cut(s) 1401
SspMI CTAG 3 cut(s) 939, 1233, 1302
SstI GAGCTC 2 cut(s) 13, 36
StuI AGGCCT 1 cut(s) 1401
StyD4I CCNGG 4 cut(s) 703, 1043, 1396, 1584
StyI CCWWGG 4 cut(s) 273, 938, 1258, 1402
TaaI ACNGT 5 cut(s) 560, 930, 1118, 1684, 1817
TaiI ACGT 2 cut(s) 112, 252
TaqI TCGA 6 cut(s) 30, 331, 477, 917, 1370, 1812
TatI WGTACW 3 cut(s) 549, 1913, 1929
TauI GCSGC 2 cut(s) 472, 1229
TfiI GAWTC 4 cut(s) 223, 232, 662, 1589
Tru1I TTAA 4 cut(s) 321, 546, 843, 1475
Tru9I TTAA 4 cut(s) 321, 546, 843, 1475
TscAI CASTG 3 cut(s) 726, 1298, 1312
TseFI GTSAC 1 cut(s) 1642
TseI GCWGC 1 cut(s) 1770
Tsp45I GTSAC 1 cut(s) 1642
TspDTI ATGAA 7 cut(s) 521, 854, 971, 1007, 1013, 1622, 1813
TspRI CASTG 3 cut(s) 726, 1298, 1312
Van91I CCANNNNNTGG 1 cut(s) 1403
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 4 cut(s) 131, 1135, 1219, 1238
XapI RAATTY 9 cut(s) 374, 736, 847, 861, 886, 919, 1551, 1691, 1835
XceI RCATGY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 938
XmiI GTMKAC 1 cut(s) 1812
XmnI GAANNNNTTC 1 cut(s) 512
XspI CTAG 3 cut(s) 939, 1233, 1302
ZrmI AGTACT 1 cut(s) 1915
Zsp2I ATGCAT 1 cut(s) 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.