Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0016067

Physical Location & Seq

Genomic Coordinates Forward (+)

28882893 .. 28885347

Gene Structure 2455 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g40541.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGAGAGACAGGATGTTTTCCGACGACACCAGCTTACCTAGGGCGAGTAATTTCGGCAACGGCGTCCATTCCGATCCCAATATATCGGCCAACGCTATGTCCCCGGACGTCCTTCACGAATACAAAATGCGAAACAGTAGCGCGTCGCCTTTCGACTCCAGCCGGATCAGCGGCGAGGGCTCTCCGATGACCATGTCACCATGGAACCAGACCACAGCTTCTTCTCCCTTCACTAAGTCACCATTCACGCCCTTCGAAGAGAATCTCCCCAAGAACGGCCTCATCGGCTCTCTCATCCGCGAGGAAGGTCACATATACTCGTTGGCCGCCACAAGGGACCTTTTGTACACCGGCTCCGACAGCAAGAACATCAGAGTCTGGAAGAATCTCAAGGAGTATACCGGTTTTAAATCCAATAGCGGTTTGGTTAAGGCCATCGTTATCTCCGGTGAGAAGCTCTTCACTGGTCACCAGGATGGTAAGGTTAGGGTTTGGAGAGTCTCTACCAAAGACCCTAGAGTTCATAAACGCGCCGGGACGTTGCCGACTCTGATGGATATTTTCAAATACTCCGTCAAACCTAAAAACTACGTTCAAGTAAGGAAGCAGAAGACGCTTTGGATCAGGCACTCCGACGCCGTTTCATGTCTGAGCTTGAGCGAGGACAAGATGCTTTTGTACTCAGCCTCATGGGACCGCACGGTCAAAGTGTGGCGTGTAGAGACCTCTAAATGCATTGAGTCCATCTACGCCCACGACGACGCCGTGAACTCCGTCGTGGCGAGCGTGGATGGGCTGTTGTACACCGGCGCGGCGGACGGGACGGTGAAGGTTTGGAAGAAGGAGCAGAGTGGGAAGATTATAAAGCACTCTTTGGTGCAGACGCTGCTGAAGCAAGAGAGCGCGGTGACTGCACTCGCGGTGAGCGCGTCGGGGGCAATCGTGTATTGCGGGTCGTCGGATGGGTTGGTTAATTTCTGGGAGCGGGAGAAGCAGTTGTCGCATGGTGGGGTCCTCAGGGGGCACAGGCTGGCTGTGCTGTGCTTGGCGGCGGCTGGGAACTTGGTGTGCAGCGGCTCGGCTGATAAGACCATATGCGTGTGGAGAAGGGAGGGCGTGATCCACACGTGCCTGTCGGTTTTGGCCGGCCATACTGGGCCGGTGAAGTGCTTGGCGGTTGAGGAGGATCGTGAGCCGTCGTTGAGGGGTGATCGACGGTGGATAGTGTACAGTGGGAGCCTAGATAAGTCTGTGAAGGTGTGGGGGGTGTCGGAGCAAGCGCCGGAGCTCACTCCCATGTCTATGCATTCACATACACCCAAGTTGATTACCGACGCCGACTCGTTACCGTCGGATGGGAGCTACTCTTCGTCGAGCCGGCCTGGCAAGGACAGAAGGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009846 GO:0009856 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0022414 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032501 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044706 GO:0045159 GO:0046777 GO:0051301 GO:0051704 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

468

Amino Acids

51.36

Weight (kDa)

9.25

Isoelectric Point (pI)

42.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_SCAP	PF24017	86 - 333	4.1e-10	SCAP Beta-propeller
Beta-prop_Nup120_160	PF11715	98 - 249	7.7e-06	Nucleoporin Nup120/160, beta-propeller domain
WDR55	PF24796	185 - 289	3.7e-10	WDR55
WD40_Gbeta	PF25391	200 - 368	9.7e-15	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	202 - 292	2.2e-09	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	205 - 282	3e-08	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	206 - 393	4.1e-22	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	207 - 283	1.7e-14	WDR5 beta-propeller domain
WD40	PF00400	208 - 238	4.7e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	209 - 260	1.8e-06	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	209 - 350	4.7e-13	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	210 - 396	5.8e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	210 - 330	1.8e-16	WDR3 second beta-propeller domain
EIF3I	PF24805	210 - 331	5.2e-06	EIF3I
Beta-prop_WDR3_1st	PF25173	210 - 280	4.3e-12	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	210 - 395	7.5e-12	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	212 - 331	3.2e-15	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	216 - 370	1e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	225 - 331	5.6e-07	WDR90/POC16, second beta-propeller
WD40	PF00400	244 - 279	5.9e-06	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	267 - 425	3.9e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	289 - 427	6.5e-19	WDR3 first beta-propeller domain
WDR55	PF24796	297 - 424	2.4e-06	WDR55
Beta-prop_TEP1_2nd	PF25047	311 - 432	6.9e-12	TEP-1 second beta-propeller
WD40_Prp19	PF24814	347 - 455	1.5e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	350 - 428	2.8e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26490
fragaria_vesca	FvH4_2g40541
malus_domestica	MD00G1038200.v1.1 MD08G1039100.v1.1
prunus_persica	Prupe.1G386600_v2.0.a1
pyrus_communis	pycom08g03080
rosa_chinensis	RchiOBHm_Chr6g0300181
rosa_laevigata	RLG00000011360
rosa_multiflora	Rmu_sc0001670.1_g000038
rosa_roxburghii	Rroxscaffold_7G00168030
rosa_samantha	Rh6AG406000 Rh6BG414000 Rh6CG421200 Rh6DG406800
rosa_wichuraiana	Rw6G035540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	863
AasI	GACNNNNNNGTC	1 cut(s)	701
AatII	GACGTC	1 cut(s)	111
AccBSI	CCGCTC	1 cut(s)	985
AccI	GTMKAC	1 cut(s)	398
AccII	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
AclWI	GGATC	5 cut(s)	68, 173, 629, 1114, 1194
AcoI	YGGCCR	4 cut(s)	87, 324, 1143, 1147
AcuI	CTGAAG	1 cut(s)	911
AcvI	CACGTG	1 cut(s)	1128
AcyI	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
AfaI	GTAC	4 cut(s)	347, 680, 803, 1229
AfiI	CCNNNNNNNGG	3 cut(s)	275, 333, 1355
AflIII	ACRYGT	1 cut(s)	1125
AgeI	ACCGGT	1 cut(s)	401
AgsI	TTSAA	2 cut(s)	565, 596
AjnI	CCWGG	2 cut(s)	471, 1381
AluBI	AGCT	6 cut(s)	33, 218, 457, 654, 1288, 1362
AluI	AGCT	6 cut(s)	33, 218, 457, 654, 1288, 1362
Alw21I	GWGCWC	1 cut(s)	1290
Alw26I	GTCTC	2 cut(s)	505, 716
AlwI	GGATC	5 cut(s)	68, 173, 629, 1114, 1194
AlwNI	CAGNNNCTG	2 cut(s)	378, 886
AoxI	GGCC	8 cut(s)	87, 277, 324, 432, 1143, 1147, 1157, 1379
ApeKI	GCWGC	2 cut(s)	886, 1071
ArsI	GACNNNNNNTTYG	2 cut(s)	558, 590
AsiGI	ACCGGT	1 cut(s)	401
Asp700I	GAANNNNTTC	1 cut(s)	458
AspA2I	CCTAGG	1 cut(s)	38
AspLEI	GCGC	6 cut(s)	143, 533, 812, 905, 929, 1282
AspS9I	GGNCC	4 cut(s)	337, 694, 1012, 1157
AsuC2I	CCSGG	2 cut(s)	104, 535
AsuHPI	GGTGA	9 cut(s)	189, 231, 461, 461, 838, 919, 934, 1174, 1220
AsuII	TTCGAA	1 cut(s)	255
AvaII	GGWCC	3 cut(s)	337, 694, 1012
AvrII	CCTAGG	1 cut(s)	38
AxyI	CCTNAGG	1 cut(s)	1016
BaeGI	GKGCMC	1 cut(s)	1026
BanII	GRGCYC	2 cut(s)	182, 1290
BbrPI	CACGTG	1 cut(s)	1128
BbsI	GAAGAC	1 cut(s)	617
Bbv12I	GWGCWC	1 cut(s)	1290
BbvI	GCAGC	2 cut(s)	873, 1083
BccI	CCATC	7 cut(s)	443, 470, 547, 752, 785, 956, 1349
BceAI	ACGGC	5 cut(s)	76, 292, 623, 749, 1180
BciT130I	CCWGG	2 cut(s)	473, 1383
BcnI	CCSGG	2 cut(s)	104, 535
BcoDI	GTCTC	2 cut(s)	505, 716
BfaI	CTAG	3 cut(s)	39, 516, 1241
BfoI	RGCGCY	1 cut(s)	1283
BglI	GCCNNNNNGGC	2 cut(s)	285, 1383
BisI	GCNGC	8 cut(s)	172, 327, 813, 887, 1050, 1053, 1072, 1075
BlnI	CCTAGG	1 cut(s)	38
BlsI	GCNGC	8 cut(s)	173, 328, 814, 888, 1051, 1054, 1073, 1076
Bme1390I	CCNGG	4 cut(s)	104, 473, 535, 1383
Bme18I	GGWCC	3 cut(s)	337, 694, 1012
BmgT120I	GGNCC	4 cut(s)	337, 694, 1012, 1157
BmiI	GGNNCC	6 cut(s)	206, 338, 355, 695, 1013, 1238
BmrFI	CCNGG	4 cut(s)	104, 473, 535, 1383
BmrI	ACTGGG	1 cut(s)	1164
BmsI	GCATC	1 cut(s)	660
BmuI	ACTGGG	1 cut(s)	1164
BpiI	GAAGAC	1 cut(s)	617
BpmI	CTGGAG	1 cut(s)	142
Bpu14I	TTCGAA	1 cut(s)	255
BpuEI	CTTGAG	2 cut(s)	374, 676
BpuMI	CCSGG	2 cut(s)	104, 535
BsaAI	YACGTR	1 cut(s)	1128
BsaHI	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
BsaI	GGTCTC	1 cut(s)	716
BsaJI	CCNNGG	3 cut(s)	38, 102, 200
BsaWI	WCCGGW	2 cut(s)	401, 446
BsaXI	ACNNNNNCTCC	4 cut(s)	338, 368, 980, 1010
Bsc4I	CCNNNNNNNGG	3 cut(s)	275, 333, 1355
Bse118I	RCCGGY	6 cut(s)	350, 401, 806, 1145, 1159, 1377
Bse1I	ACTGG	2 cut(s)	469, 1159
Bse21I	CCTNAGG	1 cut(s)	1016
BseBI	CCWGG	2 cut(s)	473, 1383
BseDI	CCNNGG	3 cut(s)	38, 102, 200
BseGI	GGATG	6 cut(s)	18, 294, 481, 796, 967, 1360
BseLI	CCNNNNNNNGG	3 cut(s)	275, 333, 1355
BseMII	CTCAG	3 cut(s)	641, 696, 1030
BseNI	ACTGG	2 cut(s)	469, 1159
BseRI	GAGGAG	1 cut(s)	1196
BseSI	GKGCMC	1 cut(s)	1026
BseXI	GCAGC	2 cut(s)	873, 1083
BseYI	CCCAGC	1 cut(s)	1055
BsgI	GTGCAG	3 cut(s)	897, 899, 1090
Bsh1236I	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
BshFI	GGCC	8 cut(s)	89, 279, 326, 434, 1145, 1149, 1159, 1381
BshTI	ACCGGT	1 cut(s)	401
BsiHKAI	GWGCWC	1 cut(s)	1290
BslFI	GGGAC	5 cut(s)	85, 350, 550, 707, 835
BslI	CCNNNNNNNGG	3 cut(s)	275, 333, 1355
BsmAI	GTCTC	2 cut(s)	505, 716
BsmFI	GGGAC	5 cut(s)	85, 350, 550, 707, 835
BsmI	GAATGC	1 cut(s)	1306
BsnI	GGCC	8 cut(s)	89, 279, 326, 434, 1145, 1149, 1159, 1381
Bso31I	GGTCTC	1 cut(s)	716
Bsp119I	TTCGAA	1 cut(s)	255
Bsp1286I	GDGCHC	3 cut(s)	182, 1026, 1290
Bsp1407I	TGTACA	3 cut(s)	345, 801, 1227
Bsp143I	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
Bsp19I	CCATGG	1 cut(s)	200
BspANI	GGCC	8 cut(s)	89, 279, 326, 434, 1145, 1149, 1159, 1381
BspCNI	CTCAG	3 cut(s)	642, 695, 1029
BspFNI	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
BspLI	GGNNCC	6 cut(s)	206, 338, 355, 695, 1013, 1238
BspPI	GGATC	5 cut(s)	68, 173, 629, 1114, 1194
BspQI	GCTCTTC	1 cut(s)	464
BspT104I	TTCGAA	1 cut(s)	255
BspTNI	GGTCTC	1 cut(s)	716
BsrBI	CCGCTC	1 cut(s)	985
BsrFI	RCCGGY	6 cut(s)	350, 401, 806, 1145, 1159, 1377
BsrGI	TGTACA	3 cut(s)	345, 801, 1227
BsrI	ACTGG	2 cut(s)	469, 1159
BssAI	RCCGGY	6 cut(s)	350, 401, 806, 1145, 1159, 1377
BssECI	CCNNGG	3 cut(s)	38, 102, 200
BssMI	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
BssNAI	GTATAC	1 cut(s)	399
BssNI	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
BssT1I	CCWWGG	2 cut(s)	38, 200
Bst1107I	GTATAC	1 cut(s)	399
Bst2UI	CCWGG	2 cut(s)	473, 1383
Bst4CI	ACNGT	6 cut(s)	137, 703, 826, 1218, 1232, 1350
Bst6I	CTCTTC	3 cut(s)	252, 464, 1372
BstACI	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
BstAPI	GCANNNNNTGC	1 cut(s)	886
BstAUI	TGTACA	3 cut(s)	345, 801, 1227
BstBAI	YACGTR	1 cut(s)	1128
BstBI	TTCGAA	1 cut(s)	255
BstC8I	GCNNGC	5 cut(s)	784, 1032, 1147, 1278, 1379
BstDEI	CTNAG	4 cut(s)	234, 650, 682, 1016
BstDSI	CCRYGG	1 cut(s)	200
BstEII	GGTNACC	1 cut(s)	467
BstF5I	GGATG	6 cut(s)	18, 294, 481, 796, 967, 1360
BstFNI	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
BstH2I	RGCGCY	1 cut(s)	1283
BstHHI	GCGC	6 cut(s)	143, 533, 812, 905, 929, 1282
BstKTI	GATC	6 cut(s)	76, 168, 624, 1122, 1189, 1213
BstMAI	GTCTC	2 cut(s)	505, 716
BstMBI	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
BstNI	CCWGG	2 cut(s)	473, 1383
BstPI	GGTNACC	1 cut(s)	467
BstSCI	CCNGG	4 cut(s)	102, 471, 533, 1381
BstSLI	GKGCMC	1 cut(s)	1026
BstUI	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
BstV1I	GCAGC	2 cut(s)	873, 1083
BstV2I	GAAGAC	1 cut(s)	617
BstZ17I	GTATAC	1 cut(s)	399
Bsu36I	CCTNAGG	1 cut(s)	1016
BsuRI	GGCC	8 cut(s)	89, 279, 326, 434, 1145, 1149, 1159, 1381
BtgI	CCRYGG	1 cut(s)	200
BtsCI	GGATG	6 cut(s)	18, 294, 481, 796, 967, 1360
BtsIMutI	CAGTG	3 cut(s)	462, 1237, 1399
Cac8I	GCNNGC	5 cut(s)	784, 1032, 1147, 1278, 1379
CaiI	CAGNNNCTG	2 cut(s)	378, 886
CfoI	GCGC	6 cut(s)	143, 533, 812, 905, 929, 1282
Cfr10I	RCCGGY	6 cut(s)	350, 401, 806, 1145, 1159, 1377
Cfr13I	GGNCC	4 cut(s)	337, 694, 1012, 1157
CseI	GACGC	8 cut(s)	52, 132, 622, 644, 770, 892, 918, 1343
Csp6I	GTAC	4 cut(s)	346, 679, 802, 1228
CspAI	ACCGGT	1 cut(s)	401
CviAII	CATG	6 cut(s)	193, 201, 645, 690, 1004, 1297
CviQI	GTAC	4 cut(s)	346, 679, 802, 1228
DdeI	CTNAG	4 cut(s)	234, 650, 682, 1016
DpnI	GATC	6 cut(s)	75, 167, 623, 1121, 1188, 1212
DpnII	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
DraI	TTTAAA	1 cut(s)	409
DrdI	GACNNNNNNGTC	1 cut(s)	701
DseDI	GACNNNNNNGTC	1 cut(s)	701
EaeI	YGGCCR	4 cut(s)	87, 324, 1143, 1147
Eam1104I	CTCTTC	3 cut(s)	252, 464, 1372
EarI	CTCTTC	3 cut(s)	252, 464, 1372
EciI	GGCGGA	1 cut(s)	830
Ecl136II	GAGCTC	1 cut(s)	1288
Eco130I	CCWWGG	2 cut(s)	38, 200
Eco24I	GRGCYC	2 cut(s)	182, 1290
Eco31I	GGTCTC	1 cut(s)	716
Eco47I	GGWCC	3 cut(s)	337, 694, 1012
Eco53kI	GAGCTC	1 cut(s)	1288
Eco57I	CTGAAG	1 cut(s)	911
Eco72I	CACGTG	1 cut(s)	1128
Eco81I	CCTNAGG	1 cut(s)	1016
Eco91I	GGTNACC	1 cut(s)	467
EcoICRI	GAGCTC	1 cut(s)	1288
EcoO109I	RGGNCCY	2 cut(s)	337, 1012
EcoO65I	GGTNACC	1 cut(s)	467
EcoRII	CCWGG	2 cut(s)	471, 1381
EcoT14I	CCWWGG	2 cut(s)	38, 200
EcoT22I	ATGCAT	2 cut(s)	737, 1308
EcoT38I	GRGCYC	2 cut(s)	182, 1290
ErhI	CCWWGG	2 cut(s)	38, 200
FaeI	CATG	6 cut(s)	196, 204, 648, 693, 1007, 1300
FaqI	GGGAC	5 cut(s)	85, 350, 550, 707, 835
FatI	CATG	6 cut(s)	192, 200, 644, 689, 1003, 1296
FauI	CCCGC	2 cut(s)	944, 978
FauNDI	CATATG	1 cut(s)	1094
FblI	GTMKAC	1 cut(s)	398
Fnu4HI	GCNGC	8 cut(s)	172, 327, 813, 887, 1050, 1053, 1072, 1075
FokI	GGATG	6 cut(s)	25, 281, 488, 803, 974, 1367
FriOI	GRGCYC	2 cut(s)	182, 1290
FseI	GGCCGGCC	1 cut(s)	1149
Fsp4HI	GCNGC	8 cut(s)	172, 327, 813, 887, 1050, 1053, 1072, 1075
FspBI	CTAG	3 cut(s)	39, 516, 1241
GlaI	GCGC	6 cut(s)	142, 532, 811, 904, 928, 1281
GluI	GCNGC	8 cut(s)	172, 327, 813, 887, 1050, 1053, 1072, 1075
GsaI	CCCAGC	1 cut(s)	1059
GsuI	CTGGAG	1 cut(s)	142
HaeII	RGCGCY	1 cut(s)	1283
HaeIII	GGCC	8 cut(s)	89, 279, 326, 434, 1145, 1149, 1159, 1381
HgaI	GACGC	8 cut(s)	52, 132, 622, 644, 770, 892, 918, 1343
HhaI	GCGC	6 cut(s)	143, 533, 812, 905, 929, 1282
Hin1I	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
Hin1II	CATG	6 cut(s)	196, 204, 648, 693, 1007, 1300
Hin6I	GCGC	6 cut(s)	141, 531, 810, 903, 927, 1280
HinP1I	GCGC	6 cut(s)	141, 531, 810, 903, 927, 1280
HinfI	GANTC	8 cut(s)	155, 262, 375, 385, 498, 547, 740, 1340
HphI	GGTGA	9 cut(s)	189, 231, 461, 461, 838, 919, 934, 1174, 1220
Hpy166II	GTNNAC	5 cut(s)	348, 399, 769, 804, 1228
Hpy188III	TCNNGA	3 cut(s)	116, 379, 1190
Hpy8I	GTNNAC	5 cut(s)	348, 399, 769, 804, 1228
HpyAV	CCTTC	9 cut(s)	122, 238, 262, 299, 823, 835, 1101, 1249, 1389
HpyCH4III	ACNGT	6 cut(s)	137, 703, 826, 1218, 1232, 1350
HpyCH4IV	ACGT	4 cut(s)	108, 539, 591, 1127
HpyCH4V	TGCA	5 cut(s)	735, 880, 914, 1071, 1306
HpyF3I	CTNAG	4 cut(s)	234, 650, 682, 1016
HpySE526I	ACGT	4 cut(s)	108, 539, 591, 1127
Hsp92I	GRCGYC	5 cut(s)	63, 108, 636, 762, 1335
Hsp92II	CATG	6 cut(s)	196, 204, 648, 693, 1007, 1300
HspAI	GCGC	6 cut(s)	141, 531, 810, 903, 927, 1280
KroI	GCCGGC	2 cut(s)	1145, 1377
KroNI	GCCGGC	2 cut(s)	1147, 1379
Kzo9I	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
LguI	GCTCTTC	1 cut(s)	464
LmnI	GCTCC	7 cut(s)	359, 844, 982, 1236, 1273, 1285, 1359
Lsp1109I	GCAGC	2 cut(s)	873, 1083
LweI	GCATC	1 cut(s)	660
MaeI	CTAG	3 cut(s)	39, 516, 1241
MaeII	ACGT	4 cut(s)	108, 539, 591, 1127
MaeIII	GTNAC	6 cut(s)	195, 237, 308, 467, 907, 1344
MalI	GATC	6 cut(s)	75, 167, 623, 1121, 1188, 1212
MbiI	CCGCTC	1 cut(s)	985
MboI	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
MboII	GAAGA	8 cut(s)	213, 269, 394, 451, 622, 850, 868, 1359
MhlI	GDGCHC	3 cut(s)	182, 1026, 1290
MluCI	AATT	2 cut(s)	49, 973
MlyI	GAGTC	6 cut(s)	149, 384, 507, 541, 749, 1334
MmeI	TCCRAC	6 cut(s)	45, 381, 657, 939, 1251, 1332
Mph1103I	ATGCAT	2 cut(s)	737, 1308
MroNI	GCCGGC	2 cut(s)	1145, 1377
MroXI	GAANNNNTTC	1 cut(s)	458
MseI	TTAA	3 cut(s)	408, 429, 972
MslI	CAYNNNNRTG	4 cut(s)	474, 1097, 1295, 1301
MspA1I	CMGCKG	2 cut(s)	171, 1074
MspR9I	CCNGG	4 cut(s)	104, 473, 535, 1383
Mva1269I	GAATGC	1 cut(s)	1306
MvaI	CCWGG	2 cut(s)	473, 1383
MvnI	CGCG	7 cut(s)	143, 300, 531, 812, 905, 920, 929
NaeI	GCCGGC	2 cut(s)	1147, 1379
NciI	CCSGG	2 cut(s)	104, 535
NcoI	CCATGG	1 cut(s)	200
NdeI	CATATG	1 cut(s)	1094
NdeII	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
NgoMIV	GCCGGC	2 cut(s)	1145, 1377
NlaIII	CATG	6 cut(s)	196, 204, 648, 693, 1007, 1300
NlaIV	GGNNCC	6 cut(s)	206, 338, 355, 695, 1013, 1238
NmeAIII	GCCGAG	1 cut(s)	1058
NmuCI	GTSAC	5 cut(s)	195, 237, 308, 467, 907
NsiI	ATGCAT	2 cut(s)	737, 1308
NspV	TTCGAA	1 cut(s)	255
PciSI	GCTCTTC	1 cut(s)	464
PcsI	WCGNNNNNNNCGW	5 cut(s)	60, 114, 756, 762, 783
PctI	GAATGC	1 cut(s)	1306
PdiI	GCCGGC	2 cut(s)	1147, 1379
PdmI	GAANNNNTTC	1 cut(s)	458
PfeI	GAWTC	2 cut(s)	262, 385
PflFI	GACNNNGTC	1 cut(s)	193
PinAI	ACCGGT	1 cut(s)	401
PkrI	GCNGC	8 cut(s)	173, 328, 814, 888, 1051, 1054, 1073, 1076
PleI	GAGTC	6 cut(s)	149, 383, 506, 541, 748, 1334
PmaCI	CACGTG	1 cut(s)	1128
PmlI	CACGTG	1 cut(s)	1128
PpsI	GAGTC	6 cut(s)	149, 383, 506, 541, 748, 1334
Ppu21I	YACGTR	1 cut(s)	1128
PpuMI	RGGWCCY	2 cut(s)	337, 1012
PsiI	TTATAA	1 cut(s)	863
Psp124BI	GAGCTC	1 cut(s)	1290
Psp5II	RGGWCCY	2 cut(s)	337, 1012
Psp6I	CCWGG	2 cut(s)	471, 1381
PspCI	CACGTG	1 cut(s)	1128
PspEI	GGTNACC	1 cut(s)	467
PspFI	CCCAGC	1 cut(s)	1055
PspGI	CCWGG	2 cut(s)	471, 1381
PspN4I	GGNNCC	6 cut(s)	206, 338, 355, 695, 1013, 1238
PspPI	GGNCC	4 cut(s)	337, 694, 1012, 1157
PspPPI	RGGWCCY	2 cut(s)	337, 1012
PstNI	CAGNNNCTG	2 cut(s)	378, 886
PsyI	GACNNNGTC	1 cut(s)	193
RigI	GGCCGGCC	1 cut(s)	1149
RsaI	GTAC	4 cut(s)	347, 680, 803, 1229
RsaNI	GTAC	4 cut(s)	346, 679, 802, 1228
RseI	CAYNNNNRTG	4 cut(s)	474, 1097, 1295, 1301
SacI	GAGCTC	1 cut(s)	1290
SapI	GCTCTTC	1 cut(s)	464
SaqAI	TTAA	3 cut(s)	408, 429, 972
SatI	GCNGC	8 cut(s)	172, 327, 813, 887, 1050, 1053, 1072, 1075
Sau3AI	GATC	6 cut(s)	73, 165, 621, 1119, 1186, 1210
Sau96I	GGNCC	4 cut(s)	337, 694, 1012, 1157
SchI	GAGTC	6 cut(s)	149, 384, 507, 541, 749, 1334
ScrFI	CCNGG	4 cut(s)	104, 473, 535, 1383
SduI	GDGCHC	3 cut(s)	182, 1026, 1290
SfaNI	GCATC	1 cut(s)	660
SfuI	TTCGAA	1 cut(s)	255
SgrAI	CRCCGGYG	1 cut(s)	806
SinI	GGWCC	3 cut(s)	337, 694, 1012
SmiMI	CAYNNNNRTG	4 cut(s)	474, 1097, 1295, 1301
SmlI	CTYRAG	2 cut(s)	389, 655
SmoI	CTYRAG	2 cut(s)	389, 655
Sse9I	AATT	2 cut(s)	49, 973
SspMI	CTAG	3 cut(s)	39, 516, 1241
SstI	GAGCTC	1 cut(s)	1290
StyD4I	CCNGG	4 cut(s)	102, 471, 533, 1381
StyI	CCWWGG	2 cut(s)	38, 200
TaaI	ACNGT	6 cut(s)	137, 703, 826, 1218, 1232, 1350
TaiI	ACGT	4 cut(s)	111, 542, 594, 1130
TaqI	TCGA	4 cut(s)	153, 255, 1213, 1373
TasI	AATT	2 cut(s)	49, 973
TatI	WGTACW	4 cut(s)	345, 678, 801, 1227
TauI	GCSGC	6 cut(s)	174, 329, 815, 1052, 1055, 1077
TfiI	GAWTC	2 cut(s)	262, 385
Tru1I	TTAA	3 cut(s)	408, 429, 972
Tru9I	TTAA	3 cut(s)	408, 429, 972
TscAI	CASTG	2 cut(s)	469, 1237
TseFI	GTSAC	5 cut(s)	195, 237, 308, 467, 907
TseI	GCWGC	2 cut(s)	886, 1071
Tsp45I	GTSAC	5 cut(s)	195, 237, 308, 467, 907
TspDTI	ATGAA	2 cut(s)	512, 633
TspGWI	ACGGA	2 cut(s)	562, 763
TspRI	CASTG	2 cut(s)	469, 1237
Tth111I	GACNNNGTC	1 cut(s)	193
VpaK11BI	GGWCC	3 cut(s)	337, 694, 1012
XcmI	CCANNNNNNNNNTGG	1 cut(s)	421
XmaJI	CCTAGG	1 cut(s)	38
XmiI	GTMKAC	1 cut(s)	398
XmnI	GAANNNNTTC	1 cut(s)	458
XspI	CTAG	3 cut(s)	39, 516, 1241
ZraI	GACGTC	1 cut(s)	109
Zsp2I	ATGCAT	2 cut(s)	737, 1308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.