Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0016067

Physical Location & Seq

Genomic Coordinates Forward (+)

8860056 .. 8861450

Gene Structure 1395 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011360

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGAGAGACAAGATGTTTGCCGATGACAGCAGCTTACCTAGGGCCAGTACTTTCGGCAATGCCGTCCATTCCGATCCCAATATTTCGGCCTCCGCGATGTCCCCCGACGTCATTCACGACTACCAAATGCGAAACAGTAGCGCCGCGGCCTTCGACCCGACCCGGCTCAGCGGCGAGGGCTCTCCCATGACTATGTCACCATGGAACCAGAACTCTGCCTCTCCGTTCACCAAGTCGCCGTGGACGCCGTTTGAAGACAAGGTCCCCAAGAACGGCCTCCTCGGCTCTCTCATTCGCGAGGAAGGCCACATCTACTCTCTCGCCGCCACCGGAGACCTTCTGTACACCGGTTCCGACAGCAAGAATATCAGAGTATGGAAGAATCTCAAGGAGTACAGCGGCTTCAAGTCTAACAGCGGCTTGGTGAAAGCAATTGTTATCTCCGGGGAGAAGCTCTTCACAGGTCACCAGGACGGCAAGATTAGGGTTTGGAGAGTCTCTTCCAAAGACCCGAAAGTTCACAAACGCGCCGGGACGATGCCGACTCTGATGGACATTTTCAAGTACTCCGTCAAACCCAGCAACTATGTTCAAGTTAGGAAAACGAAAGCGCTTTGGATCAGGCACTCCGACGCCGTTTCCTGTCTGAGCTTGAGCGAAGACAAGAGGCTTTTGTATTCTGCCTCCTGGGACCGCACGCTAAAAGTATGGCGTATCGACACCTCCAAATGCATCGAGTCCATCCACGCGCACGACGACGCCGTTAACTCCGTCGTGGCCAGCGTGGAAGGGCTGTTGTACACCGGCGCGGCGGACGGGACGGTGAAGGTTTGGAAGAAGGAGCAGAGTGGGAAGATGATAAAACACTCTTTGGTGCAGACGCTGCTGAAGCAAGAGAGTGCGGTGACCGCACTCGCGGTGAGCGCGTCTGGGTCTCTCGTCTATTGTGGGTCGTCGGATGGGTTGGTTAATTTCTGGGAGCGCGAGAAGCAGTTATCGCATGGTGGGGTCCTCAAGGGCCACAGGCTGGCTGTGCTGTGCTTGGCGGCGGCTGGGAGCCTGGTGTTCAGCGGCTCAGCTGATAAGACCATATGCGTGTGGAGGAGGGACGGCGTGATCCACACGTGCCTGTCGGTTTTGGCGGGCCACTCTGGGCCGGTGAAGTGCTTGGCCGTTGAGGAGGATCGTGAACGTTCGACCAACGGCGATCACCGGTGGATCGTGTACAGTGGGAGCCTGGATAAGTCTGTGAAGGTGTGGGGGGTGTCGGAGCAAGCTCCGGAGCTCACTCCCATGTCCATGCAGACCCAGTTGAATACCGACGCCGACTCGCTACCGTCCGAGGGAAGCTACTCTTCTTCGAGCCGCCGGTCCGGCCAGGAGAGAAGGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009846 GO:0009856 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0022414 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032501 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044706 GO:0045159 GO:0046777 GO:0051301 GO:0051704 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

465

Amino Acids

50.81

Weight (kDa)

9.11

Isoelectric Point (pI)

41.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_SCAP	PF24017	84 - 332	7.9e-12	SCAP Beta-propeller
Beta-prop_Nup120_160	PF11715	99 - 250	5.3e-06	Nucleoporin Nup120/160, beta-propeller domain
Beta-prop_WDR5	PF25175	109 - 282	1.5e-15	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	148 - 290	1.2e-13	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	148 - 328	1.6e-06	MABP1/WDR62 second WD40 domain
WDR55	PF24796	178 - 286	2e-10	WDR55
WD40_Gbeta	PF25391	202 - 367	6e-14	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	202 - 290	1e-07	TEP-1 second beta-propeller
WD40	PF00400	208 - 237	5.1e-07	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	208 - 349	1.9e-13	CDC20/Fizzy WD40 domain
EIF3I	PF24805	209 - 330	5.1e-06	EIF3I
Beta-prop_EML	PF23409	209 - 395	5.2e-12	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	209 - 279	2.3e-12	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	209 - 329	6.9e-17	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	209 - 397	9.5e-07	MABP1/WDR62 second WD40 domain
Beta-prop_THOC3	PF25174	209 - 330	8.4e-15	THOC3 beta-propeller domain
WD40_Prp19	PF24814	211 - 330	2.2e-15	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	215 - 368	5.6e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	220 - 330	9.7e-08	WDR90/POC16, second beta-propeller
Beta-prop_WDR5	PF25175	266 - 424	1.6e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	288 - 426	5.6e-20	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	296 - 429	2e-09	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	311 - 430	3.8e-11	TEP-1 second beta-propeller
WD40_Prp19	PF24814	346 - 428	2.8e-07	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	350 - 427	4e-07	THOC3 beta-propeller domain
WD40	PF00400	375 - 420	4.4e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26490
fragaria_vesca	FvH4_2g40541
malus_domestica	MD00G1038200.v1.1 MD08G1039100.v1.1
prunus_persica	Prupe.1G386600_v2.0.a1
pyrus_communis	pycom08g03080
rosa_chinensis	RchiOBHm_Chr6g0300181
rosa_laevigata	RLG00000011360
rosa_multiflora	Rmu_sc0001670.1_g000038
rosa_roxburghii	Rroxscaffold_7G00168030
rosa_samantha	Rh6AG406000 Rh6BG414000 Rh6CG421200 Rh6DG406800
rosa_wichuraiana	Rw6G035540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	111
AccB7I	CCANNNNNTGG	1 cut(s)	1027
AccII	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
AccIII	TCCGGA	1 cut(s)	1279
AclI	AACGTT	1 cut(s)	1192
AclWI	GGATC	5 cut(s)	68, 626, 1111, 1191, 1226
AcoI	YGGCCR	3 cut(s)	777, 1170, 1375
AcuI	CTGAAG	1 cut(s)	908
AcvI	CACGTG	1 cut(s)	1125
AcyI	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
AfaI	GTAC	6 cut(s)	49, 344, 395, 566, 800, 1226
AfeI	AGCGCT	1 cut(s)	612
AfiI	CCNNNNNNNGG	3 cut(s)	272, 1027, 1343
AflIII	ACRYGT	1 cut(s)	1122
AgeI	ACCGGT	2 cut(s)	347, 1212
AgsI	TTSAA	5 cut(s)	254, 406, 562, 593, 1315
AjnI	CCWGG	5 cut(s)	468, 686, 1059, 1236, 1377
AluBI	AGCT	7 cut(s)	33, 454, 651, 1079, 1277, 1285, 1350
AluI	AGCT	7 cut(s)	33, 454, 651, 1079, 1277, 1285, 1350
Alw21I	GWGCWC	1 cut(s)	1287
Alw26I	GTCTC	3 cut(s)	327, 502, 939
AlwI	GGATC	5 cut(s)	68, 626, 1111, 1191, 1226
AlwNI	CAGNNNCTG	1 cut(s)	883
Aor13HI	TCCGGA	1 cut(s)	1279
Aor51HI	AGCGCT	1 cut(s)	612
ApeKI	GCWGC	2 cut(s)	30, 883
ArsI	GACNNNNNNTTYG	1 cut(s)	31
AsiGI	ACCGGT	2 cut(s)	347, 1212
Asp700I	GAANNNNTTC	1 cut(s)	455
AspA2I	CCTAGG	1 cut(s)	38
AspLEI	GCGC	7 cut(s)	143, 530, 613, 751, 809, 926, 984
AspS9I	GGNCC	8 cut(s)	42, 262, 691, 1009, 1018, 1144, 1154, 1371
AsuC2I	CCSGG	3 cut(s)	163, 445, 532
AsuHPI	GGTGA	9 cut(s)	189, 220, 436, 458, 835, 916, 931, 1171, 1202
AvaII	GGWCC	4 cut(s)	262, 691, 1009, 1371
AvrII	CCTAGG	1 cut(s)	38
BalI	TGGCCA	1 cut(s)	779
BanII	GRGCYC	2 cut(s)	182, 1287
BarI	GAAGNNNNNNTAC	2 cut(s)	386, 418
BbrPI	CACGTG	1 cut(s)	1125
BbsI	GAAGAC	2 cut(s)	261, 666
Bbv12I	GWGCWC	1 cut(s)	1287
BbvI	GCAGC	2 cut(s)	42, 870
BccI	CCATC	3 cut(s)	544, 749, 953
BciT130I	CCWGG	5 cut(s)	470, 688, 1061, 1238, 1379
BcnI	CCSGG	3 cut(s)	163, 445, 532
BcoDI	GTCTC	3 cut(s)	327, 502, 939
BfaI	CTAG	1 cut(s)	39
BfoI	RGCGCY	2 cut(s)	144, 614
BglI	GCCNNNNNGGC	2 cut(s)	282, 1374
BlnI	CCTAGG	1 cut(s)	38
BlpI	GCTNAGC	2 cut(s)	167, 1075
BmcAI	AGTACT	2 cut(s)	49, 566
Bme1390I	CCNGG	8 cut(s)	163, 445, 470, 532, 688, 1061, 1238, 1379
Bme18I	GGWCC	4 cut(s)	262, 691, 1009, 1371
BmgT120I	GGNCC	8 cut(s)	42, 262, 691, 1009, 1018, 1144, 1154, 1371
BmiI	GGNNCC	7 cut(s)	206, 264, 352, 692, 1010, 1058, 1235
BmrFI	CCNGG	8 cut(s)	163, 445, 470, 532, 688, 1061, 1238, 1379
BmrI	ACTGGG	1 cut(s)	1303
BmsI	GCATC	2 cut(s)	528, 741
BmuI	ACTGGG	1 cut(s)	1303
BpiI	GAAGAC	2 cut(s)	261, 666
Bpu1102I	GCTNAGC	2 cut(s)	167, 1075
BpuEI	CTTGAG	3 cut(s)	371, 673, 998
BpuMI	CCSGG	3 cut(s)	163, 445, 532
BsaAI	YACGTR	1 cut(s)	1125
BsaHI	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
BsaI	GGTCTC	2 cut(s)	327, 939
BsaJI	CCNNGG	8 cut(s)	38, 144, 200, 239, 280, 444, 687, 1341
BsaWI	WCCGGW	4 cut(s)	329, 347, 1212, 1279
Bsc4I	CCNNNNNNNGG	3 cut(s)	272, 1027, 1343
Bse118I	RCCGGY	5 cut(s)	347, 803, 1156, 1212, 1368
Bse1I	ACTGG	2 cut(s)	45, 1309
Bse3DI	GCAATG	1 cut(s)	64
BseAI	TCCGGA	1 cut(s)	1279
BseBI	CCWGG	5 cut(s)	470, 688, 1061, 1238, 1379
BseDI	CCNNGG	8 cut(s)	38, 144, 200, 239, 280, 444, 687, 1341
BseGI	GGATG	2 cut(s)	741, 964
BseLI	CCNNNNNNNGG	3 cut(s)	272, 1027, 1343
BseMI	GCAATG	1 cut(s)	64
BseMII	CTCAG	3 cut(s)	181, 638, 1089
BseNI	ACTGG	2 cut(s)	45, 1309
BseRI	GAGGAG	3 cut(s)	269, 1117, 1193
BseXI	GCAGC	2 cut(s)	42, 870
BseYI	CCCAGC	2 cut(s)	578, 1052
BsgI	GTGCAG	1 cut(s)	896
Bsh1236I	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
BshTI	ACCGGT	2 cut(s)	347, 1212
BsiHKAI	GWGCWC	1 cut(s)	1287
BslFI	GGGAC	6 cut(s)	85, 248, 547, 704, 832, 1121
BslI	CCNNNNNNNGG	3 cut(s)	272, 1027, 1343
BsmAI	GTCTC	3 cut(s)	327, 502, 939
BsmFI	GGGAC	6 cut(s)	85, 248, 547, 704, 832, 1121
Bso31I	GGTCTC	2 cut(s)	327, 939
Bsp1286I	GDGCHC	2 cut(s)	182, 1287
Bsp13I	TCCGGA	1 cut(s)	1279
Bsp1407I	TGTACA	3 cut(s)	342, 798, 1224
Bsp143I	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
Bsp1720I	GCTNAGC	2 cut(s)	167, 1075
Bsp19I	CCATGG	1 cut(s)	200
Bsp68I	TCGCGA	1 cut(s)	297
BspCNI	CTCAG	3 cut(s)	180, 639, 1088
BspEI	TCCGGA	1 cut(s)	1279
BspFNI	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
BspLI	GGNNCC	7 cut(s)	206, 264, 352, 692, 1010, 1058, 1235
BspPI	GGATC	5 cut(s)	68, 626, 1111, 1191, 1226
BspQI	GCTCTTC	1 cut(s)	461
BspTNI	GGTCTC	2 cut(s)	327, 939
BsrDI	GCAATG	1 cut(s)	64
BsrFI	RCCGGY	5 cut(s)	347, 803, 1156, 1212, 1368
BsrGI	TGTACA	3 cut(s)	342, 798, 1224
BsrI	ACTGG	2 cut(s)	45, 1309
BssAI	RCCGGY	5 cut(s)	347, 803, 1156, 1212, 1368
BssECI	CCNNGG	8 cut(s)	38, 144, 200, 239, 280, 444, 687, 1341
BssMI	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
BssNI	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
BssT1I	CCWWGG	2 cut(s)	38, 200
Bst2UI	CCWGG	5 cut(s)	470, 688, 1061, 1238, 1379
Bst4CI	ACNGT	4 cut(s)	137, 823, 1229, 1338
Bst6I	CTCTTC	3 cut(s)	461, 505, 1360
BstACI	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	883
BstAUI	TGTACA	3 cut(s)	342, 798, 1224
BstBAI	YACGTR	1 cut(s)	1125
BstC8I	GCNNGC	5 cut(s)	698, 781, 1029, 1144, 1275
BstDEI	CTNAG	3 cut(s)	167, 647, 1075
BstDSI	CCRYGG	3 cut(s)	144, 200, 239
BstEII	GGTNACC	2 cut(s)	464, 904
BstF5I	GGATG	2 cut(s)	741, 964
BstFNI	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
BstH2I	RGCGCY	2 cut(s)	144, 614
BstHHI	GCGC	7 cut(s)	143, 530, 613, 751, 809, 926, 984
BstKTI	GATC	6 cut(s)	76, 621, 1119, 1186, 1210, 1221
BstMAI	GTCTC	3 cut(s)	327, 502, 939
BstMBI	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
BstNI	CCWGG	5 cut(s)	470, 688, 1061, 1238, 1379
BstPI	GGTNACC	2 cut(s)	464, 904
BstSCI	CCNGG	8 cut(s)	161, 443, 468, 530, 686, 1059, 1236, 1377
BstUI	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
BstV1I	GCAGC	2 cut(s)	42, 870
BstV2I	GAAGAC	2 cut(s)	261, 666
BtgI	CCRYGG	3 cut(s)	144, 200, 239
BtgZI	GCGATG	1 cut(s)	110
BtsCI	GGATG	2 cut(s)	741, 964
BtsIMutI	CAGTG	2 cut(s)	1234, 1390
BtuMI	TCGCGA	1 cut(s)	297
Cac8I	GCNNGC	5 cut(s)	698, 781, 1029, 1144, 1275
CaiI	CAGNNNCTG	1 cut(s)	883
CfoI	GCGC	7 cut(s)	143, 530, 613, 751, 809, 926, 984
Cfr10I	RCCGGY	5 cut(s)	347, 803, 1156, 1212, 1368
Cfr13I	GGNCC	8 cut(s)	42, 262, 691, 1009, 1018, 1144, 1154, 1371
Cfr42I	CCGCGG	1 cut(s)	147
CpoI	CGGWCCG	1 cut(s)	1371
CseI	GACGC	6 cut(s)	253, 641, 767, 889, 915, 1331
Csp6I	GTAC	6 cut(s)	48, 343, 394, 565, 799, 1225
CspAI	ACCGGT	2 cut(s)	347, 1212
CspI	CGGWCCG	1 cut(s)	1371
CviAII	CATG	5 cut(s)	187, 201, 1001, 1294, 1300
CviQI	GTAC	6 cut(s)	48, 343, 394, 565, 799, 1225
DdeI	CTNAG	3 cut(s)	167, 647, 1075
DpnI	GATC	6 cut(s)	75, 620, 1118, 1185, 1209, 1220
DpnII	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
EaeI	YGGCCR	3 cut(s)	777, 1170, 1375
Eam1104I	CTCTTC	3 cut(s)	461, 505, 1360
EarI	CTCTTC	3 cut(s)	461, 505, 1360
EciI	GGCGGA	1 cut(s)	827
Ecl136II	GAGCTC	1 cut(s)	1285
Eco130I	CCWWGG	2 cut(s)	38, 200
Eco24I	GRGCYC	2 cut(s)	182, 1287
Eco31I	GGTCTC	2 cut(s)	327, 939
Eco47I	GGWCC	4 cut(s)	262, 691, 1009, 1371
Eco47III	AGCGCT	1 cut(s)	612
Eco53kI	GAGCTC	1 cut(s)	1285
Eco57I	CTGAAG	1 cut(s)	908
Eco72I	CACGTG	1 cut(s)	1125
Eco91I	GGTNACC	2 cut(s)	464, 904
EcoICRI	GAGCTC	1 cut(s)	1285
EcoO109I	RGGNCCY	2 cut(s)	262, 1009
EcoO65I	GGTNACC	2 cut(s)	464, 904
EcoRII	CCWGG	5 cut(s)	468, 686, 1059, 1236, 1377
EcoT14I	CCWWGG	2 cut(s)	38, 200
EcoT22I	ATGCAT	1 cut(s)	734
EcoT38I	GRGCYC	2 cut(s)	182, 1287
ErhI	CCWWGG	2 cut(s)	38, 200
FaeI	CATG	5 cut(s)	190, 204, 1004, 1297, 1303
FalI	AAGNNNNNCTT	2 cut(s)	1339, 1371
FaqI	GGGAC	6 cut(s)	85, 248, 547, 704, 832, 1121
FatI	CATG	5 cut(s)	186, 200, 1000, 1293, 1299
FauI	CCCGC	1 cut(s)	1135
FauNDI	CATATG	1 cut(s)	1091
FokI	GGATG	2 cut(s)	728, 971
FriOI	GRGCYC	2 cut(s)	182, 1287
FspBI	CTAG	1 cut(s)	39
GlaI	GCGC	7 cut(s)	142, 529, 612, 750, 808, 925, 983
GsaI	CCCAGC	2 cut(s)	582, 1056
HaeII	RGCGCY	2 cut(s)	144, 614
HgaI	GACGC	6 cut(s)	253, 641, 767, 889, 915, 1331
HhaI	GCGC	7 cut(s)	143, 530, 613, 751, 809, 926, 984
Hin1I	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
Hin1II	CATG	5 cut(s)	190, 204, 1004, 1297, 1303
Hin6I	GCGC	7 cut(s)	141, 528, 611, 749, 807, 924, 982
HinP1I	GCGC	7 cut(s)	141, 528, 611, 749, 807, 924, 982
HincII	GTYRAC	1 cut(s)	766
HindII	GTYRAC	1 cut(s)	766
HinfI	GANTC	5 cut(s)	382, 495, 544, 737, 1328
HpaI	GTTAAC	1 cut(s)	766
HphI	GGTGA	9 cut(s)	189, 220, 436, 458, 835, 916, 931, 1171, 1202
Hpy166II	GTNNAC	8 cut(s)	228, 243, 345, 520, 766, 801, 1190, 1225
Hpy188I	TCNGA	9 cut(s)	73, 355, 371, 549, 631, 648, 958, 1270, 1342
Hpy188III	TCNNGA	4 cut(s)	116, 296, 1187, 1280
Hpy8I	GTNNAC	8 cut(s)	228, 243, 345, 520, 766, 801, 1190, 1225
Hpy99I	CGWCG	7 cut(s)	110, 635, 758, 761, 776, 958, 1325
HpyAV	CCTTC	8 cut(s)	160, 296, 347, 782, 820, 832, 1246, 1380
HpyCH4III	ACNGT	4 cut(s)	137, 823, 1229, 1338
HpyCH4IV	ACGT	3 cut(s)	108, 1124, 1192
HpyCH4V	TGCA	3 cut(s)	732, 877, 1303
HpyF3I	CTNAG	3 cut(s)	167, 647, 1075
HpySE526I	ACGT	3 cut(s)	108, 1124, 1192
Hsp92I	GRCGYC	5 cut(s)	108, 245, 633, 759, 1323
Hsp92II	CATG	5 cut(s)	190, 204, 1004, 1297, 1303
HspAI	GCGC	7 cut(s)	141, 528, 611, 749, 807, 924, 982
Kpn2I	TCCGGA	1 cut(s)	1279
KspAI	GTTAAC	1 cut(s)	766
KspI	CCGCGG	1 cut(s)	147
Kzo9I	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
LguI	GCTCTTC	1 cut(s)	461
LmnI	GCTCC	7 cut(s)	841, 979, 1056, 1233, 1270, 1282, 1282
Lsp1109I	GCAGC	2 cut(s)	42, 870
LweI	GCATC	2 cut(s)	528, 741
MaeI	CTAG	1 cut(s)	39
MaeII	ACGT	3 cut(s)	108, 1124, 1192
MaeIII	GTNAC	3 cut(s)	195, 464, 904
MalI	GATC	6 cut(s)	75, 620, 1118, 1185, 1209, 1220
MboI	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
MboII	GAAGA	9 cut(s)	266, 391, 448, 492, 671, 847, 865, 1347, 1350
MfeI	CAATTG	1 cut(s)	432
MhlI	GDGCHC	2 cut(s)	182, 1287
MlsI	TGGCCA	1 cut(s)	779
MluCI	AATT	2 cut(s)	432, 970
MluNI	TGGCCA	1 cut(s)	779
MlyI	GAGTC	4 cut(s)	504, 538, 746, 1322
MmeI	TCCRAC	4 cut(s)	378, 654, 936, 1248
Mox20I	TGGCCA	1 cut(s)	779
Mph1103I	ATGCAT	1 cut(s)	734
MroI	TCCGGA	1 cut(s)	1279
MroXI	GAANNNNTTC	1 cut(s)	455
MscI	TGGCCA	1 cut(s)	779
MseI	TTAA	2 cut(s)	765, 969
MslI	CAYNNNNRTG	4 cut(s)	191, 1094, 1292, 1298
Msp20I	TGGCCA	1 cut(s)	779
MspA1I	CMGCKG	6 cut(s)	146, 171, 399, 417, 1071, 1079
MspR9I	CCNGG	8 cut(s)	163, 445, 470, 532, 688, 1061, 1238, 1379
MunI	CAATTG	1 cut(s)	432
MvaI	CCWGG	5 cut(s)	470, 688, 1061, 1238, 1379
MvnI	CGCG	9 cut(s)	95, 146, 297, 528, 749, 809, 917, 926, 984
NciI	CCSGG	3 cut(s)	163, 445, 532
NcoI	CCATGG	1 cut(s)	200
NdeI	CATATG	1 cut(s)	1091
NdeII	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
NlaIII	CATG	5 cut(s)	190, 204, 1004, 1297, 1303
NlaIV	GGNNCC	7 cut(s)	206, 264, 352, 692, 1010, 1058, 1235
NmeAIII	GCCGAG	1 cut(s)	261
NmuCI	GTSAC	3 cut(s)	195, 464, 904
NruI	TCGCGA	1 cut(s)	297
NsiI	ATGCAT	1 cut(s)	734
PciSI	GCTCTTC	1 cut(s)	461
PcsI	WCGNNNNNNNCGW	6 cut(s)	60, 92, 114, 753, 759, 780
PdmI	GAANNNNTTC	1 cut(s)	455
PfeI	GAWTC	1 cut(s)	382
PflFI	GACNNNGTC	2 cut(s)	193, 260
PflMI	CCANNNNNTGG	1 cut(s)	1027
PinAI	ACCGGT	2 cut(s)	347, 1212
PleI	GAGTC	4 cut(s)	503, 538, 745, 1322
PmaCI	CACGTG	1 cut(s)	1125
PmlI	CACGTG	1 cut(s)	1125
PpsI	GAGTC	4 cut(s)	503, 538, 745, 1322
Ppu21I	YACGTR	1 cut(s)	1125
PpuMI	RGGWCCY	2 cut(s)	262, 1009
Psp124BI	GAGCTC	1 cut(s)	1287
Psp1406I	AACGTT	1 cut(s)	1192
Psp5II	RGGWCCY	2 cut(s)	262, 1009
Psp6I	CCWGG	5 cut(s)	468, 686, 1059, 1236, 1377
PspCI	CACGTG	1 cut(s)	1125
PspEI	GGTNACC	2 cut(s)	464, 904
PspFI	CCCAGC	2 cut(s)	578, 1052
PspGI	CCWGG	5 cut(s)	468, 686, 1059, 1236, 1377
PspN4I	GGNNCC	7 cut(s)	206, 264, 352, 692, 1010, 1058, 1235
PspPI	GGNCC	8 cut(s)	42, 262, 691, 1009, 1018, 1144, 1154, 1371
PspPPI	RGGWCCY	2 cut(s)	262, 1009
PstNI	CAGNNNCTG	1 cut(s)	883
PsyI	GACNNNGTC	2 cut(s)	193, 260
PvuII	CAGCTG	1 cut(s)	1079
RruI	TCGCGA	1 cut(s)	297
RsaI	GTAC	6 cut(s)	49, 344, 395, 566, 800, 1226
RsaNI	GTAC	6 cut(s)	48, 343, 394, 565, 799, 1225
RseI	CAYNNNNRTG	4 cut(s)	191, 1094, 1292, 1298
Rsr2I	CGGWCCG	1 cut(s)	1371
RsrII	CGGWCCG	1 cut(s)	1371
SacI	GAGCTC	1 cut(s)	1287
SacII	CCGCGG	1 cut(s)	147
SapI	GCTCTTC	1 cut(s)	461
SaqAI	TTAA	2 cut(s)	765, 969
Sau3AI	GATC	6 cut(s)	73, 618, 1116, 1183, 1207, 1218
Sau96I	GGNCC	8 cut(s)	42, 262, 691, 1009, 1018, 1144, 1154, 1371
ScaI	AGTACT	2 cut(s)	49, 566
SchI	GAGTC	4 cut(s)	504, 538, 746, 1322
ScrFI	CCNGG	8 cut(s)	163, 445, 470, 532, 688, 1061, 1238, 1379
SduI	GDGCHC	2 cut(s)	182, 1287
SfaNI	GCATC	2 cut(s)	528, 741
Sfr303I	CCGCGG	1 cut(s)	147
SgrAI	CRCCGGYG	2 cut(s)	803, 1212
SgrBI	CCGCGG	1 cut(s)	147
SinI	GGWCC	4 cut(s)	262, 691, 1009, 1371
SmiMI	CAYNNNNRTG	4 cut(s)	191, 1094, 1292, 1298
SmlI	CTYRAG	3 cut(s)	386, 652, 1013
SmoI	CTYRAG	3 cut(s)	386, 652, 1013
Sse9I	AATT	2 cut(s)	432, 970
SspI	AATATT	1 cut(s)	82
SspMI	CTAG	1 cut(s)	39
SstI	GAGCTC	1 cut(s)	1287
StyD4I	CCNGG	8 cut(s)	161, 443, 468, 530, 686, 1059, 1236, 1377
StyI	CCWWGG	2 cut(s)	38, 200
TaaI	ACNGT	4 cut(s)	137, 823, 1229, 1338
TaiI	ACGT	3 cut(s)	111, 1127, 1195
TaqI	TCGA	5 cut(s)	153, 717, 735, 1196, 1361
TasI	AATT	2 cut(s)	432, 970
TatI	WGTACW	6 cut(s)	47, 342, 393, 564, 798, 1224
TfiI	GAWTC	1 cut(s)	382
Tru1I	TTAA	2 cut(s)	765, 969
Tru9I	TTAA	2 cut(s)	765, 969
TscAI	CASTG	1 cut(s)	1234
TseFI	GTSAC	3 cut(s)	195, 464, 904
TseI	GCWGC	2 cut(s)	30, 883
Tsp45I	GTSAC	3 cut(s)	195, 464, 904
TspGWI	ACGGA	3 cut(s)	213, 559, 760
TspRI	CASTG	1 cut(s)	1234
Tth111I	GACNNNGTC	2 cut(s)	193, 260
Van91I	CCANNNNNTGG	1 cut(s)	1027
VpaK11BI	GGWCC	4 cut(s)	262, 691, 1009, 1371
XmaJI	CCTAGG	1 cut(s)	38
XmnI	GAANNNNTTC	1 cut(s)	455
XspI	CTAG	1 cut(s)	39
ZraI	GACGTC	1 cut(s)	109
ZrmI	AGTACT	2 cut(s)	49, 566
Zsp2I	ATGCAT	1 cut(s)	734

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.