FvH4_3g04900

CBS domain-containing protein CBSX5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016282
Physical Location & Seq
Forward (+)
2830884 .. 2832661
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04900.t1

Sequence Viewer

Length: 1191 bp
ATGTCACAACCGCGGAGTTTGTTTTCGCATGAGGTGGCCGATCTGTGCCTGGGGAAGCCCCCGCTGAAGTCGGTTTCGATCGCCGCCACCGTCGGCGAGGCGTTGTCAGCGCTGAAGAGGCTCGGCGAGACCTTCCTCAGCGTGTGGAGCTGCAACCACGCTGAGAGGAACAACGACGTCATTGTGGAGGAGGAGTGTCGGTGCGTAGGGAAGGTTTGCATGCTCGATGTGATTGCCTATTTGGGGAAAGAAGAGAACCGTTCGTCTCCGGCCAAAGCCCTCGAGGCTCCGCTTAAGGTTTTGATTCTCAGGGAACCCGCCACCGCCCTTGTTAGGCATTTGGAATCACACGCCAGCTTGGTGGAGGCTATGGATTTGATTCTGGAAGGTGCACAGAATCTTGTGATACCATTGGAAAGCCGGAGGAACACAGGCTTGAGGAACAAGCTTCTCCACAGCAACTCCTTCAACCCCACCGTCCACAACAACCGGGAATACTGCTGGCTCACACAAGAAGACATTGTCCGGTACCTACTCAACTCGATTGGGTACTTTAGCCATGTATCGAACACCGCCATCAACACCCTCCACATCATTGACTCCATAAACATGCTCGCAGTCCATTATGATGACCCTGCCTCAGCTGCATTACCTCTGATCTCTCAGTCCCTTGTGCAGCAAACTTCAGTTGCTATTCTTGATGGGGAAGGCATTGTGATCGGGGAGATCTCGCCTTATACCCTCAACACCTTCCACGAGTCGGTGGCAGCTGCGATCGTGACGCTATCTGCTGGGGAACTGATGGCGTACATTGACTGTGGTGGTCCGTGGGATGACCAAGTGCAGCTGGTGAAAGACAGGTTGCTGGAGAAGAAGTATGAGGGGTTGTTGGAGTTGATGGAAGAAGACTTCACATTCTCGTCTTCTTCTTCCATATGTTCAACTTCATCGGACGAAGAGTTTGGCCAGGGAAGGAGTTGGAGGCTAGGAGGGTACTCGGCAAGGCTGGTGAGGAGATCAGAGGCAATCGTGTGCTATCCATGCAGCTCATTGGTGGCTGTGATGGTTCAGGCTCTTTCTCATCGTGTGAGTTATGTGTGGGTTGTTGATGAGGATAGAACCTTGTCTGGTATTGTTACCTTTGCAAGCATGTTCAAAATTTTTCGGGATCGTTTGAGGTTCATGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

397

Amino Acids

43.86

Weight (kDa)

5.36

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04900
malus_domestica MD05G1314900.v1.1 MD10G1296300.v1.1
prunus_persica Prupe.4G044300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0008061
rosa_laevigata RLG00000031532
rosa_multiflora Rmu_sc0001626.1_g000001 Rmu_sc0017465.1_g000002
rosa_roxburghii Rroxscaffold_1G00068420
rosa_rugosa Rorug04G0433100
rosa_samantha Rh5AG061100 Rh5BG057900 Rh5CG068000 Rh5DG057100
rosa_wichuraiana Rw5G005950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AbsI CCTCGAGG 1 cut(s) 281
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AccII CGCG 1 cut(s) 13
AciI CCGC 8 cut(s) 11, 13, 62, 84, 290, 318, 324, 573
AclWI GGATC 1 cut(s) 1176
AcoI YGGCCR 3 cut(s) 36, 270, 964
AcsI RAATTY 1 cut(s) 1158
AcuI CTGAAG 3 cut(s) 86, 134, 669
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 4 cut(s) 530, 551, 809, 995
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 3 cut(s) 243, 333, 760
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 3 cut(s) 469, 942, 1156
AjnI CCWGG 2 cut(s) 48, 966
AluBI AGCT 7 cut(s) 150, 357, 448, 644, 770, 847, 1047
AluI AGCT 7 cut(s) 150, 357, 448, 644, 770, 847, 1047
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 122, 270
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 1 cut(s) 1176
Ama87I CYCGRG 1 cut(s) 281
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 3 cut(s) 36, 270, 964
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 7 cut(s) 150, 644, 676, 767, 770, 844, 1044
ApoI RAATTY 1 cut(s) 1158
ArsI GACNNNNNNTTYG 2 cut(s) 944, 976
Asp718I GGTACC 1 cut(s) 528
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 824
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 2 cut(s) 862, 1021
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 966
BanI GGYRCC 1 cut(s) 528
BauI CACGAG 1 cut(s) 755
BbsI GAAGAC 3 cut(s) 522, 912, 915
Bbv12I GWGCWC 1 cut(s) 394
BbvCI CCTCAGC 2 cut(s) 137, 640
BbvI GCAGC 7 cut(s) 137, 631, 688, 757, 779, 856, 1056
BccI CCATC 5 cut(s) 584, 695, 796, 892, 1057
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 700, 734
BciT130I CCWGG 2 cut(s) 50, 968
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 122, 270
BfaI CTAG 1 cut(s) 986
BfoI RGCGCY 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 726
BisI GCNGC 8 cut(s) 84, 151, 645, 677, 768, 771, 845, 1045
BlsI GCNGC 8 cut(s) 85, 152, 646, 678, 769, 772, 846, 1046
Bme1390I CCNGG 3 cut(s) 50, 491, 968
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 3 cut(s) 288, 315, 530
BmrFI CCNGG 3 cut(s) 50, 491, 968
BpiI GAAGAC 3 cut(s) 522, 912, 915
BpmI CTGGAG 1 cut(s) 887
Bpu10I CCTNAGC 2 cut(s) 137, 640
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 4 cut(s) 11, 49, 827, 967
BsaWI WCCGGW 1 cut(s) 525
BsaXI ACNNNNNCTCC 8 cut(s) 179, 185, 209, 215, 446, 476, 860, 890
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 333, 760
BseBI CCWGG 2 cut(s) 50, 968
BseDI CCNNGG 4 cut(s) 11, 49, 827, 967
BseGI GGATG 1 cut(s) 838
BseLI CCNNNNNNNGG 3 cut(s) 243, 333, 760
BseMII CTCAG 5 cut(s) 151, 153, 322, 654, 677
BseRI GAGGAG 3 cut(s) 203, 206, 1027
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 7 cut(s) 137, 631, 688, 757, 779, 856, 1056
BseYI CCCAGC 1 cut(s) 791
BsgI GTGCAG 2 cut(s) 695, 863
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 2 cut(s) 81, 777
BshFI GGCC 3 cut(s) 38, 272, 966
BshNI GGYRCC 1 cut(s) 528
BsiEI CGRYCG 2 cut(s) 81, 777
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 4 cut(s) 269, 421, 490, 526
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 3 cut(s) 243, 333, 760
BsmAI GTCTC 2 cut(s) 122, 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 652
BsnI GGCC 3 cut(s) 38, 272, 966
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
BspACI CCGC 8 cut(s) 11, 13, 62, 84, 290, 318, 324, 573
BspANI GGCC 3 cut(s) 38, 272, 966
BspCNI CTCAG 5 cut(s) 150, 154, 321, 653, 676
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 3 cut(s) 288, 315, 530
BspPI GGATC 1 cut(s) 1176
BspT107I GGYRCC 1 cut(s) 528
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 11, 49, 827, 967
BssMI GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 755
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 2 cut(s) 50, 968
Bst4CI ACNGT 4 cut(s) 91, 260, 478, 818
Bst6I CTCTTC 3 cut(s) 110, 246, 951
BstACI GRCGYC 1 cut(s) 177
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 5 cut(s) 221, 355, 503, 615, 1147
BstDEI CTNAG 5 cut(s) 137, 162, 308, 640, 663
BstDSI CCRYGG 2 cut(s) 11, 827
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 8 cut(s) 43, 81, 660, 720, 729, 777, 1019, 1171
BstMAI GTCTC 2 cut(s) 122, 270
BstMBI GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
BstMCI CGRYCG 2 cut(s) 81, 777
BstMWI GCNNNNNNNGC 6 cut(s) 107, 118, 147, 284, 644, 1041
BstNI CCWGG 2 cut(s) 50, 968
BstNSI RCATGY 3 cut(s) 223, 613, 1153
BstSCI CCNGG 3 cut(s) 48, 489, 966
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 7 cut(s) 137, 631, 688, 757, 779, 856, 1056
BstV2I GAAGAC 3 cut(s) 522, 912, 915
BstX2I RGATCY 1 cut(s) 726
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 1 cut(s) 726
BsuRI GGCC 3 cut(s) 38, 272, 966
BtgI CCRYGG 2 cut(s) 11, 827
BtsCI GGATG 1 cut(s) 838
Cac8I GCNNGC 5 cut(s) 221, 355, 503, 615, 1147
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 824
Cfr42I CCGCGG 1 cut(s) 14
CseI GACGC 1 cut(s) 790
Csp6I GTAC 4 cut(s) 529, 550, 808, 994
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 7 cut(s) 29, 220, 560, 610, 1041, 1150, 1183
CviQI GTAC 4 cut(s) 529, 550, 808, 994
DdeI CTNAG 5 cut(s) 137, 162, 308, 640, 663
DpnI GATC 8 cut(s) 42, 80, 659, 719, 728, 776, 1018, 1170
DpnII GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
EaeI YGGCCR 3 cut(s) 36, 270, 964
Eam1104I CTCTTC 3 cut(s) 110, 246, 951
EarI CTCTTC 3 cut(s) 110, 246, 951
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 824
Eco47III AGCGCT 1 cut(s) 111
Eco57I CTGAAG 3 cut(s) 86, 134, 669
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 48, 966
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 7 cut(s) 32, 223, 563, 613, 1044, 1153, 1186
FaqI GGGAC 1 cut(s) 652
FatI CATG 7 cut(s) 28, 219, 559, 609, 1040, 1149, 1182
FauI CCCGC 2 cut(s) 69, 325
FauNDI CATATG 1 cut(s) 935
Fnu4HI GCNGC 8 cut(s) 84, 151, 645, 677, 768, 771, 845, 1045
FokI GGATG 1 cut(s) 845
Fsp4HI GCNGC 8 cut(s) 84, 151, 645, 677, 768, 771, 845, 1045
FspBI CTAG 1 cut(s) 986
GlaI GCGC 1 cut(s) 111
GluI GCNGC 8 cut(s) 84, 151, 645, 677, 768, 771, 845, 1045
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 887
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 3 cut(s) 38, 272, 966
HapII CCGG 4 cut(s) 269, 421, 490, 526
HgaI GACGC 1 cut(s) 790
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 7 cut(s) 32, 223, 563, 613, 1044, 1153, 1186
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 6 cut(s) 304, 344, 379, 397, 599, 758
HpaII CCGG 4 cut(s) 269, 421, 490, 526
HphI GGTGA 2 cut(s) 862, 1021
Hpy166II GTNNAC 2 cut(s) 392, 481
Hpy188I TCNGA 3 cut(s) 657, 952, 1021
Hpy188III TCNNGA 4 cut(s) 383, 698, 778, 1166
Hpy8I GTNNAC 2 cut(s) 392, 481
Hpy99I CGWCG 2 cut(s) 95, 179
HpyAV CCTTC 7 cut(s) 142, 205, 380, 475, 701, 760, 966
HpyCH4III ACNGT 4 cut(s) 91, 260, 478, 818
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 8 cut(s) 153, 219, 392, 647, 676, 844, 1044, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 118, 147, 284, 644, 1041
HpyF3I CTNAG 5 cut(s) 137, 162, 308, 640, 663
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 7 cut(s) 32, 223, 563, 613, 1044, 1153, 1186
HspAI GCGC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 532
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
LmnI GCTCC 2 cut(s) 147, 292
Lsp1109I GCAGC 7 cut(s) 137, 631, 688, 757, 779, 856, 1056
MaeI CTAG 1 cut(s) 986
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 3, 778, 1135
MalI GATC 8 cut(s) 42, 80, 659, 719, 728, 776, 1018, 1170
MboI GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
MflI RGATCY 1 cut(s) 726
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 966
MluCI AATT 1 cut(s) 1158
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 2 cut(s) 593, 767
MmeI TCCRAC 2 cut(s) 870, 959
Mox20I TGGCCA 1 cut(s) 966
MscI TGGCCA 1 cut(s) 966
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 2 cut(s) 608, 627
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 5 cut(s) 13, 64, 644, 770, 847
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 4 cut(s) 269, 421, 490, 526
MspR9I CCNGG 3 cut(s) 50, 491, 968
MvaI CCWGG 2 cut(s) 50, 968
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 6 cut(s) 107, 118, 147, 284, 644, 1041
NciI CCSGG 1 cut(s) 491
NdeI CATATG 1 cut(s) 935
NdeII GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
NlaIII CATG 7 cut(s) 32, 223, 563, 613, 1044, 1153, 1186
NlaIV GGNNCC 3 cut(s) 288, 315, 530
NmeAIII GCCGAG 2 cut(s) 102, 977
NmuCI GTSAC 2 cut(s) 3, 778
NspI RCATGY 3 cut(s) 223, 613, 1153
PaeI GCATGC 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 4 cut(s) 304, 344, 379, 397
PflFI GACNNNGTC 1 cut(s) 521
PkrI GCNGC 8 cut(s) 85, 152, 646, 678, 769, 772, 846, 1046
Ple19I CGATCG 2 cut(s) 81, 777
PleI GAGTC 2 cut(s) 593, 766
PpsI GAGTC 2 cut(s) 593, 766
Psp6I CCWGG 2 cut(s) 48, 966
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 48, 966
PspN4I GGNNCC 3 cut(s) 288, 315, 530
PspPI GGNCC 1 cut(s) 824
PspXI VCTCGAGB 1 cut(s) 281
PsuI RGATCY 1 cut(s) 726
PsyI GACNNNGTC 1 cut(s) 521
PvuI CGATCG 2 cut(s) 81, 777
PvuII CAGCTG 3 cut(s) 644, 770, 847
RsaI GTAC 4 cut(s) 530, 551, 809, 995
RsaNI GTAC 4 cut(s) 529, 550, 808, 994
RseI CAYNNNNRTG 2 cut(s) 608, 627
SacII CCGCGG 1 cut(s) 14
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 8 cut(s) 84, 151, 645, 677, 768, 771, 845, 1045
Sau3AI GATC 8 cut(s) 40, 78, 657, 717, 726, 774, 1016, 1168
Sau96I GGNCC 1 cut(s) 824
SchI GAGTC 2 cut(s) 593, 767
ScrFI CCNGG 3 cut(s) 50, 491, 968
SduI GDGCHC 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 281
Sfr303I CCGCGG 1 cut(s) 14
SgrBI CCGCGG 1 cut(s) 14
SinI GGWCC 1 cut(s) 824
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 608, 627
SmlI CTYRAG 3 cut(s) 281, 293, 436
SmoI CTYRAG 3 cut(s) 281, 293, 436
SphI GCATGC 1 cut(s) 223
Sse9I AATT 1 cut(s) 1158
SsiI CCGC 8 cut(s) 11, 13, 62, 84, 290, 318, 324, 573
SspMI CTAG 1 cut(s) 986
StyD4I CCNGG 3 cut(s) 48, 489, 966
TaaI ACNGT 4 cut(s) 91, 260, 478, 818
TaiI ACGT 1 cut(s) 180
TaqI TCGA 5 cut(s) 77, 225, 282, 542, 566
TasI AATT 1 cut(s) 1158
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 4 cut(s) 304, 344, 379, 397
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseFI GTSAC 2 cut(s) 3, 778
TseI GCWGC 7 cut(s) 150, 644, 676, 767, 770, 844, 1044
Tsp45I GTSAC 2 cut(s) 3, 778
TspDTI ATGAA 2 cut(s) 936, 1171
TspGWI ACGGA 1 cut(s) 816
Tth111I GACNNNGTC 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 1 cut(s) 1158
XceI RCATGY 3 cut(s) 223, 613, 1153
XhoI CTCGAG 1 cut(s) 281
XspI CTAG 1 cut(s) 986
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.