Rw5G005950

CBS domain-containing protein CBSX5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016282
Physical Location & Seq
Reverse (-)
6165329 .. 6166819
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005950.1

Sequence Viewer

Length: 1092 bp
ATGTCACCAACTCGGAATTTGTTTTCGCATGAGGTCGCGGATCTGTGCATCGGGAAGCCGGCGCTGAAGTCGCTGTCGGTCGCCGCCACGGTAGGCGAGGCATTGTCGGCGCTGAAGAGGCTCGGCGAGACCTTCCTCAGCGTGTGGAGCTGCAACCATTCCGAGAAGAACGACGTCGTTGCGGAGGACGAGTGCCGGTGCGTCGGGAAGGTTTGCATGCTCGATGTGATCGTTTATTTGGGGAAAGAAGAGAACCTGTCGTCTCCGGCCAAAGCGCTCGAGGCTCCGATCGAGGCTATAGATTTGATTCTGGAAGGTGCACAGAATCTTGTGATACCACTCGAAAGCAGGAGGAACACAGGCTCGAGAAAACAGCTTCTCCACACTAACTCCTTCAACCTGACTGTCCACAACAACAGGGAATACTGCTGGCTCACCCAAGAAGATGTTATCCGCTACCTACTGAACTCGATTGGTGTCTTTAGCCACGTATCAAACAACCCCATCAACACCCTCCAGATCATCGACTCGAAAAACATGCTTGCGGTCCATTATGATGACCCTGCCTCAGCGGCATTACCTCTCATTTCTCAGTCCCTAGTTCAGCAAACTTCGGTTGCTATTCTTGATGGGGAAGGCAGGGTGATCGGGGAGATCTCGCCTTATACCCTCAACACCTGCCACGAGTCTGTGGCAGCTGCCATTGCGACACTTTCTGCTGGGGAACTGATGGCGTACATTGACTGTGGTGGTCCAACGGATGACCTAGTGCAGACGGTGAAAGACAGGTTGCAGGAGAAGAAGTATGAGGGGTTCTTGGAGTTGATGGAAGAAGACTTCACATTGTCGTCTTCTTCTTCCATCTGTTCAACTTCTTCGGACGAAGAGTTTGGCCAGGGAAGGAGTTGGAGGTCAGAGGCGATCGTGTGCTATCCATGGAGCTCATTGGTGGCAGTGATGGTTCAGGCTCTTTCTCATCGTGTGAGTTATGTGTGGGTTGCTGATGAGGATGGAACTTTGTCTGGTATTATCACCTTTGCAAGCATGTTCAAAGTTTTCCGGGACCGTTTGAGGTTTATGGGATACGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

363

Amino Acids

39.9

Weight (kDa)

4.89

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04900
malus_domestica MD05G1314900.v1.1 MD10G1296300.v1.1
prunus_persica Prupe.4G044300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0008061
rosa_laevigata RLG00000031532
rosa_multiflora Rmu_sc0001626.1_g000001 Rmu_sc0017465.1_g000002
rosa_roxburghii Rroxscaffold_1G00068420
rosa_rugosa Rorug04G0433100
rosa_samantha Rh5AG061100 Rh5BG057900 Rh5CG068000 Rh5DG057100
rosa_wichuraiana Rw5G005950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 686
AatII GACGTC 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 686
AccII CGCG 1 cut(s) 38
AciI CCGC 6 cut(s) 38, 84, 182, 454, 545, 572
AclWI GGATC 1 cut(s) 48
AcoI YGGCCR 2 cut(s) 267, 892
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 2 cut(s) 86, 134
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 737
AfeI AGCGCT 1 cut(s) 276
AgsI TTSAA 3 cut(s) 397, 870, 1051
AjnI CCWGG 1 cut(s) 894
AluBI AGCT 4 cut(s) 150, 376, 698, 942
AluI AGCT 4 cut(s) 150, 376, 698, 942
Alw21I GWGCWC 2 cut(s) 322, 944
Alw26I GTCTC 2 cut(s) 122, 267
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 2 cut(s) 278, 364
Aor51HI AGCGCT 1 cut(s) 276
AoxI GGCC 2 cut(s) 267, 892
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 3 cut(s) 150, 695, 698
ApoI RAATTY 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
AspLEI GCGC 3 cut(s) 64, 112, 277
AspS9I GGNCC 3 cut(s) 547, 752, 1063
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 4 cut(s) 427, 655, 790, 1024
AvaI CYCGRG 2 cut(s) 278, 364
AvaII GGWCC 3 cut(s) 547, 752, 1063
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 894
BanII GRGCYC 1 cut(s) 944
BauI CACGAG 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 840, 843
Bbv12I GWGCWC 2 cut(s) 322, 944
BbvCI CCTCAGC 2 cut(s) 137, 568
BbvI GCAGC 3 cut(s) 137, 685, 707
BccI CCATC 7 cut(s) 512, 623, 724, 820, 869, 952, 1004
BcgI CGANNNNNNTGC 6 cut(s) 161, 195, 520, 554, 628, 662
BciT130I CCWGG 1 cut(s) 896
BciVI GTATCC 1 cut(s) 1076
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 122, 267
BfaI CTAG 2 cut(s) 599, 767
BfmI CTRYAG 1 cut(s) 297
BfoI RGCGCY 3 cut(s) 65, 113, 278
BfuAI ACCTGC 1 cut(s) 686
BfuI GTATCC 1 cut(s) 1076
BglI GCCNNNNNGGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 654
BisI GCNGC 5 cut(s) 84, 151, 573, 696, 699
BlsI GCNGC 5 cut(s) 85, 152, 574, 697, 700
Bme1390I CCNGG 2 cut(s) 896, 1061
Bme18I GGWCC 3 cut(s) 547, 752, 1063
BmeT110I CYCGRG 2 cut(s) 278, 364
BmgT120I GGNCC 3 cut(s) 547, 752, 1063
BmiI GGNNCC 2 cut(s) 285, 1064
BmrFI CCNGG 2 cut(s) 896, 1061
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 2 cut(s) 840, 843
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 2 cut(s) 137, 568
BpuMI CCSGG 1 cut(s) 1061
BsaAI YACGTR 1 cut(s) 490
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 87, 895, 935
BsaXI ACNNNNNCTCC 8 cut(s) 176, 206, 363, 374, 393, 404, 788, 818
Bse118I RCCGGY 2 cut(s) 58, 195
Bse3DI GCAATG 1 cut(s) 702
BseBI CCWGG 1 cut(s) 896
BseDI CCNNGG 3 cut(s) 87, 895, 935
BseGI GGATG 2 cut(s) 766, 1015
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 3 cut(s) 151, 582, 605
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 3 cut(s) 137, 685, 707
BseYI CCCAGC 1 cut(s) 719
BsgI GTGCAG 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 3 cut(s) 81, 291, 924
BshFI GGCC 2 cut(s) 269, 894
BsiEI CGRYCG 3 cut(s) 81, 291, 924
BsiHKAI GWGCWC 2 cut(s) 322, 944
BsiHKCI CYCGRG 2 cut(s) 278, 364
BsiSI CCGG 4 cut(s) 59, 196, 266, 1060
BslFI GGGAC 2 cut(s) 580, 1076
BsmAI GTCTC 2 cut(s) 122, 267
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 2 cut(s) 580, 1076
BsnI GGCC 2 cut(s) 269, 894
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 2 cut(s) 278, 364
Bsp1286I GDGCHC 2 cut(s) 322, 944
Bsp143I GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
Bsp19I CCATGG 1 cut(s) 935
BspACI CCGC 6 cut(s) 38, 84, 182, 454, 545, 572
BspANI GGCC 2 cut(s) 269, 894
BspCNI CTCAG 3 cut(s) 150, 581, 604
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 285, 1064
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 702
BsrFI RCCGGY 2 cut(s) 58, 195
BssAI RCCGGY 2 cut(s) 58, 195
BssECI CCNNGG 3 cut(s) 87, 895, 935
BssMI GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 935
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 5 cut(s) 91, 406, 746, 778, 1067
Bst6I CTCTTC 3 cut(s) 110, 243, 879
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 490
BstC8I GCNNGC 5 cut(s) 60, 218, 431, 543, 1042
BstDEI CTNAG 3 cut(s) 137, 568, 591
BstDSI CCRYGG 2 cut(s) 87, 935
BstF5I GGATG 2 cut(s) 766, 1015
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 3 cut(s) 65, 113, 278
BstHHI GCGC 3 cut(s) 64, 112, 277
BstKTI GATC 7 cut(s) 43, 231, 291, 522, 648, 657, 924
BstMAI GTCTC 2 cut(s) 122, 267
BstMBI GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
BstMCI CGRYCG 3 cut(s) 81, 291, 924
BstMWI GCNNNNNNNGC 7 cut(s) 70, 107, 118, 147, 281, 572, 704
BstNI CCWGG 1 cut(s) 896
BstNSI RCATGY 3 cut(s) 220, 541, 1048
BstSCI CCNGG 2 cut(s) 894, 1059
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 3 cut(s) 137, 685, 707
BstV2I GAAGAC 2 cut(s) 840, 843
BstX2I RGATCY 2 cut(s) 40, 654
BstYI RGATCY 2 cut(s) 40, 654
BsuI GTATCC 1 cut(s) 1076
BsuRI GGCC 2 cut(s) 269, 894
BtgI CCRYGG 2 cut(s) 87, 935
BtsCI GGATG 2 cut(s) 766, 1015
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 1 cut(s) 960
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 5 cut(s) 60, 218, 431, 543, 1042
CfoI GCGC 3 cut(s) 64, 112, 277
Cfr10I RCCGGY 2 cut(s) 58, 195
Cfr13I GGNCC 3 cut(s) 547, 752, 1063
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 736
CviAII CATG 5 cut(s) 29, 217, 538, 936, 1045
CviQI GTAC 1 cut(s) 736
DdeI CTNAG 3 cut(s) 137, 568, 591
DpnI GATC 7 cut(s) 42, 230, 290, 521, 647, 656, 923
DpnII GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
EaeI YGGCCR 2 cut(s) 267, 892
Eam1104I CTCTTC 3 cut(s) 110, 243, 879
EarI CTCTTC 3 cut(s) 110, 243, 879
Ecl136II GAGCTC 1 cut(s) 942
Eco130I CCWWGG 1 cut(s) 935
Eco24I GRGCYC 1 cut(s) 944
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 3 cut(s) 547, 752, 1063
Eco47III AGCGCT 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 942
Eco57I CTGAAG 2 cut(s) 86, 134
Eco88I CYCGRG 2 cut(s) 278, 364
EcoICRI GAGCTC 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 935
EcoT38I GRGCYC 1 cut(s) 944
ErhI CCWWGG 1 cut(s) 935
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 5 cut(s) 32, 220, 541, 939, 1048
FaqI GGGAC 2 cut(s) 580, 1076
FatI CATG 5 cut(s) 28, 216, 537, 935, 1044
Fnu4HI GCNGC 5 cut(s) 84, 151, 573, 696, 699
FokI GGATG 2 cut(s) 773, 1022
FriOI GRGCYC 1 cut(s) 944
Fsp4HI GCNGC 5 cut(s) 84, 151, 573, 696, 699
FspBI CTAG 2 cut(s) 599, 767
GlaI GCGC 3 cut(s) 63, 111, 276
GluI GCNGC 5 cut(s) 84, 151, 573, 696, 699
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 3 cut(s) 65, 113, 278
HaeIII GGCC 2 cut(s) 269, 894
HapII CCGG 4 cut(s) 59, 196, 266, 1060
HgaI GACGC 1 cut(s) 190
HhaI GCGC 3 cut(s) 64, 112, 277
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 5 cut(s) 32, 220, 541, 939, 1048
Hin6I GCGC 3 cut(s) 62, 110, 275
HinP1I GCGC 3 cut(s) 62, 110, 275
HinfI GANTC 4 cut(s) 307, 325, 527, 686
HpaII CCGG 4 cut(s) 59, 196, 266, 1060
HphI GGTGA 4 cut(s) 427, 655, 790, 1024
Hpy166II GTNNAC 2 cut(s) 320, 409
Hpy188I TCNGA 5 cut(s) 15, 163, 288, 880, 916
Hpy188III TCNNGA 6 cut(s) 52, 205, 311, 366, 517, 626
Hpy8I GTNNAC 2 cut(s) 320, 409
Hpy99I CGWCG 3 cut(s) 176, 179, 206
HpyAV CCTTC 6 cut(s) 142, 202, 308, 403, 629, 894
HpyCH4III ACNGT 5 cut(s) 91, 406, 746, 778, 1067
HpyCH4IV ACGT 2 cut(s) 174, 489
HpyCH4V TGCA 7 cut(s) 48, 153, 216, 320, 772, 793, 1040
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 107, 118, 147, 281, 572, 704
HpyF3I CTNAG 3 cut(s) 137, 568, 591
HpySE526I ACGT 2 cut(s) 174, 489
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 5 cut(s) 32, 220, 541, 939, 1048
HspAI GCGC 3 cut(s) 62, 110, 275
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Kzo9I GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
LmnI GCTCC 3 cut(s) 147, 289, 939
Lsp1109I GCAGC 3 cut(s) 137, 685, 707
LweI GCATC 1 cut(s) 57
MaeI CTAG 2 cut(s) 599, 767
MaeII ACGT 2 cut(s) 174, 489
MaeIII GTNAC 1 cut(s) 3
MalI GATC 7 cut(s) 42, 230, 290, 521, 647, 656, 923
MboI GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
MflI RGATCY 2 cut(s) 40, 654
MhlI GDGCHC 2 cut(s) 322, 944
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 1 cut(s) 16
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 2 cut(s) 521, 695
MmeI TCCRAC 2 cut(s) 779, 887
Mox20I TGGCCA 1 cut(s) 894
MroNI GCCGGC 1 cut(s) 58
MscI TGGCCA 1 cut(s) 894
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 894
MspA1I CMGCKG 2 cut(s) 572, 698
MspI CCGG 4 cut(s) 59, 196, 266, 1060
MspR9I CCNGG 2 cut(s) 896, 1061
MvaI CCWGG 1 cut(s) 896
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 7 cut(s) 70, 107, 118, 147, 281, 572, 704
NaeI GCCGGC 1 cut(s) 60
NciI CCSGG 1 cut(s) 1061
NcoI CCATGG 1 cut(s) 935
NdeII GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 5 cut(s) 32, 220, 541, 939, 1048
NlaIV GGNNCC 2 cut(s) 285, 1064
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 3 cut(s) 220, 541, 1048
PaeI GCATGC 1 cut(s) 220
PaeR7I CTCGAG 2 cut(s) 278, 364
PaqCI CACCTGC 1 cut(s) 686
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 2 cut(s) 307, 325
PfoI TCCNGGA 1 cut(s) 1059
PkrI GCNGC 5 cut(s) 85, 152, 574, 697, 700
Ple19I CGATCG 2 cut(s) 291, 924
PleI GAGTC 2 cut(s) 521, 694
PpsI GAGTC 2 cut(s) 521, 694
Ppu21I YACGTR 1 cut(s) 490
Psp124BI GAGCTC 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 2 cut(s) 285, 1064
PspPI GGNCC 3 cut(s) 547, 752, 1063
PspXI VCTCGAGB 1 cut(s) 278
PsrI GAACNNNNNNTAC 2 cut(s) 797, 829
PsuI RGATCY 2 cut(s) 40, 654
PvuI CGATCG 2 cut(s) 291, 924
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
RseI CAYNNNNRTG 1 cut(s) 555
SacI GAGCTC 1 cut(s) 944
SatI GCNGC 5 cut(s) 84, 151, 573, 696, 699
Sau3AI GATC 7 cut(s) 40, 228, 288, 519, 645, 654, 921
Sau96I GGNCC 3 cut(s) 547, 752, 1063
SchI GAGTC 2 cut(s) 521, 695
ScrFI CCNGG 2 cut(s) 896, 1061
SduI GDGCHC 2 cut(s) 322, 944
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 297
Sfr274I CTCGAG 2 cut(s) 278, 364
SinI GGWCC 3 cut(s) 547, 752, 1063
SlaI CTCGAG 2 cut(s) 278, 364
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 278, 364
SmoI CTYRAG 2 cut(s) 278, 364
SphI GCATGC 1 cut(s) 220
Sse9I AATT 1 cut(s) 16
SsiI CCGC 6 cut(s) 38, 84, 182, 454, 545, 572
SspMI CTAG 2 cut(s) 599, 767
SstI GAGCTC 1 cut(s) 944
StyD4I CCNGG 2 cut(s) 894, 1059
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 5 cut(s) 91, 406, 746, 778, 1067
TaiI ACGT 2 cut(s) 177, 492
TaqI TCGA 8 cut(s) 222, 279, 291, 342, 365, 470, 525, 530
TaqII GACCGA 1 cut(s) 67
TasI AATT 1 cut(s) 16
TauI GCSGC 2 cut(s) 86, 575
TfiI GAWTC 2 cut(s) 307, 325
TscAI CASTG 1 cut(s) 960
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 150, 695, 698
Tsp45I GTSAC 1 cut(s) 3
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 1 cut(s) 960
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 3 cut(s) 547, 752, 1063
XapI RAATTY 1 cut(s) 16
XceI RCATGY 3 cut(s) 220, 541, 1048
XhoI CTCGAG 2 cut(s) 278, 364
XspI CTAG 2 cut(s) 599, 767
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.