FvH4_3g05180

P21-activated protein kinase-interacting protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015485
Physical Location & Seq
Reverse (-)
2992239 .. 2994530
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05180.t1

Sequence Viewer

Length: 1032 bp
ATGAGTCTAATCGCCGGCTCCTACGAGAAATTCCTATGGGGCTTCAAGCTCAAACCCCTCAACACAGACCCTAATTCCAAAACCCTAACCCTAACCCCTCTCTTCTCCTACCCCTCCCACCTCTCTGCCATCACCTCCCTTGCCGCCGCCGGATCCGCCGCCGCCTCCGGAGGCTCCGACGACACCATCCAGCTCTACGACCTCTCCCACGCCACCTCCGTCGGCTCCCTCAACGACCACGCCGCCACCGTCACCTCCCTCTCCTTCTTCACCCGCCCCGACCTCTCCGTCCCCCTCAACCTCGTCTCCGGCGACGCCGAAGGCTCCTTCTGCATCTATGAGGCCGACCCCTTCGTCCTCCTCAAGAGCCTCAAGCCGCATAGGAAGGCCATAAACGACTTGTCGTTGCACCCCTCCGGGAAGCTGGCTTTGACTGTGGGGAAAGATGAGTGCTTGGCGATGATTAACTTGGTGAGGGGGAGGAGGAGCTTTTATTGCAGGTTGGGGATGGAGGCTAGCTTGGTGGACTTCAATGGTGACGGAGATAAGTTCTATTTGGCTACCGAGGAGAAAGTGACTGTTCATGAGGCTGAGGATGCCAAATTGGTTTGCGAAATGGAGAATCTGAAGCGGGTTCTTTGCGCTGAGCCCGGTGAGAATGGACTTCTGTTTACTGGTGGTGAGGACCGGGGTATTACGGCTTGGGACACAAACACTGGGAAAGTTGCATATCGTATTGAGGATGCACATTCGACCCGTGTGAAAGGGATTGTGGTAATGAAAAGCAGTTCCGGTGATGTAGCCAATGAGGATCCGTATATTGTGGCATCTGGGTCGTCGGATGGGGTTATACGAGTTTGGGATGTTCGCATGGCTGTTAAGGATAACAAGCCAAATCCATTAGCTGAGGCTAATACGAAGTCAAGGCTTACTTGTCTGGCCGGATCAGCTATGAAATCTTTCAAACAGCCACAGGTTGAGAAAAGAGTGAAGAAAAAATTGAAGCATGATGCAGCAAAAGATGGCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022613 GO:0042254 GO:0042273 GO:0044085 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

344

Amino Acids

37.02

Weight (kDa)

6.47

Isoelectric Point (pI)

20.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 36 - 154 2.3e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 40 - 156 3.4e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 44 - 208 8.8e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 49 - 247 1.8e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 49 - 161 7.6e-09 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 54 - 188 9.1e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 124 - 291 1.8e-10 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 127 - 296 4.7e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 172 - 292 1.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 203 - 293 7.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 212 - 291 7.9e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 215 - 292 3.2e-07 Prp19 WD40 domain
WDR55 PF24796 222 - 292 1.6e-07 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65030
fragaria_vesca FvH4_3g05180
malus_domestica MD05G1280700.v1.1 MD10G1259100.v1.1
prunus_persica Prupe.4G084200_v2.0.a1
pyrus_communis pycom10g21560
rosa_chinensis RchiOBHm_Chr5g0014571
rosa_laevigata RLG00000032129
rosa_multiflora Rmu_co8518221.1_g000002 Rmu_sc0000120.1_g000004
rosa_roxburghii Rroxscaffold_1G00061910
rosa_rugosa Rorug05G0019500
rosa_samantha Rh5AG114700 Rh5BG111600 Rh5CG123100 Rh5DG111000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 287, 353
Acc36I ACCTGC 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 9 cut(s) 144, 147, 156, 159, 162, 243, 274, 377, 631
AclWI GGATC 5 cut(s) 147, 160, 806, 819, 952
AcoI YGGCCR 1 cut(s) 939
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 315
AgsI TTSAA 4 cut(s) 46, 532, 964, 1003
AluBI AGCT 7 cut(s) 49, 193, 424, 489, 519, 905, 950
AluI AGCT 7 cut(s) 49, 193, 424, 489, 519, 905, 950
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 5 cut(s) 147, 160, 806, 819, 952
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 3 cut(s) 342, 387, 939
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 959
AspLEI GCGC 1 cut(s) 644
AspS9I GGNCC 1 cut(s) 685
AsuC2I CCSGG 3 cut(s) 418, 651, 689
AsuHPI GGTGA 8 cut(s) 124, 244, 262, 484, 548, 665, 692, 806
AsuNHI GCTAGC 1 cut(s) 515
AvaII GGWCC 1 cut(s) 685
BamHI GGATCC 2 cut(s) 152, 811
BanII GRGCYC 1 cut(s) 651
BbvCI CCTCAGC 2 cut(s) 591, 906
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 5 cut(s) 137, 194, 502, 836, 1016
BceAI ACGGC 1 cut(s) 714
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BcnI CCSGG 3 cut(s) 418, 651, 689
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 7 cut(s) 144, 147, 159, 162, 243, 377, 1014
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 7 cut(s) 145, 148, 160, 163, 244, 378, 1015
Bme1390I CCNGG 3 cut(s) 418, 651, 689
Bme18I GGWCC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 685
BmiI GGNNCC 6 cut(s) 19, 154, 175, 226, 325, 813
BmrFI CCNGG 3 cut(s) 418, 651, 689
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 5 cut(s) 342, 586, 733, 836, 1000
BmtI GCTAGC 1 cut(s) 519
BmuI ACTGGG 1 cut(s) 726
Bpu10I CCTNAGC 2 cut(s) 591, 906
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 347, 356
BpuMI CCSGG 3 cut(s) 418, 651, 689
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 564, 688
BsaWI WCCGGW 2 cut(s) 167, 791
BsaXI ACNNNNNCTCC 6 cut(s) 188, 200, 218, 230, 290, 320
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 679, 721
BseAI TCCGGA 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 564, 688
BseGI GGATG 6 cut(s) 186, 513, 601, 748, 847, 868
BseMII CTCAG 3 cut(s) 582, 636, 897
BseNI ACTGG 2 cut(s) 679, 721
BseRI GAGGAG 4 cut(s) 350, 496, 499, 581
BseXI GCAGC 1 cut(s) 1025
BshFI GGCC 3 cut(s) 344, 389, 941
BsiSI CCGG 9 cut(s) 15, 150, 168, 309, 417, 651, 688, 792, 942
BslFI GGGAC 2 cut(s) 275, 719
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 275, 719
BsnI GGCC 3 cut(s) 344, 389, 941
Bsp1286I GDGCHC 1 cut(s) 651
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 3 cut(s) 152, 811, 944
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 9 cut(s) 144, 147, 156, 159, 162, 243, 274, 377, 631
BspANI GGCC 3 cut(s) 344, 389, 941
BspCNI CTCAG 3 cut(s) 583, 637, 898
BspEI TCCGGA 1 cut(s) 167
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 6 cut(s) 19, 154, 175, 226, 325, 813
BspMI ACCTGC 1 cut(s) 489
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 5 cut(s) 147, 160, 806, 819, 952
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 2 cut(s) 679, 721
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 564, 688
BssMI GATC 3 cut(s) 152, 811, 944
BssNI GRCGYC 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 250, 436, 580
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 315
BstC8I GCNNGC 3 cut(s) 16, 426, 517
BstDEI CTNAG 3 cut(s) 591, 645, 906
BstF5I GGATG 6 cut(s) 186, 513, 601, 748, 847, 868
BstHHI GCGC 1 cut(s) 644
BstKTI GATC 3 cut(s) 155, 814, 947
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 3 cut(s) 152, 811, 944
BstMWI GCNNNNNNNGC 5 cut(s) 155, 330, 495, 596, 947
BstSCI CCNGG 3 cut(s) 416, 649, 687
BstV1I GCAGC 1 cut(s) 1025
BstX2I RGATCY 2 cut(s) 152, 811
BstYI RGATCY 2 cut(s) 152, 811
BsuRI GGCC 3 cut(s) 344, 389, 941
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 6 cut(s) 186, 513, 601, 748, 847, 868
BtsIMutI CAGTG 1 cut(s) 714
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 3 cut(s) 16, 426, 517
CciI TCATGA 1 cut(s) 583
CfoI GCGC 1 cut(s) 644
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 1 cut(s) 685
CseI GACGC 1 cut(s) 323
CviAII CATG 3 cut(s) 584, 871, 1007
DdeI CTNAG 3 cut(s) 591, 645, 906
DpnI GATC 3 cut(s) 154, 813, 946
DpnII GATC 3 cut(s) 152, 811, 944
DrdI GACNNNNNNGTC 2 cut(s) 287, 353
DseDI GACNNNNNNGTC 2 cut(s) 287, 353
EaeI YGGCCR 1 cut(s) 939
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 647
EcoT38I GRGCYC 1 cut(s) 651
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 3 cut(s) 587, 874, 1010
FalI AAGNNNNNCTT 2 cut(s) 916, 948
FaqI GGGAC 2 cut(s) 275, 719
FatI CATG 3 cut(s) 583, 870, 1006
FauI CCCGC 2 cut(s) 281, 624
Fnu4HI GCNGC 7 cut(s) 144, 147, 159, 162, 243, 377, 1014
FokI GGATG 6 cut(s) 173, 520, 608, 755, 854, 875
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 7 cut(s) 144, 147, 159, 162, 243, 377, 1014
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 643
GluI GCNGC 7 cut(s) 144, 147, 159, 162, 243, 377, 1014
HaeIII GGCC 3 cut(s) 344, 389, 941
HapII CCGG 9 cut(s) 15, 150, 168, 309, 417, 651, 688, 792, 942
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 644
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 3 cut(s) 587, 874, 1010
Hin6I GCGC 1 cut(s) 642
HinP1I GCGC 1 cut(s) 642
HinfI GANTC 2 cut(s) 4, 622
HpaII CCGG 9 cut(s) 15, 150, 168, 309, 417, 651, 688, 792, 942
HphI GGTGA 8 cut(s) 124, 244, 262, 484, 548, 665, 692, 806
Hpy166II GTNNAC 2 cut(s) 526, 672
Hpy188I TCNGA 3 cut(s) 178, 627, 841
Hpy188III TCNNGA 3 cut(s) 168, 364, 584
Hpy8I GTNNAC 2 cut(s) 526, 672
Hpy99I CGWCG 4 cut(s) 182, 224, 317, 841
HpyAV CCTTC 5 cut(s) 274, 314, 337, 361, 379
HpyCH4III ACNGT 3 cut(s) 250, 436, 580
HpyCH4V TGCA 6 cut(s) 333, 409, 498, 728, 746, 1013
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 330, 495, 596, 947
HpyF3I CTNAG 3 cut(s) 591, 645, 906
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 3 cut(s) 587, 874, 1010
HspAI GCGC 1 cut(s) 642
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Kzo9I GATC 3 cut(s) 152, 811, 944
LmnI GCTCC 5 cut(s) 23, 179, 230, 329, 486
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 5 cut(s) 342, 586, 733, 836, 1000
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 250, 536, 574
MalI GATC 3 cut(s) 154, 813, 946
MboI GATC 3 cut(s) 152, 811, 944
MboII GAAGA 3 cut(s) 94, 259, 1003
MflI RGATCY 2 cut(s) 152, 811
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 4 cut(s) 29, 73, 602, 998
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 201, 819
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 959
MseI TTAA 2 cut(s) 465, 879
MspI CCGG 9 cut(s) 15, 150, 168, 309, 417, 651, 688, 792, 942
MspR9I CCNGG 3 cut(s) 418, 651, 689
MwoI GCNNNNNNNGC 5 cut(s) 155, 330, 495, 596, 947
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 3 cut(s) 418, 651, 689
NdeII GATC 3 cut(s) 152, 811, 944
NgoMIV GCCGGC 1 cut(s) 14
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 3 cut(s) 587, 874, 1010
NlaIV GGNNCC 6 cut(s) 19, 154, 175, 226, 325, 813
NmuCI GTSAC 3 cut(s) 250, 536, 574
PagI TCATGA 1 cut(s) 583
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 246, 309
PdiI GCCGGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 959
PfeI GAWTC 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 7 cut(s) 145, 148, 160, 163, 244, 378, 1015
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PspN4I GGNNCC 6 cut(s) 19, 154, 175, 226, 325, 813
PspPI GGNCC 1 cut(s) 685
PsuI RGATCY 2 cut(s) 152, 811
SaqAI TTAA 2 cut(s) 465, 879
SatI GCNGC 7 cut(s) 144, 147, 159, 162, 243, 377, 1014
Sau3AI GATC 3 cut(s) 152, 811, 944
Sau96I GGNCC 1 cut(s) 685
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 3 cut(s) 418, 651, 689
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 5 cut(s) 342, 586, 733, 836, 1000
SinI GGWCC 1 cut(s) 685
SmlI CTYRAG 2 cut(s) 362, 371
SmoI CTYRAG 2 cut(s) 362, 371
Sse9I AATT 4 cut(s) 29, 73, 602, 998
SsiI CCGC 9 cut(s) 144, 147, 156, 159, 162, 243, 274, 377, 631
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 416, 649, 687
TaaI ACNGT 3 cut(s) 250, 436, 580
TaqI TCGA 1 cut(s) 752
TasI AATT 4 cut(s) 29, 73, 602, 998
TauI GCSGC 6 cut(s) 146, 149, 161, 164, 245, 379
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 2 cut(s) 465, 879
Tru9I TTAA 2 cut(s) 465, 879
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 3 cut(s) 250, 536, 574
TseI GCWGC 1 cut(s) 1013
Tsp45I GTSAC 3 cut(s) 250, 536, 574
TspDTI ATGAA 3 cut(s) 572, 794, 968
TspGWI ACGGA 4 cut(s) 208, 277, 555, 804
TspRI CASTG 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 685
XapI RAATTY 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 959
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.