MD05G1280700.v1.1

P21-activated protein kinase-interacting protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015485
Physical Location & Seq
Reverse (-)
41472563 .. 41474948
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1280700.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGAGCCTAATCGCGGGTTCCTACGAGAAGTTCATATGGGGCTTCAAGCTCAGACCCCTAAACCCTAGCTCCGATTCCCAATCCCTAACCCTAACCCCTCTCTTCTCCTACCCCTCCCACCTCTCCTCAATCACCACCGTCGCCACCGCCGGCTCAGCCGCTGCCTCCGGCGGCACCGATGACACCATTCACCTCTACGACATGTCCTCCGCCACCTCCCTCGGTTCCCTCCACGACCACTCCGCCACCGTCACCTCCCTCTCCTTCTTCACCCCTCCCCACCTCTCCTTCCCCCTCAACCTCATCTCCGCCGACGCCGACGGGTCCCTCTGCATCTACGAGGCCGACCCTTTCGTCCTCCTCAAGAGCCTCAAGCCGCACCGGAAGGCCATCAACGACGTGTCGTTGCACCCGTCCGGGAAGCTGGCGCTGACGGTGGGGAAAGACGAGTGCTTGGCGATGATTAATTTGGTGAGGGGGAAGAGGAGTTTCTATTGCAGAGTGGGGAAAGAGGCAAGCTTGGTGAGCTATGATTTCTCTGGAGAGAAGTTCTATATGGCTATGGAGGAGAAGGTGATGGTTCATGAGGCTGAAGATGCTAAGTTGCTTTGCGAATTCGAGAATTCGAAGCGAGTTCTTTGTGTTGAGCCCGGGGAGAATGGAGTTTTATTTACTGGTGGTGAAGATCGGAGTATTACAGCGTGGGACACAAACAGTGGGAAGGTTGCATATCGCATTGAGGATGCACATTCTACCCGTGTGAAGGGAATTGTTACGCTTACAAGCAAAGCAGGTGCTGTAGCCAATGAGGATCCCTATCTTGTGGCATCTGCCTCGTCAGATGGGGTTATACGTGTTTGGGATGTTCGCATGGCTGTTAATGAGAAGCCAAATCCATTGGCCGAGGCTAATACAAAATCAAGGCTTACTTGTCTGGCTGGATCATCTCTTAAATCTTTCAAACGGCCACAGGTTGGAAAAAACGTTCAAGAAGAGAAGCATGATGCAGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022613 GO:0042254 GO:0042273 GO:0044085 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

339

Amino Acids

36.56

Weight (kDa)

6.08

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 37 - 153 1.3e-11 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 41 - 154 1.7e-07 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 44 - 162 9.5e-15 Prp19 WD40 domain
Beta-prop_EML PF23409 45 - 293 1.3e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 49 - 203 4.4e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 49 - 247 1.1e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 51 - 153 2.7e-06 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 53 - 187 1.6e-08 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 127 - 291 6.1e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 172 - 292 2.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 189 - 291 4.1e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 191 - 291 4e-08 WDR5 beta-propeller domain
WDR55 PF24796 209 - 291 2.5e-07 WDR55
WD40_Prp19 PF24814 213 - 292 2.4e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65030
fragaria_vesca FvH4_3g05180
malus_domestica MD05G1280700.v1.1 MD10G1259100.v1.1
prunus_persica Prupe.4G084200_v2.0.a1
pyrus_communis pycom10g21560
rosa_chinensis RchiOBHm_Chr5g0014571
rosa_laevigata RLG00000032129
rosa_multiflora Rmu_co8518221.1_g000002 Rmu_sc0000120.1_g000004
rosa_roxburghii Rroxscaffold_1G00061910
rosa_rugosa Rorug05G0019500
rosa_samantha Rh5AG114700 Rh5BG111600 Rh5CG123100 Rh5DG111000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 782
AasI GACNNNNNNGTC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 173
AccB7I CCANNNNNTGG 1 cut(s) 974
AccII CGCG 1 cut(s) 14
AciI CCGC 8 cut(s) 14, 147, 159, 171, 210, 243, 309, 377
AclI AACGTT 1 cut(s) 984
AclWI GGATC 3 cut(s) 806, 819, 949
AcoI YGGCCR 2 cut(s) 900, 965
AcsI RAATTY 2 cut(s) 614, 622
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 315
AfiI CCNNNNNNNGG 3 cut(s) 13, 763, 974
AflIII ACRYGT 3 cut(s) 201, 399, 853
AgsI TTSAA 3 cut(s) 46, 961, 989
AjiI CACGTC 1 cut(s) 400
AloI GAACNNNNNNTCC 2 cut(s) 969, 1001
AluBI AGCT 5 cut(s) 49, 69, 424, 519, 528
AluI AGCT 5 cut(s) 49, 69, 424, 519, 528
AlwI GGATC 3 cut(s) 806, 819, 949
AlwNI CAGNNNCTG 2 cut(s) 161, 797
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 4 cut(s) 342, 387, 900, 965
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 614, 622
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 1 cut(s) 324
AsuC2I CCSGG 3 cut(s) 418, 651, 652
AsuHPI GGTGA 8 cut(s) 124, 182, 244, 262, 484, 535, 586, 692
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 1 cut(s) 324
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 148
BccI CCATC 3 cut(s) 398, 571, 836
BceAI ACGGC 1 cut(s) 980
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BcnI CCSGG 3 cut(s) 418, 651, 652
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 782
BisI GCNGC 4 cut(s) 159, 162, 172, 377
BlpI GCTNAGC 1 cut(s) 154
BlsI GCNGC 4 cut(s) 160, 163, 173, 378
Bme1390I CCNGG 3 cut(s) 418, 651, 652
Bme18I GGWCC 1 cut(s) 324
BmeT110I CYCGRG 1 cut(s) 650
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
BmrFI CCNGG 3 cut(s) 418, 651, 652
BmsI GCATC 5 cut(s) 342, 586, 733, 836, 994
BpmI CTGGAG 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 154
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 347, 356
BpuMI CCSGG 3 cut(s) 418, 651, 652
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 220, 650, 651, 903
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 8 cut(s) 53, 83, 191, 221, 224, 254, 290, 320
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 763, 974
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 679
Bse8I GATNNNNATC 1 cut(s) 816
BseDI CCNNGG 4 cut(s) 220, 650, 651, 903
BseGI GGATG 2 cut(s) 748, 868
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 3 cut(s) 13, 763, 974
BseMII CTCAG 2 cut(s) 64, 168
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 4 cut(s) 115, 350, 499, 581
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 4 cut(s) 344, 389, 902, 967
BshNI GGYRCC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 5 cut(s) 150, 168, 382, 417, 651
BslFI GGGAC 2 cut(s) 310, 719
BslI CCNNNNNNNGG 3 cut(s) 13, 763, 974
BsmFI GGGAC 2 cut(s) 310, 719
BsnI GGCC 4 cut(s) 344, 389, 902, 967
BsoBI CYCGRG 1 cut(s) 650
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 3 cut(s) 685, 811, 941
Bsp1720I GCTNAGC 1 cut(s) 154
BspACI CCGC 8 cut(s) 14, 147, 159, 171, 210, 243, 309, 377
BspANI GGCC 4 cut(s) 344, 389, 902, 967
BspCNI CTCAG 2 cut(s) 63, 167
BspFNI CGCG 1 cut(s) 14
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
BspMI ACCTGC 1 cut(s) 782
BspPI GGATC 3 cut(s) 806, 819, 949
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 4 cut(s) 220, 650, 651, 903
BssMI GATC 3 cut(s) 685, 811, 941
BssNI GRCGYC 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 139, 250, 436, 716
Bst6I CTCTTC 3 cut(s) 107, 476, 987
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 151, 426, 517
BstDEI CTNAG 3 cut(s) 50, 154, 600
BstF5I GGATG 2 cut(s) 748, 868
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 3 cut(s) 688, 814, 944
BstMBI GATC 3 cut(s) 685, 811, 941
BstMWI GCNNNNNNNGC 3 cut(s) 155, 525, 596
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 3 cut(s) 416, 649, 650
BstSFI CTRYAG 1 cut(s) 798
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 4 cut(s) 344, 389, 902, 967
BtgZI GCGATG 1 cut(s) 473
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 748, 868
BtsIMutI CAGTG 1 cut(s) 721
BveI ACCTGC 1 cut(s) 782
Cac8I GCNNGC 3 cut(s) 151, 426, 517
CaiI CAGNNNCTG 2 cut(s) 161, 797
CciI TCATGA 1 cut(s) 583
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 324
Cfr9I CCCGGG 1 cut(s) 650
CseI GACGC 1 cut(s) 323
CviAII CATG 4 cut(s) 202, 584, 871, 1001
DdeI CTNAG 3 cut(s) 50, 154, 600
DpnI GATC 3 cut(s) 687, 813, 943
DpnII GATC 3 cut(s) 685, 811, 941
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 2 cut(s) 900, 965
Eam1104I CTCTTC 3 cut(s) 107, 476, 987
EarI CTCTTC 3 cut(s) 107, 476, 987
EciI GGCGGA 3 cut(s) 199, 232, 298
Eco24I GRGCYC 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 2 cut(s) 614, 622
EcoT38I GRGCYC 1 cut(s) 651
FaeI CATG 4 cut(s) 205, 587, 874, 1004
FalI AAGNNNNNCTT 2 cut(s) 913, 945
FaqI GGGAC 2 cut(s) 310, 719
FatI CATG 4 cut(s) 201, 583, 870, 1000
FauI CCCGC 1 cut(s) 7
FauNDI CATATG 1 cut(s) 35
Fnu4HI GCNGC 4 cut(s) 159, 162, 172, 377
FokI GGATG 2 cut(s) 755, 875
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 159, 162, 172, 377
FspBI CTAG 1 cut(s) 66
GlaI GCGC 1 cut(s) 429
GluI GCNGC 4 cut(s) 159, 162, 172, 377
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 4 cut(s) 344, 389, 902, 967
HapII CCGG 5 cut(s) 150, 168, 382, 417, 651
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 4 cut(s) 205, 587, 874, 1004
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 1 cut(s) 74
HpaII CCGG 5 cut(s) 150, 168, 382, 417, 651
HphI GGTGA 8 cut(s) 124, 182, 244, 262, 484, 535, 586, 692
Hpy188I TCNGA 4 cut(s) 53, 73, 690, 841
Hpy188III TCNNGA 5 cut(s) 364, 540, 584, 619, 989
Hpy99I CGWCG 4 cut(s) 143, 317, 323, 401
HpyAV CCTTC 6 cut(s) 274, 298, 379, 565, 715, 757
HpyCH4III ACNGT 4 cut(s) 139, 250, 436, 716
HpyCH4IV ACGT 3 cut(s) 399, 853, 984
HpyCH4V TGCA 6 cut(s) 333, 409, 498, 728, 746, 1007
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 525, 596
HpyF3I CTNAG 3 cut(s) 50, 154, 600
HpySE526I ACGT 3 cut(s) 399, 853, 984
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 4 cut(s) 205, 587, 874, 1004
HspAI GCGC 1 cut(s) 428
KflI GGGWCCC 1 cut(s) 324
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Kzo9I GATC 3 cut(s) 685, 811, 941
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 5 cut(s) 342, 586, 733, 836, 994
MaeI CTAG 1 cut(s) 66
MaeII ACGT 3 cut(s) 399, 853, 984
MaeIII GTNAC 2 cut(s) 250, 772
MalI GATC 3 cut(s) 687, 813, 943
MboI GATC 3 cut(s) 685, 811, 941
MboII GAAGA 6 cut(s) 94, 259, 493, 605, 695, 1004
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 4 cut(s) 466, 614, 622, 768
MmeI TCCRAC 1 cut(s) 955
MroNI GCCGGC 1 cut(s) 149
MseI TTAA 3 cut(s) 465, 879, 951
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 5 cut(s) 150, 168, 382, 417, 651
MspR9I CCNGG 3 cut(s) 418, 651, 652
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 155, 525, 596
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 3 cut(s) 418, 651, 652
NdeI CATATG 1 cut(s) 35
NdeII GATC 3 cut(s) 685, 811, 941
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 205, 587, 874, 1004
NlaIV GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
NmeAIII GCCGAG 1 cut(s) 928
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 205
NspV TTCGAA 1 cut(s) 626
PagI TCATGA 1 cut(s) 583
PaqCI CACCTGC 1 cut(s) 782
PciI ACATGT 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PdiI GCCGGC 1 cut(s) 151
PfeI GAWTC 1 cut(s) 74
PflMI CCANNNNNTGG 1 cut(s) 974
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 4 cut(s) 160, 163, 173, 378
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 324
PscI ACATGT 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 324
PspN4I GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
PspPI GGNCC 1 cut(s) 324
PspPPI RGGWCCY 1 cut(s) 324
PstNI CAGNNNCTG 2 cut(s) 161, 797
PsuI RGATCY 1 cut(s) 811
SaqAI TTAA 3 cut(s) 465, 879, 951
SatI GCNGC 4 cut(s) 159, 162, 172, 377
Sau3AI GATC 3 cut(s) 685, 811, 941
Sau96I GGNCC 1 cut(s) 324
ScrFI CCNGG 3 cut(s) 418, 651, 652
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 5 cut(s) 342, 586, 733, 836, 994
SfcI CTRYAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 324
SmaI CCCGGG 1 cut(s) 652
SmlI CTYRAG 2 cut(s) 362, 371
SmoI CTYRAG 2 cut(s) 362, 371
Sse9I AATT 4 cut(s) 466, 614, 622, 768
SsiI CCGC 8 cut(s) 14, 147, 159, 171, 210, 243, 309, 377
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 3 cut(s) 416, 649, 650
TaaI ACNGT 4 cut(s) 139, 250, 436, 716
TaiI ACGT 3 cut(s) 402, 856, 987
TaqI TCGA 2 cut(s) 618, 626
TasI AATT 4 cut(s) 466, 614, 622, 768
TauI GCSGC 3 cut(s) 161, 174, 379
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 3 cut(s) 465, 879, 951
Tru9I TTAA 3 cut(s) 465, 879, 951
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 22, 572
TspMI CCCGGG 1 cut(s) 650
TspRI CASTG 1 cut(s) 721
Van91I CCANNNNNTGG 1 cut(s) 974
VpaK11BI GGWCC 1 cut(s) 324
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 2 cut(s) 614, 622
XceI RCATGY 1 cut(s) 205
XmaI CCCGGG 1 cut(s) 650
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.