Rh5DG111000

P21-activated protein kinase-interacting protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015485
Physical Location & Seq
Forward (+)
10115047 .. 10119875
4829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG111000.1

Sequence Viewer

Length: 1071 bp
ATGAGTCTAATCGCCGGCTCCTACGAGAAATTCTTATGGGGCTTCAAGCTCAAACCCCAAAACACCGACCCTAATTCCAAAACCCTAACCCTAATCCCCCTCTTCTCCTACCCCTCCCACCTCTCCGCCATCACCTCCGTCGCCACCGCCGGCCCCGCCGCCGCCTCCGGAGGCTCCGACGACACCATCCAGCTCTACGACCTCTCCCACGCCACCTCCGTCGGCTCCCTCAACGACCACGCCGCCACCGTCACCTCCCTCTCCTTCTTCGCCCCTCCAAACCTCTCCGTCCCCCTCAACCTCGTCTCCGGCGACGCCGACGGCACCTTCTGCATCTACGAGGCCGACCCCTTCGTCCTCCTCAAGAGCCTCAAGCCGCACCGCAAGGCCATAAACGACTTGTCGTTGCACCCCTCCGGGAAGCTAGCTTTGACTGTGGGGAAAGATGAGTGCTTGGCGATGATTAACTTGGTGAGGGGGAGGAGGAGCTTTTATTGCAGGTTGGGGAAGGAGGCTAGCTTGGTGGACTTCAATGGGTCCGGGGATAAGTTCTATTTGGCTACTGAGGAGAAAGTGACTGTTCATGAGGCTGAGGATGCCAAATTGGTTTGCGAAATGGAGAATCCGAAAAGGGTTCTTTGTGCTGAGCCCGGTGAGAATGGACTTCTGTTTACTGGTGGTGAGGATCGGGGTATTACAGCTTGGGACACAAACACTGGGAAGGTTGCATATCGCATTGAGGATGCACATTCAACCCGCGTTAAAGGGATTGTGGTGTTGAAAAGCAGTTCTGGTGATGTAGACAATGAGGATCCGTATCTTGTAGCATCTGGGTCGTCGGATGGGGTTATACGAGTTTGGGATGTTCGTATGGCTGTCAAGGATAATAAGCCAAATCCCTTAGCTGAGGCTAATACAAAATCAAGGCTTACTTGTCTGGCCGGATCGTCTCTTAAATGTAAGATTTGTGCTTGTGTTTCATATGCTTGTTGTCATATTCGCCACATAATATGTTGTTTGTTTCTAGGTTATCTCTCTTTTGTTATATTGTGTGGTAGCTTGCATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022613 GO:0042254 GO:0042273 GO:0044085 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

356

Amino Acids

38.32

Weight (kDa)

6.36

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 39 - 154 4.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 41 - 157 1.1e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 49 - 161 1.4e-09 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 49 - 247 2.3e-14 THOC3 beta-propeller domain
WD40_Prp19 PF24814 50 - 206 3.5e-15 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 54 - 153 8.1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 124 - 291 4.8e-11 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 127 - 297 7.6e-08 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 146 - 292 7.3e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 192 - 291 1.2e-07 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 202 - 293 8e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 215 - 291 6.2e-07 Prp19 WD40 domain
WDR55 PF24796 222 - 292 2.5e-07 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65030
fragaria_vesca FvH4_3g05180
malus_domestica MD05G1280700.v1.1 MD10G1259100.v1.1
prunus_persica Prupe.4G084200_v2.0.a1
pyrus_communis pycom10g21560
rosa_chinensis RchiOBHm_Chr5g0014571
rosa_laevigata RLG00000032129
rosa_multiflora Rmu_co8518221.1_g000002 Rmu_sc0000120.1_g000004
rosa_roxburghii Rroxscaffold_1G00061910
rosa_rugosa Rorug05G0019500
rosa_samantha Rh5AG114700 Rh5BG111600 Rh5CG123100 Rh5DG111000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 801
AccII CGCG 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 9 cut(s) 126, 147, 156, 159, 162, 243, 377, 382, 757
AclWI GGATC 4 cut(s) 693, 806, 819, 952
AcoI YGGCCR 1 cut(s) 939
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 315
AgsI TTSAA 4 cut(s) 46, 532, 753, 781
AluBI AGCT 9 cut(s) 49, 193, 424, 428, 489, 519, 701, 905, 1059
AluI AGCT 9 cut(s) 49, 193, 424, 428, 489, 519, 701, 905, 1059
Alw26I GTCTC 2 cut(s) 310, 954
AlwI GGATC 4 cut(s) 693, 806, 819, 952
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 4 cut(s) 151, 342, 387, 939
ApoI RAATTY 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 152, 537
AsuC2I CCSGG 3 cut(s) 418, 541, 651
AsuHPI GGTGA 6 cut(s) 124, 244, 484, 665, 692, 806
AsuNHI GCTAGC 2 cut(s) 424, 515
AvaII GGWCC 1 cut(s) 537
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 651
BbvCI CCTCAGC 2 cut(s) 591, 906
BccI CCATC 3 cut(s) 137, 194, 836
BceAI ACGGC 1 cut(s) 337
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BcnI CCSGG 3 cut(s) 418, 541, 651
BcoDI GTCTC 2 cut(s) 310, 954
BfaI CTAG 3 cut(s) 425, 516, 1025
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 4 cut(s) 159, 162, 243, 377
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 4 cut(s) 160, 163, 244, 378
Bme1390I CCNGG 3 cut(s) 418, 541, 651
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 2 cut(s) 152, 537
BmiI GGNNCC 7 cut(s) 19, 154, 175, 226, 325, 538, 813
BmrFI CCNGG 3 cut(s) 418, 541, 651
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 4 cut(s) 342, 586, 733, 836
BmtI GCTAGC 2 cut(s) 428, 519
BmuI ACTGGG 1 cut(s) 726
Bpu10I CCTNAGC 3 cut(s) 591, 901, 906
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 347, 356
BpuMI CCSGG 3 cut(s) 418, 541, 651
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 540
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 188, 200, 218, 230, 290, 320
Bse118I RCCGGY 2 cut(s) 14, 149
Bse1I ACTGG 2 cut(s) 679, 721
Bse8I GATNNNNATC 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 5 cut(s) 186, 601, 748, 847, 868
BseJI GATNNNNATC 1 cut(s) 816
BseMII CTCAG 4 cut(s) 555, 582, 636, 897
BseNI ACTGG 2 cut(s) 679, 721
BseRI GAGGAG 4 cut(s) 350, 496, 499, 581
Bsh1236I CGCG 1 cut(s) 759
BshFI GGCC 4 cut(s) 153, 344, 389, 941
BshNI GGYRCC 1 cut(s) 323
BsiSI CCGG 8 cut(s) 15, 150, 168, 309, 417, 540, 651, 942
BslFI GGGAC 2 cut(s) 275, 719
BsmAI GTCTC 2 cut(s) 310, 954
BsmBI CGTCTC 2 cut(s) 310, 954
BsmFI GGGAC 2 cut(s) 275, 719
BsnI GGCC 4 cut(s) 153, 344, 389, 941
Bsp1286I GDGCHC 1 cut(s) 651
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 3 cut(s) 685, 811, 944
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 9 cut(s) 126, 147, 156, 159, 162, 243, 377, 382, 757
BspANI GGCC 4 cut(s) 153, 344, 389, 941
BspCNI CTCAG 4 cut(s) 556, 583, 637, 898
BspEI TCCGGA 1 cut(s) 167
BspFNI CGCG 1 cut(s) 759
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 7 cut(s) 19, 154, 175, 226, 325, 538, 813
BspMI ACCTGC 1 cut(s) 489
BspOI GCTAGC 2 cut(s) 428, 519
BspPI GGATC 4 cut(s) 693, 806, 819, 952
BspT107I GGYRCC 1 cut(s) 323
BsrFI RCCGGY 2 cut(s) 14, 149
BsrI ACTGG 2 cut(s) 679, 721
BssAI RCCGGY 2 cut(s) 14, 149
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 3 cut(s) 685, 811, 944
BssNI GRCGYC 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 250, 436, 580
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 330
BstC8I GCNNGC 5 cut(s) 16, 151, 426, 517, 1061
BstDEI CTNAG 5 cut(s) 564, 591, 645, 901, 906
BstF5I GGATG 5 cut(s) 186, 601, 748, 847, 868
BstFNI CGCG 1 cut(s) 759
BstKTI GATC 3 cut(s) 688, 814, 947
BstMAI GTCTC 2 cut(s) 310, 954
BstMBI GATC 3 cut(s) 685, 811, 944
BstMWI GCNNNNNNNGC 4 cut(s) 155, 330, 495, 596
BstSCI CCNGG 3 cut(s) 416, 539, 649
BstUI CGCG 1 cut(s) 759
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 4 cut(s) 153, 344, 389, 941
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 5 cut(s) 186, 601, 748, 847, 868
BtsIMutI CAGTG 1 cut(s) 714
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 5 cut(s) 16, 151, 426, 517, 1061
CciI TCATGA 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 14, 149
Cfr13I GGNCC 2 cut(s) 152, 537
CseI GACGC 1 cut(s) 323
CviAII CATG 1 cut(s) 584
DdeI CTNAG 5 cut(s) 564, 591, 645, 901, 906
DpnI GATC 3 cut(s) 687, 813, 946
DpnII GATC 3 cut(s) 685, 811, 944
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 939
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 651
Esp3I CGTCTC 2 cut(s) 310, 954
FaeI CATG 1 cut(s) 587
FalI AAGNNNNNCTT 2 cut(s) 916, 948
FaqI GGGAC 2 cut(s) 275, 719
FatI CATG 1 cut(s) 583
FauI CCCGC 2 cut(s) 163, 764
FauNDI CATATG 1 cut(s) 982
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 4 cut(s) 159, 162, 243, 377
FokI GGATG 5 cut(s) 173, 608, 755, 854, 875
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 159, 162, 243, 377
FspBI CTAG 3 cut(s) 425, 516, 1025
GluI GCNGC 4 cut(s) 159, 162, 243, 377
HaeIII GGCC 4 cut(s) 153, 344, 389, 941
HapII CCGG 8 cut(s) 15, 150, 168, 309, 417, 540, 651, 942
HgaI GACGC 1 cut(s) 323
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 1 cut(s) 587
HinfI GANTC 2 cut(s) 4, 622
HpaII CCGG 8 cut(s) 15, 150, 168, 309, 417, 540, 651, 942
HphI GGTGA 6 cut(s) 124, 244, 484, 665, 692, 806
Hpy166II GTNNAC 3 cut(s) 526, 672, 802
Hpy188I TCNGA 3 cut(s) 178, 627, 841
Hpy188III TCNNGA 3 cut(s) 168, 364, 584
Hpy8I GTNNAC 3 cut(s) 526, 672, 802
Hpy99I CGWCG 6 cut(s) 143, 182, 224, 317, 323, 841
HpyAV CCTTC 5 cut(s) 274, 337, 361, 502, 715
HpyCH4III ACNGT 3 cut(s) 250, 436, 580
HpyCH4V TGCA 6 cut(s) 333, 409, 498, 728, 746, 1063
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 330, 495, 596
HpyF3I CTNAG 5 cut(s) 564, 591, 645, 901, 906
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 1 cut(s) 587
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 2 cut(s) 14, 149
KroNI GCCGGC 2 cut(s) 16, 151
Kzo9I GATC 3 cut(s) 685, 811, 944
LmnI GCTCC 4 cut(s) 23, 179, 230, 486
LweI GCATC 4 cut(s) 342, 586, 733, 836
MaeI CTAG 3 cut(s) 425, 516, 1025
MaeIII GTNAC 2 cut(s) 250, 574
MalI GATC 3 cut(s) 687, 813, 946
MboI GATC 3 cut(s) 685, 811, 944
MboII GAAGA 2 cut(s) 94, 259
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 3 cut(s) 29, 73, 602
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 201, 819
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 2 cut(s) 14, 149
MseI TTAA 4 cut(s) 465, 762, 954, 1069
MspI CCGG 8 cut(s) 15, 150, 168, 309, 417, 540, 651, 942
MspR9I CCNGG 3 cut(s) 418, 541, 651
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 4 cut(s) 155, 330, 495, 596
NaeI GCCGGC 2 cut(s) 16, 151
NciI CCSGG 3 cut(s) 418, 541, 651
NdeI CATATG 1 cut(s) 982
NdeII GATC 3 cut(s) 685, 811, 944
NgoMIV GCCGGC 2 cut(s) 14, 149
NheI GCTAGC 2 cut(s) 424, 515
NlaIII CATG 1 cut(s) 587
NlaIV GGNNCC 7 cut(s) 19, 154, 175, 226, 325, 538, 813
NmuCI GTSAC 2 cut(s) 250, 574
PagI TCATGA 1 cut(s) 583
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 246, 309
PdiI GCCGGC 2 cut(s) 16, 151
PfeI GAWTC 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 4 cut(s) 160, 163, 244, 378
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PspN4I GGNNCC 7 cut(s) 19, 154, 175, 226, 325, 538, 813
PspPI GGNCC 2 cut(s) 152, 537
PsuI RGATCY 1 cut(s) 811
SaqAI TTAA 4 cut(s) 465, 762, 954, 1069
SatI GCNGC 4 cut(s) 159, 162, 243, 377
Sau3AI GATC 3 cut(s) 685, 811, 944
Sau96I GGNCC 2 cut(s) 152, 537
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 3 cut(s) 418, 541, 651
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 4 cut(s) 342, 586, 733, 836
SinI GGWCC 1 cut(s) 537
SmlI CTYRAG 2 cut(s) 362, 371
SmoI CTYRAG 2 cut(s) 362, 371
Sse9I AATT 3 cut(s) 29, 73, 602
SsiI CCGC 9 cut(s) 126, 147, 156, 159, 162, 243, 377, 382, 757
SspMI CTAG 3 cut(s) 425, 516, 1025
StyD4I CCNGG 3 cut(s) 416, 539, 649
TaaI ACNGT 3 cut(s) 250, 436, 580
TasI AATT 3 cut(s) 29, 73, 602
TauI GCSGC 4 cut(s) 161, 164, 245, 379
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 4 cut(s) 465, 762, 954, 1069
Tru9I TTAA 4 cut(s) 465, 762, 954, 1069
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 2 cut(s) 250, 574
Tsp45I GTSAC 2 cut(s) 250, 574
TspDTI ATGAA 2 cut(s) 572, 969
TspGWI ACGGA 4 cut(s) 127, 208, 277, 804
TspRI CASTG 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 801
XspI CTAG 3 cut(s) 425, 516, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.