Rorug05G0019500

P21-activated protein kinase-interacting protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015485
Physical Location & Seq
Forward (+)
1307274 .. 1310097
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0019500.1

Sequence Viewer

Length: 1752 bp
ATGGACAAAGGATGGGGTCTAACCCTTGATTCCGACTCCCTCGGCTTCTTCCTTAACAAGCCTCCTCCTTCCGCTGCTCCTCCTCCTAATAAGAGAACAAACCCGTTTGGCGGTGGTGATAGGATGTTTTCCGGTATTGAATTCCCAGTCAAGTCGAGGCCGGCCGATGAGAACCGCGTGGCCGTTGACGAGGTCGACTTCTTCTCTGACAAGAACAAGAACAAGAACAAGAATGATGATCAAGAGTTGAAAGCCGGCAACAATACCAGTGTCAGTGTCAAGAAGGAGAACTGGTCTGGTTTGGACGCTGTAAACACTGGCTTGCACCTTGTTACTGTAAACACCGGAAGTGATCAATCGACGGTGGATGATGGGGTGTCATCTGATCAGGCGGATAATAAAAGAGCAAAGAATCATGAGCTGGCACAGTTGCAAGTTGAGCTCCAGCGTATGAATGCTGAGAATCAGAGACTGAAGGAAATGCTTGGTCAAGTGACCAACAACTACAATGCTTTACAGATGCATCTTGTGGCAGTAATGCAGCAACAGCAGCAAAGCCAAGCCGCGGCTGACCAAACCTCACAGGATCATGATCAGAATATTGAAGGAAAGTCTGAGGAGAAGAGACATGAGATTGGATCATCAGGATCATTGGTGCCTAGGCAGTTTCTGAACTTGGGTCCAAGGGAAGAAACAAAAGCGGATAATGATGTTTCGAATTCTTCATCCGAAGCCAGAACTCGATCAGCTTCACCTCAGAATAATGTTAGTACTAATGGCAAAAGGGTTGGTAGGGAAGAGAGTCCCGAGTCTGAAACACAAGGCTGGGGGCCTAACAAGGTAGCTAAGCACAACTCCCCACCTAAGCCGATTGATCAGGCCACCGAGGCCACCATGAGGAAAGCTCGAGTCTCAGTTCGAGCCAGATCGGAAGCTCCTATGATCACTGATGGATGCCAATGGCGAAAATATGGACAAAAGATGGCGAAAGGAAACCCATGTCCTCGTGCTTATTATCGGTGTACCATGGCCGTTGGCTGTCCAGTTCGTAAACAGGTACAACGTTGCGCAGAAGACAGGACCATTTTGATCACAACTTACGAAGGCAACCACAACCACCCGCTGCCTCCTGCGGCAATGGCCATGGCATCCACCACTACTGCAGCGGCTAGCATGTTACTTTCGGGGTCCATGTCAAGTGCAGACGGGATCATGAACCAGAACAATTTGCTAGCACGTGCCATTCTTCCATGCTCATCAAGTGTGGCAACAATCTCAGCCTCAGCTCCATTCCCTACTGTCACATTAGACCTCACCACCAGTCCACCATTACAGTTCCAAAGACCACAACAAGGCCAGTTCCAGCTGCCTTTCCCAGGACAGCCCCAGAACTTCGGACCATCAGCCTCAAACCCTCAACTGCCCCAAGGTTTTGGACAAGCCCTATACAACCAGTCTAAATTCTCCGGGCTTCAACTCTCGCAGGAACTAATGAACTCGACATTAGCGCACCAAACACAACAACAACAGCAACAACAACAACAAGGTCAACCATCCTCGTTTGCTGACACCGTGAGTGCTGCCACAGCTGCCATCACTGCTGATCCGACCTTCACTGCCGCCCTAGCCGCCGCTATCACCTCCATTATCGGCGGATCGTCTCATCCCAACAACAACAACTCCACAACCTCAAACAATAGCAATGGCGGCAACAATAACAACAACAAAATCGACAGCTTCCCGGGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022613 GO:0042254 GO:0042273 GO:0044085 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

583

Amino Acids

62.89

Weight (kDa)

7.15

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 317 - 374 2.5e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65030
fragaria_vesca FvH4_3g05180
malus_domestica MD05G1280700.v1.1 MD10G1259100.v1.1
prunus_persica Prupe.4G084200_v2.0.a1
pyrus_communis pycom10g21560
rosa_chinensis RchiOBHm_Chr5g0014571
rosa_laevigata RLG00000032129
rosa_multiflora Rmu_co8518221.1_g000002 Rmu_sc0000120.1_g000004
rosa_roxburghii Rroxscaffold_1G00061910
rosa_rugosa Rorug05G0019500
rosa_samantha Rh5AG114700 Rh5BG111600 Rh5CG123100 Rh5DG111000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 177, 566
AclI AACGTT 1 cut(s) 1063
AclWI GGATC 6 cut(s) 594, 646, 655, 1217, 1598, 1663
AcoI YGGCCR 4 cut(s) 162, 180, 1029, 1140
AcsI RAATTY 3 cut(s) 140, 718, 1460
AcuI CTGAAG 1 cut(s) 494
AcvI CACGTG 1 cut(s) 1238
AfaI GTAC 3 cut(s) 772, 1024, 1059
AfiI CCNNNNNNNGG 5 cut(s) 110, 565, 897, 1352, 1376
AgsI TTSAA 4 cut(s) 140, 250, 605, 1475
AjnI CCWGG 1 cut(s) 1375
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 4 cut(s) 463, 619, 916, 1665
AlwI GGATC 6 cut(s) 594, 646, 655, 1217, 1598, 1663
AlwNI CAGNNNCTG 2 cut(s) 472, 670
Ama87I CYCGRG 3 cut(s) 806, 906, 1741
AoxI GGCC 9 cut(s) 158, 162, 180, 830, 879, 888, 1029, 1140, 1354
ApeKI GCWGC 8 cut(s) 74, 541, 550, 1123, 1163, 1366, 1580, 1589
ApoI RAATTY 3 cut(s) 140, 718, 1460
AspA2I CCTAGG 1 cut(s) 659
AspLEI GCGC 2 cut(s) 1070, 1510
AspS9I GGNCC 5 cut(s) 680, 830, 1080, 1188, 1397
AsuC2I CCSGG 3 cut(s) 1468, 1742, 1743
AsuHPI GGTGA 4 cut(s) 128, 744, 1306, 1630
AsuII TTCGAA 1 cut(s) 716
AsuNHI GCTAGC 2 cut(s) 1169, 1231
AvaI CYCGRG 3 cut(s) 806, 906, 1741
AvaII GGWCC 4 cut(s) 680, 1080, 1188, 1397
AvrII CCTAGG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 1142
BanI GGYRCC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 444
BauI CACGAG 1 cut(s) 1005
BbrPI CACGTG 1 cut(s) 1238
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 1 cut(s) 444
BbvCI CCTCAGC 1 cut(s) 1282
BbvI GCAGC 8 cut(s) 61, 553, 562, 1110, 1175, 1353, 1567, 1576
BccI CCATC 7 cut(s) 6, 365, 944, 976, 1408, 1561, 1601
BceAI ACGGC 2 cut(s) 167, 1016
BciT130I CCWGG 1 cut(s) 1377
BclI TGATCA 7 cut(s) 238, 352, 385, 592, 874, 942, 1089
BcnI CCSGG 3 cut(s) 1468, 1742, 1743
BcoDI GTCTC 4 cut(s) 463, 619, 916, 1665
BfaI CTAG 4 cut(s) 660, 1170, 1232, 1625
BfmI CTRYAG 1 cut(s) 1161
BglI GCCNNNNNGGC 1 cut(s) 887
BlnI CCTAGG 1 cut(s) 659
BlpI GCTNAGC 1 cut(s) 846
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 4 cut(s) 1377, 1468, 1742, 1743
Bme18I GGWCC 4 cut(s) 680, 1080, 1188, 1397
BmeT110I CYCGRG 3 cut(s) 806, 906, 1741
BmgT120I GGNCC 5 cut(s) 680, 830, 1080, 1188, 1397
BmiI GGNNCC 4 cut(s) 657, 681, 831, 1189
BmrFI CCNGG 4 cut(s) 1377, 1468, 1742, 1743
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 4 cut(s) 510, 532, 944, 1157
BmtI GCTAGC 2 cut(s) 1173, 1235
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 1080
BplI GAGNNNNNCTC 2 cut(s) 889, 921
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 2 cut(s) 864, 1282
Bpu1102I GCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 716
BpuMI CCSGG 3 cut(s) 1468, 1742, 1743
BsaAI YACGTR 1 cut(s) 1238
BsaBI GATNNNNATC 1 cut(s) 591
BsaWI WCCGGW 2 cut(s) 131, 344
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 1664, 1694
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 565, 897, 1352, 1376
Bse118I RCCGGY 2 cut(s) 160, 254
Bse1I ACTGG 8 cut(s) 146, 267, 296, 322, 1043, 1320, 1357, 1453
Bse3DI GCAATG 2 cut(s) 1143, 1708
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 1 cut(s) 1377
BseGI GGATG 8 cut(s) 17, 129, 373, 725, 959, 1148, 1553, 1663
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 5 cut(s) 110, 565, 897, 1352, 1376
BseMI GCAATG 2 cut(s) 1143, 1708
BseMII CTCAG 6 cut(s) 450, 606, 770, 927, 1290, 1296
BseNI ACTGG 8 cut(s) 146, 267, 296, 322, 1043, 1320, 1357, 1453
BseRI GAGGAG 4 cut(s) 54, 69, 72, 632
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 8 cut(s) 61, 553, 562, 1110, 1175, 1353, 1567, 1576
BseYI CCCAGC 1 cut(s) 825
BsgI GTGCAG 1 cut(s) 1221
Bsh1236I CGCG 2 cut(s) 177, 566
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 9 cut(s) 160, 164, 182, 832, 881, 890, 1031, 1142, 1356
BshNI GGYRCC 1 cut(s) 655
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 3 cut(s) 806, 906, 1741
BsiSI CCGG 6 cut(s) 132, 161, 255, 345, 1467, 1742
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 5 cut(s) 110, 565, 897, 1352, 1376
BsmAI GTCTC 4 cut(s) 463, 619, 916, 1665
BsmBI CGTCTC 1 cut(s) 1665
BsmFI GGGAC 1 cut(s) 789
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 9 cut(s) 160, 164, 182, 832, 881, 890, 1031, 1142, 1356
BsoBI CYCGRG 3 cut(s) 806, 906, 1741
Bsp119I TTCGAA 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 444
Bsp1720I GCTNAGC 1 cut(s) 846
Bsp19I CCATGG 2 cut(s) 1026, 1143
BspANI GGCC 9 cut(s) 160, 164, 182, 832, 881, 890, 1031, 1142, 1356
BspCNI CTCAG 6 cut(s) 451, 607, 769, 926, 1289, 1295
BspFNI CGCG 2 cut(s) 177, 566
BspHI TCATGA 3 cut(s) 415, 589, 1212
BspLI GGNNCC 4 cut(s) 657, 681, 831, 1189
BspMAI CTGCAG 1 cut(s) 1165
BspOI GCTAGC 2 cut(s) 1173, 1235
BspPI GGATC 6 cut(s) 594, 646, 655, 1217, 1598, 1663
BspT104I TTCGAA 1 cut(s) 716
BspT107I GGYRCC 1 cut(s) 655
BsrDI GCAATG 2 cut(s) 1143, 1708
BsrFI RCCGGY 2 cut(s) 160, 254
BsrI ACTGG 8 cut(s) 146, 267, 296, 322, 1043, 1320, 1357, 1453
BssAI RCCGGY 2 cut(s) 160, 254
BssSI CACGAG 1 cut(s) 1005
BssT1I CCWWGG 5 cut(s) 659, 683, 1026, 1143, 1426
Bst2BI CACGAG 1 cut(s) 1005
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 6 cut(s) 337, 364, 429, 1300, 1335, 1573
Bst6I CTCTTC 2 cut(s) 617, 792
BstBAI YACGTR 1 cut(s) 1238
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 6 cut(s) 162, 256, 323, 423, 1171, 1233
BstDEI CTNAG 8 cut(s) 459, 615, 756, 846, 864, 913, 1276, 1282
BstDSI CCRYGG 3 cut(s) 564, 1026, 1143
BstENI CCTNNNNNAGG 1 cut(s) 1374
BstF5I GGATG 8 cut(s) 17, 129, 373, 725, 959, 1148, 1553, 1663
BstFNI CGCG 2 cut(s) 177, 566
BstHHI GCGC 2 cut(s) 1070, 1510
BstMAI GTCTC 4 cut(s) 463, 619, 916, 1665
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 1 cut(s) 1177
BstSCI CCNGG 4 cut(s) 1375, 1466, 1740, 1741
BstSFI CTRYAG 1 cut(s) 1161
BstUI CGCG 2 cut(s) 177, 566
BstV1I GCAGC 8 cut(s) 61, 553, 562, 1110, 1175, 1353, 1567, 1576
BstV2I GAAGAC 1 cut(s) 1080
BstXI CCANNNNNNTGG 1 cut(s) 1433
BstZI CGGCCG 1 cut(s) 162
BsuRI GGCC 9 cut(s) 160, 164, 182, 832, 881, 890, 1031, 1142, 1356
BtgI CCRYGG 3 cut(s) 564, 1026, 1143
BtsCI GGATG 8 cut(s) 17, 129, 373, 725, 959, 1148, 1553, 1663
BtsI GCAGTG 2 cut(s) 1596, 1614
BtsIMutI CAGTG 6 cut(s) 274, 280, 315, 945, 1596, 1614
Cac8I GCNNGC 6 cut(s) 162, 256, 323, 423, 1171, 1233
CaiI CAGNNNCTG 2 cut(s) 472, 670
CciI TCATGA 3 cut(s) 415, 589, 1212
CfoI GCGC 2 cut(s) 1070, 1510
Cfr10I RCCGGY 2 cut(s) 160, 254
Cfr13I GGNCC 5 cut(s) 680, 830, 1080, 1188, 1397
Cfr42I CCGCGG 1 cut(s) 567
Cfr9I CCCGGG 1 cut(s) 1741
CseI GACGC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 771, 1023, 1058
CviQI GTAC 3 cut(s) 771, 1023, 1058
DdeI CTNAG 8 cut(s) 459, 615, 756, 846, 864, 913, 1276, 1282
EaeI YGGCCR 4 cut(s) 162, 180, 1029, 1140
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 617, 792
EarI CTCTTC 2 cut(s) 617, 792
EciI GGCGGA 2 cut(s) 407, 1668
Ecl136II GAGCTC 1 cut(s) 442
EclXI CGGCCG 1 cut(s) 162
Eco130I CCWWGG 5 cut(s) 659, 683, 1026, 1143, 1426
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 4 cut(s) 680, 1080, 1188, 1397
Eco52I CGGCCG 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 494
Eco72I CACGTG 1 cut(s) 1238
Eco88I CYCGRG 3 cut(s) 806, 906, 1741
EcoICRI GAGCTC 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 1374
EcoO109I RGGNCCY 1 cut(s) 830
EcoRI GAATTC 2 cut(s) 140, 718
EcoRII CCWGG 1 cut(s) 1375
EcoT14I CCWWGG 5 cut(s) 659, 683, 1026, 1143, 1426
EcoT22I ATGCAT 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 5 cut(s) 659, 683, 1026, 1143, 1426
Esp3I CGTCTC 1 cut(s) 1665
FaqI GGGAC 1 cut(s) 789
FauI CCCGC 1 cut(s) 1128
FbaI TGATCA 7 cut(s) 238, 352, 385, 592, 874, 942, 1089
FblI GTMKAC 1 cut(s) 195
FokI GGATG 8 cut(s) 24, 136, 380, 712, 966, 1135, 1540, 1650
FriOI GRGCYC 1 cut(s) 444
FseI GGCCGGCC 1 cut(s) 164
FspBI CTAG 4 cut(s) 660, 1170, 1232, 1625
FspI TGCGCA 1 cut(s) 1069
GlaI GCGC 2 cut(s) 1069, 1509
GsaI CCCAGC 1 cut(s) 829
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 9 cut(s) 160, 164, 182, 832, 881, 890, 1031, 1142, 1356
HapII CCGG 6 cut(s) 132, 161, 255, 345, 1467, 1742
HgaI GACGC 1 cut(s) 314
HhaI GCGC 2 cut(s) 1070, 1510
Hin6I GCGC 2 cut(s) 1068, 1508
HinP1I GCGC 2 cut(s) 1068, 1508
HincII GTYRAC 3 cut(s) 187, 196, 1550
HindII GTYRAC 3 cut(s) 187, 196, 1550
HinfI GANTC 7 cut(s) 29, 35, 412, 463, 802, 809, 909
HpaII CCGG 6 cut(s) 132, 161, 255, 345, 1467, 1742
HphI GGTGA 4 cut(s) 128, 744, 1306, 1630
Hpy166II GTNNAC 8 cut(s) 187, 196, 313, 340, 1023, 1052, 1325, 1550
Hpy188III TCNNGA 7 cut(s) 242, 280, 416, 590, 645, 806, 1213
Hpy8I GTNNAC 8 cut(s) 187, 196, 313, 340, 1023, 1052, 1325, 1550
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 6 cut(s) 78, 277, 469, 599, 1097, 1621
HpyCH4III ACNGT 6 cut(s) 337, 364, 429, 1300, 1335, 1573
HpyCH4IV ACGT 2 cut(s) 1063, 1237
HpyCH4V TGCA 6 cut(s) 325, 433, 523, 541, 1163, 1202
HpyF3I CTNAG 8 cut(s) 459, 615, 756, 846, 864, 913, 1276, 1282
HpySE526I ACGT 2 cut(s) 1063, 1237
HspAI GCGC 2 cut(s) 1068, 1508
KroI GCCGGC 2 cut(s) 160, 254
KroNI GCCGGC 2 cut(s) 162, 256
Ksp22I TGATCA 7 cut(s) 238, 352, 385, 592, 874, 942, 1089
KspI CCGCGG 1 cut(s) 567
LmnI GCTCC 4 cut(s) 82, 447, 940, 1291
Lsp1109I GCAGC 8 cut(s) 61, 553, 562, 1110, 1175, 1353, 1567, 1576
LweI GCATC 4 cut(s) 510, 532, 944, 1157
MaeI CTAG 4 cut(s) 660, 1170, 1232, 1625
MaeII ACGT 2 cut(s) 1063, 1237
MaeIII GTNAC 4 cut(s) 331, 493, 1176, 1300
MboII GAAGA 8 cut(s) 40, 193, 634, 701, 714, 809, 1085, 1238
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 1142
MluCI AATT 4 cut(s) 140, 718, 1225, 1460
MluNI TGGCCA 1 cut(s) 1142
MlyI GAGTC 4 cut(s) 29, 811, 818, 918
MmeI TCCRAC 2 cut(s) 57, 1631
Mox20I TGGCCA 1 cut(s) 1142
Mph1103I ATGCAT 1 cut(s) 525
MroNI GCCGGC 2 cut(s) 160, 254
MscI TGGCCA 1 cut(s) 1142
MseI TTAA 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 1142
MspA1I CMGCKG 6 cut(s) 74, 566, 1123, 1166, 1366, 1589
MspI CCGG 6 cut(s) 132, 161, 255, 345, 1467, 1742
MspR9I CCNGG 4 cut(s) 1377, 1468, 1742, 1743
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 1 cut(s) 1377
MvnI CGCG 2 cut(s) 177, 566
NaeI GCCGGC 2 cut(s) 162, 256
NciI CCSGG 3 cut(s) 1468, 1742, 1743
NcoI CCATGG 2 cut(s) 1026, 1143
NgoMIV GCCGGC 2 cut(s) 160, 254
NheI GCTAGC 2 cut(s) 1169, 1231
NlaIV GGNNCC 4 cut(s) 657, 681, 831, 1189
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 493, 1300
NsbI TGCGCA 1 cut(s) 1069
NsiI ATGCAT 1 cut(s) 525
NspI RCATGY 1 cut(s) 1177
NspV TTCGAA 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 906
PagI TCATGA 3 cut(s) 415, 589, 1212
PctI GAATGC 1 cut(s) 460
PdiI GCCGGC 2 cut(s) 162, 256
PfeI GAWTC 3 cut(s) 29, 412, 463
PflFI GACNNNGTC 1 cut(s) 191
PleI GAGTC 4 cut(s) 29, 810, 817, 917
PmaCI CACGTG 1 cut(s) 1238
PmlI CACGTG 1 cut(s) 1238
PpsI GAGTC 4 cut(s) 29, 810, 817, 917
Ppu21I YACGTR 1 cut(s) 1238
Psp124BI GAGCTC 1 cut(s) 444
Psp1406I AACGTT 1 cut(s) 1063
Psp6I CCWGG 1 cut(s) 1375
PspCI CACGTG 1 cut(s) 1238
PspFI CCCAGC 1 cut(s) 825
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 4 cut(s) 657, 681, 831, 1189
PspPI GGNCC 5 cut(s) 680, 830, 1080, 1188, 1397
PspXI VCTCGAGB 1 cut(s) 906
PstI CTGCAG 1 cut(s) 1165
PstNI CAGNNNCTG 2 cut(s) 472, 670
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 2 cut(s) 1366, 1589
RigI GGCCGGCC 1 cut(s) 164
RsaI GTAC 3 cut(s) 772, 1024, 1059
RsaNI GTAC 3 cut(s) 771, 1023, 1058
SacI GAGCTC 1 cut(s) 444
SacII CCGCGG 1 cut(s) 567
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 1 cut(s) 54
Sau96I GGNCC 5 cut(s) 680, 830, 1080, 1188, 1397
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 4 cut(s) 29, 811, 818, 918
ScrFI CCNGG 4 cut(s) 1377, 1468, 1742, 1743
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 4 cut(s) 510, 532, 944, 1157
SfcI CTRYAG 1 cut(s) 1161
SfiI GGCCNNNNNGGCC 1 cut(s) 887
Sfr274I CTCGAG 1 cut(s) 906
Sfr303I CCGCGG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 716
SgrBI CCGCGG 1 cut(s) 567
SinI GGWCC 4 cut(s) 680, 1080, 1188, 1397
SlaI CTCGAG 1 cut(s) 906
SmaI CCCGGG 1 cut(s) 1743
SmlI CTYRAG 1 cut(s) 906
SmoI CTYRAG 1 cut(s) 906
Sse9I AATT 4 cut(s) 140, 718, 1225, 1460
SspI AATATT 1 cut(s) 601
SspMI CTAG 4 cut(s) 660, 1170, 1232, 1625
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 4 cut(s) 1375, 1466, 1740, 1741
StyI CCWWGG 5 cut(s) 659, 683, 1026, 1143, 1426
TaaI ACNGT 6 cut(s) 337, 364, 429, 1300, 1335, 1573
TaiI ACGT 2 cut(s) 1066, 1240
TaqI TCGA 9 cut(s) 155, 195, 359, 716, 742, 907, 919, 1499, 1731
TasI AATT 4 cut(s) 140, 718, 1225, 1460
TatI WGTACW 1 cut(s) 770
TauI GCSGC 8 cut(s) 566, 569, 1136, 1169, 1622, 1631, 1634, 1710
TfiI GAWTC 3 cut(s) 29, 412, 463
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 6 cut(s) 274, 280, 322, 952, 1603, 1621
TseFI GTSAC 2 cut(s) 493, 1300
TseI GCWGC 8 cut(s) 74, 541, 550, 1123, 1163, 1366, 1580, 1589
Tsp45I GTSAC 2 cut(s) 493, 1300
TspDTI ATGAA 4 cut(s) 467, 714, 1229, 1508
TspMI CCCGGG 1 cut(s) 1741
TspRI CASTG 6 cut(s) 274, 280, 322, 952, 1603, 1621
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 4 cut(s) 680, 1080, 1188, 1397
XagI CCTNNNNNAGG 1 cut(s) 1374
XapI RAATTY 3 cut(s) 140, 718, 1460
XceI RCATGY 1 cut(s) 1177
XhoI CTCGAG 1 cut(s) 906
XmaI CCCGGG 1 cut(s) 1741
XmaJI CCTAGG 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 4 cut(s) 660, 1170, 1232, 1625
ZrmI AGTACT 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.