FvH4_3g05810

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016781
Physical Location & Seq
Reverse (-)
3391806 .. 3392699
894 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05810.t1

Sequence Viewer

Length: 576 bp
ATGAACCACGCGGTTGATCTCCGGCGAAGTAGCAAGACAGCCCCAGCCGCCAAGCTCCGAGAGATAGAAGTCCTAATAATCTCGGCGCAGGACCTCAAGGACGTGAAGCACTTAACACAGATGAGAGCCTACGCGGTGGTGTACGTGGAGAAGGACGTCCACGTGGCTAGGACGCGCGTGGACGAGCACGGCGGCACCAACCCGACGTGGAACGAGGTGGTGAGGATCAAATTCAGGGAGGAGATGGCGGACAAGGAGCACGTGCTGGCGGCGGTGAATGTGGACATCTACGCTCACGGTCACGTGAGGGAGAAGCCGGTGGGGAGCGCCAGGGTGCTGCTCTGCGACGTTTTGAAGGGCGGCGACGCCAGAGAGCCGGCGGACAATCCGATACAGTGCTTGACGGTGCAAGTGTGGAGGCCGTCCGGGAGACCTCATGGACTGCTCAACTTGTGGATCCCTCCCACCGGGAGGTTCTTGATAAGGAGGGAGTCGTTGTCGTTTAGTGTGAGGGAGGCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGTGAAAGGTGGTGAAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

192

Amino Acids

21.67

Weight (kDa)

5.67

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 22 - 121 4.5e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05810
malus_domestica MD05G1286300.v1.1
prunus_persica Prupe.4G078600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0013681
rosa_laevigata RLG00000032040
rosa_multiflora Rmu_co8348725.1_g000001 Rmu_sc0002573.1_g000004
rosa_roxburghii Rroxscaffold_1G00062920
rosa_rugosa Rorug05G0012200
rosa_samantha Rh5AG106600 Rh5BG102700 Rh5CG114600 Rh5DG101400
rosa_wichuraiana Rw5G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 194
AccII CGCG 4 cut(s) 11, 134, 175, 177
AclWI GGATC 3 cut(s) 233, 451, 464
AcsI RAATTY 1 cut(s) 230
AcvI CACGTG 3 cut(s) 163, 262, 304
AcyI GRCGYC 2 cut(s) 156, 366
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 467, 471
AgsI TTSAA 1 cut(s) 355
AjiI CACGTC 2 cut(s) 103, 207
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw21I GWGCWC 2 cut(s) 189, 261
Alw26I GTCTC 1 cut(s) 424
AlwI GGATC 3 cut(s) 233, 451, 464
AoxI GGCC 1 cut(s) 419
ApeKI GCWGC 1 cut(s) 337
ApoI RAATTY 1 cut(s) 230
AspLEI GCGC 3 cut(s) 88, 177, 329
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 2 cut(s) 427, 469
AsuHPI GGTGA 4 cut(s) 232, 286, 565, 575
AvaII GGWCC 1 cut(s) 91
BamHI GGATCC 1 cut(s) 456
BanI GGYRCC 1 cut(s) 194
BbrPI CACGTG 3 cut(s) 163, 262, 304
Bbv12I GWGCWC 2 cut(s) 189, 261
BbvI GCAGC 1 cut(s) 324
BccI CCATC 1 cut(s) 238
BceAI ACGGC 2 cut(s) 205, 406
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 2 cut(s) 427, 469
BcoDI GTCTC 1 cut(s) 424
BfaI CTAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 330
BisI GCNGC 5 cut(s) 48, 193, 270, 338, 361
BlsI GCNGC 5 cut(s) 49, 194, 271, 339, 362
Bme1390I CCNGG 3 cut(s) 331, 427, 469
Bme18I GGWCC 1 cut(s) 91
BmgBI CACGTC 2 cut(s) 103, 207
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 196, 458
BmrFI CCNGG 3 cut(s) 331, 427, 469
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 427, 469
BsaAI YACGTR 4 cut(s) 145, 163, 262, 304
BsaHI GRCGYC 2 cut(s) 156, 366
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 471
Bse118I RCCGGY 2 cut(s) 316, 376
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 467, 471
BseXI GCAGC 1 cut(s) 324
BseYI CCCAGC 1 cut(s) 43
Bsh1236I CGCG 4 cut(s) 11, 134, 175, 177
BshFI GGCC 1 cut(s) 421
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 2 cut(s) 189, 261
BsiSI CCGG 5 cut(s) 22, 317, 377, 426, 468
BslI CCNNNNNNNGG 2 cut(s) 467, 471
BsmAI GTCTC 1 cut(s) 424
BsnI GGCC 1 cut(s) 421
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 189, 261
Bsp143I GATC 3 cut(s) 16, 225, 456
BspANI GGCC 1 cut(s) 421
BspFNI CGCG 4 cut(s) 11, 134, 175, 177
BspLI GGNNCC 2 cut(s) 196, 458
BspPI GGATC 3 cut(s) 233, 451, 464
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 424
BsrFI RCCGGY 2 cut(s) 316, 376
BssAI RCCGGY 2 cut(s) 316, 376
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 16, 225, 456
BssNI GRCGYC 2 cut(s) 156, 366
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 3 cut(s) 299, 396, 406
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 2 cut(s) 156, 366
BstBAI YACGTR 4 cut(s) 145, 163, 262, 304
BstC8I GCNNGC 2 cut(s) 267, 378
BstFNI CGCG 4 cut(s) 11, 134, 175, 177
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 88, 177, 329
BstKTI GATC 3 cut(s) 19, 228, 459
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 3 cut(s) 16, 225, 456
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 329, 425, 467
BstUI CGCG 4 cut(s) 11, 134, 175, 177
BstV1I GCAGC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BsuRI GGCC 1 cut(s) 421
BtrI CACGTC 2 cut(s) 103, 207
BtsIMutI CAGTG 1 cut(s) 401
Cac8I GCNNGC 2 cut(s) 267, 378
CfoI GCGC 3 cut(s) 88, 177, 329
Cfr10I RCCGGY 2 cut(s) 316, 376
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 2 cut(s) 181, 374
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 437
CviJI RGCY 8 cut(s) 41, 47, 55, 128, 167, 316, 376, 421
CviKI_1 RGCY 8 cut(s) 41, 47, 55, 128, 167, 316, 376, 421
CviQI GTAC 1 cut(s) 142
DpnI GATC 3 cut(s) 18, 227, 458
DpnII GATC 3 cut(s) 16, 225, 456
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 3 cut(s) 263, 395, 533
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 91
Eco72I CACGTG 3 cut(s) 163, 262, 304
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 329
FaeI CATG 1 cut(s) 440
FaiI YATR 1 cut(s) 438
FatI CATG 1 cut(s) 436
Fnu4HI GCNGC 5 cut(s) 48, 193, 270, 338, 361
Fsp4HI GCNGC 5 cut(s) 48, 193, 270, 338, 361
FspBI CTAG 1 cut(s) 168
GlaI GCGC 3 cut(s) 87, 176, 328
GluI GCNGC 5 cut(s) 48, 193, 270, 338, 361
GsaI CCCAGC 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 1 cut(s) 421
HapII CCGG 5 cut(s) 22, 317, 377, 426, 468
HgaI GACGC 2 cut(s) 181, 374
HhaI GCGC 3 cut(s) 88, 177, 329
Hin1I GRCGYC 2 cut(s) 156, 366
Hin1II CATG 1 cut(s) 440
Hin6I GCGC 3 cut(s) 86, 175, 327
HinP1I GCGC 3 cut(s) 86, 175, 327
HinfI GANTC 1 cut(s) 491
HpaII CCGG 5 cut(s) 22, 317, 377, 426, 468
HphI GGTGA 4 cut(s) 232, 286, 565, 575
Hpy166II GTNNAC 4 cut(s) 142, 160, 181, 283
Hpy188I TCNGA 2 cut(s) 59, 390
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 4 cut(s) 142, 160, 181, 283
Hpy99I CGWCG 3 cut(s) 208, 350, 368
HpyAV CCTTC 2 cut(s) 145, 349
HpyCH4III ACNGT 3 cut(s) 299, 396, 406
HpyCH4IV ACGT 8 cut(s) 102, 144, 156, 162, 206, 261, 303, 348
HpyCH4V TGCA 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpySE526I ACGT 8 cut(s) 102, 144, 156, 162, 206, 261, 303, 348
Hsp92I GRCGYC 2 cut(s) 156, 366
Hsp92II CATG 1 cut(s) 440
HspAI GCGC 3 cut(s) 86, 175, 327
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
Kzo9I GATC 3 cut(s) 16, 225, 456
LmnI GCTCC 3 cut(s) 60, 256, 324
Lsp1109I GCAGC 1 cut(s) 324
MaeI CTAG 1 cut(s) 168
MaeII ACGT 8 cut(s) 102, 144, 156, 162, 206, 261, 303, 348
MaeIII GTNAC 1 cut(s) 299
MalI GATC 3 cut(s) 18, 227, 458
MboI GATC 3 cut(s) 16, 225, 456
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 189, 261
MluCI AATT 1 cut(s) 230
MlyI GAGTC 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 376
MseI TTAA 1 cut(s) 113
MspI CCGG 5 cut(s) 22, 317, 377, 426, 468
MspR9I CCNGG 3 cut(s) 331, 427, 469
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 4 cut(s) 11, 134, 175, 177
MwoI GCNNNNNNNGC 1 cut(s) 47
NaeI GCCGGC 1 cut(s) 378
NciI CCSGG 2 cut(s) 427, 469
NdeII GATC 3 cut(s) 16, 225, 456
NgoMIV GCCGGC 1 cut(s) 376
NlaIII CATG 1 cut(s) 440
NlaIV GGNNCC 2 cut(s) 196, 458
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 1 cut(s) 299
PdiI GCCGGC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 5 cut(s) 49, 194, 271, 339, 362
PleI GAGTC 1 cut(s) 499
PmaCI CACGTG 3 cut(s) 163, 262, 304
PmlI CACGTG 3 cut(s) 163, 262, 304
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 4 cut(s) 145, 163, 262, 304
PpuMI RGGWCCY 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 329
PspCI CACGTG 3 cut(s) 163, 262, 304
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 196, 458
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 1 cut(s) 456
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 5 cut(s) 48, 193, 270, 338, 361
Sau3AI GATC 3 cut(s) 16, 225, 456
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 3 cut(s) 331, 427, 469
SduI GDGCHC 2 cut(s) 189, 261
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 230
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 3 cut(s) 329, 425, 467
TaaI ACNGT 3 cut(s) 299, 396, 406
TaiI ACGT 8 cut(s) 105, 147, 159, 165, 209, 264, 306, 351
TasI AATT 1 cut(s) 230
TauI GCSGC 4 cut(s) 50, 195, 272, 363
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 230
XspI CTAG 1 cut(s) 168
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.