RLG00000032040

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016781
Physical Location & Seq
Forward (+)
10381264 .. 10381947
684 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032040

Sequence Viewer

Length: 684 bp
ATGAAGGCCAACTTACCATCTACTATATCACTCGGTTCACACTCTCTCGGTCCTTCTCGAGCTCGTCCTCATTCTGTAACTCTCACTCTCAAAATAATCTCCAGAGCGCTAAAAATGAACCAAGCAGTTGAATTCCGGCGAAGGAGCAAGACGGCGGCAGCCACCAAGCTCCGAGAGATAGAGGTCCTGATAATCTCGGCGCAAGACCTCAAGGACGTGAAGCACGTAACGCACATGAGACCTTACGCGGTGGTGTACGTGGAGAAGGACGTGCACGTAGCGAGGACGCGCGTGGACGAGCACGGCGGCACCAACCCCACGTGGAACGAGGTGGTGAGGGTGAAGTTCAGGGAGGAGATGGCGGACAAGGAGCACGTGCTGGCGGCGGTGAACGTGGACATCTACGCTCACGGGCACGTGAGGGAGAAGCCGGTGGGGAGCGCCAGGGTGCTGTTGTGCGACGTTTTGAAGGGTGGCGACGCCCGAGAGCCGGCGGACAATCCGATACAGTGCTTGACGGTGCAGGTGTGGAGGCCGTCCGGGAGGCCTCAGGGGCTGCTCAACCTGTGGGTCCCGCCCACCGGGAGGTTCTTGATAAGGAGGGAGTCGCTGTCGTTTAGTGAGAGGGAGGCGGCGGAGGAAGAGGAGGAGGTGGTGGCGGTGAAAGGTGATGAAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

228

Amino Acids

25.44

Weight (kDa)

8.54

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 60 - 159 6.8e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05810
malus_domestica MD05G1286300.v1.1
prunus_persica Prupe.4G078600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0013681
rosa_laevigata RLG00000032040
rosa_multiflora Rmu_co8348725.1_g000001 Rmu_sc0002573.1_g000004
rosa_roxburghii Rroxscaffold_1G00062920
rosa_rugosa Rorug05G0012200
rosa_samantha Rh5AG106600 Rh5BG102700 Rh5CG114600 Rh5DG101400
rosa_wichuraiana Rw5G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 3 cut(s) 248, 289, 291
AcsI RAATTY 1 cut(s) 131
AcvI CACGTG 3 cut(s) 321, 376, 418
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 1 cut(s) 257
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 3 cut(s) 490, 581, 585
AgsI TTSAA 2 cut(s) 131, 469
AjiI CACGTC 2 cut(s) 217, 271
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 2 cut(s) 62, 169
AluI AGCT 2 cut(s) 62, 169
Alw21I GWGCWC 4 cut(s) 64, 276, 303, 375
Alw26I GTCTC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 272
AlwNI CAGNNNCTG 1 cut(s) 556
Ama87I CYCGRG 2 cut(s) 57, 483
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 3 cut(s) 6, 533, 545
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 158, 556
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 4 cut(s) 109, 202, 291, 443
AspS9I GGNCC 3 cut(s) 50, 184, 571
AsuC2I CCSGG 2 cut(s) 541, 583
AsuHPI GGTGA 5 cut(s) 346, 352, 400, 673, 680
AvaI CYCGRG 2 cut(s) 57, 483
AvaII GGWCC 3 cut(s) 50, 184, 571
AxyI CCTNAGG 1 cut(s) 549
BaeGI GKGCMC 2 cut(s) 276, 417
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 64
BbrPI CACGTG 3 cut(s) 321, 376, 418
Bbv12I GWGCWC 4 cut(s) 64, 276, 303, 375
BbvI GCAGC 2 cut(s) 170, 543
BccI CCATC 2 cut(s) 25, 352
BceAI ACGGC 3 cut(s) 168, 319, 520
BciT130I CCWGG 1 cut(s) 445
BcnI CCSGG 2 cut(s) 541, 583
BcoDI GTCTC 1 cut(s) 232
BfoI RGCGCY 2 cut(s) 110, 444
BfuAI ACCTGC 1 cut(s) 514
BglI GCCNNNNNGGC 1 cut(s) 553
BisI GCNGC 6 cut(s) 156, 159, 307, 384, 557, 633
BlsI GCNGC 6 cut(s) 157, 160, 308, 385, 558, 634
Bme1390I CCNGG 3 cut(s) 445, 541, 583
Bme18I GGWCC 3 cut(s) 50, 184, 571
BmeT110I CYCGRG 2 cut(s) 57, 483
BmgBI CACGTC 2 cut(s) 217, 271
BmgT120I GGNCC 3 cut(s) 50, 184, 571
BmiI GGNNCC 3 cut(s) 310, 572, 573
BmrFI CCNGG 3 cut(s) 445, 541, 583
BpmI CTGGAG 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 541, 583
BsaAI YACGTR 6 cut(s) 226, 259, 277, 321, 376, 418
BsaHI GRCGYC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 596, 626
Bsc4I CCNNNNNNNGG 3 cut(s) 490, 581, 585
Bse118I RCCGGY 2 cut(s) 430, 490
Bse21I CCTNAGG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 490, 581, 585
BseMII CTCAG 1 cut(s) 563
BseRI GAGGAG 3 cut(s) 368, 659, 662
BseSI GKGCMC 2 cut(s) 276, 417
BseXI GCAGC 2 cut(s) 170, 543
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 3 cut(s) 248, 289, 291
BshFI GGCC 3 cut(s) 8, 535, 547
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 4 cut(s) 64, 276, 303, 375
BsiHKCI CYCGRG 2 cut(s) 57, 483
BsiSI CCGG 5 cut(s) 136, 431, 491, 540, 582
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 3 cut(s) 490, 581, 585
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 3 cut(s) 8, 535, 547
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 2 cut(s) 57, 483
Bsp1286I GDGCHC 5 cut(s) 64, 276, 303, 375, 417
BspANI GGCC 3 cut(s) 8, 535, 547
BspCNI CTCAG 1 cut(s) 562
BspFNI CGCG 3 cut(s) 248, 289, 291
BspLI GGNNCC 3 cut(s) 310, 572, 573
BspMI ACCTGC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 232
BsrFI RCCGGY 2 cut(s) 430, 490
BssAI RCCGGY 2 cut(s) 430, 490
BssECI CCNNGG 1 cut(s) 444
BssNI GRCGYC 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 510, 520
Bst6I CTCTTC 2 cut(s) 636, 669
BstACI GRCGYC 1 cut(s) 480
BstBAI YACGTR 6 cut(s) 226, 259, 277, 321, 376, 418
BstC8I GCNNGC 2 cut(s) 381, 492
BstDEI CTNAG 1 cut(s) 549
BstFNI CGCG 3 cut(s) 248, 289, 291
BstH2I RGCGCY 2 cut(s) 110, 444
BstHHI GCGC 4 cut(s) 109, 202, 291, 443
BstMAI GTCTC 1 cut(s) 232
BstMWI GCNNNNNNNGC 2 cut(s) 229, 553
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 3 cut(s) 443, 539, 581
BstSLI GKGCMC 2 cut(s) 276, 417
BstUI CGCG 3 cut(s) 248, 289, 291
BstV1I GCAGC 2 cut(s) 170, 543
Bsu36I CCTNAGG 1 cut(s) 549
BsuRI GGCC 3 cut(s) 8, 535, 547
BtrI CACGTC 2 cut(s) 217, 271
BtsIMutI CAGTG 1 cut(s) 515
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 381, 492
CaiI CAGNNNCTG 1 cut(s) 556
CfoI GCGC 4 cut(s) 109, 202, 291, 443
Cfr10I RCCGGY 2 cut(s) 430, 490
Cfr13I GGNCC 3 cut(s) 50, 184, 571
CseI GACGC 2 cut(s) 295, 488
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 235
CviJI RGCY 9 cut(s) 8, 62, 161, 169, 430, 490, 535, 547, 556
CviKI_1 RGCY 9 cut(s) 8, 62, 161, 169, 430, 490, 535, 547, 556
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 549
Eam1104I CTCTTC 2 cut(s) 636, 669
EarI CTCTTC 2 cut(s) 636, 669
EciI GGCGGA 3 cut(s) 377, 509, 650
Ecl136II GAGCTC 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 3 cut(s) 50, 184, 571
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 62
Eco72I CACGTG 3 cut(s) 321, 376, 418
Eco81I CCTNAGG 1 cut(s) 549
Eco88I CYCGRG 2 cut(s) 57, 483
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 2 cut(s) 184, 571
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 64
FaeI CATG 1 cut(s) 238
FaiI YATR 2 cut(s) 26, 236
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 557
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 582
Fnu4HI GCNGC 6 cut(s) 156, 159, 307, 384, 557, 633
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 6 cut(s) 156, 159, 307, 384, 557, 633
GlaI GCGC 4 cut(s) 108, 201, 290, 442
GluI GCNGC 6 cut(s) 156, 159, 307, 384, 557, 633
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 2 cut(s) 110, 444
HaeIII GGCC 3 cut(s) 8, 535, 547
HapII CCGG 5 cut(s) 136, 431, 491, 540, 582
HgaI GACGC 2 cut(s) 295, 488
HhaI GCGC 4 cut(s) 109, 202, 291, 443
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 4 cut(s) 107, 200, 289, 441
HinP1I GCGC 4 cut(s) 107, 200, 289, 441
HinfI GANTC 1 cut(s) 605
HpaII CCGG 5 cut(s) 136, 431, 491, 540, 582
HphI GGTGA 5 cut(s) 346, 352, 400, 673, 680
Hpy166II GTNNAC 6 cut(s) 38, 256, 274, 295, 391, 397
Hpy188I TCNGA 2 cut(s) 173, 504
Hpy188III TCNNGA 4 cut(s) 57, 102, 187, 592
Hpy8I GTNNAC 6 cut(s) 38, 256, 274, 295, 391, 397
Hpy99I CGWCG 2 cut(s) 464, 482
HpyAV CCTTC 4 cut(s) 63, 135, 259, 463
HpyCH4III ACNGT 2 cut(s) 510, 520
HpyCH4V TGCA 2 cut(s) 274, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 553
HpyF3I CTNAG 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 4 cut(s) 107, 200, 289, 441
KflI GGGWCCC 1 cut(s) 571
KroI GCCGGC 1 cut(s) 490
KroNI GCCGGC 1 cut(s) 492
LmnI GCTCC 4 cut(s) 144, 174, 370, 438
Lsp1109I GCAGC 2 cut(s) 170, 543
MaeIII GTNAC 2 cut(s) 76, 226
MboII GAAGA 1 cut(s) 653
MhlI GDGCHC 5 cut(s) 64, 276, 303, 375, 417
MluCI AATT 1 cut(s) 131
MlyI GAGTC 1 cut(s) 614
MroNI GCCGGC 1 cut(s) 490
MspI CCGG 5 cut(s) 136, 431, 491, 540, 582
MspR9I CCNGG 3 cut(s) 445, 541, 583
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 3 cut(s) 248, 289, 291
MwoI GCNNNNNNNGC 2 cut(s) 229, 553
NaeI GCCGGC 1 cut(s) 492
NciI CCSGG 2 cut(s) 541, 583
NgoMIV GCCGGC 1 cut(s) 490
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 310, 572, 573
NmeAIII GCCGAG 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 57
PaqCI CACCTGC 1 cut(s) 514
PceI AGGCCT 1 cut(s) 547
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 6 cut(s) 157, 160, 308, 385, 558, 634
PleI GAGTC 1 cut(s) 613
PmaCI CACGTG 3 cut(s) 321, 376, 418
PmlI CACGTG 3 cut(s) 321, 376, 418
PpsI GAGTC 1 cut(s) 613
Ppu21I YACGTR 6 cut(s) 226, 259, 277, 321, 376, 418
PpuMI RGGWCCY 2 cut(s) 184, 571
Psp124BI GAGCTC 1 cut(s) 64
Psp5II RGGWCCY 2 cut(s) 184, 571
Psp6I CCWGG 1 cut(s) 443
PspCI CACGTG 3 cut(s) 321, 376, 418
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 3 cut(s) 310, 572, 573
PspPI GGNCC 3 cut(s) 50, 184, 571
PspPPI RGGWCCY 2 cut(s) 184, 571
PstNI CAGNNNCTG 1 cut(s) 556
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SacI GAGCTC 1 cut(s) 64
SatI GCNGC 6 cut(s) 156, 159, 307, 384, 557, 633
Sau96I GGNCC 3 cut(s) 50, 184, 571
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 3 cut(s) 445, 541, 583
SduI GDGCHC 5 cut(s) 64, 276, 303, 375, 417
Sfr274I CTCGAG 1 cut(s) 57
SinI GGWCC 3 cut(s) 50, 184, 571
SlaI CTCGAG 1 cut(s) 57
SmlI CTYRAG 2 cut(s) 57, 209
SmoI CTYRAG 2 cut(s) 57, 209
Sse9I AATT 1 cut(s) 131
SseBI AGGCCT 1 cut(s) 547
SstI GAGCTC 1 cut(s) 64
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 3 cut(s) 443, 539, 581
TaaI ACNGT 2 cut(s) 510, 520
TaqI TCGA 1 cut(s) 58
TaqII GACCGA 1 cut(s) 38
TasI AATT 1 cut(s) 131
TauI GCSGC 4 cut(s) 158, 309, 386, 635
TscAI CASTG 1 cut(s) 515
TseI GCWGC 2 cut(s) 158, 556
TspDTI ATGAA 2 cut(s) 17, 131
TspRI CASTG 1 cut(s) 515
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 3 cut(s) 50, 184, 571
XapI RAATTY 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.