MD05G1286300.v1.1

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016781
Physical Location & Seq
Reverse (-)
41930355 .. 41930918
564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1286300.v1.1.491

Sequence Viewer

Length: 564 bp
ATGAGTAAGGTGCCGAAATATCTGCGAGCGGTGCCGGCGGCGACGACGAAGTTCCGAGAAATAGAAGTGCTGATAATCTCGGCGCAGGACCTGAAGAACGTGAAGCACGTGACGCAAGTGAGGGCGTACGCGGTGGTGTACGTGGAGAAGGACTACCACGCGGCCAGGACGAGCGTGGACGAGCACGGCGGGACCAACCCCATGTGGAACGAGGTCGTCAAAGTGAAGTTCCGGGAAGACTTGCTGGAGAACGACGTGCTGGCAGCGCTGAACGTTGATATCTACGCGCACGGGCTCGTGAGGGAGAAGCCGGTGGGGAGCGCGCGGGTGCTGCTGTGCGACGTTTTGAAGGGCGGGGACGCACGGGAGCCGGCGGATAATCCGATACAGTGCATGACGGTGCAGGTGTGGAGGCCGTCGGGTAGGCCCCACGGGCTGCTGAACCTGTGGGTGCCGCCGACGGGGAGGTTCTTGATAAGGAGGGAGTCTTTGTCGTTTAGTGTGAGAGAGGAGGCGGAGGAGGACGAGACAGTGGTGGCGGTGAAAGGTGGCGACGAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

188

Amino Acids

20.89

Weight (kDa)

6.23

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 21 - 119 1e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05810
malus_domestica MD05G1286300.v1.1
prunus_persica Prupe.4G078600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0013681
rosa_laevigata RLG00000032040
rosa_multiflora Rmu_co8348725.1_g000001 Rmu_sc0002573.1_g000004
rosa_roxburghii Rroxscaffold_1G00062920
rosa_rugosa Rorug05G0012200
rosa_samantha Rh5AG106600 Rh5BG102700 Rh5CG114600 Rh5DG101400
rosa_wichuraiana Rw5G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccB1I GGYRCC 3 cut(s) 10, 31, 451
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 5 cut(s) 131, 161, 287, 323, 325
AclI AACGTT 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 113
AcvI CACGTG 1 cut(s) 109
AfaI GTAC 2 cut(s) 128, 140
AfeI AGCGCT 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 461
AgsI TTSAA 1 cut(s) 349
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 1 cut(s) 164
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 91
Aor51HI AGCGCT 1 cut(s) 267
AoxI GGCC 3 cut(s) 162, 413, 425
ApeKI GCWGC 3 cut(s) 263, 331, 436
AspLEI GCGC 5 cut(s) 85, 268, 289, 323, 325
AspS9I GGNCC 3 cut(s) 88, 192, 426
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 2 cut(s) 88, 192
BanI GGYRCC 3 cut(s) 10, 31, 451
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 296
BbrPI CACGTG 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 275, 318, 423
BceAI ACGGC 2 cut(s) 202, 400
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 1 cut(s) 166
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 521
BfoI RGCGCY 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 394
BglI GCCNNNNNGGC 1 cut(s) 433
BisI GCNGC 6 cut(s) 39, 162, 264, 332, 437, 455
BlsI GCNGC 6 cut(s) 40, 163, 265, 333, 438, 456
Bme1390I CCNGG 2 cut(s) 166, 233
Bme18I GGWCC 2 cut(s) 88, 192
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 3 cut(s) 88, 192, 426
BmiI GGNNCC 6 cut(s) 12, 33, 193, 369, 428, 453
BmrFI CCNGG 2 cut(s) 166, 233
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 233
BsaAI YACGTR 2 cut(s) 109, 142
BsaJI CCNNGG 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 3 cut(s) 34, 310, 370
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 430
BseLI CCNNNNNNNGG 1 cut(s) 461
BsePI GCGCGC 1 cut(s) 321
BseRI GAGGAG 2 cut(s) 524, 533
BseXI GCAGC 3 cut(s) 275, 318, 423
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 5 cut(s) 131, 161, 287, 323, 325
BshFI GGCC 3 cut(s) 164, 415, 427
BshNI GGYRCC 3 cut(s) 10, 31, 451
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 4 cut(s) 35, 232, 311, 371
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 2 cut(s) 205, 371
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 2 cut(s) 205, 371
BsnI GGCC 3 cut(s) 164, 415, 427
Bsp1286I GDGCHC 2 cut(s) 186, 297
BspANI GGCC 3 cut(s) 164, 415, 427
BspFNI CGCG 5 cut(s) 131, 161, 287, 323, 325
BspLI GGNNCC 6 cut(s) 12, 33, 193, 369, 428, 453
BspMI ACCTGC 1 cut(s) 394
BspT107I GGYRCC 3 cut(s) 10, 31, 451
BsrBI CCGCTC 1 cut(s) 29
BsrFI RCCGGY 3 cut(s) 34, 310, 370
BssAI RCCGGY 3 cut(s) 34, 310, 370
BssECI CCNNGG 1 cut(s) 430
BssHII GCGCGC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 3 cut(s) 390, 400, 532
BstBAI YACGTR 2 cut(s) 109, 142
BstC8I GCNNGC 5 cut(s) 27, 36, 261, 323, 372
BstDSI CCRYGG 1 cut(s) 430
BstFNI CGCG 5 cut(s) 131, 161, 287, 323, 325
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 5 cut(s) 85, 268, 289, 323, 325
BstMAI GTCTC 1 cut(s) 521
BstMWI GCNNNNNNNGC 6 cut(s) 31, 35, 112, 265, 331, 433
BstNI CCWGG 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 164, 231
BstUI CGCG 5 cut(s) 131, 161, 287, 323, 325
BstV1I GCAGC 3 cut(s) 275, 318, 423
BstV2I GAAGAC 1 cut(s) 243
BsuRI GGCC 3 cut(s) 164, 415, 427
BtgI CCRYGG 1 cut(s) 430
BtrI CACGTC 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 395, 537
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 5 cut(s) 27, 36, 261, 323, 372
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 5 cut(s) 85, 268, 289, 323, 325
Cfr10I RCCGGY 3 cut(s) 34, 310, 370
Cfr13I GGNCC 3 cut(s) 88, 192, 426
CseI GACGC 2 cut(s) 121, 368
Csp6I GTAC 2 cut(s) 127, 139
CviAII CATG 2 cut(s) 202, 394
CviJI RGCY 7 cut(s) 164, 295, 310, 370, 415, 427, 436
CviKI_1 RGCY 7 cut(s) 164, 295, 310, 370, 415, 427, 436
CviQI GTAC 2 cut(s) 127, 139
EaeI YGGCCR 1 cut(s) 162
EciI GGCGGA 2 cut(s) 389, 530
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 2 cut(s) 88, 192
Eco47III AGCGCT 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 88, 426
EcoRII CCWGG 1 cut(s) 164
EcoRV GATATC 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 2 cut(s) 205, 397
FaiI YATR 3 cut(s) 203, 395, 562
FaqI GGGAC 2 cut(s) 205, 371
FatI CATG 2 cut(s) 201, 393
FauI CCCGC 3 cut(s) 182, 318, 347
Fnu4HI GCNGC 6 cut(s) 39, 162, 264, 332, 437, 455
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 6 cut(s) 39, 162, 264, 332, 437, 455
GlaI GCGC 5 cut(s) 84, 267, 288, 322, 324
GluI GCNGC 6 cut(s) 39, 162, 264, 332, 437, 455
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 3 cut(s) 164, 415, 427
HapII CCGG 4 cut(s) 35, 232, 311, 371
HgaI GACGC 2 cut(s) 121, 368
HhaI GCGC 5 cut(s) 85, 268, 289, 323, 325
Hin1II CATG 2 cut(s) 205, 397
Hin6I GCGC 5 cut(s) 83, 266, 287, 321, 323
HinP1I GCGC 5 cut(s) 83, 266, 287, 321, 323
HinfI GANTC 1 cut(s) 485
HpaII CCGG 4 cut(s) 35, 232, 311, 371
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 2 cut(s) 139, 178
Hpy188I TCNGA 2 cut(s) 56, 384
Hpy188III TCNNGA 2 cut(s) 298, 472
Hpy8I GTNNAC 2 cut(s) 139, 178
Hpy99I CGWCG 7 cut(s) 46, 49, 257, 344, 421, 463, 557
HpyAV CCTTC 2 cut(s) 142, 343
HpyCH4III ACNGT 3 cut(s) 390, 400, 532
HpyCH4IV ACGT 6 cut(s) 99, 108, 141, 255, 273, 342
HpyCH4V TGCA 2 cut(s) 393, 403
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 35, 112, 265, 331, 433
HpySE526I ACGT 6 cut(s) 99, 108, 141, 255, 273, 342
Hsp92II CATG 2 cut(s) 205, 397
HspAI GCGC 5 cut(s) 83, 266, 287, 321, 323
KroI GCCGGC 2 cut(s) 34, 370
KroNI GCCGGC 2 cut(s) 36, 372
LmnI GCTCC 2 cut(s) 318, 367
Lsp1109I GCAGC 3 cut(s) 275, 318, 423
MaeII ACGT 6 cut(s) 99, 108, 141, 255, 273, 342
MaeIII GTNAC 1 cut(s) 109
MbiI CCGCTC 1 cut(s) 29
MboII GAAGA 2 cut(s) 106, 248
MhlI GDGCHC 2 cut(s) 186, 297
MlyI GAGTC 1 cut(s) 494
MroNI GCCGGC 2 cut(s) 34, 370
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 4 cut(s) 35, 232, 311, 371
MspR9I CCNGG 2 cut(s) 166, 233
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 5 cut(s) 131, 161, 287, 323, 325
MwoI GCNNNNNNNGC 6 cut(s) 31, 35, 112, 265, 331, 433
NaeI GCCGGC 2 cut(s) 36, 372
NciI CCSGG 1 cut(s) 233
NgoMIV GCCGGC 2 cut(s) 34, 370
NlaIII CATG 2 cut(s) 205, 397
NlaIV GGNNCC 6 cut(s) 12, 33, 193, 369, 428, 453
NmeAIII GCCGAG 1 cut(s) 59
NmuCI GTSAC 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 394
PauI GCGCGC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdiI GCCGGC 2 cut(s) 36, 372
Pfl23II CGTACG 1 cut(s) 126
PfoI TCCNGGA 1 cut(s) 231
PkrI GCNGC 6 cut(s) 40, 163, 265, 333, 438, 456
PleI GAGTC 1 cut(s) 493
PmaCI CACGTG 1 cut(s) 109
PmlI CACGTG 1 cut(s) 109
PpsI GAGTC 1 cut(s) 493
Ppu21I YACGTR 2 cut(s) 109, 142
PpuMI RGGWCCY 1 cut(s) 88
Psp1406I AACGTT 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 164
PspCI CACGTG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 164
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 6 cut(s) 12, 33, 193, 369, 428, 453
PspPI GGNCC 3 cut(s) 88, 192, 426
PspPPI RGGWCCY 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 91
PteI GCGCGC 1 cut(s) 321
RsaI GTAC 2 cut(s) 128, 140
RsaNI GTAC 2 cut(s) 127, 139
RseI CAYNNNNRTG 1 cut(s) 398
SatI GCNGC 6 cut(s) 39, 162, 264, 332, 437, 455
Sau96I GGNCC 3 cut(s) 88, 192, 426
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 2 cut(s) 166, 233
SduI GDGCHC 2 cut(s) 186, 297
SinI GGWCC 2 cut(s) 88, 192
SmiMI CAYNNNNRTG 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 164, 231
TaaI ACNGT 3 cut(s) 390, 400, 532
TaiI ACGT 6 cut(s) 102, 111, 144, 258, 276, 345
TauI GCSGC 3 cut(s) 41, 164, 457
TscAI CASTG 2 cut(s) 395, 537
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 263, 331, 436
Tsp45I GTSAC 1 cut(s) 109
TspRI CASTG 2 cut(s) 395, 537
VpaK11BI GGWCC 2 cut(s) 88, 192
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.