Rh5DG101400

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016781
Physical Location & Seq
Forward (+)
9257287 .. 9258158
872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG101400.1

Sequence Viewer

Length: 534 bp
ATGAACCAAGTAGTTGAATTCCGGCGAAGGAGCAAGACGGCGGCGGCCGCCAAGCTCCGAGAGATAGAGGTCCTGATAATCTCGGCGCAAGACCTCAAGGACGTGAAGCACGTAACGCACATGAGACCTTACGCGGTGGTGTACGTGGAGAAGGACGTGCACGTAGCGAGGACGCGCGTGGACGAGCACGGCGGCACCAACCCCACGTGGAACGAGGTGGTGAGGGTGAAGTTCAGGGAGGAGATGGCGGACAAGGAGCACGTGCTGGCGGCGGTGAACGTGGACATCTATGCTCACGGGCACGTGAGGGAGAAGCCGGTGGGGAGCGCCAGGGTGCTGTTGTGCGACGTTTTGAAGGGCGGCGACGCCAGAGAGCCGGCGGACAATCCGATACAGTGCTTGACGGTGCAGGTGTGGAGGCCGTCCGGGAGGCCTCAGGGGCTGCTCAACCTGTGGGTCCCGCCCACCGGGAGGTTCTTGATAAGGAGGGAGTCGCTGTCGTTTAATGATATGCAAGAAACCCATGGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

177

Amino Acids

20.1

Weight (kDa)

8.97

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 22 - 121 3.5e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05810
malus_domestica MD05G1286300.v1.1
prunus_persica Prupe.4G078600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0013681
rosa_laevigata RLG00000032040
rosa_multiflora Rmu_co8348725.1_g000001 Rmu_sc0002573.1_g000004
rosa_roxburghii Rroxscaffold_1G00062920
rosa_rugosa Rorug05G0012200
rosa_samantha Rh5AG106600 Rh5BG102700 Rh5CG114600 Rh5DG101400
rosa_wichuraiana Rw5G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 194
AccII CGCG 3 cut(s) 134, 175, 177
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 17
AcvI CACGTG 3 cut(s) 207, 262, 304
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 467, 471
AgsI TTSAA 2 cut(s) 17, 355
AjiI CACGTC 2 cut(s) 103, 157
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw21I GWGCWC 3 cut(s) 162, 189, 261
Alw26I GTCTC 1 cut(s) 118
Alw44I GTGCAC 1 cut(s) 158
AlwNI CAGNNNCTG 1 cut(s) 442
AoxI GGCC 4 cut(s) 45, 419, 431, 526
ApaLI GTGCAC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 3 cut(s) 88, 177, 329
AspS9I GGNCC 2 cut(s) 70, 457
AsuC2I CCSGG 2 cut(s) 427, 469
AsuHPI GGTGA 3 cut(s) 232, 238, 286
AvaII GGWCC 2 cut(s) 70, 457
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 2 cut(s) 162, 303
BanI GGYRCC 1 cut(s) 194
BbrPI CACGTG 3 cut(s) 207, 262, 304
Bbv12I GWGCWC 3 cut(s) 162, 189, 261
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 238
BceAI ACGGC 3 cut(s) 54, 205, 406
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 2 cut(s) 427, 469
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 439
BisI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
BlsI GCNGC 7 cut(s) 43, 46, 49, 194, 271, 362, 444
Bme1390I CCNGG 3 cut(s) 331, 427, 469
Bme18I GGWCC 2 cut(s) 70, 457
BmgBI CACGTC 2 cut(s) 103, 157
BmgT120I GGNCC 2 cut(s) 70, 457
BmiI GGNNCC 3 cut(s) 196, 458, 459
BmrFI CCNGG 3 cut(s) 331, 427, 469
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 427, 469
BsaAI YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
BsaHI GRCGYC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 330, 523
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 471
Bse118I RCCGGY 2 cut(s) 316, 376
Bse21I CCTNAGG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 2 cut(s) 330, 523
BseLI CCNNNNNNNGG 2 cut(s) 467, 471
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 2 cut(s) 162, 303
BseX3I CGGCCG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 3 cut(s) 134, 175, 177
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 4 cut(s) 47, 421, 433, 528
BshNI GGYRCC 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 3 cut(s) 162, 189, 261
BsiSI CCGG 5 cut(s) 22, 317, 377, 426, 468
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 467, 471
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 4 cut(s) 47, 421, 433, 528
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 4 cut(s) 162, 189, 261, 303
Bsp19I CCATGG 1 cut(s) 523
BspANI GGCC 4 cut(s) 47, 421, 433, 528
BspCNI CTCAG 1 cut(s) 448
BspFNI CGCG 3 cut(s) 134, 175, 177
BspLI GGNNCC 3 cut(s) 196, 458, 459
BspMI ACCTGC 1 cut(s) 400
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 2 cut(s) 316, 376
BssAI RCCGGY 2 cut(s) 316, 376
BssECI CCNNGG 2 cut(s) 330, 523
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 396, 406
BstACI GRCGYC 1 cut(s) 366
BstBAI YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
BstC8I GCNNGC 2 cut(s) 267, 378
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 1 cut(s) 523
BstFNI CGCG 3 cut(s) 134, 175, 177
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 88, 177, 329
BstMAI GTCTC 1 cut(s) 118
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 3 cut(s) 47, 115, 439
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 329, 425, 467
BstSLI GKGCMC 2 cut(s) 162, 303
BstUI CGCG 3 cut(s) 134, 175, 177
BstV1I GCAGC 1 cut(s) 429
BstZI CGGCCG 1 cut(s) 45
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 4 cut(s) 47, 421, 433, 528
BtgI CCRYGG 1 cut(s) 523
BtrI CACGTC 2 cut(s) 103, 157
BtsIMutI CAGTG 1 cut(s) 401
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 267, 378
CaiI CAGNNNCTG 1 cut(s) 442
CciNI GCGGCCGC 1 cut(s) 45
CfoI GCGC 3 cut(s) 88, 177, 329
Cfr10I RCCGGY 2 cut(s) 316, 376
Cfr13I GGNCC 2 cut(s) 70, 457
CseI GACGC 2 cut(s) 181, 374
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 121, 524
CviJI RGCY 8 cut(s) 47, 55, 316, 376, 421, 433, 442, 528
CviKI_1 RGCY 8 cut(s) 47, 55, 316, 376, 421, 433, 442, 528
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 45
EagI CGGCCG 1 cut(s) 45
EciI GGCGGA 2 cut(s) 263, 395
EclXI CGGCCG 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 523
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 70, 457
Eco52I CGGCCG 1 cut(s) 45
Eco72I CACGTG 3 cut(s) 207, 262, 304
Eco81I CCTNAGG 1 cut(s) 435
EcoO109I RGGNCCY 2 cut(s) 70, 457
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 2 cut(s) 124, 527
FaiI YATR 4 cut(s) 122, 291, 512, 525
FaqI GGGAC 1 cut(s) 443
FatI CATG 2 cut(s) 120, 523
FauI CCCGC 1 cut(s) 468
Fnu4HI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
Fsp4HI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
GlaI GCGC 3 cut(s) 87, 176, 328
GluI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 4 cut(s) 47, 421, 433, 528
HapII CCGG 5 cut(s) 22, 317, 377, 426, 468
HgaI GACGC 2 cut(s) 181, 374
HhaI GCGC 3 cut(s) 88, 177, 329
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 124, 527
Hin6I GCGC 3 cut(s) 86, 175, 327
HinP1I GCGC 3 cut(s) 86, 175, 327
HinfI GANTC 1 cut(s) 491
HpaII CCGG 5 cut(s) 22, 317, 377, 426, 468
HphI GGTGA 3 cut(s) 232, 238, 286
Hpy166II GTNNAC 5 cut(s) 142, 160, 181, 277, 283
Hpy188I TCNGA 2 cut(s) 59, 390
Hpy188III TCNNGA 2 cut(s) 73, 478
Hpy8I GTNNAC 5 cut(s) 142, 160, 181, 277, 283
Hpy99I CGWCG 2 cut(s) 350, 368
HpyAV CCTTC 3 cut(s) 21, 145, 349
HpyCH4III ACNGT 2 cut(s) 396, 406
HpyCH4V TGCA 3 cut(s) 160, 409, 514
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 115, 439
HpyF3I CTNAG 1 cut(s) 435
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 124, 527
HspAI GCGC 3 cut(s) 86, 175, 327
KflI GGGWCCC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
LmnI GCTCC 4 cut(s) 30, 60, 256, 324
Lsp1109I GCAGC 1 cut(s) 429
MaeIII GTNAC 1 cut(s) 112
MhlI GDGCHC 4 cut(s) 162, 189, 261, 303
MluCI AATT 1 cut(s) 17
MlyI GAGTC 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 376
MseI TTAA 2 cut(s) 504, 532
MspI CCGG 5 cut(s) 22, 317, 377, 426, 468
MspR9I CCNGG 3 cut(s) 331, 427, 469
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 3 cut(s) 134, 175, 177
MwoI GCNNNNNNNGC 3 cut(s) 47, 115, 439
NaeI GCCGGC 1 cut(s) 378
NciI CCSGG 2 cut(s) 427, 469
NcoI CCATGG 1 cut(s) 523
NgoMIV GCCGGC 1 cut(s) 376
NlaIII CATG 2 cut(s) 124, 527
NlaIV GGNNCC 3 cut(s) 196, 458, 459
NmeAIII GCCGAG 1 cut(s) 62
NotI GCGGCCGC 1 cut(s) 45
PaqCI CACCTGC 1 cut(s) 400
PceI AGGCCT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 7 cut(s) 43, 46, 49, 194, 271, 362, 444
PleI GAGTC 1 cut(s) 499
PmaCI CACGTG 3 cut(s) 207, 262, 304
PmlI CACGTG 3 cut(s) 207, 262, 304
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
PpuMI RGGWCCY 2 cut(s) 70, 457
Psp5II RGGWCCY 2 cut(s) 70, 457
Psp6I CCWGG 1 cut(s) 329
PspCI CACGTG 3 cut(s) 207, 262, 304
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 196, 458, 459
PspPI GGNCC 2 cut(s) 70, 457
PspPPI RGGWCCY 2 cut(s) 70, 457
PstNI CAGNNNCTG 1 cut(s) 442
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 504, 532
SatI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
Sau96I GGNCC 2 cut(s) 70, 457
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 3 cut(s) 331, 427, 469
SduI GDGCHC 4 cut(s) 162, 189, 261, 303
SinI GGWCC 2 cut(s) 70, 457
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 17
SseBI AGGCCT 1 cut(s) 433
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 3 cut(s) 329, 425, 467
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 2 cut(s) 396, 406
TasI AATT 1 cut(s) 17
TauI GCSGC 6 cut(s) 44, 47, 50, 195, 272, 363
Tru1I TTAA 2 cut(s) 504, 532
Tru9I TTAA 2 cut(s) 504, 532
TscAI CASTG 1 cut(s) 401
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 401
VneI GTGCAC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 70, 457
XapI RAATTY 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.