FvH4_3g09350

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012342
Physical Location & Seq
Forward (+)
5462752 .. 5464491
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09350.t1

Sequence Viewer

Length: 1482 bp
ATGGATTCCAAGCTCTCCTTCGTCAAAACCCTAATCCAAAACGCGATCACGAGCAGATCCTACTATACTAGCCGCACCAAGAAGCCTACCCTCTACACGGTCATCAGTCCTCTCGGAGATAAGAAGCAAACCGTAACTCCAGAGCTCGACAACTGGGTCTCCAAAGGCAACAAGGTCAGCGTCGCCGAGCTCCACCGCATCATTCGTGACCTTCGCAAGCGCAAGCGCTTCTCTCAAGCCCTCGAGGTATCTGAGTGGATGAATGAGAAGAGTGTGTACAAGTTTAGGTCGAGTGAGCATGCTGTGCAGCTGGATTTGATAGGCAGGGTTCGGGGGTTTGTTTCGGCAGAAGAGTATTTCACTAAGTTGGAGGATGAAGATAAGGACTGTAGGACATATGGTGCACTTTTGAATTGCTATGTGCGGCAGCTCCAGACAGAGAAGTCTCTTGCTCATTTGCGCAAAATGCAGGAGCTGGGGTTTGCGTCTTCGCCTCTTGATTACAATGGGATCATGTGTTTGTATACGAATAGTGGTCAGCATGAGAAGGTCCCTGGTGTGCTGGCTGAGATGAAGGAGAATAATGTCTCTCCTGACAATTTCAGCTACAGAATCTGCATCAACTCTTATGGTGTGAGGTCGGATCTTGAAGGAGTCGAAAAGGTTTTGAAAGAAATGGAGGGGCAGCCTCACATCGTCATGGACTGGAATACTTATGCTGTTGTTGCTGATTTCTACATGAAGGCGGGCCAAACCAACAAGGCGATTGAAGCCTTAAAGATGTCGGAAGAGAGGTTGGATACGGAAAGTGGGCTTGGCTACAATTTTTTGATTTCACTGTATGCGAATATGGGGAATAAGGATGAAGTTTTGAGGTTATGGGGTCTTGAGAAGAGTGCTTGTAAGAGATGCATAAATAGGGATTATATATGCATGTTGGTGTCTCTGGTGAAGCTGGGTGAGTTTGAAGAGGCTGAGATGGTACTGAAGGAATGGGATATGTCTGGAAACTGTTACGATTTTCGAGTGCCACAGACTGTTGTAGTAGGGTACTCAGCAAAGGGGTTGCATGAGAGAGCAGAAGCTCTGCTTGAAGACTTGATGGAGAAGGGAAAAGCAACCACCCTTAAATGCTGGGAATTAGTAGCTTCTGGATATGTCGCTAAGGGTGAGATGGAAAAAGCTTTCAAGTGCGTGAACGTTGCTCTCAGTGTGTGGACAGAGAAAGGACGGAGACCCAACGATAGTTTGTTCACAACCATACTGAGTTGGCTTGGTGATGAACGCAGTGTTGAAGTTGTAGAAGTTTTTGTTGGATCATTGAGGAAGGTTATGCCAGTGAACAAGCCAATGTATCATGCATTGTTAAAGGCATATATAAGAGGTGGTGAAGAAGTAAGTACTATAGTGGATCGTATGAAGACTGACAAAATAGATCATGACAAAGAAACAGGCAAGATCCTTGACATGAGGCCAACCTAG

Protein Analysis

494

Amino Acids

56.28

Weight (kDa)

7.51

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 168 - 210 2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21705
fragaria_vesca FvH4_3g09350
malus_domestica MD00G1095800.v1.1 MD05G1313800.v1.1
prunus_persica Prupe.4G045900_v2.0.a1
pyrus_communis pycom05g29160 pycom10g24750
rosa_chinensis RchiOBHm_Chr5g0008241
rosa_laevigata RLG00000031552
rosa_multiflora Rmu_co8293593.1_g000001 Rmu_sc0001387.1_g000002 Rmu_sc0001626.1_g000024
rosa_roxburghii Rroxscaffold_1G00068250
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063000 Rh5BG059200 Rh5CG069900 Rh5DG058800
rosa_wichuraiana Rw5G005800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 155, 442
AbsI CCTCGAGG 1 cut(s) 242
Acc16I TGCGCA 1 cut(s) 461
AccI GTMKAC 1 cut(s) 524
AccII CGCG 1 cut(s) 44
AciI CCGC 4 cut(s) 73, 196, 424, 746
AclI AACGTT 1 cut(s) 1200
AclWI GGATC 6 cut(s) 51, 518, 651, 1324, 1419, 1453
AcuI CTGAAG 1 cut(s) 1007
AfaI GTAC 4 cut(s) 278, 984, 1052, 1402
AfeI AGCGCT 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 8 cut(s) 412, 650, 670, 770, 968, 1094, 1189, 1295
AjnI CCWGG 1 cut(s) 553
AjuI GAANNNNNNNTTGG 5 cut(s) 34, 798, 830, 1296, 1328
Alw21I GWGCWC 3 cut(s) 147, 192, 406
Alw26I GTCTC 5 cut(s) 163, 450, 592, 948, 1228
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 6 cut(s) 51, 518, 651, 1324, 1419, 1453
AlwNI CAGNNNCTG 2 cut(s) 475, 615
Ama87I CYCGRG 1 cut(s) 242
Aor51HI AGCGCT 1 cut(s) 227
AoxI GGCC 2 cut(s) 748, 1472
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 307, 427, 685
AspLEI GCGC 3 cut(s) 222, 228, 462
AspS9I GGNCC 2 cut(s) 550, 748
AsuHPI GGTGA 5 cut(s) 961, 971, 1181, 1289, 1400
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 2 cut(s) 147, 192
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BauI CACGAG 1 cut(s) 49
BbsI GAAGAC 3 cut(s) 480, 1101, 1427
Bbv12I GWGCWC 3 cut(s) 147, 192, 406
BbvI GCAGC 3 cut(s) 319, 439, 697
BccI CCATC 3 cut(s) 973, 1096, 1168
BciT130I CCWGG 1 cut(s) 555
BciVI GTATCC 1 cut(s) 793
BcoDI GTCTC 5 cut(s) 163, 450, 592, 948, 1228
BfaI CTAG 2 cut(s) 69, 1480
BfmI CTRYAG 3 cut(s) 388, 607, 1404
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 793
BisI GCNGC 5 cut(s) 73, 308, 425, 428, 686
BlsI GCNGC 5 cut(s) 74, 309, 426, 429, 687
BmcAI AGTACT 1 cut(s) 1402
Bme1390I CCNGG 1 cut(s) 555
Bme18I GGWCC 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 550, 748
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 1 cut(s) 555
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 3 cut(s) 207, 627, 899
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 3 cut(s) 480, 1101, 1427
BpmI CTGGAG 2 cut(s) 123, 416
Bpu10I CCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 2 cut(s) 219, 908
BsaI GGTCTC 2 cut(s) 163, 1228
BsaJI CCNNGG 1 cut(s) 553
BsaXI ACNNNNNCTCC 6 cut(s) 121, 143, 151, 173, 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 3 cut(s) 158, 710, 1337
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 3 cut(s) 264, 379, 868
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMII CTCAG 6 cut(s) 243, 558, 966, 1068, 1222, 1256
BseNI ACTGG 3 cut(s) 158, 710, 1337
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 3 cut(s) 319, 439, 697
BseYI CCCAGC 3 cut(s) 475, 955, 1134
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 750, 1474
BsiHKAI GWGCWC 3 cut(s) 147, 192, 406
BsiHKCI CYCGRG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 5 cut(s) 163, 450, 592, 948, 1228
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 750, 1474
Bso31I GGTCTC 2 cut(s) 163, 1228
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 147, 192, 406
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
BspACI CCGC 4 cut(s) 73, 196, 424, 746
BspANI GGCC 2 cut(s) 750, 1474
BspCNI CTCAG 6 cut(s) 244, 559, 967, 1067, 1221, 1257
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 1 cut(s) 1438
BspLI GGNNCC 1 cut(s) 552
BspPI GGATC 6 cut(s) 51, 518, 651, 1324, 1419, 1453
BspTNI GGTCTC 2 cut(s) 163, 1228
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 3 cut(s) 158, 710, 1337
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
BssNAI GTATAC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 6 cut(s) 100, 133, 389, 840, 1013, 1039
Bst6I CTCTTC 5 cut(s) 263, 345, 783, 887, 963
BstAPI GCANNNNNTGC 1 cut(s) 304
BstAUI TGTACA 1 cut(s) 276
BstC8I GCNNGC 5 cut(s) 218, 224, 300, 564, 748
BstDEI CTNAG 8 cut(s) 252, 363, 567, 975, 1054, 1164, 1208, 1265
BstF5I GGATG 3 cut(s) 264, 379, 868
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 3 cut(s) 222, 228, 462
BstKTI GATC 8 cut(s) 48, 59, 513, 646, 1319, 1414, 1438, 1461
BstMAI GTCTC 5 cut(s) 163, 450, 592, 948, 1228
BstMBI GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
BstMWI GCNNNNNNNGC 4 cut(s) 304, 466, 725, 770
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 2 cut(s) 302, 937
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 3 cut(s) 388, 607, 1404
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 319, 439, 697
BstV2I GAAGAC 3 cut(s) 480, 1101, 1427
BstX2I RGATCY 3 cut(s) 56, 643, 1458
BstYI RGATCY 3 cut(s) 56, 643, 1458
BstZ17I GTATAC 1 cut(s) 525
BsuI GTATCC 1 cut(s) 793
BsuRI GGCC 2 cut(s) 750, 1474
BtsCI GGATG 3 cut(s) 264, 379, 868
BtsI GCAGTG 1 cut(s) 1294
BtsIMutI CAGTG 4 cut(s) 836, 1216, 1294, 1344
Cac8I GCNNGC 5 cut(s) 218, 224, 300, 564, 748
CaiI CAGNNNCTG 2 cut(s) 475, 615
CciI TCATGA 1 cut(s) 1438
CfoI GCGC 3 cut(s) 222, 228, 462
Cfr13I GGNCC 2 cut(s) 550, 748
CseI GACGC 2 cut(s) 169, 474
Csp6I GTAC 4 cut(s) 277, 983, 1051, 1401
CviQI GTAC 4 cut(s) 277, 983, 1051, 1401
DdeI CTNAG 8 cut(s) 252, 363, 567, 975, 1054, 1164, 1208, 1265
DpnI GATC 8 cut(s) 47, 58, 512, 645, 1318, 1413, 1437, 1460
DpnII GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
DrdI GACNNNNNNGTC 2 cut(s) 155, 442
DseDI GACNNNNNNGTC 2 cut(s) 155, 442
Eam1104I CTCTTC 5 cut(s) 263, 345, 783, 887, 963
EarI CTCTTC 5 cut(s) 263, 345, 783, 887, 963
Ecl136II GAGCTC 2 cut(s) 145, 190
Eco24I GRGCYC 2 cut(s) 147, 192
Eco31I GGTCTC 2 cut(s) 163, 1228
Eco47I GGWCC 1 cut(s) 550
Eco47III AGCGCT 1 cut(s) 227
Eco53kI GAGCTC 2 cut(s) 145, 190
Eco57I CTGAAG 1 cut(s) 1007
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 2 cut(s) 145, 190
EcoO109I RGGNCCY 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 553
EcoT22I ATGCAT 3 cut(s) 914, 935, 1363
EcoT38I GRGCYC 2 cut(s) 147, 192
FalI AAGNNNNNCTT 3 cut(s) 34, 1074, 1106
FaqI GGGAC 1 cut(s) 536
FauI CCCGC 1 cut(s) 739
FauNDI CATATG 1 cut(s) 397
FblI GTMKAC 1 cut(s) 524
Fnu4HI GCNGC 5 cut(s) 73, 308, 425, 428, 686
FokI GGATG 3 cut(s) 271, 386, 875
FriOI GRGCYC 2 cut(s) 147, 192
Fsp4HI GCNGC 5 cut(s) 73, 308, 425, 428, 686
FspBI CTAG 2 cut(s) 69, 1480
FspI TGCGCA 1 cut(s) 461
GlaI GCGC 3 cut(s) 221, 227, 461
GluI GCNGC 5 cut(s) 73, 308, 425, 428, 686
GsaI CCCAGC 3 cut(s) 479, 959, 1138
GsuI CTGGAG 2 cut(s) 123, 416
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 750, 1474
HgaI GACGC 2 cut(s) 169, 474
HhaI GCGC 3 cut(s) 222, 228, 462
Hin6I GCGC 3 cut(s) 220, 226, 460
HinP1I GCGC 3 cut(s) 220, 226, 460
HindIII AAGCTT 1 cut(s) 1182
HinfI GANTC 3 cut(s) 5, 612, 654
HphI GGTGA 5 cut(s) 961, 971, 1181, 1289, 1400
Hpy166II GTNNAC 7 cut(s) 277, 404, 525, 1198, 1218, 1254, 1342
Hpy188I TCNGA 4 cut(s) 116, 253, 643, 787
Hpy8I GTNNAC 7 cut(s) 277, 404, 525, 1198, 1218, 1254, 1342
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 9 cut(s) 28, 221, 541, 568, 644, 736, 982, 1102, 1321
HpyCH4III ACNGT 6 cut(s) 100, 133, 389, 840, 1013, 1039
HpyCH4IV ACGT 1 cut(s) 1200
HpyCH4V TGCA 8 cut(s) 307, 404, 469, 618, 912, 933, 1069, 1361
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 466, 725, 770
HpyF3I CTNAG 8 cut(s) 252, 363, 567, 975, 1054, 1164, 1208, 1265
HpySE526I ACGT 1 cut(s) 1200
HspAI GCGC 3 cut(s) 220, 226, 460
Kzo9I GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
LmnI GCTCC 3 cut(s) 195, 435, 472
Lsp1109I GCAGC 3 cut(s) 319, 439, 697
LweI GCATC 3 cut(s) 207, 627, 899
MaeI CTAG 2 cut(s) 69, 1480
MaeII ACGT 1 cut(s) 1200
MaeIII GTNAC 3 cut(s) 133, 206, 1013
MalI GATC 8 cut(s) 47, 58, 512, 645, 1318, 1413, 1437, 1460
MboI GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
MflI RGATCY 3 cut(s) 56, 643, 1458
MhlI GDGCHC 3 cut(s) 147, 192, 406
MluCI AATT 4 cut(s) 412, 598, 823, 1139
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 5 cut(s) 348, 621, 765, 777, 1294
Mph1103I ATGCAT 3 cut(s) 914, 935, 1363
MseI TTAA 3 cut(s) 776, 1128, 1367
MslI CAYNNNNRTG 2 cut(s) 698, 938
MspA1I CMGCKG 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 304, 466, 725, 770
NdeI CATATG 1 cut(s) 397
NdeII GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 206
NsbI TGCGCA 1 cut(s) 461
NsiI ATGCAT 3 cut(s) 914, 935, 1363
NspI RCATGY 2 cut(s) 302, 937
PaeI GCATGC 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 242
PagI TCATGA 1 cut(s) 1438
PfeI GAWTC 2 cut(s) 5, 612
PkrI GCNGC 5 cut(s) 74, 309, 426, 429, 687
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 550
Psp124BI GAGCTC 2 cut(s) 147, 192
Psp1406I AACGTT 1 cut(s) 1200
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 553
PspFI CCCAGC 3 cut(s) 475, 955, 1134
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 2 cut(s) 550, 748
PspPPI RGGWCCY 1 cut(s) 550
PspXI VCTCGAGB 1 cut(s) 242
PstNI CAGNNNCTG 2 cut(s) 475, 615
PsuI RGATCY 3 cut(s) 56, 643, 1458
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 4 cut(s) 278, 984, 1052, 1402
RsaNI GTAC 4 cut(s) 277, 983, 1051, 1401
RseI CAYNNNNRTG 2 cut(s) 698, 938
SacI GAGCTC 2 cut(s) 147, 192
SaqAI TTAA 3 cut(s) 776, 1128, 1367
SatI GCNGC 5 cut(s) 73, 308, 425, 428, 686
Sau3AI GATC 8 cut(s) 45, 56, 510, 643, 1316, 1411, 1435, 1458
Sau96I GGNCC 2 cut(s) 550, 748
ScaI AGTACT 1 cut(s) 1402
SchI GAGTC 1 cut(s) 663
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 3 cut(s) 147, 192, 406
SfaNI GCATC 3 cut(s) 207, 627, 899
SfcI CTRYAG 3 cut(s) 388, 607, 1404
Sfr274I CTCGAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 550
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 698, 938
SmlI CTYRAG 3 cut(s) 234, 242, 887
SmoI CTYRAG 3 cut(s) 234, 242, 887
SphI GCATGC 1 cut(s) 302
Sse9I AATT 4 cut(s) 412, 598, 823, 1139
SsiI CCGC 4 cut(s) 73, 196, 424, 746
SspMI CTAG 2 cut(s) 69, 1480
SstI GAGCTC 2 cut(s) 147, 192
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 6 cut(s) 100, 133, 389, 840, 1013, 1039
TaiI ACGT 1 cut(s) 1203
TaqI TCGA 5 cut(s) 147, 243, 290, 657, 1024
TasI AATT 4 cut(s) 412, 598, 823, 1139
TatI WGTACW 2 cut(s) 276, 1400
TauI GCSGC 2 cut(s) 75, 427
TfiI GAWTC 2 cut(s) 5, 612
Tru1I TTAA 3 cut(s) 776, 1128, 1367
Tru9I TTAA 3 cut(s) 776, 1128, 1367
TscAI CASTG 4 cut(s) 843, 1216, 1294, 1344
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 3 cut(s) 307, 427, 685
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 7 cut(s) 275, 390, 587, 755, 879, 1296, 1433
TspGWI ACGGA 2 cut(s) 818, 1246
TspRI CASTG 4 cut(s) 843, 1216, 1294, 1344
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 550
XceI RCATGY 2 cut(s) 302, 937
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 524
XspI CTAG 2 cut(s) 69, 1480
ZrmI AGTACT 1 cut(s) 1402
Zsp2I ATGCAT 3 cut(s) 914, 935, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.