MD05G1313800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012342
Physical Location & Seq
Forward (+)
44421744 .. 44423438
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1313800.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGTTCCGAAGCTCTTCGCCAGAACCCTAATCCGAAATGCGATGGCGAGCAGGTCCTACTACACGAGCAGGACCAAGAAGCCGACCCTCTACAACAAAATCAGCCCGCTCGGAAACCCTAGCTTGAATGTGGTTCCAGAGCTCGACGACTGGGTCTACAATGGCAACAAAGTCCGAGTCGCCGAGCTTCAGCGCATCATTCACGACCTCCGAAAGCGGAAACGCTTCTCCCAAGCCCTCCAGATTTCAGAGTGGATGAATCAGAAGGGGGTATGCATATTTTCGCCGGTTGAGCATGCCGTGCAGCTGGATTTGATTGGGAAGGTACGCGGGTTTGATTCCGCAGAAGAATATTTCAATAATTTGAGGGACGAAGATAAGAACATCAAGACGTATGGTGCTCTTTTGAATTGCTATGTCCGGCAGCGCCAAGTGGATAAGTCCCTCGCTCATTTGCGCAAAATGAAGGGGATGGGATTTGTTTCCTCGCCTCTTACTTACAACGACATCATGTGTTTGTATACGAATGTTGGCCAGCACGAGAAGGTCCCGGGCGTTCTTGCTGAGATGAAGGAGAACAATGTCCCTCCGGACAACTTCAGCTATAGAATCTGCATCAATTCGTATGGCGTGAGGTCTGATCTTGAAGGAATGGAAAAGGTTTTTAAAGAGATGGAAAGCCAGCCTCGCATTGTCATGGACTGGAACACGTATGCTGTCGTTGCCAATTTCTATGTTAAAGAAGGCCAAACCGATAAGGCAATGAATGCTTTGAAGAAGTCGGAAGAGAGGCTGGATAACAAAGACGGACTTGGCTACAATCATCTGATTTCGCTGTATGCGAGTATGGGAAGTAAGGGTGAAGTTTTGAGGTTATGGGGTGTTGAGAAGAGCGCTTGTAAGAGATGCATAAACAGGGATTATATGGGCATGTTGATGTCTCTGGTGAGGCTCGGTGAGCTCGATGAAGCTGAGAAGGTGATGGAGGAGTGGGAATTGTCTGGAAACTGTTACGATTTTCGGGTGCCACAAACTGTCATTACAGGCTACACCGTAAAGGGATTGTACGAGAGAGCTGAAGCTCTGCTTGAAGACTTGATGGAGAAGGGAAAGGCAACCACAGCGAGAAGTTGGGAAACAGTGGCGGCGGGGTATGTGAATAAGGGTGAGATGAAGAAGGCTTTTAAGTGCTTGAAGGTGGCCCTCCGTCTGTCTTCCGAGAAAAGATGGAAGCCGAACCTCGGAGTAGTGACACTGACAACCTTATTGAGTTGGCTTGGTGACGAAGGCAGTGCTGAAGATGCGGAAGCTTTTGTTGGGGCATTGAGGAAAGTCATCCCGGTGAACAAGCACATGTATCATGCTTTGTTAAAGGCATATGTAAGAGACGGTAAAGAAGTGAATAGTGTTTTGGATCGAATGAAAGCTGATAAAATAGACGGCGATGGCGAAGAGACAGAGAAAATCCTCGCCATGAGAGAATACTAG

Protein Analysis

496

Amino Acids

56.49

Weight (kDa)

8.87

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 166 - 209 5.7e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21705
fragaria_vesca FvH4_3g09350
malus_domestica MD00G1095800.v1.1 MD05G1313800.v1.1
prunus_persica Prupe.4G045900_v2.0.a1
pyrus_communis pycom05g29160 pycom10g24750
rosa_chinensis RchiOBHm_Chr5g0008241
rosa_laevigata RLG00000031552
rosa_multiflora Rmu_co8293593.1_g000001 Rmu_sc0001387.1_g000002 Rmu_sc0001626.1_g000024
rosa_roxburghii Rroxscaffold_1G00068250
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063000 Rh5BG059200 Rh5CG069900 Rh5DG058800
rosa_wichuraiana Rw5G005800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
Acc16I TGCGCA 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 1024
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 2 cut(s) 156, 521
AccII CGCG 1 cut(s) 330
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 7 cut(s) 107, 217, 330, 342, 1145, 1148, 1304
AclWI GGATC 1 cut(s) 1422
AcoI YGGCCR 1 cut(s) 532
AcuI CTGAAG 4 cut(s) 173, 583, 1098, 1317
AfaI GTAC 2 cut(s) 327, 1067
AfeI AGCGCT 1 cut(s) 895
AfiI CCNNNNNNNGG 1 cut(s) 1241
AflIII ACRYGT 2 cut(s) 708, 1353
AgsI TTSAA 7 cut(s) 127, 358, 409, 647, 775, 1091, 1195
AjuI GAANNNNNNNTTGG 4 cut(s) 1299, 1331, 1394, 1426
Alw21I GWGCWC 3 cut(s) 144, 403, 963
Alw26I GTCTC 3 cut(s) 945, 1380, 1448
AlwI GGATC 1 cut(s) 1422
Ama87I CYCGRG 1 cut(s) 550
Aor13HI TCCGGA 1 cut(s) 589
Aor51HI AGCGCT 1 cut(s) 895
AoxI GGCC 3 cut(s) 532, 745, 1200
ApeKI GCWGC 2 cut(s) 304, 424
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
Asp700I GAANNNNTTC 2 cut(s) 14, 224
AspLEI GCGC 4 cut(s) 195, 429, 459, 896
AspS9I GGNCC 4 cut(s) 54, 72, 547, 1201
AsuC2I CCSGG 3 cut(s) 551, 552, 1340
AsuHPI GGTGA 7 cut(s) 872, 958, 968, 991, 1178, 1292, 1354
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 3 cut(s) 54, 72, 547
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BalI TGGCCA 1 cut(s) 534
BanI GGYRCC 1 cut(s) 1024
BanII GRGCYC 2 cut(s) 144, 963
BauI CACGAG 2 cut(s) 64, 539
BbsI GAAGAC 2 cut(s) 1098, 1206
Bbv12I GWGCWC 3 cut(s) 144, 403, 963
BbvI GCAGC 2 cut(s) 316, 436
BccI CCATC 7 cut(s) 37, 466, 667, 976, 1093, 1221, 1439
BceAI ACGGC 2 cut(s) 284, 1456
BcgI CGANNNNNNTGC 2 cut(s) 1274, 1308
BcnI CCSGG 3 cut(s) 551, 552, 1340
BcoDI GTCTC 3 cut(s) 945, 1380, 1448
BfaI CTAG 2 cut(s) 120, 1486
BfmI CTRYAG 1 cut(s) 604
BfoI RGCGCY 2 cut(s) 430, 897
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 305, 425, 1146
BlsI GCNGC 3 cut(s) 306, 426, 1147
Bme1390I CCNGG 3 cut(s) 551, 552, 1340
Bme18I GGWCC 3 cut(s) 54, 72, 547
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 4 cut(s) 54, 72, 547, 1201
BmiI GGNNCC 4 cut(s) 6, 135, 549, 1026
BmrFI CCNGG 3 cut(s) 551, 552, 1340
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 4 cut(s) 204, 624, 896, 1291
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 1098, 1206
BpmI CTGGAG 1 cut(s) 224
BpuMI CCSGG 3 cut(s) 551, 552, 1340
BsaAI YACGTR 1 cut(s) 711
BsaJI CCNNGG 2 cut(s) 550, 1240
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 1 cut(s) 1241
Bse118I RCCGGY 1 cut(s) 286
Bse1I ACTGG 2 cut(s) 155, 707
Bse3DI GCAATG 1 cut(s) 768
BseAI TCCGGA 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 550, 1240
BseGI GGATG 3 cut(s) 261, 477, 1335
BseLI CCNNNNNNNGG 1 cut(s) 1241
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 2 cut(s) 555, 963
BseNI ACTGG 2 cut(s) 155, 707
BseRI GAGGAG 1 cut(s) 1001
BseXI GCAGC 2 cut(s) 316, 436
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 534, 747, 1202
BshNI GGYRCC 1 cut(s) 1024
BsiHKAI GWGCWC 3 cut(s) 144, 403, 963
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 5 cut(s) 287, 421, 551, 590, 1340
BslFI GGGAC 4 cut(s) 383, 427, 533, 569
BslI CCNNNNNNNGG 1 cut(s) 1241
BsmAI GTCTC 3 cut(s) 945, 1380, 1448
BsmBI CGTCTC 1 cut(s) 1380
BsmFI GGGAC 4 cut(s) 383, 427, 533, 569
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 3 cut(s) 534, 747, 1202
BsoBI CYCGRG 1 cut(s) 550
Bsp1286I GDGCHC 3 cut(s) 144, 403, 963
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 2 cut(s) 640, 1414
BspACI CCGC 7 cut(s) 107, 217, 330, 342, 1145, 1148, 1304
BspANI GGCC 3 cut(s) 534, 747, 1202
BspCNI CTCAG 2 cut(s) 556, 964
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 4 cut(s) 6, 135, 549, 1026
BspMI ACCTGC 1 cut(s) 42
BspPI GGATC 1 cut(s) 1422
BspQI GCTCTTC 2 cut(s) 20, 884
BspT107I GGYRCC 1 cut(s) 1024
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 768
BsrFI RCCGGY 1 cut(s) 286
BsrI ACTGG 2 cut(s) 155, 707
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 550, 1240
BssMI GATC 2 cut(s) 640, 1414
BssNAI GTATAC 1 cut(s) 522
BssSI CACGAG 2 cut(s) 64, 539
Bst1107I GTATAC 1 cut(s) 522
Bst2BI CACGAG 2 cut(s) 64, 539
Bst4CI ACNGT 5 cut(s) 1010, 1036, 1054, 1141, 1391
Bst6I CTCTTC 4 cut(s) 20, 780, 884, 1446
BstAPI GCANNNNNTGC 2 cut(s) 301, 767
BstBAI YACGTR 1 cut(s) 711
BstC8I GCNNGC 5 cut(s) 49, 107, 297, 536, 683
BstDEI CTNAG 2 cut(s) 564, 972
BstF5I GGATG 3 cut(s) 261, 477, 1335
BstFNI CGCG 1 cut(s) 330
BstH2I RGCGCY 2 cut(s) 430, 897
BstHHI GCGC 4 cut(s) 195, 429, 459, 896
BstKTI GATC 2 cut(s) 643, 1417
BstMAI GTCTC 3 cut(s) 945, 1380, 1448
BstMBI GATC 2 cut(s) 640, 1414
BstMWI GCNNNNNNNGC 8 cut(s) 292, 301, 687, 722, 767, 958, 1121, 1301
BstNSI RCATGY 3 cut(s) 299, 934, 1357
BstSCI CCNGG 3 cut(s) 549, 550, 1338
BstSFI CTRYAG 1 cut(s) 604
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 316, 436
BstV2I GAAGAC 2 cut(s) 1098, 1206
BstZ17I GTATAC 1 cut(s) 522
BsuRI GGCC 3 cut(s) 534, 747, 1202
BtgZI GCGATG 2 cut(s) 56, 1458
BtsCI GGATG 3 cut(s) 261, 477, 1335
BtsI GCAGTG 1 cut(s) 1297
BtsIMutI CAGTG 3 cut(s) 1146, 1253, 1297
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 5 cut(s) 49, 107, 297, 536, 683
CfoI GCGC 4 cut(s) 195, 429, 459, 896
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 4 cut(s) 54, 72, 547, 1201
Cfr9I CCCGGG 1 cut(s) 550
Csp6I GTAC 2 cut(s) 326, 1066
CviAII CATG 7 cut(s) 296, 511, 697, 931, 1354, 1361, 1474
CviQI GTAC 2 cut(s) 326, 1066
DdeI CTNAG 2 cut(s) 564, 972
DpnI GATC 2 cut(s) 642, 1416
DpnII GATC 2 cut(s) 640, 1414
DraI TTTAAA 1 cut(s) 667
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 4 cut(s) 20, 780, 884, 1446
EarI CTCTTC 4 cut(s) 20, 780, 884, 1446
Ecl136II GAGCTC 2 cut(s) 142, 961
Eco24I GRGCYC 2 cut(s) 144, 963
Eco47I GGWCC 3 cut(s) 54, 72, 547
Eco47III AGCGCT 1 cut(s) 895
Eco53kI GAGCTC 2 cut(s) 142, 961
Eco57I CTGAAG 4 cut(s) 173, 583, 1098, 1317
Eco88I CYCGRG 1 cut(s) 550
EcoICRI GAGCTC 2 cut(s) 142, 961
EcoO109I RGGNCCY 2 cut(s) 54, 547
EcoT22I ATGCAT 2 cut(s) 278, 911
EcoT38I GRGCYC 2 cut(s) 144, 963
Esp3I CGTCTC 1 cut(s) 1380
FaeI CATG 7 cut(s) 299, 514, 700, 934, 1357, 1364, 1477
FalI AAGNNNNNCTT 4 cut(s) 795, 827, 1071, 1103
FaqI GGGAC 4 cut(s) 383, 427, 533, 569
FatI CATG 7 cut(s) 295, 510, 696, 930, 1353, 1360, 1473
FauI CCCGC 3 cut(s) 114, 323, 1141
FauNDI CATATG 1 cut(s) 1378
FblI GTMKAC 2 cut(s) 156, 521
Fnu4HI GCNGC 3 cut(s) 305, 425, 1146
FokI GGATG 3 cut(s) 268, 484, 1322
FriOI GRGCYC 2 cut(s) 144, 963
Fsp4HI GCNGC 3 cut(s) 305, 425, 1146
FspBI CTAG 2 cut(s) 120, 1486
FspI TGCGCA 1 cut(s) 458
GlaI GCGC 4 cut(s) 194, 428, 458, 895
GluI GCNGC 3 cut(s) 305, 425, 1146
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 2 cut(s) 430, 897
HaeIII GGCC 3 cut(s) 534, 747, 1202
HapII CCGG 5 cut(s) 287, 421, 551, 590, 1340
HhaI GCGC 4 cut(s) 195, 429, 459, 896
Hin1II CATG 7 cut(s) 299, 514, 700, 934, 1357, 1364, 1477
Hin6I GCGC 4 cut(s) 193, 427, 457, 894
HinP1I GCGC 4 cut(s) 193, 427, 457, 894
HindIII AAGCTT 1 cut(s) 1308
HinfI GANTC 4 cut(s) 177, 259, 338, 609
HpaII CCGG 5 cut(s) 287, 421, 551, 590, 1340
HphI GGTGA 7 cut(s) 872, 958, 968, 991, 1178, 1292, 1354
Hpy166II GTNNAC 3 cut(s) 157, 522, 1345
Hpy188III TCNNGA 7 cut(s) 137, 203, 241, 388, 590, 644, 1002
Hpy8I GTNNAC 3 cut(s) 157, 522, 1345
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 5 cut(s) 1010, 1036, 1054, 1141, 1391
HpyCH4IV ACGT 2 cut(s) 392, 710
HpyCH4V TGCA 4 cut(s) 276, 304, 615, 909
HpyF10VI GCNNNNNNNGC 8 cut(s) 292, 301, 687, 722, 767, 958, 1121, 1301
HpyF3I CTNAG 2 cut(s) 564, 972
HpySE526I ACGT 2 cut(s) 392, 710
Hsp92II CATG 7 cut(s) 299, 514, 700, 934, 1357, 1364, 1477
HspAI GCGC 4 cut(s) 193, 427, 457, 894
Kpn2I TCCGGA 1 cut(s) 589
Kzo9I GATC 2 cut(s) 640, 1414
LguI GCTCTTC 2 cut(s) 20, 884
Lsp1109I GCAGC 2 cut(s) 316, 436
LweI GCATC 4 cut(s) 204, 624, 896, 1291
MaeI CTAG 2 cut(s) 120, 1486
MaeII ACGT 2 cut(s) 392, 710
MaeIII GTNAC 3 cut(s) 1010, 1249, 1280
MalI GATC 2 cut(s) 642, 1416
MbiI CCGCTC 1 cut(s) 109
MboI GATC 2 cut(s) 640, 1414
MhlI GDGCHC 3 cut(s) 144, 403, 963
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 5 cut(s) 361, 409, 619, 727, 995
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 762
Mox20I TGGCCA 1 cut(s) 534
Mph1103I ATGCAT 2 cut(s) 278, 911
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 14, 224
MscI TGGCCA 1 cut(s) 534
MseI TTAA 4 cut(s) 666, 738, 1185, 1370
MslI CAYNNNNRTG 3 cut(s) 695, 935, 1340
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 5 cut(s) 287, 421, 551, 590, 1340
MspR9I CCNGG 3 cut(s) 551, 552, 1340
Mva1269I GAATGC 1 cut(s) 772
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 8 cut(s) 292, 301, 687, 722, 767, 958, 1121, 1301
NciI CCSGG 3 cut(s) 551, 552, 1340
NdeI CATATG 1 cut(s) 1378
NdeII GATC 2 cut(s) 640, 1414
NlaIII CATG 7 cut(s) 299, 514, 700, 934, 1357, 1364, 1477
NlaIV GGNNCC 4 cut(s) 6, 135, 549, 1026
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 1249, 1280
NsbI TGCGCA 1 cut(s) 458
NsiI ATGCAT 2 cut(s) 278, 911
NspI RCATGY 3 cut(s) 299, 934, 1357
PaeI GCATGC 1 cut(s) 299
PciI ACATGT 1 cut(s) 1353
PciSI GCTCTTC 2 cut(s) 20, 884
PcsI WCGNNNNNNNCGW 3 cut(s) 839, 960, 1446
PctI GAATGC 1 cut(s) 772
PdmI GAANNNNTTC 2 cut(s) 14, 224
PfeI GAWTC 3 cut(s) 259, 338, 609
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 306, 426, 1147
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Ppu21I YACGTR 1 cut(s) 711
PpuMI RGGWCCY 2 cut(s) 54, 547
PscI ACATGT 1 cut(s) 1353
Psp124BI GAGCTC 2 cut(s) 144, 963
Psp5II RGGWCCY 2 cut(s) 54, 547
PspN4I GGNNCC 4 cut(s) 6, 135, 549, 1026
PspPI GGNCC 4 cut(s) 54, 72, 547, 1201
PspPPI RGGWCCY 2 cut(s) 54, 547
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 2 cut(s) 327, 1067
RsaNI GTAC 2 cut(s) 326, 1066
RseI CAYNNNNRTG 3 cut(s) 695, 935, 1340
SacI GAGCTC 2 cut(s) 144, 963
SapI GCTCTTC 2 cut(s) 20, 884
SaqAI TTAA 4 cut(s) 666, 738, 1185, 1370
SatI GCNGC 3 cut(s) 305, 425, 1146
Sau3AI GATC 2 cut(s) 640, 1414
Sau96I GGNCC 4 cut(s) 54, 72, 547, 1201
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 3 cut(s) 551, 552, 1340
SduI GDGCHC 3 cut(s) 144, 403, 963
SfaNI GCATC 4 cut(s) 204, 624, 896, 1291
SfcI CTRYAG 1 cut(s) 604
SinI GGWCC 3 cut(s) 54, 72, 547
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 3 cut(s) 695, 935, 1340
SphI GCATGC 1 cut(s) 299
Sse9I AATT 5 cut(s) 361, 409, 619, 727, 995
SsiI CCGC 7 cut(s) 107, 217, 330, 342, 1145, 1148, 1304
SspI AATATT 1 cut(s) 353
SspMI CTAG 2 cut(s) 120, 1486
SstI GAGCTC 2 cut(s) 144, 963
StyD4I CCNGG 3 cut(s) 549, 550, 1338
TaaI ACNGT 5 cut(s) 1010, 1036, 1054, 1141, 1391
TaiI ACGT 2 cut(s) 395, 713
TaqI TCGA 3 cut(s) 144, 963, 1417
TasI AATT 5 cut(s) 361, 409, 619, 727, 995
TauI GCSGC 1 cut(s) 1148
TfiI GAWTC 3 cut(s) 259, 338, 609
Tru1I TTAA 4 cut(s) 666, 738, 1185, 1370
Tru9I TTAA 4 cut(s) 666, 738, 1185, 1370
TscAI CASTG 3 cut(s) 1146, 1260, 1297
TseFI GTSAC 2 cut(s) 1249, 1280
TseI GCWGC 2 cut(s) 304, 424
Tsp45I GTSAC 2 cut(s) 1249, 1280
TspDTI ATGAA 7 cut(s) 272, 479, 584, 779, 981, 1187, 1436
TspGWI ACGGA 2 cut(s) 822, 1196
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 3 cut(s) 1146, 1260, 1297
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 3 cut(s) 54, 72, 547
XceI RCATGY 3 cut(s) 299, 934, 1357
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 156, 521
XmnI GAANNNNTTC 2 cut(s) 14, 224
XspI CTAG 2 cut(s) 120, 1486
Zsp2I ATGCAT 2 cut(s) 278, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.