FvH4_3g18210

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010471
Physical Location & Seq
Forward (+)
11649717 .. 11656284
6568 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g18210.t5

Sequence Viewer

Length: 1644 bp
ATGAGAGAGCAAGAAACCGAGAAGCTCCGTGCGGTGGTCCGAGACTGCGTCACCAAGCACCTCTACTCGTCGGCGATCTTCTTCGCCGATAAAGTCGCCGCCTTTACCAAAGAGCCCGCCGATATCTACATGCTCGCTCAGGCCCTCTTCCTCGGCCGCCATTACCGTCGCGCCTTCCACCTCCTCCACTCCTCCAAGATCGTCGACCGCGACCTCCGTTTCCGCTACCTCGCCGCCAAGTGCCTCGAAGAGCTGAAGGACTGGCATCAATGCCTTAATATACTCGGTGATGCAAATGTGGATGAACATGGGAACGTATACGACAGAAATGATTCTAATCTCATGTACTTGGATAAAGGCGGTGAAGATCATGAAATCAATATAGCTTCTGCAATATGCTTTCTACGTGGTAAGGCATTTGAAGCTCTGGAAAACCGTGTCCAGGCGCGGAAGTGGTACAAAGCTGCAATCAGAGCCGATCCTTTATGTTATGAGGCATTGGAATGTCTTATTGACAACCATATGCTGACATGTGAGGAAGAGGCAAGTCTACTTTCGTCGATACAATTTGGTCCTCAAGATGGATGGCTTTCGTCATTTTACTCGTGCTTGATAAAAAAGTATGACAAAGAAAATGTTGTAGAAGCAAACTTCGGAGAACTTGAAAAAGAGAGATCTACTAGTACCACTTCTGATCTTTCCTTCATTCACACTCTGAAAACCAACACTGACCTTCTGGCTTGCAAAGCAGAATACTACCATCAGTGCGGTGAATATCAAAAATGCTTTGAACTCACCTCTTTCCTACTTGAAAAAGACACTTTTCATCTCAAAAGTACATTAGTGCATCTAGCTGCCGCTATGGAACTTGGGAATTCAAATGAGCTCTATCTTTTGGCATGCAATTTAGTGAAGGACTATCCTCAAAAGGCTTTATCATGGTTTTCTGTTGGATGTTACTATTATTGTATCAAAAAGTATGATCAGTCACGCCGCTATTTCAGTAAGGCTACCAATGTAGATGGAAACTTTCCACCTGCATGGATAGGATATGGGAATGCTTATTCTGCTCAAGATGAGGGAGATCAAGCAATGTCAATTTATCGTACTGCTGCTCGTTTGTTTCCAGGGTGTCATTTGCCAACTTTGTACATTGGAATGGAGTACATGCGAACCCACAGTTACAAGCTCGCTGATCAGTTTTTGGTGCAGGCTAAGACTATTTGCCCATCGGATCCTCTTGTATATAATGAACTTGGAGTTGTTGCTTATCAAATGAAGGATTATAAGAAAGCTCTGTGGTGGTTTCAGAAAACTCTGGCGCAAATTCCTTCCCCTTTAGGTGAAATGTGGGAACCAACTATAGTTAATCTCGCCCATGTATACCGAAAATTGAAGATGTACAATGAAGCTATTTCATACTACGAGAAGGCACTTGCATTATCAACAAGAAGTGTGAGTACTTATGCAGGTCTCGCATATGCTTACCATTTGCAGGACAATTTTACTGCAGCAATAACATACTATCATAAAGCTTTGTGGATAAAGCCAGACGATCAGTTCTGTACTGAAATGCTAAGTTTAGCTTTAGCAGATGAATGCCATAACAATATAGACACAGAACGGATTAAGGCTTATGTTTGA

Protein Analysis

548

Amino Acids

63.08

Weight (kDa)

6.08

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 19 - 97 4e-21 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1287
AarI CACCTGC 1 cut(s) 1045
Acc36I ACCTGC 2 cut(s) 1045, 1460
AccI GTMKAC 4 cut(s) 204, 318, 550, 1383
AccII CGCG 3 cut(s) 171, 210, 448
AclWI GGATC 3 cut(s) 473, 1229, 1242
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 2 cut(s) 874, 1326
AcuI CTGAAG 1 cut(s) 275
AfiI CCNNNNNNNGG 4 cut(s) 34, 442, 581, 1495
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 6 cut(s) 422, 665, 791, 812, 879, 1396
AhlI ACTAGT 1 cut(s) 680
AjnI CCWGG 2 cut(s) 441, 1126
Alw21I GWGCWC 1 cut(s) 888
Alw26I GTCTC 2 cut(s) 36, 1478
AlwI GGATC 3 cut(s) 473, 1229, 1242
AoxI GGCC 2 cut(s) 141, 154
ApeKI GCWGC 4 cut(s) 464, 854, 1112, 1511
ApoI RAATTY 2 cut(s) 874, 1326
Asp700I GAANNNNTTC 1 cut(s) 331
AspLEI GCGC 3 cut(s) 173, 448, 1324
AspS9I GGNCC 3 cut(s) 37, 142, 572
AsuHPI GGTGA 6 cut(s) 43, 299, 374, 782, 787, 1355
AvaII GGWCC 2 cut(s) 37, 572
BamHI GGATCC 1 cut(s) 1234
BanII GRGCYC 2 cut(s) 117, 888
BarI GAAGNNNNNNTAC 2 cut(s) 1444, 1476
BauI CACGAG 1 cut(s) 604
Bbv12I GWGCWC 1 cut(s) 888
BbvI GCAGC 4 cut(s) 451, 841, 1099, 1523
BccI CCATC 5 cut(s) 575, 579, 768, 1016, 1237
BciT130I CCWGG 2 cut(s) 443, 1128
BclI TGATCA 2 cut(s) 982, 1195
BcoDI GTCTC 2 cut(s) 36, 1478
BcuI ACTAGT 1 cut(s) 680
BfaI CTAG 2 cut(s) 681, 851
BfmI CTRYAG 2 cut(s) 1362, 1509
BfuAI ACCTGC 2 cut(s) 1045, 1460
BglII AGATCT 1 cut(s) 674
BisI GCNGC 9 cut(s) 99, 157, 234, 465, 855, 858, 994, 1113, 1512
BlsI GCNGC 9 cut(s) 100, 158, 235, 466, 856, 859, 995, 1114, 1513
BmcAI AGTACT 1 cut(s) 1462
Bme1390I CCNGG 2 cut(s) 443, 1128
Bme18I GGWCC 2 cut(s) 37, 572
BmgT120I GGNCC 3 cut(s) 37, 142, 572
BmiI GGNNCC 2 cut(s) 1236, 1356
BmrFI CCNGG 2 cut(s) 443, 1128
BmsI GCATC 3 cut(s) 274, 280, 856
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 2 cut(s) 561, 1056
BsaAI YACGTR 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 1478
BsaJI CCNNGG 2 cut(s) 151, 1127
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 442, 581, 1495
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 1098
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 443, 1128
BseDI CCNNGG 2 cut(s) 151, 1127
BseGI GGATG 3 cut(s) 307, 590, 959
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 4 cut(s) 34, 442, 581, 1495
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 2 cut(s) 173, 181
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 4 cut(s) 451, 841, 1099, 1523
BsgI GTGCAG 1 cut(s) 1229
Bsh1236I CGCG 3 cut(s) 171, 210, 448
Bsh1285I CGRYCG 2 cut(s) 157, 208
BshFI GGCC 2 cut(s) 143, 156
BsiEI CGRYCG 2 cut(s) 157, 208
BsiHKAI GWGCWC 1 cut(s) 888
BslI CCNNNNNNNGG 4 cut(s) 34, 442, 581, 1495
BsmAI GTCTC 2 cut(s) 36, 1478
BsmI GAATGC 2 cut(s) 1063, 1604
BsnI GGCC 2 cut(s) 143, 156
Bso31I GGTCTC 1 cut(s) 1478
Bsp1286I GDGCHC 2 cut(s) 117, 888
Bsp1407I TGTACA 2 cut(s) 1149, 1401
BspANI GGCC 2 cut(s) 143, 156
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 3 cut(s) 171, 210, 448
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 1236, 1356
BspMAI CTGCAG 1 cut(s) 1513
BspMI ACCTGC 2 cut(s) 1045, 1460
BspPI GGATC 3 cut(s) 473, 1229, 1242
BspQI GCTCTTC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 1478
BsrDI GCAATG 1 cut(s) 1098
BsrGI TGTACA 2 cut(s) 1149, 1401
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 151, 1127
BssNAI GTATAC 2 cut(s) 319, 1384
BssSI CACGAG 1 cut(s) 604
Bst1107I GTATAC 2 cut(s) 319, 1384
Bst2BI CACGAG 1 cut(s) 604
Bst2UI CCWGG 2 cut(s) 443, 1128
Bst4CI ACNGT 3 cut(s) 167, 437, 1181
Bst6I CTCTTC 3 cut(s) 152, 243, 534
BstAUI TGTACA 2 cut(s) 1149, 1401
BstBAI YACGTR 1 cut(s) 407
BstC8I GCNNGC 6 cut(s) 117, 135, 742, 901, 1191, 1212
BstDEI CTNAG 3 cut(s) 138, 1215, 1577
BstF5I GGATG 3 cut(s) 307, 590, 959
BstFNI CGCG 3 cut(s) 171, 210, 448
BstHHI GCGC 3 cut(s) 173, 448, 1324
BstMAI GTCTC 2 cut(s) 36, 1478
BstMCI CGRYCG 2 cut(s) 157, 208
BstMWI GCNNNNNNNGC 5 cut(s) 422, 473, 746, 1067, 1475
BstNI CCWGG 2 cut(s) 443, 1128
BstNSI RCATGY 4 cut(s) 133, 534, 903, 1171
BstSCI CCNGG 2 cut(s) 441, 1126
BstSFI CTRYAG 2 cut(s) 1362, 1509
BstUI CGCG 3 cut(s) 171, 210, 448
BstV1I GCAGC 4 cut(s) 451, 841, 1099, 1523
BstX2I RGATCY 2 cut(s) 674, 1234
BstXI CCANNNNNNTGG 1 cut(s) 1041
BstYI RGATCY 2 cut(s) 674, 1234
BstZ17I GTATAC 2 cut(s) 319, 1384
BstZI CGGCCG 1 cut(s) 154
BsuRI GGCC 2 cut(s) 143, 156
BtsCI GGATG 3 cut(s) 307, 590, 959
BtsIMutI CAGTG 2 cut(s) 726, 770
BveI ACCTGC 2 cut(s) 1045, 1460
Cac8I GCNNGC 6 cut(s) 117, 135, 742, 901, 1191, 1212
CciI TCATGA 1 cut(s) 370
CfoI GCGC 3 cut(s) 173, 448, 1324
Cfr13I GGNCC 3 cut(s) 37, 142, 572
CseI GACGC 1 cut(s) 37
DdeI CTNAG 3 cut(s) 138, 1215, 1577
EaeI YGGCCR 1 cut(s) 154
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 3 cut(s) 152, 243, 534
EarI CTCTTC 3 cut(s) 152, 243, 534
Ecl136II GAGCTC 1 cut(s) 886
EclXI CGGCCG 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 117, 888
Eco31I GGTCTC 1 cut(s) 1478
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 37, 572
Eco52I CGGCCG 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 886
EcoO109I RGGNCCY 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 2 cut(s) 441, 1126
EcoRV GATATC 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 117, 888
FalI AAGNNNNNCTT 2 cut(s) 1570, 1602
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 2 cut(s) 522, 1480
FbaI TGATCA 2 cut(s) 982, 1195
FblI GTMKAC 4 cut(s) 204, 318, 550, 1383
Fnu4HI GCNGC 9 cut(s) 99, 157, 234, 465, 855, 858, 994, 1113, 1512
FokI GGATG 3 cut(s) 314, 597, 966
FriOI GRGCYC 2 cut(s) 117, 888
Fsp4HI GCNGC 9 cut(s) 99, 157, 234, 465, 855, 858, 994, 1113, 1512
FspBI CTAG 2 cut(s) 681, 851
GlaI GCGC 3 cut(s) 172, 447, 1323
GluI GCNGC 9 cut(s) 99, 157, 234, 465, 855, 858, 994, 1113, 1512
HaeIII GGCC 2 cut(s) 143, 156
HgaI GACGC 1 cut(s) 37
HhaI GCGC 3 cut(s) 173, 448, 1324
Hin6I GCGC 3 cut(s) 171, 446, 1322
HinP1I GCGC 3 cut(s) 171, 446, 1322
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 1533
HinfI GANTC 1 cut(s) 332
HphI GGTGA 6 cut(s) 43, 299, 374, 782, 787, 1355
Hpy166II GTNNAC 4 cut(s) 205, 319, 551, 1384
Hpy188I TCNGA 7 cut(s) 41, 473, 656, 694, 717, 1234, 1311
Hpy188III TCNNGA 4 cut(s) 371, 428, 578, 1073
Hpy8I GTNNAC 4 cut(s) 205, 319, 551, 1384
Hpy99I CGWCG 4 cut(s) 73, 171, 206, 562
HpyAV CCTTC 8 cut(s) 184, 250, 712, 743, 907, 1273, 1341, 1423
HpyCH4III ACNGT 3 cut(s) 167, 437, 1181
HpyCH4IV ACGT 2 cut(s) 315, 406
HpyF10VI GCNNNNNNNGC 5 cut(s) 422, 473, 746, 1067, 1475
HpyF3I CTNAG 3 cut(s) 138, 1215, 1577
HpySE526I ACGT 2 cut(s) 315, 406
HspAI GCGC 3 cut(s) 171, 446, 1322
Ksp22I TGATCA 2 cut(s) 982, 1195
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 4 cut(s) 451, 841, 1099, 1523
LweI GCATC 3 cut(s) 274, 280, 856
MaeI CTAG 2 cut(s) 681, 851
MaeII ACGT 2 cut(s) 315, 406
MaeIII GTNAC 4 cut(s) 49, 956, 987, 1181
MboII GAAGA 7 cut(s) 70, 73, 139, 260, 377, 551, 1408
MflI RGATCY 2 cut(s) 674, 1234
MhlI GDGCHC 2 cut(s) 117, 888
MluCI AATT 7 cut(s) 566, 874, 904, 1098, 1326, 1391, 1501
MmeI TCCRAC 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 331
MseI TTAA 3 cut(s) 276, 1368, 1629
MslI CAYNNNNRTG 3 cut(s) 502, 1039, 1157
MspR9I CCNGG 2 cut(s) 443, 1128
Mva1269I GAATGC 2 cut(s) 1063, 1604
MvaI CCWGG 2 cut(s) 443, 1128
MvnI CGCG 3 cut(s) 171, 210, 448
MwoI GCNNNNNNNGC 5 cut(s) 422, 473, 746, 1067, 1475
NdeI CATATG 2 cut(s) 522, 1480
NlaIV GGNNCC 2 cut(s) 1236, 1356
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 49, 987
NspI RCATGY 4 cut(s) 133, 534, 903, 1171
PaeI GCATGC 1 cut(s) 903
PagI TCATGA 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 1045
PciI ACATGT 1 cut(s) 530
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 2 cut(s) 1063, 1604
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 9 cut(s) 100, 158, 235, 466, 856, 859, 995, 1114, 1513
Ppu21I YACGTR 1 cut(s) 407
PscI ACATGT 1 cut(s) 530
PsiI TTATAA 1 cut(s) 1287
Psp124BI GAGCTC 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 441, 1126
PspGI CCWGG 2 cut(s) 441, 1126
PspN4I GGNNCC 2 cut(s) 1236, 1356
PspPI GGNCC 3 cut(s) 37, 142, 572
PstI CTGCAG 1 cut(s) 1513
PsuI RGATCY 2 cut(s) 674, 1234
PsyI GACNNNGTC 1 cut(s) 47
RseI CAYNNNNRTG 3 cut(s) 502, 1039, 1157
SacI GAGCTC 1 cut(s) 888
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 276, 1368, 1629
SatI GCNGC 9 cut(s) 99, 157, 234, 465, 855, 858, 994, 1113, 1512
Sau96I GGNCC 3 cut(s) 37, 142, 572
ScaI AGTACT 1 cut(s) 1462
ScrFI CCNGG 2 cut(s) 443, 1128
SduI GDGCHC 2 cut(s) 117, 888
SfaNI GCATC 3 cut(s) 274, 280, 856
SfcI CTRYAG 2 cut(s) 1362, 1509
SinI GGWCC 2 cut(s) 37, 572
SmiMI CAYNNNNRTG 3 cut(s) 502, 1039, 1157
SmlI CTYRAG 2 cut(s) 576, 1071
SmoI CTYRAG 2 cut(s) 576, 1071
SpeI ACTAGT 1 cut(s) 680
SphI GCATGC 1 cut(s) 903
Sse9I AATT 7 cut(s) 566, 874, 904, 1098, 1326, 1391, 1501
SspMI CTAG 2 cut(s) 681, 851
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 2 cut(s) 441, 1126
TaaI ACNGT 3 cut(s) 167, 437, 1181
TaiI ACGT 2 cut(s) 318, 409
TaqI TCGA 3 cut(s) 204, 246, 560
TasI AATT 7 cut(s) 566, 874, 904, 1098, 1326, 1391, 1501
TatI WGTACW 7 cut(s) 345, 836, 1149, 1164, 1401, 1460, 1565
TauI GCSGC 5 cut(s) 101, 159, 236, 860, 996
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 276, 1368, 1629
Tru9I TTAA 3 cut(s) 276, 1368, 1629
TscAI CASTG 2 cut(s) 733, 770
TseFI GTSAC 2 cut(s) 49, 987
TseI GCWGC 4 cut(s) 464, 854, 1112, 1511
Tsp45I GTSAC 2 cut(s) 49, 987
TspDTI ATGAA 9 cut(s) 318, 387, 694, 815, 1266, 1292, 1407, 1422, 1611
TspGWI ACGGA 3 cut(s) 17, 206, 1639
TspRI CASTG 2 cut(s) 733, 770
Tth111I GACNNNGTC 1 cut(s) 47
VpaK11BI GGWCC 2 cut(s) 37, 572
XapI RAATTY 2 cut(s) 874, 1326
XceI RCATGY 4 cut(s) 133, 534, 903, 1171
XmiI GTMKAC 4 cut(s) 204, 318, 550, 1383
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 2 cut(s) 681, 851
ZrmI AGTACT 1 cut(s) 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.