FvH4_3g22870

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014255
Physical Location & Seq
Reverse (-)
16022793 .. 16027039
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22870.t1

Sequence Viewer

Length: 537 bp
ATGTCGAAGTGGCTGGGGAGGATTGCTGGGATGTTTAGGAGTGGGAGTATGGTCGGAAAGGACAAAGCCGGCAATCGTTACTTCACCAGAAACGATGAAGTTGATGGCATCTTGAAAGAGAAAAGATGGGTGGTATTCAAGGGGGAGGAAGATCCAACCTCTGTTCCAGTTGAATGGATATGTTGGCTCAATGGGCAGCGCAAAAAGGCTCCAACGGCAGAGGAAATTATAGAATTGGAAGCAAAGCGTGAACGTGTCCGGCAAAATGTTGCTCTTTTGAAGAAAGAAGAAGAGGAAAGAAAAGCAAGAGAAGGCACTACACGGAAAGTCGTTAATACTGGTAAAGGAGGTCCAGACTTGAAAAGTTTCATTCGCCAATTTCCTGGGTCCTCAGAAGGTGACAAACACAAGGACGAAACCAATGAGATGGATGGAGTGAGGACTTCCAAAGAGAAAGAGGCAGTAAAACAAGAGTCTACAGAGCCAACAGGATCTGGTCAAACATTCAAGCCAGGGACATGGCAGCCACCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

179

Amino Acids

20.17

Weight (kDa)

9.25

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 15 - 91 2.7e-13 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 476
AclWI GGATC 2 cut(s) 146, 499
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 6 cut(s) 115, 139, 173, 280, 361, 508
AjnI CCWGG 2 cut(s) 382, 511
AlwI GGATC 2 cut(s) 146, 499
AlwNI CAGNNNCTG 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 196, 523
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 350, 387
AsuHPI GGTGA 2 cut(s) 76, 410
AvaII GGWCC 2 cut(s) 350, 387
BbvI GCAGC 1 cut(s) 208
BccI CCATC 4 cut(s) 98, 120, 421, 425
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 2 cut(s) 384, 513
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 2 cut(s) 197, 524
BlsI GCNGC 2 cut(s) 198, 525
Bme1390I CCNGG 2 cut(s) 384, 513
Bme18I GGWCC 2 cut(s) 350, 387
BmgT120I GGNCC 2 cut(s) 350, 387
BmiI GGNNCC 2 cut(s) 210, 388
BmrFI CCNGG 2 cut(s) 384, 513
BmsI GCATC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 383, 512
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 2 cut(s) 167, 343
BseBI CCWGG 2 cut(s) 384, 513
BseDI CCNNGG 2 cut(s) 383, 512
BseGI GGATG 2 cut(s) 36, 436
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 2 cut(s) 167, 343
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 2 cut(s) 13, 26
BsiSI CCGG 2 cut(s) 69, 259
BslFI GGGAC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 529
Bsp143I GATC 2 cut(s) 151, 491
BspCNI CTCAG 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 210, 388
BspPI GGATC 2 cut(s) 146, 499
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 2 cut(s) 167, 343
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 383, 512
BssMI GATC 2 cut(s) 151, 491
Bst2UI CCWGG 2 cut(s) 384, 513
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 391
BstF5I GGATG 2 cut(s) 36, 436
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 2 cut(s) 154, 494
BstMBI GATC 2 cut(s) 151, 491
BstMWI GCNNNNNNNGC 2 cut(s) 193, 215
BstNI CCWGG 2 cut(s) 384, 513
BstSCI CCNGG 2 cut(s) 382, 511
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 208
BstX2I RGATCY 2 cut(s) 151, 491
BstXI CCANNNNNNTGG 4 cut(s) 174, 383, 427, 519
BstYI RGATCY 2 cut(s) 151, 491
BtsCI GGATG 2 cut(s) 36, 436
Cac8I GCNNGC 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 350, 387
CviAII CATG 2 cut(s) 519, 534
CviJI RGCY 7 cut(s) 13, 68, 187, 209, 484, 511, 526
CviKI_1 RGCY 7 cut(s) 13, 68, 187, 209, 484, 511, 526
DdeI CTNAG 1 cut(s) 391
DpnI GATC 2 cut(s) 153, 493
DpnII GATC 2 cut(s) 151, 491
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 350, 387
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 382, 511
FaeI CATG 2 cut(s) 522, 537
FaiI YATR 5 cut(s) 50, 181, 230, 520, 535
FaqI GGGAC 1 cut(s) 529
FatI CATG 2 cut(s) 518, 533
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 2 cut(s) 197, 524
FokI GGATG 2 cut(s) 43, 443
Fsp4HI GCNGC 2 cut(s) 197, 524
GlaI GCGC 1 cut(s) 200
GluI GCNGC 2 cut(s) 197, 524
GsaI CCCAGC 2 cut(s) 17, 30
HapII CCGG 2 cut(s) 69, 259
HhaI GCGC 1 cut(s) 201
Hin1II CATG 2 cut(s) 522, 537
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HinfI GANTC 1 cut(s) 473
HpaII CCGG 2 cut(s) 69, 259
HphI GGTGA 2 cut(s) 76, 410
Hpy166II GTNNAC 2 cut(s) 251, 477
Hpy188I TCNGA 2 cut(s) 56, 394
Hpy188III TCNNGA 2 cut(s) 112, 353
Hpy8I GTNNAC 2 cut(s) 251, 477
HpyAV CCTTC 2 cut(s) 305, 389
HpyCH4IV ACGT 1 cut(s) 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 215
HpyF3I CTNAG 1 cut(s) 391
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 2 cut(s) 522, 537
HspAI GCGC 1 cut(s) 199
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 151, 491
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 1 cut(s) 117
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 2 cut(s) 77, 398
MalI GATC 2 cut(s) 153, 493
MboI GATC 2 cut(s) 151, 491
MboII GAAGA 4 cut(s) 161, 292, 299, 302
MflI RGATCY 2 cut(s) 151, 491
MluCI AATT 3 cut(s) 225, 233, 377
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 3 cut(s) 34, 179, 236
MnlI CCTC 9 cut(s) 12, 139, 169, 214, 286, 341, 400, 432, 451
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 532
MspI CCGG 2 cut(s) 69, 259
MspR9I CCNGG 2 cut(s) 384, 513
MvaI CCWGG 2 cut(s) 384, 513
MwoI GCNNNNNNNGC 2 cut(s) 193, 215
NaeI GCCGGC 1 cut(s) 70
NdeII GATC 2 cut(s) 151, 491
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 522, 537
NlaIV GGNNCC 2 cut(s) 210, 388
NmuCI GTSAC 1 cut(s) 398
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 365
PkrI GCNGC 2 cut(s) 198, 525
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 2 cut(s) 382, 511
PspFI CCCAGC 2 cut(s) 13, 26
PspGI CCWGG 2 cut(s) 382, 511
PspN4I GGNNCC 2 cut(s) 210, 388
PspPI GGNCC 2 cut(s) 350, 387
PspPPI RGGWCCY 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 2 cut(s) 151, 491
RseI CAYNNNNRTG 1 cut(s) 532
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 2 cut(s) 197, 524
Sau3AI GATC 2 cut(s) 151, 491
Sau96I GGNCC 2 cut(s) 350, 387
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 384, 513
SetI ASST 4 cut(s) 161, 256, 352, 400
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 350, 387
SmiMI CAYNNNNRTG 1 cut(s) 532
Sse9I AATT 3 cut(s) 225, 233, 377
StyD4I CCNGG 2 cut(s) 382, 511
TaiI ACGT 1 cut(s) 256
TaqI TCGA 1 cut(s) 5
TasI AATT 3 cut(s) 225, 233, 377
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 2 cut(s) 196, 523
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 2 cut(s) 111, 358
TspGWI ACGGA 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 350, 387
XmiI GTMKAC 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.