MD11G1216700.v1.1

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014255
Physical Location & Seq
Forward (+)
31608297 .. 31612518
4222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1216700.v1.1.491

Sequence Viewer

Length: 552 bp
ATGTCGAAGCTGTTTCAGAAGGTTGCTGGGTTCTTCACCAATCGGACTATGGTGGGAATGGACAAAGCTGGCAACCGTTACTTCGCCAGAAAGGAGGAGGTCGATGGCATCATGAAAGAGAAAAGATGGGTGGCATTCAAGGGCGAGGACGATCCGACCTCAATTCCAGTTGAGTGGATATGTTGGCTCAACGGGCAGAGGAAAAGGGCTCCAACACCGGAGGAACTGGCAGAATTGGAAGCTAGACGTGAACGTGTCCGGCAGAATGTTGCTCTTCTAAAGAAAGAAGAAGAGGAAAGGAAAGCAAGAGAAGGCAGTACGCGCAAAGTCATCAACACCGATAAAGTTGGAGGTCCAGACTTGAAAAGTTTCATTCAGCAATTTCCTGGGTCTTCTGAAGGTGGCGTAAATCCGGAAGAATCTGTTGCCACGGAAGGAGTGAGGAACTCCAAAGAGAAAGAGGCAAAGAAAGCAAAAGAAGCTCCGGAGTCGACAGAGCCAACAGGATCTGGTCAAACATTCAGGCCAGGGACATGGCAGCCGCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

184

Amino Acids

20.63

Weight (kDa)

9.14

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 16 - 91 5.1e-12 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 491
AccII CGCG 1 cut(s) 322
AccIII TCCGGA 2 cut(s) 412, 484
AciI CCGC 1 cut(s) 542
AclWI GGATC 2 cut(s) 146, 514
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 2 cut(s) 139, 364
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 2 cut(s) 385, 526
AluBI AGCT 4 cut(s) 10, 68, 242, 482
AluI AGCT 4 cut(s) 10, 68, 242, 482
AlwI GGATC 2 cut(s) 146, 514
AlwNI CAGNNNCTG 1 cut(s) 509
Aor13HI TCCGGA 2 cut(s) 412, 484
AoxI GGCC 1 cut(s) 524
ApeKI GCWGC 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 28
AvaII GGWCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 384
BccI CCATC 2 cut(s) 98, 120
BciT130I CCWGG 2 cut(s) 387, 528
BfaI CTAG 1 cut(s) 243
BisI GCNGC 2 cut(s) 539, 542
BlsI GCNGC 2 cut(s) 540, 543
Bme1390I CCNGG 2 cut(s) 387, 528
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 2 cut(s) 387, 528
BmsI GCATC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 384
BsaJI CCNNGG 3 cut(s) 386, 429, 527
BsaWI WCCGGW 3 cut(s) 217, 412, 484
Bse1I ACTGG 2 cut(s) 167, 231
BseAI TCCGGA 2 cut(s) 412, 484
BseBI CCWGG 2 cut(s) 387, 528
BseDI CCNNGG 3 cut(s) 386, 429, 527
BseNI ACTGG 2 cut(s) 167, 231
BseRI GAGGAG 1 cut(s) 110
BseYI CCCAGC 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 526
BsiSI CCGG 4 cut(s) 218, 259, 413, 485
BslFI GGGAC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 544
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 526
Bsp1286I GDGCHC 1 cut(s) 211
Bsp13I TCCGGA 2 cut(s) 412, 484
Bsp143I GATC 2 cut(s) 151, 506
BspACI CCGC 1 cut(s) 542
BspANI GGCC 1 cut(s) 526
BspEI TCCGGA 2 cut(s) 412, 484
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 210
BspPI GGATC 2 cut(s) 146, 514
BspQI GCTCTTC 1 cut(s) 279
BsrI ACTGG 2 cut(s) 167, 231
BssECI CCNNGG 3 cut(s) 386, 429, 527
BssMI GATC 2 cut(s) 151, 506
Bst2UI CCWGG 2 cut(s) 387, 528
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 279, 285
BstC8I GCNNGC 1 cut(s) 70
BstDSI CCRYGG 1 cut(s) 429
BstFNI CGCG 1 cut(s) 322
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 2 cut(s) 154, 509
BstMBI GATC 2 cut(s) 151, 506
BstMWI GCNNNNNNNGC 4 cut(s) 193, 321, 470, 479
BstNI CCWGG 2 cut(s) 387, 528
BstSCI CCNGG 2 cut(s) 385, 526
BstUI CGCG 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 506
BstXI CCANNNNNNTGG 2 cut(s) 174, 534
BstYI RGATCY 1 cut(s) 506
BsuRI GGCC 1 cut(s) 526
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 111
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 353
Csp6I GTAC 1 cut(s) 318
CviAII CATG 3 cut(s) 112, 534, 549
CviJI RGCY 9 cut(s) 10, 68, 187, 209, 242, 482, 499, 526, 541
CviKI_1 RGCY 9 cut(s) 10, 68, 187, 209, 242, 482, 499, 526, 541
CviQI GTAC 1 cut(s) 318
DpnI GATC 2 cut(s) 153, 508
DpnII GATC 2 cut(s) 151, 506
Eam1104I CTCTTC 2 cut(s) 279, 285
EarI CTCTTC 2 cut(s) 279, 285
Eco24I GRGCYC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 417
EcoRII CCWGG 2 cut(s) 385, 526
EcoT38I GRGCYC 1 cut(s) 211
FaeI CATG 3 cut(s) 115, 537, 552
FaiI YATR 5 cut(s) 50, 113, 181, 535, 550
FaqI GGGAC 1 cut(s) 544
FatI CATG 3 cut(s) 111, 533, 548
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 2 cut(s) 539, 542
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 2 cut(s) 539, 542
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 323
GluI GCNGC 2 cut(s) 539, 542
GsaI CCCAGC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 526
HapII CCGG 4 cut(s) 218, 259, 413, 485
HhaI GCGC 1 cut(s) 324
Hin1II CATG 3 cut(s) 115, 537, 552
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 2 cut(s) 419, 488
HpaII CCGG 4 cut(s) 218, 259, 413, 485
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 251, 492
Hpy188I TCNGA 4 cut(s) 18, 45, 156, 397
Hpy188III TCNNGA 4 cut(s) 112, 356, 413, 485
Hpy8I GTNNAC 2 cut(s) 251, 492
HpyAV CCTTC 4 cut(s) 13, 305, 392, 428
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 247, 253
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 321, 470, 479
HpySE526I ACGT 2 cut(s) 247, 253
Hsp92II CATG 3 cut(s) 115, 537, 552
HspAI GCGC 1 cut(s) 322
Kpn2I TCCGGA 2 cut(s) 412, 484
Kzo9I GATC 2 cut(s) 151, 506
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 2 cut(s) 214, 487
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 247, 253
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 153, 508
MboI GATC 2 cut(s) 151, 506
MboII GAAGA 6 cut(s) 25, 266, 299, 302, 384, 428
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 3 cut(s) 162, 233, 380
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 179, 236, 328
MroI TCCGGA 2 cut(s) 412, 484
MroXI GAANNNNTTC 1 cut(s) 368
MspI CCGG 4 cut(s) 218, 259, 413, 485
MspR9I CCNGG 2 cut(s) 387, 528
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 2 cut(s) 387, 528
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 4 cut(s) 193, 321, 470, 479
NdeII GATC 2 cut(s) 151, 506
NlaIII CATG 3 cut(s) 115, 537, 552
NlaIV GGNNCC 1 cut(s) 210
PagI TCATGA 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 279
PctI GAATGC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 2 cut(s) 540, 543
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp6I CCWGG 2 cut(s) 385, 526
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 385, 526
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 353
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 506
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SalI GTCGAC 1 cut(s) 490
SapI GCTCTTC 1 cut(s) 279
SatI GCNGC 2 cut(s) 539, 542
Sau3AI GATC 2 cut(s) 151, 506
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 2 cut(s) 387, 528
SduI GDGCHC 1 cut(s) 211
SfaNI GCATC 1 cut(s) 117
SinI GGWCC 1 cut(s) 353
Sse9I AATT 3 cut(s) 162, 233, 380
SsiI CCGC 1 cut(s) 542
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 385, 526
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 250, 256
TaqI TCGA 3 cut(s) 5, 102, 491
TasI AATT 3 cut(s) 162, 233, 380
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 1 cut(s) 419
TseI GCWGC 1 cut(s) 538
TspDTI ATGAA 2 cut(s) 128, 361
TspGWI ACGGA 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.