Rroxscaffold_1G00041110

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014255
Physical Location & Seq
Forward (+)
58862916 .. 58867813
4898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041110.1

Sequence Viewer

Length: 507 bp
ATGTCGAAGTGGTTGGGGAGGATTGCTGGGCTGTTCAGCAGTCGGAGTATGGTCGGAAAGGACAAGGCCGGGAACCGTTACTTCACCAGAAAAGATGAAGTTGATGGCGTCTTGAAAGAGAAAAGATGGGTGGTATTCAAGGGGGAGGAGGACCCAACCTCTGTTCCAGTTGAATGGATATGTTGGCTCAATGGGCAGCGGAAAAAGGCTCCAACAACAGAGGAAATGATAGAATTGGAAGCAAAGCGTGAAAGAGTCCGGCAAAATGTTGCTCTTCTGAAGAAAGAAGAAGAGGAAAGAAAAGCAAGAGAAGGCACTACACGCAAAGTCGTAAATACTGGTAAAGCTGGAGGCCCAGACTTGAGAAGTTTCATTCGCCAATTTCCTGGGTCCTCAGAAGGTGACGAACACAAGGAAGAAACCAATAAGATGGATGAAGTGAGGTCTACAGAGCCAACAGGATCTGGTCAAACATTCAAGCCAGGGACATGGCAGCCACCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

168

Amino Acids

19.16

Weight (kDa)

9.32

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 17 - 91 5.8e-13 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 446
AciI CCGC 1 cut(s) 199
AclWI GGATC 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 108
AgsI TTSAA 4 cut(s) 115, 139, 173, 478
AjnI CCWGG 2 cut(s) 385, 481
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
AlwI GGATC 1 cut(s) 469
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 2 cut(s) 66, 352
ApeKI GCWGC 2 cut(s) 196, 493
AspS9I GGNCC 3 cut(s) 151, 353, 390
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 76, 413
AvaII GGWCC 2 cut(s) 151, 390
BbvI GCAGC 1 cut(s) 208
BccI CCATC 3 cut(s) 98, 120, 424
BciT130I CCWGG 2 cut(s) 387, 483
BcnI CCSGG 1 cut(s) 70
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 2 cut(s) 197, 494
BlsI GCNGC 2 cut(s) 198, 495
Bme1390I CCNGG 3 cut(s) 70, 387, 483
Bme18I GGWCC 2 cut(s) 151, 390
BmgT120I GGNCC 3 cut(s) 151, 353, 390
BmiI GGNNCC 4 cut(s) 74, 153, 210, 391
BmrFI CCNGG 3 cut(s) 70, 387, 483
BpmI CTGGAG 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 386, 482
Bse1I ACTGG 2 cut(s) 167, 343
BseBI CCWGG 2 cut(s) 387, 483
BseDI CCNNGG 2 cut(s) 386, 482
BseGI GGATG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 167, 343
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 26
BshFI GGCC 2 cut(s) 68, 354
BsiSI CCGG 2 cut(s) 69, 259
BslFI GGGAC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 499
BsnI GGCC 2 cut(s) 68, 354
Bsp143I GATC 1 cut(s) 461
BspACI CCGC 1 cut(s) 199
BspANI GGCC 2 cut(s) 68, 354
BspCNI CTCAG 1 cut(s) 407
BspLI GGNNCC 4 cut(s) 74, 153, 210, 391
BspPI GGATC 1 cut(s) 469
BspQI GCTCTTC 1 cut(s) 279
BsrI ACTGG 2 cut(s) 167, 343
BssECI CCNNGG 2 cut(s) 386, 482
BssMI GATC 1 cut(s) 461
BssNI GRCGYC 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 387, 483
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 279, 285
BstACI GRCGYC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 1 cut(s) 464
BstMBI GATC 1 cut(s) 461
BstMWI GCNNNNNNNGC 2 cut(s) 193, 321
BstNI CCWGG 2 cut(s) 387, 483
BstSCI CCNGG 3 cut(s) 68, 385, 481
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 461
BstXI CCANNNNNNTGG 4 cut(s) 174, 386, 430, 489
BstYI RGATCY 1 cut(s) 461
BsuRI GGCC 2 cut(s) 68, 354
BtsCI GGATG 1 cut(s) 439
CaiI CAGNNNCTG 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 151, 353, 390
CseI GACGC 1 cut(s) 97
CviAII CATG 2 cut(s) 489, 504
CviJI RGCY 9 cut(s) 31, 68, 187, 209, 347, 354, 454, 481, 496
CviKI_1 RGCY 9 cut(s) 31, 68, 187, 209, 347, 354, 454, 481, 496
DdeI CTNAG 1 cut(s) 394
DpnI GATC 1 cut(s) 463
DpnII GATC 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 279, 285
EarI CTCTTC 2 cut(s) 279, 285
Eco47I GGWCC 2 cut(s) 151, 390
Eco57I CTGAAG 1 cut(s) 299
EcoO109I RGGNCCY 2 cut(s) 151, 390
EcoRII CCWGG 2 cut(s) 385, 481
FaeI CATG 2 cut(s) 492, 507
FaiI YATR 4 cut(s) 50, 181, 490, 505
FaqI GGGAC 1 cut(s) 499
FatI CATG 2 cut(s) 488, 503
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 2 cut(s) 197, 494
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 2 cut(s) 197, 494
GluI GCNGC 2 cut(s) 197, 494
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 2 cut(s) 68, 354
HapII CCGG 2 cut(s) 69, 259
HgaI GACGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 492, 507
HinfI GANTC 1 cut(s) 255
HpaII CCGG 2 cut(s) 69, 259
HphI GGTGA 2 cut(s) 76, 413
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 4 cut(s) 45, 56, 279, 397
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 447
HpyAV CCTTC 2 cut(s) 305, 392
HpyCH4III ACNGT 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 321
HpyF3I CTNAG 1 cut(s) 394
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 492, 507
Kzo9I GATC 1 cut(s) 461
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 208
MaeIII GTNAC 2 cut(s) 77, 401
MalI GATC 1 cut(s) 463
MboI GATC 1 cut(s) 461
MboII GAAGA 5 cut(s) 266, 292, 299, 302, 428
MflI RGATCY 1 cut(s) 461
MluCI AATT 2 cut(s) 233, 380
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 3 cut(s) 23, 34, 236
MnlI CCTC 9 cut(s) 12, 139, 142, 169, 214, 286, 344, 403, 435
MslI CAYNNNNRTG 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 2 cut(s) 69, 259
MspR9I CCNGG 3 cut(s) 70, 387, 483
MvaI CCWGG 2 cut(s) 387, 483
MwoI GCNNNNNNNGC 2 cut(s) 193, 321
NciI CCSGG 1 cut(s) 70
NdeII GATC 1 cut(s) 461
NlaIII CATG 2 cut(s) 492, 507
NlaIV GGNNCC 4 cut(s) 74, 153, 210, 391
NmuCI GTSAC 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 279
PkrI GCNGC 2 cut(s) 198, 495
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 2 cut(s) 151, 390
Psp5II RGGWCCY 2 cut(s) 151, 390
Psp6I CCWGG 2 cut(s) 385, 481
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 385, 481
PspN4I GGNNCC 4 cut(s) 74, 153, 210, 391
PspPI GGNCC 3 cut(s) 151, 353, 390
PspPPI RGGWCCY 2 cut(s) 151, 390
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 502
SapI GCTCTTC 1 cut(s) 279
SatI GCNGC 2 cut(s) 197, 494
Sau3AI GATC 1 cut(s) 461
Sau96I GGNCC 3 cut(s) 151, 353, 390
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 3 cut(s) 70, 387, 483
SetI ASST 4 cut(s) 161, 349, 403, 446
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 2 cut(s) 151, 390
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 2 cut(s) 233, 380
SsiI CCGC 1 cut(s) 199
StyD4I CCNGG 3 cut(s) 68, 385, 481
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 5
TasI AATT 2 cut(s) 233, 380
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 196, 493
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 111, 361, 450
VpaK11BI GGWCC 2 cut(s) 151, 390
XmiI GTMKAC 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.