Rorug05G0183900

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014255
Physical Location & Seq
Reverse (-)
17771936 .. 17776462
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0183900.1

Sequence Viewer

Length: 3525 bp
ATGGCTGCTGCTTCTTCTAGCTCTGATATCACCCCTCAGGAGAAGCATGATGTTTTTCTTAGTTTCAGAGGCGAGGACACCCGGTATACTTTCACCAGCCATCTTTATGAGGCTTTATGTAAGAAGAAAATCCGAACCTACATAGATAACAAACTACAGAGAGGAGATGAAATCGCACCTGCCCTTCTCGAAGTAATCAGTGAATCGAAGCTTTCGGTTATCATTTTCTCCAAAAACTATGCTTCTTCTACATGGTGCTTGGATGAACTTGCGCACATACTCGAATGTAGAGATAAAGCGCCCATACTCGAATCCAAAGATAAACAGTTTATTATACCCATCTTCTACGGCGTAGATCCATCAGATGTACTCAAACAATCGGGGAGTTATGCAGATGCATTTGTTGAACATGAGAAACGTTTCGAGGACATGAGGGACAAGGTGCTCAGGTGGAGAAATGCTTTGACAACAGCAGCCAGTATATCTGGGCTTCATTCCCAAACAGATAGGCCCGAGTCTGAATTAGTTAAGACTGTGGTCAATGACATTTTGAGGAAATTGAACAAATTGGAAATCGCAAGGGCTTTAGATGGCCTGGTTGGAATGGAAAAACACATTCAACAAGTTGAATTTTTATTACGCTTGGAGTCCCGGGATGTCCAGATTGTAGGTATTTGGGGTATGGGTGGTATCGGTAAGACCACCATTGCTGATGTTGTATTTCACCGTGTCTCTTCTCAATTCGAAGCTTGCTGCTTTGTTGCAAATGTTAGGGAAGCATCAGAGAACCGTGGACTATATGATTTGCGAAATGAACTTTTTCGTACTTTACTGGAGGACGAAAACATTTGCATAACCACCCCCTCTATCGGATCAACTTCTATGACAAAGAGGCTTCGCTGTACAAAGGCTCTCATTGTTCTTGATGATGTGAGTGAGTTTTCCCAATTAGAATTTTTAGCTGGAGGTGATTGCAATCTGTTTGGCCTTGGAAGTAGAATCATTGTAACAATGAGAAATAAACGCGTACTTGGGCACGGTGTTGATGATGATAAGATATATGAGGTTAAGAAATTAGACGTTGATGAAGCTCTTGAGCTCTTTTATTCGAGAGCTTTTAGAAATATCTCTTCCCAAGATGATTGTACAACTTTTGCAAAAGAGGTGGTAGATTATGCTGGAGGCAATCCATTGGCTCTTAAAATCTTGGGCGGAGTATTCTATCATTGCAAGAGCAAAGGAGACTGGGAAGGTGAATTAGATAAATTGAAAAAATTTCCTGACAAAAATATCCAGAATACATTGAGGTTAAGTTATGATAGATTAGGAGAAAATGAGAAGGAGATATTTCTCGATATTGCCTGTTGTCACAAAGGAAAGCATATTAATGAGGCAAAAAGAATTTTAAATGCCTGTGGTTTTTCTGCCAATGCGGGAGTAAGTCTTCTCATTGATATGTCTCTCATATCCGTGATTGGAGGTTCCGTTGAGGTGCATGATTTGATACAAGAAATGGGTCGGGAAATTGTTCGTTATGAATGTGTTAAAGATCCTGGAAAACGCAGTAGATTGTGGCTTGCTAATGATGTTCGTCGTGTACTGAAAAATAATATGGGTACTGAAGCCATTGAGTGCATAGTCATGAACACGGATCTCATTACAGATTTATACGTGAATGCTGCATCCTTCGAAAAGATGCATAATCTAAGATTACTAGATTTATATTCTGACGACTACTACTCGTACACCAGGGGTGTCCCCAAAGTGCACCTTCCTCCAGGTTTCGAGTTTCTACCTGAGGCCCTTAAATATCTGAGGTGGCATTTCTATCCTTTGACATCTATGCCAACGAGGTTTTCTTCAGAAAATCTTGTCGAGCTTCATATGCCCAACAGCCGACTTGAGCAACTTTGGACTAAAGGCGTGCAGATCAATCTTCGGAGCTTAAATCGCATCGATCTTCGTCTGTCCAAGCACCTGGCTGAGGTTCCAGATCTCTCTCAGAGTCCAAAACTCGAGAGCATTAATCTTGAAGGTTGTACAAGTTTGGTTCAACTTCTTTCGTCTTTTCAGCATCTTGCCAAGCTCACTTGGCTCAGTCTGGATGACTGCTCCAATCTCAAAATTCTCCCAGAGATGCCAGGCAATCTGGAAGTCTTAAGTTTGGAACGTACTGCAATAGAAGAATTGCCTTCATCAATTTGGTGTCTCGAGAAGCTCCGTTATTTGGGTCTTGATTCATGTAAAGAGCTTAAGAATATTCCAAGCAGCATTTGTAGGCTGAATTCGCTCCGATGTCTTCGTCTAAGTGGCTGCCTCTCTCTTGAGGAGCTACCTTCTGGGTTGATAACACTGAAATTGTACAACTGCAAGAGTCTTGGGTCTTTGTCTGTCGAGCTACCTTCTGGGCTAACGGGGCTGGACTTGAGCAATTGCAAGAGTCTTGGGTCTTTGCCTGTGGAGCTCCCTTCTGGGCTGACGGGGCTGGACTTGAGCGATTGTGAGAGTCTTGGGTCTTTGCCTGTCGAGCTCCCTTCTGGGCTGAGGAAACTGGACTTGAGAAATTGCAAGAGCCTTCGGTTTTTACCGAAGCTTCCATGGCGTTTAATAGACTTGGATGCACATGGCTGCACGTCACTAGAGACAGTCTCAAGTTCGATGAGGACTGCACCCATACAAGGTGAGGATGAGGATGTCAACAATTCAGAAGACCTCTGGCCTAAGGGTGAAGAGCGACTTGAATTTATAAATTGTGTGAAATTGGATCAGAGTGCACGGAGCAACATAATGGCTGATGCACAGCTTAGAGTTATGCGATATGCATCACGTTGCCTCAGTGAATGGAGTACGATTAAAATAGTTTGTTCGGGAAATGAAATTCCAAAGTGGATGAGGTATCAAAGTGAGGGATGTTTGATAAATATCAAGCTTCCTCCTCATTCGTTTCGTCAAGCCTTCCTCGGTTACGCTCTATGCATTGTGGTTGCATTCAACAACTATACGCCGCCACCCATTAATATTGGACCTACCTACTTTAGTTGTGGAACCCATCACAAAACTAACAATGGTCACAAAGATTGGTGCGACCTCGATATACGCCTACCAGATGATATAATTCTCAATTCAGATCATGTGTTCCTGTGGTATTTCTGCCCTAATTTTGTTCGATTTGGAAGTACAATCAATCGCGAGGATTGGGTAAAAGCATCCGAGGCCTCTTTTAACTTCATGTTGTACCAAGATACCGGTCCGAAAGAAATTCCCTTTGACCTCTCCACTGTGAGGGTGGAAAGGTGTGGAGTCTCCTTGCTGTATGCCCAAGATTTTGAAAAATTACTTGCAGGCATTGATGAAGATGTAGTTATGGAACCAAAACAAGTAGTTGAAGAAGAAGATATCATCATCAAAACCAAGAGACGATGGGATGAGTCTGAACTCAGTGGAAGTGTAACTGCAAGCTTCAAAGAAGAAGATAACGACCAACTACCTCCCCAGAGTAGTAAAAGGAAAATCAACATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

1174

Amino Acids

132.75

Weight (kDa)

5.59

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 201 9.7e-53 TIR domain
TIR_2 PF13676 18 - 136 1.1e-12 TIR domain
NB-ARC PF00931 204 - 375 1.2e-19 NB-ARC domain
WHD_ROQ1 PF23282 444 - 511 4.8e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 658 - 864 4.1e-09 Leucine-rich repeat region
C-JID PF20160 955 - 1109 1.4e-15 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2747
AarI CACCTGC 1 cut(s) 187
Acc16I TGCGCA 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 86
AccII CGCG 2 cut(s) 1026, 3189
AciI CCGC 3 cut(s) 1212, 1433, 3005
AclI AACGTT 1 cut(s) 418
AclWI GGATC 5 cut(s) 350, 880, 1544, 1659, 2772
AcsI RAATTY 9 cut(s) 629, 953, 1274, 1401, 2124, 2284, 2741, 2877, 3258
AcuI CTGAAG 2 cut(s) 1641, 1845
AfiI CCNNNNNNNGG 5 cut(s) 869, 1984, 2227, 2678, 3282
AflII CTTAAG 2 cut(s) 2158, 2252
AflIII ACRYGT 1 cut(s) 1024
AgeI ACCGGT 1 cut(s) 3245
AjiI CACGTC 1 cut(s) 2634
AjnI CCWGG 6 cut(s) 594, 1552, 1748, 1777, 1977, 2140
AjuI GAANNNNNNNTTGG 2 cut(s) 1840, 1872
Alw21I GWGCWC 6 cut(s) 447, 1101, 1770, 2466, 2532, 2776
Alw26I GTCTC 8 cut(s) 736, 1236, 1464, 2213, 2636, 2653, 3307, 3409
Alw44I GTGCAC 2 cut(s) 1766, 2772
AlwI GGATC 5 cut(s) 350, 880, 1544, 1659, 2772
Ama87I CYCGRG 4 cut(s) 512, 651, 2015, 2210
AoxI GGCC 6 cut(s) 509, 592, 985, 1800, 2717, 3213
ApaLI GTGCAC 2 cut(s) 1766, 2772
ApeKI GCWGC 8 cut(s) 5, 8, 473, 753, 1679, 2268, 2313, 2628
ApoI RAATTY 9 cut(s) 629, 953, 1274, 1401, 2124, 2284, 2741, 2877, 3258
AseI ATTAAT 3 cut(s) 1386, 2025, 3015
AsiGI ACCGGT 1 cut(s) 3245
Asp700I GAANNNNTTC 3 cut(s) 419, 819, 1527
AspLEI GCGC 2 cut(s) 274, 301
AspS9I GGNCC 4 cut(s) 510, 1801, 3023, 3248
AsuC2I CCSGG 3 cut(s) 82, 652, 653
AsuHPI GGTGA 7 cut(s) 22, 85, 716, 980, 1265, 2693, 2738
AsuII TTCGAA 2 cut(s) 744, 1689
AvaI CYCGRG 4 cut(s) 512, 651, 2015, 2210
AvaII GGWCC 2 cut(s) 3023, 3248
AxyI CCTNAGG 3 cut(s) 36, 1797, 2721
BaeGI GKGCMC 3 cut(s) 1038, 1770, 2776
BanII GRGCYC 3 cut(s) 1101, 2466, 2532
BbsI GAAGAC 3 cut(s) 1436, 2291, 2715
Bbv12I GWGCWC 6 cut(s) 447, 1101, 1770, 2466, 2532, 2776
BbvCI CCTCAGC 2 cut(s) 1983, 2543
BbvI GCAGC 6 cut(s) 485, 740, 1666, 2280, 2300, 2615
BccI CCATC 6 cut(s) 108, 347, 367, 584, 3057, 3414
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 6 cut(s) 596, 1554, 1750, 1779, 1979, 2142
BcnI CCSGG 3 cut(s) 82, 652, 653
BcoDI GTCTC 8 cut(s) 736, 1236, 1464, 2213, 2636, 2653, 3307, 3409
BfaI CTAG 3 cut(s) 18, 1715, 2639
BfmI CTRYAG 1 cut(s) 155
BfoI RGCGCY 1 cut(s) 302
BfrI CTTAAG 2 cut(s) 2158, 2252
BfuAI ACCTGC 1 cut(s) 187
BglII AGATCT 1 cut(s) 1993
BisI GCNGC 9 cut(s) 6, 9, 474, 754, 1680, 2269, 2314, 2629, 3005
BlsI GCNGC 9 cut(s) 7, 10, 475, 755, 1681, 2270, 2315, 2630, 3006
Bme1390I CCNGG 9 cut(s) 82, 596, 652, 653, 1554, 1750, 1779, 1979, 2142
Bme18I GGWCC 2 cut(s) 3023, 3248
BmeT110I CYCGRG 4 cut(s) 512, 651, 2015, 2210
BmgBI CACGTC 1 cut(s) 2634
BmgT120I GGNCC 4 cut(s) 510, 1801, 3023, 3248
BmiI GGNNCC 4 cut(s) 1483, 1989, 3046, 3369
BmrFI CCNGG 9 cut(s) 82, 596, 652, 653, 1554, 1750, 1779, 1979, 2142
BmrI ACTGGG 1 cut(s) 1255
BmuI ACTGGG 1 cut(s) 1255
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 3 cut(s) 1436, 2291, 2715
BplI GAGNNNNNCTC 2 cut(s) 2633, 2665
BpmI CTGGAG 4 cut(s) 854, 984, 1200, 1761
Bpu10I CCTNAGC 3 cut(s) 446, 1983, 2543
Bpu14I TTCGAA 2 cut(s) 744, 1689
BpuEI CTTGAG 7 cut(s) 1115, 1922, 2345, 2446, 2512, 2578, 2635
BpuMI CCSGG 3 cut(s) 82, 652, 653
Bsa29I ATCGAT 1 cut(s) 1956
BsaAI YACGTR 1 cut(s) 1672
BsaBI GATNNNNATC 3 cut(s) 975, 2821, 2921
BsaJI CCNNGG 7 cut(s) 651, 790, 988, 1749, 2597, 2959, 3210
BsaWI WCCGGW 1 cut(s) 3245
BsaXI ACNNNNNCTCC 2 cut(s) 1470, 1500
Bsc4I CCNNNNNNNGG 5 cut(s) 869, 1984, 2227, 2678, 3282
Bse118I RCCGGY 1 cut(s) 3245
Bse1I ACTGG 4 cut(s) 477, 837, 1250, 2556
Bse21I CCTNAGG 3 cut(s) 36, 1797, 2721
Bse3DI GCAATG 2 cut(s) 705, 1225
Bse8I GATNNNNATC 3 cut(s) 975, 2821, 2921
BseBI CCWGG 6 cut(s) 596, 1554, 1750, 1779, 1979, 2142
BseCI ATCGAT 1 cut(s) 1956
BseDI CCNNGG 7 cut(s) 651, 790, 988, 1749, 2597, 2959, 3210
BseJI GATNNNNATC 3 cut(s) 975, 2821, 2921
BseLI CCNNNNNNNGG 5 cut(s) 869, 1984, 2227, 2678, 3282
BseMI GCAATG 2 cut(s) 705, 1225
BseNI ACTGG 4 cut(s) 477, 837, 1250, 2556
BseRI GAGGAG 3 cut(s) 177, 2342, 2925
BseSI GKGCMC 3 cut(s) 1038, 1770, 2776
BseXI GCAGC 6 cut(s) 485, 740, 1666, 2280, 2300, 2615
BsgI GTGCAG 3 cut(s) 1946, 2614, 2652
Bsh1236I CGCG 2 cut(s) 1026, 3189
BshFI GGCC 6 cut(s) 511, 594, 987, 1802, 2719, 3215
BshTI ACCGGT 1 cut(s) 3245
BshVI ATCGAT 1 cut(s) 1956
BsiHKAI GWGCWC 6 cut(s) 447, 1101, 1770, 2466, 2532, 2776
BsiHKCI CYCGRG 4 cut(s) 512, 651, 2015, 2210
BsiSI CCGG 3 cut(s) 82, 652, 3246
BslFI GGGAC 3 cut(s) 449, 634, 1742
BslI CCNNNNNNNGG 5 cut(s) 869, 1984, 2227, 2678, 3282
BsmAI GTCTC 8 cut(s) 736, 1236, 1464, 2213, 2636, 2653, 3307, 3409
BsmBI CGTCTC 1 cut(s) 3409
BsmFI GGGAC 3 cut(s) 449, 634, 1742
BsmI GAATGC 2 cut(s) 1681, 2987
BsnI GGCC 6 cut(s) 511, 594, 987, 1802, 2719, 3215
BsoBI CYCGRG 4 cut(s) 512, 651, 2015, 2210
Bsp119I TTCGAA 2 cut(s) 744, 1689
Bsp1286I GDGCHC 7 cut(s) 447, 1038, 1101, 1770, 2466, 2532, 2776
Bsp1407I TGTACA 4 cut(s) 902, 1145, 2039, 2361
Bsp143I GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
Bsp19I CCATGG 1 cut(s) 2597
Bsp68I TCGCGA 1 cut(s) 3189
BspACI CCGC 3 cut(s) 1212, 1433, 3005
BspANI GGCC 6 cut(s) 511, 594, 987, 1802, 2719, 3215
BspDI ATCGAT 1 cut(s) 1956
BspFNI CGCG 2 cut(s) 1026, 3189
BspHI TCATGA 1 cut(s) 1641
BspLI GGNNCC 4 cut(s) 1483, 1989, 3046, 3369
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 5 cut(s) 350, 880, 1544, 1659, 2772
BspQI GCTCTTC 1 cut(s) 2724
BspT104I TTCGAA 2 cut(s) 744, 1689
BspTI CTTAAG 2 cut(s) 2158, 2252
BsrDI GCAATG 2 cut(s) 705, 1225
BsrFI RCCGGY 1 cut(s) 3245
BsrGI TGTACA 4 cut(s) 902, 1145, 2039, 2361
BsrI ACTGG 4 cut(s) 477, 837, 1250, 2556
BssAI RCCGGY 1 cut(s) 3245
BssECI CCNNGG 7 cut(s) 651, 790, 988, 1749, 2597, 2959, 3210
BssMI GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
BssNAI GTATAC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 988, 2597
Bst1107I GTATAC 1 cut(s) 87
Bst2UI CCWGG 6 cut(s) 596, 1554, 1750, 1779, 1979, 2142
Bst4CI ACNGT 7 cut(s) 327, 535, 728, 791, 1040, 2647, 3280
Bst6I CTCTTC 3 cut(s) 739, 1135, 2724
BstAFI CTTAAG 2 cut(s) 2158, 2252
BstAUI TGTACA 4 cut(s) 902, 1145, 2039, 2361
BstBAI YACGTR 1 cut(s) 1672
BstBI TTCGAA 2 cut(s) 744, 1689
BstC8I GCNNGC 5 cut(s) 751, 1578, 1925, 3343, 3457
BstDSI CCRYGG 2 cut(s) 790, 2597
BstENI CCTNNNNNAGG 1 cut(s) 1982
BstFNI CGCG 2 cut(s) 1026, 3189
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 2 cut(s) 274, 301
BstKTI GATC 9 cut(s) 358, 875, 1552, 1654, 1932, 1960, 1996, 2767, 3130
BstMAI GTCTC 8 cut(s) 736, 1236, 1464, 2213, 2636, 2653, 3307, 3409
BstMBI GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
BstNI CCWGG 6 cut(s) 596, 1554, 1750, 1779, 1979, 2142
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 9 cut(s) 80, 594, 650, 651, 1552, 1748, 1777, 1977, 2140
BstSFI CTRYAG 1 cut(s) 155
BstSLI GKGCMC 3 cut(s) 1038, 1770, 2776
BstUI CGCG 2 cut(s) 1026, 3189
BstV1I GCAGC 6 cut(s) 485, 740, 1666, 2280, 2300, 2615
BstV2I GAAGAC 3 cut(s) 1436, 2291, 2715
BstX2I RGATCY 4 cut(s) 355, 1549, 1651, 1993
BstXI CCANNNNNNTGG 1 cut(s) 1978
BstYI RGATCY 4 cut(s) 355, 1549, 1651, 1993
BstZ17I GTATAC 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 1956
Bsu36I CCTNAGG 3 cut(s) 36, 1797, 2721
BsuRI GGCC 6 cut(s) 511, 594, 987, 1802, 2719, 3215
BsuTUI ATCGAT 1 cut(s) 1956
BtgI CCRYGG 2 cut(s) 790, 2597
BtrI CACGTC 1 cut(s) 2634
BtsIMutI CAGTG 5 cut(s) 205, 2351, 2842, 3276, 3445
BtuMI TCGCGA 1 cut(s) 3189
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 5 cut(s) 751, 1578, 1925, 3343, 3457
CciI TCATGA 1 cut(s) 1641
CfoI GCGC 2 cut(s) 274, 301
Cfr10I RCCGGY 1 cut(s) 3245
Cfr13I GGNCC 4 cut(s) 510, 1801, 3023, 3248
Cfr9I CCCGGG 1 cut(s) 651
ClaI ATCGAT 1 cut(s) 1956
CpoI CGGWCCG 1 cut(s) 3248
CspAI ACCGGT 1 cut(s) 3245
CspCI CAANNNNNGTGG 2 cut(s) 1146, 1181
CspI CGGWCCG 1 cut(s) 3248
DpnI GATC 9 cut(s) 357, 874, 1551, 1653, 1931, 1959, 1995, 2766, 3129
DpnII GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
DraI TTTAAA 1 cut(s) 1407
Eam1104I CTCTTC 3 cut(s) 739, 1135, 2724
EarI CTCTTC 3 cut(s) 739, 1135, 2724
EciI GGCGGA 1 cut(s) 1227
Ecl136II GAGCTC 3 cut(s) 1099, 2464, 2530
Eco130I CCWWGG 2 cut(s) 988, 2597
Eco147I AGGCCT 1 cut(s) 3215
Eco24I GRGCYC 3 cut(s) 1101, 2466, 2532
Eco32I GATATC 2 cut(s) 28, 3397
Eco47I GGWCC 2 cut(s) 3023, 3248
Eco53kI GAGCTC 3 cut(s) 1099, 2464, 2530
Eco57I CTGAAG 2 cut(s) 1641, 1845
Eco81I CCTNAGG 3 cut(s) 36, 1797, 2721
Eco88I CYCGRG 4 cut(s) 512, 651, 2015, 2210
EcoICRI GAGCTC 3 cut(s) 1099, 2464, 2530
EcoNI CCTNNNNNAGG 1 cut(s) 1982
EcoO109I RGGNCCY 1 cut(s) 1801
EcoRI GAATTC 1 cut(s) 2284
EcoRII CCWGG 6 cut(s) 594, 1552, 1748, 1777, 1977, 2140
EcoRV GATATC 2 cut(s) 28, 3397
EcoT14I CCWWGG 2 cut(s) 988, 2597
EcoT22I ATGCAT 4 cut(s) 400, 1701, 2824, 2978
EcoT38I GRGCYC 3 cut(s) 1101, 2466, 2532
ErhI CCWWGG 2 cut(s) 988, 2597
Esp3I CGTCTC 1 cut(s) 3409
FalI AAGNNNNNCTT 4 cut(s) 1755, 1787, 2721, 2753
FaqI GGGAC 3 cut(s) 449, 634, 1742
FauI CCCGC 1 cut(s) 1426
FauNDI CATATG 1 cut(s) 1884
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 9 cut(s) 6, 9, 474, 754, 1680, 2269, 2314, 2629, 3005
FriOI GRGCYC 3 cut(s) 1101, 2466, 2532
Fsp4HI GCNGC 9 cut(s) 6, 9, 474, 754, 1680, 2269, 2314, 2629, 3005
FspBI CTAG 3 cut(s) 18, 1715, 2639
FspI TGCGCA 1 cut(s) 273
GlaI GCGC 2 cut(s) 273, 300
GluI GCNGC 9 cut(s) 6, 9, 474, 754, 1680, 2269, 2314, 2629, 3005
GsuI CTGGAG 4 cut(s) 854, 984, 1200, 1761
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 6 cut(s) 511, 594, 987, 1802, 2719, 3215
HapII CCGG 3 cut(s) 82, 652, 3246
HhaI GCGC 2 cut(s) 274, 301
Hin6I GCGC 2 cut(s) 272, 299
HinP1I GCGC 2 cut(s) 272, 299
HincII GTYRAC 1 cut(s) 2698
HindII GTYRAC 1 cut(s) 2698
HindIII AAGCTT 5 cut(s) 209, 747, 2591, 2927, 3457
HpaII CCGG 3 cut(s) 82, 652, 3246
HphI GGTGA 7 cut(s) 22, 85, 716, 980, 1265, 2693, 2738
Hpy166II GTNNAC 7 cut(s) 87, 794, 1598, 1746, 1768, 2698, 2774
Hpy8I GTNNAC 7 cut(s) 87, 794, 1598, 1746, 1768, 2698, 2774
Hpy99I CGWCG 1 cut(s) 1596
HpyCH4III ACNGT 7 cut(s) 327, 535, 728, 791, 1040, 2647, 3280
HpyCH4IV ACGT 6 cut(s) 418, 1080, 1671, 2170, 2633, 2827
HpySE526I ACGT 6 cut(s) 418, 1080, 1671, 2170, 2633, 2827
HspAI GCGC 2 cut(s) 272, 299
Kzo9I GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
LguI GCTCTTC 1 cut(s) 2724
LmnI GCTCC 9 cut(s) 1941, 2117, 2223, 2295, 2329, 2461, 2469, 2535, 2778
Lsp1109I GCAGC 6 cut(s) 485, 740, 1666, 2280, 2300, 2615
MaeI CTAG 3 cut(s) 18, 1715, 2639
MaeII ACGT 6 cut(s) 418, 1080, 1671, 2170, 2633, 2827
MaeIII GTNAC 6 cut(s) 1006, 1367, 2634, 2963, 3068, 3448
MalI GATC 9 cut(s) 357, 874, 1551, 1653, 1931, 1959, 1995, 2766, 3129
MboI GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
MfeI CAATTG 1 cut(s) 2431
MflI RGATCY 4 cut(s) 355, 1549, 1651, 1993
MhlI GDGCHC 7 cut(s) 447, 1038, 1101, 1770, 2466, 2532, 2776
MluI ACGCGT 1 cut(s) 1024
MlyI GAGTC 8 cut(s) 524, 656, 2014, 2383, 2449, 2515, 3309, 3437
MmeI TCCRAC 1 cut(s) 580
Mph1103I ATGCAT 4 cut(s) 400, 1701, 2824, 2978
MroXI GAANNNNTTC 3 cut(s) 419, 819, 1527
MslI CAYNNNNRTG 5 cut(s) 105, 1386, 1454, 1469, 1640
MspCI CTTAAG 2 cut(s) 2158, 2252
MspI CCGG 3 cut(s) 82, 652, 3246
MspR9I CCNGG 9 cut(s) 82, 596, 652, 653, 1554, 1750, 1779, 1979, 2142
MunI CAATTG 1 cut(s) 2431
Mva1269I GAATGC 2 cut(s) 1681, 2987
MvaI CCWGG 6 cut(s) 596, 1554, 1750, 1779, 1979, 2142
MvnI CGCG 2 cut(s) 1026, 3189
NciI CCSGG 3 cut(s) 82, 652, 653
NcoI CCATGG 1 cut(s) 2597
NdeI CATATG 1 cut(s) 1884
NdeII GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
NlaIV GGNNCC 4 cut(s) 1483, 1989, 3046, 3369
NmuCI GTSAC 3 cut(s) 1367, 2634, 3068
NruI TCGCGA 1 cut(s) 3189
NsbI TGCGCA 1 cut(s) 273
NsiI ATGCAT 4 cut(s) 400, 1701, 2824, 2978
NspV TTCGAA 2 cut(s) 744, 1689
PaeR7I CTCGAG 2 cut(s) 2015, 2210
PagI TCATGA 1 cut(s) 1641
PaqCI CACCTGC 1 cut(s) 187
PceI AGGCCT 1 cut(s) 3215
PciSI GCTCTTC 1 cut(s) 2724
PctI GAATGC 2 cut(s) 1681, 2987
PdmI GAANNNNTTC 3 cut(s) 419, 819, 1527
PfeI GAWTC 4 cut(s) 203, 311, 999, 2237
PfoI TCCNGGA 1 cut(s) 1552
PinAI ACCGGT 1 cut(s) 3245
PkrI GCNGC 9 cut(s) 7, 10, 475, 755, 1681, 2270, 2315, 2630, 3006
PleI GAGTC 8 cut(s) 523, 655, 2013, 2382, 2448, 2514, 3308, 3436
PpsI GAGTC 8 cut(s) 523, 655, 2013, 2382, 2448, 2514, 3308, 3436
Ppu21I YACGTR 1 cut(s) 1672
PshAI GACNNNNGTC 1 cut(s) 536
PshBI ATTAAT 3 cut(s) 1386, 2025, 3015
PsiI TTATAA 1 cut(s) 2747
Psp124BI GAGCTC 3 cut(s) 1101, 2466, 2532
Psp1406I AACGTT 1 cut(s) 418
Psp6I CCWGG 6 cut(s) 594, 1552, 1748, 1777, 1977, 2140
PspGI CCWGG 6 cut(s) 594, 1552, 1748, 1777, 1977, 2140
PspN4I GGNNCC 4 cut(s) 1483, 1989, 3046, 3369
PspPI GGNCC 4 cut(s) 510, 1801, 3023, 3248
PsuI RGATCY 4 cut(s) 355, 1549, 1651, 1993
RruI TCGCGA 1 cut(s) 3189
RseI CAYNNNNRTG 5 cut(s) 105, 1386, 1454, 1469, 1640
Rsr2I CGGWCCG 1 cut(s) 3248
RsrII CGGWCCG 1 cut(s) 3248
SacI GAGCTC 3 cut(s) 1101, 2466, 2532
SapI GCTCTTC 1 cut(s) 2724
SatI GCNGC 9 cut(s) 6, 9, 474, 754, 1680, 2269, 2314, 2629, 3005
Sau3AI GATC 9 cut(s) 355, 872, 1549, 1651, 1929, 1957, 1993, 2764, 3127
Sau96I GGNCC 4 cut(s) 510, 1801, 3023, 3248
SchI GAGTC 8 cut(s) 524, 656, 2014, 2383, 2449, 2515, 3309, 3437
ScrFI CCNGG 9 cut(s) 82, 596, 652, 653, 1554, 1750, 1779, 1979, 2142
SduI GDGCHC 7 cut(s) 447, 1038, 1101, 1770, 2466, 2532, 2776
SfcI CTRYAG 1 cut(s) 155
Sfr274I CTCGAG 2 cut(s) 2015, 2210
SfuI TTCGAA 2 cut(s) 744, 1689
SinI GGWCC 2 cut(s) 3023, 3248
SlaI CTCGAG 2 cut(s) 2015, 2210
SmaI CCCGGG 1 cut(s) 653
SmiMI CAYNNNNRTG 5 cut(s) 105, 1386, 1454, 1469, 1640
SseBI AGGCCT 1 cut(s) 3215
SsiI CCGC 3 cut(s) 1212, 1433, 3005
SspI AATATT 2 cut(s) 2260, 3019
SspMI CTAG 3 cut(s) 18, 1715, 2639
SstI GAGCTC 3 cut(s) 1101, 2466, 2532
StuI AGGCCT 1 cut(s) 3215
StyD4I CCNGG 9 cut(s) 80, 594, 650, 651, 1552, 1748, 1777, 1977, 2140
StyI CCWWGG 2 cut(s) 988, 2597
TaaI ACNGT 7 cut(s) 327, 535, 728, 791, 1040, 2647, 3280
TaiI ACGT 6 cut(s) 421, 1083, 1674, 2173, 2636, 2830
TatI WGTACW 7 cut(s) 367, 902, 1145, 1597, 2039, 2361, 3176
TauI GCSGC 1 cut(s) 3007
TfiI GAWTC 4 cut(s) 203, 311, 999, 2237
TscAI CASTG 5 cut(s) 205, 2358, 2842, 3283, 3445
TseFI GTSAC 3 cut(s) 1367, 2634, 3068
TseI GCWGC 8 cut(s) 5, 8, 473, 753, 1679, 2268, 2313, 2628
Tsp45I GTSAC 3 cut(s) 1367, 2634, 3068
TspGWI ACGGA 5 cut(s) 1459, 1474, 1664, 2210, 2791
TspMI CCCGGG 1 cut(s) 651
TspRI CASTG 5 cut(s) 205, 2358, 2842, 3283, 3445
Vha464I CTTAAG 2 cut(s) 2158, 2252
VneI GTGCAC 2 cut(s) 1766, 2772
VpaK11BI GGWCC 2 cut(s) 3023, 3248
VspI ATTAAT 3 cut(s) 1386, 2025, 3015
XagI CCTNNNNNAGG 1 cut(s) 1982
XapI RAATTY 9 cut(s) 629, 953, 1274, 1401, 2124, 2284, 2741, 2877, 3258
XcmI CCANNNNNNNNNTGG 1 cut(s) 3283
XhoI CTCGAG 2 cut(s) 2015, 2210
XmaI CCCGGG 1 cut(s) 651
XmiI GTMKAC 1 cut(s) 86
XmnI GAANNNNTTC 3 cut(s) 419, 819, 1527
XspI CTAG 3 cut(s) 18, 1715, 2639
Zsp2I ATGCAT 4 cut(s) 400, 1701, 2824, 2978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.