Rh5DG282200

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014255
Physical Location & Seq
Reverse (-)
34727460 .. 34734768
7309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG282200.1

Sequence Viewer

Length: 507 bp
ATGTCGAAGTGGTTGGGGAGGATTGCTGGGCTGTTCAGAAGTCGGAGTATGGTCGGAAAGGACAAGACCGGGAACCGTTACTTCACCAGAAAAGATGAAGTTGATGGCGTCTTGAAAGAGAAAAGATGGGTGGTATTCAAGGGTGAGGAGGACCCAACCTCCGTTCCAGTTGAATGGATATGTTGGCTCAACGGGCAGCGGAAAAAGGCTCCAACAACAGAGGAAATGATAGAATTGGAAGCAAAGCGTGAACGAGTCCGGCAAAATGTTGCTCTTCTGAAGAAAGAAGAAGAGGAAAGAAAAGCAAGAGAAGGCACTACACGCAAAGTCGTGAATACTGGTAAAGCTGGAGGTCCAGACTTGAGAAGTTTCATTCGCCAATTTCCTGGGTCCTCAGAAGGTGACGAACACAAGGAAGAAACCAATGAGATGGATGAAGTGAGGACCCCCAAAGAGAGAGACGCAGAAAAAGAAAAACTTTCGGAGTATGTCCTACTTGCACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

168

Amino Acids

19.47

Weight (kDa)

8.9

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 17 - 91 6.6e-13 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 108
AgsI TTSAA 3 cut(s) 115, 139, 173
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
Alw26I GTCTC 1 cut(s) 453
ApeKI GCWGC 1 cut(s) 196
AspS9I GGNCC 4 cut(s) 151, 353, 390, 444
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 3 cut(s) 76, 155, 413
AvaII GGWCC 4 cut(s) 151, 353, 390, 444
BbvI GCAGC 1 cut(s) 208
BccI CCATC 3 cut(s) 98, 120, 424
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 453
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
Bme1390I CCNGG 2 cut(s) 70, 387
Bme18I GGWCC 4 cut(s) 151, 353, 390, 444
BmgT120I GGNCC 4 cut(s) 151, 353, 390, 444
BmiI GGNNCC 5 cut(s) 74, 153, 210, 391, 446
BmrFI CCNGG 2 cut(s) 70, 387
BpmI CTGGAG 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bse1I ACTGG 2 cut(s) 167, 343
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 167, 343
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 26
BsiSI CCGG 2 cut(s) 69, 259
BsmAI GTCTC 1 cut(s) 453
BsmBI CGTCTC 1 cut(s) 453
BspACI CCGC 1 cut(s) 199
BspCNI CTCAG 1 cut(s) 407
BspLI GGNNCC 5 cut(s) 74, 153, 210, 391, 446
BspQI GCTCTTC 1 cut(s) 279
BsrI ACTGG 2 cut(s) 167, 343
BssECI CCNNGG 1 cut(s) 386
BssNI GRCGYC 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 279, 285
BstACI GRCGYC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 439
BstMAI GTCTC 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 193, 321
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 68, 385
BstV1I GCAGC 1 cut(s) 208
BstXI CCANNNNNNTGG 3 cut(s) 174, 386, 430
BtsCI GGATG 1 cut(s) 439
Cfr13I GGNCC 4 cut(s) 151, 353, 390, 444
CseI GACGC 2 cut(s) 97, 470
CviJI RGCY 4 cut(s) 31, 187, 209, 347
CviKI_1 RGCY 4 cut(s) 31, 187, 209, 347
DdeI CTNAG 1 cut(s) 394
Eam1104I CTCTTC 2 cut(s) 279, 285
EarI CTCTTC 2 cut(s) 279, 285
Eco47I GGWCC 4 cut(s) 151, 353, 390, 444
Eco57I CTGAAG 1 cut(s) 299
EcoO109I RGGNCCY 3 cut(s) 151, 390, 444
EcoRII CCWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 453
FaiI YATR 3 cut(s) 50, 181, 489
FalI AAGNNNNNCTT 2 cut(s) 462, 494
Fnu4HI GCNGC 1 cut(s) 197
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 197
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 369
HapII CCGG 2 cut(s) 69, 259
HgaI GACGC 2 cut(s) 97, 470
Hin1I GRCGYC 1 cut(s) 108
HinfI GANTC 1 cut(s) 255
HpaII CCGG 2 cut(s) 69, 259
HphI GGTGA 3 cut(s) 76, 155, 413
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 6 cut(s) 38, 45, 56, 279, 397, 484
Hpy188III TCNNGA 3 cut(s) 112, 331, 356
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 2 cut(s) 305, 392
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 1 cut(s) 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 321
HpyF3I CTNAG 1 cut(s) 394
Hsp92I GRCGYC 1 cut(s) 108
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 208
MaeIII GTNAC 2 cut(s) 77, 401
MboII GAAGA 5 cut(s) 266, 292, 299, 302, 428
MluCI AATT 2 cut(s) 233, 380
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 3 cut(s) 23, 34, 236
MnlI CCTC 9 cut(s) 12, 139, 142, 169, 214, 286, 344, 403, 435
MseI TTAA 1 cut(s) 505
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 2 cut(s) 69, 259
MspR9I CCNGG 2 cut(s) 70, 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 193, 321
NciI CCSGG 1 cut(s) 70
NlaIV GGNNCC 5 cut(s) 74, 153, 210, 391, 446
NmuCI GTSAC 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 279
PkrI GCNGC 1 cut(s) 198
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 3 cut(s) 151, 390, 444
Psp5II RGGWCCY 3 cut(s) 151, 390, 444
Psp6I CCWGG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 5 cut(s) 74, 153, 210, 391, 446
PspPI GGNCC 4 cut(s) 151, 353, 390, 444
PspPPI RGGWCCY 3 cut(s) 151, 390, 444
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 1 cut(s) 505
SatI GCNGC 1 cut(s) 197
Sau96I GGNCC 4 cut(s) 151, 353, 390, 444
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 2 cut(s) 70, 387
SetI ASST 5 cut(s) 161, 349, 355, 403, 505
SinI GGWCC 4 cut(s) 151, 353, 390, 444
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 2 cut(s) 233, 380
SsiI CCGC 1 cut(s) 199
StyD4I CCNGG 2 cut(s) 68, 385
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 5
TasI AATT 2 cut(s) 233, 380
Tru1I TTAA 1 cut(s) 505
Tru9I TTAA 1 cut(s) 505
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 111, 361, 450
TspGWI ACGGA 1 cut(s) 151
VpaK11BI GGWCC 4 cut(s) 151, 353, 390, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.