FvH4_3g22910

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013358
Physical Location & Seq
Forward (+)
16042754 .. 16044518
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22910.t1

Sequence Viewer

Length: 558 bp
ATGAGAGGAAACTCAGATGACAATGCCCCAAATATCCCTCTTCTGAAGCTCATAGATTGCTCCCTTAGGGTTTCTGTGATTCCTCTTAGTATTGCAACTATATGGTTGACTGTGACCAACCACCAGGACAACACTAGCTATGGGAAGCTGCACTTCAGCAATTTCATGGGCCTCAAGTACATGGTTTGGATCAGCGCCATTTCTGCTGTATATGCTTGTGTTGCTGCTGTGTCCTCATGGATCAAATGCTTAGTCTCTAAAGCTTGGCTTTTCTTTGTCTCTGACCAGATAGTGACCTACTTAATGGTCACATCTGTGGCTGCAGTGATGGAGATGGTGTTTCTGGCTTATAAAGGTGACAAGGCAGTCTCATGGAGTGAAGCTTGTGCTTCATATGGGAAATTCTGCAGCAGAATGAAATTGGCCTTGGTTCTCCATTCTCTTGCCCTTTTTAGCTTCCTTGTTTTAGCTGTGATTTCTGCTTATAGGGTCTTCAGCATGTGTGAGGCACCTTCTGTTTCTCACAAAGAGGTGGAGTTAGAAGAGAACACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

186

Amino Acids

20.5

Weight (kDa)

7.6

Isoelectric Point (pI)

20.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 15 - 151 1.9e-29 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013358)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54980
fragaria_vesca FvH4_3g22910
malus_domestica MD03G1199400.v1.1 MD11G1216200.v1.1 MD11G1216300.v1.1
prunus_persica Prupe.4G215200_v2.0.a1
pyrus_communis pycom03g15100
rosa_chinensis RchiOBHm_Chr5g0040241
rosa_laevigata RLG00000033966
rosa_multiflora Rmu_sc0002908.1_g000012 Rmu_sc0010162.1_g000006
rosa_roxburghii Rroxscaffold_1G00041070
rosa_rugosa Rorug05G0184200
rosa_samantha Rh5AG271100 Rh5BG274400 Rh5CG307300 Rh5DG282600
rosa_wichuraiana Rw5G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 508
AclWI GGATC 2 cut(s) 197, 248
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 3 cut(s) 65, 139, 478
AfaI GTAC 1 cut(s) 179
AjnI CCWGG 1 cut(s) 123
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 7 cut(s) 49, 138, 148, 263, 383, 456, 470
AluI AGCT 7 cut(s) 49, 138, 148, 263, 383, 456, 470
Alw26I GTCTC 3 cut(s) 259, 283, 373
AlwI GGATC 2 cut(s) 197, 248
AoxI GGCC 2 cut(s) 169, 423
ApeKI GCWGC 4 cut(s) 148, 224, 320, 408
ApoI RAATTY 1 cut(s) 401
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 65
BanI GGYRCC 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 484
BbvI GCAGC 4 cut(s) 135, 211, 307, 420
BccI CCATC 2 cut(s) 322, 328
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 3 cut(s) 259, 283, 373
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 2 cut(s) 321, 406
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 4 cut(s) 149, 225, 321, 409
BlsI GCNGC 4 cut(s) 150, 226, 322, 410
Bme1390I CCNGG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 426
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bse21I CCTNAGG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 27
BseXI GCAGC 4 cut(s) 135, 211, 307, 420
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 2 cut(s) 171, 425
BshNI GGYRCC 1 cut(s) 508
BsmAI GTCTC 3 cut(s) 259, 283, 373
BsnI GGCC 2 cut(s) 171, 425
Bsp143I GATC 2 cut(s) 189, 240
BspANI GGCC 2 cut(s) 171, 425
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 510
BspMAI CTGCAG 2 cut(s) 325, 410
BspPI GGATC 2 cut(s) 197, 248
BspT107I GGYRCC 1 cut(s) 508
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 2 cut(s) 189, 240
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 45, 537
BstDEI CTNAG 5 cut(s) 13, 65, 86, 250, 555
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 2 cut(s) 192, 243
BstMAI GTCTC 3 cut(s) 259, 283, 373
BstMBI GATC 2 cut(s) 189, 240
BstMWI GCNNNNNNNGC 3 cut(s) 203, 212, 221
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 2 cut(s) 321, 406
BstV1I GCAGC 4 cut(s) 135, 211, 307, 420
BstV2I GAAGAC 1 cut(s) 484
Bsu36I CCTNAGG 1 cut(s) 65
BsuRI GGCC 2 cut(s) 171, 425
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 169
Csp6I GTAC 1 cut(s) 178
CviAII CATG 5 cut(s) 166, 181, 237, 372, 499
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 5 cut(s) 13, 65, 86, 250, 555
DpnI GATC 2 cut(s) 191, 242
DpnII GATC 2 cut(s) 189, 240
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
Eam1104I CTCTTC 2 cut(s) 45, 537
EarI CTCTTC 2 cut(s) 45, 537
Eco130I CCWWGG 1 cut(s) 426
Eco57I CTGAAG 3 cut(s) 65, 139, 478
Eco81I CCTNAGG 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 5 cut(s) 169, 184, 240, 375, 502
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 252, 284
FatI CATG 5 cut(s) 165, 180, 236, 371, 498
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 4 cut(s) 149, 225, 321, 409
Fsp4HI GCNGC 4 cut(s) 149, 225, 321, 409
FspBI CTAG 1 cut(s) 135
GlaI GCGC 1 cut(s) 196
GluI GCNGC 4 cut(s) 149, 225, 321, 409
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 2 cut(s) 171, 425
HhaI GCGC 1 cut(s) 197
Hin1II CATG 5 cut(s) 169, 184, 240, 375, 502
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 2 cut(s) 261, 381
HinfI GANTC 1 cut(s) 79
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 3 cut(s) 16, 45, 283
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 522
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 95, 151, 323, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 212, 221
HpyF3I CTNAG 5 cut(s) 13, 65, 86, 250, 555
Hsp92II CATG 5 cut(s) 169, 184, 240, 375, 502
HspAI GCGC 1 cut(s) 195
Kzo9I GATC 2 cut(s) 189, 240
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 4 cut(s) 110, 137, 299, 329
Lsp1109I GCAGC 4 cut(s) 135, 211, 307, 420
MaeI CTAG 1 cut(s) 135
MaeIII GTNAC 4 cut(s) 112, 292, 307, 356
MalI GATC 2 cut(s) 191, 242
MboI GATC 2 cut(s) 189, 240
MboII GAAGA 3 cut(s) 32, 484, 554
MluCI AATT 3 cut(s) 160, 401, 419
MnlI CCTC 6 cut(s) 48, 93, 182, 244, 499, 523
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 314
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 203, 212, 221
NdeI CATATG 1 cut(s) 394
NdeII GATC 2 cut(s) 189, 240
NlaIII CATG 5 cut(s) 169, 184, 240, 375, 502
NlaIV GGNNCC 1 cut(s) 510
NmuCI GTSAC 4 cut(s) 112, 292, 307, 356
NspI RCATGY 1 cut(s) 502
OliI CACNNNNGTG 1 cut(s) 314
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 4 cut(s) 150, 226, 322, 410
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 1 cut(s) 169
PstI CTGCAG 2 cut(s) 325, 410
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 4 cut(s) 149, 225, 321, 409
Sau3AI GATC 2 cut(s) 189, 240
Sau96I GGNCC 1 cut(s) 169
ScrFI CCNGG 1 cut(s) 125
SfcI CTRYAG 2 cut(s) 321, 406
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 3 cut(s) 160, 401, 419
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 112
TasI AATT 3 cut(s) 160, 401, 419
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 4 cut(s) 112, 292, 307, 356
TseI GCWGC 4 cut(s) 148, 224, 320, 408
Tsp45I GTSAC 4 cut(s) 112, 292, 307, 356
TspDTI ATGAA 3 cut(s) 154, 381, 431
TspRI CASTG 1 cut(s) 330
XapI RAATTY 1 cut(s) 401
XceI RCATGY 1 cut(s) 502
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.